9044753

ethyl 2-[[2-(4-ethylpiperazin-1-yl)acetyl]amino]-5,6,7,8-tetrahydro-4H-cyclohepta[b]thiophene-3-carboxylate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2937
Screen concentration 11.3 μM
Source Chembridge (Drug-like library)
PubChem CID 6465530
SMILES CCN1CCN(CC1)CC(=O)NC2=C(C3=C(S2)CCCCC3)C(=O)OCC
Standardized SMILES CCOC(=O)c1c2CCCCCc2sc1NC(=O)CN3CCN(CC)CC3
Molecular weight 393.5434
ALogP 3.09
H-bond donor count 1
H-bond acceptor count 6
Response signature 60S ribosome export

Pool Growth Kinetics
% growth inhibition (Het. pool) 10.84
% growth inhibition (Hom. pool) 6.62


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6465530
Download HIP data (tab-delimited text)  (excel)
Gene:CCT3(YJL014W)|FD-Score:-3.13|P-value:8.76E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC23(YHR166C)|FD-Score:-3.48|P-value:2.53E-4|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CIA2(YHR122W)|FD-Score:3.49|P-value:2.46E-4|Clearance:0.08||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human FAM96B Gene:GPI17(YDR434W)|FD-Score:3.26|P-value:5.65E-4|Clearance:0.25||SGD DESC:Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-S homolog Gene:INO80(YGL150C)|FD-Score:3.72|P-value:9.97E-5|Clearance:0.04||SGD DESC:ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription Gene:IQG1(YPL242C)|FD-Score:3.34|P-value:4.25E-4|Clearance:0||SGD DESC:Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:IRA1(YBR140C)|FD-Score:-4.92|P-value:4.32E-7|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:LSG1(YGL099W)|FD-Score:16.9|P-value:1.54E-64|Clearance:10.3||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:LSM4(YER112W)|FD-Score:3.33|P-value:4.31E-4|Clearance:0.07||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:MCM10(YIL150C)|FD-Score:3.97|P-value:3.62E-5|Clearance:0.25||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:MRS6(YOR370C)|FD-Score:5.13|P-value:1.43E-7|Clearance:0.27||SGD DESC:Rab escort protein; forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); sequence similarity to mammalian choroideraemia gene; relative distribution to the nucleus increases upon DNA replication stress Gene:MTR10(YOR160W)|FD-Score:-3.89|P-value:4.93E-5|Clearance:0||SGD DESC:Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:NEO1(YIL048W)|FD-Score:6.63|P-value:1.73E-11|Clearance:0.87||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:NMD3(YHR170W)|FD-Score:5.76|P-value:4.31E-9|Clearance:0.39||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:RPF1(YHR088W)|FD-Score:3.68|P-value:1.17E-4|Clearance:0.19||SGD DESC:Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA Gene:RPS15(YOL040C)|FD-Score:-3.73|P-value:9.39E-5|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RPT2(YDL007W)|FD-Score:-3.22|P-value:6.50E-4|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle Gene:SEC14(YMR079W)|FD-Score:-4.99|P-value:3.04E-7|Clearance:0||SGD DESC:Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs Gene:SEC26(YDR238C)|FD-Score:4.86|P-value:5.86E-7|Clearance:0.88||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SMX2(YFL017W-A)|FD-Score:3.41|P-value:3.28E-4|Clearance:0.07||SGD DESC:Core Sm protein Sm G; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx3p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm G Gene:SPP2(YOR148C)|FD-Score:3.98|P-value:3.49E-5|Clearance:0||SGD DESC:Essential protein that promotes the first step of splicing and is required for the final stages of spliceosome maturation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction Gene:SRB6(YBR253W)|FD-Score:3.26|P-value:5.49E-4|Clearance:0.01||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:SSY5(YJL156C)|FD-Score:-3.28|P-value:5.17E-4|Clearance:0||SGD DESC:Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p Gene:SWD2(YKL018W)|FD-Score:-4.08|P-value:2.26E-5|Clearance:0||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination Gene:SWI1(YPL016W)|FD-Score:3.97|P-value:3.53E-5|Clearance:0.01||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer Gene:TAF10(YDR167W)|FD-Score:-3.63|P-value:1.41E-4|Clearance:0||SGD DESC:Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:YGR265W(YGR265W_d)|FD-Score:5.37|P-value:3.94E-8|Clearance:0.24||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase Gene:CCT3(YJL014W)|FD-Score:-3.13|P-value:8.76E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC23(YHR166C)|FD-Score:-3.48|P-value:2.53E-4|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CIA2(YHR122W)|FD-Score:3.49|P-value:2.46E-4|Clearance:0.08||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human FAM96B Gene:GPI17(YDR434W)|FD-Score:3.26|P-value:5.65E-4|Clearance:0.25||SGD DESC:Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-S homolog Gene:INO80(YGL150C)|FD-Score:3.72|P-value:9.97E-5|Clearance:0.04||SGD DESC:ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription Gene:IQG1(YPL242C)|FD-Score:3.34|P-value:4.25E-4|Clearance:0||SGD DESC:Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:IRA1(YBR140C)|FD-Score:-4.92|P-value:4.32E-7|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:LSG1(YGL099W)|FD-Score:16.9|P-value:1.54E-64|Clearance:10.3||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:LSM4(YER112W)|FD-Score:3.33|P-value:4.31E-4|Clearance:0.07||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:MCM10(YIL150C)|FD-Score:3.97|P-value:3.62E-5|Clearance:0.25||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:MRS6(YOR370C)|FD-Score:5.13|P-value:1.43E-7|Clearance:0.27||SGD DESC:Rab escort protein; forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); sequence similarity to mammalian