9040596

N-(5-ethyl-1,3,4-thiadiazol-2-yl)-1-(2-fluorophenyl)-5-methyltriazole-4-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2873
Screen concentration 71.4 μM
Source Chembridge (Drug-like library)
PubChem CID 6463863
SMILES CCC1=NN=C(S1)NC(=O)C2=C(N(N=N2)C3=CC=CC=C3F)C
Standardized SMILES CCc1nnc(NC(=O)c2nnn(c2C)c3ccccc3F)s1
Molecular weight 332.356
ALogP 2.55
H-bond donor count 1
H-bond acceptor count 7
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.55
% growth inhibition (Hom. pool) 5.42


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6463863
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:6.27|P-value:1.76E-10|Clearance:1.35||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:AME1(YBR211C)|FD-Score:3.6|P-value:1.57E-4|Clearance:0||SGD DESC:Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress Gene:ARP9(YMR033W)|FD-Score:3.6|P-value:1.58E-4|Clearance:0.04||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:DBP5(YOR046C)|FD-Score:3.26|P-value:5.52E-4|Clearance:0.07||SGD DESC:Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus; involved in translation termination; ATP/ADP cycling is regulated by Gle1p and Nup159p Gene:ECM16(YMR128W)|FD-Score:-4|P-value:3.21E-5|Clearance:0||SGD DESC:Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis Gene:MCM1(YMR043W)|FD-Score:3.43|P-value:3.07E-4|Clearance:0.05||SGD DESC:Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes Gene:MCM7(YBR202W)|FD-Score:-3.61|P-value:1.55E-4|Clearance:0||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:MEX67(YPL169C)|FD-Score:5.76|P-value:4.11E-9|Clearance:1.35||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:MSL5(YLR116W)|FD-Score:3.27|P-value:5.44E-4|Clearance:0||SGD DESC:Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA Gene:POB3(YML069W)|FD-Score:3.09|P-value:9.97E-4|Clearance:0.01||SGD DESC:Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; protein abundance increases in response to DNA replication stress Gene:PRE2(YPR103W)|FD-Score:3.17|P-value:7.74E-4|Clearance:0.07||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:RFA1(YAR007C)|FD-Score:4.41|P-value:5.19E-6|Clearance:0.6||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination Gene:RIO2(YNL207W)|FD-Score:-3.81|P-value:6.88E-5|Clearance:0||SGD DESC:Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p Gene:RPS20(YHL015W)|FD-Score:5.85|P-value:2.45E-9|Clearance:1.35||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RRP4(YHR069C)|FD-Score:3.37|P-value:3.79E-4|Clearance:0.1||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:SEC2(YNL272C)|FD-Score:3.77|P-value:8.29E-5|Clearance:0.05||SGD DESC:Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles Gene:SMD2(YLR275W)|FD-Score:3.81|P-value:6.98E-5|Clearance:0.04||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:SMT3(YDR510W)|FD-Score:-3.31|P-value:4.65E-4|Clearance:0||SGD DESC:Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2 Gene:SPN1(YPR133C)|FD-Score:3.56|P-value:1.82E-4|Clearance:0.14||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:TOA2(YKL058W)|FD-Score:3.37|P-value:3.69E-4|Clearance:0.01||SGD DESC:TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress Gene:TRR1(YDR353W)|FD-Score:3.72|P-value:1.02E-4|Clearance:0.11||SGD DESC:Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication Gene:UBA1(YKL210W)|FD-Score:3.2|P-value:6.95E-4|Clearance:0.03||SGD DESC:Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress Gene:YMR290W-A(YMR290W-A_d)|FD-Score:-3.92|P-value:4.45E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:ACT1(YFL039C)|FD-Score:6.27|P-value:1.76E-10|Clearance:1.35||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:AME1(YBR211C)|FD-Score:3.6|P-value:1.57E-4|Clearance:0||SGD DESC:Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress Gene:ARP9(YMR033W)|FD-Score:3.6|P-value:1.58E-4|Clearance:0.04||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:DBP5(YOR046C)|FD-Score:3.26|P-value:5.52E-4|Clearance:0.07||SGD DESC:Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus; involved in translation termination; ATP/ADP cycling is regulated by Gle1p and Nup159p Gene:ECM16(YMR128W)|FD-Score:-4|P-value:3.21E-5|Clearance:0||SGD DESC:Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis Gene:MCM1(YMR043W)|FD-Score:3.43|P-value:3.07E-4|Clearance:0.05||SGD DESC:Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes Gene:MCM7(YBR202W)|FD-Score:-3.61|P-value:1.55E-4|Clearance:0||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:MEX67(YPL169C)|FD-Score:5.76|P-value:4.11E-9|Clearance:1.35||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:MSL5(YLR116W)|FD-Score:3.27|P-value:5.44E-4|Clearance:0||SGD DESC:Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA Gene:POB3(YML069W)|FD-Score:3.09|P-value:9.97E-4|Clearance:0.01||SGD DESC:Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; protein abundance increases in response to DNA replication stress Gene:PRE2(YPR103W)|FD-Score:3.17|P-value:7.74E-4|Clearance:0.07||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:RFA1(YAR007C)|FD-Score:4.41|P-value:5.19E-6|Clearance:0.6||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination Gene:RIO2(YNL207W)|FD-Score:-3.81|P-value:6.88E-5|Clearance:0||SGD DESC:Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p Gene:RPS20(YHL015W)|FD-Score:5.85|P-value:2.45E-9|Clearance:1.35||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RRP4(YHR069C)|FD-Score:3.37|P-value:3.79E-4|Clearance:0.1||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:SEC2(YNL272C)|FD-Score:3.77|P-value:8.29E-5|Clearance:0.05||SGD DESC:Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles Gene:SMD2(YLR275W)|FD-Score:3.81|P-value:6.98E-5|Clearance:0.04||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:SMT3(YDR510W)|FD-Score:-3.31|P-value:4.65E-4|Clearance:0||SGD DESC:Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2 Gene:SPN1(YPR133C)|FD-Score:3.56|P-value:1.82E-4|Clearance:0.14||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:TOA2(YKL058W)|FD-Score:3.37|P-value:3.69E-4|Clearance:0.01||SGD DESC:TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress Gene:TRR1(YDR353W)|FD-Score:3.72|P-value:1.02E-4|Clearance:0.11||SGD DESC:Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication Gene:UBA1(YKL210W)|FD-Score:3.2|P-value:6.95E-4|Clearance:0.03||SGD DESC:Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress Gene:YMR290W-A(YMR290W-A_d)|FD-Score:-3.92|P-value:4.45E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6463863
Download HOP data (tab-delimited text)  (excel)
Gene:AFT1(YGL071W)|FD-Score:6.52|P-value:3.50E-11||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:APM4(YOL062C)|FD-Score:-3.24|P-value:5.92E-4||SGD DESC:Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport Gene:ARA1(YBR149W)|FD-Score:6.1|P-value:5.18E-10||SGD DESC:NADP+ dependent arabinose dehydrogenase, involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product Gene:ARC18(YLR370C)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARL1(YBR164C)|FD-Score:3.09|P-value:9.99E-4||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ARP6(YLR085C)|FD-Score:4.06|P-value:2.45E-5||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:ASI1(YMR119W)|FD-Score:3.96|P-value:3.73E-5||SGD DESC:Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication Gene:ATG18(YFR021W)|FD-Score:-3.32|P-value:4.54E-4||SGD DESC:Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood Gene:BCH2(YKR027W)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication Gene:BEM2(YER155C)|FD-Score:5|P-value:2.94E-7||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence Gene:BRR1(YPR057W)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:snRNP protein component of spliceosomal snRNPs, required for pre-mRNA splicing and snRNP biogenesis; in null mutant newly-synthesized snRNAs are destabilized and 3'-end processing is slowed Gene:BST1(YFL025C)|FD-Score:4.6|P-value:2.14E-6||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CCR4(YAL021C)|FD-Score:8.35|P-value:3.43E-17||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:CCW12(YLR110C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CDC50(YCR094W)|FD-Score:7.79|P-value:3.23E-15||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CIN2(YPL241C)|FD-Score:-3.39|P-value:3.46E-4||SGD DESC:GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2 Gene:CLA4(YNL298W)|FD-Score:-4.42|P-value:4.95E-6||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CSG2(YBR036C)|FD-Score:19.4|P-value:3.31E-84||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:CTM1(YHR109W)|FD-Score:-4.08|P-value:2.22E-5||SGD DESC:Cytochrome c lysine methyltransferase, trimethylates residue 72 of apo-cytochrome c (Cyc1p) in the cytosol; not required for normal respiratory growth Gene:CWH43(YCR017C)|FD-Score:5.94|P-value:1.41E-9||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:DAL81(YIR023W)|FD-Score:4.73|P-value:1.13E-6||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DCG1(YIR030C)|FD-Score:-5.71|P-value:5.56E-9||SGD DESC:Protein of unknown function, expression is sensitive to nitrogen catabolite repression and regulated by Dal80p; contains transmembrane domain Gene:DST1(YGL043W)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress Gene:ECM14(YHR132C)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Putative metalloprotease with similarity to the zinc carboxypeptidase family, required for normal cell wall assembly Gene:ELP6(YMR312W)|FD-Score:-3.38|P-value:3.62E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:GAL2(YLR081W)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Galactose permease, required for utilization of galactose; also able to transport glucose Gene:GAS3(YMR215W)|FD-Score:-3.98|P-value:3.47E-5||SGD DESC:Low abundance, possibly inactive member of the GAS family of GPI-containing proteins; putative 1,3-beta-glucanosyltransferase with similarity to other GAS family members; localizes to the cell wall; mRNA induced during sporulation Gene:GLY1(YEL046C)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GYP1(YOR070C)|FD-Score:6.18|P-value:3.30E-10||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HUA1(YGR268C)|FD-Score:3.92|P-value:4.39E-5||SGD