9059656

2,3,4,5-tetrafluoro-N-(3-fluorophenyl)benzamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2889
Screen concentration 52.0 μM
Source Chembridge (Drug-like library)
PubChem CID 3949838
SMILES C1=CC(=CC(=C1)F)NC(=O)C2=CC(=C(C(=C2F)F)F)F
Standardized SMILES Fc1cccc(NC(=O)c2cc(F)c(F)c(F)c2F)c1
Molecular weight 287.1849
ALogP 3.64
H-bond donor count 1
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.26
% growth inhibition (Hom. pool) 5.38


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3949838
Download HIP data (tab-delimited text)  (excel)
Gene:DYS1(YHR068W)|FD-Score:-5.05|P-value:2.17E-7|Clearance:0||SGD DESC:Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric Gene:NUP49(YGL172W)|FD-Score:-3.37|P-value:3.76E-4|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:PUP2(YGR253C)|FD-Score:3.51|P-value:2.26E-4|Clearance:0.24||SGD DESC:Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta Gene:RER2(YBR002C)|FD-Score:6.13|P-value:4.43E-10|Clearance:1.7||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RFA3(YJL173C)|FD-Score:4.43|P-value:4.66E-6|Clearance:0.93||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress Gene:RPS15(YOL040C)|FD-Score:3.1|P-value:9.69E-4|Clearance:0.02||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:SPC19(YDR201W)|FD-Score:-3.35|P-value:4.08E-4|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body Gene:UFD1(YGR048W)|FD-Score:3.26|P-value:5.49E-4|Clearance:0.16||SGD DESC:Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP) Gene:USE1(YGL098W)|FD-Score:-3.72|P-value:9.95E-5|Clearance:0||SGD DESC:Essential SNARE protein localized to the ER, involved in retrograde traffic from the Golgi to the ER; forms a complex with the SNAREs Sec22p, Sec20p and Ufe1p Gene:DYS1(YHR068W)|FD-Score:-5.05|P-value:2.17E-7|Clearance:0||SGD DESC:Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric Gene:NUP49(YGL172W)|FD-Score:-3.37|P-value:3.76E-4|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:PUP2(YGR253C)|FD-Score:3.51|P-value:2.26E-4|Clearance:0.24||SGD DESC:Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta Gene:RER2(YBR002C)|FD-Score:6.13|P-value:4.43E-10|Clearance:1.7||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RFA3(YJL173C)|FD-Score:4.43|P-value:4.66E-6|Clearance:0.93||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress Gene:RPS15(YOL040C)|FD-Score:3.1|P-value:9.69E-4|Clearance:0.02||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:SPC19(YDR201W)|FD-Score:-3.35|P-value:4.08E-4|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body Gene:UFD1(YGR048W)|FD-Score:3.26|P-value:5.49E-4|Clearance:0.16||SGD DESC:Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP) Gene:USE1(YGL098W)|FD-Score:-3.72|P-value:9.95E-5|Clearance:0||SGD DESC:Essential SNARE protein localized to the ER, involved in retrograde traffic from the Golgi to the ER; forms a complex with the SNAREs Sec22p, Sec20p and Ufe1p

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3949838
Download HOP data (tab-delimited text)  (excel)
Gene:ATG15(YCR068W)|FD-Score:-3.13|P-value:8.68E-4||SGD DESC:Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway Gene:ATS1(YAL020C)|FD-Score:-3.37|P-value:3.70E-4||SGD DESC:Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle Gene:AVT2(YEL064C)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:AZR1(YGR224W)|FD-Score:-3.3|P-value:4.75E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily, involved in resistance to azole drugs such as ketoconazole and fluconazole Gene:BSC4(YNL269W)|FD-Score:4.03|P-value:2.81E-5||SGD DESC:Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p Gene:BUD23(YCR047C)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:Methyltransferase, methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern Gene:CLB5(YPR120C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase Gene:ECM25(YJL201W)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Non-essential protein of unknown function; promoter contains a consensus binding sequence for factor Abf1p Gene:GLY1(YEL046C)|FD-Score:4.36|P-value:6.62E-6||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:LCL1(YPL056C_p)|FD-Score:3.79|P-value:7.48E-5||SGD DESC:Putative protein of unknown function; deletion mutant is fluconazole resistant and has long chronological lifespan Gene:LSC2(YGR244C)|FD-Score:3.18|P-value:7.40E-4||SGD DESC:Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate Gene:MAG2(YLR427W)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Cytoplasmic protein of unknown function; induced in response to mycotoxin patulin; ubiquitinated protein similar to the human ring finger motif protein RNF10; predicted to be involved in repair of alkylated DNA due to interaction with MAG1 Gene:MCM21(YDR318W)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:MSS1(YMR023C)|FD-Score:5.22|P-value:9.12E-8||SGD DESC:Mitochondrial protein, forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; similar