7948405

6-bromo-3-chloro-N-(3-morpholin-4-ylpropyl)-1-benzothiophene-2-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2910
Screen concentration 26.0 μM
Source Chembridge (Drug-like library)
PubChem CID 2971328
SMILES C1COCCN1CCCNC(=O)C2=C(C3=C(S2)C=C(C=C3)Br)Cl
Standardized SMILES Clc1c(sc2cc(Br)ccc12)C(=O)NCCCN3CCOCC3
Molecular weight 417.7483
ALogP 3.49
H-bond donor count 1
H-bond acceptor count 4
Response signature plasma membrane duress

Pool Growth Kinetics
% growth inhibition (Het. pool) 4.52
% growth inhibition (Hom. pool) 5.48


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2971328
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:-4.89|P-value:5.09E-7|Clearance:0||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:BOS1(YLR078C)|FD-Score:-3.15|P-value:8.10E-4|Clearance:0||SGD DESC:v-SNARE (vesicle specific SNAP receptor), localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi Gene:FIP1(YJR093C)|FD-Score:-3.18|P-value:7.46E-4|Clearance:0||SGD DESC:Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p Gene:GCD2(YGR083C)|FD-Score:-3.56|P-value:1.89E-4|Clearance:0||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:HEM12(YDR047W)|FD-Score:4.11|P-value:1.94E-5|Clearance:0.18||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:NAM9(YNL137C)|FD-Score:3.51|P-value:2.23E-4|Clearance:0.14||SGD DESC:Mitochondrial ribosomal component of the small subunit Gene:NFS1(YCL017C)|FD-Score:-3.32|P-value:4.56E-4|Clearance:0||SGD DESC:Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria Gene:OLE1(YGL055W)|FD-Score:7.41|P-value:6.23E-14|Clearance:2.42||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PGA1(YNL158W)|FD-Score:3.35|P-value:4.07E-4|Clearance:0.19||SGD DESC:Essential component of GPI-mannosyltransferase II, responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interations with secretory pathway genes Gene:QNS1(YHR074W)|FD-Score:4.99|P-value:2.96E-7|Clearance:0.88||SGD DESC:Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide Gene:ROT1(YMR200W)|FD-Score:3.94|P-value:4.09E-5|Clearance:0.43||SGD DESC:Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation Gene:RPC53(YDL150W)|FD-Score:-3.27|P-value:5.30E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C53 Gene:RRP9(YPR137W)|FD-Score:-3.2|P-value:6.87E-4|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:SEC5(YDR166C)|FD-Score:3.16|P-value:7.86E-4|Clearance:0.14||SGD DESC:Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip Gene:UBA1(YKL210W)|FD-Score:3.37|P-value:3.80E-4|Clearance:0.02||SGD DESC:Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress Gene:YGR265W(YGR265W_d)|FD-Score:-3.45|P-value:2.85E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase Gene:ACT1(YFL039C)|FD-Score:-4.89|P-value:5.09E-7|Clearance:0||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:BOS1(YLR078C)|FD-Score:-3.15|P-value:8.10E-4|Clearance:0||SGD DESC:v-SNARE (vesicle specific SNAP receptor), localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi Gene:FIP1(YJR093C)|FD-Score:-3.18|P-value:7.46E-4|Clearance:0||SGD DESC:Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p Gene:GCD2(YGR083C)|FD-Score:-3.56|P-value:1.89E-4|Clearance:0||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:HEM12(YDR047W)|FD-Score:4.11|P-value:1.94E-5|Clearance:0.18||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:NAM9(YNL137C)|FD-Score:3.51|P-value:2.23E-4|Clearance:0.14||SGD DESC:Mitochondrial ribosomal component of the small subunit Gene:NFS1(YCL017C)|FD-Score:-3.32|P-value:4.56E-4|Clearance:0||SGD DESC:Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria Gene:OLE1(YGL055W)|FD-Score:7.41|P-value:6.23E-14|Clearance:2.42||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PGA1(YNL158W)|FD-Score:3.35|P-value:4.07E-4|Clearance:0.19||SGD DESC:Essential component of GPI-mannosyltransferase II, responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interations with secretory pathway genes Gene:QNS1(YHR074W)|FD-Score:4.99|P-value:2.96E-7|Clearance:0.88||SGD DESC:Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide Gene:ROT1(YMR200W)|FD-Score:3.94|P-value:4.09E-5|Clearance:0.43||SGD DESC:Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation Gene:RPC53(YDL150W)|FD-Score:-3.27|P-value:5.30E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C53 Gene:RRP9(YPR137W)|FD-Score:-3.2|P-value:6.87E-4|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:SEC5(YDR166C)|FD-Score:3.16|P-value:7.86E-4|Clearance:0.14||SGD DESC:Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip Gene:UBA1(YKL210W)|FD-Score:3.37|P-value:3.80E-4|Clearance:0.02||SGD DESC:Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress Gene:YGR265W(YGR265W_d)|FD-Score:-3.45|P-value:2.85E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2971328
Download HOP data (tab-delimited text)  (excel)
Gene:ALD6(YPL061W)|FD-Score:4.12|P-value:1.89E-5||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:ARC18(YLR370C)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARN2(YHL047C)|FD-Score:3.99|P-value:3.25E-5||SGD DESC:Transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to the siderophore triacetylfusarinine C Gene:ARO1(YDR127W)|FD-Score:5.82|P-value:3.01E-9||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:5.53|P-value:1.63E-8||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ATG15(YCR068W)|FD-Score:4.92|P-value:4.41E-7||SGD DESC:Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway Gene:ATG20(YDL113C)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Sorting nexin family member required for the cytoplasm-to-vacuole targeting (Cvt) pathway and for endosomal sorting; has a Phox homology domain that binds phosphatidylinositol-3-phosphate; interacts with Snx4p; potential Cdc28p substrate Gene:BOR1(YNL275W)|FD-Score:4.96|P-value:3.57E-7||SGD DESC:Boron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the characterized boron efflux transporter A. thaliana BOR1 Gene:BSC4(YNL269W)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p Gene:CDC50(YCR094W)|FD-Score:22.5|P-value:3.03E-112||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CLA4(YNL298W)|FD-Score:4.4|P-value:5.48E-6||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:COG5(YNL051W)|FD-Score:-3.32|P-value:4.44E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG6(YNL041C)|FD-Score:-3.29|P-value:5.02E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CYS3(YAL012W)|FD-Score:7.31|P-value:1.31E-13||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DRS2(YAL026C)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:ECM19(YLR390W)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:ENV10(YLR065C)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:ERC1(YHR032W)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily; overproduction confers ethionine resistance and accumulation of S-adenosylmethionine Gene:FEN2(YCR028C)|FD-Score:-3.59|P-value:1.62E-4||SGD DESC:Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:GCN3(YKR026C)|FD-Score:4.24|P-value:1.09E-5||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCS1(YDL226C)|FD-Score:9.35|P-value:4.37E-21||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:HEM14(YER014W)|FD-Score:5.14|P-value:1.35E-7||SGD DESC:Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides Gene:HOS1(YPR068C)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Class I histone deacetylase (HDAC) family member that deacetylates Smc3p on lysine residues at anaphase onset; has sequence similarity to Hda1p, Rpd3p, Hos2p, and Hos3p; interacts with the Tup1p-Ssn6p corepressor complex Gene:HTL1(YCR020W-B)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:IDP3(YNL009W)|FD-Score:3.21|P-value:6.74E-4||SGD DESC:Peroxisomal NADP-dependent isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids; IDP3 has a paralog, IDP2, that arose from the whole genome duplication Gene:IOC4(YMR044W)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing Gene:IRC21(YMR073C)|FD-Score:4.35|P-value:6.70E-6||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:ISR1(YPR106W)|FD-Score:3.47|P-value:2.65E-4||SGD