choroideraemia gene; relative distribution to the nucleus increases upon DNA replication stress Gene:MTR10(YOR160W)|FD-Score:-3.89|P-value:4.93E-5|Clearance:0||SGD DESC:Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:NEO1(YIL048W)|FD-Score:6.63|P-value:1.73E-11|Clearance:0.87||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:NMD3(YHR170W)|FD-Score:5.76|P-value:4.31E-9|Clearance:0.39||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:RPF1(YHR088W)|FD-Score:3.68|P-value:1.17E-4|Clearance:0.19||SGD DESC:Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA Gene:RPS15(YOL040C)|FD-Score:-3.73|P-value:9.39E-5|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RPT2(YDL007W)|FD-Score:-3.22|P-value:6.50E-4|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle Gene:SEC14(YMR079W)|FD-Score:-4.99|P-value:3.04E-7|Clearance:0||SGD DESC:Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs Gene:SEC26(YDR238C)|FD-Score:4.86|P-value:5.86E-7|Clearance:0.88||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SMX2(YFL017W-A)|FD-Score:3.41|P-value:3.28E-4|Clearance:0.07||SGD DESC:Core Sm protein Sm G; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx3p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm G Gene:SPP2(YOR148C)|FD-Score:3.98|P-value:3.49E-5|Clearance:0||SGD DESC:Essential protein that promotes the first step of splicing and is required for the final stages of spliceosome maturation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction Gene:SRB6(YBR253W)|FD-Score:3.26|P-value:5.49E-4|Clearance:0.01||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:SSY5(YJL156C)|FD-Score:-3.28|P-value:5.17E-4|Clearance:0||SGD DESC:Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p Gene:SWD2(YKL018W)|FD-Score:-4.08|P-value:2.26E-5|Clearance:0||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination Gene:SWI1(YPL016W)|FD-Score:3.97|P-value:3.53E-5|Clearance:0.01||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer Gene:TAF10(YDR167W)|FD-Score:-3.63|P-value:1.41E-4|Clearance:0||SGD DESC:Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:YGR265W(YGR265W_d)|FD-Score:5.37|P-value:3.94E-8|Clearance:0.24||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6465530
Download HOP data (tab-delimited text)  (excel)
Gene:ADE5,7(YGL234W)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities Gene:AIM22(YJL046W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Putative lipoate-protein ligase, required along with Lip2 and Lip5 for lipoylation of Lat1p and Kgd2p; similar to E. coli LplA; null mutant displays reduced frequency of mitochondrial genome loss Gene:ASH1(YKL185W)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Zinc-finger inhibitor of HO transcription; mRNA is localized and translated in the distal tip of anaphase cells, resulting in accumulation of Ash1p in daughter cell nuclei and inhibition of HO expression; potential Cdc28p substrate Gene:ATF1(YOR377W)|FD-Score:-3.38|P-value:3.63E-4||SGD DESC:Alcohol acetyltransferase; responsible for the major part of volatile acetate ester production during fermentation; main enzyme involved in terpenyl acetate synthesis; potential roles in lipid and sterol metabolism Gene:ATG23(YLR431C)|FD-Score:3.83|P-value:6.44E-5||SGD DESC:Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p Gene:BDF1(YLR399C)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:BNA5(YLR231C)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Kynureninase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:BOP3(YNL042W)|FD-Score:-3.47|P-value:2.62E-4||SGD DESC:Protein of unknown function, potential Cdc28p substrate; overproduction confers resistance to methylmercury Gene:BUD3(YCL014W)|FD-Score:-3.84|P-value:6.27E-5||SGD DESC:Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding Gene:COQ5(YML110C)|FD-Score:6.16|P-value:3.54E-10||SGD DESC:2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes Gene:CPT1(YNL130C)|FD-Score:-3.28|P-value:5.12E-4||SGD DESC:Cholinephosphotransferase; required for phosphatidylcholine biosynthesis and for inositol-dependent regulation of EPT1 transcription; CPT1 has a paralog, EPT1, that arose from the whole genome duplication Gene:CUE5(YOR042W)|FD-Score:6.97|P-value:1.63E-12||SGD DESC:Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:CUR1(YPR158W)|FD-Score:4.32|P-value:7.87E-6||SGD DESC:Sorting factor, central regulator of spatial protein quality control; physically and functionally interacts with chaperones to promote sorting and deposition of misfolded proteins into cytosolic compartments; involved in destabilization of [URE3] prions; CUR1 has a paralog, BTN2, that arose from the whole genome duplication Gene:DAT1(YML113W)|FD-Score:-4.19|P-value:1.42E-5||SGD DESC:DNA binding protein that recognizes oligo(dA).oligo(dT) tracts; Arg side chain in its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is required for DNA-binding; not essential for viability Gene:DID4(YKL002W)|FD-Score:3.78|P-value:7.72E-5||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:DSS4(YPR017C)|FD-Score:3.91|P-value:4.58E-5||SGD DESC:Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol Gene:ECM33(YBR078W)|FD-Score:4.35|P-value:6.85E-6||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:EMC6(YLL014W)|FD-Score:-3.28|P-value:5.22E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm F33D4.7/EMC-6, fly CG11781, human TMEM93 Gene:ERG2(YMR202W)|FD-Score:7.17|P-value:3.77E-13||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FPR4(YLR449W)|FD-Score:-3.6|P-value:1.57E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase) (proline isomerase) localized