DESC:Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:IAH1(YOR126C)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Isoamyl acetate-hydrolyzing esterase, required in balance with alcohol acetyltransferase to maintain optimal amounts of isoamyl acetate, which is particularly important in sake brewing Gene:ICY2(YPL250C)|FD-Score:4.28|P-value:9.48E-6||SGD DESC:Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate Gene:IKI1(YHR187W)|FD-Score:5.99|P-value:1.06E-9||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin Gene:INP53(YOR109W)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Polyphosphatidylinositol phosphatase, dephosphorylates multiple phosphatidylinositols; involved in trans Golgi network-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches; contains Sac1 and 5-ptase domains Gene:IPT1(YDR072C)|FD-Score:4.55|P-value:2.67E-6||SGD DESC:Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin Gene:IRC14(YOR135C_d)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:IRS4(YKR019C)|FD-Score:8.71|P-value:1.47E-18||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LOA1(YPR139C)|FD-Score:7.35|P-value:1.02E-13||SGD DESC:Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA Gene:LRP1(YHR081W)|FD-Score:-3.4|P-value:3.34E-4||SGD DESC:Nuclear exosome-associated nucleic acid binding protein; involved in RNA processing, surveillance, degradation, tethering, and export; homolog of mammalian nuclear matrix protein C1D involved in regulation of DNA repair and recombination Gene:LRS4(YDR439W)|FD-Score:4|P-value:3.14E-5||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:LSM1(YJL124C)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:LST4(YKL176C)|FD-Score:3.12|P-value:8.94E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MCM21(YDR318W)|FD-Score:3.41|P-value:3.31E-4||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:MDM30(YLR368W)|FD-Score:4.79|P-value:8.43E-7||SGD DESC:F-box component of an SCF ubiquitin protein ligase complex; associates with and is required for Fzo1p ubiquitination and for mitochondria fusion; stimulates nuclear export of specific mRNAs; promotes ubiquitin-mediated degradation of Gal4p in some strains Gene:MDM31(YHR194W)|FD-Score:3.76|P-value:8.34E-5||SGD DESC:Mitochondrial protein that may have a role in phospholipid metabolism; inner membrane protein with similarity to Mdm32p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MMM2, MDM10, MDM12, and MDM34 Gene:MIC23(YBL107C_p)|FD-Score:3.92|P-value:4.37E-5||SGD DESC:Mitochondrial intermembrane space protein of unknown function; imported via the MIA import machinery; contains an unusual twin cysteine motif (CX13C CX14C) Gene:MNN2(YBR015C)|FD-Score:7.85|P-value:2.08E-15||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MNN5(YJL186W)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the second alpha-1,2-linked mannose of the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MUM3(YOR298W)|FD-Score:-3.19|P-value:7.13E-4||SGD DESC:Protein of unknown function involved in the organization of the outer spore wall layers; has similarity to the tafazzins superfamily of acyltransferases Gene:NHX1(YDR456W)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:OPI9(YLR338W_d)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PAM1(YDR251W)|FD-Score:4.52|P-value:3.04E-6||SGD DESC:Essential protein of unknown function; exhibits variable expression during colony morphogenesis; overexpression permits survival without protein phosphatase 2A, inhibits growth, and induces a filamentous phenotype Gene:PDC6(YGR087C)|FD-Score:3.17|P-value:7.67E-4||SGD DESC:Minor isoform of pyruvate decarboxylase, decarboxylates pyruvate to acetaldehyde, involved in amino acid catabolism; transcription is glucose- and ethanol-dependent, and is strongly induced during sulfur limitation Gene:POP2(YNR052C)|FD-Score:5.25|P-value:7.40E-8||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:RGS2(YOR107W)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p Gene:RIF2(YLR453C)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RPL12B(YDR418W)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication Gene:RPL31A(YDL075W)|FD-Score:-6.34|P-value:1.18E-10||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:RPL7B(YPL198W)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Ribosomal 60S subunit protein L7B; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication Gene:RPS14A(YCR031C)|FD-Score:3.09|P-value:9.86E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RPS22A(YJL190C)|FD-Score:3.75|P-value:8.91E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication Gene:RPS7B(YNL096C)|FD-Score:4.79|P-value:8.33E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7B has a paralog, RPS7A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SAC1(YKL212W)|FD-Score:6.62|P-value:1.76E-11||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAC6(YDR129C)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAK1(YER129W)|FD-Score:3.13|P-value:8.70E-4||SGD DESC:Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome Gene:SCS2(YER120W)|FD-Score:-3.24|P-value:5.88E-4||SGD DESC:Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; interacts with FFAT motif of Opi1p; involved in telomeric silencing; null shows inositol auxotrophy above 34 deg C; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication Gene:SLX5(YDL013W)|FD-Score:4.57|P-value:2.43E-6||SGD DESC:Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers Gene:SLX9(YGR081C)|FD-Score:-3.12|P-value:8.95E-4||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SLZ1(YNL196C_p)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Sporulation-specific protein with a leucine zipper motif Gene:SNF7(YLR025W)|FD-Score:4.3|P-value:8.72E-6||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SNF8(YPL002C)|FD-Score:4.23|P-value:1.18E-5||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SPF1(YEL031W)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPL2(YHR136C)|FD-Score:4.49|P-value:3.58E-6||SGD DESC:Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation; overproduction suppresses a plc1 null mutation; GFP-fusion protein localizes to the cytoplasm Gene:SPO7(YAL009W)|FD-Score:5.61|P-value:1.03E-8||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:SSA3(YBL075C)|FD-Score:-3.54|P-value:1.98E-4||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:STP2(YHR006W)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Transcription factor; activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes; STP2 has a paralog, STP1, that arose from the whole genome duplication Gene:SUR1(YPL057C)|FD-Score:8.35|P-value:3.44E-17||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication Gene:SUR4(YLR372W)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWC3(YAL011W)|FD-Score:4.05|P-value:2.61E-5||SGD DESC:Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae Gene:TAE2(YPL009C)|FD-Score:4.71|P-value:1.26E-6||SGD DESC:Protein of unknown function, involved in protein translation; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:TAT1(YBR069C)|FD-Score:3.73|P-value:9.76E-5||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TLG2(YOL018C)|FD-Score:5.36|P-value:4.07E-8||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TPP1(YMR156C)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:TRP2(YER090W)|FD-Score:5.85|P-value:2.52E-9||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:UPS3(YDR185C)|FD-Score:4.42|P-value:5.03E-6||SGD DESC:Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations Gene:VAM10(YOR068C)|FD-Score:3.89|P-value:4.95E-5||SGD DESC:Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering Gene:VBA4(YDR119W_p)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Protein of unknown function with proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene Gene:VIK1(YPL253C)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Protein that forms a complex with Kar3p at the spindle pole body, possible regulator of Kar3p function in microtubule-mediated processes; required for sister chromatid cohesion; has similarity to Cik1p Gene:VMA1(YDL185W)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VPS29(YHR012W)|FD-Score:5.35|P-value:4.33E-8||SGD DESC:Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval Gene:VPS45(YGL095C)|FD-Score:10.1|P-value:2.96E-24||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YBL062W(YBL062W_d)|FD-Score:4.86|P-value:5.86E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL071C(YBL071C_d)|FD-Score:4.01|P-value:2.98E-5||SGD DESC:Dubious open reading frame, predicted protein contains a peroxisomal targeting signal Gene:YCR064C(YCR064C_d)|FD-Score:6.17|P-value:3.45E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YER039C-A(YER039C-A_p)|FD-Score:4.5|P-value:3.36E-6||SGD DESC:Putative protein of unknown function; YER039C-A is not an essential gene Gene:YFL054C(YFL054C_p)|FD-Score:-5.35|P-value:4.52E-8||SGD DESC:Putative channel-like protein; similar to Fps1p; mediates passive diffusion of glycerol in the presence of ethanol Gene:YGL072C(YGL072C_d)|FD-Score:3.91|P-value:4.62E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGL188C-A(YGL188C-A_p)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:Putative protein of unknown function Gene:YHR035W(YHR035W_p)|FD-Score:-3.09|P-value:9.86E-4||SGD DESC:Putative protein of unknown function; not an essential gene Gene:YIL012W(YIL012W_d)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL165C(YIL165C_p)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:Putative protein of unknown function; mutant exhibits mitophagy defects; in closely related species and other S. cerevisiae strain backgrounds YIL165C and adjacent ORF, YIL164C, likely constitute a single ORF encoding a nitrilase gene Gene:YKE4(YIL023C)|FD-Score:-3.23|P-value:6.19E-4||SGD DESC:Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family Gene:YKL018C-A(YKL018C-A_p)|FD-Score:5.37|P-value:3.95E-8||SGD DESC:Putative protein of unknown function; identified by homology; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YLR400W(YLR400W_d)|FD-Score:-3.45|P-value:2.