7978188

5-chloro-3-hydroxy-3-[2-(2-hydroxyphenyl)-2-oxoethyl]-1-methylindol-2-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2919
Screen concentration 46.4 μM
Source Chembridge (Drug-like library)
PubChem CID 2980291
SMILES CN1C2=C(C=C(C=C2)Cl)C(C1=O)(CC(=O)C3=CC=CC=C3O)O
Standardized SMILES CN1C(=O)C(O)(CC(=O)c2ccccc2O)c3cc(Cl)ccc13
Molecular weight 331.7504
ALogP 2.26
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.06
% growth inhibition (Hom. pool) 3.26


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2980291
Download HIP data (tab-delimited text)  (excel)
Gene:KRE29(YER038C)|FD-Score:3.22|P-value:6.43E-4|Clearance:0.21||SGD DESC:Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance Gene:ORC2(YBR060C)|FD-Score:6.01|P-value:9.47E-10|Clearance:1.87||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p Gene:ORC6(YHR118C)|FD-Score:3.71|P-value:1.03E-4|Clearance:0.15||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p Gene:PRP28(YDR243C)|FD-Score:3.33|P-value:4.34E-4|Clearance:0.11||SGD DESC:RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site Gene:PTR3(YFR029W)|FD-Score:4.14|P-value:1.72E-5|Clearance:0.06||SGD DESC:Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes Gene:RIO2(YNL207W)|FD-Score:4.08|P-value:2.28E-5|Clearance:0.37||SGD DESC:Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p Gene:RPC40(YPR110C)|FD-Score:-5.81|P-value:3.18E-9|Clearance:0||SGD DESC:RNA polymerase subunit AC40, common to RNA polymerase I and III Gene:SCM3(YDL139C)|FD-Score:-5.02|P-value:2.62E-7|Clearance:0||SGD DESC:Nonhistone component of centromeric chromatin; binds stoichiometrically to CenH3-H4 histones, required for kinetochore assembly; required for G2/M progression and localization of Cse4p; may protect Cse4p from ubiquitylation; homolog of mammalian HJURP Gene:SEC6(YIL068C)|FD-Score:3.56|P-value:1.84E-4|Clearance:0.13||SGD DESC:Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p Gene:SUP35(YDR172W)|FD-Score:3.35|P-value:3.97E-4|Clearance:0.02||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:YPL251W(YPL251W_d)|FD-Score:3.43|P-value:2.99E-4|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C Gene:YPR142C(YPR142C_d)|FD-Score:-3.29|P-value:5.07E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing Gene:KRE29(YER038C)|FD-Score:3.22|P-value:6.43E-4|Clearance:0.21||SGD DESC:Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance Gene:ORC2(YBR060C)|FD-Score:6.01|P-value:9.47E-10|Clearance:1.87||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p Gene:ORC6(YHR118C)|FD-Score:3.71|P-value:1.03E-4|Clearance:0.15||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p Gene:PRP28(YDR243C)|FD-Score:3.33|P-value:4.34E-4|Clearance:0.11||SGD DESC:RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site Gene:PTR3(YFR029W)|FD-Score:4.14|P-value:1.72E-5|Clearance:0.06||SGD DESC:Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes Gene:RIO2(YNL207W)|FD-Score:4.08|P-value:2.28E-5|Clearance:0.37||SGD DESC:Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p Gene:RPC40(YPR110C)|FD-Score:-5.81|P-value:3.18E-9|Clearance:0||SGD DESC:RNA polymerase subunit AC40, common to RNA polymerase I and III Gene:SCM3(YDL139C)|FD-Score:-5.02|P-value:2.62E-7|Clearance:0||SGD DESC:Nonhistone component of centromeric chromatin; binds stoichiometrically to CenH3-H4 histones, required for kinetochore assembly; required for G2/M progression and localization of Cse4p; may protect Cse4p from ubiquitylation; homolog of mammalian HJURP Gene:SEC6(YIL068C)|FD-Score:3.56|P-value:1.84E-4|Clearance:0.13||SGD DESC:Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p Gene:SUP35(YDR172W)|FD-Score:3.35|P-value:3.97E-4|Clearance:0.02||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:YPL251W(YPL251W_d)|FD-Score:3.43|P-value:2.99E-4|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C Gene:YPR142C(YPR142C_d)|FD-Score:-3.29|P-value:5.07E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2980291
Download HOP data (tab-delimited text)  (excel)