DESC:Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C Gene:ITR2(YOL103W)|FD-Score:-3.17|P-value:7.71E-4||SGD DESC:Myo-inositol transporter with strong similarity to the major myo-inositol transporter Itr1p, member of the sugar transporter superfamily; expressed constitutively Gene:LCL1(YPL056C_p)|FD-Score:-3.22|P-value:6.50E-4||SGD DESC:Putative protein of unknown function; deletion mutant is fluconazole resistant and has long chronological lifespan Gene:LEA1(YPL213W)|FD-Score:-3.54|P-value:1.97E-4||SGD DESC:Component of U2 snRNP; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein Gene:LIN1(YHR156C)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication Gene:MET16(YPR167C)|FD-Score:-3.93|P-value:4.17E-5||SGD DESC:3'-phosphoadenylsulfate reductase, reduces 3'-phosphoadenylyl sulfate to adenosine-3',5'-bisphosphate and free sulfite using reduced thioredoxin as cosubstrate, involved in sulfate assimilation and methionine metabolism Gene:MGM1(YOR211C)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Mitochondrial GTPase, present in complex with Ugo1p and Fzo1p; required for mt morphology and genome maintenance; exists as long and short form with different distributions; homolog of human OPA1 involved in autosomal dominant optic atrophy Gene:MRPL32(YCR003W)|FD-Score:3.78|P-value:7.94E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:OPI7(YDR360W_d)|FD-Score:3.14|P-value:8.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene VID21/YDR359C. Gene:PEP12(YOR036W)|FD-Score:3.94|P-value:4.15E-5||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PRO1(YDR300C)|FD-Score:-5.8|P-value:3.24E-9||SGD DESC:Gamma-glutamyl kinase; catalyzes the first step in proline biosynthesis; PRO1 has a paralog, YHR033W, that arose from the whole genome duplication Gene:RCY1(YJL204C)|FD-Score:6.77|P-value:6.35E-12||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RIC1(YLR039C)|FD-Score:-3.36|P-value:3.83E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:ROY1(YMR258C)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p Gene:RPL40B(YKR094C)|FD-Score:-3.38|P-value:3.59E-4||SGD DESC:Ubiquitin-ribosomal 60S subunit protein L40B fusion protein; cleaved to yield ubiquitin and ribosomal protein L40B; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40B has a paralog, RPL40A, that arose from the whole genome duplication Gene:RSM7(YJR113C)|FD-Score:3.81|P-value:7.00E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:SAC6(YDR129C)|FD-Score:-3.68|P-value:1.19E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SEA4(YBL104C)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SNF2(YOR290C)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SPO12(YHR152W)|FD-Score:5.03|P-value:2.40E-7||SGD DESC:Nucleolar protein of unknown function, positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis Gene:SSQ1(YLR369W)|FD-Score:3.09|P-value:9.91E-4||SGD DESC:Mitochondrial hsp70-type molecular chaperone, required for assembly of iron/sulfur clusters into proteins at a step after cluster synthesis, and for maturation of Yfh1p, which is a homolog of human frataxin implicated in Friedreich's ataxia Gene:SWA2(YDR320C)|FD-Score:-6.46|P-value:5.07E-11||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:THI74(YDR438W)|FD-Score:3.25|P-value:5.81E-4||SGD DESC:Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication Gene:TMA108(YIL137C)|FD-Score:4.57|P-value:2.42E-6||SGD DESC:Protein that associates with ribosomes and is involved in ribosome biogenesis; putative metalloprotease Gene:TRP1(YDR007W)|FD-Score:4.13|P-value:1.80E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:4.89|P-value:5.16E-7||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:8.01|P-value:5.89E-16||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:8.03|P-value:4.93E-16||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:UBP12(YJL197W)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; present in the nucleus and cytoplasm