9038510

N-(3-methoxyphenyl)-4-prop-2-enoxybenzamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2871
Screen concentration 13.0 μM
Source Chembridge (Drug-like library)
PubChem CID 4945164
SMILES COC1=CC=CC(=C1)NC(=O)C2=CC=C(C=C2)OCC=C
Standardized SMILES COc1cccc(NC(=O)c2ccc(OCC=C)cc2)c1
Molecular weight 283.3218
ALogP 3.2
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.19
% growth inhibition (Hom. pool) 5.26


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4945164
Download HIP data (tab-delimited text)  (excel)
Gene:ERG12(YMR208W)|FD-Score:-3.1|P-value:9.64E-4|Clearance:0||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:GPI11(YDR302W)|FD-Score:3.14|P-value:8.45E-4|Clearance:0.06||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:HSP10(YOR020C)|FD-Score:5.24|P-value:8.21E-8|Clearance:1.24||SGD DESC:Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES Gene:ILV5(YLR355C)|FD-Score:3.62|P-value:1.49E-4|Clearance:0.26||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:IRA1(YBR140C)|FD-Score:-3.09|P-value:9.95E-4|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:MCM5(YLR274W)|FD-Score:3.35|P-value:4.09E-4|Clearance:0.04||SGD DESC:Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase Gene:NOC3(YLR002C)|FD-Score:3.26|P-value:5.54E-4|Clearance:0.06||SGD DESC:Component of a nuclear complex with Noc2p and pre-replicative complexes; the Noc2p-Noc3p complex binds to 66S ribosomal precursors to mediate their maturation and intranuclear transport; binds to chromatin at active replication origins, and is required for pre-RC formation and maintenance during DNA replication licensing Gene:OST1(YJL002C)|FD-Score:5.92|P-value:1.62E-9|Clearance:1.24||SGD DESC:Alpha subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:PBN1(YCL052C)|FD-Score:-3.13|P-value:8.79E-4|Clearance:0||SGD DESC:Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X Gene:RAD53(YPL153C)|FD-Score:3.75|P-value:8.80E-5|Clearance:0.13||SGD DESC:Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication Gene:RFC3(YNL290W)|FD-Score:3.77|P-value:8.22E-5|Clearance:0.02||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RPC31(YNL151C)|FD-Score:3.88|P-value:5.25E-5|Clearance:0.11||SGD DESC:RNA polymerase III subunit C31 Gene:RPF2(YKR081C)|FD-Score:4|P-value:3.17E-5|Clearance:0.12||SGD DESC:Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RPP1(YHR062C)|FD-Score:-4.5|P-value:3.46E-6|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SAS10(YDL153C)|FD-Score:-3.88|P-value:5.17E-5|Clearance:0||SGD DESC:Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance Gene:SPC19(YDR201W)|FD-Score:3.21|P-value:6.74E-4|Clearance:0.07||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body Gene:SPC98(YNL126W)|FD-Score:3.31|P-value:4.71E-4|Clearance:0.05||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:TFC8(YPL007C)|FD-Score:-3.39|P-value:3.49E-4|Clearance:0||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90 Gene:UBC1(YDR177W)|FD-Score:-4.81|P-value:7.40E-7|Clearance:0||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress Gene:YOR102W(YOR102W_d)|FD-Score:3.36|P-value:3.86E-4|Clearance:0.02||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex Gene:ERG12(YMR208W)|FD-Score:-3.1|P-value:9.64E-4|Clearance:0||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:GPI11(YDR302W)|FD-Score:3.14|P-value:8.45E-4|Clearance:0.06||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:HSP10(YOR020C)|FD-Score:5.24|P-value:8.21E-8|Clearance:1.24||SGD DESC:Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES Gene:ILV5(YLR355C)|FD-Score:3.62|P-value:1.49E-4|Clearance:0.26||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:IRA1(YBR140C)|FD-Score:-3.09|P-value:9.95E-4|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:MCM5(YLR274W)|FD-Score:3.35|P-value:4.09E-4|Clearance:0.04||SGD DESC:Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase Gene:NOC3(YLR002C)|FD-Score:3.26|P-value:5.54E-4|Clearance:0.06||SGD DESC:Component of a nuclear complex with Noc2p and pre-replicative complexes; the Noc2p-Noc3p complex binds to 66S ribosomal precursors to mediate their maturation and intranuclear transport; binds to chromatin at active replication origins, and is required for pre-RC formation and maintenance during DNA replication licensing Gene:OST1(YJL002C)|FD-Score:5.92|P-value:1.62E-9|Clearance:1.24||SGD DESC:Alpha subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:PBN1(YCL052C)|FD-Score:-3.13|P-value:8.79E-4|Clearance:0||SGD DESC:Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X Gene:RAD53(YPL153C)|FD-Score:3.75|P-value:8.80E-5|Clearance:0.13||SGD DESC:Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication Gene:RFC3(YNL290W)|FD-Score:3.77|P-value:8.22E-5|Clearance:0.02||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RPC31(YNL151C)|FD-Score:3.88|P-value:5.25E-5|Clearance:0.11||SGD DESC:RNA polymerase III subunit C31 Gene:RPF2(YKR081C)|FD-Score:4|P-value:3.17E-5|Clearance:0.12||SGD DESC:Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RPP1(YHR062C)|FD-Score:-4.5|P-value:3.46E-6|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SAS10(YDL153C)|FD-Score:-3.88|P-value:5.17E-5|Clearance:0||SGD DESC:Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance Gene:SPC19(YDR201W)|FD-Score:3.21|P-value:6.74E-4|Clearance:0.07||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body Gene:SPC98(YNL126W)|FD-Score:3.31|P-value:4.71E-4|Clearance:0.05||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:TFC8(YPL007C)|FD-Score:-3.39|P-value:3.49E-4|Clearance:0||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90 Gene:UBC1(YDR177W)|FD-Score:-4.81|P-value:7.40E-7|Clearance:0||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress Gene:YOR102W(YOR102W_d)|FD-Score:3.36|P-value:3.86E-4|Clearance:0.02||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4945164
Download HOP data (tab-delimited text)  (excel)
Gene:AIR1(YIL079C)|FD-Score:-3.23|P-value:6.13E-4||SGD DESC:Zinc knuckle protein; involved in nuclear RNA processing and degradation as a component of the TRAMP complex; stimulates the poly(A) polymerase activity of Pap2p in vitro; AIR1 has a paralog, AIR2, that arose from the whole genome duplication Gene:ALG8(YOR067C)|FD-Score:3.78|P-value:7.74E-5||SGD DESC:Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p Gene:ARP6(YLR085C)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:ATG18(YFR021W)|FD-Score:-3.51|P-value:2.21E-4||SGD DESC:Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood Gene:ATP2(YJR121W)|FD-Score:5.65|P-value:8.18E-9||SGD DESC:Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:BAP3(YDR046C)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Amino acid permease; involved in uptake of cysteine, leucine, isoleucine and valine; BAP3 has a paralog, BAP2, that arose from the whole genome duplication Gene:BIO5(YNR056C)|FD-Score:3.2|P-value:6.98E-4||SGD DESC:Putative transmembrane protein involved in the biotin biosynthesis pathway; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:CDC55(YGL190C)|FD-Score:-3.69|P-value:1.14E-4||SGD DESC:Non-essential regulatory subunit B of protein phosphatase 2A (PP2A); localization to the cytoplasm requires Zds1p and Zds2p and promotes mitotic entry; localization to the nucleus prevents mitotic exit; required for correct nuclear division and chromosome segregation in meiosis; maintains nucleolar sequestration of Cdc14p during early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion Gene:CHO1(YER026C)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline Gene:CHZ1(YER030W)|FD-Score:3.13|P-value:8.72E-4||SGD DESC:Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif; protein abundance increases in response to DNA replication stress Gene:CLA4(YNL298W)|FD-Score:-3.12|P-value:9.17E-4||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:COG6(YNL041C)|FD-Score:3.17|P-value:7.69E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CWH43(YCR017C)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:DIT1(YDR403W)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Sporulation-specific enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure Gene:DOS2(YDR068W)|FD-Score:4.99|P-value:3.04E-7||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:DUR1,2(YBR208C)|FD-Score:3.16|P-value:7.81E-4||SGD DESC:Urea amidolyase; contains both urea carboxylase and allophanate hydrolase activities, degrades urea to CO2 and NH3; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation; protein abundance increases in response to DNA replication stress Gene:EDE1(YBL047C)|FD-Score:-3.51|P-value:2.27E-4||SGD DESC:Endocytic protein; involved in a network of interactions with other endocytic proteins, binds membranes in a ubiquitin-dependent manner, may also bind ubiquitinated membrane-associated proteins; interacts with Cmk2 and functions upstream of CMK2 in regulating non-apoptotic cell death Gene:FAT3(YKL187C_p)|FD-Score:3.15|P-value:8.30E-4||SGD DESC:Protein required for fatty acid uptake; Fat3p abundance increases in cortical patches in response to oleate exposure; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies Gene:FLC2(YAL053W)|FD-Score:-4.22|P-value:1.20E-5||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication Gene:FUR4(YBR021W)|FD-Score:-3.34|P-value:4.20E-4||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:FYV6(YNL133C)|FD-Score:-5.11|P-value:1.64E-7||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:GDE1(YPL110C)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes Gene:GIM5(YML094W)|FD-Score:-3.15|P-value:8.25E-4||SGD DESC:Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it Gene:GPD1(YDL022W)|FD-Score:5.35|P-value:4.30E-8||SGD DESC:NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity Gene:GUP1(YGL084C)|FD-Score:-4.32|P-value:7.80E-6||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:ICP55(YER078C)|FD-Score:-3.23|P-value:6.25E-4||SGD DESC:Mitochondrial aminopeptidase; cleaves the N termini of at least 38 imported proteins after cleavage by the mitochondrial processing peptidase (MPP), thereby increasing their stability; member of the aminopeptidase P family Gene:IRS4(YKR019C)|FD-Score:3.82|P-value:6.56E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:JEM1(YJL073W)|FD-Score:3.19|P-value:6.99E-4||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:KXD1(YGL079W_p)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome Gene:LIP5(YOR196C)|FD-Score:3.79|P-value:7.65E-5||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:LYS21(YDL131W)|FD-Score:3.91|P-value:4.70E-5||SGD DESC:Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS21 has a paralog, LYS20, that arose from the whole genome duplication Gene:MEP1(YGR121C)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Ammonium permease; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MF(ALPHA)2(YGL089C)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Mating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)1, which is more highly expressed than MF(ALPHA)2 Gene:MFM1(YPL060W)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p Gene:MMS22(YLR320W)|FD-Score:-3.11|P-value:9.27E-4||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation Gene:MSE1(YOL033W)|FD-Score:-4.68|P-value:1.44E-6||SGD DESC:Mitochondrial glutamyl-tRNA synthetase, predicted to be palmitoylated Gene:MTG1(YMR097C)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:NCL1(YBL024W)|FD-Score:3.73|P-value:9.72E-5||SGD DESC:S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 Gene:NIT3(YLR351C)|FD-Score:3.87|P-value:5.53E-5||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:NKP1(YDR383C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein fta4 Gene:NSG1(YHR133C)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins; forms foci at the nuclear periphery upon DNA replication stress Gene:OCA1(YNL099C)|FD-Score:4.58|P-value:2.36E-6||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:PDR11(YIL013C)|FD-Score:3.78|P-value:7.86E-5||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth Gene:PHO84(YML123C)|FD-Score:-4.07|P-value:2.33E-5||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PRE9(YGR135W)|FD-Score:3.99|P-value:3.35E-5||SGD DESC:Alpha 3 subunit of the 20S proteasome, the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform Gene:REV1(YOR346W)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RGS2(YOR107W)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p Gene:RNP1(YLL046C)|FD-Score:3.88|P-value:5.26E-5||SGD DESC:Ribonucleoprotein that contains two RNA recognition motifs (RRM) Gene:RPL31A(YDL075W)|FD-Score:-3.37|P-value:3.75E-4||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:4.82|P-value:7.25E-7||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTT107(YHR154W)|FD-Score:-3.39|P-value:3.46E-4||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SDC1(YDR469W)|FD-Score:-4.85|P-value:6.07E-7||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 Gene:SNF2(YOR290C)|FD-Score:-3.59|P-value:1.67E-4||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SPG1(YGR236C)|FD-Score:5.88|P-value:2.10E-9||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SWI6(YLR182W)|FD-Score:-4.39|P-value:5.56E-6||SGD DESC:Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators Gene:SXM1(YDR395W)|FD-Score:4.17|P-value:1.49E-5||SGD DESC:Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1 Gene:SYF2(YGR129W)|FD-Score:-3.35|P-value:4.10E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TEA1(YOR337W)|FD-Score:4.04|P-value:2.68E-5||SGD DESC:Ty1 enhancer activator required for full levels of Ty enhancer-mediated transcription; C6 zinc cluster DNA-binding protein Gene:TRK1(YJL129C)|FD-Score:-3.28|P-value:5.14E-4||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:TRP3(YKL211C)|FD-Score:4.88|P-value:5.37E-7||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UGA3(YDL170W)|FD-Score:-4.05|P-value:2.54E-5||SGD DESC:Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus Gene:YAP6(YDR259C)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; overexpression increases sodium and lithium tolerance; computational analysis suggests a role in regulation of expression of genes involved in carbohydrate metabolism; YAP6 has a paralog, CIN5, that arose from the whole genome duplication