Gene:ADE5,7(YGL234W)|FD-Score:3.85|P-value:5.94E-5||SGD DESC:Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities Gene:AIM37(YNL100W)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Mitochondrial inner membrane protein; component of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:APE3(YBR286W)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Vacuolar aminopeptidase Y, processed to mature form by Prb1p Gene:ARC1(YGL105W)|FD-Score:-4.24|P-value:1.14E-5||SGD DESC:Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress Gene:ATG13(YPR185W)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Regulatory subunit of the Atg1p signaling complex; stimulates Atg1p kinase activity; required for vesicle formation during autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; involved in Atg9p, Atg23p, and Atg27p cycling Gene:ATG17(YLR423C)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells Gene:CBP1(YJL209W)|FD-Score:4.63|P-value:1.79E-6||SGD DESC:Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress Gene:CCW14(YLR390W-A)|FD-Score:3.18|P-value:7.27E-4||SGD DESC:Covalently linked cell wall glycoprotein, present in the inner layer of the cell wall Gene:COA4(YLR218C)|FD-Score:3.29|P-value:4.97E-4||SGD DESC:Twin Cx(9)C protein involved in cytochrome c oxidase assembly or stability; localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; interacts genetically with CYC1 and with cytochrome c oxidase assembly factors Gene:COS1(YNL336W)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:COY1(YKL179C)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function Gene:CSH1(YBR161W)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication Gene:DYN1(YKR054C)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:Cytoplasmic heavy chain dynein, microtubule motor protein, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p Gene:FAR10(YLR238W)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate Gene:FUS2(YMR232W)|FD-Score:4.31|P-value:8.00E-6||SGD DESC:Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating Gene:GLO3(YER122C)|FD-Score:-5.54|P-value:1.54E-8||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Gcs1p Gene:HAP4(YKL109W)|FD-Score:3.09|P-value:9.89E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex Gene:HAP5(YOR358W)|FD-Score:-3.11|P-value:9.26E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2/3/4/5 CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex Gene:HEM14(YER014W)|FD-Score:-3.16|P-value:7.94E-4||SGD DESC:Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides Gene:HOS3(YPL116W)|FD-Score:5.91|P-value:1.69E-9||SGD DESC:Trichostatin A-insensitive homodimeric histone deacetylase (HDAC) with specificity in vitro for histones H3, H4, H2A, and H2B; similar to Hda1p, Rpd3p, Hos1p, and Hos2p; deletion results in increased histone acetylation at rDNA repeats Gene:HSM3(YBR272C)|FD-Score:3.73|P-value:9.54E-5||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation Gene:HST1(YOL068C)|FD-Score:3.8|P-value:7.14E-5||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:IBA57(YJR122W)|FD-Score:3.76|P-value:8.40E-5||SGD DESC:Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system Gene:IRA2(YOL081W)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication Gene:ISU2(YOR226C)|FD-Score:3.1|P-value:9.54E-4||SGD DESC:Protein required for synthesis of iron-sulfur proteins; localized to the mitochondrial matrix; performs a scaffolding function in mitochondria during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; isu1 isu2 double mutant is inviable; protein abundance increases in response to DNA replication stress; evolutionarily conserved Gene:KIN2(YLR096W)|FD-Score:-4.16|P-value:1.59E-5||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p Gene:KTR7(YIL085C)|FD-Score:5.25|P-value:7.52E-8||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication Gene:LAP2(YNL045W)|FD-Score:4.98|P-value:3.18E-7||SGD DESC:Leucyl aminopeptidase yscIV (leukotriene A4 hydrolase) with epoxide hydrolase activity, metalloenzyme containing one zinc atom; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:LCB5(YLR260W)|FD-Score:-6.22|P-value:2.55E-10||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:LEM3(YNL323W)|FD-Score:-3.09|P-value:9.95E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MCM16(YPR046W)|FD-Score:-3.16|P-value:7.79E-4||SGD DESC:Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3 Gene:MDM12(YOL009C)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:Mitochondrial outer membrane protein, required for transmission of mitochondria to daughter cells; component of the ERMES complex that links the ER to mitochondria; may influence import and assembly of outer membrane beta-barrel proteins Gene:MFA2(YNL145W)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA1 Gene:MGT1(YDL200C)|FD-Score:-3.48|P-value:2.53E-4||SGD DESC:DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage Gene:MIH1(YMR036C)|FD-Score:4.45|P-value:4.24E-6||SGD DESC:Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25 Gene:MOT2(YER068W)|FD-Score:-3.31|P-value:4.64E-4||SGD DESC:Ubiquitin-protein ligase subunit of the CCR4-NOT complex; with Ubc4p, ubiquitinates nascent polypeptide-associated complex subunits and histone demethyase Jhd2p; CCR4-NOT has roles in transcription regulation, mRNA degradation, and post-transcriptional modifications Gene:MPD2(YOL088C)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p