9048625

2-(2,6-dimethylmorpholin-4-yl)-N-(4-methoxyphenyl)acetamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2894
Screen concentration 32.5 μM
Source Chembridge (Drug-like library)
PubChem CID 4092861
SMILES CC1CN(CC(O1)C)CC(=O)NC2=CC=C(C=C2)OC
Standardized SMILES COc1ccc(NC(=O)CN2CC(C)OC(C)C2)cc1
Molecular weight 278.3468
ALogP 1.38
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0
% growth inhibition (Hom. pool) -1.23


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4092861
Download HIP data (tab-delimited text)  (excel)
Gene:ARB1(YER036C)|FD-Score:-5|P-value:2.82E-7|Clearance:0||SGD DESC:ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p Gene:ATM1(YMR301C)|FD-Score:3.31|P-value:4.64E-4|Clearance:0.12||SGD DESC:Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol Gene:CBK1(YNL161W)|FD-Score:3.19|P-value:7.00E-4|Clearance:0.03||SGD DESC:Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress Gene:DAD2(YKR083C)|FD-Score:3.67|P-value:1.19E-4|Clearance:0.07||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:DAM1(YGR113W)|FD-Score:-3.71|P-value:1.05E-4|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments Gene:DHR2(YKL078W)|FD-Score:4.61|P-value:2.01E-6|Clearance:0.27||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:EMG1(YLR186W)|FD-Score:3.99|P-value:3.31E-5|Clearance:0||SGD DESC:Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity Gene:FMN1(YDR236C)|FD-Score:4.92|P-value:4.41E-7|Clearance:0.31||SGD DESC:Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane Gene:GPI11(YDR302W)|FD-Score:4.96|P-value:3.44E-7|Clearance:0.05||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:HAS1(YMR290C)|FD-Score:3.83|P-value:6.39E-5|Clearance:0.03||SGD DESC:ATP-dependent RNA helicase; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles Gene:KRE5(YOR336W)|FD-Score:5.76|P-value:4.09E-9|Clearance:0.55||SGD DESC:Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects Gene:MAK11(YKL021C)|FD-Score:3.8|P-value:7.31E-5|Clearance:0.12||SGD DESC:Protein involved in an early, nucleolar step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats Gene:NOB1(YOR056C)|FD-Score:3.94|P-value:4.15E-5|Clearance:0.04||SGD DESC:Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:NUP49(YGL172W)|FD-Score:3.61|P-value:1.55E-4|Clearance:0.17||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:POP1(YNL221C)|FD-Score:-3.49|P-value:2.44E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:PRP2(YNR011C)|FD-Score:4.34|P-value:7.20E-6|Clearance:0.35||SGD DESC:RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16 Gene:PWP1(YLR196W)|FD-Score:5.52|P-value:1.74E-8|Clearance:0.55||SGD DESC:Protein with WD-40 repeats involved in rRNA processing; associates with trans-acting ribosome biogenesis factors; similar to beta-transducin superfamily Gene:RFA3(YJL173C)|FD-Score:-3.49|P-value:2.45E-4|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress Gene:RNR2(YJL026W)|FD-Score:-3.15|P-value:8.18E-4|Clearance:0||SGD DESC:Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits Gene:SHR3(YDL212W)|FD-Score:3.17|P-value:7.75E-4|Clearance:0.1||SGD DESC:Endoplasmic reticulum packaging chaperone, required for incorporation of amino acid permeases into COPII coated vesicles for transport to the cell surface Gene:SMD3(YLR147C)|FD-Score:3.99|P-value:3.36E-5|Clearance:0.05||SGD DESC:Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3 Gene:SPC98(YNL126W)|FD-Score:3.17|P-value:7.65E-4|Clearance:0||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:SPP2(YOR148C)|FD-Score:-4.97|P-value:3.33E-7|Clearance:0||SGD DESC:Essential protein that promotes the first step of splicing and is required for the final stages of spliceosome maturation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction Gene:SPP381(YBR152W)|FD-Score:-4.07|P-value:2.39E-5|Clearance:0||SGD DESC:mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p Gene:SSC1(YJR045C)|FD-Score:-5.17|P-value:1.16E-7|Clearance:0||SGD DESC:Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication Gene:TIF35(YDR429C)|FD-Score:-3.38|P-value:3.65E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:YLR140W(YLR140W_d)|FD-Score:3.44|P-value:2.94E-4|Clearance:0.13||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I Gene:YPD1(YDL235C)|FD-Score:3.89|P-value:4.92E-5|Clearance:0.06||SGD