1486-1325

1-(4-chlorophenyl)-3-[(4-methylphenyl)carbamoyl]-1-(4-methylphenyl)sulfonylurea

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_285
Screen concentration 46.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 22310230
SMILES CC1=CC=C(C=C1)NC(=O)NC(=O)N(C2=CC=C(C=C2)Cl)S(=O)(=O)C3=CC=C(C=C3)C
Standardized SMILES Cc1ccc(NC(=O)NC(=O)N(c2ccc(Cl)cc2)S(=O)(=O)c3ccc(C)cc3)cc1
Molecular weight 457.9299
ALogP 5.3
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 38.93
% growth inhibition (Hom. pool) 13.7


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 22310230
Download HIP data (tab-delimited text)  (excel)
Gene:ARC35(YNR035C)|FD-Score:8.14|P-value:1.93E-16|Clearance:2.72||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin Gene:GLC7(YER133W)|FD-Score:3.71|P-value:1.02E-4|Clearance:0.01||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:NET1(YJL076W)|FD-Score:3.74|P-value:9.07E-5|Clearance:0.03||SGD DESC:Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication Gene:NUP85(YJR042W)|FD-Score:3.21|P-value:6.71E-4|Clearance:0.02||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 Gene:RLP24(YLR009W)|FD-Score:3.71|P-value:1.04E-4|Clearance:0.32||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPB8(YOR224C)|FD-Score:3.29|P-value:5.05E-4|Clearance:0.08||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPL17A(YKL180W)|FD-Score:4.33|P-value:7.46E-6|Clearance:0.52||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPL18A(YOL120C)|FD-Score:3.13|P-value:8.75E-4|Clearance:0.05||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPP1(YHR062C)|FD-Score:5.42|P-value:3.01E-8|Clearance:0.46||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP4(YHR069C)|FD-Score:4.96|P-value:3.48E-7|Clearance:0.63||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:RRP46(YGR095C)|FD-Score:3.14|P-value:8.39E-4|Clearance:0.01||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:SCC4(YER147C)|FD-Score:3.33|P-value:4.39E-4|Clearance:0.04||SGD DESC:Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during double-strand break repair via phosphorylated histone H2AX Gene:SPC105(YGL093W)|FD-Score:-3.21|P-value:6.56E-4|Clearance:0||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SPN1(YPR133C)|FD-Score:3.18|P-value:7.31E-4|Clearance:0.04||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:STU1(YBL034C)|FD-Score:-3.32|P-value:4.53E-4|Clearance:0||SGD DESC:Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles Gene:UTP22(YGR090W)|FD-Score:-3.21|P-value:6.54E-4|Clearance:0||SGD DESC:Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals Gene:YGL102C(YGL102C_d)|FD-Score:3.81|P-value:6.90E-5|Clearance:0.07||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential RPL28 gene encoding a large subunit ribosomal protein Gene:YOR102W(YOR102W_d)|FD-Score:3.38|P-value:3.58E-4|Clearance:0.06||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex Gene:ARC35(YNR035C)|FD-Score:8.14|P-value:1.93E-16|Clearance:2.72||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin Gene:GLC7(YER133W)|FD-Score:3.71|P-value:1.02E-4|Clearance:0.01||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:NET1(YJL076W)|FD-Score:3.74|P-value:9.07E-5|Clearance:0.03||SGD DESC:Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication Gene:NUP85(YJR042W)|FD-Score:3.21|P-value:6.71E-4|Clearance:0.02||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 Gene:RLP24(YLR009W)|FD-Score:3.71|P-value:1.04E-4|Clearance:0.32||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPB8(YOR224C)|FD-Score:3.29|P-value:5.05E-4|Clearance:0.08||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPL17A(YKL180W)|FD-Score:4.33|P-value:7.46E-6|Clearance:0.52||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPL18A(YOL120C)|FD-Score:3.13|P-value:8.75E-4|Clearance:0.05||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPP1(YHR062C)|FD-Score:5.42|P-value:3.01E-8|Clearance:0.46||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP4(YHR069C)|FD-Score:4.96|P-value:3.48E-7|Clearance:0.63||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:RRP46(YGR095C)|FD-Score:3.14|P-value:8.39E-4|Clearance:0.01||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:SCC4(YER147C)|FD-Score:3.33|P-value:4.39E-4|Clearance:0.04||SGD DESC:Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during double-strand break repair via phosphorylated histone H2AX Gene:SPC105(YGL093W)|FD-Score:-3.21|P-value:6.56E-4|Clearance:0||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SPN1(YPR133C)|FD-Score:3.18|P-value:7.31E-4|Clearance:0.04||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:STU1(YBL034C)|FD-Score:-3.32|P-value:4.53E-4|Clearance:0||SGD DESC:Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles Gene:UTP22(YGR090W)|FD-Score:-3.21|P-value:6.54E-4|Clearance:0||SGD DESC:Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals Gene:YGL102C(YGL102C_d)|FD-Score:3.81|P-value:6.90E-5|Clearance:0.07||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential RPL28 gene encoding a large subunit ribosomal protein Gene:YOR102W(YOR102W_d)|FD-Score:3.38|P-value:3.58E-4|Clearance:0.06||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 22310230