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR434C(YLR434C_d)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W Gene:YMR253C(YMR253C_p)|FD-Score:3.96|P-value:3.72E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene Gene:YNR005C(YNR005C_d)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR131C(YOR131C_p)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:Putative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress Gene:YPR097W(YPR097W)|FD-Score:3.94|P-value:4.09E-5||SGD DESC:Protein that contains a Phox homology (PX) domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YTA7(YGR270W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p Gene:ZAP1(YJL056C)|FD-Score:5.51|P-value:1.81E-8||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains Gene:AFT1(YGL071W)|FD-Score:6.52|P-value:3.50E-11||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:APM4(YOL062C)|FD-Score:-3.24|P-value:5.92E-4||SGD DESC:Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport Gene:ARA1(YBR149W)|FD-Score:6.1|P-value:5.18E-10||SGD DESC:NADP+ dependent arabinose dehydrogenase, involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product Gene:ARC18(YLR370C)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARL1(YBR164C)|FD-Score:3.09|P-value:9.99E-4||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ARP6(YLR085C)|FD-Score:4.06|P-value:2.45E-5||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:ASI1(YMR119W)|FD-Score:3.96|P-value:3.73E-5||SGD DESC:Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication Gene:ATG18(YFR021W)|FD-Score:-3.32|P-value:4.54E-4||SGD DESC:Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood Gene:BCH2(YKR027W)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication Gene:BEM2(YER155C)|FD-Score:5|P-value:2.94E-7||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence Gene:BRR1(YPR057W)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:snRNP protein component of spliceosomal snRNPs, required for pre-mRNA splicing and snRNP biogenesis; in null mutant newly-synthesized snRNAs are destabilized and 3'-end processing is slowed Gene:BST1(YFL025C)|FD-Score:4.6|P-value:2.14E-6||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CCR4(YAL021C)|FD-Score:8.35|P-value:3.43E-17||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:CCW12(YLR110C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CDC50(YCR094W)|FD-Score:7.79|P-value:3.23E-15||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CIN2(YPL241C)|FD-Score:-3.39|P-value:3.46E-4||SGD DESC:GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2 Gene:CLA4(YNL298W)|FD-Score:-4.42|P-value:4.95E-6||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CSG2(YBR036C)|FD-Score:19.4|P-value:3.31E-84||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:CTM1(YHR109W)|FD-Score:-4.08|P-value:2.22E-5||SGD DESC:Cytochrome c lysine methyltransferase, trimethylates residue 72 of apo-cytochrome c (Cyc1p) in the cytosol; not required for normal respiratory growth Gene:CWH43(YCR017C)|FD-Score:5.94|P-value:1.41E-9||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:DAL81(YIR023W)|FD-Score:4.73|P-value:1.13E-6||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DCG1(YIR030C)|FD-Score:-5.71|P-value:5.56E-9||SGD DESC:Protein of unknown function, expression is sensitive to nitrogen catabolite repression and regulated by Dal80p; contains transmembrane domain Gene:DST1(YGL043W)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress Gene:ECM14(YHR132C)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Putative metalloprotease with similarity to the zinc carboxypeptidase family, required for normal cell wall assembly Gene:ELP6(YMR312W)|FD-Score:-3.38|P-value:3.62E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:GAL2(YLR081W)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Galactose permease, required for utilization of galactose; also able to transport glucose Gene:GAS3(YMR215W)|FD-Score:-3.98|P-value:3.47E-5||SGD DESC:Low abundance, possibly inactive member of the GAS family of GPI-containing proteins; putative 1,3-beta-glucanosyltransferase with similarity to other GAS family members; localizes to the cell wall; mRNA induced during sporulation Gene:GLY1(YEL046C)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GYP1(YOR070C)|FD-Score:6.18|P-value:3.30E-10||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HUA1(YGR268C)|FD-Score:3.92|P-value:4.39E-5||SGD DESC:Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:IAH1(YOR126C)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Isoamyl acetate-hydrolyzing esterase, required in balance with alcohol acetyltransferase to maintain optimal amounts of isoamyl acetate, which is particularly important in sake brewing Gene:ICY2(YPL250C)|FD-Score:4.28|P-value:9.48E-6||SGD DESC:Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate Gene:IKI1(YHR187W)|FD-Score:5.99|P-value:1.06E-9||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin Gene:INP53(YOR109W)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Polyphosphatidylinositol phosphatase, dephosphorylates multiple