DESC:Phosphorelay intermediate protein, phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus Gene:YPL142C(YPL142C_d)|FD-Score:-3.5|P-value:2.36E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit Gene:ARB1(YER036C)|FD-Score:-5|P-value:2.82E-7|Clearance:0||SGD DESC:ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p Gene:ATM1(YMR301C)|FD-Score:3.31|P-value:4.64E-4|Clearance:0.12||SGD DESC:Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol Gene:CBK1(YNL161W)|FD-Score:3.19|P-value:7.00E-4|Clearance:0.03||SGD DESC:Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress Gene:DAD2(YKR083C)|FD-Score:3.67|P-value:1.19E-4|Clearance:0.07||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:DAM1(YGR113W)|FD-Score:-3.71|P-value:1.05E-4|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments Gene:DHR2(YKL078W)|FD-Score:4.61|P-value:2.01E-6|Clearance:0.27||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:EMG1(YLR186W)|FD-Score:3.99|P-value:3.31E-5|Clearance:0||SGD DESC:Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity Gene:FMN1(YDR236C)|FD-Score:4.92|P-value:4.41E-7|Clearance:0.31||SGD DESC:Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane Gene:GPI11(YDR302W)|FD-Score:4.96|P-value:3.44E-7|Clearance:0.05||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:HAS1(YMR290C)|FD-Score:3.83|P-value:6.39E-5|Clearance:0.03||SGD DESC:ATP-dependent RNA helicase; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles Gene:KRE5(YOR336W)|FD-Score:5.76|P-value:4.09E-9|Clearance:0.55||SGD DESC:Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects Gene:MAK11(YKL021C)|FD-Score:3.8|P-value:7.31E-5|Clearance:0.12||SGD DESC:Protein involved in an early, nucleolar step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats Gene:NOB1(YOR056C)|FD-Score:3.94|P-value:4.15E-5|Clearance:0.04||SGD DESC:Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:NUP49(YGL172W)|FD-Score:3.61|P-value:1.55E-4|Clearance:0.17||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:POP1(YNL221C)|FD-Score:-3.49|P-value:2.44E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:PRP2(YNR011C)|FD-Score:4.34|P-value:7.20E-6|Clearance:0.35||SGD DESC:RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16 Gene:PWP1(YLR196W)|FD-Score:5.52|P-value:1.74E-8|Clearance:0.55||SGD DESC:Protein with WD-40 repeats involved in rRNA processing; associates with trans-acting ribosome biogenesis factors; similar to beta-transducin superfamily Gene:RFA3(YJL173C)|FD-Score:-3.49|P-value:2.45E-4|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress Gene:RNR2(YJL026W)|FD-Score:-3.15|P-value:8.18E-4|Clearance:0||SGD DESC:Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits Gene:SHR3(YDL212W)|FD-Score:3.17|P-value:7.75E-4|Clearance:0.1||SGD DESC:Endoplasmic reticulum packaging chaperone, required for incorporation of amino acid permeases into COPII coated vesicles for transport to the cell surface Gene:SMD3(YLR147C)|FD-Score:3.99|P-value:3.36E-5|Clearance:0.05||SGD DESC:Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3 Gene:SPC98(YNL126W)|FD-Score:3.17|P-value:7.65E-4|Clearance:0||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:SPP2(YOR148C)|FD-Score:-4.97|P-value:3.33E-7|Clearance:0||SGD DESC:Essential protein that promotes the first step of splicing and is required for the final stages of spliceosome maturation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction Gene:SPP381(YBR152W)|FD-Score:-4.07|P-value:2.39E-5|Clearance:0||SGD DESC:mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p Gene:SSC1(YJR045C)|FD-Score:-5.17|P-value:1.16E-7|Clearance:0||SGD DESC:Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication Gene:TIF35(YDR429C)|FD-Score:-3.38|P-value:3.65E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:YLR140W(YLR140W_d)|FD-Score:3.44|P-value:2.94E-4|Clearance:0.13||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I Gene:YPD1(YDL235C)|FD-Score:3.89|P-value:4.92E-5|Clearance:0.06||SGD DESC:Phosphorelay intermediate protein, phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus Gene:YPL142C(YPL142C_d)|FD-Score:-3.5|P-value:2.36E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4092861
Download HOP data (tab-delimited text)  (excel)
