9044784

2-[4-[(4-methylphenyl)methyl]piperazin-1-yl]-N-phenylacetamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2887
Screen concentration 58.4 μM
Source Chembridge (Drug-like library)
PubChem CID 6465546
SMILES CC1=CC=C(C=C1)CN2CCN(CC2)CC(=O)NC3=CC=CC=C3
Standardized SMILES Cc1ccc(CN2CCN(CC(=O)Nc3ccccc3)CC2)cc1
Molecular weight 323.432
ALogP 2.98
H-bond donor count 1
H-bond acceptor count 3
Response signature fatty acid desaturase (OLE1)

Pool Growth Kinetics
% growth inhibition (Het. pool) 16.5
% growth inhibition (Hom. pool) 7.41


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6465546
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:-3.41|P-value:3.23E-4|Clearance:0||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:CCT2(YIL142W)|FD-Score:3.39|P-value:3.55E-4|Clearance:0||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT5(YJR064W)|FD-Score:3.38|P-value:3.67E-4|Clearance:0.04||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC25(YLR310C)|FD-Score:-4.31|P-value:8.26E-6|Clearance:0||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:DOP1(YDR141C)|FD-Score:4.35|P-value:6.73E-6|Clearance:0||SGD DESC:Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies Gene:ERB1(YMR049C)|FD-Score:3.34|P-value:4.26E-4|Clearance:0.05||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1 Gene:GCD11(YER025W)|FD-Score:3.41|P-value:3.27E-4|Clearance:0.01||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:GLC7(YER133W)|FD-Score:-3.33|P-value:4.27E-4|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HEM12(YDR047W)|FD-Score:7|P-value:1.31E-12|Clearance:0.73||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HSH49(YOR319W)|FD-Score:4.9|P-value:4.73E-7|Clearance:0.4||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:HSP10(YOR020C)|FD-Score:-5.83|P-value:2.77E-9|Clearance:0||SGD DESC:Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES Gene:LAS17(YOR181W)|FD-Score:3.24|P-value:5.90E-4|Clearance:0.24||SGD DESC:Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP) Gene:MTR2(YKL186C)|FD-Score:3.28|P-value:5.12E-4|Clearance:0.03||SGD DESC:mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA Gene:NIP1(YMR309C)|FD-Score:3.25|P-value:5.69E-4|Clearance:0.01||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:NMD3(YHR170W)|FD-Score:4.06|P-value:2.46E-5|Clearance:0||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOC2(YOR206W)|FD-Score:5.64|P-value:8.73E-9|Clearance:0.26||SGD DESC:Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors Gene:NOG1(YPL093W)|FD-Score:3.82|P-value:6.73E-5|Clearance:0.01||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:NOG2(YNR053C)|FD-Score:7.75|P-value:4.72E-15|Clearance:0.61||SGD DESC:Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation Gene:NOP8(YOL144W)|FD-Score:5.11|P-value:1.61E-7|Clearance:0.21||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:NSP1(YJL041W)|FD-Score:-3.17|P-value:7.70E-4|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p); forms foci at the nuclear periphery upon DNA replication stress Gene:OLE1(YGL055W)|FD-Score:9.44|P-value:1.82E-21|Clearance:0.82||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PKC1(YBL105C)|FD-Score:4.38|P-value:5.84E-6|Clearance:0.03||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RLP24(YLR009W)|FD-Score:8.87|P-value:3.71E-19|Clearance:0.82||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPA190(YOR341W)|FD-Score:-4.08|P-value:2.25E-5|Clearance:0||SGD DESC:RNA polymerase I largest subunit A190 Gene:RPL18A(YOL120C)|FD-Score:3.83|P-value:6.52E-5|Clearance:0.01||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPL30(YGL030W)|FD-Score:5.23|P-value:8.49E-8|Clearance:0.12||SGD DESC:Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog Gene:RPN6(YDL097C)|FD-Score:4.06|P-value:2.46E-5|Clearance:0.23||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:RPS5(YJR123W)|FD-Score:4.48|P-value:3.68E-6|Clearance:0.1||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:RRP43(YCR035C)|FD-Score:3.38|P-value:3.58E-4|Clearance:0.01||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress Gene:SEC27(YGL137W)|FD-Score:-3.15|P-value:8.29E-4|Clearance:0||SGD DESC:Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP Gene:SUI2(YJR007W)|FD-Score:6.27|P-value:1.85E-10|Clearance:0.63||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TIM17(YJL143W)|FD-Score:3.59|P-value:1.65E-4|Clearance:0.12||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:TOM22(YNL131W)|FD-Score:3.81|P-value:6.89E-5|Clearance:0.17||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:TOM40(YMR203W)|FD-Score:8.05|P-value:4.30E-16|Clearance:0.3||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TRR1(YDR353W)|FD-Score:3.47|P-value:2.58E-4|Clearance:0.06||SGD DESC:Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication Gene:UTP8(YGR128C)|FD-Score:4.1|P-value:2.05E-5|Clearance:0.04||SGD DESC:Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YBR190W(YBR190W_d)|FD-Score:7.14|P-value:4.74E-13|Clearance:0.14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YDL196W(YDL196W_d)|FD-Score:5.38|P-value:3.82E-8|Clearance:0.15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YJL009W(YJL009W_d)|FD-Score:3.64|P-value:1.37E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex Gene:YJL086C(YJL086C_d)|FD-Score:3.4|P-value:3.36E-4|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YLR076C(YLR076C_d)|FD-Score:4.35|P-value:6.84E-6|Clearance:0.25||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:YMR290W-A(YMR290W-A_d)|FD-Score:4.5|P-value:3.44E-6|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:ACT1(YFL039C)|FD-Score:-3.41|P-value:3.23E-4|Clearance:0||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:CCT2(YIL142W)|FD-Score:3.39|P-value:3.55E-4|Clearance:0||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT5(YJR064W)|FD-Score:3.38|P-value:3.67E-4|Clearance:0.04||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC25(YLR310C)|FD-Score:-4.31|P-value:8.26E-6|Clearance:0||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:DOP1(YDR141C)|FD-Score:4.35|P-value:6.73E-6|Clearance:0||SGD