phosphatidylinositols; involved in trans Golgi network-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches; contains Sac1 and 5-ptase domains Gene:IPT1(YDR072C)|FD-Score:4.55|P-value:2.67E-6||SGD DESC:Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin Gene:IRC14(YOR135C_d)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:IRS4(YKR019C)|FD-Score:8.71|P-value:1.47E-18||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LOA1(YPR139C)|FD-Score:7.35|P-value:1.02E-13||SGD DESC:Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA Gene:LRP1(YHR081W)|FD-Score:-3.4|P-value:3.34E-4||SGD DESC:Nuclear exosome-associated nucleic acid binding protein; involved in RNA processing, surveillance, degradation, tethering, and export; homolog of mammalian nuclear matrix protein C1D involved in regulation of DNA repair and recombination Gene:LRS4(YDR439W)|FD-Score:4|P-value:3.14E-5||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:LSM1(YJL124C)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:LST4(YKL176C)|FD-Score:3.12|P-value:8.94E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MCM21(YDR318W)|FD-Score:3.41|P-value:3.31E-4||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:MDM30(YLR368W)|FD-Score:4.79|P-value:8.43E-7||SGD DESC:F-box component of an SCF ubiquitin protein ligase complex; associates with and is required for Fzo1p ubiquitination and for mitochondria fusion; stimulates nuclear export of specific mRNAs; promotes ubiquitin-mediated degradation of Gal4p in some strains Gene:MDM31(YHR194W)|FD-Score:3.76|P-value:8.34E-5||SGD DESC:Mitochondrial protein that may have a role in phospholipid metabolism; inner membrane protein with similarity to Mdm32p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MMM2, MDM10, MDM12, and MDM34 Gene:MIC23(YBL107C_p)|FD-Score:3.92|P-value:4.37E-5||SGD DESC:Mitochondrial intermembrane space protein of unknown function; imported via the MIA import machinery; contains an unusual twin cysteine motif (CX13C CX14C) Gene:MNN2(YBR015C)|FD-Score:7.85|P-value:2.08E-15||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MNN5(YJL186W)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the second alpha-1,2-linked mannose of the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MUM3(YOR298W)|FD-Score:-3.19|P-value:7.13E-4||SGD DESC:Protein of unknown function involved in the organization of the outer spore wall layers; has similarity to the tafazzins superfamily of acyltransferases Gene:NHX1(YDR456W)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:OPI9(YLR338W_d)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PAM1(YDR251W)|FD-Score:4.52|P-value:3.04E-6||SGD DESC:Essential protein of unknown function; exhibits variable expression during colony morphogenesis; overexpression permits survival without protein phosphatase 2A, inhibits growth, and induces a filamentous phenotype Gene:PDC6(YGR087C)|FD-Score:3.17|P-value:7.67E-4||SGD DESC:Minor isoform of pyruvate decarboxylase, decarboxylates pyruvate to acetaldehyde, involved in amino acid catabolism; transcription is glucose- and ethanol-dependent, and is strongly induced during sulfur limitation Gene:POP2(YNR052C)|FD-Score:5.25|P-value:7.40E-8||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:RGS2(YOR107W)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p Gene:RIF2(YLR453C)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RPL12B(YDR418W)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication Gene:RPL31A(YDL075W)|FD-Score:-6.34|P-value:1.18E-10||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:RPL7B(YPL198W)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Ribosomal 60S subunit protein L7B; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication Gene:RPS14A(YCR031C)|FD-Score:3.09|P-value:9.86E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RPS22A(YJL190C)|FD-Score:3.75|P-value:8.91E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication Gene:RPS7B(YNL096C)|FD-Score:4.79|P-value:8.33E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7B has a paralog, RPS7A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SAC1(YKL212W)|FD-Score:6.62|P-value:1.76E-11||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAC6(YDR129C)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAK1(YER129W)|FD-Score:3.13|P-value:8.70E-4||SGD DESC:Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome Gene:SCS2(YER120W)|FD-Score:-3.24|P-value:5.88E-4||SGD DESC:Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; interacts with FFAT motif of Opi1p; involved in telomeric silencing; null shows inositol auxotrophy above 34 deg C; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication Gene:SLX5(YDL013W)|FD-Score:4.57|P-value:2.43E-6||SGD DESC:Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers Gene:SLX9(YGR081C)|FD-Score:-3.12|P-value:8.95E-4||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SLZ1(YNL196C_p)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Sporulation-specific protein with a leucine zipper motif