7999257

2-benzylsulfanyl-N-(3,5-difluorophenyl)acetamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2922
Screen concentration 32.5 μM
Source Chembridge (Drug-like library)
PubChem CID 2988377
SMILES C1=CC=C(C=C1)CSCC(=O)NC2=CC(=CC(=C2)F)F
Standardized SMILES Fc1cc(F)cc(NC(=O)CSCc2ccccc2)c1
Molecular weight 293.3316
ALogP 3.43
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.45
% growth inhibition (Hom. pool) 1.16


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2988377
Download HIP data (tab-delimited text)  (excel)
Gene:APC11(YDL008W)|FD-Score:5.55|P-value:1.44E-8|Clearance:0.69||SGD DESC:Catalytic core subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; contains a RING-H2 domain that is required for activity Gene:CDC21(YOR074C)|FD-Score:4.63|P-value:1.85E-6|Clearance:0.69||SGD DESC:Thymidylate synthase, required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S Gene:CDC3(YLR314C)|FD-Score:3.34|P-value:4.20E-4|Clearance:0.03||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CWC25(YNL245C)|FD-Score:-4.45|P-value:4.33E-6|Clearance:0||SGD DESC:Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p Gene:DED81(YHR019C)|FD-Score:5.09|P-value:1.83E-7|Clearance:0.69||SGD DESC:Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA Gene:FMN1(YDR236C)|FD-Score:-7.72|P-value:5.83E-15|Clearance:0||SGD DESC:Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane Gene:GPM1(YKL152C)|FD-Score:-3.11|P-value:9.50E-4|Clearance:0||SGD DESC:Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis Gene:KEG1(YFR042W)|FD-Score:3.51|P-value:2.21E-4|Clearance:0.03||SGD DESC:Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability Gene:MPS2(YGL075C)|FD-Score:-3.76|P-value:8.49E-5|Clearance:0||SGD DESC:Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p Gene:NIC96(YFR002W)|FD-Score:4.99|P-value:3.08E-7|Clearance:0.69||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:PSF1(YDR013W)|FD-Score:3.31|P-value:4.71E-4|Clearance:0.23||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RAP1(YNL216W)|FD-Score:-3.21|P-value:6.71E-4|Clearance:0||SGD DESC:Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain) Gene:RPN9(YDR427W)|FD-Score:-4.36|P-value:6.45E-6|Clearance:0||SGD DESC:Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress Gene:RPT1(YKL145W)|FD-Score:3.53|P-value:2.09E-4|Clearance:0.01||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:RRN6(YBL014C)|FD-Score:3.48|P-value:2.48E-4|Clearance:0.14||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:SPB1(YCL054W)|FD-Score:5.23|P-value:8.65E-8|Clearance:0.69||SGD DESC:AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants Gene:TEN1(YLR010C)|FD-Score:3.94|P-value:4.05E-5|Clearance:0.32||SGD DESC:Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p Gene:TRS31(YDR472W)|FD-Score:3.62|P-value:1.49E-4|Clearance:0.09||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:APC11(YDL008W)|FD-Score:5.55|P-value:1.44E-8|Clearance:0.69||SGD DESC:Catalytic core subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; contains a RING-H2 domain that is required for activity Gene:CDC21(YOR074C)|FD-Score:4.63|P-value:1.85E-6|Clearance:0.69||SGD DESC:Thymidylate synthase, required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S Gene:CDC3(YLR314C)|FD-Score:3.34|P-value:4.20E-4|Clearance:0.03||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CWC25(YNL245C)|FD-Score:-4.45|P-value:4.33E-6|Clearance:0||SGD DESC:Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p Gene:DED81(YHR019C)|FD-Score:5.09|P-value:1.83E-7|Clearance:0.69||SGD DESC:Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA Gene:FMN1(YDR236C)|FD-Score:-7.72|P-value:5.83E-15|Clearance:0||SGD DESC:Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane Gene:GPM1(YKL152C)|FD-Score:-3.11|P-value:9.50E-4|Clearance:0||SGD DESC:Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis Gene:KEG1(YFR042W)|FD-Score:3.51|P-value:2.21E-4|Clearance:0.03||SGD DESC:Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability Gene:MPS2(YGL075C)|FD-Score:-3.76|P-value:8.49E-5|Clearance:0||SGD DESC:Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p Gene:NIC96(YFR002W)|FD-Score:4.99|P-value:3.08E-7|Clearance:0.69||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:PSF1(YDR013W)|FD-Score:3.31|P-value:4.71E-4|Clearance:0.23||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RAP1(YNL216W)|FD-Score:-3.21|P-value:6.71E-4|Clearance:0||SGD DESC:Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain) Gene:RPN9(YDR427W)|FD-Score:-4.36|P-value:6.45E-6|Clearance:0||SGD