Gene:MRPL4(YLR439W)|FD-Score:-3.77|P-value:8.15E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:MSA2(YKR077W)|FD-Score:5.2|P-value:9.80E-8||SGD DESC:Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation Gene:MSB2(YGR014W)|FD-Score:-4.75|P-value:1.02E-6||SGD DESC:Mucin family member involved in signaling; functions in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; is processed into secreted and cell-associated forms by the aspartyl protease, Yps1p; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate Gene:MSM1(YGR171C)|FD-Score:-7.07|P-value:7.92E-13||SGD DESC:Mitochondrial methionyl-tRNA synthetase (MetRS), functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p Gene:MUD1(YBR119W)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:U1 snRNP A protein, homolog of human U1-A; involved in nuclear mRNA splicing Gene:NFT1(YKR103W)|FD-Score:5.97|P-value:1.17E-9||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds. Gene:PDH1(YPR002W)|FD-Score:-3.41|P-value:3.19E-4||SGD DESC:Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate Gene:PDR11(YIL013C)|FD-Score:4.35|P-value:6.77E-6||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth Gene:PET123(YOR158W)|FD-Score:3.88|P-value:5.28E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator Gene:PEX28(YHR150W)|FD-Score:3.25|P-value:5.83E-4||SGD DESC:Peroxisomal integral membrane peroxin, involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p Gene:PHO85(YPL031C)|FD-Score:-4.8|P-value:8.08E-7||SGD DESC:Cyclin-dependent kinase, with ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle Gene:PIM1(YBL022C)|FD-Score:-3.77|P-value:8.08E-5||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PMD1(YER132C)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions Gene:PMT3(YOR321W)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication Gene:RCK2(YLR248W)|FD-Score:3.88|P-value:5.18E-5||SGD DESC:Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication Gene:RPL19B(YBL027W)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Ribosomal 60S subunit protein L19B; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19B has a paralog, RPL19A, that arose from the whole genome duplication Gene:RPL27A(YHR010W)|FD-Score:-3.57|P-value:1.79E-4||SGD DESC:Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication Gene:SBH1(YER087C-B)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SER33(YIL074C)|FD-Score:-3.41|P-value:3.23E-4||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER33 has a paralog, SER3, that arose from the whole genome duplication Gene:SKI7(YOR076C)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Coupling protein that mediates interactions between the Ski complex and the cytoplasmic exosome during 3'-5' RNA degradation; eRF3-like domain targets nonstop mRNA for degradation; null mutants have superkiller phenotype Gene:SMY1(YKL079W)|FD-Score:4.03|P-value:2.82E-5||SGD DESC:Protein that interacts with Myo2p, proposed to be involved in exocytosis; N-terminal domain is related to the motor domain of kinesins Gene:SNL1(YIL016W)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Ribosome-associated protein proposed to act in protein synthesis and nuclear pore complex biogenesis and maintenance as well as protein folding; has similarity to the mammalian BAG-1 protein Gene:SPO71(YDR104C)|FD-Score:5.11|P-value:1.59E-7||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation, in particular for the proper size of the prospore membrane (PSM); interacts with SPO1; dispensable for both nuclear divisions during meiosis; contains two PH domains Gene:SRC1(YML034W)|FD-Score:-3.16|P-value:7.78E-4||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:STB2(YMR053C)|FD-Score:3.96|P-value:3.75E-5||SGD DESC:Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p Gene:SWC5(YBR231C)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:VHS1(YDR247W)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Cytoplasmic serine/threonine protein kinase; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; homolog of Sks1p Gene:VPS34(YLR240W)|FD-Score:-4.94|P-value:3.94E-7||SGD DESC:Phosphatidylinositol (PI) 3-kinase that synthesizes PI-3-phosphate; forms membrane-associated signal transduction complex with Vps15p to regulate protein sorting; activated by the GTP-bound form of Gpa1p; a fraction is localized, with Vps15p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery Gene:YAR029W(YAR029W_p)|FD-Score:4.04|P-value:2.66E-5||SGD DESC:Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YBL065W(YBL065W_d)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF SEF1/YBL066C; YBL065W is a non-essential gene Gene:YDJ1(YNL064C)|FD-Score:-3.22|P-value:6.42E-4||SGD DESC:Type I HSP40 co-chaperone; involved in regulation of HSP90 and HSP70 functions; critical for determining cell size at Start as a function of growth rate; involved in protein translocation across membranes; member of the DnaJ family Gene:YDR524W-C(YDR524W-C_p)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Putative protein of unknown function; small ORF identified by SAGE; deletion strains are moderately sensitive to the radiomimetic drug bleomycin Gene:YFL032W(YFL032W_d)|FD-Score:-3.2|P-value:6.