9035608

[3-[(3,4-dichlorophenyl)methoxy]phenyl]-morpholin-4-ylmethanone

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2868
Screen concentration 32.5 μM
Source Chembridge (Drug-like library)
PubChem CID 6462614
SMILES C1COCCN1C(=O)C2=CC(=CC=C2)OCC3=CC(=C(C=C3)Cl)Cl
Standardized SMILES Clc1ccc(COc2cccc(c2)C(=O)N3CCOCC3)cc1Cl
Molecular weight 366.2385
ALogP 3.83
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.63
% growth inhibition (Hom. pool) 11.78


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6462614
Download HIP data (tab-delimited text)  (excel)
Gene:ERG10(YPL028W)|FD-Score:4.58|P-value:2.36E-6|Clearance:0.5||SGD DESC:Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis Gene:MPE1(YKL059C)|FD-Score:-3.69|P-value:1.11E-4|Clearance:0||SGD DESC:Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif Gene:NAB3(YPL190C)|FD-Score:-3.17|P-value:7.71E-4|Clearance:0||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing Gene:NOP8(YOL144W)|FD-Score:4.32|P-value:7.82E-6|Clearance:0.5||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:NUP145(YGL092W)|FD-Score:3.82|P-value:6.67E-5|Clearance:0.27||SGD DESC:Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98 Gene:PIK1(YNL267W)|FD-Score:3.34|P-value:4.22E-4|Clearance:0.11||SGD DESC:Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton Gene:RPL30(YGL030W)|FD-Score:3.55|P-value:1.94E-4|Clearance:0.21||SGD DESC:Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog Gene:YCR013C(YCR013C_d)|FD-Score:-4.26|P-value:1.02E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YCR013C and the overlapping essential gene PGK1 is reduced in a gcr1 null mutant Gene:YCS4(YLR272C)|FD-Score:-3.21|P-value:6.62E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YPR177C(YPR177C_d)|FD-Score:3.23|P-value:6.14E-4|Clearance:0.21||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex Gene:ERG10(YPL028W)|FD-Score:4.58|P-value:2.36E-6|Clearance:0.5||SGD DESC:Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis Gene:MPE1(YKL059C)|FD-Score:-3.69|P-value:1.11E-4|Clearance:0||SGD DESC:Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif Gene:NAB3(YPL190C)|FD-Score:-3.17|P-value:7.71E-4|Clearance:0||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing Gene:NOP8(YOL144W)|FD-Score:4.32|P-value:7.82E-6|Clearance:0.5||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:NUP145(YGL092W)|FD-Score:3.82|P-value:6.67E-5|Clearance:0.27||SGD DESC:Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98 Gene:PIK1(YNL267W)|FD-Score:3.34|P-value:4.22E-4|Clearance:0.11||SGD DESC:Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton Gene:RPL30(YGL030W)|FD-Score:3.55|P-value:1.94E-4|Clearance:0.21||SGD DESC:Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog Gene:YCR013C(YCR013C_d)|FD-Score:-4.26|P-value:1.02E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YCR013C and the overlapping essential gene PGK1 is reduced in a gcr1 null mutant Gene:YCS4(YLR272C)|FD-Score:-3.21|P-value:6.62E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YPR177C(YPR177C_d)|FD-Score:3.23|P-value:6.14E-4|Clearance:0.21||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6462614
Download HOP data (tab-delimited text)  (excel)
Gene:AIM26(YKL037W)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:ASG7(YJL170C)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Protein that regulates signaling from a G protein beta subunit Ste4p and its relocalization within the cell; specific to a-cells and induced by alpha-factor Gene:ATF1(YOR377W)|FD-Score:4.59|P-value:2.19E-6||SGD DESC:Alcohol acetyltransferase; responsible for the major part of volatile acetate ester production during fermentation; main enzyme involved in terpenyl acetate synthesis; potential roles in lipid and sterol metabolism Gene:ATG34(YOL083W)|FD-Score:-4.55|P-value:2.69E-6||SGD DESC:Receptor protein involved in selective autophagy during starvation; specifically involved in the transport of cargo protein alpha-mannosidase (Ams1p); Atg19p paralog Gene:ATG5(YPL149W)|FD-Score:-3.55|P-value:1.96E-4||SGD DESC:Conserved protein involved in autophagy and the Cvt pathway; undergoes conjugation with Atg12p to form a complex involved in Atg8p lipidation; conjugated Atg12p also forms a complex with Atg16p that is essential for autophagosome formation Gene:BRE1(YDL074C)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:BRP1(YGL007W_d)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:CCE1(YKL011C)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA Gene:CHO2(YGR157W)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Phosphatidylethanolamine methyltransferase (PEMT), catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis Gene:CLA4(YNL298W)|FD-Score:-3.94|P-value:4.07E-5||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CLN2(YPL256C)|FD-Score:-3.54|P-value:1.98E-4||SGD DESC:G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) Gene:DAP2(YHR028C)|FD-Score:5.03|P-value:2.47E-7||SGD DESC:Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p Gene:DIA3(YDL024C)|FD-Score:7.15|P-value:4.30E-13||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth Gene:EMC3(YKL207W)|FD-Score:-3.25|P-value:5.81E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; required for respiratory growth; null mutant displays induction of the unfolded protein response; homologous to worm Y62E10A.10/EMC-3, fly CG6750, human TMEM111 Gene:EMP24(YGL200C)|FD-Score:-3.14|P-value:8.35E-4||SGD DESC:Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles Gene:EOS1(YNL080C)|FD-Score:4.1|P-value:2.05E-5||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:ETR1(YBR026C)|FD-Score:3.79|P-value:7.38E-5||SGD DESC:2-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis Gene:FMP27(YLR454W_p)|FD-Score:3.92|P-value:4.50E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRE6(YLL051C)|FD-Score:-3.51|P-value:2.20E-4||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels Gene:FRT1(YOR324C)|FD-Score:-3.13|P-value:8.71E-4||SGD DESC:Tail-anchored ER membrane protein of unknown function; substrate of the phosphatase calcineurin; interacts with homolog Frt2p; promotes cell growth in stress conditions, possibly via a role in posttranslational translocation; FRT1 has a paralog, FRT2, that arose from the whole genome duplication Gene:GCY1(YOR120W)|FD-Score:3.13|P-value:8.61E-4||SGD DESC:Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication Gene:GEP4(YHR100C)|FD-Score:-3.39|P-value:3.45E-4||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GRX1(YCL035C)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Glutathione-dependent disulfide oxidoreductase; hydroperoxide and superoxide-radical responsive, heat-stable, with active site cysteine pair; protects cells from oxidative damage; GRX1 has a paralog, GRX2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:HHO1(YPL127C)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Histone H1, linker histone with roles in meiosis and sporulation; decreasing levels early in sporulation may promote meiosis, and increasing levels during sporulation facilitate compaction of spore chromatin; binds to promoters and within genes in mature spores; may be recruited by Ume6p to promoter regions, contributing to transcriptional repression outside of meiosis; suppresses DNA repair involving homologous recombination Gene:INO1(YJL153C)|FD-Score:5.82|P-value:2.94E-9||SGD DESC:Inositol-3-phosphate synthase, involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element Gene:LOC1(YFR001W)|FD-Score:3.39|P-value:3.56E-4||SGD DESC:Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:LYS14(YDR034C)|FD-Score:-3.21|P-value:6.55E-4||SGD DESC:Transcriptional activator involved in regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer Gene:MID2(YLR332W)|FD-Score:3.15|P-value:8.09E-4||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:MRPL23(YOR150W)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NCS2(YNL119W)|FD-Score:-3.32|P-value:4.49E-4||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:OGG1(YML060W)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance Gene:PFK27(YOL136C)|FD-Score:4.27|P-value:9.62E-6||SGD DESC:6-phosphofructo-2-kinase, catalyzes synthesis of fructose-2,6-bisphosphate; inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate, expression induced by glucose and sucrose, transcriptional regulation involves protein kinase A Gene:PTH2(YBL057C)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:QRI5(YLR204W)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:REX2(YLR059C)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease Gene:RGS2(YOR107W)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p