to the nucleus; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones Gene:GAL4(YPL248C)|FD-Score:-5.14|P-value:1.40E-7||SGD DESC:DNA-binding transcription factor required for the activation of the GAL genes in response to galactose; repressed by Gal80p and activated by Gal3p Gene:GET4(YOR164C)|FD-Score:4.43|P-value:4.79E-6||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Mdy2p; highly conserved across species and homologous to human gene C7orf20 Gene:GIS2(YNL255C)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 Gene:GOT1(YMR292W)|FD-Score:-3.16|P-value:7.87E-4||SGD DESC:Homodimeric protein that is packaged into COPII vesicles and cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition Gene:GTB1(YDR221W)|FD-Score:4.73|P-value:1.13E-6||SGD DESC:Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p; involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER; relocalizes from ER to cytoplasm upon DNA replication stress Gene:HFA1(YMR207C)|FD-Score:-4.1|P-value:2.10E-5||SGD DESC:Mitochondrial acetyl-coenzyme A carboxylase; catalyzes the production of malonyl-CoA in mitochondrial fatty acid biosynthesis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:HIS7(YBR248C)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Imidazole glycerol phosphate synthase (glutamine amidotransferase:cyclase), catalyzes the fifth step of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor Gene:HMG1(YML075C)|FD-Score:3.93|P-value:4.19E-5||SGD DESC:HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress Gene:HOS4(YIL112W)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Subunit of the Set3 complex, which is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate Gene:HSM3(YBR272C)|FD-Score:-5.74|P-value:4.83E-9||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation Gene:HSP31(YDR533C)|FD-Score:3.29|P-value:5.00E-4||SGD DESC:Possible chaperone and cysteine protease; has similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress Gene:HST1(YOL068C)|FD-Score:5.26|P-value:7.12E-8||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:IES6(YEL044W)|FD-Score:3.25|P-value:5.74E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes Gene:INO1(YJL153C)|FD-Score:-3.09|P-value:9.85E-4||SGD DESC:Inositol-3-phosphate synthase, involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element Gene:IRC13(YOR235W_d)|FD-Score:-4.92|P-value:4.25E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:ISU2(YOR226C)|FD-Score:3.16|P-value:7.98E-4||SGD DESC:Protein required for synthesis of iron-sulfur proteins; localized to the mitochondrial matrix; performs a scaffolding function in mitochondria during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; isu1 isu2 double mutant is inviable; protein abundance increases in response to DNA replication stress; evolutionarily conserved Gene:KSS1(YGR040W)|FD-Score:4.62|P-value:1.94E-6||SGD DESC:Mitogen-activated protein kinase (MAPK) involved in signal transduction pathways that control filamentous growth and pheromone response; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains Gene:LST4(YKL176C)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MNT3(YIL014W)|FD-Score:5|P-value:2.84E-7||SGD DESC:Alpha-1,3-mannosyltransferase, adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation Gene:MRPL1(YDR116C)|FD-Score:3.78|P-value:7.72E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS8(YMR158W)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MVB12(YGR206W)|FD-Score:3.96|P-value:3.73E-5||SGD DESC:ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin Gene:NMA111(YNL123W)|FD-Score:5.48|P-value:2.14E-8||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:NUP53(YMR153W)|FD-Score:-3.18|P-value:7.42E-4||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport; involved in regulation of transcription and mitosis; induces membrane tubulation, which may contribute to nuclear pore assembly; NUP53 has a paralog, ASM4, that arose from the whole genome duplication Gene:OPI6(YDL096C_d)|FD-Score:-3.31|P-value:4.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential Gene:PBP4(YDL053C)|FD-Score:3.25|P-value:5.86E-4||SGD DESC:Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress Gene:PEP3(YLR148W)|FD-Score:4.89|P-value:5.16E-7||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PEP7(YDR323C)|FD-Score:4.67|P-value:1.50E-6||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PIN4(YBL051C)|FD-Score:6.1|P-value:5.38E-10||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:POM152(YMR129W)|FD-Score:-3.32|P-value:4.50E-4||SGD DESC:Glycoprotein subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NUP210 Gene:PRO2(YOR323C)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RFM1(YOR279C)|FD-Score:6.29|P-value:1.54E-10||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RMD6(YEL072W)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Protein required for sporulation Gene:RPL36B(YPL249C-A)|FD-Score:4.5|P-value:3.35E-6||SGD DESC:Ribosomal 60S subunit protein L36B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36B has a paralog, RPL36A, that arose from the whole genome duplication Gene:RPL8A(YHL033C)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication Gene:RPS4A(YJR145C)|FD-Score:3.85|P-value:5.80E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication Gene:RRT7(YLL030C_d)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:RTR2(YDR066C_p)|FD-Score:-3.6|P-value:1.58E-4||SGD DESC:Protein of unknown function; exhibits genetic interactions with Rtr1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; RTR2 has a paralog, RTR1, that arose from the whole