7799065

1-(4-bromo-2-chlorophenyl)-3-(4-chloro-2,5-dimethoxyphenyl)urea

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2905
Screen concentration 20.1 μM
Source Chembridge (Drug-like library)
PubChem CID 1296806
SMILES COC1=CC(=C(C=C1NC(=O)NC2=C(C=C(C=C2)Br)Cl)OC)Cl
Standardized SMILES COc1cc(NC(=O)Nc2ccc(Br)cc2Cl)c(OC)cc1Cl
Molecular weight 420.0853
ALogP 4.57
H-bond donor count 2
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 15.57
% growth inhibition (Hom. pool) 10.55


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1296806
Download HIP data (tab-delimited text)  (excel)
Gene:ACS2(YLR153C)|FD-Score:-3.8|P-value:7.26E-5|Clearance:0||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions Gene:AME1(YBR211C)|FD-Score:3.29|P-value:5.00E-4|Clearance:0.01||SGD DESC:Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress Gene:ARC19(YKL013C)|FD-Score:-3.2|P-value:6.90E-4|Clearance:0||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARC35(YNR035C)|FD-Score:-4.67|P-value:1.52E-6|Clearance:0||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin Gene:BET4(YJL031C)|FD-Score:6.65|P-value:1.51E-11|Clearance:0.88||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:BOS1(YLR078C)|FD-Score:4.3|P-value:8.54E-6|Clearance:0.41||SGD DESC:v-SNARE (vesicle specific SNAP receptor), localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi Gene:CDC15(YAR019C)|FD-Score:-4.74|P-value:1.08E-6|Clearance:0||SGD DESC:Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress Gene:CDC37(YDR168W)|FD-Score:3.69|P-value:1.13E-4|Clearance:0.03||SGD DESC:Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding Gene:CDC6(YJL194W)|FD-Score:-3.65|P-value:1.30E-4|Clearance:0||SGD DESC:Essential ATP-binding protein required for DNA replication; component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; homologous to S. pombe Cdc18p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:CDS1(YBR029C)|FD-Score:-9.62|P-value:3.37E-22|Clearance:0||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:CNS1(YBR155W)|FD-Score:-3.75|P-value:8.92E-5|Clearance:0||SGD DESC:TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion Gene:ECM9(YKR004C)|FD-Score:3.26|P-value:5.56E-4|Clearance:0.01||SGD DESC:Non-essential protein of unknown function Gene:EFB1(YAL003W)|FD-Score:-5.27|P-value:6.85E-8|Clearance:0||SGD DESC:Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site Gene:FAS1(YKL182W)|FD-Score:3.66|P-value:1.26E-4|Clearance:0.19||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:GLC7(YER133W)|FD-Score:-4.55|P-value:2.66E-6|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GPI14(YJR013W)|FD-Score:3.89|P-value:4.94E-5|Clearance:0.21||SGD DESC:Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M Gene:HSH49(YOR319W)|FD-Score:-7.15|P-value:4.31E-13|Clearance:0||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:IRR1(YIL026C)|FD-Score:-3.76|P-value:8.43E-5|Clearance:0||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:LSM3(YLR438C-A)|FD-Score:3.39|P-value:3.46E-4|Clearance:0.1||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:LSM4(YER112W)|FD-Score:5.16|P-value:1.23E-7|Clearance:0.39||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:NUP49(YGL172W)|FD-Score:6.04|P-value:7.81E-10|Clearance:0.88||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:NUS1(YDL193W)|FD-Score:-5.23|P-value:8.32E-8|Clearance:0||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PIK1(YNL267W)|FD-Score:-3.18|P-value:7.45E-4|Clearance:0||SGD DESC:Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton Gene:PRE3(YJL001W)|FD-Score:-5.3|P-value:5.91E-8|Clearance:0||SGD DESC:Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides Gene:PRE8(YML092C)|FD-Score:-4.66|P-value:1.57E-6|Clearance:0||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:PRI1(YIR008C)|FD-Score:3.25|P-value:5.68E-4|Clearance:0.03||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:PRP24(YMR268C)|FD-Score:-4.27|P-value:9.60E-6|Clearance:0||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:PRP45(YAL032C)|FD-Score:-9.92|P-value:1.76E-23|Clearance:0||SGD DESC:Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene Gene:PRP6(YBR055C)|FD-Score:3.28|P-value:5.21E-4|Clearance:0.02||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:REB1(YBR049C)|FD-Score:-3.57|P-value:1.78E-4|Clearance:0||SGD DESC:RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication Gene:RER2(YBR002C)|FD-Score:-3.39|P-value:3.48E-4|Clearance:0||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RNR2(YJL026W)|FD-Score:-3.79|P-value:7.63E-5|Clearance:0||SGD DESC:Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits Gene:RPL15A(YLR029C)|FD-Score:4.77|P-value:9.22E-7|Clearance:0.06||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RRN7(YJL025W)|FD-Score:-4.49|P-value:3.57E-6|Clearance:0||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:SEC4(YFL005W)|FD-Score:3.22|P-value:6.33E-4|Clearance:0.03||SGD DESC:Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane Gene:SLY1(YDR189W)|FD-Score:4.71|P-value:1.23E-6|Clearance:0.41||SGD DESC:Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex Gene:SPT16(YGL207W)|FD-Score:-5.84|P-value:2.63E-9|Clearance:0||SGD DESC:Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome Gene:SRP72(YPL210C)|FD-Score:3.47|P-value:2.57E-4|Clearance:0.08||SGD DESC:Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane Gene:SSC1(YJR045C)|FD-Score:-3.32|P-value:4.52E-4|Clearance:0||SGD DESC:Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication Gene:TFC3(YAL001C)|FD-Score:-6.71|P-value:9.95E-12|Clearance:0||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TIM23(YNR017W)|FD-Score:-3.47|P-value:2.56E-4|Clearance:0||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel Gene:TRM112(YNR046W)|FD-Score:-3.98|P-value:3.50E-5|Clearance:0||SGD DESC:Protein involved in methylation of tRNA, rRNA, and translation factors; subunit of tRNA methyltransferase (MTase) complexes in combination with Trm9p and Trm11p; interacts with and stabilizes 18S rRNA methyltransferase Bud23p; subunit of complex with Mtq2p that methylates Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; deletion confers resistance to zymocin; relative distribution to the nucleus increases upon DNA replication stress Gene:TUB1(YML085C)|FD-Score:3.2|P-value:6.98E-4|Clearance:0.14||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:YAL034C-B(YAL034C-B_d)|FD-Score:-3.83|P-value:6.33E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL196W(YDL196W_d)|FD-Score:-3.21|P-value:6.71E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YNL114C(YNL114C_d)|FD-Score:3.26|P-value:5.54E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Gene:YOR102W(YOR102W_d)|FD-Score:-3.12|P-value:8.94E-4|Clearance:0||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex Gene:ACS2(YLR153C)|FD-Score:-3.8|P-value:7.26E-5|Clearance:0||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions Gene:AME1(YBR211C)|FD-Score:3.29|P-value:5.00E-4|Clearance:0.01||SGD DESC:Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress Gene:ARC19(YKL013C)|FD-Score:-3.2|P-value:6.90E-4|Clearance:0||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARC35(YNR035C)|FD-Score:-4.67|P-value:1.52E-6|Clearance:0||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin Gene:BET4(YJL031C)|FD-Score:6.65|P-value:1.51E-11|Clearance:0.88||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:BOS1(YLR078C)|FD-Score:4.3|P-value:8.54E-6|Clearance:0.41||SGD DESC:v-SNARE (vesicle specific SNAP receptor), localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi Gene:CDC15(YAR019C)|FD-Score:-4.74|P-value:1.08E-6|Clearance:0||SGD DESC:Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress Gene:CDC37(YDR168W)|FD-Score:3.69|P-value:1.13E-4|Clearance:0.03||SGD DESC:Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding Gene:CDC6(YJL194W)|FD-Score:-3.65|P-value:1.30E-4|Clearance:0||SGD DESC:Essential ATP-binding protein required for DNA replication; component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; homologous to S. pombe Cdc18p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:CDS1(YBR029C)|FD-Score:-9.62|P-value:3.37E-22|Clearance:0||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:CNS1(YBR155W)|FD-Score:-3.75|P-value:8.92E-5|Clearance:0||SGD DESC:TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion Gene:ECM9(YKR004C)|FD-Score:3.26|P-value:5.56E-4|Clearance:0.01||SGD DESC:Non-essential protein of unknown function Gene:EFB1(YAL003W)|FD-Score:-5.27|P-value:6.85E-8|Clearance:0||SGD DESC:Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site Gene:FAS1(YKL182W)|FD-Score:3.66|P-value:1.26E-4|Clearance:0.19||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:GLC7(YER133W)|FD-Score:-4.55|P-value:2.66E-6|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GPI14(YJR013W)|FD-Score:3.89|P-value:4.94E-5|Clearance:0.21||SGD DESC:Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M Gene:HSH49(YOR319W)|FD-Score:-7.15|P-value:4.31E-13|Clearance:0||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:IRR1(YIL026C)|FD-Score:-3.76|P-value:8.43E-5|Clearance:0||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:LSM3(YLR438C-A)|FD-Score:3.39|P-value:3.46E-4|Clearance:0.1||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:LSM4(YER112W)|FD-Score:5.16|P-value:1.23E-7|Clearance:0.39||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:NUP49(YGL172W)|FD-Score:6.04|P-value:7.81E-10|Clearance:0.88||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:NUS1(YDL193W)|FD-Score:-5.23|P-value:8.32E-8|Clearance:0||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PIK1(YNL267W)|FD-Score:-3.18|P-value:7.45E-4|Clearance:0||SGD