to human GTPBP3 Gene:NMA111(YNL123W)|FD-Score:5.05|P-value:2.18E-7||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:PBS2(YJL128C)|FD-Score:3.76|P-value:8.43E-5||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PHO13(YDL236W)|FD-Score:-3.57|P-value:1.81E-4||SGD DESC:Alkaline phosphatase specific for p-nitrophenyl phosphate; also has protein phosphatase activity Gene:RAD61(YDR014W)|FD-Score:3.86|P-value:5.75E-5||SGD DESC:Subunit of a complex (Scc3p, Pds5p, Rad61p) that inhibits sister chromatid cohesion; inhibited by Eco1p-acetylated cohesin subunits Smc3p and Mcd1p; related to the human Wapl protein that controls the association of cohesin with chromatin Gene:RIM101(YHL027W)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RPP2B(YDR382W)|FD-Score:-3.38|P-value:3.65E-4||SGD DESC:Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:RTS1(YOR014W)|FD-Score:4.32|P-value:7.94E-6||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:SEO1(YAL067C)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Putative permease, member of the allantoate transporter subfamily of the major facilitator superfamily; mutation confers resistance to ethionine sulfoxide Gene:SNU66(YOR308C)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:Component of the U4/U6.U5 snRNP complex involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1 Gene:SPO21(YOL091W)|FD-Score:-3.12|P-value:8.90E-4||SGD DESC:Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation Gene:SPO7(YAL009W)|FD-Score:-4.22|P-value:1.24E-5||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:SSZ1(YHR064C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP Gene:SUV3(YPL029W)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:TAN1(YGL232W)|FD-Score:-3.92|P-value:4.44E-5||SGD DESC:Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress Gene:TVP18(YMR071C)|FD-Score:3.11|P-value:9.31E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:URA7(YBL039C)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Major CTP synthase isozyme (see also URA8); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA7 has a paralog, URA8, that arose from the whole genome duplication Gene:YCR101C(YCR101C_p)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:-4.6|P-value:2.09E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YFL040W(YFL040W_p)|FD-Score:4.56|P-value:2.53E-6||SGD DESC:Putative transporter, member of the sugar porter family; YFL040W is not an essential gene Gene:YGR021W(YGR021W_p)|FD-Score:3.79|P-value:7.57E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YHR210C(YHR210C_p)|FD-Score:-4.25|P-value:1.07E-5||SGD DESC:Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions Gene:YLR122C(YLR122C_d)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C Gene:YML007C-A(YML007C-A_p)|FD-Score:-3.32|P-value:4.43E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YMR031W-A(YMR031W-A_d)|FD-Score:3.36|P-value:3.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Gene:YMR226C(YMR226C)|FD-Score:4.4|P-value:5.35E-6||SGD DESC:NADP(+)-dependent dehydrogenase; acts on serine, L-allo-threonine, and other 3-hydroxy acids; green fluorescent protein fusion protein localizes to the cytoplasm and nucleus; may interact with ribosomes, based on co-purification experiments Gene:YMR317W(YMR317W_p)|FD-Score:3.1|P-value:9.52E-4||SGD DESC:Putative protein of unknown function with some similarity to sialidase from Trypanosoma; YMR317W is not an essential gene Gene:YND1(YER005W)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Apyrase with wide substrate specificity, helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity Gene:YSC84(YHR016C)|FD-Score:3.83|P-value:6.35E-5||SGD DESC:Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication Gene:ATG15(YCR068W)|FD-Score:-3.13|P-value:8.68E-4||SGD DESC:Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway Gene:ATS1(YAL020C)|FD-Score:-3.37|P-value:3.70E-4||SGD DESC:Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle Gene:AVT2(YEL064C)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:AZR1(YGR224W)|FD-Score:-3.3|P-value:4.75E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily, involved in resistance to azole drugs such as ketoconazole and fluconazole Gene:BSC4(YNL269W)|FD-Score:4.03|P-value:2.81E-5||SGD DESC:Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p Gene:BUD23(YCR047C)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:Methyltransferase, methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern Gene:CLB5(YPR120C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase Gene:ECM25(YJL201W)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Non-essential protein of unknown function; promoter contains a consensus binding sequence for factor Abf1p Gene:GLY1(YEL046C)|FD-Score:4.36|P-value:6.62E-6||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:LCL1(YPL056C_p)|FD-Score:3.79|P-value:7.48E-5||SGD DESC:Putative