9073731

N-(4-chlorophenyl)-2-(4-ethyl-2-oxomorpholin-3-yl)acetamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2952
Screen concentration 13.8 μM
Source Chembridge (Drug-like library)
PubChem CID 16457353
SMILES CCN1CCOC(=O)C1CC(=O)NC2=CC=C(C=C2)Cl
Standardized SMILES CCN1CCOC(=O)C1CC(=O)Nc2ccc(Cl)cc2
Molecular weight 296.7494
ALogP 1.88
H-bond donor count 1
H-bond acceptor count 4
Response signature tubulin folding & SWR complex

Pool Growth Kinetics
% growth inhibition (Het. pool) 5.02
% growth inhibition (Hom. pool) 1.25


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 16457353
Download HIP data (tab-delimited text)  (excel)
Gene:ARC15(YIL062C)|FD-Score:3.23|P-value:6.26E-4|Clearance:0.01||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; has mRNA binding activity Gene:ARP9(YMR033W)|FD-Score:3.13|P-value:8.75E-4|Clearance:0.07||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CAB2(YIL083C)|FD-Score:4.79|P-value:8.18E-7|Clearance:0.02||SGD DESC:Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity) Gene:CCT7(YJL111W)|FD-Score:4.13|P-value:1.85E-5|Clearance:0.06||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:ECM16(YMR128W)|FD-Score:4.21|P-value:1.26E-5|Clearance:0.09||SGD DESC:Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis Gene:GCD1(YOR260W)|FD-Score:5.88|P-value:2.09E-9|Clearance:0.18||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:HSF1(YGL073W)|FD-Score:-6.09|P-value:5.50E-10|Clearance:0||SGD DESC:Trimeric heat shock transcription factor, activates multiple genes in response to stresses that include hyperthermia; recognizes variable heat shock elements (HSEs) consisting of inverted NGAAN repeats; posttranslationally regulated Gene:OLE1(YGL055W)|FD-Score:4.06|P-value:2.44E-5|Clearance:0.04||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:RAD53(YPL153C)|FD-Score:5.69|P-value:6.22E-9|Clearance:0.39||SGD DESC:Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication Gene:RET1(YOR207C)|FD-Score:7.66|P-value:9.61E-15|Clearance:0.64||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPB10(YOR210W)|FD-Score:4.77|P-value:9.24E-7|Clearance:0.42||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPP0(YLR340W)|FD-Score:-4.14|P-value:1.74E-5|Clearance:0||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:RPP1(YHR062C)|FD-Score:7.02|P-value:1.11E-12|Clearance:1.14||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS13(YDR064W)|FD-Score:5.3|P-value:5.82E-8|Clearance:0.45||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:RSC58(YLR033W)|FD-Score:3.37|P-value:3.73E-4|Clearance:0.15||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SRP21(YKL122C)|FD-Score:3.21|P-value:6.54E-4|Clearance:0.08||SGD DESC:Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm Gene:TSC10(YBR265W)|FD-Score:4.02|P-value:2.92E-5|Clearance:0.65||SGD DESC:3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family Gene:YJL195C(YJL195C_d)|FD-Score:4.35|P-value:6.89E-6|Clearance:0.13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YPL142C(YPL142C_d)|FD-Score:-5.44|P-value:2.64E-8|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit Gene:YPR142C(YPR142C_d)|FD-Score:4.85|P-value:6.16E-7|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing Gene:YTM1(YOR272W)|FD-Score:9.77|P-value:7.31E-23|Clearance:2.12||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats Gene:ARC15(YIL062C)|FD-Score:3.23|P-value:6.26E-4|Clearance:0.01||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; has mRNA binding activity Gene:ARP9(YMR033W)|FD-Score:3.13|P-value:8.75E-4|Clearance:0.07||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CAB2(YIL083C)|FD-Score:4.79|P-value:8.18E-7|Clearance:0.02||SGD DESC:Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity) Gene:CCT7(YJL111W)|FD-Score:4.13|P-value:1.85E-5|Clearance:0.06||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:ECM16(YMR128W)|FD-Score:4.21|P-value:1.26E-5|Clearance:0.09||SGD DESC:Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis Gene:GCD1(YOR260W)|FD-Score:5.88|P-value:2.09E-9|Clearance:0.18||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:HSF1(YGL073W)|FD-Score:-6.09|P-value:5.50E-10|Clearance:0||SGD DESC:Trimeric heat shock transcription factor, activates multiple genes in response to stresses that include hyperthermia; recognizes variable heat shock elements (HSEs) consisting of inverted NGAAN repeats; posttranslationally regulated Gene:OLE1(YGL055W)|FD-Score:4.06|P-value:2.44E-5|Clearance:0.04||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:RAD53(YPL153C)|FD-Score:5.69|P-value:6.22E-9|Clearance:0.39||SGD DESC:Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication Gene:RET1(YOR207C)|FD-Score:7.66|P-value:9.61E-15|Clearance:0.64||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPB10(YOR210W)|FD-Score:4.77|P-value:9.24E-7|Clearance:0.42||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPP0(YLR340W)|FD-Score:-4.14|P-value:1.74E-5|Clearance:0||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:RPP1(YHR062C)|FD-Score:7.02|P-value:1.11E-12|Clearance:1.14||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS13(YDR064W)|FD-Score:5.3|P-value:5.82E-8|Clearance:0.45||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:RSC58(YLR033W)|FD-Score:3.37|P-value:3.73E-4|Clearance:0.15||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SRP21(YKL122C)|FD-Score:3.21|P-value:6.54E-4|Clearance:0.08||SGD DESC:Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm Gene:TSC10(YBR265W)|FD-Score:4.02|P-value:2.92E-5|Clearance:0.65||SGD DESC:3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family Gene:YJL195C(YJL195C_d)|FD-Score:4.35|P-value:6.89E-6|Clearance:0.13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YPL142C(YPL142C_d)|FD-Score:-5.44|P-value:2.64E-8|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit Gene:YPR142C(YPR142C_d)|FD-Score:4.85|P-value:6.16E-7|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing Gene:YTM1(YOR272W)|FD-Score:9.77|P-value:7.31E-23|Clearance:2.12||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 16457353