Gene:YBL008W-A(YBL008W-A_p)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YCR022C(YCR022C_d)|FD-Score:3.91|P-value:4.68E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR022C is not an essential gene Gene:YDL023C(YDL023C_d)|FD-Score:3.72|P-value:9.98E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in other Saccharomyces species; overlaps the verified gene GPD1; deletion confers sensitivity to GSAO; deletion in cyr1 mutant results in loss of stress resistance Gene:YDR008C(YDR008C_d)|FD-Score:5.38|P-value:3.75E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR535C(YDR535C_d)|FD-Score:5.52|P-value:1.71E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Gene:YFR006W(YFR006W_p)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene Gene:YGL262W(YGL262W_p)|FD-Score:3.13|P-value:8.73E-4||SGD DESC:Putative protein of unknown function; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein; YGL262W is not an essential gene Gene:YGR130C(YGR130C)|FD-Score:-3.32|P-value:4.45E-4||SGD DESC:Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) Gene:YLR184W(YLR184W_d)|FD-Score:4.57|P-value:2.44E-6||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR031W-A(YMR031W-A_d)|FD-Score:3.85|P-value:5.98E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Gene:YMR085W(YMR085W_p)|FD-Score:-3.34|P-value:4.23E-4||SGD DESC:Putative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YMR304C-A(YMR304C-A_d)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SCW10 Gene:YNL108C(YNL108C_p)|FD-Score:3.77|P-value:8.12E-5||SGD DESC:Putative protein of unknown function; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication Gene:YNL143C(YNL143C_p)|FD-Score:-3.22|P-value:6.38E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOR318C(YOR318C_d)|FD-Score:3.88|P-value:5.28E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo Gene:YOR333C(YOR333C_d)|FD-Score:3.96|P-value:3.75E-5||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; overlaps 5' end of MRS2 gene required for respiratory growth Gene:YOR385W(YOR385W_p)|FD-Score:4.28|P-value:9.52E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:AIR1(YIL079C)|FD-Score:-3.23|P-value:6.13E-4||SGD DESC:Zinc knuckle protein; involved in nuclear RNA processing and degradation as a component of the TRAMP complex; stimulates the poly(A) polymerase activity of Pap2p in vitro; AIR1 has a paralog, AIR2, that arose from the whole genome duplication Gene:ALG8(YOR067C)|FD-Score:3.78|P-value:7.74E-5||SGD DESC:Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p Gene:ARP6(YLR085C)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:ATG18(YFR021W)|FD-Score:-3.51|P-value:2.21E-4||SGD DESC:Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood Gene:ATP2(YJR121W)|FD-Score:5.65|P-value:8.18E-9||SGD DESC:Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:BAP3(YDR046C)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Amino acid permease; involved in uptake of cysteine, leucine, isoleucine and valine; BAP3 has a paralog, BAP2, that arose from the whole genome duplication Gene:BIO5(YNR056C)|FD-Score:3.2|P-value:6.98E-4||SGD DESC:Putative transmembrane protein involved in the biotin biosynthesis pathway; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:CDC55(YGL190C)|FD-Score:-3.69|P-value:1.14E-4||SGD DESC:Non-essential regulatory subunit B of protein phosphatase 2A (PP2A); localization to the cytoplasm requires Zds1p and Zds2p and promotes mitotic entry; localization to the nucleus prevents mitotic exit; required for correct nuclear division and chromosome segregation in meiosis; maintains nucleolar sequestration of Cdc14p during early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion Gene:CHO1(YER026C)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline Gene:CHZ1(YER030W)|FD-Score:3.13|P-value:8.72E-4||SGD DESC:Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif; protein abundance increases in response to DNA replication stress Gene:CLA4(YNL298W)|FD-Score:-3.12|P-value:9.17E-4||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:COG6(YNL041C)|FD-Score:3.17|P-value:7.69E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CWH43(YCR017C)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:DIT1(YDR403W)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Sporulation-specific enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure Gene:DOS2(YDR068W)|FD-Score:4.99|P-value:3.04E-7||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:DUR1,2(YBR208C)|FD-Score:3.16|P-value:7.81E-4||SGD DESC:Urea amidolyase; contains both urea carboxylase and allophanate hydrolase activities, degrades urea to CO2 and NH3; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation; protein abundance increases in response to DNA replication stress Gene:EDE1(YBL047C)|FD-Score:-3.51|P-value:2.27E-4||SGD