DESC:Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton Gene:PRE3(YJL001W)|FD-Score:-5.3|P-value:5.91E-8|Clearance:0||SGD DESC:Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides Gene:PRE8(YML092C)|FD-Score:-4.66|P-value:1.57E-6|Clearance:0||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:PRI1(YIR008C)|FD-Score:3.25|P-value:5.68E-4|Clearance:0.03||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:PRP24(YMR268C)|FD-Score:-4.27|P-value:9.60E-6|Clearance:0||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:PRP45(YAL032C)|FD-Score:-9.92|P-value:1.76E-23|Clearance:0||SGD DESC:Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene Gene:PRP6(YBR055C)|FD-Score:3.28|P-value:5.21E-4|Clearance:0.02||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:REB1(YBR049C)|FD-Score:-3.57|P-value:1.78E-4|Clearance:0||SGD DESC:RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication Gene:RER2(YBR002C)|FD-Score:-3.39|P-value:3.48E-4|Clearance:0||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RNR2(YJL026W)|FD-Score:-3.79|P-value:7.63E-5|Clearance:0||SGD DESC:Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits Gene:RPL15A(YLR029C)|FD-Score:4.77|P-value:9.22E-7|Clearance:0.06||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RRN7(YJL025W)|FD-Score:-4.49|P-value:3.57E-6|Clearance:0||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:SEC4(YFL005W)|FD-Score:3.22|P-value:6.33E-4|Clearance:0.03||SGD DESC:Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane Gene:SLY1(YDR189W)|FD-Score:4.71|P-value:1.23E-6|Clearance:0.41||SGD DESC:Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex Gene:SPT16(YGL207W)|FD-Score:-5.84|P-value:2.63E-9|Clearance:0||SGD DESC:Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome Gene:SRP72(YPL210C)|FD-Score:3.47|P-value:2.57E-4|Clearance:0.08||SGD DESC:Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane Gene:SSC1(YJR045C)|FD-Score:-3.32|P-value:4.52E-4|Clearance:0||SGD DESC:Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication Gene:TFC3(YAL001C)|FD-Score:-6.71|P-value:9.95E-12|Clearance:0||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TIM23(YNR017W)|FD-Score:-3.47|P-value:2.56E-4|Clearance:0||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel Gene:TRM112(YNR046W)|FD-Score:-3.98|P-value:3.50E-5|Clearance:0||SGD DESC:Protein involved in methylation of tRNA, rRNA, and translation factors; subunit of tRNA methyltransferase (MTase) complexes in combination with Trm9p and Trm11p; interacts with and stabilizes 18S rRNA methyltransferase Bud23p; subunit of complex with Mtq2p that methylates Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; deletion confers resistance to zymocin; relative distribution to the nucleus increases upon DNA replication stress Gene:TUB1(YML085C)|FD-Score:3.2|P-value:6.98E-4|Clearance:0.14||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:YAL034C-B(YAL034C-B_d)|FD-Score:-3.83|P-value:6.33E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL196W(YDL196W_d)|FD-Score:-3.21|P-value:6.71E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YNL114C(YNL114C_d)|FD-Score:3.26|P-value:5.54E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Gene:YOR102W(YOR102W_d)|FD-Score:-3.12|P-value:8.94E-4|Clearance:0||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1296806
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:5.2|P-value:9.76E-8||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:AEP1(YMR064W)|FD-Score:-3.11|P-value:9.32E-4||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:AIM22(YJL046W)|FD-Score:-4.35|P-value:6.79E-6||SGD DESC:Putative lipoate-protein ligase, required along with Lip2 and Lip5 for lipoylation of Lat1p and Kgd2p; similar to E. coli LplA; null mutant displays reduced frequency of mitochondrial genome loss Gene:ASN1(YPR145W)|FD-Score:4.98|P-value:3.18E-7||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication Gene:BIK1(YCL029C)|FD-Score:-3.1|P-value:9.53E-4||SGD DESC:Microtubule-associated protein, component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170 Gene:BRE4(YDL231C)|FD-Score:4.45|P-value:4.25E-6||SGD DESC:Zinc finger protein containing five transmembrane domains; null mutant exhibits strongly fragmented vacuoles and sensitivity to brefeldin A, a drug which is known to affect intracellular transport Gene:BUD25(YER014C-A)|FD-Score:-3.91|P-value:4.71E-5||SGD DESC:Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern Gene:CAF4(YKR036C)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:WD40 