Download HOP data (tab-delimited text)  (excel)
Gene:ADP1(YCR011C)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Putative ATP-dependent permease of the ABC transporter family of proteins Gene:AIM43(YPL099C)|FD-Score:-3.26|P-value:5.62E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:AIP1(YMR092C)|FD-Score:-3.69|P-value:1.14E-4||SGD DESC:Actin cortical patch component; interacts with the actin depolymerizing factor cofilin; required to restrict cofilin localization to cortical patches; contains WD repeats; protein increases in abundance and relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:APL6(YGR261C)|FD-Score:-3.27|P-value:5.46E-4||SGD DESC:Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools Gene:ARP6(YLR085C)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:ATG33(YLR356W)|FD-Score:4.33|P-value:7.34E-6||SGD DESC:Mitochondrial mitophagy-specific protein; required primarily for mitophagy induced at the post-log phase; not required for other types of selective autophagy or macroautophagy; conserved within fungi, but not in higher eukaryotes Gene:ATP7(YKL016C)|FD-Score:3.83|P-value:6.35E-5||SGD DESC:Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:AVT4(YNL101W)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BDS1(YOL164W)|FD-Score:3.98|P-value:3.46E-5||SGD DESC:Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources Gene:CBT1(YKL208W)|FD-Score:-3.53|P-value:2.07E-4||SGD DESC:Protein involved in 5' end processing of mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p Gene:CCS1(YMR038C)|FD-Score:7.28|P-value:1.67E-13||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CDC55(YGL190C)|FD-Score:3.88|P-value:5.14E-5||SGD DESC:Non-essential regulatory subunit B of protein phosphatase 2A (PP2A); localization to the cytoplasm requires Zds1p and Zds2p and promotes mitotic entry; localization to the nucleus prevents mitotic exit; required for correct nuclear division and chromosome segregation in meiosis; maintains nucleolar sequestration of Cdc14p during early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion Gene:CDC73(YLR418C)|FD-Score:4.55|P-value:2.64E-6||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CGI121(YML036W)|FD-Score:5.38|P-value:3.71E-8||SGD DESC:Component of the EKC/KEOPS complex with Bud32p, Kae1p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription; Cgi121p is dispensable for tRNA modification Gene:CHS3(YBR023C)|FD-Score:3.4|P-value:3.31E-4||SGD DESC:Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan Gene:CHS7(YHR142W)|FD-Score:3.78|P-value:7.80E-5||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:CIN1(YOR349W)|FD-Score:7.94|P-value:1.03E-15||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:CLA4(YNL298W)|FD-Score:6.01|P-value:9.49E-10||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CLB6(YGR109C)|FD-Score:-4|P-value:3.14E-5||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 Gene:CRG1(YHR209W)|FD-Score:3.75|P-value:8.86E-5||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:CSF1(YLR087C)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CSM1(YCR086W)|FD-Score:4.49|P-value:3.48E-6||SGD DESC:Nucleolar protein that forms a complex with Lrs4p and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:CTL1(YMR180C)|FD-Score:-3.32|P-value:4.54E-4||SGD DESC:RNA 5'-triphosphatase, localizes to both the nucleus and cytoplasm Gene:CTP1(YBR291C)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Mitochondrial inner membrane citrate transporter, member of the mitochondrial carrier family Gene:CTR1(YPR124W)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CUE1(YMR264W)|FD-Score:-3.18|P-value:7.26E-4||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:CUE5(YOR042W)|FD-Score:4.26|P-value:1.00E-5||SGD DESC:Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DDI1(YER143W)|FD-Score:4.39|P-value:5.54E-6||SGD DESC:DNA damage-inducible v-SNARE binding protein with a role in suppression of protein secretion; may play a role in S-phase checkpoint control; has ubiquitin-associated (UBA), ubiquitin-like (UBL), and retroviral-like proteinase (RVP) domains Gene:DSS4(YPR017C)|FD-Score:4.97|P-value:3.43E-7||SGD DESC:Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol Gene:ECM18(YDR125C)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication Gene:FAR3(YMR052W)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:FPR4(YLR449W)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase) (proline isomerase) localized to the nucleus; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones Gene:FYV7(YLR068W)|FD-Score:-3.15|P-value:8.20E-4||SGD DESC:Essential protein required for maturation of 18S rRNA; required for survival upon exposure to K1 killer toxin Gene:GND1(YHR183W)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication Gene:GND2(YGR256W)|FD-Score:-4.02|P-value:2.86E-5||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone; GND2 has a paralog, GND1, that arose from the whole genome duplication Gene:HTZ1(YOL012C)|FD-Score:3.15|P-value:8.04E-4||SGD DESC:Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin Gene:ITC1(YGL133W)|FD-Score:-3.27|P-value:5.43E-4||SGD DESC:Subunit of the ATP-dependent Isw2p-Itc1p chromatin remodeling complex, required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex Gene:JHD2(YJR119C)|FD-Score:-3.34|P-value:4.14E-4||SGD DESC:JmjC domain family histone demethylase specific for H3-K4 (histone H3 Lys4); removes methyl groups specifically added by Set1p methyltransferase; protein levels regulated by Not4p (E3 ubiquitin ligase) polyubiquitin-mediated degradation Gene:KRE2(YDR483W)|FD-Score:4.45|P-value:4.31E-6||SGD DESC:Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication Gene:LAA1(YJL207C)|FD-Score:-3.34|P-value:4.13E-4||SGD