DESC:Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress Gene:RPT1(YKL145W)|FD-Score:3.53|P-value:2.09E-4|Clearance:0.01||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:RRN6(YBL014C)|FD-Score:3.48|P-value:2.48E-4|Clearance:0.14||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:SPB1(YCL054W)|FD-Score:5.23|P-value:8.65E-8|Clearance:0.69||SGD DESC:AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants Gene:TEN1(YLR010C)|FD-Score:3.94|P-value:4.05E-5|Clearance:0.32||SGD DESC:Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p Gene:TRS31(YDR472W)|FD-Score:3.62|P-value:1.49E-4|Clearance:0.09||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2988377
Download HOP data (tab-delimited text)  (excel)
Gene:ADK2(YER170W)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Mitochondrial adenylate kinase, catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background Gene:ALG3(YBL082C)|FD-Score:-4.48|P-value:3.73E-6||SGD DESC:Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins Gene:ANP1(YEL036C)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:APJ1(YNL077W)|FD-Score:5.77|P-value:4.04E-9||SGD DESC:Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:ATG15(YCR068W)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway Gene:ATP11(YNL315C)|FD-Score:-3.79|P-value:7.40E-5||SGD DESC:Molecular chaperone, required for the assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase Gene:BIO2(YGR286C)|FD-Score:-4.02|P-value:2.86E-5||SGD DESC:Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant Gene:COX19(YLL018C-A)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:CRC1(YOR100C)|FD-Score:-3.25|P-value:5.72E-4||SGD DESC:Mitochondrial inner membrane carnitine transporter, required for carnitine-dependent transport of acetyl-CoA from peroxisomes to mitochondria during fatty acid beta-oxidation Gene:CRR1(YLR213C)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Putative glycoside hydrolase of the spore wall envelope; required for normal spore wall assembly, possibly for cross-linking between the glucan and chitosan layers; expressed during sporulation Gene:CUP2(YGL166W)|FD-Score:-3.53|P-value:2.11E-4||SGD DESC:Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; CUP2 has a paralog, HAA1, that arose from the whole genome duplication Gene:DFG5(YMR238W)|FD-Score:6.15|P-value:3.87E-10||SGD DESC:Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p Gene:DUR3(YHL016C)|FD-Score:-3.41|P-value:3.19E-4||SGD DESC:Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway Gene:DUS4(YLR405W)|FD-Score:3.16|P-value:7.91E-4||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p Gene:EEB1(YPL095C)|FD-Score:-4.51|P-value:3.25E-6||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification; EEB1 has a paralog, EHT1, that arose from the whole genome duplication Gene:ENT5(YDR153C)|FD-Score:3.18|P-value:7.40E-4||SGD DESC:Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p, clathrin adaptor complex AP-1, and clathrin Gene:EXG1(YLR300W)|FD-Score:-3.81|P-value:6.88E-5||SGD DESC:Major exo-1,3-beta-glucanase of the cell wall, involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes Gene:GAT2(YMR136W)|FD-Score:-5.57|P-value:1.30E-8||SGD DESC:Protein containing GATA family zinc finger motifs; similar to Gln3p and Dal80p; expression repressed by leucine Gene:GIS2(YNL255C)|FD-Score:-3.83|P-value:6.45E-5||SGD DESC:Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 Gene:INP1(YMR204C)|FD-Score:-3.99|P-value:3.33E-5||SGD DESC:Peripheral membrane protein of peroxisomes involved in peroxisomal inheritance; recruitment to peroxisomes is mediated by interaction with Pex3p at the peroxisomal membrane Gene:JSN1(YJR091C)|FD-Score:3.21|P-value:6.61E-4||SGD DESC:Member of the Puf family of RNA-binding proteins, interacts with mRNAs encoding membrane-associated proteins; involved in localizing the Arp2/3 complex to mitochondria; overexpression causes increased sensitivity to benomyl Gene:MFM1(YPL060W)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p Gene:MRPL20(YKR085C)|FD-Score:3.17|P-value:7.58E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSS18(YPR134W)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions Gene:MTM1(YGR257C)|FD-Score:5.1|P-value:1.71E-7||SGD DESC:Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor Gene:OSW5(YMR148W)|FD-Score:-3.32|P-value:4.56E-4||SGD DESC:Protein of unknown function that may play a role in spore wall assembly; predicted to