9076196

N-(3,5-dichlorophenyl)-2-(4-ethyl-2-oxomorpholin-3-yl)acetamide

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2956
Screen concentration 2.5 μM
Source Chembridge (Drug-like library)
PubChem CID 16423868
SMILES CCN1CCOC(=O)C1CC(=O)NC2=CC(=CC(=C2)Cl)Cl
Standardized SMILES CCN1CCOC(=O)C1CC(=O)Nc2cc(Cl)cc(Cl)c2
Molecular weight 331.1944
ALogP 2.54
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.87
% growth inhibition (Hom. pool) 6.9


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 16423868
Download HIP data (tab-delimited text)  (excel)
Gene:CDC31(YOR257W)|FD-Score:3.84|P-value:6.20E-5|Clearance:0.34||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:INN1(YNL152W)|FD-Score:3.09|P-value:9.98E-4|Clearance:0.01||SGD DESC:Essential protein that associates with contractile actomyosin ring; required for ingression of the plasma membrane into the bud neck during cytokinesis; C2 domain, a membrane targeting module, is required for function; activates chitin synthase activity of Chs2p during cytokinesis Gene:MAK5(YBR142W)|FD-Score:3.16|P-value:7.78E-4|Clearance:0.07||SGD DESC:Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits Gene:MCD1(YDL003W)|FD-Score:3.32|P-value:4.42E-4|Clearance:0.09||SGD DESC:Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase Gene:NTR2(YKR022C)|FD-Score:3.24|P-value:6.07E-4|Clearance:0.02||SGD DESC:Essential protein that forms a dimer with Ntr1p; also forms a trimer, with Ntr2p and the DExD/H-box RNA helicase Prp43p, that is involved in spliceosome disassembly Gene:PRE7(YBL041W)|FD-Score:4.67|P-value:1.54E-6|Clearance:0.83||SGD DESC:Beta 6 subunit of the 20S proteasome Gene:RPF2(YKR081C)|FD-Score:3.36|P-value:3.86E-4|Clearance:0.04||SGD DESC:Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles Gene:SRP72(YPL210C)|FD-Score:3.22|P-value:6.52E-4|Clearance:0.05||SGD DESC:Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane Gene:TAF6(YGL112C)|FD-Score:5.37|P-value:3.98E-8|Clearance:0.83||SGD DESC:Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4 Gene:TSC10(YBR265W)|FD-Score:3.5|P-value:2.31E-4|Clearance:0.14||SGD DESC:3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family Gene:UTP20(YBL004W)|FD-Score:5.06|P-value:2.10E-7|Clearance:0.83||SGD DESC:Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA Gene:CDC31(YOR257W)|FD-Score:3.84|P-value:6.20E-5|Clearance:0.34||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:INN1(YNL152W)|FD-Score:3.09|P-value:9.98E-4|Clearance:0.01||SGD DESC:Essential protein that associates with contractile actomyosin ring; required for ingression of the plasma membrane into the bud neck during cytokinesis; C2 domain, a membrane targeting module, is required for function; activates chitin synthase activity of Chs2p during cytokinesis Gene:MAK5(YBR142W)|FD-Score:3.16|P-value:7.78E-4|Clearance:0.07||SGD DESC:Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits Gene:MCD1(YDL003W)|FD-Score:3.32|P-value:4.42E-4|Clearance:0.09||SGD DESC:Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase Gene:NTR2(YKR022C)|FD-Score:3.24|P-value:6.07E-4|Clearance:0.02||SGD DESC:Essential protein that forms a dimer with Ntr1p; also forms a trimer, with Ntr2p and the DExD/H-box RNA helicase Prp43p, that is involved in spliceosome disassembly Gene:PRE7(YBL041W)|FD-Score:4.67|P-value:1.54E-6|Clearance:0.83||SGD DESC:Beta 6 subunit of the 20S proteasome Gene:RPF2(YKR081C)|FD-Score:3.36|P-value:3.86E-4|Clearance:0.04||SGD DESC:Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles Gene:SRP72(YPL210C)|FD-Score:3.22|P-value:6.52E-4|Clearance:0.05||SGD DESC:Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane Gene:TAF6(YGL112C)|FD-Score:5.37|P-value:3.98E-8|Clearance:0.83||SGD DESC:Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4 Gene:TSC10(YBR265W)|FD-Score:3.5|P-value:2.31E-4|Clearance:0.14||SGD DESC:3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family Gene:UTP20(YBL004W)|FD-Score:5.06|P-value:2.10E-7|Clearance:0.83||SGD DESC:Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 16423868
Download HOP data (tab-delimited text)  (excel)