genome duplication Gene:SAC1(YKL212W)|FD-Score:4.73|P-value:1.15E-6||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SCP1(YOR367W)|FD-Score:3.9|P-value:4.78E-5||SGD DESC:Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin Gene:SCY1(YGL083W)|FD-Score:-3.8|P-value:7.28E-5||SGD DESC:Putative kinase, suppressor of GTPase mutant, similar to bovine rhodopsin kinase Gene:SEC72(YLR292C)|FD-Score:3.14|P-value:8.46E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SGF29(YCL010C)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation Gene:SHM2(YLR058C)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis Gene:SKN7(YHR206W)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SNF7(YLR025W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SNQ2(YDR011W)|FD-Score:4.93|P-value:4.06E-7||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SPL2(YHR136C)|FD-Score:7.38|P-value:7.79E-14||SGD DESC:Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation; overproduction suppresses a plc1 null mutation; GFP-fusion protein localizes to the cytoplasm Gene:SSA4(YER103W)|FD-Score:5.77|P-value:3.85E-9||SGD DESC:Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation; SSA4 has a paralog, SSA3, that arose from the whole genome duplication Gene:SSM4(YIL030C)|FD-Score:3.24|P-value:6.00E-4||SGD DESC:Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation Gene:SUB1(YMR039C)|FD-Score:-5.39|P-value:3.62E-8||SGD DESC:Transcriptional coactivator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; protein abundance increases in response to DNA replication stress Gene:TMS1(YDR105C)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:Vacuolar membrane protein of unknown function that is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance Gene:TOP1(YOL006C)|FD-Score:4.09|P-value:2.12E-5||SGD DESC:Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination Gene:TRP3(YKL211C)|FD-Score:-3.4|P-value:3.38E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UBI4(YLL039C)|FD-Score:-3.24|P-value:5.99E-4||SGD DESC:Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats; protein abundance increases in response to DNA replication stress Gene:UBX4(YMR067C)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress Gene:URM1(YIL008W)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VMA11(YPL234C)|FD-Score:3.11|P-value:9.36E-4||SGD DESC:Vacuolar ATPase V0 domain subunit c', involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen Gene:VPS20(YMR077C)|FD-Score:3.88|P-value:5.14E-5||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS74(YDR372C)|FD-Score:5.7|P-value:6.00E-9||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:YBR300C(YBR300C_d)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene Gene:YCK1(YHR135C)|FD-Score:3.79|P-value:7.65E-5||SGD DESC:Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck2p in morphogenesis, proper septin assembly, endocytic trafficking; YCK1 has a paralog, YCK2, that arose from the whole genome duplication Gene:YCL012C(YCL012C)|FD-Score:-3.75|P-value:8.72E-5||SGD DESC:Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene Gene:YCL022C(YCL022C_d)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene KCC4/YCL024W Gene:YDR415C(YDR415C_p)|FD-Score:5.43|P-value:2.82E-8||SGD DESC:Putative aminopeptidase Gene:YEL045C(YEL045C_d)|FD-Score:-4.06|P-value:2.49E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YER097W(YER097W_d)|FD-Score:4.42|P-value:5.00E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR001C(YGR001C_p)|FD-Score:-4.17|P-value:1.54E-5||SGD DESC:Putative protein of unknown function with similarity to methyltransferase family members; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; required for replication of Brome mosaic virus in S. cerevisiae Gene:YGR018C(YGR018C_p)|FD-Score:-3.57|P-value:1.79E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the uncharacterized ORF YGR017W Gene:YGR066C(YGR066C_p)|FD-Score:3.26|P-value:5.53E-4||SGD DESC:Putative protein of unknown function Gene:YGR291C(YGR291C_d)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHR180W(YHR180W_d)|FD-Score:-4.15|P-value:1.68E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL025C(YIL025C_d)|FD-Score:3.1|P-value:9.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR020W(YJR020W_d)|FD-Score:3.96|P-value:3.75E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR151W-A(YJR151W-A_p)|FD-Score:-4.74|P-value:1.05E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR; predicted to have a role in transcription based on computational guilt by association analysis Gene:YKL069W(YKL069W)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Methionine-R-sulfoxide reductase; reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress; relative distribution to the nucleus increases upon DNA replication stress Gene:YKL091C(YKL091C)|FD-Score:5.34|P-value:4.68E-8||SGD DESC:Putative homolog of Sec14p, which is a phosphatidylinositol/phosphatidylcholine transfer protein involved in lipid metabolism; localizes to the nucleus; contains a CRAL/TRIO domain and binds several lipids in a large-scale study Gene:YKR018C(YKR018C_p)|FD-Score:-3.65|P-value:1.31E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication Gene:YLL056C(YLL056C_p)|FD-Score:-3.71|P-value:1.06E-4||SGD DESC:Putative protein of unknown function, transcription is activated by paralogous transcription factors Yrm1p and Yrr1p and genes involved in pleiotropic drug resistance (PDR); expression is induced in cells treated with the mycotoxin patulin Gene:YLR307C-A(YLR307C-A_p)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:Putative protein of