9052777

2-chloro-N-(3-chlorophenyl)-4-fluorobenzamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2942
Screen concentration 62.2 μM
Source Chembridge (Drug-like library)
PubChem CID 6469503
SMILES C1=CC(=CC(=C1)Cl)NC(=O)C2=C(C=C(C=C2)F)Cl
Standardized SMILES Fc1ccc(C(=O)Nc2cccc(Cl)c2)c(Cl)c1
Molecular weight 284.1131
ALogP 4.15
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 13.9
% growth inhibition (Hom. pool) 9.7


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6469503
Download HIP data (tab-delimited text)  (excel)
Gene:ALG7(YBR243C)|FD-Score:4.07|P-value:2.31E-5|Clearance:0.18||SGD DESC:UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin Gene:BCP1(YDR361C)|FD-Score:4.56|P-value:2.57E-6|Clearance:0.03||SGD DESC:Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport Gene:CDC14(YFR028C)|FD-Score:3.24|P-value:5.88E-4|Clearance:0.02||SGD DESC:Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR and Mitotic Exit Network in anaphase, enabling it to act on key substrates to effect a decrease in CDK/B-cyclin activity and mitotic exit; required for meiosis I spindle disassembly; released from nucleolus upon entry into anaphase I of meiosis, resequestered in metaphase II, then released again upon entry into anaphase II; maintained in nucleolus by Cdc55p in early meiosis Gene:DUT1(YBR252W)|FD-Score:3.23|P-value:6.26E-4|Clearance:0.16||SGD DESC:deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate Gene:GCD6(YDR211W)|FD-Score:-3.85|P-value:6.01E-5|Clearance:0||SGD DESC:Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress Gene:GLN4(YOR168W)|FD-Score:3.43|P-value:2.96E-4|Clearance:0.08||SGD DESC:Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions Gene:INN1(YNL152W)|FD-Score:3.28|P-value:5.24E-4|Clearance:0||SGD DESC:Essential protein that associates with contractile actomyosin ring; required for ingression of the plasma membrane into the bud neck during cytokinesis; C2 domain, a membrane targeting module, is required for function; activates chitin synthase activity of Chs2p during cytokinesis Gene:KRS1(YDR037W)|FD-Score:7.6|P-value:1.47E-14|Clearance:1.66||SGD DESC:Lysyl-tRNA synthetase Gene:MAK5(YBR142W)|FD-Score:5.94|P-value:1.43E-9|Clearance:0.91||SGD DESC:Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits Gene:MRS6(YOR370C)|FD-Score:3.27|P-value:5.32E-4|Clearance:0.03||SGD DESC:Rab escort protein; forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); sequence similarity to mammalian choroideraemia gene; relative distribution to the nucleus increases upon DNA replication stress Gene:PDC2(YDR081C)|FD-Score:4.53|P-value:2.95E-6|Clearance:0.46||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:PUP2(YGR253C)|FD-Score:3.58|P-value:1.73E-4|Clearance:0.1||SGD DESC:Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta Gene:RVB2(YPL235W)|FD-Score:3.33|P-value:4.35E-4|Clearance:0.05||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SHQ1(YIL104C)|FD-Score:3.9|P-value:4.91E-5|Clearance:0.05||SGD DESC:Chaperone protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones Gene:SNU56(YDR240C)|FD-Score:3.36|P-value:3.91E-4|Clearance:0.03||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex Gene:SRM1(YGL097W)|FD-Score:3.48|P-value:2.48E-4|Clearance:0.05||SGD DESC:Nucleotide exchange factor for Gsp1p, localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p Gene:SUP35(YDR172W)|FD-Score:-4.11|P-value:1.98E-5|Clearance:0||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:TSC10(YBR265W)|FD-Score:4.84|P-value:6.58E-7|Clearance:0.28||SGD DESC:3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family Gene:TUB2(YFL037W)|FD-Score:3.84|P-value:6.09E-5|Clearance:0.26||SGD DESC:Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria Gene:YJR023C(YJR023C_d)|FD-Score:5.03|P-value:2.46E-7|Clearance:0.19||SGD DESC:Putative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing Gene:ALG7(YBR243C)|FD-Score:4.07|P-value:2.31E-5|Clearance:0.18||SGD DESC:UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin Gene:BCP1(YDR361C)|FD-Score:4.56|P-value:2.57E-6|Clearance:0.03||SGD DESC:Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport Gene:CDC14(YFR028C)|FD-Score:3.24|P-value:5.88E-4|Clearance:0.02||SGD DESC:Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR and Mitotic Exit Network in anaphase, enabling it to act on key substrates to effect a decrease in CDK/B-cyclin activity and mitotic exit; required for meiosis I spindle disassembly; released from nucleolus upon entry into anaphase I of meiosis, resequestered in metaphase II, then released again upon entry into anaphase II; maintained in nucleolus by Cdc55p in early meiosis Gene:DUT1(YBR252W)|FD-Score:3.23|P-value:6.26E-4|Clearance:0.16||SGD DESC:deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate Gene:GCD6(YDR211W)|FD-Score:-3.85|P-value:6.01E-5|Clearance:0||SGD DESC:Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress Gene:GLN4(YOR168W)|FD-Score:3.43|P-value:2.96E-4|Clearance:0.08||SGD DESC:Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions Gene:INN1(YNL152W)|FD-Score:3.28|P-value:5.24E-4|Clearance:0||SGD DESC:Essential protein that associates with contractile actomyosin ring; required for ingression of the plasma membrane into the bud neck during cytokinesis; C2 domain, a membrane targeting module, is required for function; activates chitin synthase activity of Chs2p during cytokinesis Gene:KRS1(YDR037W)|FD-Score:7.6|P-value:1.47E-14|Clearance:1.66||SGD DESC:Lysyl-tRNA synthetase Gene:MAK5(YBR142W)|FD-Score:5.94|P-value:1.43E-9|Clearance:0.91||SGD DESC:Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits Gene:MRS6(YOR370C)|FD-Score:3.27|P-value:5.32E-4|Clearance:0.03||SGD DESC:Rab escort protein; forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); sequence similarity to mammalian choroideraemia gene; relative distribution to the nucleus increases upon DNA replication stress Gene:PDC2(YDR081C)|FD-Score:4.53|P-value:2.95E-6|Clearance:0.46||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:PUP2(YGR253C)|FD-Score:3.58|P-value:1.73E-4|Clearance:0.1||SGD DESC:Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta Gene:RVB2(YPL235W)|FD-Score:3.33|P-value:4.35E-4|Clearance:0.05||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SHQ1(YIL104C)|FD-Score:3.9|P-value:4.91E-5|Clearance:0.05||SGD DESC:Chaperone protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones Gene:SNU56(YDR240C)|FD-Score:3.36|P-value:3.91E-4|Clearance:0.03||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex Gene:SRM1(YGL097W)|FD-Score:3.48|P-value:2.48E-4|Clearance:0.05||SGD DESC:Nucleotide exchange factor for Gsp1p, localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p Gene:SUP35(YDR172W)|FD-Score:-4.11|P-value:1.98E-5|Clearance:0||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:TSC10(YBR265W)|FD-Score:4.84|P-value:6.58E-7|Clearance:0.28||SGD DESC:3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family Gene:TUB2(YFL037W)|FD-Score:3.84|P-value:6.09E-5|Clearance:0.26||SGD DESC:Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria Gene:YJR023C(YJR023C_d)|FD-Score:5.03|P-value:2.46E-7|Clearance:0.19||SGD DESC:Putative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6469503
Download HOP data (tab-delimited text)  (excel)
Gene:AKL1(YBR059C)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Ser-Thr protein kinase, member (with Ark1p and Prk1p) of the Ark kinase family; involved in endocytosis and actin cytoskeleton organization Gene:APE1(YKL103C)|FD-Score:4.93|P-value:4.01E-7||SGD DESC:Vacuolar aminopeptidase yscI; zinc metalloproteinase that belongs to the peptidase family M18; often used as a marker protein in studies of autophagy and cytosol to vacuole targeting (CVT) pathway; protein increases in abundance and relative distribution to cytoplasmic foci increases upon DNA replication stress Gene:ARL3(YPL051W)|FD-Score:5.3|P-value:5.71E-8||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ARP8(YOR141C)|FD-Score:-3.32|P-value:4.45E-4||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; has mRNA binding activity Gene:ATG3(YNR007C)|FD-Score:5.05|P-value:2.16E-7||SGD DESC:E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p Gene:BAT2(YJR148W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Cytosolic branched-chain amino acid (BCAA) aminotransferase, preferentially involved in BCAA catabolism; homolog of murine ECA39; highly expressed during stationary phase and repressed during logarithmic phase Gene:BCS1(YDR375C)|FD-Score:4.41|P-value:5.06E-6||SGD DESC:Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases Gene:BOP3(YNL042W)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Protein of unknown function, potential Cdc28p substrate; overproduction confers resistance to methylmercury Gene:CBS2(YDR197W)|FD-Score:5.23|P-value:8.34E-8||SGD DESC:Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader Gene:CCH1(YGR217W)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together Gene:CDC73(YLR418C)|FD-Score:4.67|P-value:1.47E-6||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CMK2(YOL016C)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication Gene:COG8(YML071C)|FD-Score:4.52|P-value:3.05E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CPR5(YDR304C)|FD-Score:-3.47|P-value:2.61E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER Gene:CUR1(YPR158W)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:Sorting factor, central regulator of spatial protein quality control; physically and functionally interacts with chaperones to promote sorting and deposition of misfolded proteins into cytosolic compartments; involved in destabilization of [URE3] prions; CUR1 has a paralog, BTN2, that arose from the whole genome duplication Gene:CUS2(YNL286W)|FD-Score:3.92|P-value:4.40E-5||SGD DESC:Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) Gene:CYB5(YNL111C)|FD-Score:-3.48|P-value:2.53E-4||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:DAL5(YJR152W)|FD-Score:3.72|P-value:1.02E-4||SGD DESC:Allantoate permease; ureidosuccinate permease; also transports dipeptides, though with lower affinity than for allantoate and