DESC:AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene Gene:LAG2(YOL025W)|FD-Score:3.15|P-value:8.16E-4||SGD DESC:Protein that negatively regulates the SCF E3-ubiquitin ligase by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1 Gene:LTE1(YAL024C)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MAD1(YGL086W)|FD-Score:-3.7|P-value:1.10E-4||SGD DESC:Coiled-coil protein involved in the spindle-assembly checkpoint; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon spindle assembly checkpoint arrest; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of the activity of the anaphase promoting complex; forms a complex with Mad2p Gene:MDG1(YNL173C)|FD-Score:4.01|P-value:3.06E-5||SGD DESC:Plasma membrane protein involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations Gene:MET22(YOL064C)|FD-Score:-3.11|P-value:9.36E-4||SGD DESC:Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway Gene:MID2(YLR332W)|FD-Score:4|P-value:3.12E-5||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:MMR1(YLR190W)|FD-Score:-3.91|P-value:4.64E-5||SGD DESC:Phosphorylated protein of the mitochondrial outer membrane, localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p Gene:MNE1(YOR350C)|FD-Score:4.39|P-value:5.67E-6||SGD DESC:Mitochondrial matrix protein involved in splicing Group I aI5-beta intron from COX1 mRNA Gene:MNN10(YDR245W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family Gene:MOS1(YCL057C-A)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Mitochondrial protein essential for proper inner membrane organization; conserved component of the mitochondrial inner membrane organizing system (MICOS, MINOS, or MitOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture; ortholog of human MINOS1 Gene:MSA2(YKR077W)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation Gene:MUD1(YBR119W)|FD-Score:3.75|P-value:8.78E-5||SGD DESC:U1 snRNP A protein, homolog of human U1-A; involved in nuclear mRNA splicing Gene:MXR2(YCL033C)|FD-Score:5.37|P-value:3.95E-8||SGD DESC:Methionine-R-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR1; involved in the regulation of lifespan Gene:NBP2(YDR162C)|FD-Score:4.59|P-value:2.20E-6||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NKP2(YLR315W)|FD-Score:4.41|P-value:5.27E-6||SGD DESC:Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2 Gene:NPL3(YDR432W)|FD-Score:4.07|P-value:2.35E-5||SGD DESC:RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; has a role in repressing translation initiation by binding eIF4G; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm; protein abundance increases in response to DNA replication stress Gene:OM14(YBR230C)|FD-Score:-3.84|P-value:6.07E-5||SGD DESC:Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron Gene:OSH6(YKR003W)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Member of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion Gene:PAC2(YER007W)|FD-Score:5.6|P-value:1.06E-8||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PET122(YER153C)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet494p; located in the mitochondrial inner membrane Gene:PET309(YLR067C)|FD-Score:3.1|P-value:9.78E-4||SGD DESC:Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains seven pentatricopeptide repeats (PPRs) Gene:PHM8(YER037W)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Lysophosphatidic acid (LPA) phosphatase involved in LPA hydrolysis in response to phosphate starvation; phosphatase activity is soluble and Mg2+ dependent; expression is induced by low phosphate levels and by inactivation of Pho85p Gene:PPT2(YPL148C)|FD-Score:-3.59|P-value:1.64E-4||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PRO2(YOR323C)|FD-Score:-3.97|P-value:3.59E-5||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RCF1(YML030W)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Cytochrome c oxidase subunit; required for assembly of the Complex III-Complex IV supercomplex, and for assembly of Cox13p and Rcf2p into cytochrome c oxidase; similar to Rcf2p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; required for growth under hypoxic conditions; member of the hypoxia induced gene family; C. elegans and human orthologs are functional in yeast Gene:RCK1(YGL158W)|FD-Score:-3.2|P-value:6.97E-4||SGD DESC:Protein kinase involved in the response to oxidative stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; RCK1 has a paralog, RCK2, that arose from the whole genome duplication Gene:RGT1(YKL038W)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; RGT1 has a paralog, EDS1, that arose from the whole genome duplication Gene:RHO4(YKR055W)|FD-Score:-3.36|P-value:3.90E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely to be involved in the establishment of cell polarity Gene:RPL19A(YBR084C-A)|FD-Score:4.34|P-value:7.19E-6||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPL22A(YLR061W)|FD-Score:-3.26|P-value:5.59E-4||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPL35B(YDL136W)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RPL40A(YIL148W)|FD-Score:-4.19|P-value:1.39E-5||SGD DESC:Ubiquitin-ribosomal 60S subunit protein L40A fusion protein; cleaved to yield ubiquitin and ribosomal protein L40A; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40A has a paralog, RPL40B, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:RPL6A(YML073C)|FD-Score:-5.2|P-value:9.96E-8||SGD DESC:Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS16B(YDL083C)|FD-Score:4.3|P-value:8.64E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16B has a paralog, RPS16A, that arose from the whole genome duplication Gene:RPS21B(YJL136C)|FD-Score:-3.25|P-value:5.86E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication Gene:RPS22A(YJL190C)|FD-Score:4.05|P-value:2.52E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication Gene:RRT1(YBL048W_d)|FD-Score:3.86|P-value:5.73E-5||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:RSM25(YIL093C)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RTG1(YOL067C)|FD-Score:4.96|P-value:3.51E-7||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:RTT107(YHR154W)|FD-Score:6.88|P-value:3.06E-12||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SEC72(YLR292C)|FD-Score:4.33|P-value:7.32E-6||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SHY1(YGR112W)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Mitochondrial inner membrane protein required for assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes; similar to human SURF1 involved in Leigh Syndrome Gene:SIT4(YDL047W)|FD-Score:4.39|P-value:5.65E-6||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:SNX3(YOR357C)|FD-Score:-3.1|P-value:9.54E-4||SGD DESC:Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p Gene:SPC72(YAL047C)|FD-Score:3.24|P-value:6.01E-4||SGD DESC:Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization Gene:SRL1(YOR247W)|FD-Score:5.02|P-value:2.55E-7||SGD DESC:Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication Gene:SSD1(YDR293C)|FD-Score:4.09|P-value:2.15E-5||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STM1(YLR150W)|FD-Score:3.15|P-value:8.21E-4||SGD DESC:Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery Gene:STP3(YLR375W)|FD-Score:3.81|P-value:6.86E-5||SGD DESC:Zinc-finger protein of unknown function, possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids Gene:SUT1(YGL162W)|FD-Score:6.73|P-value:8.39E-12||SGD DESC:Transcription factor of the Zn2Cys6 family involved in sterol uptake; involved in induction of hypoxic gene expression; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SWR1(YDR334W)|FD-Score:5.23|P-value:8.41E-8||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TAT2(YOL020W)|FD-Score:5.07|P-value:2.01E-7||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TIP41(YPR040W)|FD-Score:4.02|P-value:2.92E-5||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:TLG2(YOL018C)|FD-Score:3.19|P-value:7.05E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TPO1(YLL028W)|FD-Score:-4.37|P-value:6.13E-6||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane Gene:TRK1(YJL129C)|FD-Score:5.6|P-value:1.07E-8||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:22.6|P-value:2.11E-113||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:10.9|P-value:6.50E-28||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UBP9(YER098W)|FD-Score:4.31|P-value:8.29E-6||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication Gene:UPS3(YDR185C)|FD-Score:4.77|P-value:9.21E-7||SGD DESC:Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations Gene:VMA9(YCL005W-A)|FD-Score:3.9|P-value:4.74E-5||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:VPH1(YOR270C)|FD-Score:-3.44|P-value:2.91E-4||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:VPS33(YLR396C)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:ATP-binding protein that is a subunit of the HOPS complex and of the CORVET tethering complex; essential for protein sorting, vesicle docking and fusion at the vacuole Gene:YBR259W(YBR259W_p)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:Protein of unknown function; YBR259W is not an essential gene; forms cytoplasmic foci upon DNA replication stress Gene:YCL002C(YCL002C_p)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YDL012C(YDL012C)|FD-Score:-5.92|P-value:1.59E-9||SGD DESC:Tail-anchored plasma membrane protein with a conserved CYSTM module; possibly involved in response to stress; may contribute to non-homologous end-joining (NHEJ) based on ydl012c htz1 double null phenotype; YDL012C has a paralog, YBR016W, that arose from the whole genome duplication Gene:YDR008C(YDR008C_d)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR182W-A(YDR182W-A_p)|FD-Score:4.32|P-value:7.87E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR444W(YDR444W_p)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Putative hydrolase acting on ester bonds Gene:YEL057C(YEL057C_p)|FD-Score:4.86|P-value:5.87E-7||SGD DESC:Protein of unknown function involved in telomere maintenance; target of UME6 regulation Gene:YER076C(YER076C_p)|FD-Score:4.63|P-value:1.85E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization Gene:YGL159W(YGL159W_p)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Putative protein of unknown function; deletion mutant has no detectable phenotype Gene:YHR049C-A(YHR049C-A_d)|FD-Score:-3.71|P-value:1.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR175W-A(YHR175W-A_p)|FD-Score:-4.24|P-value:1.14E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YIL067C(YIL067C_p)|FD-Score:-3.29|P-value:4.94E-4||SGD DESC:Uncharacterized protein of unknown function Gene:YJL213W(YJL213W)|FD-Score:-3.18|P-value:7.44E-4||SGD DESC:Protein of unknown function that may interact with ribosomes; periodically expressed during the yeast metabolic cycle; phosphorylated in vitro by the mitotic exit network (MEN) kinase complex, Dbf2p/Mob1p Gene:YKL169C(YKL169C_d)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YKU70(YMR284W)|FD-Score:-4.28|P-value:9.51E-6||SGD DESC:Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair Gene:YLR152C(YLR152C_p)|FD-Score:-3.22|P-value:6.51E-4||SGD DESC:Putative protein of unknown function; YLR152C is not an essential gene Gene:YLR358C(YLR358C_p)|FD-Score:4.03|P-value:2.84E-5||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps ORF RSC2/YLR357W Gene:YMR086C-A(YMR086C-A_d)|FD-Score:3.12|P-value:8.