Gene:RIM101(YHL027W)|FD-Score:3.11|P-value:9.22E-4||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:ROM2(YLR371W)|FD-Score:4.62|P-value:1.92E-6||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:SAC1(YKL212W)|FD-Score:6.4|P-value:7.67E-11||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SHU1(YHL006C)|FD-Score:-4.42|P-value:4.83E-6||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SHU2(YDR078C)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SNF2(YOR290C)|FD-Score:-3.38|P-value:3.59E-4||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SPT23(YKL020C)|FD-Score:-3.26|P-value:5.62E-4||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:STI1(YOR027W)|FD-Score:3.76|P-value:8.53E-5||SGD DESC:Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop Gene:STP2(YHR006W)|FD-Score:-4.23|P-value:1.15E-5||SGD DESC:Transcription factor; activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes; STP2 has a paralog, STP1, that arose from the whole genome duplication Gene:SVL3(YPL032C)|FD-Score:4.27|P-value:9.66E-6||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:TVP38(YKR088C)|FD-Score:5.2|P-value:9.73E-8||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:VID22(YLR373C)|FD-Score:-3.1|P-value:9.56E-4||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:YAP1802(YGR241C)|FD-Score:4.37|P-value:6.24E-6||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication Gene:YDC1(YPL087W)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Alkaline dihydroceramidase, involved in sphingolipid metabolism; preferentially hydrolyzes dihydroceramide to a free fatty acid and dihydrosphingosine; has a minor reverse activity; YDC1 has a paralog, YPC1, that arose from the whole genome duplication Gene:YDL026W(YDL026W_d)|FD-Score:-3.31|P-value:4.68E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER130C(YER130C_p)|FD-Score:4.3|P-value:8.58E-6||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YFL019C(YFL019C_d)|FD-Score:3.18|P-value:7.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Gene:YGR064W(YGR064W_d)|FD-Score:-3.29|P-value:5.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YIR020W-A(YIR020W-A_d)|FD-Score:4.38|P-value:6.05E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YLR374C(YLR374C_d)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W Gene:YMR007W(YMR007W_d)|FD-Score:-3.54|P-value:1.98E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR057C(YMR057C_d)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF AAC1 Gene:YMR130W(YMR130W_p)|FD-Score:-3.12|P-value:9.17E-4||SGD DESC:Putative protein of unknown function; YMR130W is not an essential gene Gene:YNL319W(YNL319W_d)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HXT14 Gene:YNR066C(YNR066C_p)|FD-Score:3.72|P-value:9.88E-5||SGD DESC:Putative membrane-localized protein of unknown function Gene:YOL013W-A(YOL013W-A_p)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YOL019W(YOL019W_p)|FD-Score:4.46|P-value:4.04E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; YOL019W has a paralog, DCV1, that arose from the whole genome duplication Gene:YPL041C(YPL041C_p)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Protein of unknown function involved in maintenance of proper telomere length Gene:YPL260W(YPL260W_p)|FD-Score:3.25|P-value:5.67E-4||SGD DESC:Putative substrate of cAMP-dependent protein kinase (PKA); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YPL260W is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YPR039W(YPR039W_d)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W Gene:YPR099C(YPR099C_d)|FD-Score:3.27|P-value:5.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL51/YPR100W Gene:AIM26(YKL037W)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:ASG7(YJL170C)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Protein that regulates signaling from a G protein beta subunit Ste4p and its relocalization within the cell; specific to a-cells and induced by alpha-factor Gene:ATF1(YOR377W)|FD-Score:4.59|P-value:2.19E-6||SGD DESC:Alcohol acetyltransferase; responsible for the major part of volatile acetate ester production during fermentation; main enzyme involved in terpenyl acetate synthesis; potential roles in lipid and sterol metabolism Gene:ATG34(YOL083W)|FD-Score:-4.55|P-value:2.69E-6||SGD DESC:Receptor protein involved in selective autophagy during starvation; specifically involved in the transport of cargo protein alpha-mannosidase (Ams1p); Atg19p paralog Gene:ATG5(YPL149W)|FD-Score:-3.55|P-value:1.96E-4||SGD DESC:Conserved protein involved in autophagy and the Cvt pathway; undergoes conjugation with Atg12p to form a complex involved in Atg8p lipidation; conjugated Atg12p also forms a complex with Atg16p that is essential for autophagosome formation