contain an N-terminal transmembrane domain; osw5 null mutant spores exhibit increased spore wall permeability and sensitivity to beta-glucanase digestion Gene:PBS2(YJL128C)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PUT4(YOR348C)|FD-Score:-3.51|P-value:2.21E-4||SGD DESC:Proline permease, required for high-affinity transport of proline; also transports the toxic proline analog azetidine-2-carboxylate (AzC); PUT4 transcription is repressed in ammonia-grown cells Gene:RAD5(YLR032W)|FD-Score:4.82|P-value:7.14E-7||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:REX4(YOL080C)|FD-Score:-3.37|P-value:3.72E-4||SGD DESC:Putative RNA exonuclease possibly involved in pre-rRNA processing and ribosome assembly Gene:RNH202(YDR279W)|FD-Score:4.55|P-value:2.68E-6||SGD DESC:Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS2 that causes Aicardi-Goutieres syndrome Gene:RPL19A(YBR084C-A)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPS12(YOR369C)|FD-Score:-3.26|P-value:5.66E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S12, no bacterial homolog Gene:RPS29A(YLR388W)|FD-Score:4.5|P-value:3.38E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication Gene:RRT14(YIL127C_p)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Putative protein of unknown function; identified in a screen for mutants with decreased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis Gene:RRT16(YNL105W_d)|FD-Score:-3.58|P-value:1.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene INP52; identified in a screen for mutants with decreased levels of rDNA transcription Gene:RSM28(YDR494W)|FD-Score:-3.52|P-value:2.18E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation Gene:RTC6(YPL183W-A)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:RTN1(YDR233C)|FD-Score:-3.1|P-value:9.67E-4||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; mutant overexpressing RTN1 shows increase in tubular ER; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; more abundant than Rtn2p; member of the RTNLA subfamily; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; RTN1 has a paralog, RTN2, that arose from the whole genome duplication Gene:RUD3(YOR216C)|FD-Score:-5.18|P-value:1.13E-7||SGD DESC:Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1 Gene:SAE3(YHR079C-A)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Mei5p; proposed to be an assembly factor for Dmc1p Gene:SFL1(YOR140W)|FD-Score:-3.38|P-value:3.60E-4||SGD DESC:Transcriptional repressor and activator; involved in repression of flocculation-related genes, and activation of stress responsive genes; negatively regulated by cAMP-dependent protein kinase A subunit Tpk2p Gene:SPO71(YDR104C)|FD-Score:-3.38|P-value:3.62E-4||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation, in particular for the proper size of the prospore membrane (PSM); interacts with SPO1; dispensable for both nuclear divisions during meiosis; contains two PH domains Gene:SPO77(YLR341W)|FD-Score:3.32|P-value:4.44E-4||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis Gene:SSB1(YDL229W)|FD-Score:-3.87|P-value:5.46E-5||SGD DESC:Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in folding of newly-made polypeptide chains; member of the HSP70 family; interacts with phosphatase subunit Reg1p; SSB1 has a paralog, SSB2, that arose from the whole genome duplication Gene:TEC1(YBR083W)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Transcription factor targeting filamentation genes and Ty1 expression; Ste12p activation of most filamentation gene promoters depends on Tec1p and Tec1p transcriptional activity is dependent on its association with Ste12p; binds to TCS elements upstream of filamentation genes, which are regulated by Tec1p/Ste12p/Dig1p complex; competes with Dig2p for binding to Ste12p/Dig1p; positive regulator of chronological life span; TEA/ATTS DNA-binding domain family member Gene:WAR1(YML076C)|FD-Score:-6.88|P-value:3.09E-12||SGD DESC:Homodimeric Zn2Cys6 zinc finger transcription factor; binds to a weak acid response element to induce transcription of PDR12 and FUN34, encoding an acid transporter and a putative ammonia transporter, respectively Gene:YBR232C(YBR232C_d)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBT1(YLL048C)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters Gene:YER087C-A(YER087C-A_d)|FD-Score:-3.51|P-value:2.28E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; overlaps the uncharacterized gene YER087W, a putative tRNA synthetase