Gene:VPS1(YKR001C)|FD-Score:-4.01|P-value:3.09E-5||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS28(YPL065W)|FD-Score:4.18|P-value:1.46E-5||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS45(YGL095C)|FD-Score:-3.69|P-value:1.12E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS61(YDR136C_d)|FD-Score:-3.8|P-value:7.12E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YCL074W(YCL074W)|FD-Score:-5.53|P-value:1.61E-8||SGD DESC:Pseudogene: encodes fragment of Ty Pol protein Gene:YDL071C(YDL071C_d)|FD-Score:-6.49|P-value:4.32E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF BDF2/YDL070W Gene:YDL119C(YDL119C_p)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria Gene:YDR008C(YDR008C_d)|FD-Score:7.34|P-value:1.09E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR253C(YMR253C_p)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene Gene:YNL266W(YNL266W_d)|FD-Score:5.17|P-value:1.16E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF IST1/YNL265C Gene:YNR073C(YNR073C_p)|FD-Score:-3.33|P-value:4.28E-4||SGD DESC:Putative mannitol dehydrogenase; identical in amino acid sequence to Dsf1p Gene:YOL050C(YOL050C_d)|FD-Score:3.2|P-value:6.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YOR072W(YOR072W_d)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPR089W(YPR089W_p)|FD-Score:-3.44|P-value:2.92E-4||SGD DESC:Protein of unknown function; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p Gene:YPR098C(YPR098C)|FD-Score:5.23|P-value:8.49E-8||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YPT52(YKR014C)|FD-Score:3.25|P-value:5.77E-4||SGD DESC:Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress Gene:YPT6(YLR262C)|FD-Score:-3.23|P-value:6.10E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:ZAP1(YJL056C)|FD-Score:-3.11|P-value:9.44E-4||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains Gene:ALD6(YPL061W)|FD-Score:4.12|P-value:1.89E-5||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:ARC18(YLR370C)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARN2(YHL047C)|FD-Score:3.99|P-value:3.25E-5||SGD DESC:Transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to the siderophore triacetylfusarinine C Gene:ARO1(YDR127W)|FD-Score:5.82|P-value:3.01E-9||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:5.53|P-value:1.63E-8||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ATG15(YCR068W)|FD-Score:4.92|P-value:4.41E-7||SGD DESC:Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway Gene:ATG20(YDL113C)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Sorting nexin family member required for the cytoplasm-to-vacuole targeting (Cvt) pathway and for endosomal sorting; has a Phox homology domain that binds phosphatidylinositol-3-phosphate; interacts with Snx4p; potential Cdc28p substrate Gene:BOR1(YNL275W)|FD-Score:4.96|P-value:3.57E-7||SGD DESC:Boron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the characterized boron efflux transporter A. thaliana BOR1 Gene:BSC4(YNL269W)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p Gene:CDC50(YCR094W)|FD-Score:22.5|P-value:3.03E-112||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CLA4(YNL298W)|FD-Score:4.4|P-value:5.48E-6||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:COG5(YNL051W)|FD-Score:-3.32|P-value:4.44E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG6(YNL041C)|FD-Score:-3.29|P-value:5.02E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CYS3(YAL012W)|FD-Score:7.31|P-value:1.31E-13||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DRS2(YAL026C)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:ECM19(YLR390W)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:ENV10(YLR065C)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:ERC1(YHR032W)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily; overproduction confers ethionine resistance and accumulation of S-adenosylmethionine Gene:FEN2(YCR028C)|FD-Score:-3.59|P-value:1.62E-4||SGD DESC:Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:GCN3(YKR026C)|FD-Score:4.24|P-value:1.09E-5||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCS1(YDL226C)|FD-Score:9.35|P-value:4.37E-21||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:HEM14(YER014W)|FD-Score:5.14|P-value:1.35E-7||SGD