Gene:BRE1(YDL074C)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:BRP1(YGL007W_d)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:CCE1(YKL011C)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA Gene:CHO2(YGR157W)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Phosphatidylethanolamine methyltransferase (PEMT), catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis Gene:CLA4(YNL298W)|FD-Score:-3.94|P-value:4.07E-5||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CLN2(YPL256C)|FD-Score:-3.54|P-value:1.98E-4||SGD DESC:G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) Gene:DAP2(YHR028C)|FD-Score:5.03|P-value:2.47E-7||SGD DESC:Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p Gene:DIA3(YDL024C)|FD-Score:7.15|P-value:4.30E-13||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth Gene:EMC3(YKL207W)|FD-Score:-3.25|P-value:5.81E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; required for respiratory growth; null mutant displays induction of the unfolded protein response; homologous to worm Y62E10A.10/EMC-3, fly CG6750, human TMEM111 Gene:EMP24(YGL200C)|FD-Score:-3.14|P-value:8.35E-4||SGD DESC:Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles Gene:EOS1(YNL080C)|FD-Score:4.1|P-value:2.05E-5||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:ETR1(YBR026C)|FD-Score:3.79|P-value:7.38E-5||SGD DESC:2-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis Gene:FMP27(YLR454W_p)|FD-Score:3.92|P-value:4.50E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRE6(YLL051C)|FD-Score:-3.51|P-value:2.20E-4||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels Gene:FRT1(YOR324C)|FD-Score:-3.13|P-value:8.71E-4||SGD DESC:Tail-anchored ER membrane protein of unknown function; substrate of the phosphatase calcineurin; interacts with homolog Frt2p; promotes cell growth in stress conditions, possibly via a role in posttranslational translocation; FRT1 has a paralog, FRT2, that arose from the whole genome duplication Gene:GCY1(YOR120W)|FD-Score:3.13|P-value:8.61E-4||SGD DESC:Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication Gene:GEP4(YHR100C)|FD-Score:-3.39|P-value:3.45E-4||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GRX1(YCL035C)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Glutathione-dependent disulfide oxidoreductase; hydroperoxide and superoxide-radical responsive, heat-stable, with active site cysteine pair; protects cells from oxidative damage; GRX1 has a paralog, GRX2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:HHO1(YPL127C)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Histone H1, linker histone with roles in meiosis and sporulation; decreasing levels early in sporulation may promote meiosis, and increasing levels during sporulation facilitate compaction of spore chromatin; binds to promoters and within genes in mature spores; may be recruited by Ume6p to promoter regions, contributing to transcriptional repression outside of meiosis; suppresses DNA repair involving homologous recombination Gene:INO1(YJL153C)|FD-Score:5.82|P-value:2.94E-9||SGD DESC:Inositol-3-phosphate synthase, involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element Gene:LOC1(YFR001W)|FD-Score:3.39|P-value:3.56E-4||SGD DESC:Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:LYS14(YDR034C)|FD-Score:-3.21|P-value:6.55E-4||SGD DESC:Transcriptional activator involved in regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer Gene:MID2(YLR332W)|FD-Score:3.15|P-value:8.09E-4||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:MRPL23(YOR150W)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NCS2(YNL119W)|FD-Score:-3.32|P-value:4.49E-4||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:OGG1(YML060W)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance Gene:PFK27(YOL136C)|FD-Score:4.27|P-value:9.62E-6||SGD DESC:6-phosphofructo-2-kinase, catalyzes synthesis of fructose-2,6-bisphosphate; inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate, expression induced by glucose and sucrose, transcriptional regulation involves protein kinase A Gene:PTH2(YBL057C)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:QRI5(YLR204W)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:REX2(YLR059C)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease Gene:RGS2(YOR107W)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p