k015-0027

1-[(4-methylphenyl)methyl]-5-nitroindole-2,3-dione

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_295
Screen concentration 31.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 3887424
SMILES CC1=CC=C(C=C1)CN2C3=C(C=C(C=C3)[N+](=O)[O-])C(=O)C2=O
Standardized SMILES Cc1ccc(CN2C(=O)C(=O)c3cc(ccc23)[N+](=O)[O-])cc1
Molecular weight 296.2775
ALogP 2.84
H-bond donor count 0
H-bond acceptor count 4
Response signature PDR1

Pool Growth Kinetics
% growth inhibition (Het. pool) 16.59
% growth inhibition (Hom. pool) 8.51


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3887424
Download HIP data (tab-delimited text)  (excel)
Gene:RPA190(YOR341W)|FD-Score:3.15|P-value:8.25E-4|Clearance:0.12||SGD DESC:RNA polymerase I largest subunit A190 Gene:RPO26(YPR187W)|FD-Score:3.3|P-value:4.90E-4|Clearance:0.12||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RRP4(YHR069C)|FD-Score:3.57|P-value:1.80E-4|Clearance:0.05||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:SPC105(YGL093W)|FD-Score:4.18|P-value:1.44E-5|Clearance:0.61||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:TOP2(YNL088W)|FD-Score:3.17|P-value:7.51E-4|Clearance:0.03||SGD DESC:Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:TSR4(YOL022C)|FD-Score:-3.61|P-value:1.55E-4|Clearance:0||SGD DESC:Cytoplasmic protein required for correct processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; essential gene in S288C background but not in CEN.PK2 Gene:YAE1(YJR067C)|FD-Score:-3.55|P-value:1.96E-4|Clearance:0||SGD DESC:Protein that forms a complex with Lto1p and Rli1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species Gene:YDR327W(YDR327W_d)|FD-Score:3.52|P-value:2.15E-4|Clearance:0.22||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SKP1 Gene:RPA190(YOR341W)|FD-Score:3.15|P-value:8.25E-4|Clearance:0.12||SGD DESC:RNA polymerase I largest subunit A190 Gene:RPO26(YPR187W)|FD-Score:3.3|P-value:4.90E-4|Clearance:0.12||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RRP4(YHR069C)|FD-Score:3.57|P-value:1.80E-4|Clearance:0.05||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:SPC105(YGL093W)|FD-Score:4.18|P-value:1.44E-5|Clearance:0.61||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:TOP2(YNL088W)|FD-Score:3.17|P-value:7.51E-4|Clearance:0.03||SGD DESC:Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:TSR4(YOL022C)|FD-Score:-3.61|P-value:1.55E-4|Clearance:0||SGD DESC:Cytoplasmic protein required for correct processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; essential gene in S288C background but not in CEN.PK2 Gene:YAE1(YJR067C)|FD-Score:-3.55|P-value:1.96E-4|Clearance:0||SGD DESC:Protein that forms a complex with Lto1p and Rli1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species Gene:YDR327W(YDR327W_d)|FD-Score:3.52|P-value:2.15E-4|Clearance:0.22||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SKP1

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3887424
Download HOP data (tab-delimited text)  (excel)
Gene:AAP1(YHR047C)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication Gene:ACE2(YLR131C)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:BRO1(YPL084W)|FD-Score:3.24|P-value:5.93E-4||SGD DESC:Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes Gene:CWC21(YDR482C)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Protein involved in RNA splicing by the spliceosome; component of a complex containing Cef1p; interacts genetically with ISY1 and BUD13; may bind RNA; has similarity to S. pombe Cwf21p Gene:DBF2(YGR092W)|FD-Score:3.78|P-value:7.87E-5||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:FMT1(YBL013W)|FD-Score:3.12|P-value:8.92E-4||SGD DESC:Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate Gene:FRA2(YGL220W)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:GMC2(YLR445W)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; transcription is regulated by Ume6p and induced in response to alpha factor Gene:GUD1(YDL238C)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures Gene:HFI1(YPL254W)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:MAC1(YMR021C)|FD-Score:4.41|P-value:5.07E-6||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MET31(YPL038W)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET31 has a paralog, MET32, that arose from the whole genome duplication Gene:MON2(YNL297C)|FD-Score:-3.31|P-value:4.74E-4||SGD DESC:Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins Gene:MRH1(YDR033W)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Protein that localizes primarily to the plasma membrane; also found at the nuclear envelope; the authentic, non-tagged protein is detected