9068529

5-bromo-N-(3-chloro-4-fluorophenyl)pyridine-3-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2943
Screen concentration 45.3 μM
Source Yeast Active Library
PubChem CID 3270634
SMILES C1=CC(=C(C=C1NC(=O)C2=CC(=CN=C2)Br)Cl)F
Standardized SMILES Fc1ccc(NC(=O)c2cncc(Br)c2)cc1Cl
Molecular weight 329.5522
ALogP 3.08
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 25.18
% growth inhibition (Hom. pool) 12.58


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3270634
Download HIP data (tab-delimited text)  (excel)
Gene:ACS2(YLR153C)|FD-Score:3.47|P-value:2.60E-4|Clearance:0.07||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions Gene:BRL1(YHR036W)|FD-Score:-3.52|P-value:2.18E-4|Clearance:0||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:CCA1(YER168C)|FD-Score:4.66|P-value:1.58E-6|Clearance:0.06||SGD DESC:ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites Gene:CDC48(YDL126C)|FD-Score:-4.14|P-value:1.76E-5|Clearance:0||SGD DESC:AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP Gene:CSE1(YGL238W)|FD-Score:3.76|P-value:8.35E-5|Clearance:0.13||SGD DESC:Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation Gene:CYR1(YJL005W)|FD-Score:-4.43|P-value:4.64E-6|Clearance:0||SGD DESC:Adenylate cyclase, required for cAMP production and cAMP-dependent protein kinase signaling; the cAMP pathway controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation Gene:DPS1(YLL018C)|FD-Score:-3.44|P-value:2.89E-4|Clearance:0||SGD DESC:Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation Gene:ERO1(YML130C)|FD-Score:-3.21|P-value:6.62E-4|Clearance:0||SGD DESC:Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds Gene:FRQ1(YDR373W)|FD-Score:4.87|P-value:5.50E-7|Clearance:0.21||SGD DESC:N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily Gene:GCD6(YDR211W)|FD-Score:-3.84|P-value:6.10E-5|Clearance:0||SGD DESC:Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress Gene:IRR1(YIL026C)|FD-Score:4.98|P-value:3.10E-7|Clearance:0.11||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:LCP5(YER127W)|FD-Score:5.16|P-value:1.25E-7|Clearance:0.08||SGD DESC:Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus Gene:NMD3(YHR170W)|FD-Score:3.36|P-value:3.86E-4|Clearance:0.09||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOP8(YOL144W)|FD-Score:7.66|P-value:9.36E-15|Clearance:1.65||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:POL5(YEL055C)|FD-Score:3.64|P-value:1.38E-4|Clearance:0.14||SGD DESC:DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA Gene:PRP3(YDR473C)|FD-Score:4.6|P-value:2.15E-6|Clearance:0.83||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RAD53(YPL153C)|FD-Score:5.08|P-value:1.86E-7|Clearance:0.1||SGD DESC:Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication Gene:RIM2(YBR192W)|FD-Score:8.47|P-value:1.23E-17|Clearance:1.65||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RPB10(YOR210W)|FD-Score:-4.89|P-value:5.05E-7|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPL18A(YOL120C)|FD-Score:3.5|P-value:2.37E-4|Clearance:0.03||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RRN6(YBL014C)|FD-Score:-3.34|P-value:4.19E-4|Clearance:0||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:SEC26(YDR238C)|FD-Score:6.01|P-value:9.31E-10|Clearance:0.31||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SRP102(YKL154W)|FD-Score:3.16|P-value:7.87E-4|Clearance:0.13||SGD DESC:Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane Gene:TFB2(YPL122C)|FD-Score:5.7|P-value:5.88E-9|Clearance:0.08||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH Gene:TUB2(YFL037W)|FD-Score:3.4|P-value:3.40E-4|Clearance:0.03||SGD DESC:Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria Gene:YBR190W(YBR190W_d)|FD-Score:3.27|P-value:5.42E-4|Clearance:0.11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YIF1(YNL263C)|FD-Score:-3.33|P-value:4.31E-4|Clearance:0||SGD DESC:Integral membrane protein required for the fusion of ER-derived COPII transport vesicles with the Golgi; interacts with Yip1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF3 Gene:YMR290W-A(YMR290W-A_d)|FD-Score:5.63|P-value:9.14E-9|Clearance:0.47||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:YNL114C(YNL114C_d)|FD-Score:-3.53|P-value:2.08E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Gene:YTM1(YOR272W)|FD-Score:-5.31|P-value:5.61E-8|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats Gene:ACS2(YLR153C)|FD-Score:3.47|P-value:2.60E-4|Clearance:0.07||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions Gene:BRL1(YHR036W)|FD-Score:-3.52|P-value:2.18E-4|Clearance:0||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:CCA1(YER168C)|FD-Score:4.66|P-value:1.58E-6|Clearance:0.06||SGD DESC:ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites Gene:CDC48(YDL126C)|FD-Score:-4.14|P-value:1.76E-5|Clearance:0||SGD DESC:AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP Gene:CSE1(YGL238W)|FD-Score:3.76|P-value:8.35E-5|Clearance:0.13||SGD DESC:Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation Gene:CYR1(YJL005W)|FD-Score:-4.43|P-value:4.64E-6|Clearance:0||SGD DESC:Adenylate cyclase, required for cAMP production and cAMP-dependent protein kinase signaling; the cAMP pathway controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation Gene:DPS1(YLL018C)|FD-Score:-3.44|P-value:2.89E-4|Clearance:0||SGD DESC:Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation Gene:ERO1(YML130C)|FD-Score:-3.21|P-value:6.62E-4|Clearance:0||SGD