9000894

1-(2-fluorophenyl)-3-(4-methyl-3-nitrophenyl)urea

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2843
Screen concentration 45.5 μM
Source Chembridge (Drug-like library)
PubChem CID 2242070
SMILES CC1=C(C=C(C=C1)NC(=O)NC2=CC=CC=C2F)[N+](=O)[O-]
Standardized SMILES Cc1ccc(NC(=O)Nc2ccccc2F)cc1[N+](=O)[O-]
Molecular weight 289.2618
ALogP 3.11
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.42
% growth inhibition (Hom. pool) 4.36


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2242070
Download HIP data (tab-delimited text)  (excel)
Gene:ALA1(YOR335C)|FD-Score:4.68|P-value:1.46E-6|Clearance:0.84||SGD DESC:Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog Gene:FAP7(YDL166C)|FD-Score:-3.38|P-value:3.64E-4|Clearance:0||SGD DESC:Essential NTPase required for small ribosome subunit synthesis, mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D Gene:HRT1(YOL133W)|FD-Score:3.31|P-value:4.59E-4|Clearance:0.12||SGD DESC:RING finger containing subunit of Skp1-Cullin-F-box ubiquitin protein ligases (SCF); required for Gic2p, Far1p, Sic1p and Cln2p degradation; may tether Cdc34p (a ubiquitin conjugating enzyme or E2) and Cdc53p (a cullin) subunits of SCF Gene:NOP53(YPL146C)|FD-Score:3.83|P-value:6.28E-5|Clearance:0.32||SGD DESC:Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired Gene:PSA1(YDL055C)|FD-Score:-3.11|P-value:9.32E-4|Clearance:0||SGD DESC:GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from GTP and mannose-1-phosphate in cell wall biosynthesis; required for normal cell wall structure Gene:RLP24(YLR009W)|FD-Score:3.51|P-value:2.23E-4|Clearance:0.2||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPT3(YDR394W)|FD-Score:-3.62|P-value:1.48E-4|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:RSC8(YFR037C)|FD-Score:-3.39|P-value:3.52E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:TEM1(YML064C)|FD-Score:-3.43|P-value:3.04E-4|Clearance:0||SGD DESC:GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis Gene:UTP5(YDR398W)|FD-Score:3.15|P-value:8.13E-4|Clearance:0.2||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:YRB1(YDR002W)|FD-Score:3.19|P-value:7.06E-4|Clearance:0.04||SGD DESC:Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1 Gene:ALA1(YOR335C)|FD-Score:4.68|P-value:1.46E-6|Clearance:0.84||SGD DESC:Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog Gene:FAP7(YDL166C)|FD-Score:-3.38|P-value:3.64E-4|Clearance:0||SGD DESC:Essential NTPase required for small ribosome subunit synthesis, mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D Gene:HRT1(YOL133W)|FD-Score:3.31|P-value:4.59E-4|Clearance:0.12||SGD DESC:RING finger containing subunit of Skp1-Cullin-F-box ubiquitin protein ligases (SCF); required for Gic2p, Far1p, Sic1p and Cln2p degradation; may tether Cdc34p (a ubiquitin conjugating enzyme or E2) and Cdc53p (a cullin) subunits of SCF Gene:NOP53(YPL146C)|FD-Score:3.83|P-value:6.28E-5|Clearance:0.32||SGD DESC:Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired Gene:PSA1(YDL055C)|FD-Score:-3.11|P-value:9.32E-4|Clearance:0||SGD DESC:GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from GTP and mannose-1-phosphate in cell wall biosynthesis; required for normal cell wall structure Gene:RLP24(YLR009W)|FD-Score:3.51|P-value:2.23E-4|Clearance:0.2||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPT3(YDR394W)|FD-Score:-3.62|P-value:1.48E-4|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:RSC8(YFR037C)|FD-Score:-3.39|P-value:3.52E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:TEM1(YML064C)|FD-Score:-3.43|P-value:3.04E-4|Clearance:0||SGD DESC:GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis Gene:UTP5(YDR398W)|FD-Score:3.15|P-value:8.13E-4|Clearance:0.2||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:YRB1(YDR002W)|FD-Score:3.19|P-value:7.06E-4|Clearance:0.04||SGD DESC:Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2242070
Download HOP data (tab-delimited text)  (excel)