in mitochondria in a phosphorylated state; MRH1 has a paralog, YRO2, that arose from the whole genome duplication Gene:MSO1(YNR049C)|FD-Score:3.18|P-value:7.38E-4||SGD DESC:Probable component of the secretory vesicle docking complex; acts at a late step in secretion; shows genetic and physical interactions with Sec1p; required for prospore membrane formation during sporulation; relocalizes from bud neck to nucleus upon DNA replication stress Gene:NCR1(YPL006W)|FD-Score:-3.52|P-value:2.15E-4||SGD DESC:Vacuolar membrane protein that transits through the biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; glycoprotein and functional orthologue of human Niemann Pick C1 (NPC1) protein Gene:PDR1(YGL013C)|FD-Score:17.4|P-value:1.72E-68||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PEX28(YHR150W)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Peroxisomal integral membrane peroxin, involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p Gene:PGD1(YGL025C)|FD-Score:6.78|P-value:6.11E-12||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PHO87(YCR037C)|FD-Score:-3.47|P-value:2.60E-4||SGD DESC:Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication Gene:PHO89(YBR296C)|FD-Score:5.37|P-value:3.87E-8||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:PTR2(YKR093W)|FD-Score:3.76|P-value:8.52E-5||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:RPL16B(YNL069C)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Ribosomal 60S subunit protein L16B; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16B has a paralog, RPL16A, that arose from the whole genome duplication Gene:RSM25(YIL093C)|FD-Score:4.12|P-value:1.86E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RTN2(YDL204W)|FD-Score:3.33|P-value:4.42E-4||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication Gene:SDC1(YDR469W)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 Gene:SPT2(YER161C)|FD-Score:3.15|P-value:8.04E-4||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:SPT3(YDR392W)|FD-Score:4.82|P-value:7.31E-7||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SWT21(YNL187W)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Protein involved in mRNA splicing; contains a consensus nuclear export signal (NES) sequence similar to the consensus sequence recognized by Crm1p; interacts genetically with Prp40p and Tgs1p; contains WD40 repeats Gene:TAE2(YPL009C)|FD-Score:3.75|P-value:8.98E-5||SGD DESC:Protein of unknown function, involved in protein translation; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:TAT2(YOL020W)|FD-Score:3.28|P-value:5.15E-4||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TOS2(YGR221C)|FD-Score:3.15|P-value:8.14E-4||SGD DESC:Protein involved in localization of Cdc24p to the site of bud growth; may act as a membrane anchor; localizes to the bud neck and bud tip; potentially phosphorylated by Cdc28p Gene:YDL177C(YDL177C_p)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Putative protein of unknown function; similar to the mouse IMPACT gene; YDL177C is not an essential gene Gene:YGL006W-A(YGL006W-A_p)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YGL188C-A(YGL188C-A_p)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Putative protein of unknown function Gene:YMR105W-A(YMR105W-A_p)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Putative protein of unknown function Gene:YOL050C(YOL050C_d)|FD-Score:6.01|P-value:9.18E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:AAP1(YHR047C)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication Gene:ACE2(YLR131C)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:BRO1(YPL084W)|FD-Score:3.24|P-value:5.93E-4||SGD DESC:Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes Gene:CWC21(YDR482C)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Protein involved in RNA splicing by the spliceosome; component of a complex containing Cef1p; interacts genetically with ISY1 and BUD13; may bind RNA; has similarity to S. pombe Cwf21p Gene:DBF2(YGR092W)|FD-Score:3.78|P-value:7.87E-5||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:FMT1(YBL013W)|FD-Score:3.12|P-value:8.92E-4||SGD DESC:Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate Gene:FRA2(YGL220W)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:GMC2(YLR445W)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; transcription is regulated by Ume6p and induced in response to alpha factor Gene:GUD1(YDL238C)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures Gene:HFI1(YPL254W)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:MAC1(YMR021C)|FD-Score:4.41|P-value:5.07E-6||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MET31(YPL038W)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET31 