9034245

2,4-dichloro-N-(2-methylquinolin-5-yl)benzamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2866
Screen concentration 58.4 μM
Source Chembridge (Drug-like library)
PubChem CID 6462273
SMILES CC1=NC2=C(C=C1)C(=CC=C2)NC(=O)C3=C(C=C(C=C3)Cl)Cl
Standardized SMILES Cc1ccc2c(NC(=O)c3ccc(Cl)cc3Cl)cccc2n1
Molecular weight 331.196
ALogP 4.41
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 5.75
% growth inhibition (Hom. pool) 4.18


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6462273
Download HIP data (tab-delimited text)  (excel)
Gene:ACC1(YNR016C)|FD-Score:3.59|P-value:1.67E-4|Clearance:0.45||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ARP4(YJL081C)|FD-Score:3.14|P-value:8.46E-4|Clearance:0.22||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ATM1(YMR301C)|FD-Score:-3.29|P-value:5.05E-4|Clearance:0||SGD DESC:Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol Gene:LSM3(YLR438C-A)|FD-Score:-3.11|P-value:9.24E-4|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:PKC1(YBL105C)|FD-Score:4.11|P-value:1.94E-5|Clearance:0.53||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:TEM1(YML064C)|FD-Score:-3.19|P-value:7.16E-4|Clearance:0||SGD DESC:GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis Gene:ACC1(YNR016C)|FD-Score:3.59|P-value:1.67E-4|Clearance:0.45||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ARP4(YJL081C)|FD-Score:3.14|P-value:8.46E-4|Clearance:0.22||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ATM1(YMR301C)|FD-Score:-3.29|P-value:5.05E-4|Clearance:0||SGD DESC:Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol Gene:LSM3(YLR438C-A)|FD-Score:-3.11|P-value:9.24E-4|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:PKC1(YBL105C)|FD-Score:4.11|P-value:1.94E-5|Clearance:0.53||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:TEM1(YML064C)|FD-Score:-3.19|P-value:7.16E-4|Clearance:0||SGD DESC:GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6462273
Download HOP data (tab-delimited text)  (excel)
Gene:AEP2(YMR282C)|FD-Score:4.51|P-value:3.28E-6||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:ATG16(YMR159C)|FD-Score:3.2|P-value:6.99E-4||SGD DESC:Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which localize to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:ATP4(YPL078C)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Subunit b of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; contributes to the oligomerization of the complex; phosphorylated Gene:BPH1(YCR032W)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting Gene:BUD19(YJL188C_d)|FD-Score:4.45|P-value:4.22E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:DAL4(YIR028W)|FD-Score:5.36|P-value:4.22E-8||SGD DESC:Allantoin permease; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DAL7(YIR031C)|FD-Score:3.1|P-value:9.68E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DAP2(YHR028C)|FD-Score:-7.42|P-value:5.68E-14||SGD DESC:Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p Gene:IES2(YNL215W)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress Gene:IRA2(YOL081W)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication Gene:ISM1(YPL040C)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:LOA1(YPR139C)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA Gene:LSM6(YDR378C)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MRPS17(YMR188C)|FD-Score:3.72|P-value:9.92E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NRG2(YBR066C)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:Transcriptional repressor; mediates glucose repression and negatively regulates filamentous growth; NRG2 has a paralog, NRG1, that arose from the whole genome duplication Gene:NTA1(YJR062C)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:Amidase, removes the amide group from N-terminal asparagine and glutamine residues to generate proteins with N-terminal aspartate and glutamate residues that are targets of ubiquitin-mediated degradation Gene:POC4(YPL144W)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:PPA2(YMR267W)|FD-Score:3.24|P-value:5.91E-4||SGD DESC:Mitochondrial inorganic pyrophosphatase, required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate Gene:PPN1(YDR452W)|FD-Score:-3.82|P-value:6.61E-5||SGD DESC:Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:PTC4(YBR125C)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity Gene:RPS14A(YCR031C)|FD-Score:3.1|P-value:9.74E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RTC6(YPL183W-A)|FD-Score:3.14|P-value:8.34E-4||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:SSP2(YOR242C)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Sporulation specific protein that localizes to the spore wall; required for sporulation at a point after meiosis II and during spore wall formation; SSP2 expression is induced midway in meiosis Gene:TCM62(YBR044C)|FD-Score:3.82|P-value:6.57E-5||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; putative chaperone Gene:TDA9(YML081W)|FD-Score:-4.21|P-value:1.28E-5||SGD DESC:DNA-binding protein, putative transcription factor; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; TDA9 has a paralog, RSF2, that arose from the whole genome duplication Gene:TGL4(YKR089C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Multifunctional triacylglycerol lipase, steryl ester hydrolase, and Ca2+-independent phospholipase A2; catalyzes acyl-CoA dependent acylation of LPA to PA; required with Tgl3p for timely bud formation; phosphorylated and activated by Cdc28p Gene:TKL1(YPR074C)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:-4.98|P-value:3.21E-7||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:-3.4|P-value:3.37E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UBX2(YML013W)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:YAL066W(YAL066W_d)|FD-Score:-3.78|P-value:7.81E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YAL067W-A(YAL067W-A_p)|FD-Score:3.15|P-value:8.08E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YDR344C(YDR344C_d)|FD-Score:3.32|P-value:4.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEL1(YBL060W)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip Gene:YET2(YMR040W)|FD-Score:-3.11|P-value:9.41E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein Gene:YFR006W(YFR006W_p)|FD-Score:4.19|P-value:1.42E-5||SGD DESC:Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene Gene:YIL067C(YIL067C_p)|FD-Score:4.35|P-value:6.95E-6||SGD DESC:Uncharacterized protein of unknown function Gene:YJL160C(YJL160C_p)|FD-Score:-3.72|P-value:9.82E-5||SGD DESC:Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication Gene:YLR455W(YLR455W_p)|FD-Score:3.85|P-value:5.94E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress Gene:YMR253C(YMR253C_p)|FD-Score:3.91|P-value:4.59E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene Gene:YNL140C(YNL140C_p)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C Gene:YPL136W(YPL136W_d)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene GIP3/YPL137C Gene:ZEO1(YOL109W)|FD-Score:-3.22|P-value:6.50E-4||SGD DESC:Peripheral membrane protein of the plasma membrane that interacts with Mid2p; regulates the cell integrity pathway mediated by Pkc1p and Slt2p; the authentic protein is detected in a phosphorylated state in highly purified mitochondria Gene:AEP2(YMR282C)|FD-Score:4.51|P-value:3.28E-6||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:ATG16(YMR159C)|FD-Score:3.2|P-value:6.99E-4||SGD DESC:Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which localize to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:ATP4(YPL078C)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Subunit b of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; contributes to the oligomerization of the complex; phosphorylated Gene:BPH1(YCR032W)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting Gene:BUD19(YJL188C_d)|FD-Score:4.45|P-value:4.22E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:DAL4(YIR028W)|FD-Score:5.36|P-value:4.22E-8||SGD DESC:Allantoin permease; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DAL7(YIR031C)|FD-Score:3.1|P-value:9.68E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DAP2(YHR028C)|FD-Score:-7.42|P-value:5.68E-14||SGD DESC:Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p Gene:IES2(YNL215W)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress Gene:IRA2(YOL081W)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication Gene:ISM1(YPL040C)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:LOA1(YPR139C)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA Gene:LSM6(YDR378C)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MRPS17(YMR188C)|FD-Score:3.72|P-value:9.92E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NRG2(YBR066C)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:Transcriptional repressor; mediates glucose repression and negatively regulates filamentous growth; NRG2 has a paralog, NRG1, that arose from the whole genome duplication