DESC:Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides Gene:HOS1(YPR068C)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Class I histone deacetylase (HDAC) family member that deacetylates Smc3p on lysine residues at anaphase onset; has sequence similarity to Hda1p, Rpd3p, Hos2p, and Hos3p; interacts with the Tup1p-Ssn6p corepressor complex Gene:HTL1(YCR020W-B)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:IDP3(YNL009W)|FD-Score:3.21|P-value:6.74E-4||SGD DESC:Peroxisomal NADP-dependent isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids; IDP3 has a paralog, IDP2, that arose from the whole genome duplication Gene:IOC4(YMR044W)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing Gene:IRC21(YMR073C)|FD-Score:4.35|P-value:6.70E-6||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:ISR1(YPR106W)|FD-Score:3.47|P-value:2.65E-4||SGD DESC:Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C Gene:ITR2(YOL103W)|FD-Score:-3.17|P-value:7.71E-4||SGD DESC:Myo-inositol transporter with strong similarity to the major myo-inositol transporter Itr1p, member of the sugar transporter superfamily; expressed constitutively Gene:LCL1(YPL056C_p)|FD-Score:-3.22|P-value:6.50E-4||SGD DESC:Putative protein of unknown function; deletion mutant is fluconazole resistant and has long chronological lifespan Gene:LEA1(YPL213W)|FD-Score:-3.54|P-value:1.97E-4||SGD DESC:Component of U2 snRNP; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein Gene:LIN1(YHR156C)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication Gene:MET16(YPR167C)|FD-Score:-3.93|P-value:4.17E-5||SGD DESC:3'-phosphoadenylsulfate reductase, reduces 3'-phosphoadenylyl sulfate to adenosine-3',5'-bisphosphate and free sulfite using reduced thioredoxin as cosubstrate, involved in sulfate assimilation and methionine metabolism Gene:MGM1(YOR211C)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Mitochondrial GTPase, present in complex with Ugo1p and Fzo1p; required for mt morphology and genome maintenance; exists as long and short form with different distributions; homolog of human OPA1 involved in autosomal dominant optic atrophy Gene:MRPL32(YCR003W)|FD-Score:3.78|P-value:7.94E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:OPI7(YDR360W_d)|FD-Score:3.14|P-value:8.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene VID21/YDR359C. Gene:PEP12(YOR036W)|FD-Score:3.94|P-value:4.15E-5||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PRO1(YDR300C)|FD-Score:-5.8|P-value:3.24E-9||SGD DESC:Gamma-glutamyl kinase; catalyzes the first step in proline biosynthesis; PRO1 has a paralog, YHR033W, that arose from the whole genome duplication Gene:RCY1(YJL204C)|FD-Score:6.77|P-value:6.35E-12||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RIC1(YLR039C)|FD-Score:-3.36|P-value:3.83E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:ROY1(YMR258C)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p Gene:RPL40B(YKR094C)|FD-Score:-3.38|P-value:3.59E-4||SGD DESC:Ubiquitin-ribosomal 60S subunit protein L40B fusion protein; cleaved to yield ubiquitin and ribosomal protein L40B; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40B has a paralog, RPL40A, that arose from the whole genome duplication Gene:RSM7(YJR113C)|FD-Score:3.81|P-value:7.00E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:SAC6(YDR129C)|FD-Score:-3.68|P-value:1.19E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SEA4(YBL104C)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SNF2(YOR290C)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SPO12(YHR152W)|FD-Score:5.03|P-value:2.40E-7||SGD DESC:Nucleolar protein of unknown function, positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis Gene:SSQ1(YLR369W)|FD-Score:3.09|P-value:9.91E-4||SGD DESC:Mitochondrial hsp70-type molecular chaperone, required for assembly of iron/sulfur clusters into proteins at a step after cluster synthesis, and for maturation of Yfh1p, which is a homolog of human frataxin