Deletion Strain | FD score | P-value | Clearance | Gene | Gene Description |
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YDL145C | 7.59 | 1.62E-14 | 0.99 | COP1 | Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway |
YER127W | 6.95 | 1.85E-12 | 0.99 | LCP5 | Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus |
YIR022W | 6.86 | 3.38E-12 | 0.99 | SEC11 | 18kDa catalytic subunit of the Signal Peptidase Complex (SPC; Spc1p, Spc2p, Spc3p, and Sec11p) which cleaves the signal sequence of proteins targeted to the endoplasmic reticulum |
YOR056C | 5.87 | 2.17E-9 | 0.88 | NOB1 | Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress |
YOR217W | 4.99 | 2.96E-7 | 0.24 | RFC1 | Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon |
YAR007C | 4.75 | 1.02E-6 | 0.13 | RFA1 | Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination |
YDL220C | 4.62 | 1.89E-6 | 0.10 | CDC13 | Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation |
YLR071C | 4.53 | 3.02E-6 | 0.27 | RGR1 | Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for glucose repression, HO repression, RME1 repression and sporulation |
YGR114C_d | 4.26 | 1.02E-5 | 0.03 | YGR114C_d | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 |
YDL193W | 4.23 | 1.19E-5 | 0.05 | NUS1 | Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY |
YOR146W_d | 4.17 | 1.50E-5 | 0.03 | YOR146W_d | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C |
YMR131C | 4.15 | 1.69E-5 | 0.20 | RRB1 | Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p |
YLR163C | 3.95 | 3.94E-5 | 0.11 | MAS1 | Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins |
YPR088C | 3.84 | 6.20E-5 | 0.17 | SRP54 | Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain |
YDL029W | 3.67 | 1.20E-4 | 0.06 | ARP2 | Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity |