9054447

2-(4-benzylpiperazin-1-yl)-N-(2-chlorophenyl)acetamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2895
Screen concentration 58.4 μM
Source Chembridge (Drug-like library)
PubChem CID 2697450
SMILES C1CN(CCN1CC2=CC=CC=C2)CC(=O)NC3=CC=CC=C3Cl
Standardized SMILES Clc1ccccc1NC(=O)CN2CCN(Cc3ccccc3)CC2
Molecular weight 343.8505
ALogP 3.16
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.08
% growth inhibition (Hom. pool) 4.49


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2697450
Download HIP data (tab-delimited text)  (excel)
Gene:AFG2(YLR397C)|FD-Score:4.68|P-value:1.40E-6|Clearance:1.13||SGD DESC:ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs Gene:NOP9(YJL010C)|FD-Score:-3.53|P-value:2.04E-4|Clearance:0||SGD DESC:Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain Gene:PDI1(YCL043C)|FD-Score:3.55|P-value:1.92E-4|Clearance:0.36||SGD DESC:Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication Gene:SSL1(YLR005W)|FD-Score:3.19|P-value:7.13E-4|Clearance:0.21||SGD DESC:Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p Gene:UBC1(YDR177W)|FD-Score:-3.43|P-value:3.04E-4|Clearance:0||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress Gene:AFG2(YLR397C)|FD-Score:4.68|P-value:1.40E-6|Clearance:1.13||SGD DESC:ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs Gene:NOP9(YJL010C)|FD-Score:-3.53|P-value:2.04E-4|Clearance:0||SGD DESC:Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain Gene:PDI1(YCL043C)|FD-Score:3.55|P-value:1.92E-4|Clearance:0.36||SGD DESC:Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication Gene:SSL1(YLR005W)|FD-Score:3.19|P-value:7.13E-4|Clearance:0.21||SGD DESC:Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p Gene:UBC1(YDR177W)|FD-Score:-3.43|P-value:3.04E-4|Clearance:0||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2697450
Download HOP data (tab-delimited text)  (excel)
Gene:AFT1(YGL071W)|FD-Score:3.8|P-value:7.17E-5||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ANB1(YJR047C)|FD-Score:3.13|P-value:8.66E-4||SGD DESC:Translation elongation factor eIF-5A; previously thought to function in translation initiation; undergoes an essential hypusination modification; expressed under anaerobic conditions; ANB1 has a paralog, HYP2, that arose from the whole genome duplication Gene:ARO1(YDR127W)|FD-Score:-4.36|P-value:6.50E-6||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO7(YPR060C)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:CTF8(YHR191C)|FD-Score:-3.6|P-value:1.58E-4||SGD DESC:Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion Gene:FYV8(YGR196C)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GLY1(YEL046C)|FD-Score:4.86|P-value:6.00E-7||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:HAP4(YKL109W)|FD-Score:-3.19|P-value:7.20E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex Gene:HMG2(YLR450W)|FD-Score:-3.42|P-value:3.12E-4||SGD DESC:HMG-CoA reductase; converts HMG-CoA to mevalonate, a rate-limiting step in sterol biosynthesis; one of two isozymes; overproduction induces assembly of peripheral ER membrane arrays and short nuclear-associated membrane stacks; forms foci at the nuclear periphery upon DNA replication stress Gene:IKS1(YJL057C)|FD-Score:-3.22|P-value:6.36E-4||SGD DESC:Protein kinase of unknown cellular role; putative serine/threonine kinase; expression is induced during mild heat stress; deletion mutants are hypersensitive to copper sulphate and resistant to sorbate; interacts with an N-terminal fragment of Sst2p Gene:LAT1(YNL071W)|FD-Score:3.78|P-value:7.95E-5||SGD DESC:Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA Gene:LSM6(YDR378C)|FD-Score:-4.95|P-value:3.67E-7||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MAD1(YGL086W)|FD-Score:4.18|P-value:1.47E-5||SGD DESC:Coiled-coil protein involved in the spindle-assembly checkpoint; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon spindle assembly checkpoint arrest; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of the activity of the anaphase promoting complex; forms a complex with Mad2p Gene:MAD3(YJL013C)|FD-Score:-3.19|P-value:7.00E-4||SGD DESC:Subunit of the spindle-assembly checkpoint complex, which delays anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover Gene:MEF2(YJL102W)|FD-Score:-3.33|P-value:4.29E-4||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:PES4(YFR023W)|FD-Score:3.27|P-value:5.34E-4||SGD DESC:Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication Gene:PKH3(YDR466W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PSY1(YKL076C_d)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C Gene:RED1(YLR263W)|FD-Score:3.76|P-value:8.42E-5||SGD DESC:Protein component of the synaptonemal complex axial elements, involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; interacts with Hop1p, Mec3p and Ddc1p Gene:RNR3(YIL066C)|FD-Score:4.81|P-value:7.52E-7||SGD DESC:Minor isoform of the large subunit of ribonucleotide-diphosphate reductase; the RNR complex