Gene:ADE12(YNL220W)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence Gene:AGE2(YIL044C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif Gene:ARL3(YPL051W)|FD-Score:-3.43|P-value:3.02E-4||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ATP7(YKL016C)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:ATS1(YAL020C)|FD-Score:-4.48|P-value:3.76E-6||SGD DESC:Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle Gene:BUL1(YMR275C)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex; disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases; BUL1 has a paralog, BUL2, that arose from the whole genome duplication Gene:CAM1(YPL048W)|FD-Score:-3.21|P-value:6.62E-4||SGD DESC:Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma Gene:CAP2(YIL034C)|FD-Score:6.12|P-value:4.56E-10||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:COS1(YNL336W)|FD-Score:-3.23|P-value:6.20E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:CTF4(YPR135W)|FD-Score:3.74|P-value:9.19E-5||SGD DESC:Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion Gene:CYB2(YML054C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Cytochrome b2 (L-lactate cytochrome-c oxidoreductase), component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions Gene:ECM23(YPL021W)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Non-essential protein of unconfirmed function; affects pre-rRNA processing, may act as a negative regulator of the transcription of genes involved in pseudohyphal growth; homologous to Srd1p Gene:EFM1(YHL039W)|FD-Score:3.75|P-value:8.75E-5||SGD DESC:Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:EGD2(YHR193C)|FD-Score:-3.4|P-value:3.35E-4||SGD DESC:Alpha subunit of the heteromeric nascent polypeptide-associated complex (NAC) involved in protein sorting and translocation, associated with cytoplasmic ribosomes Gene:EIS1(YMR031C)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Component of the eisosome required for proper eisosome assembly; similarity to Ykl050cp and Uso1p; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:FMT1(YBL013W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate Gene:FRT1(YOR324C)|FD-Score:-3.13|P-value:8.65E-4||SGD DESC:Tail-anchored ER membrane protein of unknown function; substrate of the phosphatase calcineurin; interacts with homolog Frt2p; promotes cell growth in stress conditions, possibly via a role in posttranslational translocation; FRT1 has a paralog, FRT2, that arose from the whole genome duplication Gene:FZO1(YBR179C)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system Gene:GCV3(YAL044C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:GOS1(YHL031C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:HNT1(YDL125C)|FD-Score:-3.51|P-value:2.26E-4||SGD DESC:Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress Gene:KRE6(YPR159W)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:Type II integral membrane protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to the ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p Gene:KSP1(YHR082C)|FD-Score:-3.96|P-value:3.81E-5||SGD DESC:Serine/threonine protein kinase; associates with TORC1 and likely involved in TOR signaling cascades; negative regulator of autophagy; nuclear translocation required for haploid filamentous growth; regulates filamentous growth induced nuclear translocation of Bcy1p, Fus3p, and Sks1p; overproduction causes allele-specific suppression of prp20-10; protein abundance increases in response to DNA replication stress Gene:KSS1(YGR040W)|FD-Score:-4.13|P-value:1.80E-5||SGD DESC:Mitogen-activated protein kinase (MAPK) involved in signal transduction pathways that control filamentous growth and pheromone response; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains Gene:LSM6(YDR378C)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MCH4(YOL119C)|FD-Score:-3.54|P-value:1.98E-4||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MRPL13(YKR006C)|FD-Score:-3.11|P-value:9.43E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MRPL51(YPR100W)|FD-Score:-4.13|P-value:1.83E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSC7(YHR039C)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids Gene:MSS1(YMR023C)|FD-Score:-3.36|P-value:3.90E-4||SGD DESC:Mitochondrial