DESC:Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies Gene:ERB1(YMR049C)|FD-Score:3.34|P-value:4.26E-4|Clearance:0.05||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1 Gene:GCD11(YER025W)|FD-Score:3.41|P-value:3.27E-4|Clearance:0.01||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:GLC7(YER133W)|FD-Score:-3.33|P-value:4.27E-4|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HEM12(YDR047W)|FD-Score:7|P-value:1.31E-12|Clearance:0.73||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HSH49(YOR319W)|FD-Score:4.9|P-value:4.73E-7|Clearance:0.4||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:HSP10(YOR020C)|FD-Score:-5.83|P-value:2.77E-9|Clearance:0||SGD DESC:Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES Gene:LAS17(YOR181W)|FD-Score:3.24|P-value:5.90E-4|Clearance:0.24||SGD DESC:Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP) Gene:MTR2(YKL186C)|FD-Score:3.28|P-value:5.12E-4|Clearance:0.03||SGD DESC:mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA Gene:NIP1(YMR309C)|FD-Score:3.25|P-value:5.69E-4|Clearance:0.01||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:NMD3(YHR170W)|FD-Score:4.06|P-value:2.46E-5|Clearance:0||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOC2(YOR206W)|FD-Score:5.64|P-value:8.73E-9|Clearance:0.26||SGD DESC:Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors Gene:NOG1(YPL093W)|FD-Score:3.82|P-value:6.73E-5|Clearance:0.01||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:NOG2(YNR053C)|FD-Score:7.75|P-value:4.72E-15|Clearance:0.61||SGD DESC:Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation Gene:NOP8(YOL144W)|FD-Score:5.11|P-value:1.61E-7|Clearance:0.21||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:NSP1(YJL041W)|FD-Score:-3.17|P-value:7.70E-4|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p); forms foci at the nuclear periphery upon DNA replication stress Gene:OLE1(YGL055W)|FD-Score:9.44|P-value:1.82E-21|Clearance:0.82||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PKC1(YBL105C)|FD-Score:4.38|P-value:5.84E-6|Clearance:0.03||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RLP24(YLR009W)|FD-Score:8.87|P-value:3.71E-19|Clearance:0.82||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPA190(YOR341W)|FD-Score:-4.08|P-value:2.25E-5|Clearance:0||SGD DESC:RNA polymerase I largest subunit A190 Gene:RPL18A(YOL120C)|FD-Score:3.83|P-value:6.52E-5|Clearance:0.01||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPL30(YGL030W)|FD-Score:5.23|P-value:8.49E-8|Clearance:0.12||SGD DESC:Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog Gene:RPN6(YDL097C)|FD-Score:4.06|P-value:2.46E-5|Clearance:0.23||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:RPS5(YJR123W)|FD-Score:4.48|P-value:3.68E-6|Clearance:0.1||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:RRP43(YCR035C)|FD-Score:3.38|P-value:3.58E-4|Clearance:0.01||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress Gene:SEC27(YGL137W)|FD-Score:-3.15|P-value:8.29E-4|Clearance:0||SGD DESC:Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP Gene:SUI2(YJR007W)|FD-Score:6.27|P-value:1.85E-10|Clearance:0.63||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TIM17(YJL143W)|FD-Score:3.59|P-value:1.65E-4|Clearance:0.12||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:TOM22(YNL131W)|FD-Score:3.81|P-value:6.89E-5|Clearance:0.17||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:TOM40(YMR203W)|FD-Score:8.05|P-value:4.30E-16|Clearance:0.3||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TRR1(YDR353W)|FD-Score:3.47|P-value:2.58E-4|Clearance:0.06||SGD DESC:Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication Gene:UTP8(YGR128C)|FD-Score:4.1|P-value:2.05E-5|Clearance:0.04||SGD DESC:Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YBR190W(YBR190W_d)|FD-Score:7.14|P-value:4.74E-13|Clearance:0.14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YDL196W(YDL196W_d)|FD-Score:5.38|P-value:3.82E-8|Clearance:0.15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YJL009W(YJL009W_d)|FD-Score:3.64|P-value:1.37E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex Gene:YJL086C(YJL086C_d)|FD-Score:3.4|P-value:3.36E-4|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YLR076C(YLR076C_d)|FD-Score:4.35|P-value:6.84E-6|Clearance:0.25||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:YMR290W-A(YMR290W-A_d)|FD-Score:4.5|P-value:3.44E-6|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6465546
Download HOP data (tab-delimited text)  (excel)
Gene:ADO1(YJR105W)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AEP2(YMR282C)|FD-Score:-3.6|P-value:1.56E-4||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AKR1(YDR264C)|FD-Score:4.15|P-value:1.68E-5||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:AMN1(YBR158W)|FD-Score:3.74|P-value:9.14E-5||SGD DESC:Protein required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN) Gene:APN1(YKL114C)|FD-Score:-3.4|P-value:3.31E-4||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:APP1(YNL094W)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway Gene:ARL1(YBR164C)|FD-Score:-3.62|P-value:1.47E-4||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ARO2(YGL148W)|FD-Score:9.43|P-value:1.97E-21||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ATG16(YMR159C)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which localize to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:ATG17(YLR423C)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells Gene:ATG7(YHR171W)|FD-Score:4.49|P-value:3.49E-6||SGD DESC:Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation Gene:ATP7(YKL016C)|FD-Score:-3.21|P-value:6.59E-4||SGD DESC:Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BCH1(YMR237W)|FD-Score:4.06|P-value:2.47E-5||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication Gene:BIO2(YGR286C)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant Gene:BUD14(YAR014C)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress Gene:BUD23(YCR047C)|FD-Score:-5.07|P-value:2.01E-7||SGD DESC:Methyltransferase, methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern Gene:CAF20(YOR276W)|FD-Score:-4.22|P-value:1.24E-5||SGD DESC:Phosphoprotein of the mRNA cap-binding complex involved in translational control, repressor of cap-dependent translation initiation, competes with eIF4G for binding to eIF4E Gene:CBF1(YJR060W)|FD-Score:5.42|P-value:2.99E-8||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:CDC50(YCR094W)|FD-Score:13.3|P-value:1.66E-40||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CLC1(YGR167W)|FD-Score:-3.12|P-value:9.04E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COG5(YNL051W)|FD-Score:-3.78|P-value:7.93E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG6(YNL041C)|FD-Score:-3.83|P-value:6.42E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:-3.73|P-value:9.74E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:-3.33|P-value:4.33E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COS10(YNR075W)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:DAN1(YJR150C)|FD-Score:-3.64|P-value:1.39E-4||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:DID4(YKL002W)|FD-Score:4.43|P-value:4.69E-6||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:DIE2(YGR227W)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1 Gene:DRS2(YAL026C)|FD-Score:4.76|P-value:9.48E-7||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:ECM14(YHR132C)|FD-Score:6.38|P-value:8.60E-11||SGD DESC:Putative metalloprotease with similarity to the zinc carboxypeptidase family, required for normal cell wall assembly Gene:ECM38(YLR299W)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Gamma-glutamyltranspeptidase, major glutathione-degrading enzyme; involved in detoxification of electrophilic xenobiotics; expression induced mainly by nitrogen starvation Gene:GCS1(YDL226C)|FD-Score:25.4|P-value:4.49E-143||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GDB1(YPR184W)|FD-Score:3.18|P-value:7.25E-4||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GET3(YDL100C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:IRC20(YLR247C)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Putative helicase; localizes to the mitochondrion and the nucleus; YLR247C is not an essential gene; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRS4(YKR019C)|FD-Score:-5.1|P-value:1.66E-7||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:JNM1(YMR294W)|FD-Score:3.15|P-value:8.11E-4||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:KAR9(YPL269W)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase Gene:KIN82(YCR091W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Putative serine/threonine protein kinase implicated in the regulation of phospholipid asymmetry through the activation of phospholipid translocases (flippases) Lem3p-Dnf1p/Dnf2p; similar to Fpk1p Gene:KTR7(YIL085C)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication Gene:LDB18(YLL049W)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Component of the dynactin complex, which is required for dynein activity; null mutant exhibits defects in nuclear migration and spindle orientation and has reduced affinity for alcian blue dye; has homology to mammalian dynactin subunit p24 Gene:LOA1(YPR139C)|FD-Score:8.98|P-value:1.37E-19||SGD DESC:Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA Gene:LRE1(YCL051W)|FD-Score:-3.44|P-value:2.87E-4||SGD DESC:Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway; <br>LRE1 has a paralog, HLR1, that arose from the whole genome duplication Gene:MCT1(YOR221C)|FD-Score:-3.29|P-value:5.07E-4||SGD DESC:Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling Gene:MCX1(YBR227C)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Mitochondrial matrix protein; putative ATP-binding chaperone with non-proteolytic function; similar to bacterial ClpX proteins Gene:MDM35(YKL053C-A)|FD-Score:3.79|P-value:7.57E-5||SGD DESC:Mitochondrial intermembrane space protein; mutation affects mitochondrial distribution and morphology; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MEF2(YJL102W)|FD-Score:-3.38|P-value:3.68E-4||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MGM1(YOR211C)|FD-Score:-3.51|P-value:2.28E-4||SGD DESC:Mitochondrial GTPase, present in complex with Ugo1p and Fzo1p; required for mt morphology and genome maintenance; exists as long and short form with different distributions; homolog of human OPA1 involved in autosomal dominant optic atrophy Gene:MON1(YGL124C)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate Gene:MRC1(YCL061C)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres Gene:MRPL51(YPR100W)|FD-Score:-4.53|P-value:2.89E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSA2(YKR077W)|FD-Score:4.77|P-value:9.43E-7||SGD DESC:Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation Gene:MSI1(YBR195C)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription; Msi1p localizes to both nucleus and cytoplasm and has an independent role as a negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase Gene:MSR1(YHR091C)|FD-Score:-3.25|P-value:5.69E-4||SGD DESC:Mitochondrial arginyl-tRNA synthetase; mutations in human ortholog are associated with pontocerebellar hypoplasia type 6 Gene:MSW1(YDR268W)|FD-Score:-3.13|P-value:8.86E-4||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:NHP10(YDL002C)|FD-Score:3.83|P-value:6.37E-5||SGD DESC:Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair Gene:NHP6A(YPR052C)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:NUP133(YKR082W)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP133 Gene:OCA6(YDR067C_p)|FD-Score:4.39|P-value:5.57E-6||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT Gene:OM14(YBR230C)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron Gene:OPI3(YJR073C)|FD-Score:-5.08|P-value:1.90E-7||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:ORM2(YLR350W)|FD-Score:3.17|P-value:7.74E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress Gene:OSW1(YOR255W)|FD-Score:-4.24|P-value:1.14E-5||SGD DESC:Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p Gene:PAP2(YOL115W)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Trf5p Gene:PAU4(YLR461W)|FD-Score:3.1|P-value:9.69E-4||SGD DESC:Member of the seripauperin multigene family encoded mainly in subtelomeric regions; active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme Gene:PDC5(YLR134W)|FD-Score:3.94|P-value:4.06E-5||SGD DESC:Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism Gene:PEP7(YDR323C)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PEX28(YHR150W)|FD-Score:4.89|P-value:4.92E-7||SGD DESC:Peroxisomal integral membrane peroxin, involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p Gene:PEX32(YBR168W)|FD-Score:-3.12|P-value:9.20E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PHO87(YCR037C)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication Gene:PUS4(YNL292W)|FD-Score:4.53|P-value:3.01E-6||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype) Gene:RCY1(YJL204C)|FD-Score:14.1|P-value:1.59E-45||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:REI1(YBR267W)|FD-Score:3.17|P-value:7.73E-4||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RGP1(YDR137W)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIC1(YLR039C)|FD-Score:-3.93|P-value:4.26E-5||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPL23B(YER117W)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Ribosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication Gene:RPL2A(YFR031C-A)|FD-Score:-3.72|P-value:9.90E-5||SGD DESC:Ribosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication Gene:RPL6B(YLR448W)|FD-Score:-4.18|P-value:1.44E-5||SGD DESC:Ribosomal 60S subunit protein L6B; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6B has a paralog, RPL6A, that arose from the whole genome duplication Gene:RRG9(YNL213C)|FD-Score:-4.35|P-value:6.82E-6||SGD DESC:Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RTS3(YGR161C_p)|FD-Score:3.95|P-value:3.84E-5||SGD DESC:Putative component of the protein phosphatase type 2A complex Gene:SLG1(YOR008C)|FD-Score:-3.22|P-value:6.34E-4||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SNT309(YPR101W)|FD-Score:-3.8|P-value:7.32E-5||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SOL2(YCR073W-A)|FD-Score:4.75|P-value:1.01E-6||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:SPH1(YLR313C)|FD-Score:4.32|P-value:7.78E-6||SGD DESC:Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p Gene:SPT3(YDR392W)|FD-Score:-5.31|P-value:5.63E-8||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:STP3(YLR375W)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Zinc-finger protein of unknown function, possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids Gene:SWI3(YJL176C)|FD-Score:-3.43|P-value:3.05E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions Gene:TRP1(YDR007W)|FD-Score:8.76|P-value:1.00E-18||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP3(YKL211C)|FD-Score:4.52|P-value:3.12E-6||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:ULS1(YOR191W)|FD-Score:5.38|P-value:3.66E-8||SGD DESC:Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:UPC2(YDR213W)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Sterol regulatory element binding protein; induces transcription of sterol biosynthetic genes and of DAN/TIR gene products; relocates from intracellular membranes to perinuclear foci on sterol depletion; UPC2 has a paralog, ECM22, that arose from the whole genome duplication Gene:USA1(YML029W)|FD-Score:3.29|P-value:5.00E-4||SGD DESC:Scaffold subunit of the Hrd1p ubiquitin ligase that also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p Gene:VMA22(YHR060W)|FD-Score:-4.22|P-value:1.21E-5||SGD DESC:Peripheral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) Gene:VPS3(YDR495C)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS61(YDR136C_d)|FD-Score:-4.35|P-value:6.90E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YAL065C(YAL065C_p)|FD-Score:4.89|P-value:5.07E-7||SGD DESC:Putative protein of unknown function; has homology to FLO1; possible pseudogene Gene:YBR232C(YBR232C_d)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCL022C(YCL022C_d)|FD-Score:-3.12|P-value:8.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene KCC4/YCL024W Gene:YDC1(YPL087W)|FD-Score:3.84|P-value:6.22E-5||SGD DESC:Alkaline dihydroceramidase, involved in sphingolipid metabolism; preferentially hydrolyzes dihydroceramide to a free fatty acid and dihydrosphingosine; has a minor reverse activity; YDC1 has a paralog, YPC1, that arose from the whole genome duplication Gene:YDL187C(YDL187C_d)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR161W(YDR161W_p)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YER119C-A(YER119C-A_d)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YFR039C(YFR039C_p)|FD-Score:-4.34|P-value:7.01E-6||SGD DESC:Putative protein of unknown function; may be involved in response to high salt and changes in carbon source; deletion mutant has decreased spore survival in Drosophila feces Gene:YGL072C(YGL072C_d)|FD-Score:5.08|P-value:1.89E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGR111W(YGR111W_p)|FD-Score:3.2|P-value:6.86E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YGR237C(YGR237C_p)|FD-Score:-3.23|P-value:6.18E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YHR033W(YHR033W_p)|FD-Score:-3.74|P-value:9.28E-5||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YHR033W has a paralog, PRO1, that arose from the whole genome duplication Gene:YIR024C(YIR024C)|FD-Score:4.49|P-value:3.61E-6||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; interacts with Arh1p, a mitochondrial oxidoreductase; deletion mutant has a respiratory growth defect Gene:YKE4(YIL023C)|FD-Score:-3.51|P-value:2.28E-4||SGD