7225135

N-tert-butyl-2-(2,4-dimethoxyphenyl)quinoline-4-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2904
Screen concentration 42.0 μM
Source Chembridge (Drug-like library)
PubChem CID 4446116
SMILES CC(C)(C)NC(=O)C1=CC(=NC2=CC=CC=C21)C3=C(C=C(C=C3)OC)OC
Standardized SMILES COc1ccc(c(OC)c1)c2cc(C(=O)NC(C)(C)C)c3ccccc3n2
Molecular weight 364.4376
ALogP 4.07
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 19.19
% growth inhibition (Hom. pool) 10.57


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4446116
Download HIP data (tab-delimited text)  (excel)
Gene:ARP2(YDL029W)|FD-Score:4.27|P-value:9.93E-6|Clearance:0.74||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:CDC3(YLR314C)|FD-Score:3.09|P-value:9.99E-4|Clearance:0.01||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:DML1(YMR211W)|FD-Score:3.32|P-value:4.55E-4|Clearance:0.05||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:INN1(YNL152W)|FD-Score:3.24|P-value:6.00E-4|Clearance:0.15||SGD DESC:Essential protein that associates with contractile actomyosin ring; required for ingression of the plasma membrane into the bud neck during cytokinesis; C2 domain, a membrane targeting module, is required for function; activates chitin synthase activity of Chs2p during cytokinesis Gene:NTR2(YKR022C)|FD-Score:3.43|P-value:3.00E-4|Clearance:0.08||SGD DESC:Essential protein that forms a dimer with Ntr1p; also forms a trimer, with Ntr2p and the DExD/H-box RNA helicase Prp43p, that is involved in spliceosome disassembly Gene:PRI2(YKL045W)|FD-Score:3.53|P-value:2.11E-4|Clearance:0.09||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:SED5(YLR026C)|FD-Score:3.35|P-value:4.02E-4|Clearance:0.03||SGD DESC:cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins Gene:TCP1(YDR212W)|FD-Score:-3.29|P-value:5.01E-4|Clearance:0||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:TEM1(YML064C)|FD-Score:3.32|P-value:4.49E-4|Clearance:0||SGD DESC:GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis Gene:YTM1(YOR272W)|FD-Score:3.27|P-value:5.45E-4|Clearance:0.03||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats Gene:ARP2(YDL029W)|FD-Score:4.27|P-value:9.93E-6|Clearance:0.74||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:CDC3(YLR314C)|FD-Score:3.09|P-value:9.99E-4|Clearance:0.01||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:DML1(YMR211W)|FD-Score:3.32|P-value:4.55E-4|Clearance:0.05||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:INN1(YNL152W)|FD-Score:3.24|P-value:6.00E-4|Clearance:0.15||SGD DESC:Essential protein that associates with contractile actomyosin ring; required for ingression of the plasma membrane into the bud neck during cytokinesis; C2 domain, a membrane targeting module, is required for function; activates chitin synthase activity of Chs2p during cytokinesis Gene:NTR2(YKR022C)|FD-Score:3.43|P-value:3.00E-4|Clearance:0.08||SGD DESC:Essential protein that forms a dimer with Ntr1p; also forms a trimer, with Ntr2p and the DExD/H-box RNA helicase Prp43p, that is involved in spliceosome disassembly Gene:PRI2(YKL045W)|FD-Score:3.53|P-value:2.11E-4|Clearance:0.09||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:SED5(YLR026C)|FD-Score:3.35|P-value:4.02E-4|Clearance:0.03||SGD DESC:cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins Gene:TCP1(YDR212W)|FD-Score:-3.29|P-value:5.01E-4|Clearance:0||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:TEM1(YML064C)|FD-Score:3.32|P-value:4.49E-4|Clearance:0||SGD DESC:GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis Gene:YTM1(YOR272W)|FD-Score:3.27|P-value:5.45E-4|Clearance:0.03||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4446116
Download HOP data (tab-delimited text)  (excel)
Gene:AFR1(YDR085C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Protein required for pheromone-induced projection (shmoo) formation; regulates septin architecture during mating; has an RVXF motif that mediates targeting of Glc7p to mating projections; interacts with Cdc12p; AFR1 has a paralog, YER158C, that arose from the whole genome duplication Gene:CDC50(YCR094W)|FD-Score:-5.17|P-value:1.20E-7||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CKA1(YIL035C)|FD-Score:4.09|P-value:2.16E-5||SGD DESC:Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching Gene:FYV6(YNL133C)|FD-Score:-5.31|P-value:5.50E-8||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:HUA2(YOR284W)|FD-Score:3.91|P-value:4.53E-5||SGD