ureidosuccinate; expression is constitutive but sensitive to nitrogen catabolite repression Gene:DAL80(YKR034W)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Gzf3p; DAL80 has a paralog, GZF3, that arose from the whole genome duplication Gene:DEG1(YFL001W)|FD-Score:3.75|P-value:8.71E-5||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability Gene:ECM7(YLR443W)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Non-essential putative integral membrane protein with a role in calcium uptake; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency Gene:ERV15(YBR210W)|FD-Score:-3.29|P-value:5.08E-4||SGD DESC:Protein involved in export of proteins from the endoplasmic reticulum; ERV15 has a paralog, ERV14, that arose from the whole genome duplication Gene:ESL2(YKR096W)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Protein of unknown function that interacts with Pex14p; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; predicted to contain a PINc domain Gene:FUI1(YBL042C)|FD-Score:3.15|P-value:8.09E-4||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:HIS6(YIL020C)|FD-Score:-3.59|P-value:1.66E-4||SGD DESC:Phosphoribosyl-5-amino-1-phosphoribosyl-4-imidazolecarboxiamide isomerase, catalyzes the fourth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:IRS4(YKR019C)|FD-Score:8.17|P-value:1.58E-16||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISN1(YOR155C)|FD-Score:4.31|P-value:8.16E-6||SGD DESC:Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase, catalyzes the breakdown of IMP to inosine, does not show similarity to known 5'-nucleotidases from other organisms Gene:JEM1(YJL073W)|FD-Score:3.96|P-value:3.82E-5||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:KIN82(YCR091W)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Putative serine/threonine protein kinase implicated in the regulation of phospholipid asymmetry through the activation of phospholipid translocases (flippases) Lem3p-Dnf1p/Dnf2p; similar to Fpk1p Gene:MFA1(YDR461W)|FD-Score:-3.38|P-value:3.67E-4||SGD DESC:Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA2 Gene:MSC1(YML128C)|FD-Score:-4.29|P-value:9.12E-6||SGD DESC:Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated Gene:MTO1(YGL236C)|FD-Score:4.36|P-value:6.49E-6||SGD DESC:Mitochondrial protein, forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants Gene:PAN3(YKL025C)|FD-Score:8.31|P-value:4.79E-17||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PET309(YLR067C)|FD-Score:4.48|P-value:3.81E-6||SGD DESC:Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains seven pentatricopeptide repeats (PPRs) Gene:PFA5(YDR459C)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Palmitoyltransferase with autoacylation activity; likely functions in pathway(s) outside Ras; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain Gene:PHO90(YJL198W)|FD-Score:4.67|P-value:1.54E-6||SGD DESC:Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication Gene:PIF1(YML061C)|FD-Score:-3.77|P-value:8.23E-5||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:PKH2(YOL100W)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p Gene:PMR1(YGL167C)|FD-Score:3.93|P-value:4.32E-5||SGD DESC:High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease Gene:PMT5(YDL093W)|FD-Score:3.77|P-value:8.30E-5||SGD DESC:Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt3p, can instead interact with Pmt2p in some conditions; target for new antifungals Gene:PSY2(YNL201C)|FD-Score:-10.1|P-value:2.25E-24||SGD DESC:Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 Gene:QDR1(YIL120W)|FD-Score:-4.65|P-value:1.62E-6||SGD DESC:Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for resistance to quinidine, ketoconazole, fluconazole, and barban; QDR1 has a paralog, AQR1, that arose from the whole genome duplication Gene:RAD33(YML011C)|FD-Score:4.93|P-value:4.13E-7||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:RAD51(YER095W)|FD-Score:-4.78|P-value:8.68E-7||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RCN2(YOR220W_p)|FD-Score:5.08|P-value:1.89E-7||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; phosphorylated in response to alpha factor; protein abundance increases in response to DNA replication stress Gene:RHO5(YNL180C)|FD-Score:3.84|P-value:6.18E-5||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely involved in protein kinase C (Pkc1p)-dependent signal transduction pathway that controls cell integrity Gene:RKM1(YPL208W)|FD-Score:4.31|P-value:8.21E-6||SGD DESC:SET-domain lysine-N-methyltransferase, catalyzes the formation of dimethyllysine residues on the large ribsomal subunit proteins L23a (Rpl23Ap and Rpl23Bp) and L18 (Rps18Ap and Rps18Bp) Gene:RMA1(YKL132C)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Putative dihydrofolate synthetase; has similarity to Fol3p and to E. coli folylpolyglutamate synthetase/dihydrofolate synthetase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RPL13A(YDL082W)|FD-Score:-4.25|P-value:1.08E-5||SGD DESC:Ribosomal 60S subunit protein L13A; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13A has a