DESC:Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds Gene:FRQ1(YDR373W)|FD-Score:4.87|P-value:5.50E-7|Clearance:0.21||SGD DESC:N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily Gene:GCD6(YDR211W)|FD-Score:-3.84|P-value:6.10E-5|Clearance:0||SGD DESC:Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress Gene:IRR1(YIL026C)|FD-Score:4.98|P-value:3.10E-7|Clearance:0.11||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:LCP5(YER127W)|FD-Score:5.16|P-value:1.25E-7|Clearance:0.08||SGD DESC:Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus Gene:NMD3(YHR170W)|FD-Score:3.36|P-value:3.86E-4|Clearance:0.09||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOP8(YOL144W)|FD-Score:7.66|P-value:9.36E-15|Clearance:1.65||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:POL5(YEL055C)|FD-Score:3.64|P-value:1.38E-4|Clearance:0.14||SGD DESC:DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA Gene:PRP3(YDR473C)|FD-Score:4.6|P-value:2.15E-6|Clearance:0.83||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RAD53(YPL153C)|FD-Score:5.08|P-value:1.86E-7|Clearance:0.1||SGD DESC:Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication Gene:RIM2(YBR192W)|FD-Score:8.47|P-value:1.23E-17|Clearance:1.65||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RPB10(YOR210W)|FD-Score:-4.89|P-value:5.05E-7|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPL18A(YOL120C)|FD-Score:3.5|P-value:2.37E-4|Clearance:0.03||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RRN6(YBL014C)|FD-Score:-3.34|P-value:4.19E-4|Clearance:0||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:SEC26(YDR238C)|FD-Score:6.01|P-value:9.31E-10|Clearance:0.31||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SRP102(YKL154W)|FD-Score:3.16|P-value:7.87E-4|Clearance:0.13||SGD DESC:Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane Gene:TFB2(YPL122C)|FD-Score:5.7|P-value:5.88E-9|Clearance:0.08||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH Gene:TUB2(YFL037W)|FD-Score:3.4|P-value:3.40E-4|Clearance:0.03||SGD DESC:Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria Gene:YBR190W(YBR190W_d)|FD-Score:3.27|P-value:5.42E-4|Clearance:0.11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YIF1(YNL263C)|FD-Score:-3.33|P-value:4.31E-4|Clearance:0||SGD DESC:Integral membrane protein required for the fusion of ER-derived COPII transport vesicles with the Golgi; interacts with Yip1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF3 Gene:YMR290W-A(YMR290W-A_d)|FD-Score:5.63|P-value:9.14E-9|Clearance:0.47||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:YNL114C(YNL114C_d)|FD-Score:-3.53|P-value:2.08E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Gene:YTM1(YOR272W)|FD-Score:-5.31|P-value:5.61E-8|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3270634
Download HOP data (tab-delimited text)  (excel)
Gene:AEP2(YMR282C)|FD-Score:3.25|P-value:5.84E-4||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AFT1(YGL071W)|FD-Score:3.14|P-value:8.40E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ALG12(YNR030W)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation Gene:ALT2(YDR111C_p)|FD-Score:-3.85|P-value:5.88E-5||SGD DESC:Catalytically inactive paralog of ALT1, an alanine transaminase; expression is repressed in the presence of alanine and repression is mediated by Nrg1p Gene:ARK1(YNL020C)|FD-Score:-3.68|P-value:1.16E-4||SGD DESC:Serine/threonine protein kinase; involved in regulation of the cortical actin cytoskeleton; involved in control of endocytosis; ARK1 has a paralog, PRK1, that arose from the whole genome duplication Gene:ARL3(YPL051W)|FD-Score:5.89|P-value:1.98E-9||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ARO1(YDR127W)|FD-Score:-3.24|P-value:6.00E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO80(YDR421W)|FD-Score:-3.22|P-value:6.33E-4||SGD DESC:Zinc finger transcriptional activator of the Zn2Cys6 family; activates transcription of aromatic amino acid catabolic genes in the presence of aromatic amino acids Gene:ATG20(YDL113C)|FD-Score:-3.52|P-value:2.14E-4||SGD DESC:Sorting nexin family member required for the cytoplasm-to-vacuole targeting (Cvt) pathway and for endosomal sorting; has a Phox homology domain that binds phosphatidylinositol-3-phosphate; interacts with Snx4p; potential Cdc28p substrate Gene:BIT2(YBR270C_p)|FD-Score:3.8|P-value:7.36E-5||SGD DESC:Subunit of TORC2 membrane-associated complex; involved in regulation of actin cytoskeletal dynamics during polarized growth and cell wall integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; BIT2 has a paralog, BIT61, that arose from the whole genome duplication Gene:CBS2(YDR197W)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader Gene:CGI121(YML036W)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Component of the EKC/KEOPS complex with Bud32p, Kae1p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription; Cgi121p is dispensable for tRNA modification Gene:CMR3(YPR013C_p)|FD-Score:-3.33|P-value:4.34E-4||SGD DESC:Putative zinc finger protein; YPR013C is not an essential gene Gene:COG8(YML071C)|FD-Score:4.13|P-value:1.81E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ3(YOL096C)|FD-Score:-3.44|P-value:2.89E-4||SGD DESC:O-methyltransferase, catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein Gene:CPR5(YDR304C)|FD-Score:-7.14|P-value:4.52E-13||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER Gene:CRG1(YHR209W)|FD-Score:-3.71|P-value:1.04E-4||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:CUE1(YMR264W)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:CYT2(YKL087C)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1 Gene:DAL81(YIR023W)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DEG1(YFL001W)|FD-Score:4.13|P-value:1.80E-5||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability Gene:ECM3(YOR092W)|FD-Score:3.11|P-value:9.39E-4||SGD DESC:Non-essential protein of unknown function; involved in signal transduction and the genotoxic response; induced rapidly in response to treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from ER to cytoplasm upon DNA replication stress; ECM3 has a paralog, YNL095C, that arose from the whole genome duplication Gene:EFT2(YDR385W)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT2 has a paralog, EFT1, that arose from the whole genome duplication Gene:ELG1(YOR144C)|FD-Score:3.72|P-value:9.99E-5||SGD DESC:Subunit of an alternative replication factor C complex important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication Gene:FKS3(YMR306W)|FD-Score:5.58|P-value:1.20E-8||SGD DESC:Protein involved in spore wall assembly, has similarity to 1,3-beta-D-glucan synthase catalytic subunits Fks1p and Gsc2p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRE7(YOL152W)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels Gene:GLC8(YMR311C)|FD-Score:5.26|P-value:7.29E-8||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:HBN1(YCL026C-B_p)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:HUA1(YGR268C)|FD-Score:-3.76|P-value:8.61E-5||SGD DESC:Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:HXK1(YFR053C)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication Gene:ICP55(YER078C)|FD-Score:4.45|P-value:4.37E-6||SGD DESC:Mitochondrial aminopeptidase; cleaves the N termini of at least 38 imported proteins after cleavage by the mitochondrial processing peptidase (MPP), thereby increasing their stability; member of the aminopeptidase P family Gene:ILM1(YJR118C)|FD-Score:-4.16|P-value:1.60E-5||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:ISC10(YER180C)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:ISN1(YOR155C)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase, catalyzes the breakdown of IMP to inosine, does not show similarity to known 5'-nucleotidases from other organisms Gene:LAA1(YJL207C)|FD-Score:-4.2|P-value:1.36E-5||SGD DESC:AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene Gene:LEM3(YNL323W)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LIF1(YGL090W)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Component of the DNA ligase IV complex that mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein Gene:LST4(YKL176C)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:LYS4(YDR234W)|FD-Score:4.09|P-value:2.17E-5||SGD DESC:Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway Gene:MCH4(YOL119C)|FD-Score:3.77|P-value:8.02E-5||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MDJ1(YFL016C)|FD-Score:6.32|P-value:1.30E-10||SGD DESC:Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones Gene:MRPL31(YKL138C)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRT4(YKL009W)|FD-Score:-4.98|P-value:3.12E-7||SGD DESC:Protein involved in mRNA turnover and ribosome assembly, localizes to the nucleolus Gene:MSC7(YHR039C)|FD-Score:3.15|P-value:8.15E-4||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids Gene:MUM2(YBR057C)|FD-Score:4.43|P-value:4.61E-6||SGD DESC:Cytoplasmic protein essential for meiotic DNA replication and sporulation; interacts with Orc2p, which is a component of the origin recognition complex Gene:NAM2(YLR382C)|FD-Score:3.43|P-value:3.07E-4||SGD DESC:Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Gene:NBP2(YDR162C)|FD-Score:-3.77|P-value:8.27E-5||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NIS1(YNL078W)|FD-Score:-3.46|P-value:2.70E-4||SGD DESC:Protein localized in the bud neck at G2/M phase; physically interacts with septins; possibly involved in a mitotic signaling network Gene:OCA1(YNL099C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:ORM1(YGR038W)|FD-Score:5.11|P-value:1.57E-7||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:PAD1(YDR538W)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Phenylacrylic acid decarboxylase, confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX Gene:PCL8(YPL219W)|FD-Score:5.25|P-value:7.43E-8||SGD DESC:Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation Gene:PET122(YER153C)|FD-Score:3.1|P-value:9.60E-4||SGD DESC:Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet494p; located in the mitochondrial inner membrane Gene:PHR1(YOR386W)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:DNA photolyase involved in photoreactivation, repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p Gene:PMP1(YCR024C-A)|FD-Score:-4|P-value:3.19E-5||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:PPE1(YHR075C)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Protein with carboxyl methyl esterase activity that may have a role in demethylation of the phosphoprotein phosphatase catalytic subunit; also identified as a small subunit mitochondrial ribosomal protein Gene:PSY2(YNL201C)|FD-Score:-5.67|P-value:6.97E-9||SGD DESC:Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 Gene:QCR10(YHR001W-A)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:Subunit of the ubiqunol-cytochrome c oxidoreductase complex which includes