7974240

ethyl 4-[3-(4-chlorophenyl)-3-oxopropyl]piperazine-1-carboxylate dihydrochloride

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2921
Screen concentration 10.7 μM
Source Chembridge (Drug-like library)
PubChem CID 2979074
SMILES CCOC(=O)N1CCN(CC1)CCC(=O)C2=CC=C(C=C2)Cl.Cl.Cl
Standardized SMILES CCOC(=O)N1CCN(CCC(=O)c2ccc(Cl)cc2)CC1
Molecular weight 397.7244
ALogP 3.46
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 18.83
% growth inhibition (Hom. pool) 6.57


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2979074
Download HIP data (tab-delimited text)  (excel)
Gene:ACC1(YNR016C)|FD-Score:4.83|P-value:6.88E-7|Clearance:0.06||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ARC19(YKL013C)|FD-Score:3.2|P-value:6.81E-4|Clearance:0.19||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARC35(YNR035C)|FD-Score:-4.67|P-value:1.48E-6|Clearance:0||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin Gene:CWC2(YDL209C)|FD-Score:4.49|P-value:3.60E-6|Clearance:0.26||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:DED1(YOR204W)|FD-Score:5.21|P-value:9.62E-8|Clearance:0.09||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:DUT1(YBR252W)|FD-Score:-3.34|P-value:4.21E-4|Clearance:0||SGD DESC:deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate Gene:ESS1(YJR017C)|FD-Score:3.57|P-value:1.78E-4|Clearance:0.01||SGD DESC:Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated serine and threonine residues N-terminal to proline; regulates phosphorylation of the RNAP II large subunit (Rpo21p) C-terminal domain (CTD); associates with phospho-Ser5 form of RNAP II in vivo; regulates phosphorylation of Ser7 within CTD; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription and trimethylation of histone H3 Gene:GPI14(YJR013W)|FD-Score:3.69|P-value:1.13E-4|Clearance:0.01||SGD DESC:Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M Gene:GPN2(YOR262W)|FD-Score:8.33|P-value:3.88E-17|Clearance:1.48||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion Gene:GPN3(YLR243W_p)|FD-Score:4.77|P-value:9.27E-7|Clearance:0.11||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p Gene:HEM4(YOR278W)|FD-Score:-4.01|P-value:3.04E-5|Clearance:0||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:IRR1(YIL026C)|FD-Score:3.56|P-value:1.84E-4|Clearance:0.04||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:MIA40(YKL195W)|FD-Score:5.33|P-value:4.87E-8|Clearance:0.12||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:MPS3(YJL019W)|FD-Score:3.53|P-value:2.11E-4|Clearance:0.13||SGD DESC:Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p Gene:NMD3(YHR170W)|FD-Score:-3.39|P-value:3.44E-4|Clearance:0||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NUP192(YJL039C)|FD-Score:4.22|P-value:1.20E-5|Clearance:0.1||SGD DESC:Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205 Gene:NUS1(YDL193W)|FD-Score:4.66|P-value:1.57E-6|Clearance:0.17||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PAM16(YJL104W)|FD-Score:3.68|P-value:1.16E-4|Clearance:0.11||SGD DESC:Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); forms a 1:1 subcomplex with Pam18p and inhibits its cochaperone activity; contains a J-like domain Gene:PIK1(YNL267W)|FD-Score:-3.8|P-value:7.22E-5|Clearance:0||SGD DESC:Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton Gene:PRE6(YOL038W)|FD-Score:3.91|P-value:4.53E-5|Clearance:0.02||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PRP2(YNR011C)|FD-Score:3.4|P-value:3.38E-4|Clearance:0.06||SGD DESC:RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16 Gene:RBA50(YDR527W)|FD-Score:30.1|P-value:2.27E-199|Clearance:14.57||SGD DESC:Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 Gene:RET1(YOR207C)|FD-Score:15.5|P-value:1.06E-54|Clearance:7.2||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPB8(YOR224C)|FD-Score:-5.69|P-value:6.41E-9|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPC11(YDR045C)|FD-Score:-5.25|P-value:7.41E-8|Clearance:0||SGD DESC:RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS Gene:RPC53(YDL150W)|FD-Score:4.12|P-value:1.86E-5|Clearance:0.12||SGD DESC:RNA polymerase III subunit C53 Gene:RPL15A(YLR029C)|FD-Score:3.34|P-value:4.15E-4|Clearance:0.14||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPN11(YFR004W)|FD-Score:4|P-value:3.11E-5|Clearance:0.09||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:RPN3(YER021W)|FD-Score:3.57|P-value:1.82E-4|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RPO26(YPR187W)|FD-Score:3.9|P-value:4.86E-5|Clearance:0.21||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RPP1(YHR062C)|FD-Score:6.85|P-value:3.62E-12|Clearance:1.52||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RTP1(YMR185W_p)|FD-Score:4.92|P-value:4.23E-7|Clearance:0.1||SGD DESC:Protein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene Gene:SEC26(YDR238C)|FD-Score:-3.26|P-value:5.63E-4|Clearance:0||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SRP102(YKL154W)|FD-Score:-3.43|P-value:2.99E-4|Clearance:0||SGD DESC:Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane Gene:SUP45(YBR143C)|FD-Score:-5.73|P-value:4.89E-9|Clearance:0||SGD DESC:Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p Gene:TAD3(YLR316C)|FD-Score:-5.34|P-value:4.74E-8|Clearance:0||SGD DESC:Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad2p that converts adenosine to inosine at the wobble position of several tRNAs Gene:YBR124W(YBR124W_d)|FD-Score:-3.16|P-value:7.81E-4|Clearance:0||SGD DESC:Putative protein of unknown function Gene:YCG1(YDR325W)|FD-Score:-3.75|P-value:8.83E-5|Clearance:0||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YKL111C(YKL111C_d)|FD-Score:5.12|P-value:1.57E-7|Clearance:0.19||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene ABF1 Gene:YNL150W(YNL150W_d)|FD-Score:-3.31|P-value:4.63E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking Gene:ACC1(YNR016C)|FD-Score:4.83|P-value:6.88E-7|Clearance:0.06||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ARC19(YKL013C)|FD-Score:3.2|P-value:6.81E-4|Clearance:0.19||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARC35(YNR035C)|FD-Score:-4.67|P-value:1.48E-6|Clearance:0||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin Gene:CWC2(YDL209C)|FD-Score:4.49|P-value:3.60E-6|Clearance:0.26||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:DED1(YOR204W)|FD-Score:5.21|P-value:9.62E-8|Clearance:0.09||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:DUT1(YBR252W)|FD-Score:-3.34|P-value:4.21E-4|Clearance:0||SGD DESC:deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate Gene:ESS1(YJR017C)|FD-Score:3.57|P-value:1.78E-4|Clearance:0.01||SGD DESC:Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated serine and threonine residues N-terminal to proline; regulates phosphorylation of the RNAP II large subunit (Rpo21p) C-terminal domain (CTD); associates with phospho-Ser5 form of RNAP II in vivo; regulates phosphorylation of Ser7 within CTD; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription and trimethylation of histone H3 Gene:GPI14(YJR013W)|FD-Score:3.69|P-value:1.13E-4|Clearance:0.01||SGD DESC:Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M Gene:GPN2(YOR262W)|FD-Score:8.33|P-value:3.88E-17|Clearance:1.48||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion Gene:GPN3(YLR243W_p)|FD-Score:4.77|P-value:9.27E-7|Clearance:0.11||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p Gene:HEM4(YOR278W)|FD-Score:-4.01|P-value:3.04E-5|Clearance:0||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:IRR1(YIL026C)|FD-Score:3.56|P-value:1.84E-4|Clearance:0.04||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:MIA40(YKL195W)|FD-Score:5.33|P-value:4.87E-8|Clearance:0.12||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:MPS3(YJL019W)|FD-Score:3.53|P-value:2.11E-4|Clearance:0.13||SGD DESC:Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p Gene:NMD3(YHR170W)|FD-Score:-3.39|P-value:3.44E-4|Clearance:0||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NUP192(YJL039C)|FD-Score:4.22|P-value:1.20E-5|Clearance:0.1||SGD DESC:Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205 Gene:NUS1(YDL193W)|FD-Score:4.66|P-value:1.57E-6|Clearance:0.17||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PAM16(YJL104W)|FD-Score:3.68|P-value:1.16E-4|Clearance:0.11||SGD DESC:Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); forms a 1:1 subcomplex with Pam18p and inhibits its cochaperone activity; contains a J-like domain Gene:PIK1(YNL267W)|FD-Score:-3.8|P-value:7.22E-5|Clearance:0||SGD DESC:Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton Gene:PRE6(YOL038W)|FD-Score:3.91|P-value:4.53E-5|Clearance:0.02||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PRP2(YNR011C)|FD-Score:3.4|P-value:3.38E-4|Clearance:0.06||SGD DESC:RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16 Gene:RBA50(YDR527W)|FD-Score:30.1|P-value:2.27E-199|Clearance:14.57||SGD DESC:Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 Gene:RET1(YOR207C)|FD-Score:15.5|P-value:1.06E-54|Clearance:7.2||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPB8(YOR224C)|FD-Score:-5.69|P-value:6.41E-9|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPC11(YDR045C)|FD-Score:-5.25|P-value:7.41E-8|Clearance:0||SGD DESC:RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS Gene:RPC53(YDL150W)|FD-Score:4.12|P-value:1.86E-5|Clearance:0.12||SGD DESC:RNA polymerase III subunit C53 Gene:RPL15A(YLR029C)|FD-Score:3.34|P-value:4.15E-4|Clearance:0.14||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPN11(YFR004W)|FD-Score:4|P-value:3.11E-5|Clearance:0.09||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:RPN3(YER021W)|FD-Score:3.57|P-value:1.82E-4|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RPO26(YPR187W)|FD-Score:3.9|P-value:4.86E-5|Clearance:0.21||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RPP1(YHR062C)|FD-Score:6.85|P-value:3.62E-12|Clearance:1.52||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RTP1(YMR185W_p)|FD-Score:4.92|P-value:4.23E-7|Clearance:0.1||SGD DESC:Protein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene Gene:SEC26(YDR238C)|FD-Score:-3.26|P-value:5.63E-4|Clearance:0||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SRP102(YKL154W)|FD-Score:-3.43|P-value:2.99E-4|Clearance:0||SGD DESC:Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane Gene:SUP45(YBR143C)|FD-Score:-5.73|P-value:4.89E-9|Clearance:0||SGD DESC:Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p Gene:TAD3(YLR316C)|FD-Score:-5.34|P-value:4.74E-8|Clearance:0||SGD DESC:Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad2p that converts adenosine to inosine at the wobble position of several tRNAs Gene:YBR124W(YBR124W_d)|FD-Score:-3.16|P-value:7.81E-4|Clearance:0||SGD DESC:Putative protein of unknown function Gene:YCG1(YDR325W)|FD-Score:-3.75|P-value:8.83E-5|Clearance:0||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YKL111C(YKL111C_d)|FD-Score:5.12|P-value:1.57E-7|Clearance:0.19||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene ABF1 Gene:YNL150W(YNL150W_d)|FD-Score:-3.31|P-value:4.63E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2979074
Download HOP data (tab-delimited text)  (excel)
Gene:ABZ1(YNR033W)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Para-aminobenzoate (PABA) synthase; has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis; protein abundance increases in response to DNA replication stress Gene:AIM43(YPL099C)|FD-Score:5.5|P-value:1.88E-8||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ALD6(YPL061W)|FD-Score:4.7|P-value:1.29E-6||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:ALF1(YNL148C)|FD-Score:7.91|P-value:1.32E-15||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ARO1(YDR127W)|FD-Score:4.46|P-value:4.16E-6||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:BLM10(YFL007W)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Proteasome activator; binds the core proteasome and stimulates proteasome-mediated protein degradation by inducing gate opening; required for resistance to bleomycin, may be involved in protecting against oxidative damage; similar to mammalian PA200 Gene:BRO1(YPL084W)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes Gene:BUD19(YJL188C_d)|FD-Score:-3.62|P-value:1.50E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:BUD27(YFL023W)|FD-Score:5.03|P-value:2.44E-7||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:CHO1(YER026C)|FD-Score:4.01|P-value:3.10E-5||SGD DESC:Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline Gene:CHS7(YHR142W)|FD-Score:3.18|P-value:7.41E-4||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:CIN1(YOR349W)|FD-Score:3.22|P-value:6.41E-4||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:COX6(YHR051W)|FD-Score:4.73|P-value:1.13E-6||SGD DESC:Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels Gene:CPR5(YDR304C)|FD-Score:-3.66|P-value:1.25E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER Gene:DAL81(YIR023W)|FD-Score:8.76|P-value:1.02E-18||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DBF2(YGR092W)|FD-Score:3.98|P-value:3.39E-5||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DSE2(YHR143W)|FD-Score:7.35|P-value:1.00E-13||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:DUG2(YBR281C)|FD-Score:3.85|P-value:6.02E-5||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:ECM38(YLR299W)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Gamma-glutamyltranspeptidase, major glutathione-degrading enzyme; involved in detoxification of electrophilic xenobiotics; expression induced mainly by nitrogen starvation Gene:EKI1(YDR147W)|FD-Score:3.94|P-value:4.00E-5||SGD DESC:Ethanolamine kinase; primarily responsible for phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; exhibits some choline kinase activity, thus contributing to phosphatidylcholine synthesis via the CDP-choline pathway; EKI1 has a paralog, CKI1, that arose from the whole genome duplication Gene:ELF1(YKL160W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Transcription elongation factor that contains a conserved zinc finger domain; implicated in the maintenance of proper chromatin structure in actively transcribed regions; deletion inhibits Brome mosaic virus (BMV) gene expression Gene:EXO5(YBR163W)|FD-Score:4.94|P-value:3.83E-7||SGD DESC:Mitochondrial 5'-3' exonuclease and sliding exonuclease, required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2 Gene:GBP2(YCL011C)|FD-Score:-3.17|P-value:7.65E-4||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; also binds single-stranded telomeric repeat sequence in vitro; GBP2 has a paralog, HRB1, that arose from the whole genome duplication Gene:GCV1(YDR019C)|FD-Score:-3.31|P-value:4.66E-4||SGD DESC:T subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm Gene:GLE2(YER107C)|FD-Score:11.6|P-value:1.20E-31||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GOS1(YHL031C)|FD-Score:7.61|P-value:1.32E-14||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:HFI1(YPL254W)|FD-Score:3.99|P-value:3.28E-5||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:HHY1(YEL059W_d)|FD-Score:-3.95|P-value:3.87E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; mutant is hypersensitive to hygromycin B indicative of defects in vacuolar trafficking Gene:HMT1(YBR034C)|FD-Score:-4.02|P-value:2.97E-5||SGD DESC:Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region. Gene:HOM6(YJR139C)|FD-Score:5.05|P-value:2.16E-7||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HRT3(YLR097C)|FD-Score:3.25|P-value:5.82E-4||SGD DESC:Putative SCF-ubiquitin ligase F-box protein, based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies Gene:HTZ1(YOL012C)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin Gene:HXK2(YGL253W)|FD-Score:-6.75|P-value:7.20E-12||SGD DESC:Hexokinase isoenzyme 2; catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene; phosphorylation/dephosphorylation at serine-14 by protein kinase Snf1p and protein phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; HXK2 has a paralog, HXK1, that arose from the whole genome duplication Gene:HYR1(YIR037W)|FD-Score:4.23|P-value:1.18E-5||SGD DESC:Thiol peroxidase that functions as a hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce a redox signal to the Yap1p transcription factor Gene:ICS2(YBR157C)|FD-Score:3.2|P-value:6.83E-4||SGD DESC:Protein of unknown function; null mutation does not confer any obvious defects in growth, spore germination, viability, or carbohydrate utilization Gene:IFM1(YOL023W)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Mitochondrial translation initiation factor 2 Gene:ILV1(YER086W)|FD-Score:4.85|P-value:6.03E-7||SGD DESC:Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation Gene:IMG2(YCR071C)|FD-Score:3.72|P-value:9.99E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:INP1(YMR204C)|FD-Score:-3.94|P-value:4.15E-5||SGD DESC:Peripheral membrane protein of peroxisomes involved in peroxisomal inheritance; recruitment to peroxisomes is mediated by interaction with Pex3p at the peroxisomal membrane Gene:IRC11(YOR013W_d)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Dubious opening reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YOR012C; null mutant displays increased levels of spontaneous Rad52 foci Gene:IRC21(YMR073C)|FD-Score:4.18|P-value:1.46E-5||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:KEL3(YPL263C)|FD-Score:-3.16|P-value:7.99E-4||SGD DESC:Cytoplasmic protein of unknown function Gene:LAP3(YNL239W)|FD-Score:6.38|P-value:9.07E-11||SGD DESC:Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH Gene:LCB4(YOR171C)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes Gene:MF(ALPHA)1(YPL187W)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Mating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)2, although MF(ALPHA)1 produces most alpha-factor Gene:MOH1(YBL049W)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:MPC54(YOR177C)|FD-Score:4.67|P-value:1.47E-6||SGD DESC:Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate Gene:MRP51(YPL118W)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MRPL4(YLR439W)|FD-Score:3.24|P-value:6.03E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:MSB2(YGR014W)|FD-Score:-4.72|P-value:1.16E-6||SGD DESC:Mucin family member involved in signaling; functions in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; is processed into secreted and cell-associated forms by the aspartyl protease, Yps1p; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate Gene:MSI1(YBR195C)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription; Msi1p localizes to both nucleus and cytoplasm and has an independent role as a negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase Gene:MSM1(YGR171C)|FD-Score:11|P-value:2.69E-28||SGD DESC:Mitochondrial methionyl-tRNA synthetase (MetRS), functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p