DESC:Endocytic protein; involved in a network of interactions with other endocytic proteins, binds membranes in a ubiquitin-dependent manner, may also bind ubiquitinated membrane-associated proteins; interacts with Cmk2 and functions upstream of CMK2 in regulating non-apoptotic cell death Gene:FAT3(YKL187C_p)|FD-Score:3.15|P-value:8.30E-4||SGD DESC:Protein required for fatty acid uptake; Fat3p abundance increases in cortical patches in response to oleate exposure; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies Gene:FLC2(YAL053W)|FD-Score:-4.22|P-value:1.20E-5||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication Gene:FUR4(YBR021W)|FD-Score:-3.34|P-value:4.20E-4||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:FYV6(YNL133C)|FD-Score:-5.11|P-value:1.64E-7||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:GDE1(YPL110C)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes Gene:GIM5(YML094W)|FD-Score:-3.15|P-value:8.25E-4||SGD DESC:Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it Gene:GPD1(YDL022W)|FD-Score:5.35|P-value:4.30E-8||SGD DESC:NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity Gene:GUP1(YGL084C)|FD-Score:-4.32|P-value:7.80E-6||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:ICP55(YER078C)|FD-Score:-3.23|P-value:6.25E-4||SGD DESC:Mitochondrial aminopeptidase; cleaves the N termini of at least 38 imported proteins after cleavage by the mitochondrial processing peptidase (MPP), thereby increasing their stability; member of the aminopeptidase P family Gene:IRS4(YKR019C)|FD-Score:3.82|P-value:6.56E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:JEM1(YJL073W)|FD-Score:3.19|P-value:6.99E-4||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:KXD1(YGL079W_p)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome Gene:LIP5(YOR196C)|FD-Score:3.79|P-value:7.65E-5||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:LYS21(YDL131W)|FD-Score:3.91|P-value:4.70E-5||SGD DESC:Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS21 has a paralog, LYS20, that arose from the whole genome duplication Gene:MEP1(YGR121C)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Ammonium permease; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MF(ALPHA)2(YGL089C)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Mating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)1, which is more highly expressed than MF(ALPHA)2 Gene:MFM1(YPL060W)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p Gene:MMS22(YLR320W)|FD-Score:-3.11|P-value:9.27E-4||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation Gene:MSE1(YOL033W)|FD-Score:-4.68|P-value:1.44E-6||SGD DESC:Mitochondrial glutamyl-tRNA synthetase, predicted to be palmitoylated Gene:MTG1(YMR097C)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:NCL1(YBL024W)|FD-Score:3.73|P-value:9.72E-5||SGD DESC:S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 Gene:NIT3(YLR351C)|FD-Score:3.87|P-value:5.53E-5||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:NKP1(YDR383C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein fta4 Gene:NSG1(YHR133C)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins; forms foci at the nuclear periphery upon DNA replication stress Gene:OCA1(YNL099C)|FD-Score:4.58|P-value:2.36E-6||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:PDR11(YIL013C)|FD-Score:3.78|P-value:7.86E-5||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth Gene:PHO84(YML123C)|FD-Score:-4.07|P-value:2.33E-5||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PRE9(YGR135W)|FD-Score:3.99|P-value:3.35E-5||SGD DESC:Alpha 3 subunit of the 20S proteasome, the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform Gene:REV1(YOR346W)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RGS2(YOR107W)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p Gene:RNP1(YLL046C)|FD-Score:3.88|P-value:5.26E-5||SGD DESC:Ribonucleoprotein that contains two RNA recognition motifs (RRM) Gene:RPL31A(YDL075W)|FD-Score:-3.37|P-value:3.75E-4||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:4.82|P-value:7.25E-7||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTT107(YHR154W)|FD-Score:-3.39|P-value:3.46E-4||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SDC1(YDR469W)|FD-Score:-4.85|P-value:6.07E-7||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 Gene:SNF2(YOR290C)|FD-Score:-3.59|P-value:1.67E-4||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SPG1(YGR236C)|FD-Score:5.88|P-value:2.10E-9||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SWI6(YLR182W)|FD-Score:-4.39|P-value:5.56E-6||SGD DESC:Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators Gene:SXM1(YDR395W)|FD-Score:4.17|P-value:1.49E-5||SGD DESC:Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1 