DESC:Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family Gene:YLL044W(YLL044W_d)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLL044W and the overlapping gene RPL8B is reduced in the gcr1 null mutant Gene:YLL047W(YLL047W_d)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RNP1 Gene:YLR211C(YLR211C_p)|FD-Score:-3.65|P-value:1.33E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR211C is not an essential gene; ORF contains an intron Gene:YLR225C(YLR225C_p)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene Gene:YML007C-A(YML007C-A_p)|FD-Score:3.78|P-value:7.83E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YML018C(YML018C_p)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication Gene:YML122C(YML122C_d)|FD-Score:-3.88|P-value:5.20E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR027W(YMR027W_p)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR027W is not an essential gene Gene:YMR242W-A(YMR242W-A_p)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Putative protein of unknown function Gene:YNL184C(YNL184C_p)|FD-Score:-4.44|P-value:4.42E-6||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOL046C(YOL046C_d)|FD-Score:-3.45|P-value:2.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data;almost completely overlaps the verified gene PSK2/YOL045W Gene:YOR041C(YOR041C_d)|FD-Score:-4.88|P-value:5.24E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the uncharacterized gene CUE5; deletion in cyr1 mutant results in loss of stress resistance Gene:YOR082C(YOR082C_d)|FD-Score:-3.61|P-value:1.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene YOR083W Gene:YOR366W(YOR366W_d)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C Gene:YOR385W(YOR385W_p)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:YPK1(YKL126W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL182C(YPL182C_d)|FD-Score:-3.6|P-value:1.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W Gene:YPL260W(YPL260W_p)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Putative substrate of cAMP-dependent protein kinase (PKA); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YPL260W is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YPR077C(YPR077C_d)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1 Gene:YPS6(YIR039C)|FD-Score:3.94|P-value:4.09E-5||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance Gene:YPT31(YER031C)|FD-Score:3.77|P-value:8.30E-5||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:YPT6(YLR262C)|FD-Score:-4.4|P-value:5.53E-6||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YSC84(YHR016C)|FD-Score:3.19|P-value:7.12E-4||SGD DESC:Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication Gene:ADO1(YJR105W)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AEP2(YMR282C)|FD-Score:-3.6|P-value:1.56E-4||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AKR1(YDR264C)|FD-Score:4.15|P-value:1.68E-5||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:AMN1(YBR158W)|FD-Score:3.74|P-value:9.14E-5||SGD DESC:Protein required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN) Gene:APN1(YKL114C)|FD-Score:-3.4|P-value:3.31E-4||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:APP1(YNL094W)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway Gene:ARL1(YBR164C)|FD-Score:-3.62|P-value:1.47E-4||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ARO2(YGL148W)|FD-Score:9.43|P-value:1.97E-21||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ATG16(YMR159C)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which localize to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:ATG17(YLR423C)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells Gene:ATG7(YHR171W)|FD-Score:4.49|P-value:3.49E-6||SGD DESC:Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation Gene:ATP7(YKL016C)|FD-Score:-3.21|P-value:6.59E-4||SGD DESC:Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BCH1(YMR237W)|FD-Score:4.06|P-value:2.47E-5||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication Gene:BIO2(YGR286C)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant Gene:BUD14(YAR014C)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress Gene:BUD23(YCR047C)|FD-Score:-5.07|P-value:2.01E-7||SGD DESC:Methyltransferase, methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern Gene:CAF20(YOR276W)|FD-Score:-4.22|P-value:1.24E-5||SGD DESC:Phosphoprotein of the mRNA cap-binding complex involved in translational control, repressor of cap-dependent translation initiation, competes with eIF4G for binding to eIF4E Gene:CBF1(YJR060W)|FD-Score:5.42|P-value:2.99E-8||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:CDC50(YCR094W)|FD-Score:13.3|P-value:1.66E-40||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CLC1(YGR167W)|FD-Score:-3.12|P-value:9.04E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COG5(YNL051W)|FD-Score:-3.78|P-value:7.93E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG6(YNL041C)|FD-Score:-3.83|P-value:6.42E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:-3.73|P-value:9.74E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:-3.33|P-value:4.33E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COS10(YNR075W)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:DAN1(YJR150C)|FD-Score:-3.64|P-value:1.39E-4||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:DID4(YKL002W)|FD-Score:4.43|P-value:4.69E-6||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:DIE2(YGR227W)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1 Gene:DRS2(YAL026C)|FD-Score:4.76|P-value:9.48E-7||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:ECM14(YHR132C)|FD-Score:6.38|P-value:8.60E-11||SGD DESC:Putative metalloprotease with similarity to the zinc carboxypeptidase family, required for normal cell wall assembly Gene:ECM38(YLR299W)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Gamma-glutamyltranspeptidase, major glutathione-degrading enzyme; involved in detoxification of electrophilic xenobiotics; expression induced mainly by nitrogen starvation