protein, forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; similar to human GTPBP3 Gene:MSW1(YDR268W)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:NUR1(YDL089W)|FD-Score:-3.41|P-value:3.23E-4||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:PFK2(YMR205C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PMD1(YER132C)|FD-Score:-5.7|P-value:6.01E-9||SGD DESC:Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions Gene:POP2(YNR052C)|FD-Score:-4.88|P-value:5.19E-7||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PPG1(YNR032W)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:Putative serine/threonine protein phosphatase of the type 2A-like phosphatase family, required for glycogen accumulation; interacts with Tap42p, which binds to and regulates other protein phosphatases Gene:PRR2(YDL214C)|FD-Score:4.23|P-value:1.18E-5||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor; mutant has increased aneuploidy tolerance Gene:PTC7(YHR076W)|FD-Score:-4.68|P-value:1.47E-6||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:PTM1(YKL039W)|FD-Score:-3.77|P-value:8.26E-5||SGD DESC:Protein of unknown function; copurifies with late Golgi vesicles containing the v-SNARE Tlg2p; PTM1 has a paralog, YHL017W, that arose from the whole genome duplication Gene:RAX2(YLR084C)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p Gene:RED1(YLR263W)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Protein component of the synaptonemal complex axial elements, involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; interacts with Hop1p, Mec3p and Ddc1p Gene:RPL36A(YMR194W)|FD-Score:4.84|P-value:6.35E-7||SGD DESC:Ribosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication Gene:RRT12(YCR045C)|FD-Score:3.13|P-value:8.76E-4||SGD DESC:Probable subtilisin-family protease with a role in formation of the dityrosine layer of spore walls; localizes to the spore wall and also the nuclear envelope and ER region in mature spores Gene:SAN1(YDR143C)|FD-Score:3.95|P-value:3.90E-5||SGD DESC:Ubiquitin-protein ligase; involved in the proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity containing 5 or more contiguous hydrophobic residues; contains intrinsically disordered regions that contribute to substrate recognition Gene:SEO1(YAL067C)|FD-Score:-3.19|P-value:7.10E-4||SGD DESC:Putative permease, member of the allantoate transporter subfamily of the major facilitator superfamily; mutation confers resistance to ethionine sulfoxide Gene:SER3(YER081W)|FD-Score:3.13|P-value:8.88E-4||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER3 has a paralog, SER33, that arose from the whole genome duplication Gene:SHS1(YDL225W)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins; undergoes sumoylation and phosphorylation during mitosis; protein abundance increases in response to DNA replication stress Gene:SLI15(YBR156C)|FD-Score:3.82|P-value:6.79E-5||SGD DESC:Subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), which regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly Gene:SPH1(YLR313C)|FD-Score:3.12|P-value:8.99E-4||SGD DESC:Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p Gene:STB2(YMR053C)|FD-Score:3.15|P-value:8.06E-4||SGD DESC:Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p Gene:TIF2(YJL138C)|FD-Score:4.03|P-value:2.79E-5||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication Gene:VHS1(YDR247W)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Cytoplasmic serine/threonine protein kinase; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; homolog of Sks1p Gene:VPS20(YMR077C)|FD-Score:-3.1|P-value:9.70E-4||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:YAL037W(YAL037W_p)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Putative protein of unknown function; YAL037W has a paralog, YOR342C, that arose from the whole genome duplication Gene:YAL065C(YAL065C_p)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Putative protein of unknown function; has homology to FLO1; possible pseudogene Gene:YDR161W(YDR161W_p)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YFL040W(YFL040W_p)|FD-Score:3.89|P-value:5.04E-5||SGD DESC:Putative transporter, member of the sugar porter family; YFL040W is not an essential gene Gene:YGL109W(YGL109W_d)|FD-Score:3.92|P-value:4.35E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized gene YGL108C Gene:YGL159W(YGL159W_p)|FD-Score:-3.16|P-value:7.78E-4||SGD DESC:Putative protein of unknown function; deletion mutant has no detectable phenotype Gene:YGR283C(YGR283C)|FD-Score:5.43|P-value:2.84E-8||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; may interact with ribosomes, based on co-purification experiments; predicted to be involved in ribosome biogenesis; null mutant is resistant to fluconazole; GFP-fusion protein localizes to the nucleolus Gene:YMR031W-A(YMR031W-A_d)|FD-Score:4.11|P-value:1.99E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Gene:YMR175W-A(YMR175W-A_p)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Putative protein of unknown function Gene:YNL217W(YNL217W_p)|FD-Score:5.2|P-value:9.75E-8||SGD DESC:Putative protein of unknown function; weak sequence similarity to bis (5'-nucleotidyl)-tetraphosphatases; (GFP)-fusion protein localizes to the vacuole; null mutant is highly sensitive to azaserine and resistant to sodium-O-vandate Gene:YNL277W-A(YNL277W-A_p)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Putative protein of unknown function Gene:YPS3(YLR121C)|FD-Score:-3.44|P-value:2.95E-4||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:YRB30(YGL164C)|FD-Score:-3.16|P-value:7.91E-4||SGD DESC:RanGTP-binding protein, inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes Gene:ADE12(YNL220W)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence Gene:AGE2(YIL044C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif Gene:ARL3(YPL051W)|FD-Score:-3.43|P-value:3.02E-4||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ATP7(YKL016C)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:ATS1(YAL020C)|FD-Score:-4.48|P-value:3.76E-6||SGD DESC:Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle Gene:BUL1(YMR275C)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex; disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases; BUL1 has a paralog, BUL2, that arose from the whole genome duplication Gene:CAM1(YPL048W)|FD-Score:-3.21|P-value:6.62E-4||SGD DESC:Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma Gene:CAP2(YIL034C)|FD-Score:6.12|P-value:4.56E-10||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:COS1(YNL336W)|FD-Score:-3.23|P-value:6.20E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:CTF4(YPR135W)|FD-Score:3.74|P-value:9.19E-5||SGD DESC:Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion Gene:CYB2(YML054C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Cytochrome b2 (L-lactate cytochrome-c oxidoreductase), component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions Gene:ECM23(YPL021W)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Non-essential protein of unconfirmed function; affects pre-rRNA processing, may act as a negative regulator of the transcription of genes involved in pseudohyphal growth; homologous to Srd1p Gene:EFM1(YHL039W)|FD-Score:3.75|P-value:8.75E-5||SGD DESC:Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:EGD2(YHR193C)|FD-Score:-3.4|P-value:3.35E-4||SGD DESC:Alpha subunit of the heteromeric nascent polypeptide-associated complex (NAC) involved in protein sorting and translocation, associated with cytoplasmic ribosomes Gene:EIS1(YMR031C)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Component of the eisosome required for proper eisosome assembly; similarity to Ykl050cp and Uso1p; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:FMT1(YBL013W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate Gene:FRT1(YOR324C)|FD-Score:-3.13|P-value:8.65E-4||SGD DESC:Tail-anchored ER membrane protein of unknown function; substrate of the phosphatase calcineurin; interacts with homolog Frt2p; promotes cell growth in stress conditions, possibly via a role in posttranslational translocation; FRT1 has a paralog, FRT2, that arose from the whole genome duplication Gene:FZO1(YBR179C)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system Gene:GCV3(YAL044C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:GOS1(YHL031C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:HNT1(YDL125C)|FD-Score:-3.51|P-value:2.26E-4||SGD DESC:Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress Gene:KRE6(YPR159W)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:Type