Gene:ATG27(YJL178C)|FD-Score:3.79|P-value:7.60E-5||SGD DESC:Type I membrane protein involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; may be involved in membrane delivery to the phagophore assembly site Gene:BUD27(YFL023W)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:COX6(YHR051W)|FD-Score:3.1|P-value:9.71E-4||SGD DESC:Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels Gene:DUG1(YFR044C)|FD-Score:-3.6|P-value:1.59E-4||SGD DESC:Cys-Gly metallo-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:FAA3(YIL009W)|FD-Score:4.72|P-value:1.19E-6||SGD DESC:Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:GUF1(YLR289W)|FD-Score:4.06|P-value:2.48E-5||SGD DESC:Mitochondrial matrix GTPase that associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor Gene:HOG1(YLR113W)|FD-Score:-3.47|P-value:2.59E-4||SGD DESC:Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNA Pol II in response to osmotic shock; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p; protein abundance increases in response to DNA replication stress Gene:IRC22(YEL001C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; YEL001C is non-essential; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRC5(YFR038W)|FD-Score:-3.43|P-value:2.96E-4||SGD DESC:Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci Gene:ITT1(YML068W)|FD-Score:5.78|P-value:3.83E-9||SGD DESC:Protein that modulates the efficiency of translation termination, interacts with translation release factors eRF1 (Sup45p) and eRF3 (Sup35p) in vitro, contains a zinc finger domain characteristic of the TRIAD class of proteins Gene:LAP3(YNL239W)|FD-Score:3.2|P-value:6.99E-4||SGD DESC:Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH Gene:MAL12(YGR292W)|FD-Score:3.81|P-value:6.96E-5||SGD DESC:Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL1 complex locus; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose Gene:MCH1(YDL054C)|FD-Score:-3.84|P-value:6.07E-5||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MDG1(YNL173C)|FD-Score:-3.35|P-value:4.00E-4||SGD DESC:Plasma membrane protein involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations Gene:MET22(YOL064C)|FD-Score:4.57|P-value:2.43E-6||SGD DESC:Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway Gene:MIC17(YMR002W)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Mitochondrial intermembrane space protein; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MPC2(YHR162W)|FD-Score:-3.12|P-value:9.01E-4||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium Gene:MRH4(YGL064C)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function Gene:POC4(YPL144W)|FD-Score:4.33|P-value:7.38E-6||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:RAD24(YER173W)|FD-Score:-4.29|P-value:8.79E-6||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein Gene:SFA1(YDL168W)|FD-Score:-3.61|P-value:1.55E-4||SGD DESC:Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress Gene:SHG1(YBR258C)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres Gene:SPE2(YOL052C)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:S-adenosylmethionine decarboxylase, required for the biosynthesis of spermidine and spermine; cells lacking Spe2p require spermine or spermidine for growth in the presence of oxygen but not when grown anaerobically Gene:SPT4(YGR063C)|FD-Score:4.38|P-value:5.90E-6||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SSD1(YDR293C)|FD-Score:3.16|P-value:7.91E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SWF1(YDR126W)|FD-Score:4.52|P-value:3.12E-6||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:TGL3(YMR313C)|FD-Score:3.19|P-value:7.03E-4||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidylethanolamine acyltransferase activity; responsible for all the triacylglycerol lipase activity of the lipid particle; required with Tgl4p for timely bud formation Gene:UBP15(YMR304W)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Ubiquitin-specific protease involved in protein deubiquitination; catalytic activity regulated by an N-terminal TRAF-like domain and and C-terminal sequences; physically interacts with anaphase-promoting complex/cyclosome (APC/C) activator, Cdh1p; forms a complex with AAA peroxins Pex1p and Pex6p Gene:VAB2(YEL005C)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:Protein with a potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of