repeat-containing protein associated with the CCR4-NOT complex; interacts in a Ccr4p-dependent manner with Ssn2p; also interacts with Fis1p, Mdv1p and Dnm1p and plays a role in mitochondrial fission; CAF4 has a paralog, MDV1, that arose from the whole genome duplication Gene:CAF40(YNL288W)|FD-Score:-3.35|P-value:4.07E-4||SGD DESC:Evolutionarily conserved subunit of the CCR4-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p Gene:CBT1(YKL208W)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Protein involved in 5' end processing of mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p Gene:COQ1(YBR003W)|FD-Score:-3.28|P-value:5.24E-4||SGD DESC:Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis Gene:COS111(YBR203W)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:COX10(YPL172C)|FD-Score:-4.01|P-value:3.02E-5||SGD DESC:Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders Gene:COX18(YGR062C)|FD-Score:-3.61|P-value:1.56E-4||SGD DESC:Mitochondrial integral inner membrane protein required for membrane insertion of C-terminus of Cox2p; interacts genetically and physically with Mss2p and Pnt1p; similar to S. cerevisiae Oxa1, N. crassa Oxa2p, and E. coli YidC Gene:DMC1(YER179W)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Meiosis-specific protein required for repair of double-strand breaks and pairing between homologous chromosomes; homolog of Rad51p and the bacterial RecA protein; binds ssDNA and dsDNA, forms helical filaments; stimulated by Rdh54p Gene:DPH1(YIL103W)|FD-Score:-3.49|P-value:2.44E-4||SGD DESC:Protein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p Gene:ECM33(YBR078W)|FD-Score:-8.54|P-value:6.81E-18||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:ELP3(YPL086C)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin Gene:FAU1(YER183C)|FD-Score:4|P-value:3.12E-5||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:GAL80(YML051W)|FD-Score:-4.25|P-value:1.07E-5||SGD DESC:Transcriptional regulator involved in the repression of GAL genes in the absence of galactose; inhibits transcriptional activation by Gal4p; inhibition relieved by Gal3p or Gal1p binding Gene:GCG1(YER163C_p)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle Gene:GDA1(YEL042W)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate Gene:GEP3(YOR205C)|FD-Score:-3.14|P-value:8.43E-4||SGD DESC:Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (phb1) Gene:GIR2(YDR152W)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Highly-acidic cytoplasmic RWD domain-containing protein of unknown function; forms a complex with Rbg2p; interacts with Rbg1p and Gcn1p; associates with translating ribosomes; putative intrinsically unstructured protein Gene:GLG1(YKR058W)|FD-Score:3.15|P-value:8.15E-4||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG1 has a paralog, GLG2, that arose from the whole genome duplication Gene:GTF1(YGR102C)|FD-Score:-3.78|P-value:7.96E-5||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; transposon insertion mutant is salt sensitive and null mutant has growth defects; non-tagged protein is detected in purified mitochondria Gene:GUP1(YGL084C)|FD-Score:-3.65|P-value:1.32E-4||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HAL9(YOL089C)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication Gene:IOC4(YMR044W)|FD-Score:4.02|P-value:2.93E-5||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing Gene:ISM1(YPL040C)|FD-Score:-3.54|P-value:2.00E-4||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:IZH2(YOL002C)|FD-Score:3.13|P-value:8.71E-4||SGD DESC:Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc Gene:MAC1(YMR021C)|FD-Score:-3.2|P-value:6.80E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MAL31(YBR298C)|FD-Score:-3.44|P-value:2.95E-4||SGD DESC:Maltose permease, high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL3 complex locus; member of the 12 transmembrane domain superfamily of sugar transporters; functional in genomic reference strain S288C Gene:MST1(YKL194C)|FD-Score:-4.07|P-value:2.31E-5||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:NGG1(YDR176W)|FD-Score:9.11|P-value:4.01E-20||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:NIT2(YJL126W)|FD-Score:4.1|P-value:2.11E-5||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:NUP42(YDR192C)|FD-Score:4.72|P-value:1.18E-6||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; interacts with Gle1p Gene:OAR1(YKL055C)|FD-Score:-3.25|P-value:5.84E-4||SGD DESC:Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p Gene:PMT2(YAL023C)|FD-Score:-4.76|P-value:9.54E-7||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication Gene:RAD17(YOR368W)|FD-Score:3.12|P-value:9.18E-4||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins Gene:RDI1(YDL135C)|FD-Score:5.57|P-value:1.31E-8||SGD DESC:Rho GDP dissociation inhibitor; involved in the localization and regulation of Cdc42p and Rho1p; protein abundance increases in response to DNA replication stress Gene:RNQ1(YCL028W)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:[PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate Gene:RPA49(YNL248C)|FD-Score:-3.79|P-value:7.42E-5||SGD DESC:RNA polymerase I subunit A49 Gene:RPL12A(YEL054C)|FD-Score:-3.12|P-value:9.17E-4||SGD DESC:Ribosomal 60S subunit protein L12A; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12A has a paralog, RPL12B, that arose from the whole genome duplication Gene:RPL22A(YLR061W)|FD-Score:-3.28|P-value:5.19E-4||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RSM22(YKL155C)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase Gene:RTC6(YPL183W-A)|FD-Score:-3.16|P-value:7.90E-4||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:SAC6(YDR129C)|FD-Score:-3.77|P-value:8.01E-5||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SDC1(YDR469W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 Gene:SLA1(YBL007C)|FD-Score:-3.58|P-value:1.74E-4||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SNF12(YNR023W)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive Gene:SNT309(YPR101W)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SOD1(YJR104C)|FD-Score:-3.29|P-value:4.98E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SSF1(YHR066W)|FD-Score:-3.54|P-value:2.04E-4||SGD DESC:Constituent of 66S pre-ribosomal particles; required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family; SSF1 has a paralog, SSF2, that arose from the whole genome duplication Gene:SSK2(YNR031C)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress Gene:TDA7(YNL176C_p)|FD-Score:5.22|P-value:8.90E-8||SGD DESC:Cell cycle-regulated gene of unknown function, promoter bound by Fkh2p; null mutant is sensitive to expression of the top1-T722A allele Gene:TIM8(YJR135W-A)|FD-Score:3.1|P-value:9.64E-4||SGD DESC:Mitochondrial intermembrane space protein, forms a complex with Tim13p that delivers a subset of hydrophobic proteins to the TIM22 complex for inner membrane insertion; homolog of human TIMM8A, implicated in Mohr-Tranebjaerg syndrome Gene:TMA16(YOR252W)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Protein of unknown function that associates with ribosomes Gene:TRI1(YMR233W)|FD-Score:4.46|P-value:4.06E-6||SGD DESC:Non-essential sumoylated protein of unknown function; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:3.73|P-value:9.75E-5||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VMA1(YDL185W)|FD-Score:-3.14|P-value:8.42E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VPS25(YJR102C)|FD-Score:-6.03|P-value:8.34E-10||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:YAL016C-B(YAL016C-B_d)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR154C(YDR154C_d)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein Gene:YEN1(YER041W)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p Gene:YER077C(YER077C_p)|FD-Score:-5.32|P-value:5.25E-8||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport Gene:YFL019C(YFL019C_d)|FD-Score:4.8|P-value:7.99E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Gene:YFR020W(YFR020W_p)|FD-Score:4.42|P-value:4.92E-6||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YML009W-B(YML009W-B_d)|FD-Score:-3.24|P-value:5.91E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Gene:YML122C(YML122C_d)|FD-Score:-4.82|P-value:7.15E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR318C(YOR318C_d)|FD-Score:3.86|P-value:5.67E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo Gene:YPL168W(YPL168W_p)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated Gene:YPR123C(YPR123C_d)|FD-Score:-3.23|P-value:6.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:ACB1(YGR037C)|FD-Score:5.2|P-value:9.76E-8||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:AEP1(YMR064W)|FD-Score:-3.11|P-value:9.32E-4||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:AIM22(YJL046W)|FD-Score:-4.35|P-value:6.79E-6||SGD DESC:Putative lipoate-protein ligase, required along with Lip2 and Lip5 for lipoylation of Lat1p and Kgd2p; similar to E. coli LplA; null mutant displays reduced frequency of mitochondrial genome loss Gene:ASN1(YPR145W)|FD-Score:4.98|P-value:3.18E-7||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication Gene:BIK1(YCL029C)|FD-Score:-3.1|P-value:9.53E-4||SGD DESC:Microtubule-associated protein, component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170 Gene:BRE4(YDL231C)|FD-Score:4.45|P-value:4.25E-6||SGD DESC:Zinc finger protein containing five transmembrane domains; null mutant exhibits strongly fragmented