9034755

N-[2-[(4-ethoxyphenyl)carbamoyl]phenyl]pyridine-4-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2864
Screen concentration 47.6 μM
Source Chembridge (Drug-like library)
PubChem CID 4930451
SMILES CCOC1=CC=C(C=C1)NC(=O)C2=CC=CC=C2NC(=O)C3=CC=NC=C3
Standardized SMILES CCOc1ccc(NC(=O)c2ccccc2NC(=O)c3ccncc3)cc1
Molecular weight 361.3939
ALogP 2.58
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.39
% growth inhibition (Hom. pool) -2.15


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4930451
Download HIP data (tab-delimited text)  (excel)
Gene:ARP9(YMR033W)|FD-Score:4.13|P-value:1.79E-5|Clearance:0.06||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:ECM16(YMR128W)|FD-Score:4.7|P-value:1.32E-6|Clearance:0.56||SGD DESC:Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis Gene:GPI15(YNL038W)|FD-Score:-3.34|P-value:4.20E-4|Clearance:0||SGD DESC:Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-H protein Gene:HSH49(YOR319W)|FD-Score:4.07|P-value:2.35E-5|Clearance:0.08||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:NAR1(YNL240C)|FD-Score:3.99|P-value:3.35E-5|Clearance:0.52||SGD DESC:Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf Gene:NSA2(YER126C)|FD-Score:3.47|P-value:2.61E-4|Clearance:0.49||SGD DESC:Protein constituent of 66S pre-ribosomal particles, contributes to processing of the 27S pre-rRNA Gene:RET3(YPL010W)|FD-Score:-3.15|P-value:8.17E-4|Clearance:0||SGD DESC:Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RSM10(YDR041W)|FD-Score:-3.64|P-value:1.37E-4|Clearance:0||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins Gene:RVB2(YPL235W)|FD-Score:5.1|P-value:1.73E-7|Clearance:0.56||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SGV1(YPR161C)|FD-Score:-3.31|P-value:4.70E-4|Clearance:0||SGD DESC:Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins Gene:SPP382(YLR424W)|FD-Score:5.43|P-value:2.76E-8|Clearance:0.56||SGD DESC:Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation Gene:ARP9(YMR033W)|FD-Score:4.13|P-value:1.79E-5|Clearance:0.06||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:ECM16(YMR128W)|FD-Score:4.7|P-value:1.32E-6|Clearance:0.56||SGD DESC:Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis Gene:GPI15(YNL038W)|FD-Score:-3.34|P-value:4.20E-4|Clearance:0||SGD DESC:Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-H protein Gene:HSH49(YOR319W)|FD-Score:4.07|P-value:2.35E-5|Clearance:0.08||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:NAR1(YNL240C)|FD-Score:3.99|P-value:3.35E-5|Clearance:0.52||SGD DESC:Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf Gene:NSA2(YER126C)|FD-Score:3.47|P-value:2.61E-4|Clearance:0.49||SGD DESC:Protein constituent of 66S pre-ribosomal particles, contributes to processing of the 27S pre-rRNA Gene:RET3(YPL010W)|FD-Score:-3.15|P-value:8.17E-4|Clearance:0||SGD DESC:Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RSM10(YDR041W)|FD-Score:-3.64|P-value:1.37E-4|Clearance:0||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins Gene:RVB2(YPL235W)|FD-Score:5.1|P-value:1.73E-7|Clearance:0.56||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SGV1(YPR161C)|FD-Score:-3.31|P-value:4.70E-4|Clearance:0||SGD DESC:Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins Gene:SPP382(YLR424W)|FD-Score:5.43|P-value:2.76E-8|Clearance:0.56||SGD DESC:Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4930451
Download HOP data (tab-delimited text)  (excel)
Gene:ATG34(YOL083W)|FD-Score:-4.36|P-value:6.38E-6||SGD DESC:Receptor protein involved in selective autophagy during starvation; specifically involved in the transport of cargo protein alpha-mannosidase (Ams1p); Atg19p paralog Gene:BTS1(YPL069C)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CSM1(YCR086W)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Nucleolar protein that forms a complex with Lrs4p and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:DAL7(YIR031C)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DFG16(YOR030W)|FD-Score:3.91|P-value:4.57E-5||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DSS1(YMR287C)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:EOS1(YNL080C)|FD-Score:3.96|P-value:3.81E-5||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:FMO1(YHR176W)|FD-Score:3.87|P-value:5.46E-5||SGD DESC:Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins Gene:FMP33(YJL161W_p)|FD-Score:-3.9|P-value:4.77E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FPS1(YLL043W)|FD-Score:3.33|P-value:4.30E-4||SGD DESC:Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress Gene:GCN4(YEL009C)|FD-Score:-3.73|P-value:9.65E-5||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GPD1(YDL022W)|FD-Score:-4.16|P-value:1.57E-5||SGD DESC:NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity Gene:IES4(YOR189W)|FD-Score:-3.44|P-value:2.96E-4||SGD DESC:Component of the INO80 chromatiin remodeling complex and target of the Mec1p/Tel1p DNA damage signaling pathway; proposed to link chromatin remodeling to replication checkpoint responses Gene:IMA5(YJL216C)|FD-Score:4.06|P-value:2.49E-5||SGD DESC:Alpha-glucosidase; specificity for isomaltose, maltose, and palatinose, but not alpha-methylglucoside; member of the IMA isomaltase family; not required for isomaltose utilization, but Ima5p overexpression allows the ima1 null mutant to grow on isomaltose Gene:IRC7(YFR055W_p)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Beta-lyase involved in the production of thiols; null mutant displays increased levels of spontaneous Rad52p foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner and by copper levels in a Mac1-dependent manner Gene:ISW1(YBR245C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p (Isw1a), and a complex with Ioc2p and Ioc4p (Isw1b); Isw1a and Isw1b have partially overlapping and distinct roles, Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation; Isw1b recruited to open reading frames by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions Gene:IZH2(YOL002C)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc Gene:KAR5(YMR065W)|FD-Score:-3.98|P-value:3.45E-5||SGD DESC:Protein required for nuclear membrane fusion during karyogamy, localizes to the membrane with a soluble portion in the endoplasmic reticulum lumen, may form a complex with Jem1p and Kar2p; expression of the gene is regulated by pheromone Gene:LEO1(YOR123C)|FD-Score:3.55|P-value:1.89E-4||SGD DESC:Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:LEU4(YNL104C)|FD-Score:-3.98|P-value:3.47E-5||SGD DESC:Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway; LEU4 has a paralog, LEU9, that arose from the whole genome duplication Gene:MCM16(YPR046W)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3 Gene:MSB3(YNL293W)|FD-Score:3.18|P-value:7.40E-4||SGD DESC:Rab GTPase-activating protein; regulates endocytosis via activation of Vps21p and Ypt7p; also acts on Ypt52p and Sec4p; also required for proper actin organization; localizes to the plasma membrane and sites of polarized growth, and a portion localizes to endosomes and vacuoles; similar to Msb4p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MSB4(YOL112W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization Gene:MSH2(YOL090W)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP Gene:MSH5(YDL154W)|FD-Score:-3.52|P-value:2.17E-4||SGD DESC:Protein of the MutS family, forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans Gene:MSS18(YPR134W)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions Gene:NAB6(YML117W)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP Gene:NPR2(YEL062W)|FD-Score:-3.38|P-value:3.64E-4||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; Iml1p interacts primarily with phosphorylated Npr2p; homolog of human NPRL2; target of Grr1p; required for growth on urea and proline Gene:NUM1(YDR150W)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:OPI6(YDL096C_d)|FD-Score:-3.25|P-value:5.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential Gene:ORT1(YOR130C)|FD-Score:-4.3|P-value:8.61E-6||SGD DESC:Ornithine transporter of the mitochondrial inner membrane, exports ornithine from mitochondria as part of arginine biosynthesis; human ortholog is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome Gene:PAM1(YDR251W)|FD-Score:-3.85|P-value:6.00E-5||SGD DESC:Essential protein of unknown function; exhibits variable expression during colony morphogenesis; overexpression permits survival without protein phosphatase 2A, inhibits growth, and induces a filamentous phenotype Gene:PAU24(YBR301W)|FD-Score:-3.6|P-value:1.58E-4||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:PFA3(YNL326C)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Palmitoyltransferase for Vac8p, required for vacuolar membrane fusion; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; autoacylates; required for vacuolar integrity under stress conditions Gene:POF1(YCL047C)|FD-Score:4.34|P-value:7.20E-6||SGD DESC:ATPase involved in the protein quality control and filamentation pathways; interacts physically with Kss1p and suppresses the filamentation defect of a kss1 deletion; also interacts with Ubc7p