Gene:AFT1(YGL071W)|FD-Score:3.87|P-value:5.48E-5||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AIM41(YOR215C)|FD-Score:3.14|P-value:8.42E-4||SGD DESC:Putative protein of unknown function; the authentic protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:APM2(YHL019C)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport Gene:AQY2(YLL052C)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains Gene:ATG18(YFR021W)|FD-Score:-3.34|P-value:4.22E-4||SGD DESC:Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood Gene:ATP23(YNR020C)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Putative metalloprotease of the mitochondrial inner membrane, required for processing of Atp6p; has an additional role in assembly of the F0 sector of the F1F0 ATP synthase complex; substrate of the Mia40p-Erv1p disulfide relay system, and folding is assisted by Mia40p Gene:CCW14(YLR390W-A)|FD-Score:-3.11|P-value:9.42E-4||SGD DESC:Covalently linked cell wall glycoprotein, present in the inner layer of the cell wall Gene:COG6(YNL041C)|FD-Score:4.08|P-value:2.24E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSN9(YDR179C)|FD-Score:7.65|P-value:1.03E-14||SGD DESC:Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling Gene:CYS3(YAL012W)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DBR1(YKL149C)|FD-Score:3.97|P-value:3.54E-5||SGD DESC:RNA lariat debranching enzyme, involved in intron turnover; required for efficient Ty1 transposition Gene:DPL1(YDR294C)|FD-Score:-3.23|P-value:6.08E-4||SGD DESC:Dihydrosphingosine phosphate lyase, regulates intracellular levels of sphingolipid long-chain base phosphates (LCBPs), degrades phosphorylated long chain bases, prefers C16 dihydrosphingosine-l-phosphate as a substrate Gene:EGT2(YNL327W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Glycosylphosphatidylinositol (GPI)-anchored cell wall endoglucanase required for proper cell separation after cytokinesis, expression is activated by Swi5p and tightly regulated in a cell cycle-dependent manner Gene:EMC6(YLL014W)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm F33D4.7/EMC-6, fly CG11781, human TMEM93 Gene:GEF1(YJR040W)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism Gene:GLT1(YDL171C)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source Gene:GPG1(YGL121C)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Proposed gamma subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p; involved in regulation of pseudohyphal growth; requires Gpb1p or Gpb2p to interact with Gpa2p; overproduction causes prion curing Gene:HEL2(YDR266C)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; computational analysis suggests a role as a transcription factor Gene:HIR1(YBL008W)|FD-Score:3.39|P-value:3.53E-4||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores Gene:HIR3(YJR140C)|FD-Score:-6.12|P-value:4.76E-10||SGD DESC:Subunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein Gene:HRD1(YOL013C)|FD-Score:-3.24|P-value:6.04E-4||SGD DESC:Ubiquitin-protein ligase; required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon Gene:IMG1(YCR046C)|FD-Score:-4.58|P-value:2.37E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome Gene:IMP2'(YIL154C)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:INM2(YDR287W)|FD-Score:4.06|P-value:2.48E-5||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol; enzymatic activity requires magnesium ions and is inhibited by lithium and sodium ions; inm1 inm2 double mutant lacks inositol auxotrophy Gene:IPK1(YDR315C)|FD-Score:4.68|P-value:1.46E-6||SGD DESC:Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable Gene:IPT1(YDR072C)|FD-Score:-3.96|P-value:3.72E-5||SGD DESC:Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin Gene:KDX1(YKL161C)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p Gene:LCB3(YJL134W)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Long-chain base-1-phosphate phosphatase; specific for dihydrosphingosine-1-phosphate, regulates ceramide and long-chain base phosphates levels, involved in incorporation of exogenous long chain bases in sphingolipids; LCB3 has a paralog, YSR3, that arose from the whole genome duplication Gene:LRO1(YNR008W)|FD-Score:3.81|P-value:6.82E-5||SGD DESC:Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase Gene:MAL33(YBR297W)|FD-Score:-3.18|P-value:7.27E-4||SGD DESC:MAL-activator protein, part of complex locus MAL3; nonfunctional in genomic