has a paralog, MET32, that arose from the whole genome duplication Gene:MON2(YNL297C)|FD-Score:-3.31|P-value:4.74E-4||SGD DESC:Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins Gene:MRH1(YDR033W)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Protein that localizes primarily to the plasma membrane; also found at the nuclear envelope; the authentic, non-tagged protein is detected in mitochondria in a phosphorylated state; MRH1 has a paralog, YRO2, that arose from the whole genome duplication Gene:MSO1(YNR049C)|FD-Score:3.18|P-value:7.38E-4||SGD DESC:Probable component of the secretory vesicle docking complex; acts at a late step in secretion; shows genetic and physical interactions with Sec1p; required for prospore membrane formation during sporulation; relocalizes from bud neck to nucleus upon DNA replication stress Gene:NCR1(YPL006W)|FD-Score:-3.52|P-value:2.15E-4||SGD DESC:Vacuolar membrane protein that transits through the biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; glycoprotein and functional orthologue of human Niemann Pick C1 (NPC1) protein Gene:PDR1(YGL013C)|FD-Score:17.4|P-value:1.72E-68||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PEX28(YHR150W)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Peroxisomal integral membrane peroxin, involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p Gene:PGD1(YGL025C)|FD-Score:6.78|P-value:6.11E-12||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PHO87(YCR037C)|FD-Score:-3.47|P-value:2.60E-4||SGD DESC:Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication Gene:PHO89(YBR296C)|FD-Score:5.37|P-value:3.87E-8||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:PTR2(YKR093W)|FD-Score:3.76|P-value:8.52E-5||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:RPL16B(YNL069C)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Ribosomal 60S subunit protein L16B; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16B has a paralog, RPL16A, that arose from the whole genome duplication Gene:RSM25(YIL093C)|FD-Score:4.12|P-value:1.86E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RTN2(YDL204W)|FD-Score:3.33|P-value:4.42E-4||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication Gene:SDC1(YDR469W)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 Gene:SPT2(YER161C)|FD-Score:3.15|P-value:8.04E-4||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:SPT3(YDR392W)|FD-Score:4.82|P-value:7.31E-7||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SWT21(YNL187W)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Protein involved in mRNA splicing; contains a consensus nuclear export signal (NES) sequence similar to the consensus sequence recognized by Crm1p; interacts genetically with Prp40p and Tgs1p; contains WD40 repeats Gene:TAE2(YPL009C)|FD-Score:3.75|P-value:8.98E-5||SGD DESC:Protein of unknown function, involved in protein translation; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:TAT2(YOL020W)|FD-Score:3.28|P-value:5.15E-4||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TOS2(YGR221C)|FD-Score:3.15|P-value:8.14E-4||SGD DESC:Protein involved in localization of Cdc24p to the site of bud growth; may act as a membrane anchor; localizes to the bud neck and bud tip; potentially phosphorylated by Cdc28p Gene:YDL177C(YDL177C_p)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Putative protein of unknown function; similar to the mouse IMPACT gene; YDL177C is not an essential gene Gene:YGL006W-A(YGL006W-A_p)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YGL188C-A(YGL188C-A_p)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Putative protein of unknown function Gene:YMR105W-A(YMR105W-A_p)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Putative protein of unknown function Gene:YOL050C(YOL050C_d)|FD-Score:6.01|P-value:9.18E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL093W4.181.44E-50.61SPC105Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components
YHR069C3.571.80E-40.05RRP4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2)
YDR327W_d3.522.15E-40.23YDR327W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SKP1
YPR187W3.304.90E-40.12RPO26RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit
YNL088W3.177.51E-40.03TOP2Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation
YOR341W3.158.25E-40.12RPA190RNA polymerase I largest subunit A190
YNL207W3.030.001240.14RIO2Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p
YHR062C2.890.001950.11RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YER165W2.780.002710.11PAB1Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G