protein of unknown function; deletion mutant is fluconazole resistant and has long chronological lifespan Gene:LSC2(YGR244C)|FD-Score:3.18|P-value:7.40E-4||SGD DESC:Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate Gene:MAG2(YLR427W)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Cytoplasmic protein of unknown function; induced in response to mycotoxin patulin; ubiquitinated protein similar to the human ring finger motif protein RNF10; predicted to be involved in repair of alkylated DNA due to interaction with MAG1 Gene:MCM21(YDR318W)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:MSS1(YMR023C)|FD-Score:5.22|P-value:9.12E-8||SGD DESC:Mitochondrial protein, forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; similar to human GTPBP3 Gene:NMA111(YNL123W)|FD-Score:5.05|P-value:2.18E-7||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:PBS2(YJL128C)|FD-Score:3.76|P-value:8.43E-5||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PHO13(YDL236W)|FD-Score:-3.57|P-value:1.81E-4||SGD DESC:Alkaline phosphatase specific for p-nitrophenyl phosphate; also has protein phosphatase activity Gene:RAD61(YDR014W)|FD-Score:3.86|P-value:5.75E-5||SGD DESC:Subunit of a complex (Scc3p, Pds5p, Rad61p) that inhibits sister chromatid cohesion; inhibited by Eco1p-acetylated cohesin subunits Smc3p and Mcd1p; related to the human Wapl protein that controls the association of cohesin with chromatin Gene:RIM101(YHL027W)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RPP2B(YDR382W)|FD-Score:-3.38|P-value:3.65E-4||SGD DESC:Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:RTS1(YOR014W)|FD-Score:4.32|P-value:7.94E-6||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:SEO1(YAL067C)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Putative permease, member of the allantoate transporter subfamily of the major facilitator superfamily; mutation confers resistance to ethionine sulfoxide Gene:SNU66(YOR308C)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:Component of the U4/U6.U5 snRNP complex involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1 Gene:SPO21(YOL091W)|FD-Score:-3.12|P-value:8.90E-4||SGD DESC:Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation Gene:SPO7(YAL009W)|FD-Score:-4.22|P-value:1.24E-5||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:SSZ1(YHR064C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP Gene:SUV3(YPL029W)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:TAN1(YGL232W)|FD-Score:-3.92|P-value:4.44E-5||SGD DESC:Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress Gene:TVP18(YMR071C)|FD-Score:3.11|P-value:9.31E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:URA7(YBL039C)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Major CTP synthase isozyme (see also URA8); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA7 has a paralog, URA8, that arose from the whole genome duplication Gene:YCR101C(YCR101C_p)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:-4.6|P-value:2.09E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YFL040W(YFL040W_p)|FD-Score:4.56|P-value:2.53E-6||SGD DESC:Putative transporter, member of the sugar porter family; YFL040W is not an essential gene Gene:YGR021W(YGR021W_p)|FD-Score:3.79|P-value:7.57E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YHR210C(YHR210C_p)|FD-Score:-4.25|P-value:1.07E-5||SGD DESC:Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions Gene:YLR122C(YLR122C_d)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C Gene:YML007C-A(YML007C-A_p)|FD-Score:-3.32|P-value:4.43E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YMR031W-A(YMR031W-A_d)|FD-Score:3.36|P-value:3.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Gene:YMR226C(YMR226C)|FD-Score:4.4|P-value:5.35E-6||SGD DESC:NADP(+)-dependent dehydrogenase; acts on serine, L-allo-threonine, and other 3-hydroxy acids; green fluorescent protein fusion protein localizes to the cytoplasm and nucleus; may interact with ribosomes, based on co-purification experiments Gene:YMR317W(YMR317W_p)|FD-Score:3.1|P-value:9.52E-4||SGD DESC:Putative protein of unknown function with some similarity to sialidase from Trypanosoma; YMR317W is not an essential gene Gene:YND1(YER005W)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Apyrase with wide substrate specificity, helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity Gene:YSC84(YHR016C)|FD-Score:3.83|P-value:6.35E-5||SGD DESC:Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR002C6.134.43E-101.70RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YJL173C4.434.66E-60.93RFA3Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress
YGR253C3.512.26E-40.24PUP2Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta
YGR048W3.265.49E-40.17UFD1Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP)
YOL040C3.109.69E-40.02RPS15Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19