Gene:RIM21(YNL294C)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RPS14A(YCR031C)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RPS7A(YOR096W)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7A has a paralog, RPS7B, that arose from the whole genome duplication Gene:RTT101(YJL047C)|FD-Score:4.18|P-value:1.48E-5||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:SRO77(YBL106C)|FD-Score:3.36|P-value:3.86E-4||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:STD1(YOR047C)|FD-Score:-5.08|P-value:1.90E-7||SGD DESC:Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+] Gene:SWM2(YNR004W_p)|FD-Score:3.99|P-value:3.27E-5||SGD DESC:Putative protein of unknown function; haploid disruptant exhibits cold-sensitive growth and elongated buds Gene:TRE1(YPL176C)|FD-Score:3.26|P-value:5.67E-4||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:4.99|P-value:3.10E-7||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:UGX2(YDL169C)|FD-Score:3.14|P-value:8.47E-4||SGD DESC:Protein of unknown function, transcript accumulates in response to any combination of stress conditions Gene:UNG1(YML021C)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus Gene:URC2(YDR520C_p)|FD-Score:3.74|P-value:9.37E-5||SGD DESC:Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism Gene:VAC17(YCL063W)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p Gene:VPS20(YMR077C)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:YBR113W(YBR113W_d)|FD-Score:-3.18|P-value:7.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CYC8 Gene:YCL002C(YCL002C_p)|FD-Score:-4.05|P-value:2.54E-5||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YDR194W-A(YDR194W-A_p)|FD-Score:3.21|P-value:6.61E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR269C(YDR269C_d)|FD-Score:-3.12|P-value:8.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEH2(YLR020C)|FD-Score:-3.29|P-value:4.94E-4||SGD DESC:Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism Gene:YFL054C(YFL054C_p)|FD-Score:-5.03|P-value:2.51E-7||SGD DESC:Putative channel-like protein; similar to Fps1p; mediates passive diffusion of glycerol in the presence of ethanol Gene:YGK3(YOL128C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation Gene:YGL006W-A(YGL006W-A_p)|FD-Score:-3.19|P-value:7.11E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YGL199C(YGL199C_d)|FD-Score:-3.12|P-value:9.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YIP4/YGL198W Gene:YGR210C(YGR210C_p)|FD-Score:3.26|P-value:5.64E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YHR202W(YHR202W_p)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization Gene:YJR098C(YJR098C_p)|FD-Score:-3.54|P-value:2.03E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YKL131W(YKL131W_d)|FD-Score:-3.19|P-value:7.22E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR281C(YLR281C_p)|FD-Score:5.36|P-value:4.09E-8||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR281C is not an essential gene Gene:YMR242W-A(YMR242W-A_p)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Putative protein of unknown function Gene:YNL035C(YNL035C_p)|FD-Score:-3.23|P-value:6.29E-4||SGD DESC:Protein of unknown function; contains WD-40 domains; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YNL035C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YOR1(YGR281W)|FD-Score:4.74|P-value:1.05E-6||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR) Gene:YPL067C(YPL067C_p)|FD-Score:-3.52|P-value:2.12E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL067C is not an essential gene Gene:ATG34(YOL083W)|FD-Score:-4.36|P-value:6.38E-6||SGD DESC:Receptor protein involved in selective autophagy during starvation; specifically involved in the transport of cargo protein alpha-mannosidase (Ams1p); Atg19p paralog Gene:BTS1(YPL069C)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CSM1(YCR086W)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Nucleolar protein that forms a complex with Lrs4p and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:DAL7(YIR031C)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DFG16(YOR030W)|FD-Score:3.91|P-value:4.57E-5||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DSS1(YMR287C)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:EOS1(YNL080C)|FD-Score:3.96|P-value:3.81E-5||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:FMO1(YHR176W)|FD-Score:3.87|P-value:5.46E-5||SGD DESC:Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins Gene:FMP33(YJL161W_p)|FD-Score:-3.9|P-value:4.77E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FPS1(YLL043W)|FD-Score:3.33|P-value:4.30E-4||SGD DESC:Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress Gene:GCN4(YEL009C)|FD-Score:-3.73|P-value:9.65E-5||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GPD1(YDL022W)|FD-Score:-4.16|P-value:1.57E-5||SGD DESC:NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity Gene:IES4(YOR189W)|FD-Score:-3.44|P-value:2.96E-4||SGD DESC:Component of the INO80 chromatiin remodeling complex and target of the Mec1p/Tel1p DNA damage signaling pathway; proposed to link chromatin remodeling to replication checkpoint responses Gene:IMA5(YJL216C)|FD-Score:4.06|P-value:2.49E-5||SGD DESC:Alpha-glucosidase; specificity for isomaltose, maltose, and palatinose, but not alpha-methylglucoside; member of the IMA isomaltase family; not required for isomaltose utilization, but Ima5p overexpression allows the ima1 null mutant to grow on isomaltose Gene:IRC7(YFR055W_p)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Beta-lyase involved in the production of thiols; null mutant displays increased levels of spontaneous Rad52p foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner and by copper levels in a Mac1-dependent manner Gene:ISW1(YBR245C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p (Isw1a), and a complex with Ioc2p and Ioc4p (Isw1b); Isw1a and Isw1b have partially overlapping and distinct roles, Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation; Isw1b recruited to open reading frames by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions Gene:IZH2(YOL002C)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc Gene:KAR5(YMR065W)|FD-Score:-3.98|P-value:3.45E-5||SGD DESC:Protein required for nuclear membrane fusion during karyogamy, localizes to the membrane with a soluble portion in the endoplasmic reticulum lumen, may form a complex with Jem1p and Kar2p; expression of the gene is regulated by pheromone Gene:LEO1(YOR123C)|FD-Score:3.55|P-value:1.89E-4||SGD DESC:Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:LEU4(YNL104C)|FD-Score:-3.98|P-value:3.47E-5||SGD DESC:Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway; LEU4 has a paralog, LEU9, that arose from the whole genome duplication Gene:MCM16(YPR046W)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3 Gene:MSB3(YNL293W)|FD-Score:3.18|P-value:7.40E-4||SGD DESC:Rab GTPase-activating protein; regulates endocytosis via activation of Vps21p and Ypt7p; also acts on Ypt52p and Sec4p; also required for proper actin organization; localizes to the plasma membrane and sites of polarized growth, and a portion localizes to endosomes and vacuoles; similar to Msb4p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MSB4(YOL112W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization Gene:MSH2(YOL090W)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP Gene:MSH5(YDL154W)|FD-Score:-3.52|P-value:2.17E-4||SGD DESC:Protein of the MutS family, forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans Gene:MSS18(YPR134W)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions Gene:NAB6(YML117W)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP Gene:NPR2(YEL062W)|FD-Score:-3.38|P-value:3.64E-4||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; Iml1p interacts primarily with phosphorylated Npr2p; homolog of human NPRL2; target of Grr1p; required for growth on urea and proline Gene:NUM1(YDR150W)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:OPI6(YDL096C_d)|FD-Score:-3.25|P-value:5.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential Gene:ORT1(YOR130C)|FD-Score:-4.3|P-value:8.61E-6||SGD DESC:Ornithine transporter of the mitochondrial inner membrane, exports ornithine from mitochondria as part of arginine biosynthesis; human ortholog is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome Gene:PAM1(YDR251W)|FD-Score:-3.85|P-value:6.00E-5||SGD DESC:Essential protein of unknown function; exhibits variable expression during colony morphogenesis; overexpression permits survival without protein phosphatase 2A, inhibits growth, and induces a filamentous phenotype Gene:PAU24(YBR301W)|FD-Score:-3.6|P-value:1.58E-4||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:PFA3(YNL326C)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Palmitoyltransferase for Vac8p, required for vacuolar membrane fusion; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; autoacylates; required for vacuolar integrity under stress conditions Gene:POF1(YCL047C)|FD-Score:4.34|P-value:7.20E-6||SGD DESC:ATPase involved in the protein quality control and filamentation pathways; interacts physically with Kss1p and suppresses the filamentation defect of a kss1 deletion; also interacts with Ubc7p