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR247W-A(YMR247W-A_p)|FD-Score:-4.08|P-value:2.30E-5||SGD DESC:Putative protein of unknown function Gene:YMR320W(YMR320W_d)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL203C(YNL203C_d)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR300W(YOR300W_d)|FD-Score:-4.04|P-value:2.71E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps with verified gene BUD7/YOR299W; mutation affects bipolar budding and bud site selection, though phenotype could be due to the mutation's effects on BUD7 Gene:YOR342C(YOR342C_p)|FD-Score:3.97|P-value:3.66E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOR342C has a paralog, YAL037W, that arose from the whole genome duplication Gene:YSR3(YKR053C)|FD-Score:3.56|P-value:1.89E-4||SGD DESC:Dihydrosphingosine 1-phosphate phosphatase; membrane protein involved in sphingolipid metabolism; YSR3 has a paralog, LCB3, that arose from the whole genome duplication Gene:ADP1(YCR011C)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Putative ATP-dependent permease of the ABC transporter family of proteins Gene:AIM43(YPL099C)|FD-Score:-3.26|P-value:5.62E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:AIP1(YMR092C)|FD-Score:-3.69|P-value:1.14E-4||SGD DESC:Actin cortical patch component; interacts with the actin depolymerizing factor cofilin; required to restrict cofilin localization to cortical patches; contains WD repeats; protein increases in abundance and relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:APL6(YGR261C)|FD-Score:-3.27|P-value:5.46E-4||SGD DESC:Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools Gene:ARP6(YLR085C)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:ATG33(YLR356W)|FD-Score:4.33|P-value:7.34E-6||SGD DESC:Mitochondrial mitophagy-specific protein; required primarily for mitophagy induced at the post-log phase; not required for other types of selective autophagy or macroautophagy; conserved within fungi, but not in higher eukaryotes Gene:ATP7(YKL016C)|FD-Score:3.83|P-value:6.35E-5||SGD DESC:Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:AVT4(YNL101W)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BDS1(YOL164W)|FD-Score:3.98|P-value:3.46E-5||SGD DESC:Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources Gene:CBT1(YKL208W)|FD-Score:-3.53|P-value:2.07E-4||SGD DESC:Protein involved in 5' end processing of mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p Gene:CCS1(YMR038C)|FD-Score:7.28|P-value:1.67E-13||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CDC55(YGL190C)|FD-Score:3.88|P-value:5.14E-5||SGD DESC:Non-essential regulatory subunit B of protein phosphatase 2A (PP2A); localization to the cytoplasm requires Zds1p and Zds2p and promotes mitotic entry; localization to the nucleus prevents mitotic exit; required for correct nuclear division and chromosome segregation in meiosis; maintains nucleolar sequestration of Cdc14p during early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion Gene:CDC73(YLR418C)|FD-Score:4.55|P-value:2.64E-6||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CGI121(YML036W)|FD-Score:5.38|P-value:3.71E-8||SGD DESC:Component of the EKC/KEOPS complex with Bud32p, Kae1p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription; Cgi121p is dispensable for tRNA modification Gene:CHS3(YBR023C)|FD-Score:3.4|P-value:3.31E-4||SGD DESC:Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan Gene:CHS7(YHR142W)|FD-Score:3.78|P-value:7.80E-5||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:CIN1(YOR349W)|FD-Score:7.94|P-value:1.03E-15||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:CLA4(YNL298W)|FD-Score:6.01|P-value:9.49E-10||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CLB6(YGR109C)|FD-Score:-4|P-value:3.14E-5||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 Gene:CRG1(YHR209W)|FD-Score:3.75|P-value:8.86E-5||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:CSF1(YLR087C)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CSM1(YCR086W)|FD-Score:4.49|P-value:3.48E-6||SGD DESC:Nucleolar protein that forms a complex with Lrs4p and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:CTL1(YMR180C)|FD-Score:-3.32|P-value:4.54E-4||SGD DESC:RNA 5'-triphosphatase, localizes to both the nucleus and cytoplasm Gene:CTP1(YBR291C)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Mitochondrial inner membrane citrate transporter, member of the mitochondrial carrier family Gene:CTR1(YPR124W)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CUE1(YMR264W)|FD-Score:-3.18|P-value:7.26E-4||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:CUE5(YOR042W)|FD-Score:4.26|P-value:1.00E-5||SGD DESC:Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DDI1(YER143W)|FD-Score:4.39|P-value:5.54E-6||SGD DESC:DNA damage-inducible v-SNARE binding protein with a role in suppression of protein secretion; may play a role in S-phase checkpoint control; has ubiquitin-associated (UBA), ubiquitin-like (UBL), and retroviral-like proteinase (RVP) domains Gene:DSS4(YPR017C)|FD-Score:4.97|P-value:3.43E-7||SGD DESC:Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol Gene:ECM18(YDR125C)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication Gene:FAR3(YMR052W)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:FPR4(YLR449W)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase) (proline isomerase) localized to the nucleus; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones Gene:FYV7(YLR068W)|FD-Score:-3.15|P-value:8.20E-4||SGD DESC:Essential protein required for maturation of 18S rRNA; required for survival upon exposure to K1 killer toxin Gene:GND1(YHR183W)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication Gene:GND2(YGR256W)|FD-Score:-4.02|P-value:2.86E-5||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone; GND2 has a paralog, GND1, that arose from the whole genome duplication Gene:HTZ1(YOL012C)|FD-Score:3.15|P-value:8.04E-4||SGD DESC:Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin Gene:ITC1(YGL133W)|FD-Score:-3.27|P-value:5.43E-4||SGD DESC:Subunit of the ATP-dependent Isw2p-Itc1p chromatin remodeling complex, required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex Gene:JHD2(YJR119C)|FD-Score:-3.34|P-value:4.14E-4||SGD DESC:JmjC domain family histone demethylase specific for H3-K4 (histone H3 Lys4); removes methyl