unknown function Gene:YMR124W(YMR124W_p)|FD-Score:4.3|P-value:8.67E-6||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization Gene:YNL226W(YNL226W_d)|FD-Score:8.89|P-value:2.98E-19||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C Gene:YOL036W(YOL036W_p)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Protein of unknown function; potential Cdc28p substrate; YOL036W has a paralog, YIR016W, that arose from the whole genome duplication Gene:YOL159C(YOL159C)|FD-Score:-3.1|P-value:9.69E-4||SGD DESC:Soluble protein of unknown function; deletion mutants are viable and have elevated levels of Ty1 retrotransposition and Ty1 cDNA Gene:YPL034W(YPL034W_p)|FD-Score:-3.25|P-value:5.87E-4||SGD DESC:Putative protein of unknown function; YPL034W is not essential gene Gene:YPL038W-A(YPL038W-A_p)|FD-Score:4.8|P-value:8.05E-7||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPR014C(YPR014C_d)|FD-Score:-4.02|P-value:2.87E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene Gene:YSP1(YHR155W)|FD-Score:-3.09|P-value:9.92E-4||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication Gene:ADE5,7(YGL234W)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities Gene:AIM22(YJL046W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Putative lipoate-protein ligase, required along with Lip2 and Lip5 for lipoylation of Lat1p and Kgd2p; similar to E. coli LplA; null mutant displays reduced frequency of mitochondrial genome loss Gene:ASH1(YKL185W)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Zinc-finger inhibitor of HO transcription; mRNA is localized and translated in the distal tip of anaphase cells, resulting in accumulation of Ash1p in daughter cell nuclei and inhibition of HO expression; potential Cdc28p substrate Gene:ATF1(YOR377W)|FD-Score:-3.38|P-value:3.63E-4||SGD DESC:Alcohol acetyltransferase; responsible for the major part of volatile acetate ester production during fermentation; main enzyme involved in terpenyl acetate synthesis; potential roles in lipid and sterol metabolism Gene:ATG23(YLR431C)|FD-Score:3.83|P-value:6.44E-5||SGD DESC:Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p Gene:BDF1(YLR399C)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:BNA5(YLR231C)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Kynureninase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:BOP3(YNL042W)|FD-Score:-3.47|P-value:2.62E-4||SGD DESC:Protein of unknown function, potential Cdc28p substrate; overproduction confers resistance to methylmercury Gene:BUD3(YCL014W)|FD-Score:-3.84|P-value:6.27E-5||SGD DESC:Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding Gene:COQ5(YML110C)|FD-Score:6.16|P-value:3.54E-10||SGD DESC:2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes Gene:CPT1(YNL130C)|FD-Score:-3.28|P-value:5.12E-4||SGD DESC:Cholinephosphotransferase; required for phosphatidylcholine biosynthesis and for inositol-dependent regulation of EPT1 transcription; CPT1 has a paralog, EPT1, that arose from the whole genome duplication Gene:CUE5(YOR042W)|FD-Score:6.97|P-value:1.63E-12||SGD DESC:Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:CUR1(YPR158W)|FD-Score:4.32|P-value:7.87E-6||SGD DESC:Sorting factor, central regulator of spatial protein quality control; physically and functionally interacts with chaperones to promote sorting and deposition of misfolded proteins into cytosolic compartments; involved in destabilization of [URE3] prions; CUR1 has a paralog, BTN2, that arose from the whole genome duplication Gene:DAT1(YML113W)|FD-Score:-4.19|P-value:1.42E-5||SGD DESC:DNA binding protein that recognizes oligo(dA).oligo(dT) tracts; Arg side chain in its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is required for DNA-binding; not essential for viability Gene:DID4(YKL002W)|FD-Score:3.78|P-value:7.72E-5||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:DSS4(YPR017C)|FD-Score:3.91|P-value:4.58E-5||SGD DESC:Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol Gene:ECM33(YBR078W)|FD-Score:4.35|P-value:6.85E-6||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:EMC6(YLL014W)|FD-Score:-3.28|P-value:5.22E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm F33D4.7/EMC-6, fly CG11781, human TMEM93 Gene:ERG2(YMR202W)|FD-Score:7.17|P-value:3.77E-13||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FPR4(YLR449W)|FD-Score:-3.6|P-value:1.57E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase) (proline isomerase) localized to the nucleus; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones Gene:GAL4(YPL248C)|FD-Score:-5.14|P-value:1.40E-7||SGD DESC:DNA-binding transcription factor required for the activation of the GAL genes in response to galactose; repressed by Gal80p and activated by Gal3p Gene:GET4(YOR164C)|FD-Score:4.43|P-value:4.79E-6||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Mdy2p; highly conserved across species and homologous to human gene C7orf20 Gene:GIS2(YNL255C)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 Gene:GOT1(YMR292W)|FD-Score:-3.16|P-value:7.87E-4||SGD DESC:Homodimeric protein that is packaged into COPII vesicles and cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition Gene:GTB1(YDR221W)|FD-Score:4.73|P-value:1.13E-6||SGD DESC:Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p; involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER; relocalizes from ER to cytoplasm upon DNA replication stress Gene:HFA1(YMR207C)|FD-Score:-4.1|P-value:2.10E-5||SGD DESC:Mitochondrial acetyl-coenzyme A carboxylase; catalyzes the production of malonyl-CoA in mitochondrial fatty acid