9024559

methyl 2-morpholin-4-yl-5-(pyridine-3-carbonylamino)benzoate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2854
Screen concentration 71.4 μM
Source Chembridge (Drug-like library)
PubChem CID 3163529
SMILES COC(=O)C1=C(C=CC(=C1)NC(=O)C2=CN=CC=C2)N3CCOCC3
Standardized SMILES COC(=O)c1cc(NC(=O)c2cccnc2)ccc1N3CCOCC3
Molecular weight 341.3612
ALogP 1.17
H-bond donor count 1
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 4.05
% growth inhibition (Hom. pool) -0.02


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3163529
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:8.59|P-value:4.28E-18|Clearance:2.51||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ARP7(YPR034W)|FD-Score:-3.64|P-value:1.36E-4|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CDC24(YAL041W)|FD-Score:-3.92|P-value:4.35E-5|Clearance:0||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:DRE2(YKR071C)|FD-Score:-3.26|P-value:5.65E-4|Clearance:0||SGD DESC:Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress Gene:FCP1(YMR277W)|FD-Score:-3.47|P-value:2.64E-4|Clearance:0||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:LSM5(YER146W)|FD-Score:3.54|P-value:2.01E-4|Clearance:0.04||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MED4(YOR174W)|FD-Score:3.5|P-value:2.32E-4|Clearance:0.15||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MEX67(YPL169C)|FD-Score:3.35|P-value:4.07E-4|Clearance:0.04||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:MGE1(YOR232W)|FD-Score:3.18|P-value:7.33E-4|Clearance:0.04||SGD DESC:Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE Gene:NIC96(YFR002W)|FD-Score:3.21|P-value:6.69E-4|Clearance:0.01||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:RPB3(YIL021W)|FD-Score:-4|P-value:3.15E-5|Clearance:0||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPB8(YOR224C)|FD-Score:-6.29|P-value:1.62E-10|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPC25(YKL144C)|FD-Score:-3.95|P-value:3.85E-5|Clearance:0||SGD DESC:RNA polymerase III subunit C25, required for transcription initiation; forms a heterodimer with Rpc17p; paralog of Rpb7p Gene:RPC53(YDL150W)|FD-Score:-4.69|P-value:1.34E-6|Clearance:0||SGD DESC:RNA polymerase III subunit C53 Gene:RPP0(YLR340W)|FD-Score:3.26|P-value:5.65E-4|Clearance:0.05||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:RPP1(YHR062C)|FD-Score:-5.55|P-value:1.45E-8|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS13(YDR064W)|FD-Score:9.94|P-value:1.36E-23|Clearance:2.51||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:RPS3(YNL178W)|FD-Score:3.7|P-value:1.09E-4|Clearance:0.16||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RRP4(YHR069C)|FD-Score:3.77|P-value:8.23E-5|Clearance:0.07||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:RRP7(YCL031C)|FD-Score:-3.33|P-value:4.35E-4|Clearance:0||SGD DESC:Essential protein involved in rRNA processing and ribosome biogenesis; protein abundance increases in response to DNA replication stress Gene:RRP9(YPR137W)|FD-Score:3.11|P-value:9.28E-4|Clearance:0.03||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:RSC9(YML127W)|FD-Score:-3.15|P-value:8.17E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEN15(YMR059W)|FD-Score:3.86|P-value:5.61E-5|Clearance:0.01||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p Gene:SRP102(YKL154W)|FD-Score:3.2|P-value:6.82E-4|Clearance:0.02||SGD DESC:Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane Gene:SUA5(YGL169W)|FD-Score:-4.63|P-value:1.82E-6|Clearance:0||SGD DESC:Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family Gene:TAF6(YGL112C)|FD-Score:6.09|P-value:5.79E-10|Clearance:1.39||SGD DESC:Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4 Gene:TIF35(YDR429C)|FD-Score:-4.46|P-value:4.13E-6|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TRE2(YOR256C)|FD-Score:-3.92|P-value:4.36E-5|Clearance:0||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:UGP1(YKL035W)|FD-Score:4.56|P-value:2.62E-6|Clearance:0.69||SGD DESC:UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication Gene:YDL152W(YDL152W_d)|FD-Score:-3.79|P-value:7.49E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YGR114C(YGR114C_d)|FD-Score:4.7|P-value:1.31E-6|Clearance:0.14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YGR265W(YGR265W_d)|FD-Score:-3.34|P-value:4.13E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase Gene:YJL086C(YJL086C_d)|FD-Score:3.3|P-value:4.76E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YLR076C(YLR076C_d)|FD-Score:3.15|P-value:8.29E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:YPR142C(YPR142C_d)|FD-Score:3.85|P-value:5.83E-5|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing Gene:ACT1(YFL039C)|FD-Score:8.59|P-value:4.28E-18|Clearance:2.51||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ARP7(YPR034W)|FD-Score:-3.64|P-value:1.36E-4|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CDC24(YAL041W)|FD-Score:-3.92|P-value:4.35E-5|Clearance:0||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:DRE2(YKR071C)|FD-Score:-3.26|P-value:5.65E-4|Clearance:0||SGD