vacuoles and sensitivity to brefeldin A, a drug which is known to affect intracellular transport Gene:BUD25(YER014C-A)|FD-Score:-3.91|P-value:4.71E-5||SGD DESC:Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern Gene:CAF4(YKR036C)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:WD40 repeat-containing protein associated with the CCR4-NOT complex; interacts in a Ccr4p-dependent manner with Ssn2p; also interacts with Fis1p, Mdv1p and Dnm1p and plays a role in mitochondrial fission; CAF4 has a paralog, MDV1, that arose from the whole genome duplication Gene:CAF40(YNL288W)|FD-Score:-3.35|P-value:4.07E-4||SGD DESC:Evolutionarily conserved subunit of the CCR4-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p Gene:CBT1(YKL208W)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Protein involved in 5' end processing of mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p Gene:COQ1(YBR003W)|FD-Score:-3.28|P-value:5.24E-4||SGD DESC:Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis Gene:COS111(YBR203W)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:COX10(YPL172C)|FD-Score:-4.01|P-value:3.02E-5||SGD DESC:Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders Gene:COX18(YGR062C)|FD-Score:-3.61|P-value:1.56E-4||SGD DESC:Mitochondrial integral inner membrane protein required for membrane insertion of C-terminus of Cox2p; interacts genetically and physically with Mss2p and Pnt1p; similar to S. cerevisiae Oxa1, N. crassa Oxa2p, and E. coli YidC Gene:DMC1(YER179W)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Meiosis-specific protein required for repair of double-strand breaks and pairing between homologous chromosomes; homolog of Rad51p and the bacterial RecA protein; binds ssDNA and dsDNA, forms helical filaments; stimulated by Rdh54p Gene:DPH1(YIL103W)|FD-Score:-3.49|P-value:2.44E-4||SGD DESC:Protein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p Gene:ECM33(YBR078W)|FD-Score:-8.54|P-value:6.81E-18||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:ELP3(YPL086C)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin Gene:FAU1(YER183C)|FD-Score:4|P-value:3.12E-5||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:GAL80(YML051W)|FD-Score:-4.25|P-value:1.07E-5||SGD DESC:Transcriptional regulator involved in the repression of GAL genes in the absence of galactose; inhibits transcriptional activation by Gal4p; inhibition relieved by Gal3p or Gal1p binding Gene:GCG1(YER163C_p)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle Gene:GDA1(YEL042W)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate Gene:GEP3(YOR205C)|FD-Score:-3.14|P-value:8.43E-4||SGD DESC:Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (phb1) Gene:GIR2(YDR152W)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Highly-acidic cytoplasmic RWD domain-containing protein of unknown function; forms a complex with Rbg2p; interacts with Rbg1p and Gcn1p; associates with translating ribosomes; putative intrinsically unstructured protein Gene:GLG1(YKR058W)|FD-Score:3.15|P-value:8.15E-4||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG1 has a paralog, GLG2, that arose from the whole genome duplication Gene:GTF1(YGR102C)|FD-Score:-3.78|P-value:7.96E-5||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; transposon insertion mutant is salt sensitive and null mutant has growth defects; non-tagged protein is detected in purified mitochondria Gene:GUP1(YGL084C)|FD-Score:-3.65|P-value:1.32E-4||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HAL9(YOL089C)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication Gene:IOC4(YMR044W)|FD-Score:4.02|P-value:2.93E-5||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing Gene:ISM1(YPL040C)|FD-Score:-3.54|P-value:2.00E-4||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:IZH2(YOL002C)|FD-Score:3.13|P-value:8.71E-4||SGD DESC:Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc Gene:MAC1(YMR021C)|FD-Score:-3.2|P-value:6.80E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MAL31(YBR298C)|FD-Score:-3.44|P-value:2.95E-4||SGD DESC:Maltose permease, high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL3 complex locus; member of the 12 transmembrane domain superfamily of sugar transporters; functional in genomic reference strain S288C Gene:MST1(YKL194C)|FD-Score:-4.07|P-value:2.31E-5||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:NGG1(YDR176W)|FD-Score:9.11|P-value:4.01E-20||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:NIT2(YJL126W)|FD-Score:4.1|P-value:2.11E-5||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:NUP42(YDR192C)|FD-Score:4.72|P-value:1.18E-6||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; interacts with Gle1p Gene:OAR1(YKL055C)|FD-Score:-3.25|P-value:5.84E-4||SGD