Gene:SNF7(YLR025W)|FD-Score:4.3|P-value:8.72E-6||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SNF8(YPL002C)|FD-Score:4.23|P-value:1.18E-5||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SPF1(YEL031W)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPL2(YHR136C)|FD-Score:4.49|P-value:3.58E-6||SGD DESC:Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation; overproduction suppresses a plc1 null mutation; GFP-fusion protein localizes to the cytoplasm Gene:SPO7(YAL009W)|FD-Score:5.61|P-value:1.03E-8||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:SSA3(YBL075C)|FD-Score:-3.54|P-value:1.98E-4||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:STP2(YHR006W)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Transcription factor; activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes; STP2 has a paralog, STP1, that arose from the whole genome duplication Gene:SUR1(YPL057C)|FD-Score:8.35|P-value:3.44E-17||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication Gene:SUR4(YLR372W)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWC3(YAL011W)|FD-Score:4.05|P-value:2.61E-5||SGD DESC:Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae Gene:TAE2(YPL009C)|FD-Score:4.71|P-value:1.26E-6||SGD DESC:Protein of unknown function, involved in protein translation; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:TAT1(YBR069C)|FD-Score:3.73|P-value:9.76E-5||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TLG2(YOL018C)|FD-Score:5.36|P-value:4.07E-8||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TPP1(YMR156C)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:TRP2(YER090W)|FD-Score:5.85|P-value:2.52E-9||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:UPS3(YDR185C)|FD-Score:4.42|P-value:5.03E-6||SGD DESC:Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations Gene:VAM10(YOR068C)|FD-Score:3.89|P-value:4.95E-5||SGD DESC:Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering Gene:VBA4(YDR119W_p)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Protein of unknown function with proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene Gene:VIK1(YPL253C)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Protein that forms a complex with Kar3p at the spindle pole body, possible regulator of Kar3p function in microtubule-mediated processes; required for sister chromatid cohesion; has similarity to Cik1p Gene:VMA1(YDL185W)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VPS29(YHR012W)|FD-Score:5.35|P-value:4.33E-8||SGD DESC:Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval Gene:VPS45(YGL095C)|FD-Score:10.1|P-value:2.96E-24||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YBL062W(YBL062W_d)|FD-Score:4.86|P-value:5.86E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL071C(YBL071C_d)|FD-Score:4.01|P-value:2.98E-5||SGD DESC:Dubious open reading frame, predicted protein contains a peroxisomal targeting signal Gene:YCR064C(YCR064C_d)|FD-Score:6.17|P-value:3.45E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YER039C-A(YER039C-A_p)|FD-Score:4.5|P-value:3.36E-6||SGD DESC:Putative protein of unknown function; YER039C-A is not an essential gene Gene:YFL054C(YFL054C_p)|FD-Score:-5.35|P-value:4.52E-8||SGD DESC:Putative channel-like protein; similar to Fps1p; mediates passive diffusion of glycerol in the presence of ethanol Gene:YGL072C(YGL072C_d)|FD-Score:3.91|P-value:4.62E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGL188C-A(YGL188C-A_p)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:Putative protein of unknown function Gene:YHR035W(YHR035W_p)|FD-Score:-3.09|P-value:9.86E-4||SGD DESC:Putative protein of unknown function; not an essential gene Gene:YIL012W(YIL012W_d)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL165C(YIL165C_p)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:Putative protein of unknown function; mutant exhibits mitophagy defects; in closely related species and other S. cerevisiae strain backgrounds YIL165C and adjacent ORF, YIL164C, likely constitute a single ORF encoding a nitrilase gene Gene:YKE4(YIL023C)|FD-Score:-3.23|P-value:6.19E-4||SGD DESC:Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family Gene:YKL018C-A(YKL018C-A_p)|FD-Score:5.37|P-value:3.95E-8||SGD DESC:Putative protein of unknown function; identified by homology; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YLR400W(YLR400W_d)|FD-Score:-3.45|P-value:2.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR434C(YLR434C_d)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W Gene:YMR253C(YMR253C_p)|FD-Score:3.96|P-value:3.72E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene Gene:YNR005C(YNR005C_d)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR131C(YOR131C_p)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:Putative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress Gene:YPR097W(YPR097W)|FD-Score:3.94|P-value:4.09E-5||SGD DESC:Protein that contains a Phox homology (PX) domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YTA7(YGR270W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p Gene:ZAP1(YJL056C)|FD-Score:5.51|P-value:1.81E-8||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YFL039C6.271.76E-101.35ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YHL015W5.852.45E-91.35RPS20Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10