YLR274W2.670.003790.04MCM5Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase
YBL023C2.630.004290.03MCM2Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress
YLR316C2.600.004680.01TAD3Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad2p that converts adenosine to inosine at the wobble position of several tRNAs
YGR145W2.590.004770.07ENP2Essential nucleolar protein, required for biogenesis of the small ribosomal subunit; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p
YIL150C2.520.005850.05MCM10Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins
YLR071C2.470.006725.95E-4RGR1Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for glucose repression, HO repression, RME1 repression and sporulation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL013C17.401.72E-68PDR1Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
YGL025C6.786.11E-12PGD1Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor
YOL050C_d6.019.18E-10YOL050C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
YBR296C5.373.87E-8PHO89Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification
YDR392W4.827.31E-7SPT3Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters
YMR021C4.415.07E-6MAC1Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport
YIL093C4.121.86E-5RSM25Mitochondrial ribosomal protein of the small subunit
YGL006W-A_p4.062.46E-5YGL006W-A_pPutative protein of unknown function; identified by SAGE
YGR092W3.787.87E-5DBF2Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis
YKR093W3.768.52E-5PTR2Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p
YPL009C3.758.98E-5TAE2Protein of unknown function, involved in protein translation; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YGL188C-A_p3.691.12E-4YGL188C-A_pPutative protein of unknown function
YDL177C_p3.631.41E-4YDL177C_pPutative protein of unknown function; similar to the mouse IMPACT gene; YDL177C is not an essential gene
YNL187W3.611.53E-4SWT21Protein involved in mRNA splicing; contains a consensus nuclear export signal (NES) sequence similar to the consensus sequence recognized by Crm1p; interacts genetically with Prp40p and Tgs1p; contains WD40 repeats
YHR150W3.522.17E-4PEX28Peroxisomal integral membrane peroxin, involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p

GO enrichment analysis for SGTC_295
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.4231.02E-254SGTC_1700st030840 7.9 μMTimTec (Natural product derivative library)54993070.170732PDR1
0.3795.43E-201SGTC_6641222-0054 14.9 μMChemDiv (Drug-like library)68139910.114286sphingolipid biosynthesis & PDR1
0.3531.32E-172SGTC_250brefeldin a 552.2 μMICCB bioactive library64361870.0666667PDR1
0.3518.74E-171SGTC_32419133421 49.5 μMChembridge (Drug-like library)170182210.0930233PDR1
0.3373.57E-157SGTC_1694st024011 44.9 μMTimTec (Natural product derivative library)27875330.0697674ERG2
0.3315.06E-151SGTC_12430416-0003 5.7 μMChemDiv (Drug-like library)21419770.0491803PDR1
0.3161.22E-136SGTC_12770873-0036 22.0 μMChemDiv (Drug-like library)59657350.0684932PDR1
0.3111.71E-132SGTC_5274476-4215 268.0 μMChemDiv (Drug-like library)44187540.0645161PDR1
0.2951.70E-118SGTC_1139k015-0024 46.9 μMChemDiv (Drug-like library)27666520.357143PDR1
0.2692.11E-98SGTC_5983937-0236 20.2 μMChemDiv (Drug-like library)13793130.203125ERG2
0.2576.24E-90SGTC_2508celastrol 40.5 μMMicrosource (Natural product library)1227240.0689655PDR1
0.2452.44E-81SGTC_505gw-5074 63.9 μMICCB bioactive library59242080.1
0.2434.15E-80SGTC_30259087895 49.5 μMChembridge (Drug-like library)171736410.0864198PDR1
0.2221.06E-66SGTC_687k029-0044 34.9 μMChemDiv (Drug-like library)58293380.05PDR1
0.2212.39E-66SGTC_489paxilline 115.0 μMICCB bioactive library1050080.0638298PDR1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1028k015-00294.8 μM0.7021284588309ChemDiv (Drug-like library)351.141023.68304
SGTC_196k015-002318.11 μM0.6956525211998ChemDiv (Drug-like library)361.146963.10204PDR1
SGTC_1027k015-00258.67 μM0.5882353598955ChemDiv (Drug-like library)361.146963.10204
SGTC_1457k015-003257.3 μM0.5555564302660ChemDiv (Drug-like library)334.6864233.22405
SGTC_169k015-001716.03 μM0.481840002ChemDiv (Drug-like library)334.1398633.41303
SGTC_1000086-012849.59 μM0.43137393297ChemDiv (Drug-like library)248.234682.08504copper-dependent oxidative stress
SGTC_544k015-002052.4 μM0.4285713008304ChemDiv (Drug-like library)316.14943.20802
SGTC_1458k015-00346.33 μM0.4230773598957ChemDiv (Drug-like library)352.1303263.61904
SGTC_13031160-0052261 μM0.43562476ChemDiv (Drug-like library)281.266142.87413
SGTC_3710195-00067.36 μM0.3913043928593ChemDiv (Drug-like library)253.209682.70204