Download HOP data (tab-delimited text)  (excel)
Gene:ARG3(YJL088W)|FD-Score:5.47|P-value:2.27E-8||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ARO1(YDR127W)|FD-Score:4.35|P-value:6.78E-6||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:8.55|P-value:6.42E-18||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:BTS1(YPL069C)|FD-Score:-3.35|P-value:4.05E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CWP2(YKL096W-A)|FD-Score:3.23|P-value:6.09E-4||SGD DESC:Covalently linked cell wall mannoprotein, major constituent of the cell wall; plays a role in stabilizing the cell wall; involved in low pH resistance; precursor is GPI-anchored Gene:DYN2(YDR424C)|FD-Score:3.82|P-value:6.75E-5||SGD DESC:Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex Gene:ERG2(YMR202W)|FD-Score:7.44|P-value:5.06E-14||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:GAT2(YMR136W)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Protein containing GATA family zinc finger motifs; similar to Gln3p and Dal80p; expression repressed by leucine Gene:GCN2(YDR283C)|FD-Score:10.6|P-value:2.02E-26||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN3(YKR026C)|FD-Score:5.29|P-value:6.17E-8||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:10.5|P-value:3.11E-26||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:HCH1(YNL281W)|FD-Score:3.15|P-value:8.26E-4||SGD DESC:Heat shock protein regulator; binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; GFP-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress Gene:HFA1(YMR207C)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:Mitochondrial acetyl-coenzyme A carboxylase; catalyzes the production of malonyl-CoA in mitochondrial fatty acid biosynthesis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:IML3(YBR107C)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Outer kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1 Gene:IST1(YNL265C)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p Gene:LAP2(YNL045W)|FD-Score:-3.8|P-value:7.33E-5||SGD DESC:Leucyl aminopeptidase yscIV (leukotriene A4 hydrolase) with epoxide hydrolase activity, metalloenzyme containing one zinc atom; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:LYS9(YNR050C)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Saccharopine dehydrogenase (NADP+, L-glutamate-forming); catalyzes the formation of saccharopine from alpha-aminoadipate 6-semialdehyde, the seventh step in lysine biosynthesis pathway; exhibits genetic and physical interactions with TRM112 Gene:MET10(YFR030W)|FD-Score:3.92|P-value:4.48E-5||SGD DESC:Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide Gene:MET16(YPR167C)|FD-Score:-3.17|P-value:7.59E-4||SGD DESC:3'-phosphoadenylsulfate reductase, reduces 3'-phosphoadenylyl sulfate to adenosine-3',5'-bisphosphate and free sulfite using reduced thioredoxin as cosubstrate, involved in sulfate assimilation and methionine metabolism Gene:NTH2(YBR001C)|FD-Score:3.19|P-value:7.03E-4||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:ORT1(YOR130C)|FD-Score:4.75|P-value:9.94E-7||SGD DESC:Ornithine transporter of the mitochondrial inner membrane, exports ornithine from mitochondria as part of arginine biosynthesis; human ortholog is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome Gene:OSH6(YKR003W)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Member of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion Gene:PGD1(YGL025C)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PHO88(YBR106W)|FD-Score:6.52|P-value:3.54E-11||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PRY2(YKR013W)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication Gene:RAI1(YGL246C)|FD-Score:3.73|P-value:9.67E-5||SGD DESC:Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z Gene:RPN4(YDL020C)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SMM1(YNR015W)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Dihydrouridine synthase, member of a family of dihydrouridine synthases including Dus1p, Smm1p, Dus3p, and Dus4p; modifies uridine residues at position 20 of cytoplasmic tRNAs Gene:SPC2(YML055W)|FD-Score:3.15|P-value:8.11E-4||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC25 Gene:SPG3(YDR504C)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources Gene:SPT4(YGR063C)|FD-Score:3.19|P-value:7.07E-4||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:STO1(YMR125W)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:TDH3(YGR192C)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; TDH3 