Gene:MTG1(YMR097C)|FD-Score:5.98|P-value:1.11E-9||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:MUD2(YKL074C)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65 Gene:NCS6(YGL211W)|FD-Score:4.62|P-value:1.92E-6||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:NIP100(YPL174C)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) Gene:NMA2(YGR010W)|FD-Score:-4.18|P-value:1.47E-5||SGD DESC:Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in de novo and salvage synthesis of NAD(+); NMA2 has a paralog, NMA1, that arose from the whole genome duplication Gene:NUP2(YLR335W)|FD-Score:3.89|P-value:4.92E-5||SGD DESC:Nucleoporin involved in nucleocytoplasmic transport, binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization Gene:OMA1(YKR087C)|FD-Score:5.41|P-value:3.08E-8||SGD DESC:Metalloendopeptidase of the mitochondrial inner membrane; involved in turnover of membrane-embedded proteins; mediates degradation of Cox1p in coa2 mutant cells; member of a family of predicted membrane-bound metallopeptidases in prokaryotes and higher eukaryotes Gene:PEP7(YDR323C)|FD-Score:6.03|P-value:8.13E-10||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PEX11(YOL147C)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Peroxisomal membrane protein required for medium-chain fatty acid oxidation and peroxisome proliferation, possibly by inducing membrane curvature; localization regulated by phosphorylation; transcription regulated by Adr1p and Pip2p-Oaf1p Gene:PHO84(YML123C)|FD-Score:6.45|P-value:5.50E-11||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PHO85(YPL031C)|FD-Score:-3.13|P-value:8.62E-4||SGD DESC:Cyclin-dependent kinase, with ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle Gene:PIH1(YHR034C)|FD-Score:3.97|P-value:3.57E-5||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:PMT5(YDL093W)|FD-Score:-3.11|P-value:9.37E-4||SGD DESC:Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt3p, can instead interact with Pmt2p in some conditions; target for new antifungals Gene:POL32(YJR043C)|FD-Score:4.13|P-value:1.81E-5||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:PPM1(YDR435C)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:PPT1(YGR123C)|FD-Score:4.14|P-value:1.70E-5||SGD DESC:Protein serine/threonine phosphatase, regulates Hsp90 chaperone by affecting its ATPase and cochaperone binding activities; has similarity to human phosphatase PP5; present in both the nucleus and cytoplasm; expressed during logarithmic growth Gene:PRM1(YNL279W)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Pheromone-regulated multispanning membrane protein involved in membrane fusion during mating; predicted to have 5 transmembrane segments and a coiled coil domain; localizes to the shmoo tip; regulated by Ste12p Gene:PYK2(YOR347C)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication Gene:RAD16(YBR114W)|FD-Score:-3.22|P-value:6.36E-4||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RAD33(YML011C)|FD-Score:4.56|P-value:2.53E-6||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:RGD1(YBR260C)|FD-Score:-3.33|P-value:4.34E-4||SGD DESC:GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization Gene:RIM1(YCR028C-A)|FD-Score:3.13|P-value:8.60E-4||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RIM8(YGL045W)|FD-Score:3.11|P-value:9.22E-4||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RPA12(YJR063W)|FD-Score:6.79|P-value:5.42E-12||SGD DESC:RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex Gene:RPL22B(YFL034C-A)|FD-Score:-3.1|P-value:9.66E-4||SGD DESC:Ribosomal 60S subunit protein L22B; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication Gene:RRD2(YPL152W)|FD-Score:4.01|P-value:2.97E-5||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; also activates the phosphotyrosyl phosphatase activity of protein phosphatase 2A (PP2A); regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex; protein abundance increases in response to DNA replication stress Gene:RRG1(YDR065W)|FD-Score:3.73|P-value:9.70E-5||SGD DESC:Protein of unknown function, required for vacuolar acidification and mitochondrial genome maintenance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SER2(YGR208W)|FD-Score:4.64|P-value:1.70E-6||SGD DESC:Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source Gene:SHE4(YOR035C)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SIC1(YLR079W)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 Gene:SIL1(YOL031C)|FD-Score:5.1|P-value:1.74E-7||SGD DESC:Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein Gene:SIN4(YNL236W)|FD-Score:5.19|P-value:1.05E-7||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SMY1(YKL079W)|FD-Score:5.09|P-value:1.76E-7||SGD DESC:Protein that interacts with Myo2p, proposed to be involved in exocytosis; N-terminal domain is related to the motor domain of kinesins Gene:SOD1(YJR104C)|FD-Score:4.16|P-value:1.60E-5||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SSN8(YNL025C)|FD-Score:3.84|P-value:6.07E-5||SGD DESC:Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance Gene:SWI6(YLR182W)|FD-Score:3.15|P-value:8.03E-4||SGD DESC:Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators Gene:TAT1(YBR069C)|FD-Score:5.28|P-value:6.56E-8||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:THR1(YHR025W)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TIP41(YPR040W)|FD-Score:-3.18|P-value:7.28E-4||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:TRK2(YKR050W)|FD-Score:-3.31|P-value:4.74E-4||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; TRK2 has a paralog, TRK1, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:6|P-value:9.70E-10||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:7.8|P-value:3.13E-15||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:6.15|P-value:3.77E-10||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TUB3(YML124C)|FD-Score:8.66|P-value:2.45E-18||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UME6(YDR207C)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p Gene:UPS2(YLR168C)|FD-Score:3.87|P-value:5.39E-5||SGD DESC:Mitochondrial protein involved in phospholipid metabolism; localizes to the intermembrane space; has a role in regulation of phospholipid metabolism by inhibiting conversion of phosphatidylethanolamine to phosphatidylcholine; null mutant has defects in mitochondrial