Gene:NTA1(YJR062C)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:Amidase, removes the amide group from N-terminal asparagine and glutamine residues to generate proteins with N-terminal aspartate and glutamate residues that are targets of ubiquitin-mediated degradation Gene:POC4(YPL144W)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:PPA2(YMR267W)|FD-Score:3.24|P-value:5.91E-4||SGD DESC:Mitochondrial inorganic pyrophosphatase, required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate Gene:PPN1(YDR452W)|FD-Score:-3.82|P-value:6.61E-5||SGD DESC:Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:PTC4(YBR125C)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity Gene:RPS14A(YCR031C)|FD-Score:3.1|P-value:9.74E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RTC6(YPL183W-A)|FD-Score:3.14|P-value:8.34E-4||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:SSP2(YOR242C)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Sporulation specific protein that localizes to the spore wall; required for sporulation at a point after meiosis II and during spore wall formation; SSP2 expression is induced midway in meiosis Gene:TCM62(YBR044C)|FD-Score:3.82|P-value:6.57E-5||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; putative chaperone Gene:TDA9(YML081W)|FD-Score:-4.21|P-value:1.28E-5||SGD DESC:DNA-binding protein, putative transcription factor; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; TDA9 has a paralog, RSF2, that arose from the whole genome duplication Gene:TGL4(YKR089C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Multifunctional triacylglycerol lipase, steryl ester hydrolase, and Ca2+-independent phospholipase A2; catalyzes acyl-CoA dependent acylation of LPA to PA; required with Tgl3p for timely bud formation; phosphorylated and activated by Cdc28p Gene:TKL1(YPR074C)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:-4.98|P-value:3.21E-7||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:-3.4|P-value:3.37E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UBX2(YML013W)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:YAL066W(YAL066W_d)|FD-Score:-3.78|P-value:7.81E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YAL067W-A(YAL067W-A_p)|FD-Score:3.15|P-value:8.08E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YDR344C(YDR344C_d)|FD-Score:3.32|P-value:4.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEL1(YBL060W)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip Gene:YET2(YMR040W)|FD-Score:-3.11|P-value:9.41E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein Gene:YFR006W(YFR006W_p)|FD-Score:4.19|P-value:1.42E-5||SGD DESC:Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene Gene:YIL067C(YIL067C_p)|FD-Score:4.35|P-value:6.95E-6||SGD DESC:Uncharacterized protein of unknown function Gene:YJL160C(YJL160C_p)|FD-Score:-3.72|P-value:9.82E-5||SGD DESC:Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication Gene:YLR455W(YLR455W_p)|FD-Score:3.85|P-value:5.94E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress Gene:YMR253C(YMR253C_p)|FD-Score:3.91|P-value:4.59E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene Gene:YNL140C(YNL140C_p)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C Gene:YPL136W(YPL136W_d)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene GIP3/YPL137C Gene:ZEO1(YOL109W)|FD-Score:-3.22|P-value:6.50E-4||SGD DESC:Peripheral membrane protein of the plasma membrane that interacts with Mid2p; regulates the cell integrity pathway mediated by Pkc1p and Slt2p; the authentic protein is detected in a phosphorylated state in highly purified mitochondria

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBL105C4.111.94E-50.53PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YNR016C3.591.67E-40.45ACC1Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
YJL081C3.148.46E-40.22ARP4Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
YCR052W2.920.001740.04RSC6Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p
YGL093W2.880.001990.17SPC105Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components
YER172C2.710.003330.01BRR2RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD
YMR200W2.700.003460.14ROT1Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation
YBR091C2.560.005200.12TIM12Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins
YDR288W2.440.007370.01NSE3Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress
YIL075C2.430.007620.01RPN2Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress
YOL069W2.420.007840.19NUF2Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering
YDR437W2.230.013000.01GPI19Subunit of GPI-GlcNAc transferase involved in synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), which is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis, shares similarity with mammalian PIG-P
YLL018C2.210.013400.00DPS1Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation
YGL130W2.210.013600.00CEG1Alpha (guanylyltransferase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CET1, an RNA 5'-triphosphatase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide
YDL097C2.210.013700.09RPN6Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YIR028W5.364.22E-8DAL4Allantoin permease; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation
YMR282C4.513.28E-6AEP2Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader
YJL188C_d4.454.22E-6BUD19_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay
YIL067C_p4.356.95E-6YIL067C_pUncharacterized protein of unknown function
YBL060W4.211.26E-5YEL1Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip
YFR006W_p4.191.42E-5YFR006W_pPutative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene
YML013W4.181.45E-5UBX2Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis
YMR253C_p3.914.59E-5YMR253C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene
YLR455W_p3.855.94E-5YLR455W_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress
YBR044C3.826.57E-5TCM62Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; putative chaperone
YMR188C3.729.92E-5MRPS17Mitochondrial ribosomal protein of the small subunit
YKR089C3.591.63E-4TGL4Multifunctional triacylglycerol lipase, steryl ester hydrolase, and Ca2+-independent phospholipase A2; catalyzes acyl-CoA dependent acylation of LPA to PA; required with Tgl3p for timely bud formation; phosphorylated and activated by Cdc28p
YNL140C_p3.581.74E-4YNL140C_pProtein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C
YPL040C3.522.18E-4ISM1Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth
YCR032W3.522.18E-4BPH1Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting

GO enrichment analysis for SGTC_2866
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1983.13E-53SGTC_28619024595 19.5 μMChembridge (Drug-like library)164828100.24
0.1524.78E-32SGTC_28799041815 71.4 μMChembridge (Drug-like library)49429710.274194
0.0772.58E-9SGTC_28257998094 58.4 μMChembridge (Drug-like library)29878150.0813954fatty acid desaturase (OLE1)
0.0772.69E-9SGTC_28639028737 58.4 μMChembridge (Drug-like library)64609170.611111
0.0772.76E-9SGTC_32409133527 49.5 μMChembridge (Drug-like library)176174550.0886076
0.0741.03E-8SGTC_2547epiafzelechin trimethyl ether 100.0 μMMicrosource (Natural product library)67086300.0666667
0.0741.43E-8SGTC_23759072683 200.0 μMChembridge (Fragment library)167980290.0972222mitochondrial processes
0.0732.05E-8SGTC_1652st011932 78.0 μMTimTec (Natural product derivative library)15510760.106667RSC complex & mRNA processing
0.0715.09E-8SGTC_20785212016 200.0 μMChembridge (Fragment library)18997080.11111160S ribosome export
0.0708.87E-8SGTC_6140462-0418 107.0 μMChemDiv (Drug-like library)51883030.0895522
0.0708.77E-8SGTC_32309131665 49.5 μMChembridge (Drug-like library)159437200.102941
0.0691.28E-7SGTC_2747doxorubicin 7.6 μMMiscellaneous317030.0857143anthracycline transcription coupled DNA repair
0.0656.58E-7SGTC_3101273-0058 1.1 μMChemDiv (Drug-like library)27342320.0307692mitochondrial stress
0.0647.60E-7SGTC_11333473-0247 50.0 μMChemDiv (Drug-like library)7113110.116883RSC complex & mRNA processing
0.0648.44E-7SGTC_20415107442 45.3 μMChembridge (Fragment library)7930050.0625RSC complex & mRNA processing

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_2863902873758.44 μM0.6111116460917Chembridge (Drug-like library)322.357823.0514
SGTC_12510490-5699265 μM0.4259263883818ChemDiv (Drug-like library)301.555783.66612
SGTC_2942905277762.25 μM0.4074076469503Chembridge (Drug-like library)284.1131234.14912
SGTC_7600568-072582.9 μM0.3928573916171ChemDiv (Drug-like library)380.010345.16613
SGTC_21065357045167.29 μM0.385965668842Chembridge (Fragment library)281.13733.01412
SGTC_2872904403725.97 μM0.3773586465195Chembridge (Drug-like library)284.1131234.14912
SGTC_7051124-0212310 μM0.3759554485ChemDiv (Drug-like library)293.1484.01212
SGTC_9791426-2828121 μM0.368421747872ChemDiv (Drug-like library)270.114662.88412
SGTC_6074491-098666.8 μM0.362069698981ChemDiv (Drug-like library)296.793985.48512heme requiring
SGTC_8261683-5028118 μM0.350877722377ChemDiv (Drug-like library)261.703583.26212