Gene:GCS1(YDL226C)|FD-Score:25.4|P-value:4.49E-143||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GDB1(YPR184W)|FD-Score:3.18|P-value:7.25E-4||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GET3(YDL100C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:IRC20(YLR247C)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Putative helicase; localizes to the mitochondrion and the nucleus; YLR247C is not an essential gene; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRS4(YKR019C)|FD-Score:-5.1|P-value:1.66E-7||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:JNM1(YMR294W)|FD-Score:3.15|P-value:8.11E-4||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:KAR9(YPL269W)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase Gene:KIN82(YCR091W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Putative serine/threonine protein kinase implicated in the regulation of phospholipid asymmetry through the activation of phospholipid translocases (flippases) Lem3p-Dnf1p/Dnf2p; similar to Fpk1p Gene:KTR7(YIL085C)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication Gene:LDB18(YLL049W)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Component of the dynactin complex, which is required for dynein activity; null mutant exhibits defects in nuclear migration and spindle orientation and has reduced affinity for alcian blue dye; has homology to mammalian dynactin subunit p24 Gene:LOA1(YPR139C)|FD-Score:8.98|P-value:1.37E-19||SGD DESC:Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA Gene:LRE1(YCL051W)|FD-Score:-3.44|P-value:2.87E-4||SGD DESC:Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway; <br>LRE1 has a paralog, HLR1, that arose from the whole genome duplication Gene:MCT1(YOR221C)|FD-Score:-3.29|P-value:5.07E-4||SGD DESC:Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling Gene:MCX1(YBR227C)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Mitochondrial matrix protein; putative ATP-binding chaperone with non-proteolytic function; similar to bacterial ClpX proteins Gene:MDM35(YKL053C-A)|FD-Score:3.79|P-value:7.57E-5||SGD DESC:Mitochondrial intermembrane space protein; mutation affects mitochondrial distribution and morphology; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MEF2(YJL102W)|FD-Score:-3.38|P-value:3.68E-4||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MGM1(YOR211C)|FD-Score:-3.51|P-value:2.28E-4||SGD DESC:Mitochondrial GTPase, present in complex with Ugo1p and Fzo1p; required for mt morphology and genome maintenance; exists as long and short form with different distributions; homolog of human OPA1 involved in autosomal dominant optic atrophy Gene:MON1(YGL124C)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate Gene:MRC1(YCL061C)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres Gene:MRPL51(YPR100W)|FD-Score:-4.53|P-value:2.89E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSA2(YKR077W)|FD-Score:4.77|P-value:9.43E-7||SGD DESC:Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation Gene:MSI1(YBR195C)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription; Msi1p localizes to both nucleus and cytoplasm and has an independent role as a negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase Gene:MSR1(YHR091C)|FD-Score:-3.25|P-value:5.69E-4||SGD DESC:Mitochondrial arginyl-tRNA synthetase; mutations in human ortholog are associated with pontocerebellar hypoplasia type 6 Gene:MSW1(YDR268W)|FD-Score:-3.13|P-value:8.86E-4||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:NHP10(YDL002C)|FD-Score:3.83|P-value:6.37E-5||SGD DESC:Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair Gene:NHP6A(YPR052C)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:NUP133(YKR082W)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP133 Gene:OCA6(YDR067C_p)|FD-Score:4.39|P-value:5.57E-6||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT Gene:OM14(YBR230C)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron Gene:OPI3(YJR073C)|FD-Score:-5.08|P-value:1.90E-7||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:ORM2(YLR350W)|FD-Score:3.17|P-value:7.74E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress Gene:OSW1(YOR255W)|FD-Score:-4.24|P-value:1.14E-5||SGD DESC:Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p Gene:PAP2(YOL115W)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Trf5p Gene:PAU4(YLR461W)|FD-Score:3.1|P-value:9.69E-4||SGD DESC:Member of the seripauperin multigene family encoded mainly in subtelomeric regions; active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme Gene:PDC5(YLR134W)|FD-Score:3.94|P-value:4.06E-5||SGD DESC:Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism Gene:PEP7(YDR323C)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PEX28(YHR150W)|FD-Score:4.89|P-value:4.92E-7||SGD DESC:Peroxisomal integral membrane peroxin, involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p Gene:PEX32(YBR168W)|FD-Score:-3.12|P-value:9.20E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PHO87(YCR037C)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication Gene:PUS4(YNL292W)|FD-Score:4.53|P-value:3.01E-6||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype) Gene:RCY1(YJL204C)|FD-Score:14.1|P-value:1.59E-45||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:REI1(YBR267W)|FD-Score:3.17|P-value:7.73E-4||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RGP1(YDR137W)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIC1(YLR039C)|FD-Score:-3.93|P-value:4.26E-5||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPL23B(YER117W)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Ribosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication Gene:RPL2A(YFR031C-A)|FD-Score:-3.72|P-value:9.90E-5||SGD DESC:Ribosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication Gene:RPL6B(YLR448W)|FD-Score:-4.18|P-value:1.44E-5||SGD DESC:Ribosomal 60S subunit protein L6B; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6B has a paralog, RPL6A, that