DESC:Cytoplasmic protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:ISY1(YJR050W)|FD-Score:4.38|P-value:5.81E-6||SGD DESC:Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles Gene:KDX1(YKL161C)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p Gene:MGA1(YGR249W)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Protein similar to heat shock transcription factor; multicopy suppressor of pseudohyphal growth defects of ammonium permease mutants Gene:MRPL51(YPR100W)|FD-Score:-3.66|P-value:1.29E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NGG1(YDR176W)|FD-Score:-3.31|P-value:4.62E-4||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:OAF3(YKR064W)|FD-Score:3.2|P-value:6.77E-4||SGD DESC:Putative transcriptional repressor with Zn(2)-Cys(6) finger; negatively regulates transcription in response to oleate levels, based on mutant phenotype and localization to oleate-responsive promoters; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:PDR1(YGL013C)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PTH2(YBL057C)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:RFM1(YOR279C)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RPS16A(YMR143W)|FD-Score:-3.25|P-value:5.81E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication Gene:RRT1(YBL048W_d)|FD-Score:3.91|P-value:4.67E-5||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:SAC6(YDR129C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SIP3(YNL257C)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication Gene:SWA2(YDR320C)|FD-Score:-3.92|P-value:4.42E-5||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:TDA7(YNL176C_p)|FD-Score:8.15|P-value:1.85E-16||SGD DESC:Cell cycle-regulated gene of unknown function, promoter bound by Fkh2p; null mutant is sensitive to expression of the top1-T722A allele Gene:UBA3(YPR066W)|FD-Score:3.12|P-value:9.17E-4||SGD DESC:Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:VMA16(YHR026W)|FD-Score:-3.43|P-value:3.07E-4||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:YBR196C-A(YBR196C-A_p)|FD-Score:-3.28|P-value:5.23E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDL073W(YDL073W_p)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Putative protein of unknown function; YDL073W is not an essential gene Gene:YIP5(YGL161C)|FD-Score:-3.78|P-value:7.74E-5||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YIR020W-A(YIR020W-A_d)|FD-Score:3.25|P-value:5.86E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YJL215C(YJL215C_d)|FD-Score:5.44|P-value:2.70E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR296W(YLR296W_d)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR366W(YLR366W_d)|FD-Score:5.14|P-value:1.35E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A Gene:YML003W(YML003W_p)|FD-Score:3.87|P-value:5.47E-5||SGD DESC:Putative protein of unknown function Gene:YMR082C(YMR082C_d)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL168W(YPL168W_p)|FD-Score:3.12|P-value:8.94E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated Gene:YUR1(YJL139C)|FD-Score:3.25|P-value:5.87E-4||SGD DESC:Mannosyltransferase involved in protein N-glycosylation; member of the KTR1 family; located in the Golgi apparatus; YUR1 has a paralog, KTR2, that arose from the whole genome duplication Gene:AFR1(YDR085C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Protein required for pheromone-induced projection (shmoo) formation; regulates septin architecture during mating; has an RVXF motif that mediates targeting of Glc7p to mating projections; interacts with Cdc12p; AFR1 has a paralog, YER158C, that arose from the whole genome duplication Gene:CDC50(YCR094W)|FD-Score:-5.17|P-value:1.20E-7||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CKA1(YIL035C)|FD-Score:4.09|P-value:2.16E-5||SGD DESC:Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching Gene:FYV6(YNL133C)|FD-Score:-5.31|P-value:5.50E-8||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:HUA2(YOR284W)|FD-Score:3.91|P-value:4.53E-5||SGD DESC:Cytoplasmic protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:ISY1(YJR050W)|FD-Score:4.38|P-value:5.81E-6||SGD DESC:Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles Gene:KDX1(YKL161C)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p Gene:MGA1(YGR249W)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Protein similar