Gene:YGR001C(YGR001C_p)|FD-Score:-3.59|P-value:1.67E-4||SGD DESC:Putative protein of unknown function with similarity to methyltransferase family members; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; required for replication of Brome mosaic virus in S. cerevisiae Gene:YIL166C(YIL166C_p)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Putative protein with similarity to the allantoate permease (Dal5p) subfamily of the major facilitator superfamily; mRNA expression is elevated by sulfur limitation; YIL166C is a non-essential gene Gene:YJR005C-A(YJR005C-A_p)|FD-Score:-4.01|P-value:2.99E-5||SGD DESC:Putative protein of unknown function; originally identified as a syntenic homolog of an <i>Ashbya gossypii</i> gene; YJR005C-A has a paralog, YGR169C-A, that arose from the whole genome duplication Gene:YKE2(YLR200W)|FD-Score:5.98|P-value:1.11E-9||SGD DESC:Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin Gene:YKL050C(YKL050C)|FD-Score:-3.2|P-value:6.89E-4||SGD DESC:Protein of unknown function; the YKL050W protein is a target of the SCFCdc4 ubiquitin ligase complex and YKL050W transcription is regulated by Azf1p Gene:YLR123C(YLR123C_d)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR122C; contains characteristic aminoacyl-tRNA motif Gene:YML119W(YML119W_p)|FD-Score:-3.34|P-value:4.16E-4||SGD DESC:Putative protein of unknown function; YML119W is not an essential gene; potential Cdc28p substrate Gene:YMR001C-A(YMR001C-A_p)|FD-Score:-5.06|P-value:2.07E-7||SGD DESC:Putative protein of unknown function Gene:YMR247W-A(YMR247W-A_p)|FD-Score:-4.42|P-value:4.92E-6||SGD DESC:Putative protein of unknown function Gene:YNL143C(YNL143C_p)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YNL296W(YNL296W_d)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion adversely affects sporulation; deletion mutant exhibits synthetic phenotype under expression of mutant huntingtin fragment, but gene does not have human ortholog Gene:YNR005C(YNR005C_d)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR040W(YNR040W_p)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR012W(YOR012W_p)|FD-Score:3.23|P-value:6.30E-4||SGD DESC:Putative protein of unknown function Gene:YPL109C(YPL109C_p)|FD-Score:3.11|P-value:9.28E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:ADK2(YER170W)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Mitochondrial adenylate kinase, catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background Gene:ALG3(YBL082C)|FD-Score:-4.48|P-value:3.73E-6||SGD DESC:Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins Gene:ANP1(YEL036C)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:APJ1(YNL077W)|FD-Score:5.77|P-value:4.04E-9||SGD DESC:Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:ATG15(YCR068W)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway Gene:ATP11(YNL315C)|FD-Score:-3.79|P-value:7.40E-5||SGD DESC:Molecular chaperone, required for the assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase Gene:BIO2(YGR286C)|FD-Score:-4.02|P-value:2.86E-5||SGD DESC:Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant Gene:COX19(YLL018C-A)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:CRC1(YOR100C)|FD-Score:-3.25|P-value:5.72E-4||SGD DESC:Mitochondrial inner membrane carnitine transporter, required for carnitine-dependent transport of acetyl-CoA from peroxisomes to mitochondria during fatty acid beta-oxidation Gene:CRR1(YLR213C)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Putative glycoside hydrolase of the spore wall envelope; required for normal spore wall assembly, possibly for cross-linking between the glucan and chitosan layers; expressed during sporulation Gene:CUP2(YGL166W)|FD-Score:-3.53|P-value:2.11E-4||SGD DESC:Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; CUP2 has a paralog, HAA1, that arose from the whole genome duplication Gene:DFG5(YMR238W)|FD-Score:6.15|P-value:3.87E-10||SGD DESC:Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p Gene:DUR3(YHL016C)|FD-Score:-3.41|P-value:3.19E-4||SGD DESC:Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway Gene:DUS4(YLR405W)|FD-Score:3.16|P-value:7.91E-4||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p Gene:EEB1(YPL095C)|FD-Score:-4.51|P-value:3.25E-6||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification; EEB1 has a paralog, EHT1, that arose from the whole genome duplication Gene:ENT5(YDR153C)|FD-Score:3.18|P-value:7.40E-4||SGD DESC:Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p, clathrin adaptor complex