has a paralog, TDH2, that arose from the whole genome duplication Gene:TLG2(YOL018C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TRP1(YDR007W)|FD-Score:5.92|P-value:1.58E-9||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:4.93|P-value:4.14E-7||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:5.5|P-value:1.86E-8||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:3.99|P-value:3.32E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:VPS36(YLR417W)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:YBL059W(YBL059W_p)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication Gene:YBR138C(YBR138C_p)|FD-Score:3.21|P-value:6.70E-4||SGD DESC:Cytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:7.48|P-value:3.83E-14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER079W(YER079W_p)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:Putative protein of unknown function Gene:YGL117W(YGL117W_p)|FD-Score:14.7|P-value:5.96E-49||SGD DESC:Putative protein of unknown function Gene:YGR153W(YGR153W_p)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Putative protein of unknown function Gene:YIL046W-A(YIL046W-A_p)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YJL045W(YJL045W)|FD-Score:4.02|P-value:2.93E-5||SGD DESC:Minor succinate dehydrogenase isozyme; homologous to Sdh1p, the major isozyme reponsible for the oxidation of succinate and transfer of electrons to ubiquinone; induced during the diauxic shift in a Cat8p-dependent manner Gene:YJL067W(YJL067W_d)|FD-Score:-3.14|P-value:8.54E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML108W(YML108W_p)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:Protein of unknown function; structure defines a new subfamily of the split beta-alpha-beta sandwiches; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YML108W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress Gene:YMR155W(YMR155W_p)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen Gene:YNL108C(YNL108C_p)|FD-Score:5.36|P-value:4.15E-8||SGD DESC:Putative protein of unknown function; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication Gene:YPL197C(YPL197C_d)|FD-Score:-3.86|P-value:5.73E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the ribosomal gene RPB7B Gene:YPR127W(YPR127W)|FD-Score:6.8|P-value:5.27E-12||SGD DESC:Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YPT7(YML001W)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7 Gene:ARG3(YJL088W)|FD-Score:5.47|P-value:2.27E-8||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ARO1(YDR127W)|FD-Score:4.35|P-value:6.78E-6||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:8.55|P-value:6.42E-18||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:BTS1(YPL069C)|FD-Score:-3.35|P-value:4.05E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CWP2(YKL096W-A)|FD-Score:3.23|P-value:6.09E-4||SGD DESC:Covalently linked cell wall mannoprotein, major constituent of the cell wall; plays a role in stabilizing the cell wall; involved in low pH resistance; precursor is GPI-anchored Gene:DYN2(YDR424C)|FD-Score:3.82|P-value:6.75E-5||SGD DESC:Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex Gene:ERG2(YMR202W)|FD-Score:7.44|P-value:5.06E-14||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:GAT2(YMR136W)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Protein containing GATA family zinc finger motifs; similar to Gln3p and Dal80p; expression repressed by leucine Gene:GCN2(YDR283C)|FD-Score:10.6|P-value:2.02E-26||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN3(YKR026C)|FD-Score:5.29|P-value:6.17E-8||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:10.5|P-value:3.11E-26||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:HCH1(YNL281W)|FD-Score:3.15|P-value:8.26E-4||SGD DESC:Heat shock protein regulator; binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; GFP-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress Gene:HFA1(YMR207C)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:Mitochondrial acetyl-coenzyme A carboxylase; catalyzes the production of malonyl-CoA in mitochondrial fatty acid biosynthesis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:IML3(YBR107C)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Outer kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1 Gene:IST1(YNL265C)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p Gene:LAP2(YNL045W)|FD-Score:-3.8|P-value:7.33E-5||SGD DESC:Leucyl aminopeptidase yscIV (leukotriene A4 hydrolase) with epoxide hydrolase activity, metalloenzyme containing one zinc atom; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:LYS9(YNR050C)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Saccharopine