Cobp, Rip1p, Cyt1p, Cor1p, Qcr2p, Qcr6p, Qcr7p, Qcr8p, Qcr9p, and Qcr10p and comprises part of the mitochondrial respiratory chain Gene:RCK1(YGL158W)|FD-Score:-3.74|P-value:9.08E-5||SGD DESC:Protein kinase involved in the response to oxidative stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; RCK1 has a paralog, RCK2, that arose from the whole genome duplication Gene:RED1(YLR263W)|FD-Score:4.36|P-value:6.64E-6||SGD DESC:Protein component of the synaptonemal complex axial elements, involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; interacts with Hop1p, Mec3p and Ddc1p Gene:RIM13(YMR154C)|FD-Score:-3.56|P-value:1.83E-4||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RNH202(YDR279W)|FD-Score:3.36|P-value:3.87E-4||SGD DESC:Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS2 that causes Aicardi-Goutieres syndrome Gene:SAC6(YDR129C)|FD-Score:-4.69|P-value:1.35E-6||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SIL1(YOL031C)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein Gene:SIT4(YDL047W)|FD-Score:-3.26|P-value:5.48E-4||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:SLM3(YDL033C)|FD-Score:3.84|P-value:6.08E-5||SGD DESC:tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) Gene:SLM6(YBR266C_d)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase Gene:SPS19(YNL202W)|FD-Score:5.59|P-value:1.13E-8||SGD DESC:Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate Gene:SPT3(YDR392W)|FD-Score:-6.45|P-value:5.46E-11||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:STB2(YMR053C)|FD-Score:-3.89|P-value:5.03E-5||SGD DESC:Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p Gene:SWC3(YAL011W)|FD-Score:-3.53|P-value:2.10E-4||SGD DESC:Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae Gene:TEP1(YNL128W)|FD-Score:-5.83|P-value:2.69E-9||SGD DESC:PTEN homolog with no demonstrated inositol lipid phosphatase activity; plays a role in normal sporulation; homolog of human tumor suppressor gene PTEN/MMAC1/TEP1 and fission yeast ptn1 Gene:TRE1(YPL176C)|FD-Score:-3.41|P-value:3.24E-4||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:UBC12(YLR306W)|FD-Score:-4.09|P-value:2.18E-5||SGD DESC:Enzyme that mediates the conjugation of Rub1p, a ubiquitin-like protein, to other proteins; related to E2 ubiquitin-conjugating enzymes Gene:UIP4(YPL186C)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Protein that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates; detected in a phosphorylated state in the mitochondrial outer membrane; also detected in ER and nuclear envelope Gene:UME6(YDR207C)|FD-Score:3.18|P-value:7.29E-4||SGD DESC:Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p Gene:UPS1(YLR193C)|FD-Score:-3.11|P-value:9.34E-4||SGD DESC:Phosphatidic acid transfer protein; plays a role in phospholipid metabolism by transporting phosphatidic acid from the outer to the inner mitochondrial membrane; localizes to the mitochondrial intermembrane space; null mutant has altered cardiolipin and phosphatidic acid levels; ortholog of human PRELI Gene:URA4(YLR420W)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate Gene:VMA1(YDL185W)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VPH1(YOR270C)|FD-Score:-3.28|P-value:5.28E-4||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:WTM1(YOR230W)|FD-Score:-3.25|P-value:5.87E-4||SGD DESC:Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; required for nuclear localization of the ribonucleotide reductase small subunit Rnr2p and Rnr4p; contains WD repeats Gene:YCK3(YER123W)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YCR075W-A(YCR075W-A_p)|FD-Score:-3.97|P-value:3.67E-5||SGD DESC:Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication Gene:YCR085W(YCR085W_d)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR194W-A(YDR194W-A_p)|FD-Score:3.17|P-value:7.69E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YER130C(YER130C_p)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YGL176C(YGL176C_p)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Putative protein of unknown function; deletion mutant is viable and has no detectable phenotype Gene:YGR079W(YGR079W_p)|FD-Score:-3.32|P-value:4.45E-4||SGD DESC:Putative protein of unknown function; YGR079W is not an essential gene Gene:YHK8(YHR048W_p)|FD-Score:3.16|P-value:8.01E-4||SGD DESC:Presumed antiporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; expression of gene is up-regulated in cells exhibiting reduced susceptibility to azoles Gene:YHR049C-A(YHR049C-A_d)|FD-Score:-3.42|P-value:3.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR005C-A(YJR005C-A_p)|FD-Score:-3.11|P-value:9.25E-4||SGD DESC:Putative protein of unknown function; originally identified as a syntenic homolog of an <i>Ashbya gossypii</i> gene; YJR005C-A has a paralog, YGR169C-A, that arose from the whole genome duplication Gene:YJR151W-A(YJR151W-A_p)|FD-Score:-3.86|P-value:5.68E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR; predicted to have a role in transcription based on computational guilt by association analysis Gene:YKL069W(YKL069W)|FD-Score:-3.21|P-value:6.56E-4||SGD DESC:Methionine-R-sulfoxide reductase; reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress; relative distribution to the nucleus increases upon DNA replication stress Gene:YKR075C(YKR075C_p)|FD-Score:-4.18|P-value:1.45E-5||SGD DESC:Protein of unknown function; similar to YOR062Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YLR241W(YLR241W_p)|FD-Score:-3.18|P-value:7.38E-4||SGD DESC:Putative protein of unknown function, may be involved in detoxification Gene:YLR294C(YLR294C_d)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ATP14 Gene:YLR358C(YLR358C_p)|FD-Score:4.33|P-value:7.61E-6||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps ORF RSC2/YLR357W Gene:YMR247W-A(YMR247W-A_p)|FD-Score:-3.75|P-value:8.72E-5||SGD DESC:Putative protein of unknown function Gene:YMR279C(YMR279C)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Putative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; YMR279C has a paralog, ATR1, that arose from the whole genome duplication Gene:YNL120C(YNL120C_d)|FD-Score:3.33|P-value:4.