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene Gene:YFT2(YDR319C_p)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens Gene:YGR035W-A(YGR035W-A_p)|FD-Score:4.12|P-value:1.92E-5||SGD DESC:Putative protein of unknown function Gene:YHL015W-A(YHL015W-A_p)|FD-Score:-3.27|P-value:5.46E-4||SGD DESC:Putative protein of unknown function Gene:YIG1(YPL201C)|FD-Score:-4.02|P-value:2.94E-5||SGD DESC:Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol Gene:YJL064W(YJL064W_d)|FD-Score:3.18|P-value:7.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL065C/DLS1 Gene:YJL169W(YJL169W_d)|FD-Score:3.88|P-value:5.24E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2 Gene:YJR111C(YJR111C_p)|FD-Score:3.84|P-value:6.09E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria Gene:YML082W(YML082W_p)|FD-Score:-4.67|P-value:1.53E-6||SGD DESC:Putative protein predicted to have carbon-sulfur lyase activity; transcriptionally regulated by Upc2p via an upstream sterol response element; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; YML082W is not an essential gene Gene:YML083C(YML083C_p)|FD-Score:3.2|P-value:6.91E-4||SGD DESC:Protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions Gene:YMR119W-A(YMR119W-A_d)|FD-Score:-3.18|P-value:7.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNK1(YKL067W)|FD-Score:5.87|P-value:2.22E-9||SGD DESC:Nucleoside diphosphate kinase; catalyzes the transfer of gamma phosphates from nucleoside triphosphates, usually ATP, to nucleoside diphosphates by a mechanism that involves formation of an autophosphorylated enzyme intermediate; protein abundance increases in response to DNA replication stress Gene:YNL195C(YNL195C_p)|FD-Score:5.16|P-value:1.25E-7||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YNR048W(YNR048W)|FD-Score:3.13|P-value:8.66E-4||SGD DESC:Protein that interacts with phospholipid translocase (flippase) Dnf3p; YNR048W has a paralog, CDC50, that arose from the whole genome duplication Gene:YOR059C(YOR059C_p)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Lipid particle protein of unknown function; contains a putative lipase serine active site; induced by transcription factor Rpn4p; protein abundance increases in response to DNA replication stress Gene:YOR365C(YOR365C_p)|FD-Score:6.99|P-value:1.42E-12||SGD DESC:Putative protein of unknown function; not an essential protein; YOR365C has a paralog, FLC2, that arose from the whole genome duplication Gene:YPL162C(YPL162C_p)|FD-Score:-3.1|P-value:9.52E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology Gene:YPL197C(YPL197C_d)|FD-Score:3.82|P-value:6.76E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the ribosomal gene RPB7B Gene:YRF1-6(YNL339C)|FD-Score:3.79|P-value:7.39E-5||SGD DESC:Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p Gene:YRO2(YBR054W)|FD-Score:4.7|P-value:1.29E-6||SGD DESC:Protein of unknown function with similarity to archaeal rhodopsins; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; transcriptionally regulated by Haa1p; YRO2 has a paralog, MRH1, that arose from the whole genome duplication Gene:ADE5,7(YGL234W)|FD-Score:3.85|P-value:5.94E-5||SGD DESC:Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities Gene:AIM37(YNL100W)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Mitochondrial inner membrane protein; component of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:APE3(YBR286W)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Vacuolar aminopeptidase Y, processed to mature form by Prb1p Gene:ARC1(YGL105W)|FD-Score:-4.24|P-value:1.14E-5||SGD DESC:Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress Gene:ATG13(YPR185W)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Regulatory subunit of the Atg1p signaling complex; stimulates Atg1p kinase activity; required for vesicle formation during autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; involved in Atg9p, Atg23p, and Atg27p cycling Gene:ATG17(YLR423C)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells Gene:CBP1(YJL209W)|FD-Score:4.63|P-value:1.79E-6||SGD DESC:Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress Gene:CCW14(YLR390W-A)|FD-Score:3.18|P-value:7.27E-4||SGD