dehydrogenase (NADP+, L-glutamate-forming); catalyzes the formation of saccharopine from alpha-aminoadipate 6-semialdehyde, the seventh step in lysine biosynthesis pathway; exhibits genetic and physical interactions with TRM112 Gene:MET10(YFR030W)|FD-Score:3.92|P-value:4.48E-5||SGD DESC:Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide Gene:MET16(YPR167C)|FD-Score:-3.17|P-value:7.59E-4||SGD DESC:3'-phosphoadenylsulfate reductase, reduces 3'-phosphoadenylyl sulfate to adenosine-3',5'-bisphosphate and free sulfite using reduced thioredoxin as cosubstrate, involved in sulfate assimilation and methionine metabolism Gene:NTH2(YBR001C)|FD-Score:3.19|P-value:7.03E-4||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:ORT1(YOR130C)|FD-Score:4.75|P-value:9.94E-7||SGD DESC:Ornithine transporter of the mitochondrial inner membrane, exports ornithine from mitochondria as part of arginine biosynthesis; human ortholog is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome Gene:OSH6(YKR003W)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Member of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion Gene:PGD1(YGL025C)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PHO88(YBR106W)|FD-Score:6.52|P-value:3.54E-11||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PRY2(YKR013W)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication Gene:RAI1(YGL246C)|FD-Score:3.73|P-value:9.67E-5||SGD DESC:Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z Gene:RPN4(YDL020C)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SMM1(YNR015W)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Dihydrouridine synthase, member of a family of dihydrouridine synthases including Dus1p, Smm1p, Dus3p, and Dus4p; modifies uridine residues at position 20 of cytoplasmic tRNAs Gene:SPC2(YML055W)|FD-Score:3.15|P-value:8.11E-4||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC25 Gene:SPG3(YDR504C)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources Gene:SPT4(YGR063C)|FD-Score:3.19|P-value:7.07E-4||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:STO1(YMR125W)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:TDH3(YGR192C)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; TDH3 has a paralog, TDH2, that arose from the whole genome duplication Gene:TLG2(YOL018C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TRP1(YDR007W)|FD-Score:5.92|P-value:1.58E-9||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:4.93|P-value:4.14E-7||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:5.5|P-value:1.86E-8||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:3.99|P-value:3.32E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:VPS36(YLR417W)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:YBL059W(YBL059W_p)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication Gene:YBR138C(YBR138C_p)|FD-Score:3.21|P-value:6.70E-4||SGD DESC:Cytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:7.48|P-value:3.83E-14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER079W(YER079W_p)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:Putative protein of unknown function Gene:YGL117W(YGL117W_p)|FD-Score:14.7|P-value:5.96E-49||SGD DESC:Putative protein of unknown function Gene:YGR153W(YGR153W_p)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Putative protein of unknown function Gene:YIL046W-A(YIL046W-A_p)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YJL045W(YJL045W)|FD-Score:4.02|P-value:2.93E-5||SGD DESC:Minor succinate dehydrogenase isozyme; homologous to Sdh1p, the major isozyme reponsible for the oxidation of succinate and transfer of electrons to ubiquinone; induced during the diauxic shift in a Cat8p-dependent manner Gene:YJL067W(YJL067W_d)|FD-Score:-3.14|P-value:8.54E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML108W(YML108W_p)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:Protein of unknown function; structure defines a new subfamily of the split beta-alpha-beta sandwiches; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YML108W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress Gene:YMR155W(YMR155W_p)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen Gene:YNL108C(YNL108C_p)|FD-Score:5.36|P-value:4.15E-8||SGD DESC:Putative protein of unknown function; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication Gene:YPL197C(YPL197C_d)|FD-Score:-3.86|P-value:5.73E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the ribosomal gene RPB7B Gene:YPR127W(YPR127W)|FD-Score:6.8|P-value:5.27E-12||SGD DESC:Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YPT7(YML001W)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YNR035C8.141.93E-162.72ARC35Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin
YHR062C5.423.01E-80.46RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YHR069C4.963.48E-70.63RRP4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2)
YKL180W4.337.46E-60.52RPL17ARibosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
YGL102C_d3.816.90E-50.07YGL102C_dDubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential RPL28 gene encoding a large subunit ribosomal protein
YJL076W3.749.07E-50.03NET1Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication
YER133W3.711.02E-40.01GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YLR009W3.711.04E-40.33RLP24Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
YOR102W_d3.383.58E-40.06YOR102W_dDubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex
YER147C3.334.39E-40.04SCC4Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during double-strand break repair via phosphorylated histone H2AX
YOR224C3.295.05E-40.08RPB8RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III
YJR042W3.216.71E-40.03NUP85Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75
YPR133C3.187.31E-40.04SPN1Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype
YGR095C3.148.39E-40.01RRP46Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5)
YOL120C3.138.75E-40.05RPL18ARibosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL117W_p14.705.96E-49YGL117W_pPutative protein of unknown function
YDR283C10.602.02E-26GCN2Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control
YEL009C10.503.11E-26GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
YDR035W8.556.42E-18ARO33-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan
YDR008C_d7.483.83E-14YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YMR202W7.445.06E-14ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YPR127W6.805.27E-12YPR127WPutative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
YBR106W6.523.54E-11PHO88Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations
YDR007W5.921.58E-9TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YKL211C5.501.86E-8TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YJL088W5.472.27E-8ARG3Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine
YNL108C_p5.364.15E-8YNL108C_pPutative protein of unknown function; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication
YKR026C5.296.17E-8GCN3Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression
YER090W4.934.14E-7TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YOR130C4.759.94E-7ORT1Ornithine transporter of the mitochondrial inner membrane, exports ornithine from mitochondria as part of arginine biosynthesis; human ortholog is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome

GO enrichment analysis for SGTC_285
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.4890SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.0533333
0.4820SGTC_1801486-1293 102.8 μMChemDiv (Drug-like library)41405360.618182
0.4521.40E-295SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.0555556
0.4462.82E-287SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.0533333
0.4461.03E-286SGTC_2051486-1330 71.9 μMChemDiv (Drug-like library)41188340.755102
0.4103.34E-238SGTC_465cgp-37157 41.0 μMICCB bioactive library26880.112676
0.3961.05E-220SGTC_18825615643 20.0 μMMiscellaneous22530750.1375TRP & mitochondrial translation
0.3947.83E-219SGTC_1761486-1328 72.8 μMChemDiv (Drug-like library)223102310.709091
0.3862.93E-209SGTC_18815652484 16.0 μMMiscellaneous22551290.111111TRP & mitochondrial translation
0.3771.27E-198SGTC_6770929-0063 136.0 μMChemDiv (Drug-like library)15395830.0714286
0.3731.49E-194SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.0394737
0.3701.57E-190SGTC_2669eugenol 100.0 μMMicrosource (Natural product library)33140.0615385
0.3691.74E-189SGTC_23849036245 200.0 μMChembridge (Fragment library)64627660.104478
0.3681.45E-188SGTC_2524avocatin b 10.6 μMMicrosource (Natural product library)6710748NA
0.3671.51E-187SGTC_6360kpi-0074 126.0 μMChemDiv (Drug-like library)6164340.030303

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_13371486-101140.3 μM0.888889X1337ChemDiv (Drug-like library)443.903314.8142
SGTC_13391486-132122.7 μM0.8888893469382ChemDiv (Drug-like library)443.903324.8124ERG2
SGTC_11901486-133213 μM0.8039224150046ChemDiv (Drug-like library)487.955885.14325
SGTC_2051486-133071.9 μM0.7551024118834ChemDiv (Drug-like library)485.983066.26924
SGTC_1941486-132356.9 μM0.745178754ChemDiv (Drug-like library)478.348385.47524
SGTC_1761486-132872.83 μM0.70909122310231ChemDiv (Drug-like library)511.901295.75327
SGTC_5831488-109188.9 μM0.6382984303273ChemDiv (Drug-like library)501.575224.69326
SGTC_1801486-1293102.78 μM0.6181824140536ChemDiv (Drug-like library)491.482815.57427
SGTC_9811488-109454.9 μM0.5333333449522ChemDiv (Drug-like library)489.564523.20327
SGTC_13411486-1360280 μM0.5208333976547ChemDiv (Drug-like library)285.31950.99324