catalyzes rate-limiting step in dNTP synthesis, regulated by DNA replication and DNA damage checkpoint pathways via localization of small subunits Gene:RPN13(YLR421C)|FD-Score:3.74|P-value:9.17E-5||SGD DESC:Subunit of the 19S regulatory particle of the 26S proteasome lid; acts as a ubiquitin receptor for the proteasome; null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; protein abundance increases in response to DNA replication stress Gene:SAC1(YKL212W)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAS3(YBL052C)|FD-Score:3.21|P-value:6.53E-4||SGD DESC:Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal Gene:SCO2(YBR024W)|FD-Score:4.35|P-value:6.91E-6||SGD DESC:Protein anchored to mitochondrial inner membrane; may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p; SCO2 has a paralog, SCO1, that arose from the whole genome duplication Gene:SPI1(YER150W)|FD-Score:-3.23|P-value:6.26E-4||SGD DESC:GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p Gene:SSN8(YNL025C)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance Gene:SWA2(YDR320C)|FD-Score:-3.21|P-value:6.60E-4||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:SYH1(YPL105C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:SYS1(YJL004C)|FD-Score:-3.24|P-value:5.97E-4||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:VAM6(YDL077C)|FD-Score:-3.58|P-value:1.69E-4||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VMA4(YOR332W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress Gene:YAL042C-A(YAL042C-A_d)|FD-Score:-3.18|P-value:7.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ERV46/YAL042W; YAL042C-A is a non-essential gene Gene:YDR161W(YDR161W_p)|FD-Score:3.1|P-value:9.78E-4||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YFL040W(YFL040W_p)|FD-Score:4.61|P-value:2.04E-6||SGD DESC:Putative transporter, member of the sugar porter family; YFL040W is not an essential gene Gene:YGR228W(YGR228W_d)|FD-Score:4.41|P-value:5.10E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YHR202W(YHR202W_p)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization Gene:YLR312C(YLR312C_p)|FD-Score:-3.09|P-value:9.90E-4||SGD DESC:Putative protein of unknown function Gene:YMR031W-A(YMR031W-A_d)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Gene:YMR090W(YMR090W_p)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Putative protein of unknown function with similarity to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability Gene:YNL143C(YNL143C_p)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOL037C(YOL037C_d)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOL036W Gene:YPK1(YKL126W)|FD-Score:4.52|P-value:3.07E-6||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:AFT1(YGL071W)|FD-Score:3.8|P-value:7.17E-5||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ANB1(YJR047C)|FD-Score:3.13|P-value:8.66E-4||SGD DESC:Translation elongation factor eIF-5A; previously thought to function in translation initiation; undergoes an essential hypusination modification; expressed under anaerobic conditions; ANB1 has a paralog, HYP2, that arose from the whole genome duplication Gene:ARO1(YDR127W)|FD-Score:-4.36|P-value:6.50E-6||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO7(YPR060C)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:CTF8(YHR191C)|FD-Score:-3.6|P-value:1.58E-4||SGD DESC:Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion Gene:FYV8(YGR196C)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GLY1(YEL046C)|FD-Score:4.86|P-value:6.00E-7||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:HAP4(YKL109W)|FD-Score:-3.19|P-value:7.20E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex Gene:HMG2(YLR450W)|FD-Score:-3.42|P-value:3.12E-4||SGD DESC:HMG-CoA reductase; converts HMG-CoA to mevalonate, a rate-limiting step in sterol biosynthesis; one of two isozymes; overproduction induces assembly of peripheral ER membrane arrays and short nuclear-associated membrane stacks; forms foci at the nuclear periphery upon DNA replication stress Gene:IKS1(YJL057C)|FD-Score:-3.22|P-value:6.36E-4||SGD DESC:Protein kinase of unknown cellular role; putative serine/threonine kinase; expression is induced during mild heat stress; deletion mutants are hypersensitive to copper sulphate and resistant to sorbate; interacts with an N-terminal fragment of Sst2p Gene:LAT1(YNL071W)|FD-Score:3.78|P-value:7.95E-5||SGD DESC:Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA Gene:LSM6(YDR378C)|FD-Score:-4.95|P-value:3.67E-7||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MAD1(YGL086W)|FD-Score:4.18|P-value:1.47E-5||SGD DESC:Coiled-coil protein involved in the spindle-assembly checkpoint; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon spindle assembly checkpoint arrest; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of the activity of the anaphase promoting complex; forms a complex with Mad2p Gene:MAD3(YJL013C)|FD-Score:-3.19|P-value:7.00E-4||SGD DESC:Subunit of the spindle-assembly