AP-1, and clathrin Gene:EXG1(YLR300W)|FD-Score:-3.81|P-value:6.88E-5||SGD DESC:Major exo-1,3-beta-glucanase of the cell wall, involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes Gene:GAT2(YMR136W)|FD-Score:-5.57|P-value:1.30E-8||SGD DESC:Protein containing GATA family zinc finger motifs; similar to Gln3p and Dal80p; expression repressed by leucine Gene:GIS2(YNL255C)|FD-Score:-3.83|P-value:6.45E-5||SGD DESC:Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 Gene:INP1(YMR204C)|FD-Score:-3.99|P-value:3.33E-5||SGD DESC:Peripheral membrane protein of peroxisomes involved in peroxisomal inheritance; recruitment to peroxisomes is mediated by interaction with Pex3p at the peroxisomal membrane Gene:JSN1(YJR091C)|FD-Score:3.21|P-value:6.61E-4||SGD DESC:Member of the Puf family of RNA-binding proteins, interacts with mRNAs encoding membrane-associated proteins; involved in localizing the Arp2/3 complex to mitochondria; overexpression causes increased sensitivity to benomyl Gene:MFM1(YPL060W)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p Gene:MRPL20(YKR085C)|FD-Score:3.17|P-value:7.58E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSS18(YPR134W)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions Gene:MTM1(YGR257C)|FD-Score:5.1|P-value:1.71E-7||SGD DESC:Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor Gene:OSW5(YMR148W)|FD-Score:-3.32|P-value:4.56E-4||SGD DESC:Protein of unknown function that may play a role in spore wall assembly; predicted to contain an N-terminal transmembrane domain; osw5 null mutant spores exhibit increased spore wall permeability and sensitivity to beta-glucanase digestion Gene:PBS2(YJL128C)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PUT4(YOR348C)|FD-Score:-3.51|P-value:2.21E-4||SGD DESC:Proline permease, required for high-affinity transport of proline; also transports the toxic proline analog azetidine-2-carboxylate (AzC); PUT4 transcription is repressed in ammonia-grown cells Gene:RAD5(YLR032W)|FD-Score:4.82|P-value:7.14E-7||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:REX4(YOL080C)|FD-Score:-3.37|P-value:3.72E-4||SGD DESC:Putative RNA exonuclease possibly involved in pre-rRNA processing and ribosome assembly Gene:RNH202(YDR279W)|FD-Score:4.55|P-value:2.68E-6||SGD DESC:Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS2 that causes Aicardi-Goutieres syndrome Gene:RPL19A(YBR084C-A)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPS12(YOR369C)|FD-Score:-3.26|P-value:5.66E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S12, no bacterial homolog Gene:RPS29A(YLR388W)|FD-Score:4.5|P-value:3.38E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication Gene:RRT14(YIL127C_p)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Putative protein of unknown function; identified in a screen for mutants with decreased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis Gene:RRT16(YNL105W_d)|FD-Score:-3.58|P-value:1.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene INP52; identified in a screen for mutants with decreased levels of rDNA transcription Gene:RSM28(YDR494W)|FD-Score:-3.52|P-value:2.18E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation Gene:RTC6(YPL183W-A)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:RTN1(YDR233C)|FD-Score:-3.1|P-value:9.67E-4||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; mutant overexpressing RTN1 shows increase in tubular ER; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; more abundant than Rtn2p; member of the RTNLA subfamily; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; RTN1 has a paralog, RTN2, that arose from the whole genome duplication Gene:RUD3(YOR216C)|FD-Score:-5.18|P-value:1.13E-7||SGD DESC:Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1 Gene:SAE3(YHR079C-A)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Mei5p; proposed to be an assembly factor for Dmc1p Gene:SFL1(YOR140W)|FD-Score:-3.38|P-value:3.60E-4||SGD DESC:Transcriptional repressor and activator; involved in repression of flocculation-related genes, and activation of stress responsive genes; negatively regulated by cAMP-dependent protein kinase A subunit Tpk2p Gene:SPO71(YDR104C)|FD-Score:-3.38|P-value:3.62E-4||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation, in particular for the proper size of the prospore membrane (PSM); interacts with SPO1; dispensable for both nuclear divisions during meiosis; contains two PH domains Gene:SPO77(YLR341W)|FD-Score:3.32|P-value:4.44E-4||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis Gene:SSB1(YDL229W)|FD-Score:-3.87|P-value:5.46E-5||SGD DESC:Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in folding of newly-made polypeptide chains; member of the HSP70 family; interacts with phosphatase subunit Reg1p; SSB1 has a paralog, SSB2, that arose from the whole genome duplication Gene:TEC1(YBR083W)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Transcription factor targeting filamentation genes and Ty1 expression; Ste12p activation of most filamentation gene promoters depends on Tec1p and Tec1p transcriptional activity is dependent on its association with Ste12p; binds to TCS elements upstream of filamentation genes, which are regulated by Tec1p/Ste12p/Dig1p complex; competes with Dig2p for binding to Ste12p/Dig1p; positive regulator of chronological life span; TEA/ATTS DNA-binding domain family member Gene:WAR1(YML076C)|FD-Score:-6.88|P-value:3.09E-12||SGD DESC:Homodimeric Zn2Cys6 zinc finger transcription factor; binds to a weak acid response element to induce transcription of PDR12 and FUN34, encoding an acid transporter and a putative ammonia transporter, respectively Gene:YBR232C(YBR232C_d)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBT1(YLL048C)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters Gene:YER087C-A(YER087C-A_d)|FD-Score:-3.51|P-value:2.28E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; overlaps the uncharacterized gene YER087W, a putative tRNA synthetase Gene:YGR001C(YGR001C_p)|FD-Score:-3.59|P-value:1.67E-4||SGD DESC:Putative protein of unknown function with similarity to methyltransferase family members; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; required for replication of Brome mosaic virus in S. cerevisiae Gene:YIL166C(YIL166C_p)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Putative protein with similarity to the allantoate permease (Dal5p) subfamily of the major facilitator superfamily; mRNA expression is elevated by sulfur limitation; YIL166C is a non-essential gene Gene:YJR005C-A(YJR005C-A_p)|FD-Score:-4.01|P-value:2.99E-5||SGD DESC:Putative protein of unknown function; originally identified as a syntenic homolog of an <i>Ashbya gossypii</i> gene; YJR005C-A has a paralog, YGR169C-A, that arose from the whole genome duplication Gene:YKE2(YLR200W)|FD-Score:5.98|P-value:1.11E-9||SGD DESC:Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin Gene:YKL050C(YKL050C)|FD-Score:-3.2|P-value:6.89E-4||SGD DESC:Protein of unknown function; the YKL050W protein is a target of the SCFCdc4 ubiquitin ligase complex and YKL050W transcription is regulated by Azf1p Gene:YLR123C(YLR123C_d)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR122C; contains characteristic aminoacyl-tRNA motif Gene:YML119W(YML119W_p)|FD-Score:-3.34|P-value:4.16E-4||SGD DESC:Putative protein of unknown function; YML119W is not an essential gene; potential Cdc28p substrate Gene:YMR001C-A(YMR001C-A_p)|FD-Score:-5.06|P-value:2.07E-7||SGD DESC:Putative protein of unknown function Gene:YMR247W-A(YMR247W-A_p)|FD-Score:-4.42|P-value:4.92E-6||SGD DESC:Putative protein of unknown function Gene:YNL143C(YNL143C_p)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YNL296W(YNL296W_d)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion adversely affects sporulation; deletion mutant exhibits synthetic phenotype under expression of mutant huntingtin fragment, but gene does not have human ortholog Gene:YNR005C(YNR005C_d)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR040W(YNR040W_p)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR012W(YOR012W_p)|FD-Score:3.23|P-value:6.30E-4||SGD DESC:Putative protein of unknown function Gene:YPL109C(YPL109C_p)|FD-Score:3.11|P-value:9.28E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL008W5.551.44E-80.69APC11Catalytic core subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; contains a RING-H2 domain that is required for activity
YCL054W5.238.65E-80.69SPB1AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants
YHR019C5.091.83E-70.69DED81Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA
YFR002W4.993.08E-70.69NIC96Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p)
YOR074C4.631.85E-60.69CDC21Thymidylate synthase, required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S
YLR010C3.944.05E-50.32TEN1Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p
YDR472W3.621.49E-40.09TRS31One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
YKL145W3.532.09E-40.01RPT1One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance
YFR042W3.512.21E-40.03KEG1Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability
YBL014C3.482.48E-40.14RRN6Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
YLR314C3.344.20E-40.03CDC3Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YDR013W3.314.71E-40.23PSF1Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
YBL097W3.080.001040.04BRN1Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission
YPL160W3.040.001200.02CDC60Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA
YKR022C3.020.001270.02NTR2Essential protein that forms a dimer with Ntr1p; also forms a trimer, with Ntr2p and the DExD/H-box RNA helicase Prp43p, that is involved in spliceosome disassembly

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR238W6.153.87E-10DFG5Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p
YLR200W5.981.11E-9YKE2Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin
YNL077W5.774.04E-9APJ1Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress
YGR257C5.101.71E-7MTM1Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor
YLR032W4.827.14E-7RAD5DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress
YDR279W4.552.68E-6RNH202Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS2 that causes Aicardi-Goutieres syndrome
YLR388W4.503.38E-6RPS29AProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication
YHR079C-A4.221.22E-5SAE3Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Mei5p; proposed to be an assembly factor for Dmc1p
YLL048C4.211.29E-5YBT1Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters
YPL060W4.191.40E-5MFM1Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p
YNR005C_d3.701.07E-4YNR005C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YNL296W_d3.691.13E-4YNL296W_dDubious open reading frame unlikely to encode a functional protein; deletion adversely affects sporulation; deletion mutant exhibits synthetic phenotype under expression of mutant huntingtin fragment, but gene does not have human ortholog
YNR040W_p3.671.21E-4YNR040W_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YBR232C_d3.671.22E-4YBR232C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YCR068W3.621.46E-4ATG15Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway

GO enrichment analysis for SGTC_2922
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0791.50E-9SGTC_13071187-1599 12.2 μMChemDiv (Drug-like library)15384710.0655738PDR1
0.0741.36E-8SGTC_245methotrexate 289.6 μMMiscellaneous1269410.0941176
0.0656.01E-7SGTC_28177995342 71.4 μMChembridge (Drug-like library)188799750.134328
0.0612.98E-6SGTC_2779methotrexate 400.0 μMMiscellaneous1269410.0941176
0.0571.32E-5SGTC_32069114350 49.5 μMChembridge (Drug-like library)170872430.25
0.0561.96E-5SGTC_29237992654 58.1 μMChembridge (Drug-like library)29851260.241379Golgi
0.0543.89E-5SGTC_13181280-3875 124.0 μMChemDiv (Drug-like library)3732450.108696
0.0534.06E-5SGTC_29909074105 71.4 μMChembridge (Drug-like library)65020010.0833333
0.0526.36E-5SGTC_30979117246 49.5 μMChembridge (Drug-like library)164238920.12987
0.0517.62E-5SGTC_1913914-0123 66.5 μMChemDiv (Drug-like library)31444120.13636460S ribosome export
0.0518.08E-5SGTC_31929110362 49.5 μMChembridge (Drug-like library)166494800.152778mitochondrial processes
0.0519.62E-5SGTC_29249010168 62.8 μMChembridge (Drug-like library)29939290.216667cell wall signaling
0.0501.26E-4SGTC_14801070-0044 41.2 μMChemDiv (Drug-like library)31249870.1
0.0501.27E-4SGTC_23729071633 200.0 μMChembridge (Fragment library)213213660.25
0.0501.30E-4SGTC_20975376186 200.0 μMChembridge (Fragment library)7427550.166667

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2977901119771.43 μM0.4468092704722Chembridge (Drug-like library)279.3050463.41914
SGTC_2826799254119.48 μM0.4166672985066Chembridge (Drug-like library)297.6845063.52514
SGTC_12971068-011114.1 μM0.387755767057ChemDiv (Drug-like library)312.214244.33712
SGTC_850868-0259160.06 μM0.34693983173ChemDiv (Drug-like library)256.256682.5431360S ribosome export
SGTC_3174495-0002170 μM0.34545515991618ChemDiv (Drug-like library)351.26143.07613
SGTC_13431487-0095224 μM0.3396234560101ChemDiv (Drug-like library)327.827864.5812
SGTC_2844901010325.97 μM0.3333332480525Chembridge (Drug-like library)327.321494.01916
SGTC_3171910319249.47 μM0.33333313018434Chembridge (Drug-like library)269.338263.50512
SGTC_3207911416349.47 μM0.333333849233Chembridge (Drug-like library)269.338263.43812
SGTC_14474239-081681.9 μM0.3278691341554ChemDiv (Drug-like library)367.4597433.41814