to heat shock transcription factor; multicopy suppressor of pseudohyphal growth defects of ammonium permease mutants Gene:MRPL51(YPR100W)|FD-Score:-3.66|P-value:1.29E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NGG1(YDR176W)|FD-Score:-3.31|P-value:4.62E-4||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:OAF3(YKR064W)|FD-Score:3.2|P-value:6.77E-4||SGD DESC:Putative transcriptional repressor with Zn(2)-Cys(6) finger; negatively regulates transcription in response to oleate levels, based on mutant phenotype and localization to oleate-responsive promoters; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:PDR1(YGL013C)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PTH2(YBL057C)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:RFM1(YOR279C)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RPS16A(YMR143W)|FD-Score:-3.25|P-value:5.81E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication Gene:RRT1(YBL048W_d)|FD-Score:3.91|P-value:4.67E-5||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:SAC6(YDR129C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SIP3(YNL257C)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication Gene:SWA2(YDR320C)|FD-Score:-3.92|P-value:4.42E-5||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:TDA7(YNL176C_p)|FD-Score:8.15|P-value:1.85E-16||SGD DESC:Cell cycle-regulated gene of unknown function, promoter bound by Fkh2p; null mutant is sensitive to expression of the top1-T722A allele Gene:UBA3(YPR066W)|FD-Score:3.12|P-value:9.17E-4||SGD DESC:Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:VMA16(YHR026W)|FD-Score:-3.43|P-value:3.07E-4||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:YBR196C-A(YBR196C-A_p)|FD-Score:-3.28|P-value:5.23E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDL073W(YDL073W_p)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Putative protein of unknown function; YDL073W is not an essential gene Gene:YIP5(YGL161C)|FD-Score:-3.78|P-value:7.74E-5||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YIR020W-A(YIR020W-A_d)|FD-Score:3.25|P-value:5.86E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YJL215C(YJL215C_d)|FD-Score:5.44|P-value:2.70E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR296W(YLR296W_d)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR366W(YLR366W_d)|FD-Score:5.14|P-value:1.35E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A Gene:YML003W(YML003W_p)|FD-Score:3.87|P-value:5.47E-5||SGD DESC:Putative protein of unknown function Gene:YMR082C(YMR082C_d)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL168W(YPL168W_p)|FD-Score:3.12|P-value:8.94E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated Gene:YUR1(YJL139C)|FD-Score:3.25|P-value:5.87E-4||SGD DESC:Mannosyltransferase involved in protein N-glycosylation; member of the KTR1 family; located in the Golgi apparatus; YUR1 has a paralog, KTR2, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL029W4.279.93E-60.74ARP2Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity
YKL045W3.532.11E-40.09PRI2Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
YKR022C3.433.00E-40.08NTR2Essential protein that forms a dimer with Ntr1p; also forms a trimer, with Ntr2p and the DExD/H-box RNA helicase Prp43p, that is involved in spliceosome disassembly
YLR026C3.354.02E-40.03SED5cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins
YML064C3.324.49E-40.00TEM1GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis
YMR211W3.324.55E-40.05DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
YOR272W3.275.45E-40.03YTM1Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats
YNL152W3.246.00E-40.15INN1Essential protein that associates with contractile actomyosin ring; required for ingression of the plasma membrane into the bud neck during cytokinesis; C2 domain, a membrane targeting module, is required for function; activates chitin synthase activity of Chs2p during cytokinesis
YLR314C3.099.99E-40.01CDC3Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YDL045C3.080.001050.02FAD1Flavin adenine dinucleotide (FAD) synthetase, performs the second step in synthesis of FAD from riboflavin
YIL031W3.050.001130.00ULP2Peptidase that deconjugates Smt3/SUMO-1 peptides from proteins, plays a role in chromosome cohesion at centromeric regions and recovery from checkpoint arrest induced by DNA damage or DNA replication defects; potential Cdc28p substrate