morphology; similar to Ups1p, Ups3p and to human PRELI Gene:VID27(YNL212W)|FD-Score:-3.42|P-value:3.10E-4||SGD DESC:Cytoplasmic protein of unknown function; possibly involved in vacuolar protein degradation; not essential for proteasome-dependent degradation of fructose-1,6-bisphosphatase (FBPase); null mutants exhibit normal growth Gene:VNX1(YNL321W)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate Gene:YAP1(YML007W)|FD-Score:9.3|P-value:7.31E-21||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YBL062W(YBL062W_d)|FD-Score:3.81|P-value:7.08E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR224W(YBR224W_d)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TDP1 Gene:YCR043C(YCR043C_p)|FD-Score:3.93|P-value:4.20E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene Gene:YDL094C(YDL094C_d)|FD-Score:-4.15|P-value:1.66E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verfied gene PMT5/YDL093W; YDL094C is not essential Gene:YDR008C(YDR008C_d)|FD-Score:7.25|P-value:2.05E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR093W(YGR093W_p)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:Putative protein of unconfirmed function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:YGR121W-A(YGR121W-A_p)|FD-Score:4.08|P-value:2.29E-5||SGD DESC:Putative protein of unknown function Gene:YIL168W(YIL168W)|FD-Score:-3.49|P-value:2.44E-4||SGD DESC:Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other <i>S. cerevisiae</i> strain backgrounds YIL168W and adjacent ORF, YIL167W, constitute a single ORF encoding L-serine dehydratase Gene:YIR042C(YIR042C_p)|FD-Score:-3.39|P-value:3.50E-4||SGD DESC:Putative protein of unknown function; YIR042C is a non-essential gene Gene:YJL120W(YJL120W_d)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YJR018W(YJR018W_d)|FD-Score:3.79|P-value:7.59E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR047W(YKR047W_d)|FD-Score:5.53|P-value:1.59E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1 Gene:YLR202C(YLR202C_d)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron Gene:YML037C(YML037C_p)|FD-Score:-3.39|P-value:3.51E-4||SGD DESC:Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene Gene:YMR119W-A(YMR119W-A_d)|FD-Score:-3.2|P-value:6.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR206W(YMR206W_p)|FD-Score:4.16|P-value:1.58E-5||SGD DESC:Putative protein of unknown function; not an essential gene; YMR206W has a paralog, YNR014W, that arose from the whole genome duplication Gene:YNL143C(YNL143C_p)|FD-Score:-5.33|P-value:4.88E-8||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YNL184C(YNL184C_p)|FD-Score:4.4|P-value:5.39E-6||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOR376W-A(YOR376W-A_p)|FD-Score:4.11|P-value:2.01E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPK1(YKL126W)|FD-Score:4.38|P-value:5.99E-6||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL035C(YPL035C_d)|FD-Score:4.38|P-value:5.81E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YPL034W; YPL035C is not an essential gene Gene:YPR146C(YPR146C_d)|FD-Score:3.93|P-value:4.30E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ABZ1(YNR033W)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Para-aminobenzoate (PABA) synthase; has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis; protein abundance increases in response to DNA replication stress Gene:AIM43(YPL099C)|FD-Score:5.5|P-value:1.88E-8||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ALD6(YPL061W)|FD-Score:4.7|P-value:1.29E-6||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:ALF1(YNL148C)|FD-Score:7.91|P-value:1.32E-15||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ARO1(YDR127W)|FD-Score:4.46|P-value:4.16E-6||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:BLM10(YFL007W)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Proteasome activator; binds the core proteasome and stimulates proteasome-mediated protein degradation by inducing gate opening; required for resistance to bleomycin, may be involved in protecting against oxidative damage; similar to mammalian PA200 Gene:BRO1(YPL084W)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes Gene:BUD19(YJL188C_d)|FD-Score:-3.62|P-value:1.50E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:BUD27(YFL023W)|FD-Score:5.03|P-value:2.44E-7||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:CHO1(YER026C)|FD-Score:4.01|P-value:3.10E-5||SGD DESC:Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline Gene:CHS7(YHR142W)|FD-Score:3.18|P-value:7.41E-4||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:CIN1(YOR349W)|FD-Score:3.22|P-value:6.41E-4||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:COX6(YHR051W)|FD-Score:4.73|P-value:1.13E-6||SGD DESC:Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels Gene:CPR5(YDR304C)|FD-Score:-3.66|P-value:1.25E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER Gene:DAL81(YIR023W)|FD-Score:8.76|P-value:1.02E-18||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DBF2(YGR092W)|FD-Score:3.98|P-value:3.39E-5||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DSE2(YHR143W)|FD-Score:7.35|P-value:1.00E-13||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:DUG2(YBR281C)|FD-Score:3.85|P-value:6.02E-5||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:ECM38(YLR299W)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Gamma-glutamyltranspeptidase, major glutathione-degrading enzyme; involved in detoxification of electrophilic xenobiotics; expression induced mainly by nitrogen starvation Gene:EKI1(YDR147W)|FD-Score:3.94|P-value:4.00E-5||SGD DESC:Ethanolamine kinase; primarily responsible for phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; exhibits some choline kinase activity, thus contributing to phosphatidylcholine synthesis via the CDP-choline pathway; EKI1 has a paralog, CKI1, that arose from the whole genome duplication Gene:ELF1(YKL160W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Transcription elongation factor that contains a conserved zinc finger domain; implicated in the maintenance of proper chromatin structure in actively transcribed regions; deletion inhibits Brome mosaic virus (BMV) gene expression Gene:EXO5(YBR163W)|FD-Score:4.94|P-value:3.83E-7||SGD DESC:Mitochondrial 5'-3' exonuclease and sliding