paralog, RPL13B, that arose from the whole genome duplication Gene:RPL6A(YML073C)|FD-Score:-4.14|P-value:1.74E-5||SGD DESC:Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication Gene:RPS21B(YJL136C)|FD-Score:-4.15|P-value:1.69E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication Gene:RSM27(YGR215W)|FD-Score:4.48|P-value:3.82E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RTT102(YGR275W)|FD-Score:3.73|P-value:9.50E-5||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; suggested role in chromosome maintenance; possible weak regulator of Ty1 transposition; protein abundance increases in response to DNA replication stress Gene:SDS3(YIL084C)|FD-Score:3.2|P-value:6.96E-4||SGD DESC:Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes Gene:SED4(YCR067C)|FD-Score:-3.64|P-value:1.35E-4||SGD DESC:Integral endoplasmic reticulum membrane protein that stimulates Sar1p GTPase activity; involved in COPII vesicle budding through disassociation of coat proteins from membranes onto liposomes; binds Sec16p; similar to Sec12p Gene:SGF73(YGL066W)|FD-Score:3.94|P-value:4.06E-5||SGD DESC:SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay Gene:SNQ2(YDR011W)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SNZ2(YNL333W)|FD-Score:4.65|P-value:1.68E-6||SGD DESC:Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p Gene:SSD1(YDR293C)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSN2(YDR443C)|FD-Score:-3.37|P-value:3.81E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:SVF1(YDR346C)|FD-Score:3.23|P-value:6.13E-4||SGD DESC:Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance Gene:TAL1(YLR354C)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate Gene:TAT2(YOL020W)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TCB2(YNL087W)|FD-Score:4.51|P-value:3.28E-6||SGD DESC:ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains 3 calcium and lipid binding domains; mRNA is targeted to the bud Gene:TIF1(YKR059W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF1 has a paralog, TIF2, that arose from the whole genome duplication Gene:TIR3(YIL011W)|FD-Score:-4.26|P-value:1.01E-5||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth Gene:TLG2(YOL018C)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TOK1(YJL093C)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Outward-rectifier potassium channel of the plasma membrane with two pore domains in tandem, each of which forms a functional channel permeable to potassium; carboxy tail functions to prevent inner gate closures; target of K1 toxin Gene:TPO1(YLL028W)|FD-Score:-4.17|P-value:1.54E-5||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane Gene:TRM3(YDL112W)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs Gene:TRS85(YDR108W)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:TVP15(YDR100W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p Gene:UBC5(YDR059C)|FD-Score:4.05|P-value:2.55E-5||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication Gene:UBP6(YFR010W)|FD-Score:4.43|P-value:4.81E-6||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:URA1(YKL216W)|FD-Score:3.74|P-value:9.37E-5||SGD DESC:Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid Gene:VID24(YBR105C)|FD-Score:5.24|P-value:8.16E-8||SGD DESC:GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles Gene:VPS8(YAL002W)|FD-Score:-4.26|P-value:1.03E-5||SGD DESC:Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif Gene:YBR174C(YBR174C_d)|FD-Score:3.85|P-value:5.89E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YBR182C-A(YBR182C-A_p)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR196C-A(YBR196C-A_p)|FD-Score:5.68|P-value:6.57E-9||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR008C(YDR008C_d)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR115W(YDR115W_p)|FD-Score:3.73|P-value:9.39E-5||SGD DESC:Putative mitochondrial ribosomal protein of the large subunit; similar to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins; protein increases in abundance and relocalizes to the plasma membrane upon DNA replication stress Gene:YDR199W(YDR199W_d)|FD-Score:4.81|P-value:7.73E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity Gene:YER076C(YER076C_p)|FD-Score:3.81|P-value:7.04E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization Gene:YGL108C(YGL108C_p)|FD-Score:-4.96|P-value:3.61E-7||SGD DESC:Protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress Gene:YGL242C(YGL242C_p)|FD-Score:4.35|P-value:6.82E-6||SGD DESC:Putative protein of unknown function; deletion mutant is viable Gene:YJR037W(YJR037W_d)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene HUL4/YJR036C; deletion mutant has decreased spore survival in Drosophila feces Gene:YJR128W(YJR128W_d)|FD-Score:4.36|P-value:6.53E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RSF2 Gene:YLL007C(YLL007C_p)|FD-Score:4.61|P-value:2.02E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YLR253W(YLR253W_p)|FD-Score:3.33|P-value:4.38E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; transcription is periodic during the metabolic cycle; YLR253W