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion enhances replication of Brome mosaic virus in S. cerevisiae, but likely due to effects on the overlapping gene Gene:YNR066C(YNR066C_p)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:Putative membrane-localized protein of unknown function Gene:YOL037C(YOL037C_d)|FD-Score:-3.24|P-value:5.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOL036W Gene:YPL257W(YPL257W_p)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene Gene:YPL261C(YPL261C_d)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YPL260W Gene:YPR096C(YPR096C)|FD-Score:4.22|P-value:1.25E-5||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:AEP2(YMR282C)|FD-Score:3.25|P-value:5.84E-4||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AFT1(YGL071W)|FD-Score:3.14|P-value:8.40E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ALG12(YNR030W)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation Gene:ALT2(YDR111C_p)|FD-Score:-3.85|P-value:5.88E-5||SGD DESC:Catalytically inactive paralog of ALT1, an alanine transaminase; expression is repressed in the presence of alanine and repression is mediated by Nrg1p Gene:ARK1(YNL020C)|FD-Score:-3.68|P-value:1.16E-4||SGD DESC:Serine/threonine protein kinase; involved in regulation of the cortical actin cytoskeleton; involved in control of endocytosis; ARK1 has a paralog, PRK1, that arose from the whole genome duplication Gene:ARL3(YPL051W)|FD-Score:5.89|P-value:1.98E-9||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ARO1(YDR127W)|FD-Score:-3.24|P-value:6.00E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO80(YDR421W)|FD-Score:-3.22|P-value:6.33E-4||SGD DESC:Zinc finger transcriptional activator of the Zn2Cys6 family; activates transcription of aromatic amino acid catabolic genes in the presence of aromatic amino acids Gene:ATG20(YDL113C)|FD-Score:-3.52|P-value:2.14E-4||SGD DESC:Sorting nexin family member required for the cytoplasm-to-vacuole targeting (Cvt) pathway and for endosomal sorting; has a Phox homology domain that binds phosphatidylinositol-3-phosphate; interacts with Snx4p; potential Cdc28p substrate Gene:BIT2(YBR270C_p)|FD-Score:3.8|P-value:7.36E-5||SGD DESC:Subunit of TORC2 membrane-associated complex; involved in regulation of actin cytoskeletal dynamics during polarized growth and cell wall integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; BIT2 has a paralog, BIT61, that arose from the whole genome duplication Gene:CBS2(YDR197W)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader Gene:CGI121(YML036W)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Component of the EKC/KEOPS complex with Bud32p, Kae1p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription; Cgi121p is dispensable for tRNA modification Gene:CMR3(YPR013C_p)|FD-Score:-3.33|P-value:4.34E-4||SGD DESC:Putative zinc finger protein; YPR013C is not an essential gene Gene:COG8(YML071C)|FD-Score:4.13|P-value:1.81E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ3(YOL096C)|FD-Score:-3.44|P-value:2.89E-4||SGD DESC:O-methyltransferase, catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein Gene:CPR5(YDR304C)|FD-Score:-7.14|P-value:4.52E-13||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER Gene:CRG1(YHR209W)|FD-Score:-3.71|P-value:1.04E-4||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:CUE1(YMR264W)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:CYT2(YKL087C)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1 Gene:DAL81(YIR023W)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DEG1(YFL001W)|FD-Score:4.13|P-value:1.80E-5||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability Gene:ECM3(YOR092W)|FD-Score:3.11|P-value:9.39E-4||SGD DESC:Non-essential protein of unknown function; involved in signal transduction and the genotoxic response; induced rapidly in response to treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from ER to cytoplasm upon DNA replication stress; ECM3 has a paralog, YNL095C, that arose from the whole genome duplication Gene:EFT2(YDR385W)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT2 has a paralog, EFT1, that arose from the whole genome duplication Gene:ELG1(YOR144C)|FD-Score:3.72|P-value:9.99E-5||SGD DESC:Subunit of an alternative replication factor C complex important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication Gene:FKS3(YMR306W)|FD-Score:5.58|P-value:1.20E-8||SGD DESC:Protein involved in spore wall assembly, has similarity to 1,3-beta-D-glucan synthase catalytic subunits Fks1p and Gsc2p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRE7(YOL152W)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels Gene:GLC8(YMR311C)|FD-Score:5.26|P-value:7.29E-8||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:HBN1(YCL026C-B_p)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:HUA1(YGR268C)|FD-Score:-3.76|P-value:8.61E-5||SGD