Gene:RIM101(YHL027W)|FD-Score:3.11|P-value:9.22E-4||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:ROM2(YLR371W)|FD-Score:4.62|P-value:1.92E-6||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:SAC1(YKL212W)|FD-Score:6.4|P-value:7.67E-11||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SHU1(YHL006C)|FD-Score:-4.42|P-value:4.83E-6||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SHU2(YDR078C)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SNF2(YOR290C)|FD-Score:-3.38|P-value:3.59E-4||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SPT23(YKL020C)|FD-Score:-3.26|P-value:5.62E-4||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:STI1(YOR027W)|FD-Score:3.76|P-value:8.53E-5||SGD DESC:Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop Gene:STP2(YHR006W)|FD-Score:-4.23|P-value:1.15E-5||SGD DESC:Transcription factor; activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes; STP2 has a paralog, STP1, that arose from the whole genome duplication Gene:SVL3(YPL032C)|FD-Score:4.27|P-value:9.66E-6||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:TVP38(YKR088C)|FD-Score:5.2|P-value:9.73E-8||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:VID22(YLR373C)|FD-Score:-3.1|P-value:9.56E-4||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:YAP1802(YGR241C)|FD-Score:4.37|P-value:6.24E-6||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication Gene:YDC1(YPL087W)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Alkaline dihydroceramidase, involved in sphingolipid metabolism; preferentially hydrolyzes dihydroceramide to a free fatty acid and dihydrosphingosine; has a minor reverse activity; YDC1 has a paralog, YPC1, that arose from the whole genome duplication Gene:YDL026W(YDL026W_d)|FD-Score:-3.31|P-value:4.68E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER130C(YER130C_p)|FD-Score:4.3|P-value:8.58E-6||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YFL019C(YFL019C_d)|FD-Score:3.18|P-value:7.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Gene:YGR064W(YGR064W_d)|FD-Score:-3.29|P-value:5.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YIR020W-A(YIR020W-A_d)|FD-Score:4.38|P-value:6.05E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YLR374C(YLR374C_d)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W Gene:YMR007W(YMR007W_d)|FD-Score:-3.54|P-value:1.98E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR057C(YMR057C_d)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF AAC1 Gene:YMR130W(YMR130W_p)|FD-Score:-3.12|P-value:9.17E-4||SGD DESC:Putative protein of unknown function; YMR130W is not an essential gene Gene:YNL319W(YNL319W_d)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HXT14 Gene:YNR066C(YNR066C_p)|FD-Score:3.72|P-value:9.88E-5||SGD DESC:Putative membrane-localized protein of unknown function Gene:YOL013W-A(YOL013W-A_p)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YOL019W(YOL019W_p)|FD-Score:4.46|P-value:4.04E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; YOL019W has a paralog, DCV1, that arose from the whole genome duplication Gene:YPL041C(YPL041C_p)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Protein of unknown function involved in maintenance of proper telomere length Gene:YPL260W(YPL260W_p)|FD-Score:3.25|P-value:5.67E-4||SGD DESC:Putative substrate of cAMP-dependent protein kinase (PKA); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YPL260W is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YPR039W(YPR039W_d)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W Gene:YPR099C(YPR099C_d)|FD-Score:3.27|P-value:5.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL51/YPR100W

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPL028W4.582.36E-60.50ERG10Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis
YOL144W4.327.82E-60.50NOP8Nucleolar protein required for 60S ribosomal subunit biogenesis
YGL092W3.826.67E-50.27NUP145Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98
YGL030W3.551.94E-40.21RPL30Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog
YNL267W3.344.22E-40.11PIK1Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
YPR177C_d3.236.14E-40.21YPR177C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex
YLR163C3.020.001270.12MAS1Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
YDR168W2.900.001860.17CDC37Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding
YGR047C2.730.003140.07TFC4One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102
YPR108W2.660.003850.12RPN7Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits
YKL078W2.540.005530.04DHR2Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis
YKL125W2.500.006210.01RRN3Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo
YLR274W2.490.006330.06MCM5Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase
YGR048W2.430.007520.03UFD1Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP)
YPL016W2.400.008100.03SWI1Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDL024C7.154.30E-13DIA3Protein of unknown function, involved in invasive and pseudohyphal growth
YKL212W6.407.67E-11SAC1Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism
YJL153C5.822.94E-9INO1Inositol-3-phosphate synthase, involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element
YKR088C5.209.73E-8TVP38Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern
YHR028C5.032.47E-7DAP2Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p
YLR371W4.621.92E-6ROM2GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p
YOR377W4.592.19E-6ATF1Alcohol acetyltransferase; responsible for the major part of volatile acetate ester production during fermentation; main enzyme involved in terpenyl acetate synthesis; potential roles in lipid and sterol metabolism
YOL019W_p4.464.04E-6YOL019W_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; YOL019W has a paralog, DCV1, that arose from the whole genome duplication
YIR020W-A_d4.386.05E-6YIR020W-A_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data
YGR241C4.376.24E-6YAP1802Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication
YER130C_p4.308.58E-6YER130C_pProtein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress
YOL136C4.279.62E-6PFK276-phosphofructo-2-kinase, catalyzes synthesis of fructose-2,6-bisphosphate; inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate, expression induced by glucose and sucrose, transcriptional regulation involves protein kinase A
YPL032C4.279.66E-6SVL3Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress
YCL035C4.171.52E-5GRX1Glutathione-dependent disulfide oxidoreductase; hydroperoxide and superoxide-radical responsive, heat-stable, with active site cysteine pair; protects cells from oxidative damage; GRX1 has a paralog, GRX2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
YNL080C4.102.05E-5EOS1Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene

GO enrichment analysis for SGTC_2868
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0791.33E-9SGTC_830170-0301 39.0 μMChemDiv (Drug-like library)30897160.0606061endomembrane recycling
0.0782.28E-9SGTC_8110416-0114 265.0 μMChemDiv (Drug-like library)66124530.0821918
0.0723.09E-8SGTC_21836420625 130.5 μMChembridge (Fragment library)28976190.105263mitochondrial processes
0.0682.03E-7SGTC_1670st016595 4.0 μMTimTec (Natural product derivative library)5978790.0675676
0.0672.97E-7SGTC_1626st003712 1.8 μMTimTec (Natural product derivative library)242070350.175824
0.0673.15E-7SGTC_33099129340 25.8 μMChembridge (Drug-like library)171589870.168831
0.0655.95E-7SGTC_11513909-8316 15.3 μMChemDiv (Drug-like library)8004310.103896
0.0631.05E-6SGTC_31409094243 49.5 μMChembridge (Drug-like library)192911120.323529
0.0631.39E-6SGTC_1874staurosporine 7.6 μMICCB bioactive library468641930.0808081cell wall signaling
0.0621.96E-6SGTC_22287354201 200.0 μMChembridge (Fragment library)7534510.144928endomembrane recycling
0.0622.24E-6SGTC_437s-farnesyl-l-cysteine methyl ester 6.7 μMICCB bioactive library64392250.0649351
0.0612.93E-6SGTC_9691313-0238 134.0 μMChemDiv (Drug-like library)52128740.142857mitochondrial processes
0.0613.05E-6SGTC_22577960413 200.0 μMChembridge (Fragment library)29746890.0909091NEO1-PIK1
0.0603.36E-6SGTC_22027226349 200.0 μMChembridge (Fragment library)8443340.130435
0.0604.76E-6SGTC_11453546-0597 10.2 μMChemDiv (Drug-like library)8753340.0897436mitochondrial processes

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_2170morpholine200 μM0.462963121171Chembridge (Fragment library)234.294221.09303
SGTC_3049909222349.47 μM0.3650798623917Chembridge (Drug-like library)328.835843.93502
SGTC_7294092-058594.1 μM0.3636362169061ChemDiv (Drug-like library)390.4500234.5504
SGTC_13752910-0797186 μM0.349206742508ChemDiv (Drug-like library)340.416162.75204
SGTC_3158910000749.47 μM0.34920619297233Chembridge (Drug-like library)373.286844.01902
SGTC_2931901467455.67 μM0.3281252996810Chembridge (Drug-like library)326.174624.03323
SGTC_2929901334824.6 μM0.3278692995952Chembridge (Drug-like library)310.175223.99912
SGTC_1613st00238152.1 μM0.323944551167TimTec (Natural product derivative library)363.45283.83913
SGTC_3140909424349.47 μM0.32352919291112Chembridge (Drug-like library)342.4072233.32204
SGTC_11564092-03674.88 μM0.3181821357585ChemDiv (Drug-like library)382.926285.00702