YPL169C5.764.11E-91.35MEX67Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP
YAR007C4.415.19E-60.60RFA1Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination
YLR275W3.816.98E-50.04SMD2Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2
YNL272C3.778.29E-50.05SEC2Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles
YDR353W3.721.02E-40.11TRR1Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication
YBR211C3.601.57E-48.00E-4AME1Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress
YMR033W3.601.58E-40.04ARP9Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YPR133C3.561.82E-40.14SPN1Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype
YMR043W3.433.07E-40.05MCM1Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes
YKL058W3.373.69E-40.01TOA2TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress
YHR069C3.373.79E-40.10RRP4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2)
YLR116W3.275.44E-40.00MSL5Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA
YOR046C3.265.52E-40.07DBP5Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus; involved in translation termination; ATP/ADP cycling is regulated by Gle1p and Nup159p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR036C19.403.31E-84CSG2Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress
YGL095C10.102.96E-24VPS45Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment
YKR019C8.711.47E-18IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YAL021C8.353.43E-17CCR4Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening
YPL057C8.353.44E-17SUR1Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication
YBR015C7.852.08E-15MNN2Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment
YCR094W7.793.23E-15CDC50Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication
YPR139C7.351.02E-13LOA1Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA
YKL212W6.621.76E-11SAC1Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism
YGL071W6.523.50E-11AFT1Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YOR070C6.183.30E-10GYP1Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion
YCR064C_d6.173.45E-10YCR064C_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31
YBR149W6.105.18E-10ARA1NADP+ dependent arabinose dehydrogenase, involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product
YHR187W5.991.06E-9IKI1Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin
YCR017C5.941.41E-9CWH43Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion

GO enrichment analysis for SGTC_2873
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2162.82E-63SGTC_232clozapine 119.0 μMMiscellaneous28180.0853659NEO1
0.1575.37E-34SGTC_28679039098 71.4 μMChembridge (Drug-like library)64634600.141026iron homeostasis
0.1541.11E-32SGTC_3014119-0100 24.5 μMChemDiv (Drug-like library)28010.0886076NEO1
0.1542.39E-32SGTC_439amiodarone 34.4 μMMiscellaneous21570.120879NEO1-PIK1
0.1533.70E-32SGTC_470aa-861 153.0 μMICCB bioactive library19670.0684932sphingolipid biosynthesis & PDR1
0.1483.46E-30SGTC_2064546-0025 72.0 μMChemDiv (Drug-like library)66207450.0813954NEO1
0.1479.95E-30SGTC_6051315-0399 119.0 μMChemDiv (Drug-like library)240204610.0795455Golgi
0.1462.18E-29SGTC_21685727979 200.0 μMChembridge (Fragment library)6952100.115942
0.1382.91E-26SGTC_221trifluoperazine 10.3 μMMiscellaneous55660.0941176NEO1-PIK1
0.1322.06E-24SGTC_2658polymyxin b sulfate 77.0 μMMicrosource (Natural product library)57021050.0952381NEO1
0.1262.46E-22SGTC_29349036370 36.9 μMChembridge (Drug-like library)64628000.777778
0.1241.53E-21SGTC_31529099253 49.5 μMChembridge (Drug-like library)252361310.15NEO1-PIK1
0.1231.72E-21SGTC_24725377652 49.4 μMMiscellaneous16109030.106667TRP & mitochondrial translation
0.1231.98E-21SGTC_9551216-0110 33.0 μMChemDiv (Drug-like library)36197190.116883sphingolipid biosynthesis & PDR1
0.1224.28E-21SGTC_13943474-0001 65.7 μMChemDiv (Drug-like library)50176660.0875endomembrane recycling

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2934903637036.88 μM0.7777786462800Chembridge (Drug-like library)348.810623.01316
SGTC_2954907481852.69 μM0.66071416648742Chembridge (Drug-like library)342.418723.29116
SGTC_3201911399949.47 μM0.44444416649224Chembridge (Drug-like library)336.38773.90314
SGTC_3355915105844.05 μM0.417015728Chembridge (Drug-like library)311.3136632.87125RNA processing & uracil transport
SGTC_3290912103171.43 μM0.39130417014854Chembridge (Drug-like library)354.3781634.00915
SGTC_3270913756349.47 μM0.38095217015724Chembridge (Drug-like library)293.32322.66624RPP1 & pyrimidine depletion
SGTC_3079911751249.47 μM0.3731347185044Chembridge (Drug-like library)338.360522.91515
SGTC_3168910341949.47 μM0.37313416649256Chembridge (Drug-like library)308.334542.9311460S ribosome export
SGTC_3245913482149.47 μM0.36363617014901Chembridge (Drug-like library)307.349783.12224
SGTC_3065911388849.47 μM0.35294116648368Chembridge (Drug-like library)338.360522.91515