groups specifically added by Set1p methyltransferase; protein levels regulated by Not4p (E3 ubiquitin ligase) polyubiquitin-mediated degradation Gene:KRE2(YDR483W)|FD-Score:4.45|P-value:4.31E-6||SGD DESC:Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication Gene:LAA1(YJL207C)|FD-Score:-3.34|P-value:4.13E-4||SGD DESC:AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene Gene:LAG2(YOL025W)|FD-Score:3.15|P-value:8.16E-4||SGD DESC:Protein that negatively regulates the SCF E3-ubiquitin ligase by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1 Gene:LTE1(YAL024C)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MAD1(YGL086W)|FD-Score:-3.7|P-value:1.10E-4||SGD DESC:Coiled-coil protein involved in the spindle-assembly checkpoint; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon spindle assembly checkpoint arrest; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of the activity of the anaphase promoting complex; forms a complex with Mad2p Gene:MDG1(YNL173C)|FD-Score:4.01|P-value:3.06E-5||SGD DESC:Plasma membrane protein involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations Gene:MET22(YOL064C)|FD-Score:-3.11|P-value:9.36E-4||SGD DESC:Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway Gene:MID2(YLR332W)|FD-Score:4|P-value:3.12E-5||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:MMR1(YLR190W)|FD-Score:-3.91|P-value:4.64E-5||SGD DESC:Phosphorylated protein of the mitochondrial outer membrane, localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p Gene:MNE1(YOR350C)|FD-Score:4.39|P-value:5.67E-6||SGD DESC:Mitochondrial matrix protein involved in splicing Group I aI5-beta intron from COX1 mRNA Gene:MNN10(YDR245W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family Gene:MOS1(YCL057C-A)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Mitochondrial protein essential for proper inner membrane organization; conserved component of the mitochondrial inner membrane organizing system (MICOS, MINOS, or MitOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture; ortholog of human MINOS1 Gene:MSA2(YKR077W)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation Gene:MUD1(YBR119W)|FD-Score:3.75|P-value:8.78E-5||SGD DESC:U1 snRNP A protein, homolog of human U1-A; involved in nuclear mRNA splicing Gene:MXR2(YCL033C)|FD-Score:5.37|P-value:3.95E-8||SGD DESC:Methionine-R-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR1; involved in the regulation of lifespan Gene:NBP2(YDR162C)|FD-Score:4.59|P-value:2.20E-6||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NKP2(YLR315W)|FD-Score:4.41|P-value:5.27E-6||SGD DESC:Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2 Gene:NPL3(YDR432W)|FD-Score:4.07|P-value:2.35E-5||SGD DESC:RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; has a role in repressing translation initiation by binding eIF4G; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm; protein abundance increases in response to DNA replication stress Gene:OM14(YBR230C)|FD-Score:-3.84|P-value:6.07E-5||SGD DESC:Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron Gene:OSH6(YKR003W)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Member of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion Gene:PAC2(YER007W)|FD-Score:5.6|P-value:1.06E-8||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PET122(YER153C)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet494p; located in the mitochondrial inner membrane Gene:PET309(YLR067C)|FD-Score:3.1|P-value:9.78E-4||SGD DESC:Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains seven pentatricopeptide repeats (PPRs) Gene:PHM8(YER037W)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Lysophosphatidic acid (LPA) phosphatase involved in LPA hydrolysis in response to phosphate starvation; phosphatase activity is soluble and Mg2+ dependent; expression is induced by low phosphate levels and by inactivation of Pho85p Gene:PPT2(YPL148C)|FD-Score:-3.59|P-value:1.64E-4||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PRO2(YOR323C)|FD-Score:-3.97|P-value:3.59E-5||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RCF1(YML030W)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Cytochrome c oxidase subunit; required for assembly of the Complex III-Complex IV supercomplex, and for assembly of Cox13p and Rcf2p into cytochrome c oxidase; similar to Rcf2p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; required for growth under hypoxic conditions; member of the hypoxia induced gene family; C. elegans and human orthologs are functional in yeast Gene:RCK1(YGL158W)|FD-Score:-3.2|P-value:6.97E-4||SGD DESC:Protein kinase involved in the response to oxidative stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; RCK1 has a paralog, RCK2, that arose from the whole genome duplication Gene:RGT1(YKL038W)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; RGT1 has a paralog, EDS1, that arose from the whole genome duplication Gene:RHO4(YKR055W)|FD-Score:-3.36|P-value:3.90E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely to be involved in the establishment of cell polarity Gene:RPL19A(YBR084C-A)|FD-Score:4.34|P-value:7.19E-6||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPL22A(YLR061W)|FD-Score:-3.26|P-value:5.59E-4||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPL35B(YDL136W)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RPL40A(YIL148W)|FD-Score:-4.19|P-value:1.39E-5||SGD DESC:Ubiquitin-ribosomal 60S subunit protein L40A fusion protein; cleaved to yield ubiquitin and ribosomal protein L40A; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40A has a paralog, RPL40B, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:RPL6A(YML073C)|FD-Score:-5.2|P-value:9.96E-8||SGD DESC:Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS16B(YDL083C)|FD-Score:4.3|P-value:8.64E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16B has a paralog, RPS16A, that arose from the whole genome