Deletion Strain | FD score | P-value | Clearance | Gene | Gene Description |
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YMR290W-A_d | 7.20 | 3.06E-13 | 2.48 | YMR290W-A_d | Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase |
YKL210W | 4.71 | 1.21E-6 | 0.72 | UBA1 | Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress |
YNL267W | 4.00 | 3.20E-5 | 0.05 | PIK1 | Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton |
YLL031C | 3.95 | 3.93E-5 | 0.15 | GPI13 | ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein |
YMR001C | 3.80 | 7.38E-5 | 0.15 | CDC5 | Polo-like kinase with multiple functions in mitosis and cytokinesis through substrate phosphorylation, also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate |
YER146W | 3.65 | 1.32E-4 | 0.04 | LSM5 | Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA |
YGL099W | 3.60 | 1.57E-4 | 0.20 | LSG1 | Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm |
YDR527W | 3.40 | 3.32E-4 | 0.01 | RBA50 | Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 |
YAR019C | 3.40 | 3.41E-4 | 0.01 | CDC15 | Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress |
YHR062C | 3.39 | 3.49E-4 | 0.26 | RPP1 | Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs |
YBR109C | 3.13 | 8.78E-4 | 0.02 | CMD1 | Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin |
YIL061C | 3.11 | 9.45E-4 | 0.04 | SNP1 | Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress |
YPR055W | 3.07 | 0.00106 | 0.01 | SEC8 | Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress |
YBR140C | 3.06 | 0.00111 | 0.02 | IRA1 | GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication |
YML092C | 3.04 | 0.00119 | 0.14 | PRE8 | Alpha 2 subunit of the 20S proteasome |