DESC:Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p Gene:PMT2(YAL023C)|FD-Score:-4.76|P-value:9.54E-7||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication Gene:RAD17(YOR368W)|FD-Score:3.12|P-value:9.18E-4||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins Gene:RDI1(YDL135C)|FD-Score:5.57|P-value:1.31E-8||SGD DESC:Rho GDP dissociation inhibitor; involved in the localization and regulation of Cdc42p and Rho1p; protein abundance increases in response to DNA replication stress Gene:RNQ1(YCL028W)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:[PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate Gene:RPA49(YNL248C)|FD-Score:-3.79|P-value:7.42E-5||SGD DESC:RNA polymerase I subunit A49 Gene:RPL12A(YEL054C)|FD-Score:-3.12|P-value:9.17E-4||SGD DESC:Ribosomal 60S subunit protein L12A; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12A has a paralog, RPL12B, that arose from the whole genome duplication Gene:RPL22A(YLR061W)|FD-Score:-3.28|P-value:5.19E-4||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RSM22(YKL155C)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase Gene:RTC6(YPL183W-A)|FD-Score:-3.16|P-value:7.90E-4||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:SAC6(YDR129C)|FD-Score:-3.77|P-value:8.01E-5||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SDC1(YDR469W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 Gene:SLA1(YBL007C)|FD-Score:-3.58|P-value:1.74E-4||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SNF12(YNR023W)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive Gene:SNT309(YPR101W)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SOD1(YJR104C)|FD-Score:-3.29|P-value:4.98E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SSF1(YHR066W)|FD-Score:-3.54|P-value:2.04E-4||SGD DESC:Constituent of 66S pre-ribosomal particles; required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family; SSF1 has a paralog, SSF2, that arose from the whole genome duplication Gene:SSK2(YNR031C)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress Gene:TDA7(YNL176C_p)|FD-Score:5.22|P-value:8.90E-8||SGD DESC:Cell cycle-regulated gene of unknown function, promoter bound by Fkh2p; null mutant is sensitive to expression of the top1-T722A allele Gene:TIM8(YJR135W-A)|FD-Score:3.1|P-value:9.64E-4||SGD DESC:Mitochondrial intermembrane space protein, forms a complex with Tim13p that delivers a subset of hydrophobic proteins to the TIM22 complex for inner membrane insertion; homolog of human TIMM8A, implicated in Mohr-Tranebjaerg syndrome Gene:TMA16(YOR252W)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Protein of unknown function that associates with ribosomes Gene:TRI1(YMR233W)|FD-Score:4.46|P-value:4.06E-6||SGD DESC:Non-essential sumoylated protein of unknown function; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:3.73|P-value:9.75E-5||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VMA1(YDL185W)|FD-Score:-3.14|P-value:8.42E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VPS25(YJR102C)|FD-Score:-6.03|P-value:8.34E-10||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:YAL016C-B(YAL016C-B_d)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR154C(YDR154C_d)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein Gene:YEN1(YER041W)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p Gene:YER077C(YER077C_p)|FD-Score:-5.32|P-value:5.25E-8||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport Gene:YFL019C(YFL019C_d)|FD-Score:4.8|P-value:7.99E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Gene:YFR020W(YFR020W_p)|FD-Score:4.42|P-value:4.92E-6||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YML009W-B(YML009W-B_d)|FD-Score:-3.24|P-value:5.91E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Gene:YML122C(YML122C_d)|FD-Score:-4.82|P-value:7.15E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR318C(YOR318C_d)|FD-Score:3.86|P-value:5.67E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo Gene:YPL168W(YPL168W_p)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated Gene:YPR123C(YPR123C_d)|FD-Score:-3.23|P-value:6.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJL031C6.651.51E-110.88BET4Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p
YGL172W6.047.81E-100.88NUP49FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p)
YER112W5.161.23E-70.39LSM4Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress
YLR029C4.779.22E-70.06RPL15ARibosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication
YDR189W4.711.23E-60.41SLY1Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex
YLR078C4.308.54E-60.41BOS1v-SNARE (vesicle specific SNAP receptor), localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi
YJR013W3.894.94E-50.21GPI14Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
YDR168W3.691.13E-40.03CDC37Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding
YKL182W3.661.26E-40.19FAS1Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities
YPL210C3.472.57E-40.08SRP72Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane
YLR438C-A3.393.46E-40.10LSM3Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YBR211C3.295.00E-40.01AME1Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress
YBR055C3.285.21E-40.02PRP6Splicing factor, component of the U4/U6-U5 snRNP complex
YNL114C_d3.265.54E-40.00YNL114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit
YKR004C3.265.56E-40.01ECM9Non-essential protein of unknown function

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR176W9.114.01E-20NGG1Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex
YDL135C5.571.31E-8RDI1Rho GDP dissociation inhibitor; involved in the localization and regulation of Cdc42p and Rho1p; protein abundance increases in response to DNA replication stress
YNL176C_p5.228.90E-8TDA7_pCell cycle-regulated gene of unknown function, promoter bound by Fkh2p; null mutant is sensitive to expression of the top1-T722A allele
YGR037C5.209.76E-8ACB1Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress
YPR145W4.983.18E-7ASN1Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication
YFL019C_d4.807.99E-7YFL019C_dDubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene
YDR192C4.721.18E-6NUP42FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; interacts with Gle1p
YMR233W4.464.06E-6TRI1Non-essential sumoylated protein of unknown function; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication
YDL231C4.454.25E-6BRE4Zinc finger protein containing five transmembrane domains; null mutant exhibits strongly fragmented vacuoles and sensitivity to brefeldin A, a drug which is known to affect intracellular transport
YFR020W_p4.424.92E-6YFR020W_pProtein of unknown function; mRNA identified as translated by ribosome profiling data
YJL126W4.102.11E-5NIT2Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member
YMR044W4.022.93E-5IOC4Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing
YER183C4.003.12E-5FAU15,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis
YOR318C_d3.865.67E-5YOR318C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo
YBR058C-A3.739.75E-5TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis

GO enrichment analysis for SGTC_2905
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3457.81E-165SGTC_1469k284-2957 126.0 μMChemDiv (Drug-like library)238848410.150685
0.2041.71E-56SGTC_282k297-0036 65.7 μMChemDiv (Drug-like library)50613780.1
0.1087.23E-17SGTC_32439134147 49.5 μMChembridge (Drug-like library)242821800.125
0.0976.29E-14SGTC_810411-0046 10.0 μMChemDiv (Drug-like library)129252650.137931
0.0979.48E-14SGTC_22176934799 96.1 μMChembridge (Fragment library)6756060.112903TSC3-RPN4
0.0921.18E-12SGTC_692k241-0027 77.8 μMChemDiv (Drug-like library)36004800.0923077
0.0912.25E-12SGTC_266formosanin C 406.0 nMMiscellaneous735970.0338983sphingolipid biosynthesis & PDR1
0.0855.31E-11SGTC_1568digitonin 820.0 nMTimTec (Pure natural product library)27350100.0165289sphingolipid biosynthesis & PDR1
0.0808.96E-10SGTC_9661300-0360 30.2 μMChemDiv (Drug-like library)705880.0483871heme requiring
0.0799.92E-10SGTC_1785bathophenanthroline 24.0 μMMiscellaneous728120.0535714heme requiring
0.0781.68E-9SGTC_1471k673-0312 37.0 μMChemDiv (Drug-like library)240998840.157143RNA processing & uracil transport
0.0781.87E-9SGTC_28589025781 9.0 μMChembridge (Drug-like library)48949800.0724638sphingolipid biosynthesis & PDR1
0.0781.92E-9SGTC_490phenamil 164.0 μMMiscellaneous47550.153846heme requiring
0.0765.99E-9SGTC_13201313-0481 95.3 μMChemDiv (Drug-like library)68133650.135802
0.0732.27E-8SGTC_2261,3-diallylurea 8.3 mMMiscellaneous745370.104167

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_22077258803200 μM0.5348843666799Chembridge (Fragment library)244.08521.68212
SGTC_1035k081-004155.2 μM0.392857731709ChemDiv (Drug-like library)341.58772.8613Golgi
SGTC_22436634756200 μM0.387755885676Chembridge (Fragment library)234.079262.01512
SGTC_8261683-5028118 μM0.333333722377ChemDiv (Drug-like library)261.703583.26212
SGTC_3298911685871.43 μM0.3333332506942Chembridge (Drug-like library)279.718862.65713
SGTC_22537938677135.83 μM0.3137262301972Chembridge (Fragment library)229.660181.66913
SGTC_3195911308249.47 μM0.30909117018038Chembridge (Drug-like library)301.337063.05114
SGTC_12201838-0075193 μM0.3064525529645ChemDiv (Drug-like library)379.20533.15625
SGTC_9791426-2828121 μM0.303571747872ChemDiv (Drug-like library)270.114662.88412
SGTC_2787528454071.43 μM0.303571736958Chembridge (Drug-like library)291.729563.24613