duplication Gene:RPS21B(YJL136C)|FD-Score:-3.25|P-value:5.86E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication Gene:RPS22A(YJL190C)|FD-Score:4.05|P-value:2.52E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication Gene:RRT1(YBL048W_d)|FD-Score:3.86|P-value:5.73E-5||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:RSM25(YIL093C)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RTG1(YOL067C)|FD-Score:4.96|P-value:3.51E-7||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:RTT107(YHR154W)|FD-Score:6.88|P-value:3.06E-12||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SEC72(YLR292C)|FD-Score:4.33|P-value:7.32E-6||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SHY1(YGR112W)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Mitochondrial inner membrane protein required for assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes; similar to human SURF1 involved in Leigh Syndrome Gene:SIT4(YDL047W)|FD-Score:4.39|P-value:5.65E-6||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:SNX3(YOR357C)|FD-Score:-3.1|P-value:9.54E-4||SGD DESC:Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p Gene:SPC72(YAL047C)|FD-Score:3.24|P-value:6.01E-4||SGD DESC:Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization Gene:SRL1(YOR247W)|FD-Score:5.02|P-value:2.55E-7||SGD DESC:Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication Gene:SSD1(YDR293C)|FD-Score:4.09|P-value:2.15E-5||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STM1(YLR150W)|FD-Score:3.15|P-value:8.21E-4||SGD DESC:Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery Gene:STP3(YLR375W)|FD-Score:3.81|P-value:6.86E-5||SGD DESC:Zinc-finger protein of unknown function, possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids Gene:SUT1(YGL162W)|FD-Score:6.73|P-value:8.39E-12||SGD DESC:Transcription factor of the Zn2Cys6 family involved in sterol uptake; involved in induction of hypoxic gene expression; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SWR1(YDR334W)|FD-Score:5.23|P-value:8.41E-8||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TAT2(YOL020W)|FD-Score:5.07|P-value:2.01E-7||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TIP41(YPR040W)|FD-Score:4.02|P-value:2.92E-5||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:TLG2(YOL018C)|FD-Score:3.19|P-value:7.05E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TPO1(YLL028W)|FD-Score:-4.37|P-value:6.13E-6||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane Gene:TRK1(YJL129C)|FD-Score:5.6|P-value:1.07E-8||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:22.6|P-value:2.11E-113||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:10.9|P-value:6.50E-28||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UBP9(YER098W)|FD-Score:4.31|P-value:8.29E-6||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication Gene:UPS3(YDR185C)|FD-Score:4.77|P-value:9.21E-7||SGD DESC:Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations Gene:VMA9(YCL005W-A)|FD-Score:3.9|P-value:4.74E-5||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:VPH1(YOR270C)|FD-Score:-3.44|P-value:2.91E-4||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:VPS33(YLR396C)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:ATP-binding protein that is a subunit of the HOPS complex and of the CORVET tethering complex; essential for protein sorting, vesicle docking and fusion at the vacuole Gene:YBR259W(YBR259W_p)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:Protein of unknown function; YBR259W is not an essential gene; forms cytoplasmic foci upon DNA replication stress Gene:YCL002C(YCL002C_p)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YDL012C(YDL012C)|FD-Score:-5.92|P-value:1.59E-9||SGD DESC:Tail-anchored plasma membrane protein with a conserved CYSTM module; possibly involved in response to stress; may contribute to non-homologous end-joining (NHEJ) based on ydl012c htz1 double null phenotype; YDL012C has a paralog, YBR016W, that arose from the whole genome duplication Gene:YDR008C(YDR008C_d)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR182W-A(YDR182W-A_p)|FD-Score:4.32|P-value:7.87E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR444W(YDR444W_p)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Putative hydrolase acting on ester bonds Gene:YEL057C(YEL057C_p)|FD-Score:4.86|P-value:5.87E-7||SGD DESC:Protein of unknown function involved in telomere maintenance; target of UME6 regulation Gene:YER076C(YER076C_p)|FD-Score:4.63|P-value:1.85E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization Gene:YGL159W(YGL159W_p)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Putative protein of unknown function; deletion mutant has no detectable phenotype Gene:YHR049C-A(YHR049C-A_d)|FD-Score:-3.71|P-value:1.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR175W-A(YHR175W-A_p)|FD-Score:-4.24|P-value:1.14E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YIL067C(YIL067C_p)|FD-Score:-3.29|P-value:4.94E-4||SGD DESC:Uncharacterized protein of unknown function Gene:YJL213W(YJL213W)|FD-Score:-3.18|P-value:7.44E-4||SGD DESC:Protein of unknown function that may interact with ribosomes; periodically expressed during the yeast metabolic cycle; phosphorylated in vitro by the mitotic exit network (MEN) kinase complex, Dbf2p/Mob1p Gene:YKL169C(YKL169C_d)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YKU70(YMR284W)|FD-Score:-4.28|P-value:9.51E-6||SGD DESC:Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair Gene:YLR152C(YLR152C_p)|FD-Score:-3.22|P-value:6.51E-4||SGD DESC:Putative protein of unknown function; YLR152C is not an essential gene Gene:YLR358C(YLR358C_p)|FD-Score:4.03|P-value:2.84E-5||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps ORF RSC2/YLR357W Gene:YMR086C-A(YMR086C-A_d)|FD-Score:3.12|P-value:8.