YJL050W3.080.001030.06MTR4ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing
YMR128W3.020.001250.01ECM16Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis
YDL195W3.020.001270.12SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YBR070C2.900.001870.02ALG14Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases
YGL171W2.880.002000.07ROK1RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation
YIL026C2.810.002510.06IRR1Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability
YNL062C2.750.002980.08GCD10Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression
YBL018C2.670.003770.01POP8Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YPR107C2.660.003880.05YTH1Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
YDL102W2.610.004460.04POL3Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER)

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR023C5.229.12E-8MSS1Mitochondrial protein, forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; similar to human GTPBP3
YNL123W5.052.18E-7NMA111Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases
YFL040W_p4.562.53E-6YFL040W_pPutative transporter, member of the sugar porter family; YFL040W is not an essential gene
YMR226C4.405.35E-6YMR226CNADP(+)-dependent dehydrogenase; acts on serine, L-allo-threonine, and other 3-hydroxy acids; green fluorescent protein fusion protein localizes to the cytoplasm and nucleus; may interact with ribosomes, based on co-purification experiments
YEL046C4.366.62E-6GLY1Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis
YOR014W4.327.94E-6RTS1B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A
YCR047C4.111.97E-5BUD23Methyltransferase, methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern
YNL269W4.032.81E-5BSC4Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p
YDR014W3.865.75E-5RAD61Subunit of a complex (Scc3p, Pds5p, Rad61p) that inhibits sister chromatid cohesion; inhibited by Eco1p-acetylated cohesin subunits Smc3p and Mcd1p; related to the human Wapl protein that controls the association of cohesin with chromatin
YHR016C3.836.35E-5YSC84Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication
YPL056C_p3.797.48E-5LCL1_pPutative protein of unknown function; deletion mutant is fluconazole resistant and has long chronological lifespan
YGR021W_p3.797.57E-5YGR021W_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YJL128C3.768.43E-5PBS2MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition
YHR064C3.591.63E-4SSZ1Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP
YPR120C3.561.87E-4CLB5B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase

GO enrichment analysis for SGTC_2889
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1031.92E-15SGTC_28839059145 45.5 μMChembridge (Drug-like library)164765100.44
0.0912.69E-12SGTC_1863455-3465 67.3 μMChemDiv (Drug-like library)7552780.0689655
0.0897.16E-12SGTC_13281441-0025 69.2 μMChemDiv (Drug-like library)45626010.169492
0.0881.57E-11SGTC_2513baicalein 25.6 μMMicrosource (Natural product library)52816050.101695calcium & mitochondrial duress
0.0823.53E-10SGTC_2516gossypol 71.5 μMTimTec (Pure natural product library)35030.0655738
0.0781.78E-9SGTC_28959054447 58.4 μMChembridge (Drug-like library)26974500.15
0.0782.13E-9SGTC_29649083345 53.4 μMChembridge (Drug-like library)177415790.181818
0.0691.35E-7SGTC_32509135583 49.5 μMChembridge (Drug-like library)76993530.172414
0.0621.98E-6SGTC_1310141-0277 33.8 μMChemDiv (Drug-like library)15477710.272727
0.0603.40E-6SGTC_2771fluoxetine 41.0 μMMiscellaneous33860.0967742
0.0604.41E-6SGTC_1622st003707 19.4 μMTimTec (Natural product derivative library)101408990.0813954calcium & mitochondrial duress
0.0604.56E-6SGTC_28267992541 19.5 μMChembridge (Drug-like library)29850660.269231
0.0595.96E-6SGTC_20405100729 123.0 μMChembridge (Fragment library)118400.0980392
0.0588.72E-6SGTC_12600700-1617 7.8 μMChemDiv (Drug-like library)28530780.107143calcium & mitochondrial duress
0.0571.16E-5SGTC_5203584-0023 682.0 μMChemDiv (Drug-like library)13407900.09375

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_3090911769649.47 μM0.5111117923821Chembridge (Drug-like library)297.2475534.37915
SGTC_2942905277762.25 μM0.56469503Chembridge (Drug-like library)284.1131234.14912
SGTC_3115912367149.47 μM0.4666673286988Chembridge (Drug-like library)297.2475533.82115
SGTC_2883905914545.46 μM0.4416476510Chembridge (Drug-like library)269.2703833.99613
SGTC_2977901119771.43 μM0.4347832704722Chembridge (Drug-like library)279.3050463.41914
SGTC_2872904403725.97 μM0.46465195Chembridge (Drug-like library)284.1131234.14912
SGTC_2882905804458.44 μM0.418076804Chembridge (Drug-like library)273.3021433.62513
SGTC_20485246248200 μM0.391304677046Chembridge (Fragment library)216.2110631.66913
SGTC_3204911418349.47 μM0.38775517027248Chembridge (Drug-like library)329.2463533.40317
SGTC_21245246320200 μM0.386364579342Chembridge (Fragment library)216.2110631.66913heme biosynthesis & mitochondrial translocase