checkpoint complex, which delays anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover Gene:MEF2(YJL102W)|FD-Score:-3.33|P-value:4.29E-4||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:PES4(YFR023W)|FD-Score:3.27|P-value:5.34E-4||SGD DESC:Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication Gene:PKH3(YDR466W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PSY1(YKL076C_d)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C Gene:RED1(YLR263W)|FD-Score:3.76|P-value:8.42E-5||SGD DESC:Protein component of the synaptonemal complex axial elements, involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; interacts with Hop1p, Mec3p and Ddc1p Gene:RNR3(YIL066C)|FD-Score:4.81|P-value:7.52E-7||SGD DESC:Minor isoform of the large subunit of ribonucleotide-diphosphate reductase; the RNR complex catalyzes rate-limiting step in dNTP synthesis, regulated by DNA replication and DNA damage checkpoint pathways via localization of small subunits Gene:RPN13(YLR421C)|FD-Score:3.74|P-value:9.17E-5||SGD DESC:Subunit of the 19S regulatory particle of the 26S proteasome lid; acts as a ubiquitin receptor for the proteasome; null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; protein abundance increases in response to DNA replication stress Gene:SAC1(YKL212W)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAS3(YBL052C)|FD-Score:3.21|P-value:6.53E-4||SGD DESC:Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal Gene:SCO2(YBR024W)|FD-Score:4.35|P-value:6.91E-6||SGD DESC:Protein anchored to mitochondrial inner membrane; may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p; SCO2 has a paralog, SCO1, that arose from the whole genome duplication Gene:SPI1(YER150W)|FD-Score:-3.23|P-value:6.26E-4||SGD DESC:GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p Gene:SSN8(YNL025C)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance Gene:SWA2(YDR320C)|FD-Score:-3.21|P-value:6.60E-4||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:SYH1(YPL105C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:SYS1(YJL004C)|FD-Score:-3.24|P-value:5.97E-4||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:VAM6(YDL077C)|FD-Score:-3.58|P-value:1.69E-4||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VMA4(YOR332W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress Gene:YAL042C-A(YAL042C-A_d)|FD-Score:-3.18|P-value:7.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ERV46/YAL042W; YAL042C-A is a non-essential gene Gene:YDR161W(YDR161W_p)|FD-Score:3.1|P-value:9.78E-4||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YFL040W(YFL040W_p)|FD-Score:4.61|P-value:2.04E-6||SGD DESC:Putative transporter, member of the sugar porter family; YFL040W is not an essential gene Gene:YGR228W(YGR228W_d)|FD-Score:4.41|P-value:5.10E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YHR202W(YHR202W_p)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization Gene:YLR312C(YLR312C_p)|FD-Score:-3.09|P-value:9.90E-4||SGD DESC:Putative protein of unknown function Gene:YMR031W-A(YMR031W-A_d)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Gene:YMR090W(YMR090W_p)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Putative protein of unknown function with similarity to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability Gene:YNL143C(YNL143C_p)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOL037C(YOL037C_d)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOL036W Gene:YPK1(YKL126W)|FD-Score:4.52|P-value:3.07E-6||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR397C4.681.40E-61.13AFG2ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs
YCL043C3.551.92E-40.36PDI1Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication
YLR005W3.197.13E-40.21SSL1Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p
YER006W2.980.001450.23NUG1GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus
YDL212W2.750.002960.03SHR3Endoplasmic reticulum packaging chaperone, required for incorporation of amino acid permeases into COPII coated vesicles for transport to the cell surface
YOR310C2.720.003270.12NOP58Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA
YLR186W2.590.004750.05EMG1Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity
YNL007C2.550.005440.03SIS1Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; not functionally redundant with Ydj1p due to due to substrate specificity; has similarity to bacterial DnaJ proteins; protein abundance increases in response to DNA replication stress
YDR361C2.520.005860.06BCP1Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport
YER048W-A2.460.006990.01ISD11Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis
YIL004C2.450.007200.01BET1Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins
YDR280W2.440.007420.03RRP45Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
YGL055W2.410.008060.00OLE1Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