implicated in Friedreich's ataxia Gene:SWA2(YDR320C)|FD-Score:-6.46|P-value:5.07E-11||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:THI74(YDR438W)|FD-Score:3.25|P-value:5.81E-4||SGD DESC:Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication Gene:TMA108(YIL137C)|FD-Score:4.57|P-value:2.42E-6||SGD DESC:Protein that associates with ribosomes and is involved in ribosome biogenesis; putative metalloprotease Gene:TRP1(YDR007W)|FD-Score:4.13|P-value:1.80E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:4.89|P-value:5.16E-7||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:8.01|P-value:5.89E-16||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:8.03|P-value:4.93E-16||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:UBP12(YJL197W)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; present in the nucleus and cytoplasm Gene:VPS1(YKR001C)|FD-Score:-4.01|P-value:3.09E-5||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS28(YPL065W)|FD-Score:4.18|P-value:1.46E-5||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS45(YGL095C)|FD-Score:-3.69|P-value:1.12E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS61(YDR136C_d)|FD-Score:-3.8|P-value:7.12E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YCL074W(YCL074W)|FD-Score:-5.53|P-value:1.61E-8||SGD DESC:Pseudogene: encodes fragment of Ty Pol protein Gene:YDL071C(YDL071C_d)|FD-Score:-6.49|P-value:4.32E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF BDF2/YDL070W Gene:YDL119C(YDL119C_p)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria Gene:YDR008C(YDR008C_d)|FD-Score:7.34|P-value:1.09E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR253C(YMR253C_p)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene Gene:YNL266W(YNL266W_d)|FD-Score:5.17|P-value:1.16E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF IST1/YNL265C Gene:YNR073C(YNR073C_p)|FD-Score:-3.33|P-value:4.28E-4||SGD DESC:Putative mannitol dehydrogenase; identical in amino acid sequence to Dsf1p Gene:YOL050C(YOL050C_d)|FD-Score:3.2|P-value:6.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YOR072W(YOR072W_d)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPR089W(YPR089W_p)|FD-Score:-3.44|P-value:2.92E-4||SGD DESC:Protein of unknown function; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p Gene:YPR098C(YPR098C)|FD-Score:5.23|P-value:8.49E-8||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YPT52(YKR014C)|FD-Score:3.25|P-value:5.77E-4||SGD DESC:Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress Gene:YPT6(YLR262C)|FD-Score:-3.23|P-value:6.10E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:ZAP1(YJL056C)|FD-Score:-3.11|P-value:9.44E-4||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL055W7.416.23E-142.42OLE1Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
YHR074W4.992.96E-70.88QNS1Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide
YDR047W4.111.94E-50.18HEM12Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
YMR200W3.944.09E-50.43ROT1Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation
YNL137C3.512.23E-40.14NAM9Mitochondrial ribosomal component of the small subunit
YKL210W3.373.80E-40.02UBA1Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress
YNL158W3.354.07E-40.19PGA1Essential component of GPI-mannosyltransferase II, responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interations with secretory pathway genes
YDR166C3.167.86E-40.14SEC5Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip
YDR082W3.020.001270.05STN1Telomere end-binding and capping protein, plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping
YOL094C2.970.001480.03RFC4Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YLR438C-A2.940.001640.09LSM3Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YBR102C2.850.002190.06EXO84Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate
YGL116W2.790.002620.05CDC20Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
YLR088W2.740.003060.11GAA1Subunit of the GPI (glycosylphosphatidylinositol):protein transamidase complex, removes the GPI-anchoring signal and attaches GPI to proteins in the ER