exonuclease, required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2 Gene:GBP2(YCL011C)|FD-Score:-3.17|P-value:7.65E-4||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; also binds single-stranded telomeric repeat sequence in vitro; GBP2 has a paralog, HRB1, that arose from the whole genome duplication Gene:GCV1(YDR019C)|FD-Score:-3.31|P-value:4.66E-4||SGD DESC:T subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm Gene:GLE2(YER107C)|FD-Score:11.6|P-value:1.20E-31||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GOS1(YHL031C)|FD-Score:7.61|P-value:1.32E-14||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:HFI1(YPL254W)|FD-Score:3.99|P-value:3.28E-5||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:HHY1(YEL059W_d)|FD-Score:-3.95|P-value:3.87E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; mutant is hypersensitive to hygromycin B indicative of defects in vacuolar trafficking Gene:HMT1(YBR034C)|FD-Score:-4.02|P-value:2.97E-5||SGD DESC:Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region. Gene:HOM6(YJR139C)|FD-Score:5.05|P-value:2.16E-7||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HRT3(YLR097C)|FD-Score:3.25|P-value:5.82E-4||SGD DESC:Putative SCF-ubiquitin ligase F-box protein, based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies Gene:HTZ1(YOL012C)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin Gene:HXK2(YGL253W)|FD-Score:-6.75|P-value:7.20E-12||SGD DESC:Hexokinase isoenzyme 2; catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene; phosphorylation/dephosphorylation at serine-14 by protein kinase Snf1p and protein phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; HXK2 has a paralog, HXK1, that arose from the whole genome duplication Gene:HYR1(YIR037W)|FD-Score:4.23|P-value:1.18E-5||SGD DESC:Thiol peroxidase that functions as a hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce a redox signal to the Yap1p transcription factor Gene:ICS2(YBR157C)|FD-Score:3.2|P-value:6.83E-4||SGD DESC:Protein of unknown function; null mutation does not confer any obvious defects in growth, spore germination, viability, or carbohydrate utilization Gene:IFM1(YOL023W)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Mitochondrial translation initiation factor 2 Gene:ILV1(YER086W)|FD-Score:4.85|P-value:6.03E-7||SGD DESC:Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation Gene:IMG2(YCR071C)|FD-Score:3.72|P-value:9.99E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:INP1(YMR204C)|FD-Score:-3.94|P-value:4.15E-5||SGD DESC:Peripheral membrane protein of peroxisomes involved in peroxisomal inheritance; recruitment to peroxisomes is mediated by interaction with Pex3p at the peroxisomal membrane Gene:IRC11(YOR013W_d)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Dubious opening reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YOR012C; null mutant displays increased levels of spontaneous Rad52 foci Gene:IRC21(YMR073C)|FD-Score:4.18|P-value:1.46E-5||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:KEL3(YPL263C)|FD-Score:-3.16|P-value:7.99E-4||SGD DESC:Cytoplasmic protein of unknown function Gene:LAP3(YNL239W)|FD-Score:6.38|P-value:9.07E-11||SGD DESC:Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH Gene:LCB4(YOR171C)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes Gene:MF(ALPHA)1(YPL187W)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Mating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)2, although MF(ALPHA)1 produces most alpha-factor Gene:MOH1(YBL049W)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:MPC54(YOR177C)|FD-Score:4.67|P-value:1.47E-6||SGD DESC:Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate Gene:MRP51(YPL118W)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MRPL4(YLR439W)|FD-Score:3.24|P-value:6.03E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:MSB2(YGR014W)|FD-Score:-4.72|P-value:1.16E-6||SGD DESC:Mucin family member involved in signaling; functions in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; is processed into secreted and cell-associated forms by the aspartyl protease, Yps1p; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate Gene:MSI1(YBR195C)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription; Msi1p localizes to both nucleus and cytoplasm and has an independent role as a negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase Gene:MSM1(YGR171C)|FD-Score:11|P-value:2.69E-28||SGD DESC:Mitochondrial methionyl-tRNA synthetase (MetRS), functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p Gene:MTG1(YMR097C)|FD-Score:5.98|P-value:1.11E-9||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:MUD2(YKL074C)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65 Gene:NCS6(YGL211W)|FD-Score:4.62|P-value:1.92E-6||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:NIP100(YPL174C)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) Gene:NMA2(YGR010W)|FD-Score:-4.18|P-value:1.47E-5||SGD DESC:Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in de novo and salvage synthesis of NAD(+); NMA2 has a paralog, NMA1, that arose from the whole genome duplication Gene:NUP2(YLR335W)|FD-Score:3.89|P-value:4.92E-5||SGD DESC:Nucleoporin involved in nucleocytoplasmic transport, binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization Gene:OMA1(YKR087C)|FD-Score:5.41|P-value:3.08E-8||SGD DESC:Metalloendopeptidase of the mitochondrial inner membrane; involved in turnover of membrane-embedded proteins; mediates degradation of Cox1p in coa2 mutant cells; member of a family of predicted membrane-bound metallopeptidases in prokaryotes and higher eukaryotes Gene:PEP7(YDR323C)|FD-Score:6.03|P-value:8.13E-10||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PEX11(YOL147C)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Peroxisomal membrane protein required for medium-chain fatty acid oxidation and peroxisome proliferation, possibly by inducing membrane curvature; localization regulated by phosphorylation; transcription regulated by Adr1p and Pip2p-Oaf1p Gene:PHO84(YML123C)|FD-Score:6.45|P-value:5.50E-11||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PHO85(YPL031C)|FD-Score:-3.13|P-value:8.62E-4||SGD DESC:Cyclin-dependent kinase, with ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle Gene:PIH1(YHR034C)|FD-Score:3.97|P-value:3.57E-5||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:PMT5(YDL093W)|FD-Score:-3.11|P-value:9.37E-4||SGD DESC:Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt3p, can instead interact with Pmt2p in some conditions; target for new antifungals Gene:POL32(YJR043C)|FD-Score:4.13|P-value:1.81E-5||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:PPM1(YDR435C)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:PPT1(YGR123C)|FD-Score:4.14|P-value:1.70E-5||SGD DESC:Protein serine/threonine phosphatase, regulates Hsp90 chaperone by affecting its ATPase and cochaperone binding activities; has similarity to human phosphatase PP5; present in both the nucleus and cytoplasm; expressed during logarithmic growth Gene:PRM1(YNL279W)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Pheromone-regulated multispanning membrane protein involved in membrane fusion during mating; predicted to have 5 transmembrane segments and a coiled coil domain; localizes to the shmoo tip; regulated by Ste12p Gene:PYK2(YOR347C)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication Gene:RAD16(YBR114W)|FD-Score:-3.22|P-value:6.36E-4||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RAD33(YML011C)|FD-Score:4.56|P-value:2.53E-6||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:RGD1(YBR260C)|FD-Score:-3.33|P-value:4.34E-4||SGD DESC:GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization Gene:RIM1(YCR028C-A)|FD-Score:3.13|P-value:8.60E-4||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RIM8(YGL045W)|FD-Score:3.11|P-value:9.22E-4||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RPA12(YJR063W)|FD-Score:6.79|P-value:5.42E-12||SGD DESC:RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex Gene:RPL22B(YFL034C-A)|FD-Score:-3.1|P-value:9.66E-4||SGD DESC:Ribosomal 60S subunit protein L22B; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication Gene:RRD2(YPL152W)|FD-Score:4.01|P-value:2.97E-5||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; also activates the phosphotyrosyl phosphatase activity of protein phosphatase 2A (PP2A); regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex; protein abundance increases in response to DNA replication stress Gene:RRG1(YDR065W)|FD-Score:3.73|P-value:9.70E-5||SGD DESC:Protein of unknown function, required for vacuolar acidification and mitochondrial genome maintenance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SER2(YGR208W)|FD-Score:4.64|P-value:1.70E-6||SGD DESC:Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source Gene:SHE4(YOR035C)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SIC1(YLR079W)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 Gene:SIL1(YOL031C)|FD-Score:5.1|P-value:1.74E-7||SGD DESC:Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein Gene:SIN4(YNL236W)|FD-Score:5.19|P-value:1.05E-7||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SMY1(YKL079W)|FD-Score:5.09|P-value:1.76E-7||SGD DESC:Protein that interacts with Myo2p, proposed to be involved in exocytosis; N-terminal domain is related to the motor domain of kinesins Gene:SOD1(YJR104C)|FD-Score:4.16|P-value:1.60E-5||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SSN8(YNL025C)|FD-Score:3.84|P-value:6.07E-5||SGD DESC:Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance Gene:SWI6(YLR182W)|FD-Score:3.15|P-value:8.03E-4||SGD DESC:Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators Gene:TAT1(YBR069C)|FD-Score:5.28|P-value:6.56E-8||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:THR1(YHR025W)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TIP41(YPR040W)|FD-Score:-3.18|P-value:7.28E-4||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:TRK2(YKR050W)|FD-Score:-3.31|P-value:4.74E-4||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; TRK2 has a paralog, TRK1, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:6|P-value:9.70E-10||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:7.8|P-value:3.13E-15||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:6.15|P-value:3.77E-10||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TUB3(YML124C)|FD-Score:8.66|P-value:2.45E-18||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UME6(YDR207C)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p Gene:UPS2(YLR168C)|FD-Score:3.87|P-value:5.39E-5||SGD DESC:Mitochondrial protein involved in phospholipid metabolism; localizes to the intermembrane space; has a role in regulation of phospholipid metabolism by inhibiting conversion of phosphatidylethanolamine to phosphatidylcholine; null mutant has defects in mitochondrial morphology; similar to Ups1p, Ups3p and to human PRELI Gene:VID27(YNL212W)|FD-Score:-3.42|P-value:3.10E-4||SGD DESC:Cytoplasmic protein of unknown function; possibly involved in vacuolar protein degradation; not essential for proteasome-dependent degradation of fructose-1,6-bisphosphatase (FBPase); null mutants exhibit normal growth Gene:VNX1(YNL321W)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate Gene:YAP1(YML007W)|FD-Score:9.3|P-value:7.31E-21||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YBL062W(YBL062W_d)|FD-Score:3.81|P-value:7.08E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR224W(YBR224W_d)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TDP1 Gene:YCR043C(YCR043C_p)|FD-Score:3.93|P-value:4.20E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene Gene:YDL094C(YDL094C_d)|FD-Score:-4.15|P-value:1.66E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verfied gene PMT5/YDL093W; YDL094C is not essential Gene:YDR008C(YDR008C_d)|FD-Score:7.25|P-value:2.05E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR093W(YGR093W_p)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:Putative protein of unconfirmed function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:YGR121W-A(YGR121W-A_p)|FD-Score:4.08|P-value:2.29E-5||SGD DESC:Putative protein of unknown function Gene:YIL168W(YIL168W)|FD-Score:-3.49|P-value:2.44E-4||SGD DESC:Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other <i>S. cerevisiae</i> strain backgrounds YIL168W and adjacent ORF, YIL167W, constitute a single ORF encoding L-serine dehydratase Gene:YIR042C(YIR042C_p)|FD-Score:-3.39|P-value:3.50E-4||SGD DESC:Putative protein of unknown function; YIR042C is a non-essential gene Gene:YJL120W(YJL120W_d)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YJR018W(YJR018W_d)|FD-Score:3.79|P-value:7.59E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR047W(YKR047W_d)|FD-Score:5.53|P-value:1.59E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1 Gene:YLR202C(YLR202C_d)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron Gene:YML037C(YML037C_p)|FD-Score:-3.39|P-value:3.51E-4||SGD DESC:Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene Gene:YMR119W-A(YMR119W-A_d)|FD-Score:-3.2|P-value:6.