DESC:Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:HXK1(YFR053C)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication Gene:ICP55(YER078C)|FD-Score:4.45|P-value:4.37E-6||SGD DESC:Mitochondrial aminopeptidase; cleaves the N termini of at least 38 imported proteins after cleavage by the mitochondrial processing peptidase (MPP), thereby increasing their stability; member of the aminopeptidase P family Gene:ILM1(YJR118C)|FD-Score:-4.16|P-value:1.60E-5||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:ISC10(YER180C)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:ISN1(YOR155C)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase, catalyzes the breakdown of IMP to inosine, does not show similarity to known 5'-nucleotidases from other organisms Gene:LAA1(YJL207C)|FD-Score:-4.2|P-value:1.36E-5||SGD DESC:AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene Gene:LEM3(YNL323W)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LIF1(YGL090W)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Component of the DNA ligase IV complex that mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein Gene:LST4(YKL176C)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:LYS4(YDR234W)|FD-Score:4.09|P-value:2.17E-5||SGD DESC:Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway Gene:MCH4(YOL119C)|FD-Score:3.77|P-value:8.02E-5||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MDJ1(YFL016C)|FD-Score:6.32|P-value:1.30E-10||SGD DESC:Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones Gene:MRPL31(YKL138C)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRT4(YKL009W)|FD-Score:-4.98|P-value:3.12E-7||SGD DESC:Protein involved in mRNA turnover and ribosome assembly, localizes to the nucleolus Gene:MSC7(YHR039C)|FD-Score:3.15|P-value:8.15E-4||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids Gene:MUM2(YBR057C)|FD-Score:4.43|P-value:4.61E-6||SGD DESC:Cytoplasmic protein essential for meiotic DNA replication and sporulation; interacts with Orc2p, which is a component of the origin recognition complex Gene:NAM2(YLR382C)|FD-Score:3.43|P-value:3.07E-4||SGD DESC:Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Gene:NBP2(YDR162C)|FD-Score:-3.77|P-value:8.27E-5||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NIS1(YNL078W)|FD-Score:-3.46|P-value:2.70E-4||SGD DESC:Protein localized in the bud neck at G2/M phase; physically interacts with septins; possibly involved in a mitotic signaling network Gene:OCA1(YNL099C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:ORM1(YGR038W)|FD-Score:5.11|P-value:1.57E-7||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:PAD1(YDR538W)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Phenylacrylic acid decarboxylase, confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX Gene:PCL8(YPL219W)|FD-Score:5.25|P-value:7.43E-8||SGD DESC:Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation Gene:PET122(YER153C)|FD-Score:3.1|P-value:9.60E-4||SGD DESC:Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet494p; located in the mitochondrial inner membrane Gene:PHR1(YOR386W)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:DNA photolyase involved in photoreactivation, repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p Gene:PMP1(YCR024C-A)|FD-Score:-4|P-value:3.19E-5||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:PPE1(YHR075C)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Protein with carboxyl methyl esterase activity that may have a role in demethylation of the phosphoprotein phosphatase catalytic subunit; also identified as a small subunit mitochondrial ribosomal protein Gene:PSY2(YNL201C)|FD-Score:-5.67|P-value:6.97E-9||SGD DESC:Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 Gene:QCR10(YHR001W-A)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:Subunit of the ubiqunol-cytochrome c oxidoreductase complex which includes Cobp, Rip1p, Cyt1p, Cor1p, Qcr2p, Qcr6p, Qcr7p, Qcr8p, Qcr9p, and Qcr10p and comprises part of the mitochondrial respiratory chain Gene:RCK1(YGL158W)|FD-Score:-3.74|P-value:9.08E-5||SGD DESC:Protein kinase involved in the response to oxidative stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; RCK1 has a paralog, RCK2, that arose from the whole genome duplication Gene:RED1(YLR263W)|FD-Score:4.36|P-value:6.64E-6||SGD DESC:Protein component of the synaptonemal complex axial elements, involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; interacts with Hop1p, Mec3p and Ddc1p Gene:RIM13(YMR154C)|FD-Score:-3.56|P-value:1.83E-4||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RNH202(YDR279W)|FD-Score:3.36|P-value:3.87E-4||SGD DESC:Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS2 that causes Aicardi-Goutieres syndrome Gene:SAC6(YDR129C)|FD-Score:-4.69|P-value:1.35E-6||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SIL1(YOL031C)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein Gene:SIT4(YDL047W)|FD-Score:-3.26|P-value:5.48E-4||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:SLM3(YDL033C)|FD-Score:3.84|P-value:6.08E-5||SGD DESC:tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) Gene:SLM6(YBR266C_d)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase Gene:SPS19(YNL202W)|FD-Score:5.59|P-value:1.13E-8||SGD DESC:Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate Gene:SPT3(YDR392W)|FD-Score:-6.45|P-value:5.46E-11||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:STB2(YMR053C)|FD-Score:-3.89|P-value:5.03E-5||SGD