DESC:Covalently linked cell wall glycoprotein, present in the inner layer of the cell wall Gene:COA4(YLR218C)|FD-Score:3.29|P-value:4.97E-4||SGD DESC:Twin Cx(9)C protein involved in cytochrome c oxidase assembly or stability; localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; interacts genetically with CYC1 and with cytochrome c oxidase assembly factors Gene:COS1(YNL336W)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:COY1(YKL179C)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function Gene:CSH1(YBR161W)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication Gene:DYN1(YKR054C)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:Cytoplasmic heavy chain dynein, microtubule motor protein, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p Gene:FAR10(YLR238W)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate Gene:FUS2(YMR232W)|FD-Score:4.31|P-value:8.00E-6||SGD DESC:Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating Gene:GLO3(YER122C)|FD-Score:-5.54|P-value:1.54E-8||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Gcs1p Gene:HAP4(YKL109W)|FD-Score:3.09|P-value:9.89E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex Gene:HAP5(YOR358W)|FD-Score:-3.11|P-value:9.26E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2/3/4/5 CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex Gene:HEM14(YER014W)|FD-Score:-3.16|P-value:7.94E-4||SGD DESC:Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides Gene:HOS3(YPL116W)|FD-Score:5.91|P-value:1.69E-9||SGD DESC:Trichostatin A-insensitive homodimeric histone deacetylase (HDAC) with specificity in vitro for histones H3, H4, H2A, and H2B; similar to Hda1p, Rpd3p, Hos1p, and Hos2p; deletion results in increased histone acetylation at rDNA repeats Gene:HSM3(YBR272C)|FD-Score:3.73|P-value:9.54E-5||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation Gene:HST1(YOL068C)|FD-Score:3.8|P-value:7.14E-5||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:IBA57(YJR122W)|FD-Score:3.76|P-value:8.40E-5||SGD DESC:Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system Gene:IRA2(YOL081W)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication Gene:ISU2(YOR226C)|FD-Score:3.1|P-value:9.54E-4||SGD DESC:Protein required for synthesis of iron-sulfur proteins; localized to the mitochondrial matrix; performs a scaffolding function in mitochondria during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; isu1 isu2 double mutant is inviable; protein abundance increases in response to DNA replication stress; evolutionarily conserved Gene:KIN2(YLR096W)|FD-Score:-4.16|P-value:1.59E-5||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p Gene:KTR7(YIL085C)|FD-Score:5.25|P-value:7.52E-8||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication Gene:LAP2(YNL045W)|FD-Score:4.98|P-value:3.18E-7||SGD DESC:Leucyl aminopeptidase yscIV (leukotriene A4 hydrolase) with epoxide hydrolase activity, metalloenzyme containing one zinc atom; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:LCB5(YLR260W)|FD-Score:-6.22|P-value:2.55E-10||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:LEM3(YNL323W)|FD-Score:-3.09|P-value:9.95E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MCM16(YPR046W)|FD-Score:-3.16|P-value:7.79E-4||SGD DESC:Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3 Gene:MDM12(YOL009C)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:Mitochondrial outer membrane protein, required for transmission of mitochondria to daughter cells; component of the ERMES complex that links the ER to mitochondria; may influence import and assembly of outer membrane beta-barrel proteins Gene:MFA2(YNL145W)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA1 Gene:MGT1(YDL200C)|FD-Score:-3.48|P-value:2.53E-4||SGD DESC:DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage Gene:MIH1(YMR036C)|FD-Score:4.45|P-value:4.24E-6||SGD DESC:Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25 Gene:MOT2(YER068W)|FD-Score:-3.31|P-value:4.64E-4||SGD DESC:Ubiquitin-protein ligase subunit of the CCR4-NOT complex; with Ubc4p, ubiquitinates nascent polypeptide-associated complex subunits and histone demethyase Jhd2p; CCR4-NOT has roles in transcription regulation, mRNA degradation, and post-transcriptional modifications Gene:MPD2(YOL088C)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p Gene:MRPL4(YLR439W)|FD-Score:-3.77|P-value:8.15E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:MSA2(YKR077W)|FD-Score:5.2|P-value:9.80E-8||SGD DESC:Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation Gene:MSB2(YGR014W)|FD-Score:-4.75|P-value:1.02E-6||SGD DESC:Mucin family member involved in signaling; functions in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; is