BLOC complex involved in endosomal cargo sorting; Vab2p-GFP-fusion localizes to cytoplasm in punctate patter Gene:VPS33(YLR396C)|FD-Score:-6.63|P-value:1.71E-11||SGD DESC:ATP-binding protein that is a subunit of the HOPS complex and of the CORVET tethering complex; essential for protein sorting, vesicle docking and fusion at the vacuole Gene:YBL062W(YBL062W_d)|FD-Score:4.66|P-value:1.56E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR085C-A(YBR085C-A_p)|FD-Score:3.86|P-value:5.74E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus; protein abundance increases in response to DNA replication stress Gene:YDR250C(YDR250C_d)|FD-Score:4.32|P-value:7.66E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YEL025C(YEL025C_p)|FD-Score:4.73|P-value:1.11E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YIL058W(YIL058W_d)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL102C(YIL102C_p)|FD-Score:3.99|P-value:3.30E-5||SGD DESC:Putative protein of unknown function Gene:YTP1(YNL237W)|FD-Score:-3.79|P-value:7.40E-5||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins Gene:ATG27(YJL178C)|FD-Score:3.79|P-value:7.60E-5||SGD DESC:Type I membrane protein involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; may be involved in membrane delivery to the phagophore assembly site Gene:BUD27(YFL023W)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:COX6(YHR051W)|FD-Score:3.1|P-value:9.71E-4||SGD DESC:Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels Gene:DUG1(YFR044C)|FD-Score:-3.6|P-value:1.59E-4||SGD DESC:Cys-Gly metallo-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:FAA3(YIL009W)|FD-Score:4.72|P-value:1.19E-6||SGD DESC:Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:GUF1(YLR289W)|FD-Score:4.06|P-value:2.48E-5||SGD DESC:Mitochondrial matrix GTPase that associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor Gene:HOG1(YLR113W)|FD-Score:-3.47|P-value:2.59E-4||SGD DESC:Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNA Pol II in response to osmotic shock; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p; protein abundance increases in response to DNA replication stress Gene:IRC22(YEL001C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; YEL001C is non-essential; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRC5(YFR038W)|FD-Score:-3.43|P-value:2.96E-4||SGD DESC:Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci Gene:ITT1(YML068W)|FD-Score:5.78|P-value:3.83E-9||SGD DESC:Protein that modulates the efficiency of translation termination, interacts with translation release factors eRF1 (Sup45p) and eRF3 (Sup35p) in vitro, contains a zinc finger domain characteristic of the TRIAD class of proteins Gene:LAP3(YNL239W)|FD-Score:3.2|P-value:6.99E-4||SGD DESC:Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH Gene:MAL12(YGR292W)|FD-Score:3.81|P-value:6.96E-5||SGD DESC:Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL1 complex locus; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose Gene:MCH1(YDL054C)|FD-Score:-3.84|P-value:6.07E-5||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MDG1(YNL173C)|FD-Score:-3.35|P-value:4.00E-4||SGD DESC:Plasma membrane protein involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations Gene:MET22(YOL064C)|FD-Score:4.57|P-value:2.43E-6||SGD DESC:Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway Gene:MIC17(YMR002W)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Mitochondrial intermembrane space protein; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MPC2(YHR162W)|FD-Score:-3.12|P-value:9.01E-4||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium Gene:MRH4(YGL064C)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function Gene:POC4(YPL144W)|FD-Score:4.33|P-value:7.38E-6||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:RAD24(YER173W)|FD-Score:-4.29|P-value:8.79E-6||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein Gene:SFA1(YDL168W)|FD-Score:-3.61|P-value:1.55E-4||SGD DESC:Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress Gene:SHG1(YBR258C)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres Gene:SPE2(YOL052C)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:S-adenosylmethionine decarboxylase, required for the biosynthesis of spermidine and spermine; cells lacking Spe2p require spermine or spermidine for growth in the presence of oxygen but not when grown anaerobically Gene:SPT4(YGR063C)|FD-Score:4.38|P-value:5.90E-6||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SSD1(YDR293C)|FD-Score:3.16|P-value:7.91E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SWF1(YDR126W)|FD-Score:4.52|P-value:3.12E-6||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:TGL3(YMR313C)|FD-Score:3.19|P-value:7.03E-4||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidylethanolamine acyltransferase activity; responsible for all the triacylglycerol lipase activity of the lipid particle; required with Tgl4p for timely bud formation Gene:UBP15(YMR304W)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Ubiquitin-specific protease involved in protein deubiquitination; catalytic activity regulated by an N-terminal TRAF-like domain and and C-terminal sequences; physically interacts with anaphase-promoting complex/cyclosome (APC/C) activator, Cdh1p; forms a complex with AAA peroxins Pex1p and Pex6p Gene:VAB2(YEL005C)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:Protein with a potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of BLOC complex involved in endosomal cargo sorting; Vab2p-GFP-fusion localizes to cytoplasm in punctate patter Gene:VPS33(YLR396C)|FD-Score:-6.63|P-value:1.71E-11||SGD DESC:ATP-binding protein that is a subunit of the HOPS complex and of the CORVET tethering complex; essential for protein sorting, vesicle docking and fusion at the vacuole Gene:YBL062W(YBL062W_d)|FD-Score:4.66|P-value:1.56E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR085C-A(YBR085C-A_p)|FD-Score:3.86|P-value:5.74E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus; protein abundance increases in response to DNA replication stress Gene:YDR250C(YDR250C_d)|FD-Score:4.32|P-value:7.66E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YEL025C(YEL025C_p)|FD-Score:4.73|P-value:1.11E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YIL058W(YIL058W_d)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL102C(YIL102C_p)|FD-Score:3.99|P-value:3.30E-5||SGD DESC:Putative protein of unknown function Gene:YTP1(YNL237W)|FD-Score:-3.79|P-value:7.40E-5||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR335C4.681.46E-60.84ALA1Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog
YPL146C3.836.28E-50.32NOP53Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired
YLR009W3.512.23E-40.20RLP24Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
YOL133W3.314.59E-40.12HRT1RING finger containing subunit of Skp1-Cullin-F-box ubiquitin protein ligases (SCF); required for Gic2p, Far1p, Sic1p and Cln2p degradation; may tether Cdc34p (a ubiquitin conjugating enzyme or E2) and Cdc53p (a cullin) subunits of SCF
YDR002W3.197.06E-40.04YRB1Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1
YDR398W3.158.13E-40.20UTP5Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit
YOR060C2.950.001600.23SLD7Protein with a role in chromosomal DNA replication; interacts with Sld3p and reduces its affinity for Cdc45p; deletion mutant has aberrant mitochondria
YDR041W2.720.003290.05RSM10Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins
YPL251W_d2.670.003800.20YPL251W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C
YEL035C_p2.470.006770.08UTR5_pProtein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions
YOL134C_d2.390.008400.18YOL134C_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps HRT1, a verified gene that encodes an SCF ubiquitin ligase subunit
YPR107C2.210.013600.04YTH1Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
YLR212C2.170.015100.01TUB4Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress
YHR042W2.160.015600.06NCP1NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p
YMR281W2.100.018000.02GPI12ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YML068W5.783.83E-9ITT1Protein that modulates the efficiency of translation termination, interacts with translation release factors eRF1 (Sup45p) and eRF3 (Sup35p) in vitro, contains a zinc finger domain characteristic of the TRIAD class of proteins