DESC:Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress Gene:FCP1(YMR277W)|FD-Score:-3.47|P-value:2.64E-4|Clearance:0||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:LSM5(YER146W)|FD-Score:3.54|P-value:2.01E-4|Clearance:0.04||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MED4(YOR174W)|FD-Score:3.5|P-value:2.32E-4|Clearance:0.15||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MEX67(YPL169C)|FD-Score:3.35|P-value:4.07E-4|Clearance:0.04||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:MGE1(YOR232W)|FD-Score:3.18|P-value:7.33E-4|Clearance:0.04||SGD DESC:Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE Gene:NIC96(YFR002W)|FD-Score:3.21|P-value:6.69E-4|Clearance:0.01||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:RPB3(YIL021W)|FD-Score:-4|P-value:3.15E-5|Clearance:0||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPB8(YOR224C)|FD-Score:-6.29|P-value:1.62E-10|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPC25(YKL144C)|FD-Score:-3.95|P-value:3.85E-5|Clearance:0||SGD DESC:RNA polymerase III subunit C25, required for transcription initiation; forms a heterodimer with Rpc17p; paralog of Rpb7p Gene:RPC53(YDL150W)|FD-Score:-4.69|P-value:1.34E-6|Clearance:0||SGD DESC:RNA polymerase III subunit C53 Gene:RPP0(YLR340W)|FD-Score:3.26|P-value:5.65E-4|Clearance:0.05||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:RPP1(YHR062C)|FD-Score:-5.55|P-value:1.45E-8|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS13(YDR064W)|FD-Score:9.94|P-value:1.36E-23|Clearance:2.51||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:RPS3(YNL178W)|FD-Score:3.7|P-value:1.09E-4|Clearance:0.16||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RRP4(YHR069C)|FD-Score:3.77|P-value:8.23E-5|Clearance:0.07||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:RRP7(YCL031C)|FD-Score:-3.33|P-value:4.35E-4|Clearance:0||SGD DESC:Essential protein involved in rRNA processing and ribosome biogenesis; protein abundance increases in response to DNA replication stress Gene:RRP9(YPR137W)|FD-Score:3.11|P-value:9.28E-4|Clearance:0.03||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:RSC9(YML127W)|FD-Score:-3.15|P-value:8.17E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEN15(YMR059W)|FD-Score:3.86|P-value:5.61E-5|Clearance:0.01||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p Gene:SRP102(YKL154W)|FD-Score:3.2|P-value:6.82E-4|Clearance:0.02||SGD DESC:Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane Gene:SUA5(YGL169W)|FD-Score:-4.63|P-value:1.82E-6|Clearance:0||SGD DESC:Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family Gene:TAF6(YGL112C)|FD-Score:6.09|P-value:5.79E-10|Clearance:1.39||SGD DESC:Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4 Gene:TIF35(YDR429C)|FD-Score:-4.46|P-value:4.13E-6|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TRE2(YOR256C)|FD-Score:-3.92|P-value:4.36E-5|Clearance:0||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:UGP1(YKL035W)|FD-Score:4.56|P-value:2.62E-6|Clearance:0.69||SGD DESC:UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication Gene:YDL152W(YDL152W_d)|FD-Score:-3.79|P-value:7.49E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YGR114C(YGR114C_d)|FD-Score:4.7|P-value:1.31E-6|Clearance:0.14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YGR265W(YGR265W_d)|FD-Score:-3.34|P-value:4.13E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase Gene:YJL086C(YJL086C_d)|FD-Score:3.3|P-value:4.76E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YLR076C(YLR076C_d)|FD-Score:3.15|P-value:8.29E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:YPR142C(YPR142C_d)|FD-Score:3.85|P-value:5.83E-5|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3163529
Download HOP data (tab-delimited text)  (excel)
Gene:ADO1(YJR105W)|FD-Score:-4.19|P-value:1.38E-5||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AEP2(YMR282C)|FD-Score:5.58|P-value:1.22E-8||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AFG3(YER017C)|FD-Score:3.88|P-value:5.13E-5||SGD DESC:Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging Gene:AFT1(YGL071W)|FD-Score:-5.25|P-value:7.42E-8||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ALD6(YPL061W)|FD-Score:4.16|P-value:1.56E-5||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:ARO1(YDR127W)|FD-Score:3.12|P-value:9.12E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATC1(YDR184C)|FD-Score:5.74|P-value:4.86E-9||SGD DESC:Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress Gene:BNI4(YNL233W)|FD-Score:4.28|P-value:9.54E-6||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:BUD21(YOR078W)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern Gene:CDA1(YLR307W)|FD-Score:5.75|P-value:4.37E-9||SGD DESC:Chitin deacetylase, together with Cda2p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall Gene:CMR3(YPR013C_p)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Putative zinc finger protein; YPR013C is not an essential gene Gene:COG6(YNL041C)|FD-Score:-3.34|P-value:4.16E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CPS1(YJL172W)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Vacuolar carboxypeptidase yscS; expression is induced under low-nitrogen conditions Gene:CTF8(YHR191C)|FD-Score:-4.14|P-value:1.73E-5||SGD DESC:Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion Gene:DIA1(YMR316W)|FD-Score:-3.57|P-value:1.76E-4||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DUG2(YBR281C)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:FCY22(YER060W-A)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:GEX2(YKR106W)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Proton:glutathione antiporter localized to the vacuolar and plasma membranes; expressed at a very low level; almost identical to paralog Gex1p; potential role in resistance to oxidative stress and modulation of the PKA pathway Gene:GOS1(YHL031C)|FD-Score:-4.88|P-value:5.21E-7||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:GUP2(YPL189W)|FD-Score:3.97|P-value:3.53E-5||SGD DESC:Probable membrane protein with a possible role in proton symport of glycerol; member of the MBOAT family of putative membrane-bound O-acyltransferases; Gup1p homolog Gene:HMS1(YOR032C)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Basic helix-loop-helix (bHLH) protein with similarity to myc-family transcription factors; overexpression confers hyperfilamentous growth and suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant Gene:HTZ1(YOL012C)|FD-Score:3.79|P-value:7.49E-5||SGD DESC:Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin Gene:HXT2(YMR011W)|FD-Score:4.58|P-value:2.30E-6||SGD DESC:High-affinity glucose transporter of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:ILV6(YCL009C)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria Gene:INM2(YDR287W)|FD-Score:3.8|P-value:7.32E-5||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol; enzymatic activity requires magnesium ions and is inhibited by lithium and sodium ions; inm1 inm2 double mutant lacks inositol auxotrophy Gene:ISC10(YER180C)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:KCC4(YCL024W)|FD-Score:-3.4|P-value:3.31E-4||SGD DESC:Protein kinase of the bud neck involved in the septin checkpoint; associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p; KCC4 has a paralog, GIN4, that arose from the whole genome duplication Gene:KEL2(YGR238C)|FD-Score:3.12|P-value:9.12E-4||SGD DESC:Protein that functions in a complex with Kel1p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:LCB4(YOR171C)|FD-Score:3.89|P-value:4.99E-5||SGD DESC:Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes Gene:LEM3(YNL323W)|FD-Score:-8.74|P-value:1.12E-18||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MCM22(YJR135C)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Outer kinetochore protein and component of the Ctf3 subcomplex; binds to centromeric DNA in a Ctf19p-dependent manner; involved in chromosome segregation and minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-K and fission yeast sim4 Gene:MRK1(YDL079C)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation Gene:NAM2(YLR382C)|FD-Score:4.76|P-value:9.66E-7||SGD DESC:Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Gene:OPT1(YJL212C)|FD-Score:5.67|P-value:7.35E-9||SGD DESC:Proton-coupled oligopeptide transporter of the plasma membrane; also transports glutathione and phytochelatin; member of the OPT family Gene:PDC1(YLR044C)|FD-Score:3.12|P-value:8.96E-4||SGD DESC:Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism Gene:PEP12(YOR036W)|FD-Score:-3.85|P-value:6.00E-5||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PET117(YER058W)|FD-Score:-3.37|P-value:3.77E-4||SGD DESC:Protein required for assembly of cytochrome c oxidase Gene:PEX32(YBR168W)|FD-Score:-4.5|P-value:3.38E-6||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PHM6(YDR281C)|FD-Score:-4.7|P-value:1.28E-6||SGD DESC:Protein of unknown function, expression is regulated by phosphate levels Gene:PRM1(YNL279W)|FD-Score:-3.09|P-value:9.86E-4||SGD DESC:Pheromone-regulated multispanning membrane protein involved in membrane fusion during mating; predicted to have 5 transmembrane segments and a coiled coil domain; localizes to the shmoo tip; regulated by Ste12p Gene:PSR1(YLL010C)|FD-Score:3.18|P-value:7.32E-4||SGD DESC:Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:PTK1(YKL198C)|FD-Score:4.05|P-value:2.61E-5||SGD DESC:Putative serine/threonine protein kinase that regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein Gene:RCN1(YKL159C)|FD-Score:-3.57|P-value:1.75E-4||SGD