biosynthesis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:HIS7(YBR248C)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Imidazole glycerol phosphate synthase (glutamine amidotransferase:cyclase), catalyzes the fifth step of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor Gene:HMG1(YML075C)|FD-Score:3.93|P-value:4.19E-5||SGD DESC:HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress Gene:HOS4(YIL112W)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Subunit of the Set3 complex, which is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate Gene:HSM3(YBR272C)|FD-Score:-5.74|P-value:4.83E-9||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation Gene:HSP31(YDR533C)|FD-Score:3.29|P-value:5.00E-4||SGD DESC:Possible chaperone and cysteine protease; has similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress Gene:HST1(YOL068C)|FD-Score:5.26|P-value:7.12E-8||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:IES6(YEL044W)|FD-Score:3.25|P-value:5.74E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes Gene:INO1(YJL153C)|FD-Score:-3.09|P-value:9.85E-4||SGD DESC:Inositol-3-phosphate synthase, involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element Gene:IRC13(YOR235W_d)|FD-Score:-4.92|P-value:4.25E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:ISU2(YOR226C)|FD-Score:3.16|P-value:7.98E-4||SGD DESC:Protein required for synthesis of iron-sulfur proteins; localized to the mitochondrial matrix; performs a scaffolding function in mitochondria during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; isu1 isu2 double mutant is inviable; protein abundance increases in response to DNA replication stress; evolutionarily conserved Gene:KSS1(YGR040W)|FD-Score:4.62|P-value:1.94E-6||SGD DESC:Mitogen-activated protein kinase (MAPK) involved in signal transduction pathways that control filamentous growth and pheromone response; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains Gene:LST4(YKL176C)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MNT3(YIL014W)|FD-Score:5|P-value:2.84E-7||SGD DESC:Alpha-1,3-mannosyltransferase, adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation Gene:MRPL1(YDR116C)|FD-Score:3.78|P-value:7.72E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS8(YMR158W)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MVB12(YGR206W)|FD-Score:3.96|P-value:3.73E-5||SGD DESC:ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin Gene:NMA111(YNL123W)|FD-Score:5.48|P-value:2.14E-8||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:NUP53(YMR153W)|FD-Score:-3.18|P-value:7.42E-4||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport; involved in regulation of transcription and mitosis; induces membrane tubulation, which may contribute to nuclear pore assembly; NUP53 has a paralog, ASM4, that arose from the whole genome duplication Gene:OPI6(YDL096C_d)|FD-Score:-3.31|P-value:4.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential Gene:PBP4(YDL053C)|FD-Score:3.25|P-value:5.86E-4||SGD DESC:Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress Gene:PEP3(YLR148W)|FD-Score:4.89|P-value:5.16E-7||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PEP7(YDR323C)|FD-Score:4.67|P-value:1.50E-6||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PIN4(YBL051C)|FD-Score:6.1|P-value:5.38E-10||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:POM152(YMR129W)|FD-Score:-3.32|P-value:4.50E-4||SGD DESC:Glycoprotein subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NUP210 Gene:PRO2(YOR323C)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RFM1(YOR279C)|FD-Score:6.29|P-value:1.54E-10||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RMD6(YEL072W)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Protein required for sporulation Gene:RPL36B(YPL249C-A)|FD-Score:4.5|P-value:3.35E-6||SGD DESC:Ribosomal 60S subunit protein L36B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36B has a paralog, RPL36A, that arose from the whole genome duplication Gene:RPL8A(YHL033C)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication Gene:RPS4A(YJR145C)|FD-Score:3.85|P-value:5.80E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication Gene:RRT7(YLL030C_d)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:RTR2(YDR066C_p)|FD-Score:-3.6|P-value:1.58E-4||SGD DESC:Protein of unknown function; exhibits genetic interactions with Rtr1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; RTR2 has a paralog, RTR1, that arose from the whole genome duplication Gene:SAC1(YKL212W)|FD-Score:4.73|P-value:1.15E-6||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SCP1(YOR367W)|FD-Score:3.9|P-value:4.78E-5||SGD DESC:Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin Gene:SCY1(YGL083W)|FD-Score:-3.8|P-value:7.28E-5||SGD DESC:Putative kinase, suppressor of GTPase mutant, similar to bovine rhodopsin kinase Gene:SEC72(YLR292C)|FD-Score:3.14|P-value:8.46E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SGF29(YCL010C)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation Gene:SHM2(YLR058C)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis Gene:SKN7(YHR206W)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SNF7(YLR025W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SNQ2(YDR011W)|FD-Score:4.93|P-value:4.06E-7||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SPL2(YHR136C)|FD-Score:7.38|P-value:7.79E-14||SGD DESC:Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation; overproduction suppresses a plc1 null mutation; GFP-fusion protein localizes to the cytoplasm Gene:SSA4(YER103W)|FD-Score:5.77|P-value:3.85E-9||SGD DESC:Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation; SSA4 