DESC:Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p Gene:SWC3(YAL011W)|FD-Score:-3.53|P-value:2.10E-4||SGD DESC:Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae Gene:TEP1(YNL128W)|FD-Score:-5.83|P-value:2.69E-9||SGD DESC:PTEN homolog with no demonstrated inositol lipid phosphatase activity; plays a role in normal sporulation; homolog of human tumor suppressor gene PTEN/MMAC1/TEP1 and fission yeast ptn1 Gene:TRE1(YPL176C)|FD-Score:-3.41|P-value:3.24E-4||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:UBC12(YLR306W)|FD-Score:-4.09|P-value:2.18E-5||SGD DESC:Enzyme that mediates the conjugation of Rub1p, a ubiquitin-like protein, to other proteins; related to E2 ubiquitin-conjugating enzymes Gene:UIP4(YPL186C)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Protein that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates; detected in a phosphorylated state in the mitochondrial outer membrane; also detected in ER and nuclear envelope Gene:UME6(YDR207C)|FD-Score:3.18|P-value:7.29E-4||SGD DESC:Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p Gene:UPS1(YLR193C)|FD-Score:-3.11|P-value:9.34E-4||SGD DESC:Phosphatidic acid transfer protein; plays a role in phospholipid metabolism by transporting phosphatidic acid from the outer to the inner mitochondrial membrane; localizes to the mitochondrial intermembrane space; null mutant has altered cardiolipin and phosphatidic acid levels; ortholog of human PRELI Gene:URA4(YLR420W)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate Gene:VMA1(YDL185W)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VPH1(YOR270C)|FD-Score:-3.28|P-value:5.28E-4||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:WTM1(YOR230W)|FD-Score:-3.25|P-value:5.87E-4||SGD DESC:Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; required for nuclear localization of the ribonucleotide reductase small subunit Rnr2p and Rnr4p; contains WD repeats Gene:YCK3(YER123W)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YCR075W-A(YCR075W-A_p)|FD-Score:-3.97|P-value:3.67E-5||SGD DESC:Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication Gene:YCR085W(YCR085W_d)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR194W-A(YDR194W-A_p)|FD-Score:3.17|P-value:7.69E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YER130C(YER130C_p)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YGL176C(YGL176C_p)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Putative protein of unknown function; deletion mutant is viable and has no detectable phenotype Gene:YGR079W(YGR079W_p)|FD-Score:-3.32|P-value:4.45E-4||SGD DESC:Putative protein of unknown function; YGR079W is not an essential gene Gene:YHK8(YHR048W_p)|FD-Score:3.16|P-value:8.01E-4||SGD DESC:Presumed antiporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; expression of gene is up-regulated in cells exhibiting reduced susceptibility to azoles Gene:YHR049C-A(YHR049C-A_d)|FD-Score:-3.42|P-value:3.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR005C-A(YJR005C-A_p)|FD-Score:-3.11|P-value:9.25E-4||SGD DESC:Putative protein of unknown function; originally identified as a syntenic homolog of an <i>Ashbya gossypii</i> gene; YJR005C-A has a paralog, YGR169C-A, that arose from the whole genome duplication Gene:YJR151W-A(YJR151W-A_p)|FD-Score:-3.86|P-value:5.68E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR; predicted to have a role in transcription based on computational guilt by association analysis Gene:YKL069W(YKL069W)|FD-Score:-3.21|P-value:6.56E-4||SGD DESC:Methionine-R-sulfoxide reductase; reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress; relative distribution to the nucleus increases upon DNA replication stress Gene:YKR075C(YKR075C_p)|FD-Score:-4.18|P-value:1.45E-5||SGD DESC:Protein of unknown function; similar to YOR062Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YLR241W(YLR241W_p)|FD-Score:-3.18|P-value:7.38E-4||SGD DESC:Putative protein of unknown function, may be involved in detoxification Gene:YLR294C(YLR294C_d)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ATP14 Gene:YLR358C(YLR358C_p)|FD-Score:4.33|P-value:7.61E-6||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps ORF RSC2/YLR357W Gene:YMR247W-A(YMR247W-A_p)|FD-Score:-3.75|P-value:8.72E-5||SGD DESC:Putative protein of unknown function Gene:YMR279C(YMR279C)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Putative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; YMR279C has a paralog, ATR1, that arose from the whole genome duplication Gene:YNL120C(YNL120C_d)|FD-Score:3.33|P-value:4.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion enhances replication of Brome mosaic virus in S. cerevisiae, but likely due to effects on the overlapping gene Gene:YNR066C(YNR066C_p)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:Putative membrane-localized protein of unknown function Gene:YOL037C(YOL037C_d)|FD-Score:-3.24|P-value:5.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOL036W Gene:YPL257W(YPL257W_p)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene Gene:YPL261C(YPL261C_d)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YPL260W Gene:YPR096C(YPR096C)|FD-Score:4.22|P-value:1.25E-5||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR192W8.471.23E-171.65RIM2Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
YOL144W7.669.36E-151.65NOP8Nucleolar protein required for 60S ribosomal subunit biogenesis