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR247W-A(YMR247W-A_p)|FD-Score:-4.08|P-value:2.30E-5||SGD DESC:Putative protein of unknown function Gene:YMR320W(YMR320W_d)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL203C(YNL203C_d)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR300W(YOR300W_d)|FD-Score:-4.04|P-value:2.71E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps with verified gene BUD7/YOR299W; mutation affects bipolar budding and bud site selection, though phenotype could be due to the mutation's effects on BUD7 Gene:YOR342C(YOR342C_p)|FD-Score:3.97|P-value:3.66E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOR342C has a paralog, YAL037W, that arose from the whole genome duplication Gene:YSR3(YKR053C)|FD-Score:3.56|P-value:1.89E-4||SGD DESC:Dihydrosphingosine 1-phosphate phosphatase; membrane protein involved in sphingolipid metabolism; YSR3 has a paralog, LCB3, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR272W9.777.31E-232.12YTM1Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats
YOR207C7.669.61E-150.64RET1Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
YHR062C7.021.11E-121.14RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YOR260W5.882.09E-90.18GCD1Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YPL153C5.696.22E-90.40RAD53Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication
YDR064W5.305.82E-80.45RPS13Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15
YPR142C_d4.856.16E-70.06YPR142C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing
YIL083C4.798.18E-70.02CAB2Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity)
YOR210W4.779.24E-70.42RPB10RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III
YJL195C_d4.356.89E-60.13YJL195C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6
YMR128W4.211.26E-50.09ECM16Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis
YJL111W4.131.85E-50.06CCT7Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance
YGL055W4.062.44E-50.04OLE1Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
YBR265W4.022.92E-50.65TSC103-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family
YLR033W3.373.73E-40.14RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A22.602.11E-113TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YML124C10.906.50E-28TUB3Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication
YOR349W7.941.03E-15CIN1Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl
YMR038C7.281.67E-13CCS1Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress
YHR154W6.883.06E-12RTT107Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress
YGL162W6.738.39E-12SUT1Transcription factor of the Zn2Cys6 family involved in sterol uptake; involved in induction of hypoxic gene expression; relocalizes from nucleus to cytoplasm upon DNA replication stress
YNL298W6.019.49E-10CLA4Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p
YER007W5.601.06E-8PAC2Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl
YJL129C5.601.07E-8TRK1Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication
YML036W5.383.71E-8CGI121Component of the EKC/KEOPS complex with Bud32p, Kae1p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription; Cgi121p is dispensable for tRNA modification
YCL033C5.373.95E-8MXR2Methionine-R-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR1; involved in the regulation of lifespan
YDR334W5.238.41E-8SWR1Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A
YOL020W5.072.01E-7TAT2High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance
YOR247W5.022.55E-7SRL1Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication
YPR017C4.973.43E-7DSS4Guanine nucleotide dissociation stimulator for Sec4p, functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol

GO enrichment analysis for SGTC_2952
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.3824.09E-204SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.136364
0.3791.43E-200SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.711538
0.3631.98E-183SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0571429TSC3-RPN4
0.3632.29E-183SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.197368TSC3-RPN4
0.3555.80E-174SGTC_2103551-0445 56.8 μMChemDiv (Drug-like library)28651800.164557
0.3551.58E-174SGTC_32829108498 15.5 μMChembridge (Drug-like library)164596450.666667
0.3483.93E-168SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.0857143TSC3-RPN4
0.3456.03E-165SGTC_23236148992 148.3 μMChembridge (Fragment library)28882200.316667
0.3381.95E-157SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.125TSC3-RPN4
0.3372.68E-156SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.0714286TSC3-RPN4
0.3359.42E-155SGTC_1643st009866 20.2 μMTimTec (Natural product derivative library)31562730.097561
0.3161.31E-136SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.150685TSC3-RPN4
0.3111.56E-132SGTC_5891227-0070 34.1 μMChemDiv (Drug-like library)28413840.130435
0.3052.22E-127SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.108108TSC3-RPN4
0.3002.33E-123SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.144928TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_295690761962.52 μM0.79166716423868Chembridge (Drug-like library)331.194442.54314
SGTC_2962908231113.18 μM0.79166716459453Chembridge (Drug-like library)290.357482.15714
SGTC_2958907725416.72 μM0.7616457351Chembridge (Drug-like library)292.33031.19815
SGTC_296090793895.97 μM0.7516457355Chembridge (Drug-like library)310.775962.36514
SGTC_296390825079 μM0.74509816459611Chembridge (Drug-like library)306.356881.54715
SGTC_294790726397.68 μM0.73584916488146Chembridge (Drug-like library)314.7398432.08415tubulin folding & SWR complex
SGTC_2957907680011.52 μM0.7254916459615Chembridge (Drug-like library)290.357482.18614
SGTC_294990725422.23 μM0.71698116457352Chembridge (Drug-like library)354.399682.77415TSC3-RPN4
SGTC_2959907915511 μM0.71698116189457Chembridge (Drug-like library)334.366981.41816
SGTC_331791384958.75 μM0.71153817020139Chembridge (Drug-like library)298.2852461.62516