is not an essential gene Gene:YLR294C(YLR294C_d)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ATP14 Gene:YML122C(YML122C_d)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL108C(YNL108C_p)|FD-Score:9.75|P-value:9.38E-23||SGD DESC:Putative protein of unknown function; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication Gene:YOL024W(YOL024W_p)|FD-Score:3.99|P-value:3.30E-5||SGD DESC:Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site; YOL024W has a paralog, IGD1, that arose from the whole genome duplication Gene:YPL014W(YPL014W_p)|FD-Score:3.2|P-value:6.80E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YPL107W(YPL107W_p)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YPL107W is not an essential gene Gene:YSC84(YHR016C)|FD-Score:6.21|P-value:2.61E-10||SGD DESC:Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication Gene:AKL1(YBR059C)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Ser-Thr protein kinase, member (with Ark1p and Prk1p) of the Ark kinase family; involved in endocytosis and actin cytoskeleton organization Gene:APE1(YKL103C)|FD-Score:4.93|P-value:4.01E-7||SGD DESC:Vacuolar aminopeptidase yscI; zinc metalloproteinase that belongs to the peptidase family M18; often used as a marker protein in studies of autophagy and cytosol to vacuole targeting (CVT) pathway; protein increases in abundance and relative distribution to cytoplasmic foci increases upon DNA replication stress Gene:ARL3(YPL051W)|FD-Score:5.3|P-value:5.71E-8||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ARP8(YOR141C)|FD-Score:-3.32|P-value:4.45E-4||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; has mRNA binding activity Gene:ATG3(YNR007C)|FD-Score:5.05|P-value:2.16E-7||SGD DESC:E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p Gene:BAT2(YJR148W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Cytosolic branched-chain amino acid (BCAA) aminotransferase, preferentially involved in BCAA catabolism; homolog of murine ECA39; highly expressed during stationary phase and repressed during logarithmic phase Gene:BCS1(YDR375C)|FD-Score:4.41|P-value:5.06E-6||SGD DESC:Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases Gene:BOP3(YNL042W)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Protein of unknown function, potential Cdc28p substrate; overproduction confers resistance to methylmercury Gene:CBS2(YDR197W)|FD-Score:5.23|P-value:8.34E-8||SGD DESC:Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader Gene:CCH1(YGR217W)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together Gene:CDC73(YLR418C)|FD-Score:4.67|P-value:1.47E-6||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CMK2(YOL016C)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication Gene:COG8(YML071C)|FD-Score:4.52|P-value:3.05E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CPR5(YDR304C)|FD-Score:-3.47|P-value:2.61E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER Gene:CUR1(YPR158W)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:Sorting factor, central regulator of spatial protein quality control; physically and functionally interacts with chaperones to promote sorting and deposition of misfolded proteins into cytosolic compartments; involved in destabilization of [URE3] prions; CUR1 has a paralog, BTN2, that arose from the whole genome duplication Gene:CUS2(YNL286W)|FD-Score:3.92|P-value:4.40E-5||SGD DESC:Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) Gene:CYB5(YNL111C)|FD-Score:-3.48|P-value:2.53E-4||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:DAL5(YJR152W)|FD-Score:3.72|P-value:1.02E-4||SGD DESC:Allantoate permease; ureidosuccinate permease; also transports dipeptides, though with lower affinity than for allantoate and ureidosuccinate; expression is constitutive but sensitive to nitrogen catabolite repression Gene:DAL80(YKR034W)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Gzf3p; DAL80 has a paralog, GZF3, that arose from the whole genome duplication Gene:DEG1(YFL001W)|FD-Score:3.75|P-value:8.71E-5||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability Gene:ECM7(YLR443W)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Non-essential putative integral membrane protein with a role in calcium uptake; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency Gene:ERV15(YBR210W)|FD-Score:-3.29|P-value:5.08E-4||SGD DESC:Protein involved in export of proteins from the endoplasmic reticulum; ERV15 has a paralog, ERV14, that arose from the whole genome duplication Gene:ESL2(YKR096W)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Protein of unknown function that interacts with Pex14p; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; predicted to contain a PINc domain Gene:FUI1(YBL042C)|FD-Score:3.15|P-value:8.09E-4||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:HIS6(YIL020C)|FD-Score:-3.59|P-value:1.66E-4||SGD DESC:Phosphoribosyl-5-amino-1-phosphoribosyl-4-imidazolecarboxiamide isomerase, catalyzes the fourth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:IRS4(YKR019C)|FD-Score:8.17|P-value:1.58E-16||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISN1(YOR155C)|FD-Score:4.31|P-value:8.16E-6||SGD