processed into secreted and cell-associated forms by the aspartyl protease, Yps1p; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate Gene:MSM1(YGR171C)|FD-Score:-7.07|P-value:7.92E-13||SGD DESC:Mitochondrial methionyl-tRNA synthetase (MetRS), functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p Gene:MUD1(YBR119W)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:U1 snRNP A protein, homolog of human U1-A; involved in nuclear mRNA splicing Gene:NFT1(YKR103W)|FD-Score:5.97|P-value:1.17E-9||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds. Gene:PDH1(YPR002W)|FD-Score:-3.41|P-value:3.19E-4||SGD DESC:Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate Gene:PDR11(YIL013C)|FD-Score:4.35|P-value:6.77E-6||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth Gene:PET123(YOR158W)|FD-Score:3.88|P-value:5.28E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator Gene:PEX28(YHR150W)|FD-Score:3.25|P-value:5.83E-4||SGD DESC:Peroxisomal integral membrane peroxin, involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p Gene:PHO85(YPL031C)|FD-Score:-4.8|P-value:8.08E-7||SGD DESC:Cyclin-dependent kinase, with ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle Gene:PIM1(YBL022C)|FD-Score:-3.77|P-value:8.08E-5||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PMD1(YER132C)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions Gene:PMT3(YOR321W)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication Gene:RCK2(YLR248W)|FD-Score:3.88|P-value:5.18E-5||SGD DESC:Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication Gene:RPL19B(YBL027W)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Ribosomal 60S subunit protein L19B; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19B has a paralog, RPL19A, that arose from the whole genome duplication Gene:RPL27A(YHR010W)|FD-Score:-3.57|P-value:1.79E-4||SGD DESC:Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication Gene:SBH1(YER087C-B)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SER33(YIL074C)|FD-Score:-3.41|P-value:3.23E-4||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER33 has a paralog, SER3, that arose from the whole genome duplication Gene:SKI7(YOR076C)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Coupling protein that mediates interactions between the Ski complex and the cytoplasmic exosome during 3'-5' RNA degradation; eRF3-like domain targets nonstop mRNA for degradation; null mutants have superkiller phenotype Gene:SMY1(YKL079W)|FD-Score:4.03|P-value:2.82E-5||SGD DESC:Protein that interacts with Myo2p, proposed to be involved in exocytosis; N-terminal domain is related to the motor domain of kinesins Gene:SNL1(YIL016W)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Ribosome-associated protein proposed to act in protein synthesis and nuclear pore complex biogenesis and maintenance as well as protein folding; has similarity to the mammalian BAG-1 protein Gene:SPO71(YDR104C)|FD-Score:5.11|P-value:1.59E-7||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation, in particular for the proper size of the prospore membrane (PSM); interacts with SPO1; dispensable for both nuclear divisions during meiosis; contains two PH domains Gene:SRC1(YML034W)|FD-Score:-3.16|P-value:7.78E-4||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:STB2(YMR053C)|FD-Score:3.96|P-value:3.75E-5||SGD DESC:Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p Gene:SWC5(YBR231C)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:VHS1(YDR247W)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Cytoplasmic serine/threonine protein kinase; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; homolog of Sks1p Gene:VPS34(YLR240W)|FD-Score:-4.94|P-value:3.94E-7||SGD DESC:Phosphatidylinositol (PI) 3-kinase that synthesizes PI-3-phosphate; forms membrane-associated signal transduction complex with Vps15p to regulate protein sorting; activated by the GTP-bound form of Gpa1p; a fraction is localized, with Vps15p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery Gene:YAR029W(YAR029W_p)|FD-Score:4.04|P-value:2.66E-5||SGD DESC:Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YBL065W(YBL065W_d)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF SEF1/YBL066C; YBL065W is a non-essential gene Gene:YDJ1(YNL064C)|FD-Score:-3.22|P-value:6.42E-4||SGD DESC:Type I HSP40 co-chaperone; involved in regulation of HSP90 and HSP70 functions; critical for determining cell size at Start as a function of growth rate; involved in protein translocation across membranes; member of the DnaJ family Gene:YDR524W-C(YDR524W-C_p)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Putative protein of unknown function; small ORF identified by SAGE; deletion strains are moderately sensitive to the radiomimetic drug bleomycin Gene:YFL032W(YFL032W_d)|FD-Score:-3.2|P-value:6.