arose from the whole genome duplication Gene:RRG9(YNL213C)|FD-Score:-4.35|P-value:6.82E-6||SGD DESC:Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RTS3(YGR161C_p)|FD-Score:3.95|P-value:3.84E-5||SGD DESC:Putative component of the protein phosphatase type 2A complex Gene:SLG1(YOR008C)|FD-Score:-3.22|P-value:6.34E-4||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SNT309(YPR101W)|FD-Score:-3.8|P-value:7.32E-5||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SOL2(YCR073W-A)|FD-Score:4.75|P-value:1.01E-6||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:SPH1(YLR313C)|FD-Score:4.32|P-value:7.78E-6||SGD DESC:Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p Gene:SPT3(YDR392W)|FD-Score:-5.31|P-value:5.63E-8||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:STP3(YLR375W)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Zinc-finger protein of unknown function, possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids Gene:SWI3(YJL176C)|FD-Score:-3.43|P-value:3.05E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions Gene:TRP1(YDR007W)|FD-Score:8.76|P-value:1.00E-18||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP3(YKL211C)|FD-Score:4.52|P-value:3.12E-6||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:ULS1(YOR191W)|FD-Score:5.38|P-value:3.66E-8||SGD DESC:Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:UPC2(YDR213W)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Sterol regulatory element binding protein; induces transcription of sterol biosynthetic genes and of DAN/TIR gene products; relocates from intracellular membranes to perinuclear foci on sterol depletion; UPC2 has a paralog, ECM22, that arose from the whole genome duplication Gene:USA1(YML029W)|FD-Score:3.29|P-value:5.00E-4||SGD DESC:Scaffold subunit of the Hrd1p ubiquitin ligase that also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p Gene:VMA22(YHR060W)|FD-Score:-4.22|P-value:1.21E-5||SGD DESC:Peripheral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) Gene:VPS3(YDR495C)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS61(YDR136C_d)|FD-Score:-4.35|P-value:6.90E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YAL065C(YAL065C_p)|FD-Score:4.89|P-value:5.07E-7||SGD DESC:Putative protein of unknown function; has homology to FLO1; possible pseudogene Gene:YBR232C(YBR232C_d)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCL022C(YCL022C_d)|FD-Score:-3.12|P-value:8.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene KCC4/YCL024W Gene:YDC1(YPL087W)|FD-Score:3.84|P-value:6.22E-5||SGD DESC:Alkaline dihydroceramidase, involved in sphingolipid metabolism; preferentially hydrolyzes dihydroceramide to a free fatty acid and dihydrosphingosine; has a minor reverse activity; YDC1 has a paralog, YPC1, that arose from the whole genome duplication Gene:YDL187C(YDL187C_d)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR161W(YDR161W_p)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YER119C-A(YER119C-A_d)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YFR039C(YFR039C_p)|FD-Score:-4.34|P-value:7.01E-6||SGD DESC:Putative protein of unknown function; may be involved in response to high salt and changes in carbon source; deletion mutant has decreased spore survival in Drosophila feces Gene:YGL072C(YGL072C_d)|FD-Score:5.08|P-value:1.89E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGR111W(YGR111W_p)|FD-Score:3.2|P-value:6.86E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YGR237C(YGR237C_p)|FD-Score:-3.23|P-value:6.18E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YHR033W(YHR033W_p)|FD-Score:-3.74|P-value:9.28E-5||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YHR033W has a paralog, PRO1, that arose from the whole genome duplication Gene:YIR024C(YIR024C)|FD-Score:4.49|P-value:3.61E-6||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; interacts with Arh1p, a mitochondrial oxidoreductase; deletion mutant has a respiratory growth defect Gene:YKE4(YIL023C)|FD-Score:-3.51|P-value:2.28E-4||SGD DESC:Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family Gene:YLL044W(YLL044W_d)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLL044W and the overlapping gene RPL8B is reduced in the gcr1 null mutant Gene:YLL047W(YLL047W_d)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RNP1 Gene:YLR211C(YLR211C_p)|FD-Score:-3.65|P-value:1.33E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR211C is not an essential gene; ORF contains an intron Gene:YLR225C(YLR225C_p)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene Gene:YML007C-A(YML007C-A_p)|FD-Score:3.78|P-value:7.83E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YML018C(YML018C_p)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication Gene:YML122C(YML122C_d)|FD-Score:-3.88|P-value:5.20E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR027W(YMR027W_p)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR027W is not an essential gene Gene:YMR242W-A(YMR242W-A_p)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Putative protein of unknown function Gene:YNL184C(YNL184C_p)|FD-Score:-4.44|P-value:4.42E-6||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOL046C(YOL046C_d)|FD-Score:-3.45|P-value:2.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data;almost completely overlaps the verified gene PSK2/YOL045W Gene:YOR041C(YOR041C_d)|FD-Score:-4.88|P-value:5.24E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the uncharacterized gene CUE5; deletion in cyr1 mutant results in loss of stress resistance Gene:YOR082C(YOR082C_d)|FD-Score:-3.61|P-value:1.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene YOR083W Gene:YOR366W(YOR366W_d)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C Gene:YOR385W(YOR385W_p)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:YPK1(YKL126W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL182C(YPL182C_d)|FD-Score:-3.6|P-value:1.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W Gene:YPL260W(YPL260W_p)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Putative substrate of cAMP-dependent protein kinase (PKA); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YPL260W is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YPR077C(YPR077C_d)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1 Gene:YPS6(YIR039C)|FD-Score:3.94|P-value:4.09E-5||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance Gene:YPT31(YER031C)|FD-Score:3.77|P-value:8.30E-5||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:YPT6(YLR262C)|FD-Score:-4.4|P-value:5.53E-6||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YSC84(YHR016C)|FD-Score:3.19|P-value:7.12E-4||SGD DESC:Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL055W9.441.82E-210.82OLE1Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