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR206W(YMR206W_p)|FD-Score:4.16|P-value:1.58E-5||SGD DESC:Putative protein of unknown function; not an essential gene; YMR206W has a paralog, YNR014W, that arose from the whole genome duplication Gene:YNL143C(YNL143C_p)|FD-Score:-5.33|P-value:4.88E-8||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YNL184C(YNL184C_p)|FD-Score:4.4|P-value:5.39E-6||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOR376W-A(YOR376W-A_p)|FD-Score:4.11|P-value:2.01E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPK1(YKL126W)|FD-Score:4.38|P-value:5.99E-6||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL035C(YPL035C_d)|FD-Score:4.38|P-value:5.81E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YPL034W; YPL035C is not an essential gene Gene:YPR146C(YPR146C_d)|FD-Score:3.93|P-value:4.30E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR527W30.102.27E-19914.60RBA50Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1
YOR207C15.501.06E-547.20RET1Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
YOR262W8.333.88E-171.48GPN2Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion
YHR062C6.853.62E-121.52RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YKL195W5.334.87E-80.12MIA40Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p
YOR204W5.219.62E-80.09DED1ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility
YKL111C_d5.121.57E-70.19YKL111C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene ABF1
YMR185W_p4.924.23E-70.10RTP1_pProtein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene
YNR016C4.836.88E-70.06ACC1Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
YLR243W_p4.779.27E-70.11GPN3_pPutative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p
YDL193W4.661.57E-60.17NUS1Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY
YDL209C4.493.60E-60.26CWC2Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2
YJL039C4.221.20E-50.10NUP192Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205
YDL150W4.121.86E-50.12RPC53RNA polymerase III subunit C53
YFR004W4.003.11E-50.09RPN11Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YER107C11.601.20E-31GLE2RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1
YGR171C11.002.69E-28MSM1Mitochondrial methionyl-tRNA synthetase (MetRS), functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p
YML007W9.307.31E-21YAP1Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YIR023W8.761.02E-18DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YML124C8.662.45E-18TUB3Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication
YNL148C7.911.32E-15ALF1Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance
YKL211C7.803.13E-15TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YHL031C7.611.32E-14GOS1v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28
YHR143W7.351.00E-13DSE2Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP
YDR008C_d7.252.05E-13YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YJR063W6.795.42E-12RPA12RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex
YML123C6.455.50E-11PHO84High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity
YNL239W6.389.07E-11LAP3Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH
YDR354W6.153.77E-10TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YDR323C6.038.13E-10PEP7Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance

GO enrichment analysis for SGTC_2921
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3362.64E-155SGTC_20044023728 41.9 μMChembridge (Fragment library)3600840.0877193
0.3061.36E-128SGTC_1069diphenylcyclopropenone 8.5 μMNIH Clinical Collection650570.08
0.2801.08E-106SGTC_870100-0052 35.5 μMChemDiv (Drug-like library)2001870.27451
0.2429.67E-80SGTC_6113453-1652 23.7 μMChemDiv (Drug-like library)15605750.136986
0.2391.93E-77SGTC_6390833-0042 114.0 μMChemDiv (Drug-like library)59789110.136364
0.2371.82E-75SGTC_2103551-0445 56.8 μMChemDiv (Drug-like library)28651800.173333
0.2328.01E-73SGTC_2233sulbentine 40.4 μMMiscellaneous676860.112903
0.2308.95E-72SGTC_8201582-0060 59.9 μMChemDiv (Drug-like library)38488400.0857143
0.2283.58E-70SGTC_24305571206 200.0 μMMiscellaneous17317710.0945946
0.2126.31E-61SGTC_22467293507 91.6 μMChembridge (Fragment library)8876560.126984
0.2113.02E-60SGTC_15852',4'-dihydroxychalcone 10.4 μMTimTec (Pure natural product library)53572180.0967742
0.2104.50E-60SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.164384
0.2034.90E-56SGTC_21515563267 85.4 μMChembridge (Fragment library)53108240.0615385
0.2035.57E-56SGTC_5891227-0070 34.1 μMChemDiv (Drug-like library)28413840.104478
0.2014.86E-55SGTC_8600443-0016 25.1 μMChemDiv (Drug-like library)53379420.09375

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_253dyclonine31.25 μM0.4444443180Miscellaneous289.412484.05303amide catabolism
SGTC_14234048-01125.59 μM0.4375X1423ChemDiv (Drug-like library)425.004475.7840
SGTC_2249haloperidol116.36 μM0.406783559Miscellaneous375.8642233.88714ergosterol depletion effects on membrane
SGTC_258haloperidol50.78 μM0.406783559Miscellaneous375.8642233.88714fatty acid desaturase (OLE1)
SGTC_2725haloperidol53.21 μM0.406783559Miscellaneous375.8642233.88714plasma membrane duress
SGTC_1957st07703562.4 μM0.4705593TimTec (Natural product derivative library)278.73413.19212
SGTC_11594092-053861.2 μM0.388889717301ChemDiv (Drug-like library)332.7997233.65403
SGTC_1068loratadine26.9 μM0.3787883957Miscellaneous382.88322503
SGTC_3027908954149.47 μM0.370379943819Chembridge (Drug-like library)288.427723.92802fatty acid desaturase (OLE1)
SGTC_14823346-20498.48 μM0.3692313826804ChemDiv (Drug-like library)390.516363.84115
SGTC_3033909088749.47 μM0.35714319325384Chembridge (Drug-like library)342.862423.9402fatty acid desaturase (OLE1)
SGTC_5681193-00977.48 μM0.3461543646150ChemDiv (Drug-like library)242.658941.98724ERAD & cell cycle