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene Gene:YFT2(YDR319C_p)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens Gene:YGR035W-A(YGR035W-A_p)|FD-Score:4.12|P-value:1.92E-5||SGD DESC:Putative protein of unknown function Gene:YHL015W-A(YHL015W-A_p)|FD-Score:-3.27|P-value:5.46E-4||SGD DESC:Putative protein of unknown function Gene:YIG1(YPL201C)|FD-Score:-4.02|P-value:2.94E-5||SGD DESC:Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol Gene:YJL064W(YJL064W_d)|FD-Score:3.18|P-value:7.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL065C/DLS1 Gene:YJL169W(YJL169W_d)|FD-Score:3.88|P-value:5.24E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2 Gene:YJR111C(YJR111C_p)|FD-Score:3.84|P-value:6.09E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria Gene:YML082W(YML082W_p)|FD-Score:-4.67|P-value:1.53E-6||SGD DESC:Putative protein predicted to have carbon-sulfur lyase activity; transcriptionally regulated by Upc2p via an upstream sterol response element; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; YML082W is not an essential gene Gene:YML083C(YML083C_p)|FD-Score:3.2|P-value:6.91E-4||SGD DESC:Protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions Gene:YMR119W-A(YMR119W-A_d)|FD-Score:-3.18|P-value:7.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNK1(YKL067W)|FD-Score:5.87|P-value:2.22E-9||SGD DESC:Nucleoside diphosphate kinase; catalyzes the transfer of gamma phosphates from nucleoside triphosphates, usually ATP, to nucleoside diphosphates by a mechanism that involves formation of an autophosphorylated enzyme intermediate; protein abundance increases in response to DNA replication stress Gene:YNL195C(YNL195C_p)|FD-Score:5.16|P-value:1.25E-7||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YNR048W(YNR048W)|FD-Score:3.13|P-value:8.66E-4||SGD DESC:Protein that interacts with phospholipid translocase (flippase) Dnf3p; YNR048W has a paralog, CDC50, that arose from the whole genome duplication Gene:YOR059C(YOR059C_p)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Lipid particle protein of unknown function; contains a putative lipase serine active site; induced by transcription factor Rpn4p; protein abundance increases in response to DNA replication stress Gene:YOR365C(YOR365C_p)|FD-Score:6.99|P-value:1.42E-12||SGD DESC:Putative protein of unknown function; not an essential protein; YOR365C has a paralog, FLC2, that arose from the whole genome duplication Gene:YPL162C(YPL162C_p)|FD-Score:-3.1|P-value:9.52E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology Gene:YPL197C(YPL197C_d)|FD-Score:3.82|P-value:6.76E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the ribosomal gene RPB7B Gene:YRF1-6(YNL339C)|FD-Score:3.79|P-value:7.39E-5||SGD DESC:Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p Gene:YRO2(YBR054W)|FD-Score:4.7|P-value:1.29E-6||SGD DESC:Protein of unknown function with similarity to archaeal rhodopsins; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; transcriptionally regulated by Haa1p; YRO2 has a paralog, MRH1, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR060C6.019.47E-101.87ORC2Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p
YFR029W4.141.72E-50.06PTR3Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes
YNL207W4.082.28E-50.37RIO2Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p
YHR118C3.711.03E-40.15ORC6Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p
YIL068C3.561.84E-40.13SEC6Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p
YPL251W_d3.432.99E-40.08YPL251W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C
YDR172W3.353.97E-40.02SUP35Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype
YDR243C3.334.34E-40.11PRP28RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site
YER038C3.226.43E-40.20KRE29Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance
YIL106W3.010.001290.15MOB1Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
YOL038W2.870.002070.08PRE6Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
YGR091W2.790.002620.00PRP31Splicing factor, component of the U4/U6-U5 snRNP complex
YHR042W2.790.002660.03NCP1NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p
YER036C2.760.002920.13ARB1ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p
YGR082W2.630.004330.01TOM20Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOR365C_p6.991.42E-12YOR365C_pPutative protein of unknown function; not an essential protein; YOR365C has a paralog, FLC2, that arose from the whole genome duplication
YKR103W5.971.17E-9NFT1Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds.