Gene:RIM21(YNL294C)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RPS14A(YCR031C)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RPS7A(YOR096W)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7A has a paralog, RPS7B, that arose from the whole genome duplication Gene:RTT101(YJL047C)|FD-Score:4.18|P-value:1.48E-5||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:SRO77(YBL106C)|FD-Score:3.36|P-value:3.86E-4||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:STD1(YOR047C)|FD-Score:-5.08|P-value:1.90E-7||SGD DESC:Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+] Gene:SWM2(YNR004W_p)|FD-Score:3.99|P-value:3.27E-5||SGD DESC:Putative protein of unknown function; haploid disruptant exhibits cold-sensitive growth and elongated buds Gene:TRE1(YPL176C)|FD-Score:3.26|P-value:5.67E-4||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:4.99|P-value:3.10E-7||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:UGX2(YDL169C)|FD-Score:3.14|P-value:8.47E-4||SGD DESC:Protein of unknown function, transcript accumulates in response to any combination of stress conditions Gene:UNG1(YML021C)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus Gene:URC2(YDR520C_p)|FD-Score:3.74|P-value:9.37E-5||SGD DESC:Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism Gene:VAC17(YCL063W)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p Gene:VPS20(YMR077C)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:YBR113W(YBR113W_d)|FD-Score:-3.18|P-value:7.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CYC8 Gene:YCL002C(YCL002C_p)|FD-Score:-4.05|P-value:2.54E-5||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YDR194W-A(YDR194W-A_p)|FD-Score:3.21|P-value:6.61E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR269C(YDR269C_d)|FD-Score:-3.12|P-value:8.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEH2(YLR020C)|FD-Score:-3.29|P-value:4.94E-4||SGD DESC:Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism Gene:YFL054C(YFL054C_p)|FD-Score:-5.03|P-value:2.51E-7||SGD DESC:Putative channel-like protein; similar to Fps1p; mediates passive diffusion of glycerol in the presence of ethanol Gene:YGK3(YOL128C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation Gene:YGL006W-A(YGL006W-A_p)|FD-Score:-3.19|P-value:7.11E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YGL199C(YGL199C_d)|FD-Score:-3.12|P-value:9.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YIP4/YGL198W Gene:YGR210C(YGR210C_p)|FD-Score:3.26|P-value:5.64E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YHR202W(YHR202W_p)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization Gene:YJR098C(YJR098C_p)|FD-Score:-3.54|P-value:2.03E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YKL131W(YKL131W_d)|FD-Score:-3.19|P-value:7.22E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR281C(YLR281C_p)|FD-Score:5.36|P-value:4.09E-8||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR281C is not an essential gene Gene:YMR242W-A(YMR242W-A_p)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Putative protein of unknown function Gene:YNL035C(YNL035C_p)|FD-Score:-3.23|P-value:6.29E-4||SGD DESC:Protein of unknown function; contains WD-40 domains; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YNL035C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YOR1(YGR281W)|FD-Score:4.74|P-value:1.05E-6||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR) Gene:YPL067C(YPL067C_p)|FD-Score:-3.52|P-value:2.12E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL067C is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR424W5.432.76E-80.56SPP382Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation
YPL235W5.101.73E-70.56RVB2ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly
YMR128W4.701.32E-60.56ECM16Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis
YMR033W4.131.79E-50.06ARP9Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YOR319W4.072.35E-50.08HSH49U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
YNL240C3.993.35E-50.52NAR1Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf
YER126C3.472.61E-40.49NSA2Protein constituent of 66S pre-ribosomal particles, contributes to processing of the 27S pre-rRNA
YHR007C2.980.001450.07ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YHR036W2.910.001790.09BRL1Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export
YKL028W2.820.002380.12TFA1TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening
YOL123W2.710.003400.01HRP1Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences
YDR280W2.690.003530.00RRP45Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