DESC:Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase, catalyzes the breakdown of IMP to inosine, does not show similarity to known 5'-nucleotidases from other organisms Gene:JEM1(YJL073W)|FD-Score:3.96|P-value:3.82E-5||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:KIN82(YCR091W)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Putative serine/threonine protein kinase implicated in the regulation of phospholipid asymmetry through the activation of phospholipid translocases (flippases) Lem3p-Dnf1p/Dnf2p; similar to Fpk1p Gene:MFA1(YDR461W)|FD-Score:-3.38|P-value:3.67E-4||SGD DESC:Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA2 Gene:MSC1(YML128C)|FD-Score:-4.29|P-value:9.12E-6||SGD DESC:Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated Gene:MTO1(YGL236C)|FD-Score:4.36|P-value:6.49E-6||SGD DESC:Mitochondrial protein, forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants Gene:PAN3(YKL025C)|FD-Score:8.31|P-value:4.79E-17||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PET309(YLR067C)|FD-Score:4.48|P-value:3.81E-6||SGD DESC:Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains seven pentatricopeptide repeats (PPRs) Gene:PFA5(YDR459C)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Palmitoyltransferase with autoacylation activity; likely functions in pathway(s) outside Ras; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain Gene:PHO90(YJL198W)|FD-Score:4.67|P-value:1.54E-6||SGD DESC:Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication Gene:PIF1(YML061C)|FD-Score:-3.77|P-value:8.23E-5||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:PKH2(YOL100W)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p Gene:PMR1(YGL167C)|FD-Score:3.93|P-value:4.32E-5||SGD DESC:High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease Gene:PMT5(YDL093W)|FD-Score:3.77|P-value:8.30E-5||SGD DESC:Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt3p, can instead interact with Pmt2p in some conditions; target for new antifungals Gene:PSY2(YNL201C)|FD-Score:-10.1|P-value:2.25E-24||SGD DESC:Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 Gene:QDR1(YIL120W)|FD-Score:-4.65|P-value:1.62E-6||SGD DESC:Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for resistance to quinidine, ketoconazole, fluconazole, and barban; QDR1 has a paralog, AQR1, that arose from the whole genome duplication Gene:RAD33(YML011C)|FD-Score:4.93|P-value:4.13E-7||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:RAD51(YER095W)|FD-Score:-4.78|P-value:8.68E-7||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RCN2(YOR220W_p)|FD-Score:5.08|P-value:1.89E-7||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; phosphorylated in response to alpha factor; protein abundance increases in response to DNA replication stress Gene:RHO5(YNL180C)|FD-Score:3.84|P-value:6.18E-5||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely involved in protein kinase C (Pkc1p)-dependent signal transduction pathway that controls cell integrity Gene:RKM1(YPL208W)|FD-Score:4.31|P-value:8.21E-6||SGD DESC:SET-domain lysine-N-methyltransferase, catalyzes the formation of dimethyllysine residues on the large ribsomal subunit proteins L23a (Rpl23Ap and Rpl23Bp) and L18 (Rps18Ap and Rps18Bp) Gene:RMA1(YKL132C)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Putative dihydrofolate synthetase; has similarity to Fol3p and to E. coli folylpolyglutamate synthetase/dihydrofolate synthetase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RPL13A(YDL082W)|FD-Score:-4.25|P-value:1.08E-5||SGD DESC:Ribosomal 60S subunit protein L13A; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13A has a paralog, RPL13B, that arose from the whole genome duplication Gene:RPL6A(YML073C)|FD-Score:-4.14|P-value:1.74E-5||SGD DESC:Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication Gene:RPS21B(YJL136C)|FD-Score:-4.15|P-value:1.69E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication Gene:RSM27(YGR215W)|FD-Score:4.48|P-value:3.82E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RTT102(YGR275W)|FD-Score:3.73|P-value:9.50E-5||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; suggested role in chromosome maintenance; possible weak regulator of Ty1 transposition; protein abundance increases in response to DNA replication stress Gene:SDS3(YIL084C)|FD-Score:3.2|P-value:6.96E-4||SGD DESC:Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes Gene:SED4(YCR067C)|FD-Score:-3.64|P-value:1.35E-4||SGD DESC:Integral endoplasmic reticulum membrane protein that stimulates Sar1p GTPase activity; involved in COPII vesicle budding through disassociation of coat proteins from membranes onto liposomes; binds Sec16p; similar to Sec12p Gene:SGF73(YGL066W)|FD-Score:3.94|P-value:4.06E-5||SGD DESC:SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay Gene:SNQ2(YDR011W)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SNZ2(YNL333W)|FD-Score:4.65|P-value:1.68E-6||SGD DESC:Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p