YPL116W5.911.69E-9HOS3Trichostatin A-insensitive homodimeric histone deacetylase (HDAC) with specificity in vitro for histones H3, H4, H2A, and H2B; similar to Hda1p, Rpd3p, Hos1p, and Hos2p; deletion results in increased histone acetylation at rDNA repeats
YKL067W5.872.22E-9YNK1Nucleoside diphosphate kinase; catalyzes the transfer of gamma phosphates from nucleoside triphosphates, usually ATP, to nucleoside diphosphates by a mechanism that involves formation of an autophosphorylated enzyme intermediate; protein abundance increases in response to DNA replication stress
YIL085C5.257.52E-8KTR7Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication
YKR077W5.209.80E-8MSA2Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation
YNL195C_p5.161.25E-7YNL195C_pPutative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YDR104C5.111.59E-7SPO71Meiosis-specific protein of unknown function, required for spore wall formation during sporulation, in particular for the proper size of the prospore membrane (PSM); interacts with SPO1; dispensable for both nuclear divisions during meiosis; contains two PH domains
YNL045W4.983.18E-7LAP2Leucyl aminopeptidase yscIV (leukotriene A4 hydrolase) with epoxide hydrolase activity, metalloenzyme containing one zinc atom; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
YBR054W4.701.29E-6YRO2Protein of unknown function with similarity to archaeal rhodopsins; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; transcriptionally regulated by Haa1p; YRO2 has a paralog, MRH1, that arose from the whole genome duplication
YJL209W4.631.79E-6CBP1Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress
YMR036C4.454.24E-6MIH1Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25
YIL013C4.356.77E-6PDR11ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth
YMR232W4.318.00E-6FUS2Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating
YGR035W-A_p4.121.92E-5YGR035W-A_pPutative protein of unknown function

GO enrichment analysis for SGTC_2919
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0714.19E-8SGTC_29187977171 22.1 μMChembridge (Drug-like library)22364830.246377
0.0543.75E-5SGTC_24325630864 12.2 μMMiscellaneous57149700.0649351
0.0501.32E-4SGTC_31339127736 49.5 μMChembridge (Drug-like library)272434200.142857
0.0464.00E-4SGTC_185k048-0037 25.5 μMChemDiv (Drug-like library)68275890.178082RSC & ERG11
0.0464.10E-4SGTC_5643555-0129 330.0 μMChemDiv (Drug-like library)5632600.136986
0.0446.47E-4SGTC_15773(2'-chlorophenyl)-7-ethoxycoumarin 66.5 μMTimTec (Pure natural product library)6890070.16
0.0410.00174SGTC_31849108758 49.5 μMChembridge (Drug-like library)9028850.161765
0.0410.00184SGTC_24835729050 27.2 μMMiscellaneous28651800.189873
0.0400.00212SGTC_1557sumaresinolic acid 42.3 μMTimTec (Pure natural product library)99822680.0888889
0.0390.00251SGTC_287k060-0039 29.6 μMChemDiv (Drug-like library)58619740.148649
0.0380.00317SGTC_1083didemethylchlorpromazine sulfoxide 707.4 nMTimTec (Natural product library)3987880.0921053redox potentiating
0.0380.00358SGTC_12710832-2808 169.0 μMChemDiv (Drug-like library)36637940.0857143
0.0380.00367SGTC_32159129942 49.5 μMChembridge (Drug-like library)176829440.121951
0.0380.00371SGTC_23417987486 176.7 μMChembridge (Fragment library)12586860.208955
0.0380.00384SGTC_11760848-0131 83.3 μMChemDiv (Drug-like library)67690400.0963855Golgi

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2471546695624.79 μM0.415094724419Miscellaneous274.699043.57713mitochondrial processes
SGTC_2449581298245.77 μM0.322581776185Miscellaneous292.6895033.78214
SGTC_245958111579.22 μM0.3220342870684Miscellaneous258.2444433.11814mitochondrial processes
SGTC_2481547894845.16 μM0.32203477792Miscellaneous270.279962.89614PDR1
SGTC_2001402350257.66 μM0.314815108941Chembridge (Fragment library)195.602441.5402mitochondrial processes
SGTC_22667929956200 μM0.3114752967153Chembridge (Fragment library)238.670240.76912
SGTC_2235salicylic acid500 μM0.306122338Miscellaneous138.120741.21723
SGTC_3021094-006318.9 μM0.281695440289ChemDiv (Drug-like library)374.188782.47624
SGTC_23369001819198.65 μM0.2758622989602Chembridge (Fragment library)209.629021.88902heme biosynthesis & mitochondrial translocase
SGTC_2477596768625.23 μM0.269841776118Miscellaneous270.279962.89614