YEL025C_p4.731.11E-6YEL025C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
YIL009W4.721.19E-6FAA3Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
YBL062W_d4.661.56E-6YBL062W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YOL064C4.572.43E-6MET22Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway
YDR126W4.523.12E-6SWF1Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion
YGR063C4.385.90E-6SPT4Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions
YPL144W4.337.38E-6POC4Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions
YDR250C_d4.327.66E-6YDR250C_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data
YLR289W4.062.48E-5GUF1Mitochondrial matrix GTPase that associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor
YIL102C_p3.993.30E-5YIL102C_pPutative protein of unknown function
YBR085C-A_p3.865.74E-5YBR085C-A_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus; protein abundance increases in response to DNA replication stress
YGR292W3.816.96E-5MAL12Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL1 complex locus; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose
YJL178C3.797.60E-5ATG27Type I membrane protein involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; may be involved in membrane delivery to the phagophore assembly site
YEL001C3.641.35E-4IRC22Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; YEL001C is non-essential; null mutant displays increased levels of spontaneous Rad52p foci

GO enrichment analysis for SGTC_2843
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2321.06E-72SGTC_28399003951 26.0 μMChembridge (Drug-like library)29906340.215385
0.1783.79E-43SGTC_28479005661 71.4 μMChembridge (Drug-like library)29914490.21875
0.1462.42E-29SGTC_28409008771 71.4 μMChembridge (Drug-like library)29929780.16
0.0822.98E-10SGTC_20345197015 53.1 μMChembridge (Fragment library)7806760.1875SWF1 & branched chain AA biosynthesis
0.0814.22E-10SGTC_1696st025591 34.8 μMTimTec (Natural product derivative library)27871470.25SWF1 & branched chain AA biosynthesis
0.0809.09E-10SGTC_23387999974 200.0 μMChembridge (Fragment library)3159250.0714286
0.0791.40E-9SGTC_340469-0683 81.8 μMChemDiv (Drug-like library)36909230.407407SWF1 & branched chain AA biosynthesis
0.0723.32E-8SGTC_1570verbenone 133.0 μMTimTec (Pure natural product library)290250.0862069
0.0714.82E-8SGTC_575k072-0246 11.9 μMChemDiv (Drug-like library)67967790.144737
0.0691.15E-7SGTC_8420271-0008 11.1 μMChemDiv (Drug-like library)12217090.0983607heme biosynthesis & mitochondrial translocase
0.0691.29E-7SGTC_28459003024 43.0 μMChembridge (Drug-like library)29901730.19697amide catabolism
0.0673.00E-7SGTC_1070358-0022 2.7 μMChemDiv (Drug-like library)5211060.0877193SWF1 & branched chain AA biosynthesis
0.0631.44E-6SGTC_7961234-0020 70.7 μMChemDiv (Drug-like library)464950340.111111SWF1 & branched chain AA biosynthesis
0.0604.59E-6SGTC_20435101342 149.0 μMChembridge (Fragment library)6784510.107692SWF1 & branched chain AA biosynthesis
0.0595.61E-6SGTC_1714st033236 13.6 μMTimTec (Natural product derivative library)29165660.0933333

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_340469-068381.75 μM0.4074073690923ChemDiv (Drug-like library)337.307942.59217SWF1 & branched chain AA biosynthesis
SGTC_2856902292519.48 μM0.4035094944862Chembridge (Drug-like library)287.3287234.11213
SGTC_1703st02698348.3 μM0.3970591104023TimTec (Natural product derivative library)414.410125.02915
SGTC_8991083-0020478 μM0.360656767123ChemDiv (Drug-like library)306.701143.15714
SGTC_1764st04450293.5 μM0.3541678407TimTec (Natural product derivative library)214.223.27413
SGTC_10024049-0206172 μM0.3508774662749ChemDiv (Drug-like library)310.3008432.90316
SGTC_2878904397325.97 μM0.356465167Chembridge (Drug-like library)300.309242.62234
SGTC_14284049-020461.7 μM0.344828X1428ChemDiv (Drug-like library)324.327423.38941
SGTC_2798781363745.45 μM0.344828956368Chembridge (Drug-like library)277.2660263.34714
SGTC_6260214-000933 μM0.3392863437874ChemDiv (Drug-like library)332.225222.29717copper-dependent oxidative stress