Gene:SYF2(YGR129W)|FD-Score:-3.35|P-value:4.10E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TEA1(YOR337W)|FD-Score:4.04|P-value:2.68E-5||SGD DESC:Ty1 enhancer activator required for full levels of Ty enhancer-mediated transcription; C6 zinc cluster DNA-binding protein Gene:TRK1(YJL129C)|FD-Score:-3.28|P-value:5.14E-4||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:TRP3(YKL211C)|FD-Score:4.88|P-value:5.37E-7||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UGA3(YDL170W)|FD-Score:-4.05|P-value:2.54E-5||SGD DESC:Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus Gene:YAP6(YDR259C)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; overexpression increases sodium and lithium tolerance; computational analysis suggests a role in regulation of expression of genes involved in carbohydrate metabolism; YAP6 has a paralog, CIN5, that arose from the whole genome duplication Gene:YBL008W-A(YBL008W-A_p)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YCR022C(YCR022C_d)|FD-Score:3.91|P-value:4.68E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR022C is not an essential gene Gene:YDL023C(YDL023C_d)|FD-Score:3.72|P-value:9.98E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in other Saccharomyces species; overlaps the verified gene GPD1; deletion confers sensitivity to GSAO; deletion in cyr1 mutant results in loss of stress resistance Gene:YDR008C(YDR008C_d)|FD-Score:5.38|P-value:3.75E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR535C(YDR535C_d)|FD-Score:5.52|P-value:1.71E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Gene:YFR006W(YFR006W_p)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene Gene:YGL262W(YGL262W_p)|FD-Score:3.13|P-value:8.73E-4||SGD DESC:Putative protein of unknown function; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein; YGL262W is not an essential gene Gene:YGR130C(YGR130C)|FD-Score:-3.32|P-value:4.45E-4||SGD DESC:Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) Gene:YLR184W(YLR184W_d)|FD-Score:4.57|P-value:2.44E-6||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR031W-A(YMR031W-A_d)|FD-Score:3.85|P-value:5.98E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Gene:YMR085W(YMR085W_p)|FD-Score:-3.34|P-value:4.23E-4||SGD DESC:Putative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YMR304C-A(YMR304C-A_d)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SCW10 Gene:YNL108C(YNL108C_p)|FD-Score:3.77|P-value:8.12E-5||SGD DESC:Putative protein of unknown function; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication Gene:YNL143C(YNL143C_p)|FD-Score:-3.22|P-value:6.38E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOR318C(YOR318C_d)|FD-Score:3.88|P-value:5.28E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo Gene:YOR333C(YOR333C_d)|FD-Score:3.96|P-value:3.75E-5||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; overlaps 5' end of MRS2 gene required for respiratory growth Gene:YOR385W(YOR385W_p)|FD-Score:4.28|P-value:9.52E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJL002C5.921.62E-91.24OST1Alpha subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins
YOR020C5.248.21E-81.24HSP10Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES
YKR081C4.003.17E-50.12RPF2Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles
YNL151C3.885.25E-50.11RPC31RNA polymerase III subunit C31
YNL290W3.778.22E-50.02RFC3Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YPL153C3.758.80E-50.13RAD53Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication
YLR355C3.621.49E-40.26ILV5Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids
YOR102W_d3.363.86E-40.02YOR102W_dDubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex
YLR274W3.354.09E-40.04MCM5Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase
YNL126W3.314.71E-40.05SPC98Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
YLR002C3.265.54E-40.06NOC3Component of a nuclear complex with Noc2p and pre-replicative complexes; the Noc2p-Noc3p complex binds to 66S ribosomal precursors to mediate their maturation and intranuclear transport; binds to chromatin at active replication origins, and is required for pre-RC formation and maintenance during DNA replication licensing
YDR201W3.216.74E-40.07SPC19Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body
YDR302W3.148.45E-40.06GPI11ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog
YLR314C3.080.001040.05CDC3Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YKL196C3.030.001220.04YKT6Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGR236C5.882.10E-9SPG1Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YJR121W5.658.18E-9ATP2Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated
YDR535C_d5.521.71E-8YDR535C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene.