has a paralog, SSA3, that arose from the whole genome duplication Gene:SSM4(YIL030C)|FD-Score:3.24|P-value:6.00E-4||SGD DESC:Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation Gene:SUB1(YMR039C)|FD-Score:-5.39|P-value:3.62E-8||SGD DESC:Transcriptional coactivator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; protein abundance increases in response to DNA replication stress Gene:TMS1(YDR105C)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:Vacuolar membrane protein of unknown function that is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance Gene:TOP1(YOL006C)|FD-Score:4.09|P-value:2.12E-5||SGD DESC:Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination Gene:TRP3(YKL211C)|FD-Score:-3.4|P-value:3.38E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UBI4(YLL039C)|FD-Score:-3.24|P-value:5.99E-4||SGD DESC:Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats; protein abundance increases in response to DNA replication stress Gene:UBX4(YMR067C)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress Gene:URM1(YIL008W)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VMA11(YPL234C)|FD-Score:3.11|P-value:9.36E-4||SGD DESC:Vacuolar ATPase V0 domain subunit c', involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen Gene:VPS20(YMR077C)|FD-Score:3.88|P-value:5.14E-5||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS74(YDR372C)|FD-Score:5.7|P-value:6.00E-9||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:YBR300C(YBR300C_d)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene Gene:YCK1(YHR135C)|FD-Score:3.79|P-value:7.65E-5||SGD DESC:Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck2p in morphogenesis, proper septin assembly, endocytic trafficking; YCK1 has a paralog, YCK2, that arose from the whole genome duplication Gene:YCL012C(YCL012C)|FD-Score:-3.75|P-value:8.72E-5||SGD DESC:Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene Gene:YCL022C(YCL022C_d)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene KCC4/YCL024W Gene:YDR415C(YDR415C_p)|FD-Score:5.43|P-value:2.82E-8||SGD DESC:Putative aminopeptidase Gene:YEL045C(YEL045C_d)|FD-Score:-4.06|P-value:2.49E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YER097W(YER097W_d)|FD-Score:4.42|P-value:5.00E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR001C(YGR001C_p)|FD-Score:-4.17|P-value:1.54E-5||SGD DESC:Putative protein of unknown function with similarity to methyltransferase family members; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; required for replication of Brome mosaic virus in S. cerevisiae Gene:YGR018C(YGR018C_p)|FD-Score:-3.57|P-value:1.79E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the uncharacterized ORF YGR017W Gene:YGR066C(YGR066C_p)|FD-Score:3.26|P-value:5.53E-4||SGD DESC:Putative protein of unknown function Gene:YGR291C(YGR291C_d)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHR180W(YHR180W_d)|FD-Score:-4.15|P-value:1.68E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL025C(YIL025C_d)|FD-Score:3.1|P-value:9.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR020W(YJR020W_d)|FD-Score:3.96|P-value:3.75E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR151W-A(YJR151W-A_p)|FD-Score:-4.74|P-value:1.05E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR; predicted to have a role in transcription based on computational guilt by association analysis Gene:YKL069W(YKL069W)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Methionine-R-sulfoxide reductase; reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress; relative distribution to the nucleus increases upon DNA replication stress Gene:YKL091C(YKL091C)|FD-Score:5.34|P-value:4.68E-8||SGD DESC:Putative homolog of Sec14p, which is a phosphatidylinositol/phosphatidylcholine transfer protein involved in lipid metabolism; localizes to the nucleus; contains a CRAL/TRIO domain and binds several lipids in a large-scale study Gene:YKR018C(YKR018C_p)|FD-Score:-3.65|P-value:1.31E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication Gene:YLL056C(YLL056C_p)|FD-Score:-3.71|P-value:1.06E-4||SGD DESC:Putative protein of unknown function, transcription is activated by paralogous transcription factors Yrm1p and Yrr1p and genes involved in pleiotropic drug resistance (PDR); expression is induced in cells treated with the mycotoxin patulin Gene:YLR307C-A(YLR307C-A_p)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:Putative protein of unknown function Gene:YMR124W(YMR124W_p)|FD-Score:4.3|P-value:8.67E-6||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization Gene:YNL226W(YNL226W_d)|FD-Score:8.89|P-value:2.98E-19||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C Gene:YOL036W(YOL036W_p)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Protein of unknown function; potential Cdc28p substrate; YOL036W has a paralog, YIR016W, that arose from the whole genome duplication Gene:YOL159C(YOL159C)|FD-Score:-3.1|P-value:9.69E-4||SGD DESC:Soluble protein of unknown function; deletion mutants are viable and have elevated levels of Ty1 retrotransposition and Ty1 cDNA Gene:YPL034W(YPL034W_p)|FD-Score:-3.25|P-value:5.87E-4||SGD DESC:Putative protein of unknown function; YPL034W is not essential gene Gene:YPL038W-A(YPL038W-A_p)|FD-Score:4.8|P-value:8.05E-7||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPR014C(YPR014C_d)|FD-Score:-4.02|P-value:2.87E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene Gene:YSP1(YHR155W)|FD-Score:-3.09|P-value:9.92E-4||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YGL099W16.901.54E-6410.30LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YIL048W6.631.73E-110.87NEO1Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus
YHR170W5.764.31E-90.39NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YGR265W_d5.373.94E-80.24YGR265W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase
YOR370C5.131.43E-70.27MRS6Rab escort protein; forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); sequence similarity to mammalian choroideraemia gene; relative distribution to the nucleus increases upon DNA replication stress
YDR238C4.865.86E-70.88SEC26Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP)
YOR148C3.983.49E-50.00SPP2Essential protein that promotes the first step of splicing and is required for the final stages of spliceosome maturation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction
YPL016W3.973.53E-50.01SWI1Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer
YIL150C3.973.62E-50.25MCM10Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins
YGL150C3.729.97E-50.04INO80ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription
YHR088W3.681.17E-40.19RPF1Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA
YHR122W3.492.46E-40.08CIA2Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human FAM96B
YFL017W-A3.413.28E-40.07SMX2Core Sm protein Sm G; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx3p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm G
YPL242C3.344.25E-40.00IQG1Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress
YER112W3.334.31E-40.07LSM4Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNL226W_d8.892.98E-19YNL226W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C
YHR136C7.387.79E-14SPL2Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation; overproduction suppresses a plc1 null mutation; GFP-fusion protein localizes to the cytoplasm
YMR202W7.173.77E-13ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YOR042W6.971.63E-12CUE5Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
YOR279C6.291.54E-10RFM1DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance
YML110C6.163.54E-10COQ52-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes
YBL051C6.105.38E-10PIN4Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage
YER103W5.773.85E-9SSA4Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation; SSA4 has a paralog, SSA3, that arose from the whole genome duplication
YDR372C5.706.00E-9VPS74Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33
YNL123W5.482.14E-8NMA111Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases
YDR415C_p5.432.82E-8YDR415C_pPutative aminopeptidase
YKL091C5.344.68E-8YKL091CPutative homolog of Sec14p, which is a phosphatidylinositol/phosphatidylcholine transfer protein involved in lipid metabolism; localizes to the nucleus; contains a CRAL/TRIO domain and binds several lipids in a large-scale study
YOL068C5.267.12E-8HST1NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance
YIL014W5.002.84E-7MNT3Alpha-1,3-mannosyltransferase, adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation
YDR011W4.934.06E-7SNQ2Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species

GO enrichment analysis for SGTC_2937
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2393.38E-77SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.060606160S ribosome export
0.2311.23E-72SGTC_970535-0701 19.1 μMChemDiv (Drug-like library)28320130.15584460S ribosome export
0.2313.43E-72SGTC_1070indatraline 8.8 μMNIH Clinical Collection103144720.052631660S ribosome export
0.2151.58E-62SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.024096460S ribosome export
0.2041.09E-56SGTC_8103825-7150 143.0 μMChemDiv (Drug-like library)223304110.044943860S ribosome export
0.1851.43E-46SGTC_1775st081588 35.2 μMTimTec (Natural product derivative library)14264430.10227360S ribosome export
0.1756.73E-42SGTC_1913914-0123 66.5 μMChemDiv (Drug-like library)31444120.035294160S ribosome export
0.1734.20E-41SGTC_1958st076513 41.9 μMTimTec (Natural product derivative library)7321220.024691460S ribosome export
0.1701.68E-39SGTC_32809140961 49.5 μMChembridge (Drug-like library)49012260.057471360S ribosome export
0.1702.69E-39SGTC_2734clemastine 41.6 μMMiscellaneous269870.0987654ERG2
0.1631.40E-36SGTC_8660495-0101 262.0 μMChemDiv (Drug-like library)6356940.096385560S ribosome export
0.1611.32E-35SGTC_12470448-0043 5.8 μMChemDiv (Drug-like library)30890130.0512821ERG2
0.1604.90E-35SGTC_22067254556 147.0 μMChembridge (Fragment library)43989050.16901460S ribosome export
0.1591.06E-34SGTC_2742clothiapine 36.3 μMMiscellaneous163510.060241NEO1
0.1553.61E-33SGTC_850868-0259 160.1 μMChemDiv (Drug-like library)831730.11267660S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1704st02880570.8 μM0.6538462876101TimTec (Natural product derivative library)282.358622.5724
SGTC_9751335-004628.5 μM0.46031716486740ChemDiv (Drug-like library)374.250142.67915RPP1 & pyrimidine depletion
SGTC_1756st04155835.5 μM0.43753955792TimTec (Natural product derivative library)564.649284.77928
SGTC_309k408-00511.48 μM0.4324323640463ChemDiv (Drug-like library)505.650043.32218
SGTC_3216912971649.47 μM0.4285718056934Chembridge (Drug-like library)310.368721.40815RPP1 & pyrimidine depletion
SGTC_3145908551649.47 μM0.37096825236195Chembridge (Drug-like library)306.466143.79903fatty acid desaturase (OLE1)
SGTC_9133487-1245167 μM0.3432844016153ChemDiv (Drug-like library)349.831844.53424
SGTC_10134112-3890241 μM0.342857705171ChemDiv (Drug-like library)331.386183.39725TSC3-RPN4
SGTC_2833900131545.46 μM0.3428572989378Chembridge (Drug-like library)358.411522.55425amide catabolism
SGTC_8034488-1585147 μM0.338235747204ChemDiv (Drug-like library)315.386783.924