YDR238C6.019.31E-100.31SEC26Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP)
YPL122C5.705.88E-90.08TFB2Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH
YMR290W-A_d5.639.14E-90.47YMR290W-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase
YER127W5.161.25E-70.08LCP5Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus
YPL153C5.081.86E-70.10RAD53Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication
YIL026C4.983.10E-70.11IRR1Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability
YDR373W4.875.50E-70.21FRQ1N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily
YER168C4.661.58E-60.06CCA1ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites
YDR473C4.602.15E-60.83PRP3Splicing factor, component of the U4/U6-U5 snRNP complex
YGL238W3.768.35E-50.13CSE1Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation
YEL055C3.641.38E-40.14POL5DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA
YOL120C3.502.37E-40.03RPL18ARibosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication
YLR153C3.472.60E-40.07ACS2Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YFL016C6.321.30E-10MDJ1Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones
YPL051W5.891.98E-9ARL3GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor
YNL202W5.591.13E-8SPS19Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate
YMR306W5.581.20E-8FKS3Protein involved in spore wall assembly, has similarity to 1,3-beta-D-glucan synthase catalytic subunits Fks1p and Gsc2p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YMR311C5.267.29E-8GLC8Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress
YPL219W5.257.43E-8PCL8Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation
YGR038W5.111.57E-7ORM1Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control
YER078C4.454.37E-6ICP55Mitochondrial aminopeptidase; cleaves the N termini of at least 38 imported proteins after cleavage by the mitochondrial processing peptidase (MPP), thereby increasing their stability; member of the aminopeptidase P family
YBR057C4.434.61E-6MUM2Cytoplasmic protein essential for meiotic DNA replication and sporulation; interacts with Orc2p, which is a component of the origin recognition complex
YLR263W4.366.64E-6RED1Protein component of the synaptonemal complex axial elements, involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; interacts with Hop1p, Mec3p and Ddc1p
YLR358C_p4.337.61E-6YLR358C_pProtein of unknown function; expressed at both mRNA and protein levels; partially overlaps ORF RSC2/YLR357W
YPR096C4.221.25E-5YPR096CProtein of unknown function that may interact with ribosomes, based on co-purification experiments
YPL257W_p4.211.30E-5YPL257W_pPutative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene
YDR538W4.151.67E-5PAD1Phenylacrylic acid decarboxylase, confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX
YFL001W4.131.80E-5DEG1tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability

GO enrichment analysis for SGTC_2943
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1128.70E-18SGTC_7301436-0052 41.2 μMChemDiv (Drug-like library)7687640.153846mitochondrial processes
0.0984.68E-14SGTC_29429052777 62.2 μMChembridge (Drug-like library)64695030.333333
0.0863.42E-11SGTC_8510394-0702 453.0 μMChemDiv (Drug-like library)7626460.0597015mitochondrial processes
0.0723.47E-8SGTC_31969111891 49.5 μMChembridge (Drug-like library)188915100.1625mitochondrial processes
0.0707.29E-8SGTC_623k072-0108 16.9 μMChemDiv (Drug-like library)53828030.0895522heme biosynthesis & mitochondrial translocase
0.0681.49E-7SGTC_24595811157 9.2 μMMiscellaneous28706840.163934mitochondrial processes
0.0681.70E-7SGTC_1796st049173 110.4 μMTimTec (Natural product derivative library)941340.107143
0.0672.28E-7SGTC_630099-0242 26.7 μMChemDiv (Drug-like library)38985020.0491803mitochondrial stress
0.0673.09E-7SGTC_30579095946 49.5 μMChembridge (Drug-like library)245400430.15942Golgi
0.0648.67E-7SGTC_1098clofazimine 15.1 μMNIH Clinical Collection27940.113924heme biosynthesis & mitochondrial translocase
0.0621.73E-6SGTC_9691313-0238 134.0 μMChemDiv (Drug-like library)52128740.191176mitochondrial processes
0.0621.95E-6SGTC_21956536160 144.2 μMChembridge (Fragment library)38343860.086206960S ribosome export
0.0612.37E-6SGTC_12470448-0043 5.8 μMChemDiv (Drug-like library)30890130.0895522ERG2
0.0612.53E-6SGTC_31819108277 49.5 μMChembridge (Drug-like library)170972980.269841iron homeostasis
0.0604.64E-6SGTC_21836420625 130.5 μMChembridge (Fragment library)28976190.132353mitochondrial processes

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1462k081-001426.5 μM13270634ChemDiv (Drug-like library)329.5521833.08213
SGTC_1035k081-004155.2 μM0.680851731709ChemDiv (Drug-like library)341.58772.8613Golgi
SGTC_2941905614511.23 μM0.6808512119517Chembridge (Drug-like library)379.559693.81915
SGTC_3010908008971.43 μM0.59615417027233Chembridge (Drug-like library)378.2387833.49714
SGTC_1463k081-003234.1 μM0.571429977755ChemDiv (Drug-like library)345.114633.15415
SGTC_2967908953849.85 μM0.444444728216Chembridge (Drug-like library)300.663633.0715
SGTC_14524194-0072190 μM0.4285711343545ChemDiv (Drug-like library)408.248064.14914
SGTC_21876125751200 μM0.411765874498Chembridge (Fragment library)235.1895861.15215RSC complex & mRNA processing
SGTC_3283910933235.82 μM0.38709717027281Chembridge (Drug-like library)329.82393.45913DNA intercalators
SGTC_8480368-008985.6 μM0.384615762535ChemDiv (Drug-like library)267.110722.79212iron homeostasis