YLR105C2.410.008080.01SEN2Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen2p contains the active site for tRNA 5' splice site cleavage and has similarity to Sen34p and to Archaeal tRNA splicing endonuclease
YPL020C2.400.008230.10ULP1Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; sequestered to the nucleolus under stress conditions

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YEL046C4.866.00E-7GLY1Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis
YIL066C4.817.52E-7RNR3Minor isoform of the large subunit of ribonucleotide-diphosphate reductase; the RNR complex catalyzes rate-limiting step in dNTP synthesis, regulated by DNA replication and DNA damage checkpoint pathways via localization of small subunits
YFL040W_p4.612.04E-6YFL040W_pPutative transporter, member of the sugar porter family; YFL040W is not an essential gene
YKL126W4.523.07E-6YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YGR228W_d4.415.10E-6YGR228W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C
YBR024W4.356.91E-6SCO2Protein anchored to mitochondrial inner membrane; may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p; SCO2 has a paralog, SCO1, that arose from the whole genome duplication
YGL086W4.181.47E-5MAD1Coiled-coil protein involved in the spindle-assembly checkpoint; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon spindle assembly checkpoint arrest; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of the activity of the anaphase promoting complex; forms a complex with Mad2p
YGL071W3.807.17E-5AFT1Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YNL071W3.787.95E-5LAT1Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA
YLR263W3.768.42E-5RED1Protein component of the synaptonemal complex axial elements, involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; interacts with Hop1p, Mec3p and Ddc1p
YLR421C3.749.17E-5RPN13Subunit of the 19S regulatory particle of the 26S proteasome lid; acts as a ubiquitin receptor for the proteasome; null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; protein abundance increases in response to DNA replication stress
YPL105C3.721.01E-4SYH1Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication
YMR031W-A_d3.701.08E-4YMR031W-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C
YHR202W_p3.581.71E-4YHR202W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization
YNL143C_p3.571.77E-4YNL143C_pProtein of unknown function; expressed at both mRNA and protein levels

GO enrichment analysis for SGTC_2895
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1292.16E-23SGTC_14113909-8223 150.0 μMChemDiv (Drug-like library)X14110.25
0.1031.93E-15SGTC_14504112-4260 37.6 μMChemDiv (Drug-like library)16331750.106667calcium & mitochondrial duress
0.0912.42E-12SGTC_7243910-0327 137.0 μMChemDiv (Drug-like library)28854830.112676ERG2
0.0881.45E-11SGTC_7014424-0219 185.0 μMChemDiv (Drug-like library)29117790.11236calcium & mitochondrial duress
0.0872.72E-11SGTC_1627st003713 25.6 μMTimTec (Natural product derivative library)161950700.113636calcium & mitochondrial duress
0.0814.31E-10SGTC_234nsc-17383 10.7 μMMiscellaneous4089740.138889ERG2
0.0807.82E-10SGTC_1464k208-0027 110.0 μMChemDiv (Drug-like library)X14640.309859
0.0791.04E-9SGTC_14023909-7673 35.9 μMChemDiv (Drug-like library)53280140.166667
0.0791.33E-9SGTC_1826st055655 41.6 μMTimTec (Natural product derivative library)6761680.157143ERG2
0.0781.78E-9SGTC_28899059656 52.0 μMChembridge (Drug-like library)39498380.15
0.0781.84E-9SGTC_10543429-0080 167.0 μMChemDiv (Drug-like library)11628770.202899
0.0782.08E-9SGTC_1974544-0043 41.6 μMChemDiv (Drug-like library)8965230.130435endomembrane recycling
0.0782.16E-9SGTC_1973st070275 50.2 μMTimTec (Natural product derivative library)171183340.1875
0.0773.00E-9SGTC_1071trifluoperazine 6.3 μMNIH Clinical Collection29135350.15493
0.0773.72E-9SGTC_1748st037455 53.6 μMTimTec (Natural product derivative library)5806900.161765

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_2838900268771.43 μM0.6666676457060Chembridge (Drug-like library)357.877063.64713fatty acid desaturase (OLE1)
SGTC_2993905425771.43 μM0.6444446470217Chembridge (Drug-like library)343.850483.16113fatty acid desaturase (OLE1)
SGTC_2893904848825.97 μM0.6170216467338Chembridge (Drug-like library)337.458583.46913fatty acid desaturase (OLE1)
SGTC_2994905352071.43 μM0.5957454647384Chembridge (Drug-like library)300.782683.27912RPP1 & pyrimidine depletion
SGTC_2887904478458.44 μM0.5744686465546Chembridge (Drug-like library)323.4322.98313fatty acid desaturase (OLE1)
SGTC_2880904298358.44 μM0.4545456464714Chembridge (Drug-like library)341.4224633.18914fatty acid desaturase (OLE1)
SGTC_3033909088749.47 μM0.4509819325384Chembridge (Drug-like library)342.862423.9402fatty acid desaturase (OLE1)
SGTC_1300136-0219337.66 μM0.428571234244ChemDiv (Drug-like library)296.148643.77812
SGTC_2822799471871.43 μM0.4237291018011Chembridge (Drug-like library)373.876463.58214
SGTC_2896905265671.43 μM0.4137936469436Chembridge (Drug-like library)369.887763.62913endomembrane recycling