Gene:SSD1(YDR293C)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSN2(YDR443C)|FD-Score:-3.37|P-value:3.81E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:SVF1(YDR346C)|FD-Score:3.23|P-value:6.13E-4||SGD DESC:Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance Gene:TAL1(YLR354C)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate Gene:TAT2(YOL020W)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TCB2(YNL087W)|FD-Score:4.51|P-value:3.28E-6||SGD DESC:ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains 3 calcium and lipid binding domains; mRNA is targeted to the bud Gene:TIF1(YKR059W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF1 has a paralog, TIF2, that arose from the whole genome duplication Gene:TIR3(YIL011W)|FD-Score:-4.26|P-value:1.01E-5||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth Gene:TLG2(YOL018C)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TOK1(YJL093C)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Outward-rectifier potassium channel of the plasma membrane with two pore domains in tandem, each of which forms a functional channel permeable to potassium; carboxy tail functions to prevent inner gate closures; target of K1 toxin Gene:TPO1(YLL028W)|FD-Score:-4.17|P-value:1.54E-5||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane Gene:TRM3(YDL112W)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs Gene:TRS85(YDR108W)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:TVP15(YDR100W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p Gene:UBC5(YDR059C)|FD-Score:4.05|P-value:2.55E-5||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication Gene:UBP6(YFR010W)|FD-Score:4.43|P-value:4.81E-6||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:URA1(YKL216W)|FD-Score:3.74|P-value:9.37E-5||SGD DESC:Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid Gene:VID24(YBR105C)|FD-Score:5.24|P-value:8.16E-8||SGD DESC:GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles Gene:VPS8(YAL002W)|FD-Score:-4.26|P-value:1.03E-5||SGD DESC:Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif Gene:YBR174C(YBR174C_d)|FD-Score:3.85|P-value:5.89E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YBR182C-A(YBR182C-A_p)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR196C-A(YBR196C-A_p)|FD-Score:5.68|P-value:6.57E-9||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR008C(YDR008C_d)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR115W(YDR115W_p)|FD-Score:3.73|P-value:9.39E-5||SGD DESC:Putative mitochondrial ribosomal protein of the large subunit; similar to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins; protein increases in abundance and relocalizes to the plasma membrane upon DNA replication stress Gene:YDR199W(YDR199W_d)|FD-Score:4.81|P-value:7.73E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity Gene:YER076C(YER076C_p)|FD-Score:3.81|P-value:7.04E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization Gene:YGL108C(YGL108C_p)|FD-Score:-4.96|P-value:3.61E-7||SGD DESC:Protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress Gene:YGL242C(YGL242C_p)|FD-Score:4.35|P-value:6.82E-6||SGD DESC:Putative protein of unknown function; deletion mutant is viable Gene:YJR037W(YJR037W_d)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene HUL4/YJR036C; deletion mutant has decreased spore survival in Drosophila feces Gene:YJR128W(YJR128W_d)|FD-Score:4.36|P-value:6.53E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RSF2 Gene:YLL007C(YLL007C_p)|FD-Score:4.61|P-value:2.02E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YLR253W(YLR253W_p)|FD-Score:3.33|P-value:4.38E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; transcription is periodic during the metabolic cycle; YLR253W is not an essential gene Gene:YLR294C(YLR294C_d)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ATP14 Gene:YML122C(YML122C_d)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL108C(YNL108C_p)|FD-Score:9.75|P-value:9.38E-23||SGD DESC:Putative protein of unknown function; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication Gene:YOL024W(YOL024W_p)|FD-Score:3.99|P-value:3.30E-5||SGD DESC:Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site; YOL024W has a paralog, IGD1, that arose from the whole genome duplication Gene:YPL014W(YPL014W_p)|FD-Score:3.2|P-value:6.80E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YPL107W(YPL107W_p)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YPL107W is not an essential gene Gene:YSC84(YHR016C)|FD-Score:6.21|P-value:2.61E-10||SGD DESC:Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR037W7.601.47E-141.66KRS1Lysyl-tRNA synthetase
YBR142W5.941.43E-90.91MAK5Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits
YJR023C_d5.032.46E-70.19YJR023C_dPutative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing
YBR265W4.846.58E-70.28TSC103-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family
YDR361C4.562.57E-60.03BCP1Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport
YDR081C4.532.95E-60.46PDC2Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions
YBR243C4.072.31E-50.18ALG7UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin
YIL104C3.904.91E-50.05SHQ1Chaperone protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones
YFL037W3.846.09E-50.26TUB2Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria
YGR253C3.581.73E-40.10PUP2Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta
YGL097W3.482.48E-40.05SRM1Nucleotide exchange factor for Gsp1p, localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p
YOR168W3.432.96E-40.08GLN4Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions
YDR240C3.363.91E-40.03SNU56Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex
YPL235W3.334.35E-40.05RVB2ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly
YNL152W3.285.24E-40.00INN1Essential protein that associates with contractile actomyosin ring; required for ingression of the plasma membrane into the bud neck during cytokinesis; C2 domain, a membrane targeting module, is required for function; activates chitin synthase activity of Chs2p during cytokinesis

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNL108C_p9.759.38E-23YNL108C_pPutative protein of unknown function; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication
YKL025C8.314.79E-17PAN3Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes
YKR019C8.171.58E-16IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YHR016C6.212.61E-10YSC84Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication
YBR196C-A_p5.686.57E-9YBR196C-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YPL051W5.305.71E-8ARL3GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor
YBR105C5.248.16E-8VID24GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles
YDR197W5.238.34E-8CBS2Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader
YOR220W_p5.081.89E-7RCN2_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; phosphorylated in response to alpha factor; protein abundance increases in response to DNA replication stress
YNR007C5.052.16E-7ATG3E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p
YKL103C4.934.01E-7APE1Vacuolar aminopeptidase yscI; zinc metalloproteinase that belongs to the peptidase family M18; often used as a marker protein in studies of autophagy and cytosol to vacuole targeting (CVT) pathway; protein increases in abundance and relative distribution to cytoplasmic foci increases upon DNA replication stress
YML011C4.934.13E-7RAD33Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus
YDR199W_d4.817.73E-7YDR199W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity
YLR418C4.671.47E-6CDC73Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress
YJL198W4.671.54E-6PHO90Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication

GO enrichment analysis for SGTC_2942
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1314.36E-24SGTC_29459067681 61.8 μMChembridge (Drug-like library)164569300.15942
0.1095.53E-17SGTC_10603448-7350 82.0 μMChemDiv (Drug-like library)5701780.185185Golgi
0.0992.05E-14SGTC_910417-1751 58.4 μMChemDiv (Drug-like library)67956240.08Golgi
0.0984.68E-14SGTC_29439068529 45.3 μMYeast Active Library32706340.333333
0.0921.31E-12SGTC_980074-0023 107.7 μMChemDiv (Drug-like library)35638210.1Golgi
0.0913.21E-12SGTC_6320781-2353 4.7 μMChemDiv (Drug-like library)68115150.108108Golgi
0.0897.19E-12SGTC_560957-0365 83.2 μMChemDiv (Drug-like library)67523600.0609756Golgi
0.0863.89E-11SGTC_9701315-0089 19.5 μMChemDiv (Drug-like library)68110830.272727RSC & ERG11
0.0822.72E-10SGTC_28729044037 26.0 μMChembridge (Drug-like library)64651950.710526
0.0808.10E-10SGTC_4982,5-di-tert-butyl-1,4-hydroquinone (DTBHQ) 150.0 μMICCB bioactive library23740.0652174Golgi
0.0773.87E-9SGTC_7830848-0141 92.7 μMChemDiv (Drug-like library)67550760.0416667RSC & ERG11
0.0766.05E-9SGTC_2587piperic acid 92.2 μMMicrosource (Natural product library)53705360.116667
0.0757.32E-9SGTC_15324',5'-dihydroxyflavone 78.7 μMTimTec (Pure natural product library)1655210.1Golgi
0.0758.06E-9SGTC_1034k072-0165 12.7 μMChemDiv (Drug-like library)58354610.112903Golgi
0.0741.21E-8SGTC_820054-0107 293.8 μMChemDiv (Drug-like library)31046970.09375

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2872904403725.97 μM0.7105266465195Chembridge (Drug-like library)284.1131234.14912
SGTC_1190121-004324.82 μM0.5106381092378ChemDiv (Drug-like library)405.469124.53322mitochondrial stress
SGTC_2889905965651.95 μM0.53949838Chembridge (Drug-like library)287.1848563.64216
SGTC_3278913960549.47 μM0.4888897958859Chembridge (Drug-like library)267.6585263.6913
SGTC_2890904672958.44 μM0.479167903329Chembridge (Drug-like library)270.114662.88412Golgi
SGTC_2392N-(3-chlorophenyl)-6-oxo-1H-pyridine-3-carboxamide200 μM0.458333803109Chembridge (Fragment library)248.665061.08522
SGTC_2787528454071.43 μM0.458333736958Chembridge (Drug-like library)291.729563.24613
SGTC_3176910658249.47 μM0.4417018027Chembridge (Drug-like library)275.730163.74812
SGTC_2900532852885.29 μM0.4375795359Chembridge (Drug-like library)261.703583.26212Golgi
SGTC_3173910569349.47 μM0.42307717096658Chembridge (Drug-like library)289.756744.13512