YLR009W8.873.71E-190.82RLP24Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
YMR203W8.054.30E-160.30TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YNR053C7.754.72E-150.61NOG2Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation
YBR190W_d7.144.74E-130.14YBR190W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W
YDR047W7.001.31E-120.73HEM12Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
YJR007W6.271.85E-100.63SUI2Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress
YOR206W5.648.73E-90.26NOC2Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors
YDL196W_d5.383.82E-80.15YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YGL030W5.238.49E-80.12RPL30Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog
YOL144W5.111.61E-70.21NOP8Nucleolar protein required for 60S ribosomal subunit biogenesis
YOR319W4.904.73E-70.41HSH49U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
YMR290W-A_d4.503.44E-60.01YMR290W-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase
YJR123W4.483.68E-60.10RPS5Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7
YBL105C4.385.84E-60.03PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDL226C25.404.49E-143GCS1ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p
YJL204C14.101.59E-45RCY1F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
YCR094W13.301.66E-40CDC50Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication
YGL148W9.431.97E-21ARO2Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress
YPR139C8.981.37E-19LOA1Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA
YDR007W8.761.00E-18TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YHR132C6.388.60E-11ECM14Putative metalloprotease with similarity to the zinc carboxypeptidase family, required for normal cell wall assembly
YJR060W5.422.99E-8CBF1Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress
YOR191W5.383.66E-8ULS1Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress
YGL072C_d5.081.89E-7YGL072C_dDubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2
YHR150W4.894.92E-7PEX28Peroxisomal integral membrane peroxin, involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p
YAL065C_p4.895.07E-7YAL065C_pPutative protein of unknown function; has homology to FLO1; possible pseudogene
YKR077W4.779.43E-7MSA2Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation
YAL026C4.769.48E-7DRS2Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease
YCR073W-A4.751.01E-6SOL2Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication

GO enrichment analysis for SGTC_2887
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.5170SGTC_30819117243 49.5 μMChembridge (Drug-like library)455955890.153846fatty acid desaturase (OLE1)
0.4660SGTC_28809042983 58.4 μMChembridge (Drug-like library)64647140.666667fatty acid desaturase (OLE1)
0.4517.85E-294SGTC_258haloperidol 50.8 μMMiscellaneous35590.157143fatty acid desaturase (OLE1)
0.4368.85E-273SGTC_31269127922 49.5 μMChembridge (Drug-like library)455956820.147059fatty acid desaturase (OLE1)
0.4032.70E-229SGTC_29939054257 71.4 μMChembridge (Drug-like library)64702170.761905fatty acid desaturase (OLE1)
0.4033.49E-229SGTC_28389002687 71.4 μMChembridge (Drug-like library)64570600.568627fatty acid desaturase (OLE1)
0.4011.65E-226SGTC_20505263056 171.0 μMChembridge (Fragment library)7634680.375fatty acid desaturase (OLE1)
0.3941.20E-218SGTC_30719117417 49.5 μMChembridge (Drug-like library)455955900.180328fatty acid desaturase (OLE1)
0.3931.16E-216SGTC_218alverine citrate 93.8 μMMiscellaneous217180.176471fatty acid desaturase (OLE1)
0.3858.49E-208SGTC_28939048488 26.0 μMChembridge (Drug-like library)64673380.727273fatty acid desaturase (OLE1)
0.3712.36E-192SGTC_2725haloperidol 53.2 μMMiscellaneous35590.157143plasma membrane duress
0.3671.23E-187SGTC_2721ifenprodil 61.4 μMMiscellaneous36890.1875plasma membrane duress
0.3582.06E-177SGTC_2784092-0821 23.9 μMChemDiv (Drug-like library)8779000.347826plasma membrane duress
0.3434.12E-163SGTC_8870885-0007 9.6 μMChemDiv (Drug-like library)57194580.179104endomembrane recycling
0.3393.09E-158SGTC_14354092-0789 56.7 μMChemDiv (Drug-like library)22482850.327273plasma membrane duress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2993905425771.43 μM0.7619056470217Chembridge (Drug-like library)343.850483.16113fatty acid desaturase (OLE1)
SGTC_2893904848825.97 μM0.7272736467338Chembridge (Drug-like library)337.458583.46913fatty acid desaturase (OLE1)
SGTC_2880904298358.44 μM0.6666676464714Chembridge (Drug-like library)341.4224633.18914fatty acid desaturase (OLE1)
SGTC_2895905444758.44 μM0.5744682697450Chembridge (Drug-like library)343.850483.16113
SGTC_2838900268771.43 μM0.5686276457060Chembridge (Drug-like library)357.877063.64713fatty acid desaturase (OLE1)
SGTC_14554262-0120131 μM0.4878051529587ChemDiv (Drug-like library)308.460424.87302plasma membrane duress
SGTC_3158910000749.47 μM0.4528319297233Chembridge (Drug-like library)373.286844.01902
SGTC_6164092-0450108 μM0.44787165ChemDiv (Drug-like library)322.443943.76202
SGTC_2896905265671.43 μM0.4385966469436Chembridge (Drug-like library)369.887763.62913endomembrane recycling
SGTC_1825st05214362.6 μM0.431373674173TimTec (Natural product derivative library)319.44334.22412