II integral membrane protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to the ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p Gene:KSP1(YHR082C)|FD-Score:-3.96|P-value:3.81E-5||SGD DESC:Serine/threonine protein kinase; associates with TORC1 and likely involved in TOR signaling cascades; negative regulator of autophagy; nuclear translocation required for haploid filamentous growth; regulates filamentous growth induced nuclear translocation of Bcy1p, Fus3p, and Sks1p; overproduction causes allele-specific suppression of prp20-10; protein abundance increases in response to DNA replication stress Gene:KSS1(YGR040W)|FD-Score:-4.13|P-value:1.80E-5||SGD DESC:Mitogen-activated protein kinase (MAPK) involved in signal transduction pathways that control filamentous growth and pheromone response; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains Gene:LSM6(YDR378C)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MCH4(YOL119C)|FD-Score:-3.54|P-value:1.98E-4||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MRPL13(YKR006C)|FD-Score:-3.11|P-value:9.43E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MRPL51(YPR100W)|FD-Score:-4.13|P-value:1.83E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSC7(YHR039C)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids Gene:MSS1(YMR023C)|FD-Score:-3.36|P-value:3.90E-4||SGD DESC:Mitochondrial protein, forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; similar to human GTPBP3 Gene:MSW1(YDR268W)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:NUR1(YDL089W)|FD-Score:-3.41|P-value:3.23E-4||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:PFK2(YMR205C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PMD1(YER132C)|FD-Score:-5.7|P-value:6.01E-9||SGD DESC:Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions Gene:POP2(YNR052C)|FD-Score:-4.88|P-value:5.19E-7||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PPG1(YNR032W)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:Putative serine/threonine protein phosphatase of the type 2A-like phosphatase family, required for glycogen accumulation; interacts with Tap42p, which binds to and regulates other protein phosphatases Gene:PRR2(YDL214C)|FD-Score:4.23|P-value:1.18E-5||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor; mutant has increased aneuploidy tolerance Gene:PTC7(YHR076W)|FD-Score:-4.68|P-value:1.47E-6||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:PTM1(YKL039W)|FD-Score:-3.77|P-value:8.26E-5||SGD DESC:Protein of unknown function; copurifies with late Golgi vesicles containing the v-SNARE Tlg2p; PTM1 has a paralog, YHL017W, that arose from the whole genome duplication Gene:RAX2(YLR084C)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p Gene:RED1(YLR263W)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Protein component of the synaptonemal complex axial elements, involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; interacts with Hop1p, Mec3p and Ddc1p Gene:RPL36A(YMR194W)|FD-Score:4.84|P-value:6.35E-7||SGD DESC:Ribosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication Gene:RRT12(YCR045C)|FD-Score:3.13|P-value:8.76E-4||SGD DESC:Probable subtilisin-family protease with a role in formation of the dityrosine layer of spore walls; localizes to the spore wall and also the nuclear envelope and ER region in mature spores Gene:SAN1(YDR143C)|FD-Score:3.95|P-value:3.90E-5||SGD DESC:Ubiquitin-protein ligase; involved in the proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity containing 5 or more contiguous hydrophobic residues; contains intrinsically disordered regions that contribute to substrate recognition Gene:SEO1(YAL067C)|FD-Score:-3.19|P-value:7.10E-4||SGD DESC:Putative permease, member of the allantoate transporter subfamily of the major facilitator superfamily; mutation confers resistance to ethionine sulfoxide Gene:SER3(YER081W)|FD-Score:3.13|P-value:8.88E-4||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER3 has a paralog, SER33, that arose from the whole genome duplication Gene:SHS1(YDL225W)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins; undergoes sumoylation and phosphorylation during mitosis; protein abundance increases in response to DNA replication stress Gene:SLI15(YBR156C)|FD-Score:3.82|P-value:6.79E-5||SGD