YLR208W2.690.003560.03SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YIL083C2.660.003910.01CAB2Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity)
YDR489W2.650.004080.04SLD5Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR281C_p5.364.09E-8YLR281C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR281C is not an essential gene
YER090W4.993.10E-7TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YGR281W4.741.05E-6YOR1Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR)
YCL047C4.347.20E-6POF1ATPase involved in the protein quality control and filamentation pathways; interacts physically with Kss1p and suppresses the filamentation defect of a kss1 deletion; also interacts with Ubc7p
YJL047C4.181.48E-5RTT101Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p
YJL216C4.062.49E-5IMA5Alpha-glucosidase; specificity for isomaltose, maltose, and palatinose, but not alpha-methylglucoside; member of the IMA isomaltase family; not required for isomaltose utilization, but Ima5p overexpression allows the ima1 null mutant to grow on isomaltose
YNR004W_p3.993.27E-5SWM2_pPutative protein of unknown function; haploid disruptant exhibits cold-sensitive growth and elongated buds
YNL080C3.963.81E-5EOS1Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene
YOR030W3.914.57E-5DFG16Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p
YHR176W3.875.46E-5FMO1Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins
YDR520C_p3.749.37E-5URC2_pPutative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism
YOL128C3.721.01E-4YGK3Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation
YBR245C3.701.07E-4ISW1ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p (Isw1a), and a complex with Ioc2p and Ioc4p (Isw1b); Isw1a and Isw1b have partially overlapping and distinct roles, Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation; Isw1b recruited to open reading frames by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions
YMR242W-A_p3.651.32E-4YMR242W-A_pPutative protein of unknown function
YOL112W3.641.36E-4MSB4GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization

GO enrichment analysis for SGTC_2864
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0758.75E-9SGTC_13903413-2614 145.0 μMChemDiv (Drug-like library)34214320.0142857fatty acid desaturase (OLE1)
0.0759.38E-9SGTC_14554262-0120 131.0 μMChemDiv (Drug-like library)15295870.0983607plasma membrane duress
0.0741.05E-8SGTC_23619034121 116.0 μMChembridge (Fragment library)64969170.102941RSC complex & mRNA processing
0.0715.38E-8SGTC_28187996756 13.0 μMChembridge (Drug-like library)22416210.168831
0.0706.70E-8SGTC_255413-methyl-4,4-bisnor-8,11,13-podocarpatrien-3-one 66.7 μMMicrosource (Natural product library)67085610.0675676
0.0707.10E-8SGTC_2648atorvastatin 78.7 μMMiscellaneous608230.183908azole & statin
0.0681.56E-7SGTC_2560caryophyllene 100.0 μMMicrosource (Natural product library)166676880.0428571
0.0672.61E-7SGTC_1136pergolide 1.1 μMTimTec (Natural product library)478110.0813954
0.0664.52E-7SGTC_2665lovastatin 100.0 μMMiscellaneous532320.0957447
0.0655.02E-7SGTC_1912st060209 90.8 μMTimTec (Natural product derivative library)6887870.2
0.0655.62E-7SGTC_13913442-0225 151.0 μMChemDiv (Drug-like library)52270750.107692
0.0621.98E-6SGTC_1952st077036 64.1 μMTimTec (Natural product derivative library)29370070.144737
0.0603.36E-6SGTC_23047443016 91.4 μMChembridge (Fragment library)9398540.0675676
0.0604.19E-6SGTC_1850st056238 17.0 μMTimTec (Natural product derivative library)53931770.0821918RPP1 & pyrimidine depletion
0.0595.81E-6SGTC_22037227154 166.4 μMChembridge (Fragment library)8963040.2

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_9861493-048384 μM0.57142940079ChemDiv (Drug-like library)256.256681.31914
SGTC_9383126-1755342 μM0.4905661777771ChemDiv (Drug-like library)390.388723.01235
SGTC_11770983-020423.6 μM0.481481766818ChemDiv (Drug-like library)318.369123.99613
SGTC_2879904181571.43 μM0.4814814942971Chembridge (Drug-like library)289.756744.13512
SGTC_3184910875849.47 μM0.480769902885Chembridge (Drug-like library)241.28512.94712
SGTC_2998906676271.43 μM0.4736844945196Chembridge (Drug-like library)297.348363.54713RPP1 & pyrimidine depletion
SGTC_3181910827749.47 μM0.44067817097298Chembridge (Drug-like library)284.35293.02813iron homeostasis
SGTC_21245246320200 μM0.44579342Chembridge (Fragment library)216.2110631.66913heme biosynthesis & mitochondrial translocase
SGTC_3250913558349.47 μM0.4363647699353Chembridge (Drug-like library)299.321182.80214
SGTC_10553448-067286 μM0.425926748378ChemDiv (Drug-like library)302.369723.92412