YDR008C_d5.383.75E-8YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YDL022W5.354.30E-8GPD1NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity
YDR068W4.993.04E-7DOS2Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YKL211C4.885.37E-7TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YDL020C4.827.25E-7RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YNL099C4.582.36E-6OCA1Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA
YLR184W_d4.572.44E-6YLR184W_dDubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
YOR385W_p4.289.52E-6YOR385W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene
YDR395W4.171.49E-5SXM1Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1
YOR337W4.042.68E-5TEA1Ty1 enhancer activator required for full levels of Ty enhancer-mediated transcription; C6 zinc cluster DNA-binding protein
YGR135W3.993.35E-5PRE9Alpha 3 subunit of the 20S proteasome, the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform
YOR333C_d3.963.75E-5YOR333C_dDubious open reading frame, unlikely to encode a functional protein; overlaps 5' end of MRS2 gene required for respiratory growth

GO enrichment analysis for SGTC_2871
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1313.82E-24SGTC_21976588183 93.1 μMChembridge (Fragment library)8841360.171429
0.1211.13E-20SGTC_2231micatex 6.9 μMMiscellaneous207370.0714286
0.1202.52E-20SGTC_22246650123 117.6 μMChembridge (Fragment library)29083700.123288
0.1072.28E-16SGTC_21945927483 104.5 μMChembridge (Fragment library)7609790.147541
0.1023.27E-15SGTC_2683piperine 63.0 μMTimTec (Pure natural product library)6380240.111111
0.1023.98E-15SGTC_1975st070967 30.8 μMTimTec (Natural product derivative library)2613910.0757576
0.0984.31E-14SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.166667
0.0984.32E-14SGTC_1801486-1293 102.8 μMChemDiv (Drug-like library)41405360.202703
0.0971.03E-13SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0862069
0.0921.26E-12SGTC_29187977171 22.1 μMChembridge (Drug-like library)22364830.205882
0.0912.90E-12SGTC_7371950-0117 57.9 μMChemDiv (Drug-like library)53935130.148649
0.0898.49E-12SGTC_23127706453 155.7 μMChembridge (Fragment library)9760980.136986
0.0899.10E-12SGTC_1671st016596 74.5 μMTimTec (Natural product derivative library)28291670.102564
0.0881.28E-11SGTC_14740330-0125 5.3 μMChemDiv (Drug-like library)X14740.108108
0.0881.32E-11SGTC_2409alverine citrate 64.0 μMMiscellaneous217180.103448

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2827799384771.43 μM0.8222222985692Chembridge (Drug-like library)283.321783.19813
SGTC_2881905895351.95 μM0.67346917122249Chembridge (Drug-like library)281.30593.77413
SGTC_2867903909871.43 μM0.646463460Chembridge (Drug-like library)287.740863.87912iron homeostasis
SGTC_2926901072665.15 μM0.6252994293Chembridge (Drug-like library)253.29583.21512
SGTC_2999906976571.43 μM0.6252097066Chembridge (Drug-like library)285.337663.30813amide catabolism
SGTC_3077911731949.47 μM0.59183717154678Chembridge (Drug-like library)285.337663.30813
SGTC_3205911348349.47 μM0.5617018033Chembridge (Drug-like library)271.311083.06813
SGTC_2900532852885.29 μM0.55102795359Chembridge (Drug-like library)261.703583.26212Golgi
SGTC_2998906676271.43 μM0.547174945196Chembridge (Drug-like library)297.348363.54713RPP1 & pyrimidine depletion
SGTC_3091911637249.47 μM0.53846217169793Chembridge (Drug-like library)285.337662.95313