DESC:Protein involved in calcineurin regulation during calcium signaling; has similarity to H. sapiens DSCR1 which is found in the Down Syndrome candidate region Gene:REC8(YPR007C)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Meiosis-specific component of sister chromatid cohesion complex; maintains cohesion between sister chromatids during meiosis I; maintains cohesion between centromeres of sister chromatids until meiosis II; homolog of S. pombe Rec8p Gene:RGA1(YOR127W)|FD-Score:3.96|P-value:3.79E-5||SGD DESC:GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RIM15(YFL033C)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase Gene:RKM3(YBR030W)|FD-Score:-4.12|P-value:1.91E-5||SGD DESC:Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 40); nuclear SET domain containing protein Gene:ROM2(YLR371W)|FD-Score:-4.59|P-value:2.26E-6||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL23B(YER117W)|FD-Score:3.87|P-value:5.47E-5||SGD DESC:Ribosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication Gene:RPL26B(YGR034W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication Gene:RPL31B(YLR406C)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Ribosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:-3.67|P-value:1.20E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS0A(YGR214W)|FD-Score:3.25|P-value:5.86E-4||SGD DESC:Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication; Gene:SCP1(YOR367W)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin Gene:SHE9(YDR393W)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Mitochondrial inner membrane protein required for normal mitochondrial morphology, may be involved in fission of the inner membrane; forms a homo-oligomeric complex Gene:SHP1(YBL058W)|FD-Score:-3.98|P-value:3.49E-5||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein that regulates Glc7p phosphatase activity and interacts with Cdc48p; interacts with ubiquitylated proteins in vivo and is required for degradation of a ubiquitylated model substrate Gene:SLX8(YER116C)|FD-Score:3.95|P-value:3.92E-5||SGD DESC:Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress Gene:SPC72(YAL047C)|FD-Score:-4.03|P-value:2.82E-5||SGD DESC:Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization Gene:SPT7(YBR081C)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex Gene:SRX1(YKL086W)|FD-Score:4.81|P-value:7.41E-7||SGD DESC:Sulfiredoxin; contributes to oxidative stress resistance by reducing cysteine-sulfinic acid groups in the peroxiredoxin Tsa1p, which is formed upon exposure to oxidants; conserved in higher eukaryotes; protein abundance increases in response to DNA replication stress Gene:SSP2(YOR242C)|FD-Score:3.11|P-value:9.51E-4||SGD DESC:Sporulation specific protein that localizes to the spore wall; required for sporulation at a point after meiosis II and during spore wall formation; SSP2 expression is induced midway in meiosis Gene:SVL3(YPL032C)|FD-Score:6.8|P-value:5.35E-12||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:TDP1(YBR223C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Tyrosyl-DNA phosphodiesterase I; hydrolyzes 3' and 5'-phosphotyrosyl bonds; involved in the repair of DNA lesions created by topoisomerase I and topoisomerase II; mutations in human homolog result in the neurodegenerative disease SCANI Gene:TEP1(YNL128W)|FD-Score:-3.23|P-value:6.26E-4||SGD DESC:PTEN homolog with no demonstrated inositol lipid phosphatase activity; plays a role in normal sporulation; homolog of human tumor suppressor gene PTEN/MMAC1/TEP1 and fission yeast ptn1 Gene:TIF4632(YGL049C)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); TIF4632 has a paralog, TIF4631, that arose from the whole genome duplication Gene:TKL1(YPR074C)|FD-Score:3.15|P-value:8.03E-4||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:TOP1(YOL006C)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination Gene:TRP2(YER090W)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TSR2(YLR435W)|FD-Score:4.29|P-value:8.76E-6||SGD DESC:Protein with a potential role in pre-rRNA processing Gene:TUM1(YOR251C)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Rhodanese domain sulfur transferase, accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; may be mitochondrially localized Gene:UFD2(YDL190C)|FD-Score:3.75|P-value:8.99E-5||SGD DESC:Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3 Gene:VPS24(YKL041W)|FD-Score:4.28|P-value:9.23E-6||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:VPS27(YNR006W)|FD-Score:4.29|P-value:8.86E-6||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS63(YLR261C_d)|FD-Score:-4.71|P-value:1.22E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:VPS68(YOL129W)|FD-Score:5.09|P-value:1.76E-7||SGD DESC:Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria Gene:YBR196C-A(YBR196C-A_p)|FD-Score:-11.6|P-value:3.29E-31||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBR232C(YBR232C_d)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR157W(YDR157W_d)|FD-Score:3.86|P-value:5.63E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR203W(YDR203W_d)|FD-Score:-4.2|P-value:1.35E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR433W(YDR433W_d)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR514C(YDR514C)|FD-Score:-3.89|P-value:4.93E-5||SGD DESC:Protein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication Gene:YER097W(YER097W_d)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR122C-A(YGR122C-A_d)|FD-Score:3.88|P-value:5.25E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, similar to YLR334C and YOL106W Gene:YIL012W(YIL012W_d)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLL047W(YLL047W_d)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RNP1 Gene:YLR264C-A(YLR264C-A_p)|FD-Score:3.37|P-value:3.69E-4||SGD DESC:Putative protein of unknown function Gene:YLR285C-A(YLR285C-A_p)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YLR460C(YLR460C_p)|FD-Score:7.65|P-value:1.01E-14||SGD DESC:Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine Gene:YML083C(YML083C_p)|FD-Score:-3.24|P-value:6.03E-4||SGD DESC:Protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions Gene:YMR153C-A(YMR153C-A_d)|FD-Score:-4.69|P-value:1.39E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NUP53 Gene:YNL146W(YNL146W_p)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNL146W is not an essential gene Gene:YOR385W(YOR385W_p)|FD-Score:-3.77|P-value:8.01E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:YPL077C(YPL077C_p)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication Gene:YPT53(YNL093W)|FD-Score:7.09|P-value:6.94E-13||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication Gene:ADO1(YJR105W)|FD-Score:-4.19|P-value:1.38E-5||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AEP2(YMR282C)|FD-Score:5.58|P-value:1.22E-8||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AFG3(YER017C)|FD-Score:3.88|P-value:5.13E-5||SGD DESC:Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging Gene:AFT1(YGL071W)|FD-Score:-5.25|P-value:7.42E-8||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ALD6(YPL061W)|FD-Score:4.16|P-value:1.56E-5||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:ARO1(YDR127W)|FD-Score:3.12|P-value:9.12E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATC1(YDR184C)|FD-Score:5.74|P-value:4.86E-9||SGD DESC:Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress Gene:BNI4(YNL233W)|FD-Score:4.28|P-value:9.54E-6||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:BUD21(YOR078W)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern Gene:CDA1(YLR307W)|FD-Score:5.75|P-value:4.37E-9||SGD DESC:Chitin deacetylase, together with Cda2p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall Gene:CMR3(YPR013C_p)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Putative zinc finger protein; YPR013C is not an essential gene Gene:COG6(YNL041C)|FD-Score:-3.34|P-value:4.16E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CPS1(YJL172W)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Vacuolar carboxypeptidase yscS; expression is induced under low-nitrogen conditions Gene:CTF8(YHR191C)|FD-Score:-4.14|P-value:1.73E-5||SGD DESC:Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion Gene:DIA1(YMR316W)|FD-Score:-3.57|P-value:1.76E-4||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DUG2(YBR281C)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:FCY22(YER060W-A)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:GEX2(YKR106W)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Proton:glutathione antiporter localized to the vacuolar and plasma membranes; expressed at a very low level; almost identical to paralog Gex1p; potential role in resistance to oxidative stress and modulation of the PKA pathway Gene:GOS1(YHL031C)|FD-Score:-4.88|P-value:5.21E-7||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:GUP2(YPL189W)|FD-Score:3.97|P-value:3.53E-5||SGD DESC:Probable membrane protein with a possible role in proton symport of glycerol; member of the MBOAT family of putative membrane-bound O-acyltransferases; Gup1p homolog Gene:HMS1(YOR032C)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Basic helix-loop-helix (bHLH) protein with similarity to myc-family transcription factors; overexpression confers hyperfilamentous growth and suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant Gene:HTZ1(YOL012C)|FD-Score:3.79|P-value:7.49E-5||SGD DESC:Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin Gene:HXT2(YMR011W)|FD-Score:4.58|P-value:2.30E-6||SGD DESC:High-affinity glucose transporter of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:ILV6(YCL009C)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria Gene:INM2(YDR287W)|FD-Score:3.8|P-value:7.32E-5||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol; enzymatic activity requires magnesium ions and is inhibited by lithium and sodium ions; inm1 inm2 double mutant lacks inositol auxotrophy Gene:ISC10(YER180C)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:KCC4(YCL024W)|FD-Score:-3.4|P-value:3.31E-4||SGD DESC:Protein kinase of the bud neck involved in the septin checkpoint; associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p; KCC4 has a paralog, GIN4, that arose from the whole genome duplication Gene:KEL2(YGR238C)|FD-Score:3.12|P-value:9.12E-4||SGD DESC:Protein that functions in a complex with Kel1p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:LCB4(YOR171C)|FD-Score:3.89|P-value:4.99E-5||SGD DESC:Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes Gene:LEM3(YNL323W)|FD-Score:-8.74|P-value:1.12E-18||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MCM22(YJR135C)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Outer kinetochore protein and component of the Ctf3 subcomplex; binds to centromeric DNA in a Ctf19p-dependent manner; involved in chromosome segregation and minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-K and fission yeast sim4 Gene:MRK1(YDL079C)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation Gene:NAM2(YLR382C)|FD-Score:4.76|P-value:9.66E-7||SGD DESC:Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Gene:OPT1(YJL212C)|FD-Score:5.67|P-value:7.35E-9||SGD DESC:Proton-coupled oligopeptide transporter of the plasma membrane; also transports glutathione and phytochelatin; member of the OPT family Gene:PDC1(YLR044C)|FD-Score:3.12|P-value:8.96E-4||SGD DESC:Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism Gene:PEP12(YOR036W)|FD-Score:-3.85|P-value:6.00E-5||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PET117(YER058W)|FD-Score:-3.37|P-value:3.77E-4||SGD DESC:Protein required for assembly of cytochrome c oxidase Gene:PEX32(YBR168W)|FD-Score:-4.5|P-value:3.38E-6||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PHM6(YDR281C)|FD-Score:-4.7|P-value:1.28E-6||SGD DESC:Protein of unknown function, expression is regulated by phosphate levels Gene:PRM1(YNL279W)|FD-Score:-3.09|P-value:9.86E-4||SGD DESC:Pheromone-regulated multispanning membrane protein involved in membrane fusion during mating; predicted to have 5 transmembrane segments and a coiled coil domain; localizes to the shmoo tip; regulated by Ste12p Gene:PSR1(YLL010C)|FD-Score:3.18|P-value:7.32E-4||SGD DESC:Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:PTK1(YKL198C)|FD-Score:4.05|P-value:2.61E-5||SGD DESC:Putative serine/threonine protein kinase that regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein Gene:RCN1(YKL159C)|FD-Score:-3.57|P-value:1.75E-4||SGD DESC:Protein involved in calcineurin regulation during calcium signaling; has similarity to H. sapiens DSCR1 which is found in the Down Syndrome candidate region Gene:REC8(YPR007C)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Meiosis-specific component of sister chromatid cohesion complex; maintains cohesion between sister chromatids during meiosis I; maintains cohesion between centromeres of sister chromatids until meiosis II; homolog of S. pombe Rec8p Gene:RGA1(YOR127W)|FD-Score:3.96|P-value:3.79E-5||SGD DESC:GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RIM15(YFL033C)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase Gene:RKM3(YBR030W)|FD-Score:-4.12|P-value:1.91E-5||SGD DESC:Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 40); nuclear SET domain containing protein Gene:ROM2(YLR371W)|FD-Score:-4.59|P-value:2.26E-6||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL23B(YER117W)|FD-Score:3.87|P-value:5.47E-5||SGD DESC:Ribosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication Gene:RPL26B(YGR034W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication Gene:RPL31B(YLR406C)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Ribosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:-3.67|P-value:1.20E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS0A(YGR214W)|FD-Score:3.25|P-value:5.86E-4||SGD DESC:Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication; Gene:SCP1(YOR367W)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin Gene:SHE9(YDR393W)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Mitochondrial inner membrane protein required for normal mitochondrial morphology, may be involved in fission of the inner membrane; forms a homo-oligomeric complex Gene:SHP1(YBL058W)|FD-Score:-3.98|P-value:3.49E-5||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein that regulates Glc7p phosphatase activity and interacts with Cdc48p; interacts with ubiquitylated proteins in vivo and is required for degradation of a ubiquitylated model substrate Gene:SLX8(YER116C)|FD-Score:3.95|P-value:3.92E-5||SGD DESC:Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress Gene:SPC72(YAL047C)|FD-Score:-4.03|P-value:2.82E-5||SGD DESC:Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization Gene:SPT7(YBR081C)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex Gene:SRX1(YKL086W)|FD-Score:4.81|P-value:7.41E-7||SGD DESC:Sulfiredoxin; contributes to oxidative stress resistance by reducing cysteine-sulfinic acid groups in the peroxiredoxin Tsa1p, which is formed upon exposure to oxidants; conserved in higher eukaryotes; protein abundance increases in response to DNA replication stress Gene:SSP2(YOR242C)|FD-Score:3.11|P-value:9.51E-4||SGD DESC:Sporulation specific protein that localizes to the spore wall; required for sporulation at a point after meiosis II and during spore wall formation; SSP2 expression is induced midway in meiosis Gene:SVL3(YPL032C)|FD-Score:6.8|P-value:5.35E-12||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:TDP1(YBR223C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Tyrosyl-DNA phosphodiesterase I; hydrolyzes 3' and 5'-phosphotyrosyl bonds; involved in the repair of DNA lesions created by topoisomerase I and topoisomerase II; mutations in human homolog result in the neurodegenerative disease SCANI Gene:TEP1(YNL128W)|FD-Score:-3.23|P-value:6.26E-4||SGD DESC:PTEN homolog with no demonstrated inositol lipid phosphatase activity; plays a role in normal sporulation; homolog of human tumor suppressor gene PTEN/MMAC1/TEP1 and fission yeast ptn1 Gene:TIF4632(YGL049C)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); TIF4632 has a paralog, TIF4631, that arose from the whole genome duplication Gene:TKL1(YPR074C)|FD-Score:3.15|P-value:8.03E-4||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:TOP1(YOL006C)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination Gene:TRP2(YER090W)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TSR2(YLR435W)|FD-Score:4.29|P-value:8.76E-6||SGD DESC:Protein with a potential role in pre-rRNA processing Gene:TUM1(YOR251C)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Rhodanese domain sulfur transferase, accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; may be mitochondrially localized Gene:UFD2(YDL190C)|FD-Score:3.75|P-value:8.99E-5||SGD DESC:Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3 Gene:VPS24(YKL041W)|FD-Score:4.28|P-value:9.23E-6||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:VPS27(YNR006W)|FD-Score:4.29|P-value:8.86E-6||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS63(YLR261C_d)|FD-Score:-4.71|P-value:1.22E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:VPS68(YOL129W)|FD-Score:5.09|P-value:1.76E-7||SGD DESC:Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria Gene:YBR196C-A(YBR196C-A_p)|FD-Score:-11.6|P-value:3.29E-31||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBR232C(YBR232C_d)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR157W(YDR157W_d)|FD-Score:3.86|P-value:5.63E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR203W(YDR203W_d)|FD-Score:-4.2|P-value:1.35E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR433W(YDR433W_d)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR514C(YDR514C)|FD-Score:-3.89|P-value:4.93E-5||SGD DESC:Protein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication Gene:YER097W(YER097W_d)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR122C-A(YGR122C-A_d)|FD-Score:3.88|P-value:5.25E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, similar to YLR334C and YOL106W Gene:YIL012W(YIL012W_d)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLL047W(YLL047W_d)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RNP1 Gene:YLR264C-A(YLR264C-A_p)|FD-Score:3.37|P-value:3.69E-4||SGD DESC:Putative protein of unknown function Gene:YLR285C-A(YLR285C-A_p)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YLR460C(YLR460C_p)|FD-Score:7.65|P-value:1.01E-14||SGD DESC:Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine Gene:YML083C(YML083C_p)|FD-Score:-3.24|P-value:6.03E-4||SGD DESC:Protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions Gene:YMR153C-A(YMR153C-A_d)|FD-Score:-4.69|P-value:1.39E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NUP53 Gene:YNL146W(YNL146W_p)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNL146W is not an essential gene Gene:YOR385W(YOR385W_p)|FD-Score:-3.77|P-value:8.01E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:YPL077C(YPL077C_p)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication Gene:YPT53(YNL093W)|FD-Score:7.09|P-value:6.94E-13||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR064W9.941.36E-232.51RPS13Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15
YFL039C8.594.28E-182.51ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YGL112C6.095.79E-101.39TAF6Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4
YGR114C_d4.701.31E-60.14YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YKL035W4.562.62E-60.69UGP1UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication
YMR059W3.865.61E-50.01SEN15Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p
YPR142C_d3.855.83E-50.09YPR142C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing
YHR069C3.778.23E-50.07RRP4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2)
YNL178W3.701.09E-40.16RPS3Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3
YER146W3.542.01E-40.04LSM5Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA
YOR174W3.502.32E-40.15MED4Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YPL169C3.354.07E-40.04MEX67Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP
YJL086C_d3.304.76E-40.05YJL086C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1
YLR340W3.265.65E-40.05RPP0Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10
YFR002W3.216.69E-40.01NIC96Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p)

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR460C_p7.651.01E-14YLR460C_pMember of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine
YNL093W7.096.94E-13YPT53Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication
YPL032C6.805.35E-12SVL3Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress
YLR307W5.754.37E-9CDA1Chitin deacetylase, together with Cda2p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall
YDR184C5.744.86E-9ATC1Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress
YJL212C5.677.35E-9OPT1Proton-coupled oligopeptide transporter of the plasma membrane; also transports glutathione and phytochelatin; member of the OPT family
YMR282C5.581.22E-8AEP2Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader
YOL129W5.091.76E-7VPS68Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria
YKL086W4.817.41E-7SRX1Sulfiredoxin; contributes to oxidative stress resistance by reducing cysteine-sulfinic acid groups in the peroxiredoxin Tsa1p, which is formed upon exposure to oxidants; conserved in higher eukaryotes; protein abundance increases in response to DNA replication stress
YLR382C4.769.66E-7NAM2Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance
YMR011W4.582.30E-6HXT2High-affinity glucose transporter of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose
YLR435W4.298.76E-6TSR2Protein with a potential role in pre-rRNA processing
YNR006W4.298.86E-6VPS27Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p)
YKL041W4.289.23E-6VPS24One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway
YNL233W4.289.54E-6BNI4Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p

GO enrichment analysis for SGTC_2854
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1424.69E-28SGTC_501nsc-95397 161.0 μMICCB bioactive library2620930.0714286
0.1125.83E-18SGTC_1681tripelennamine 100.0 μMNIH Clinical Collection90660.0933333
0.0772.94E-9SGTC_28569022925 19.5 μMChembridge (Drug-like library)49448620.151899
0.0764.80E-9SGTC_23479049783 174.8 μMChembridge (Fragment library)64679880.191781
0.0699.23E-8SGTC_32939120939 62.0 μMChembridge (Drug-like library)9340870.107143
0.0672.13E-7SGTC_1649st011737 51.3 μMTimTec (Natural product derivative library)17610350.0941176
0.0663.49E-7SGTC_1715st032160 10.2 μMTimTec (Natural product derivative library)52898250.0851064
0.0631.31E-6SGTC_2620irigenin 100.0 μMMicrosource (Natural product library)54641700.0963855
0.0613.05E-6SGTC_12191469-0211 33.1 μMChemDiv (Drug-like library)68222820.127907
0.0613.20E-6SGTC_1636st007707 68.9 μMTimTec (Natural product derivative library)4030510.15
0.0603.59E-6SGTC_20155119600 184.0 μMChembridge (Fragment library)28287860.0909091
0.0596.36E-6SGTC_32599138485 49.5 μMChembridge (Drug-like library)176461530.115385
0.0571.16E-5SGTC_1764st044502 93.5 μMTimTec (Natural product derivative library)84070.101449
0.0571.25E-5SGTC_5154092-1064 102.0 μMChemDiv (Drug-like library)7173530.112676plasma membrane duress
0.0561.92E-5SGTC_2551apiole 73.9 μMMicrosource (Natural product library)106590.105263

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3283910933235.82 μM0.54687517027281Chembridge (Drug-like library)329.82393.45913DNA intercalators
SGTC_3034909042149.47 μM0.47692317173761Chembridge (Drug-like library)304.364142.381560S ribosome export
SGTC_3009908081971.43 μM0.437517027216Chembridge (Drug-like library)342.388982.43115
SGTC_2967908953849.85 μM0.421875728216Chembridge (Drug-like library)300.663633.0715
SGTC_3013908013371.43 μM0.417050193Chembridge (Drug-like library)354.442743.64214
SGTC_3010908008971.43 μM0.39130417027233Chembridge (Drug-like library)378.2387833.49714
SGTC_3032908942749.47 μM0.37517173707Chembridge (Drug-like library)318.390722.7291560S ribosome export
SGTC_9861493-048384 μM0.37096840079ChemDiv (Drug-like library)256.256681.31914
SGTC_3232913287449.47 μM0.3666673784940Chembridge (Drug-like library)255.268622.4713RPP1 & pyrimidine depletion
SGTC_5544483-2211187 μM0.365079747107ChemDiv (Drug-like library)285.29462.45314