YFL039C3.050.001130.03ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YCL041C_d3.020.001250.05YCL041C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps both the verified gene PDI1/YCL043C and the uncharacterized gene YCL042W
YGL123W2.980.001460.07RPS2Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5
YLR438C-A2.910.001810.02LSM3Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNL176C_p8.151.85E-16TDA7_pCell cycle-regulated gene of unknown function, promoter bound by Fkh2p; null mutant is sensitive to expression of the top1-T722A allele
YJL215C_d5.442.70E-8YJL215C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YLR366W_d5.141.35E-7YLR366W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A
YJR050W4.385.81E-6ISY1Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles
YIL035C4.092.16E-5CKA1Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching
YOR284W3.914.53E-5HUA2Cytoplasmic protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly
YBL048W_d3.914.67E-5RRT1_dIdentified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data
YML003W_p3.875.47E-5YML003W_pPutative protein of unknown function
YDR129C3.701.07E-4SAC6Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress
YGL013C3.621.46E-4PDR1Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
YMR082C_d3.621.47E-4YMR082C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YDR085C3.611.51E-4AFR1Protein required for pheromone-induced projection (shmoo) formation; regulates septin architecture during mating; has an RVXF motif that mediates targeting of Glc7p to mating projections; interacts with Cdc12p; AFR1 has a paralog, YER158C, that arose from the whole genome duplication
YDL073W_p3.601.58E-4YDL073W_pPutative protein of unknown function; YDL073W is not an essential gene
YKL161C3.354.01E-4KDX1Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p
YNL257C3.324.57E-4SIP3Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication

GO enrichment analysis for SGTC_2904
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1318.42E-24SGTC_60844-0013 19.8 μMChemDiv (Drug-like library)15849000.106667
0.1292.81E-23SGTC_249418-aminoabieta-8,11,13-triene sulfate 3.7 μMMicrosource (Natural product library)167250760.0595238
0.1288.27E-23SGTC_30009075062 71.4 μMChembridge (Drug-like library)170578710.101266
0.1102.50E-17SGTC_12430416-0003 5.7 μMChemDiv (Drug-like library)21419770.0434783PDR1
0.1064.59E-16SGTC_29015-(ethoxymethyl)quinolin-8-ol 39.7 μMChembridge (Drug-like library)2796210.126761
0.0791.06E-9SGTC_295k015-0027 31.0 μMChemDiv (Drug-like library)38874240.0886076PDR1
0.0781.94E-9SGTC_1700st030840 7.9 μMTimTec (Natural product derivative library)54993070.155556PDR1
0.0764.47E-9SGTC_2023594-0513 34.8 μMChemDiv (Drug-like library)7084290.136364PDR1
0.0732.07E-8SGTC_23579067248 200.0 μMChembridge (Fragment library)91502350.0684932
0.0732.41E-8SGTC_8041595-0054 20.9 μMChemDiv (Drug-like library)7615230.123288sphingolipid biosynthesis & PDR1
0.0714.98E-8SGTC_683panaxydol 45.0 μMMiscellaneous52832800.0119048sphingolipid biosynthesis & PDR1
0.0691.20E-7SGTC_32589138477 49.5 μMChembridge (Drug-like library)49703550.186047
0.0672.54E-7SGTC_3823229-0966 23.2 μMChemDiv (Drug-like library)57223700.146667
0.0656.12E-7SGTC_31639099914 49.5 μMChembridge (Drug-like library)162683680.191781
0.0656.41E-7SGTC_250brefeldin a 552.2 μMICCB bioactive library64361870.047619PDR1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2863902873758.44 μM0.43756460917Chembridge (Drug-like library)322.357823.0514
SGTC_3170910305449.47 μM0.36363616432970Chembridge (Drug-like library)343.3537633.8021460S ribosome export
SGTC_1914st06236772.6 μM0.359375978205TimTec (Natural product derivative library)275.299781.98214
SGTC_12900986-024910.2 μM0.32758653329ChemDiv (Drug-like library)300.353844.88613DNA intercalators
SGTC_558k064-002721.3 μM0.317465088526ChemDiv (Drug-like library)334.79895.55113DNA damage response
SGTC_1867st05737264 μM0.306452688837TimTec (Natural product derivative library)312.316643.08705
SGTC_11413253-038952.6 μM0.3043485402031ChemDiv (Drug-like library)290.337562.60315
SGTC_1866st05763864 μM0.301587688802TimTec (Natural product derivative library)312.316643.08705
SGTC_3195911308249.47 μM0.30158717018038Chembridge (Drug-like library)301.337063.05114
SGTC_1615st00265686.5 μM0.2923081584458TimTec (Natural product derivative library)231.253821.1424redox potentiating