DESC:Subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), which regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly Gene:SPH1(YLR313C)|FD-Score:3.12|P-value:8.99E-4||SGD DESC:Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p Gene:STB2(YMR053C)|FD-Score:3.15|P-value:8.06E-4||SGD DESC:Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p Gene:TIF2(YJL138C)|FD-Score:4.03|P-value:2.79E-5||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication Gene:VHS1(YDR247W)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Cytoplasmic serine/threonine protein kinase; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; homolog of Sks1p Gene:VPS20(YMR077C)|FD-Score:-3.1|P-value:9.70E-4||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:YAL037W(YAL037W_p)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Putative protein of unknown function; YAL037W has a paralog, YOR342C, that arose from the whole genome duplication Gene:YAL065C(YAL065C_p)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Putative protein of unknown function; has homology to FLO1; possible pseudogene Gene:YDR161W(YDR161W_p)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YFL040W(YFL040W_p)|FD-Score:3.89|P-value:5.04E-5||SGD DESC:Putative transporter, member of the sugar porter family; YFL040W is not an essential gene Gene:YGL109W(YGL109W_d)|FD-Score:3.92|P-value:4.35E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized gene YGL108C Gene:YGL159W(YGL159W_p)|FD-Score:-3.16|P-value:7.78E-4||SGD DESC:Putative protein of unknown function; deletion mutant has no detectable phenotype Gene:YGR283C(YGR283C)|FD-Score:5.43|P-value:2.84E-8||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; may interact with ribosomes, based on co-purification experiments; predicted to be involved in ribosome biogenesis; null mutant is resistant to fluconazole; GFP-fusion protein localizes to the nucleolus Gene:YMR031W-A(YMR031W-A_d)|FD-Score:4.11|P-value:1.99E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Gene:YMR175W-A(YMR175W-A_p)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Putative protein of unknown function Gene:YNL217W(YNL217W_p)|FD-Score:5.2|P-value:9.75E-8||SGD DESC:Putative protein of unknown function; weak sequence similarity to bis (5'-nucleotidyl)-tetraphosphatases; (GFP)-fusion protein localizes to the vacuole; null mutant is highly sensitive to azaserine and resistant to sodium-O-vandate Gene:YNL277W-A(YNL277W-A_p)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Putative protein of unknown function Gene:YPS3(YLR121C)|FD-Score:-3.44|P-value:2.95E-4||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:YRB30(YGL164C)|FD-Score:-3.16|P-value:7.91E-4||SGD DESC:RanGTP-binding protein, inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR336W5.764.09E-90.55KRE5Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects
YLR196W5.521.74E-80.55PWP1Protein with WD-40 repeats involved in rRNA processing; associates with trans-acting ribosome biogenesis factors; similar to beta-transducin superfamily
YDR302W4.963.44E-70.05GPI11ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog
YDR236C4.924.41E-70.31FMN1Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane
YKL078W4.612.01E-60.27DHR2Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis
YNR011C4.347.20E-60.35PRP2RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16
YLR186W3.993.31E-50.00EMG1Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity
YLR147C3.993.36E-50.05SMD3Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3
YOR056C3.944.15E-50.04NOB1Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress
YDL235C3.894.92E-50.06YPD1Phosphorelay intermediate protein, phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus
YMR290C3.836.39E-50.03HAS1ATP-dependent RNA helicase; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles
YKL021C3.807.31E-50.12MAK11Protein involved in an early, nucleolar step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats
YKR083C3.671.19E-40.07DAD2Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
YGL172W3.611.55E-40.17NUP49FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p)
YLR140W_d3.442.94E-40.12YLR140W_dDubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YIL034C6.124.56E-10CAP2Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress
YGR283C5.432.84E-8YGR283CPutative S-adenosylmethionine-dependent methyltransferase; may interact with ribosomes, based on co-purification experiments; predicted to be involved in ribosome biogenesis; null mutant is resistant to fluconazole; GFP-fusion protein localizes to the nucleolus
YNL217W_p5.209.75E-8YNL217W_pPutative protein of unknown function; weak sequence similarity to bis (5'-nucleotidyl)-tetraphosphatases; (GFP)-fusion protein localizes to the vacuole; null mutant is highly sensitive to azaserine and resistant to sodium-O-vandate
YMR194W4.846.35E-7RPL36ARibosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication
YKL016C4.241.11E-5ATP7Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis
YDL214C4.231.18E-5PRR2Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor; mutant has increased aneuploidy tolerance
YLR263W4.221.22E-5RED1Protein component of the synaptonemal complex axial elements, involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; interacts with Hop1p, Mec3p and Ddc1p
YMR031W-A_d4.111.99E-5YMR031W-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C
YJL138C4.032.79E-5TIF2Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication
YDR143C3.953.90E-5SAN1Ubiquitin-protein ligase; involved in the proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity containing 5 or more contiguous hydrophobic residues; contains intrinsically disordered regions that contribute to substrate recognition
YGL109W_d3.924.35E-5YGL109W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized gene YGL108C
YFL040W_p3.895.04E-5YFL040W_pPutative transporter, member of the sugar porter family; YFL040W is not an essential gene
YBR156C3.826.79E-5SLI15Subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), which regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly
YHL039W3.758.75E-5EFM1Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YPR135W3.749.19E-5CTF4Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion

GO enrichment analysis for SGTC_2894
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0831.68E-10SGTC_28919063876 19.5 μMChembridge (Drug-like library)64724780.185714
0.0791.12E-9SGTC_476mead acid 10.0 μMICCB bioactive library53125310.0833333
0.0773.06E-9SGTC_32179129673 49.5 μMChembridge (Drug-like library)172483120.128571
0.0612.32E-6SGTC_28989056270 58.4 μMChembridge (Drug-like library)191546990.157895
0.0603.68E-6SGTC_21625663267 200.0 μMChembridge (Fragment library)64564930.169231
0.0571.10E-5SGTC_28999053889 58.4 μMChembridge (Drug-like library)64700520.246154
0.0552.12E-5SGTC_13211326-2144 215.0 μMChemDiv (Drug-like library)35130300.0597015
0.0552.60E-5SGTC_32459134821 49.5 μMChembridge (Drug-like library)170149010.142857
0.0527.08E-5SGTC_1539benzyladenosine 56.0 μMTimTec (Pure natural product library)922080.101266
0.0501.07E-4SGTC_9073448-6986 75.5 μMChemDiv (Drug-like library)5905820.180556
0.0491.62E-4SGTC_24875918373 44.0 μMMiscellaneous28762790.178082
0.0472.65E-4SGTC_29137966556 36.6 μMChembridge (Drug-like library)29765160.19178160S ribosome export
0.0463.61E-4SGTC_169k015-0017 16.0 μMChemDiv (Drug-like library)18400020.0895522
0.0446.47E-4SGTC_2712st079126 47.7 μMTimTec (Natural product derivative library)31529700.171053
0.0447.02E-4SGTC_28959054447 58.4 μMChembridge (Drug-like library)26974500.271186

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2958907725416.72 μM0.4561416457351Chembridge (Drug-like library)292.33031.19815
SGTC_3005907649671.43 μM0.38888921368410Chembridge (Drug-like library)310.390183.67713
SGTC_2886905894451.95 μM0.370374949929Chembridge (Drug-like library)299.364243.92713
SGTC_2887904478458.44 μM0.3636366465546Chembridge (Drug-like library)323.4322.98313fatty acid desaturase (OLE1)
SGTC_3284910817164.86 μM0.3593758687505Chembridge (Drug-like library)373.876463.7214
SGTC_2835900658871.43 μM0.3571432345538Chembridge (Drug-like library)300.782683.27912
SGTC_296390825079 μM0.35483916459611Chembridge (Drug-like library)306.356881.54715
SGTC_22456637752200 μM0.354167681890Chembridge (Fragment library)199.63421.35112
SGTC_2939904457871.43 μM0.3538462653634Chembridge (Drug-like library)377.8403433.43915
SGTC_8730591-4978488 μM0.3508773305145ChemDiv (Drug-like library)300.309242.50815