YKL058W2.630.004260.02TOA2TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YCR094W22.503.03E-112CDC50Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication
YDL226C9.354.37E-21GCS1ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p
YDR354W8.034.93E-16TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YKL211C8.015.89E-16TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YDR008C_d7.341.09E-13YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YAL012W7.311.31E-13CYS3Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress
YJL204C6.776.35E-12RCY1F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
YDR127W5.823.01E-9ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YGL148W5.531.63E-8ARO2Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress
YPR098C5.238.49E-8YPR098CProtein of unknown function, localized to the mitochondrial outer membrane
YNL266W_d5.171.16E-7YNL266W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF IST1/YNL265C
YER014W5.141.35E-7HEM14Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides
YHR152W5.032.40E-7SPO12Nucleolar protein of unknown function, positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis
YNL275W4.963.57E-7BOR1Boron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the characterized boron efflux transporter A. thaliana BOR1
YCR068W4.924.41E-7ATG15Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway

GO enrichment analysis for SGTC_2910
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.4051.54E-231SGTC_2725haloperidol 53.2 μMMiscellaneous35590.220779plasma membrane duress
0.3881.27E-211SGTC_1999st074831 12.5 μMTimTec (Natural product derivative library)171125230.160494plasma membrane duress
0.3834.94E-206SGTC_20384100038 31.0 μMChembridge (Fragment library)35968030.0821918plasma membrane duress
0.3713.20E-191SGTC_2721ifenprodil 61.4 μMMiscellaneous36890.0853659plasma membrane duress
0.3693.61E-190SGTC_2733amorolfine 100.0 μMMiscellaneous542600.0987654plasma membrane duress
0.3437.56E-163SGTC_14354092-0789 56.7 μMChemDiv (Drug-like library)22482850.131579plasma membrane duress
0.3351.06E-154SGTC_29489070189 9.4 μMChembridge (Drug-like library)64736360.17284plasma membrane duress
0.3351.23E-154SGTC_2540epiandrosterone 100.0 μMTimTec (Pure natural product library)4413020.0352941
0.3265.92E-146SGTC_14554262-0120 131.0 μMChemDiv (Drug-like library)15295870.121212plasma membrane duress
0.3212.29E-141SGTC_2607solasodine 47.4 μMMicrosource (Natural product library)67106420.038835
0.3214.07E-141SGTC_29939054257 71.4 μMChembridge (Drug-like library)64702170.191781fatty acid desaturase (OLE1)
0.3196.06E-140SGTC_1649st011737 51.3 μMTimTec (Natural product derivative library)17610350.0823529
0.3161.29E-136SGTC_2735sertaconazole 140.0 nMMiscellaneous658630.0947368plasma membrane duress
0.3126.84E-133SGTC_30189082602 49.5 μMChembridge (Drug-like library)173320890.103896
0.3091.57E-130SGTC_28879044784 58.4 μMChembridge (Drug-like library)64655460.16fatty acid desaturase (OLE1)

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3154910011349.47 μM0.54838725237525Chembridge (Drug-like library)336.879444.39713
SGTC_3141909460949.47 μM0.5245925236309Chembridge (Drug-like library)381.67474.03713iron homeostasis
SGTC_297490944409.66 μM0.50793725236134Chembridge (Drug-like library)387.722343.71413ergosterol biosynthesis
SGTC_297190921869.93 μM0.3880625236400Chembridge (Drug-like library)343.271343.6313ergosterol biosynthesis
SGTC_2973909466517.96 μM0.38571425237061Chembridge (Drug-like library)336.879443.90813NEO1
SGTC_3152909925349.47 μM0.38235325236131Chembridge (Drug-like library)322.852863.45213NEO1-PIK1
SGTC_2898905627058.44 μM0.33333319154699Chembridge (Drug-like library)352.38381.80215
SGTC_2938905974627.26 μM0.3333336471769Chembridge (Drug-like library)452.21334.18434fatty acid desaturase (OLE1)
SGTC_3057909594649.47 μM0.33333324540043Chembridge (Drug-like library)311.8021034.43603Golgi
SGTC_3155909926549.47 μM0.32394425236499Chembridge (Drug-like library)330.83184.15213