reference strain S288C Gene:MCR1(YKL150W)|FD-Score:4.36|P-value:6.61E-6||SGD DESC:Mitochondrial NADH-cytochrome b5 reductase, involved in ergosterol biosynthesis Gene:MDG1(YNL173C)|FD-Score:3.19|P-value:7.07E-4||SGD DESC:Plasma membrane protein involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations Gene:MSB1(YOR188W)|FD-Score:3.89|P-value:5.06E-5||SGD DESC:Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:OSH2(YDL019C)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:OST6(YML019W)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p Gene:OSW2(YLR054C)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Protein of unknown function proposed to be involved in the assembly of the spore wall Gene:PAN3(YKL025C)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PDR3(YBL005W)|FD-Score:3.56|P-value:1.82E-4||SGD DESC:Transcriptional activator of the pleiotropic drug resistance network; regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting sites known as PDREs (PDR responsive elements); post-translationally up-regulated in cells lacking a functional mitochondrial genome; PDR3 has a paralog, PDR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:PEX27(YOR193W)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Peripheral peroxisomal membrane protein involved in controlling peroxisome size and number, interacts with homologous protein Pex25p Gene:PML1(YLR016C)|FD-Score:-4.11|P-value:1.94E-5||SGD DESC:Subunit of the RES complex, which is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p Gene:PRM5(YIL117C)|FD-Score:-3.32|P-value:4.56E-4||SGD DESC:Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling Gene:PRM7(YDL039C)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Pheromone-regulated protein, predicted to have one transmembrane segment; promoter contains Gcn4p binding elements Gene:RBS1(YDL189W)|FD-Score:-3.36|P-value:3.83E-4||SGD DESC:Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain Gene:RMD9(YGL107C)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes Gene:RTK1(YDL025C)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress Gene:SCS3(YGL126W)|FD-Score:5.02|P-value:2.64E-7||SGD DESC:Protein required for inositol prototrophy; required for normal ER membrane biosynthesis; ortholog of the FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; disputed role in the synthesis of inositol phospholipids from inositol Gene:SFA1(YDL168W)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress Gene:SLM3(YDL033C)|FD-Score:3.33|P-value:4.38E-4||SGD DESC:tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) Gene:SML1(YML058W)|FD-Score:-3.97|P-value:3.55E-5||SGD DESC:Ribonucleotide reductase inhibitor involved in regulating dNTP production; regulated by Mec1p and Rad53p during DNA damage and S phase Gene:SNT1(YCR033W)|FD-Score:3.9|P-value:4.86E-5||SGD DESC:Subunit of the Set3C deacetylase complex that interacts directly with the Set3C subunit, Sif2p; putative DNA-binding protein; mutant has increased aneuploidy tolerance Gene:SPE1(YKL184W)|FD-Score:4.83|P-value:6.80E-7||SGD DESC:Ornithine decarboxylase, catalyzes the first step in polyamine biosynthesis; degraded in a proteasome-dependent manner in the presence of excess polyamines; deletion decreases lifespan, and increases necrotic cell death and ROS generation Gene:SPT4(YGR063C)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:TCB2(YNL087W)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains 3 calcium and lipid binding domains; mRNA is targeted to the bud Gene:TDA6(YPR157W_p)|FD-Score:3.21|P-value:6.58E-4||SGD DESC:Putative protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; null mutant is sensitive to expression of the top1-T722A allele Gene:THP3(YPR045C)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Protein that forms a complex with Csn12p that is recruited to transcribed genes and may have a role in transcription elongation; possibly involved in splicing based on pre-mRNA accumulation defect for many intron-containing genes Gene:TKL1(YPR074C)|FD-Score:3.84|P-value:6.22E-5||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:TLG2(YOL018C)|FD-Score:4.34|P-value:7.07E-6||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TOS2(YGR221C)|FD-Score:3.99|P-value:3.37E-5||SGD DESC:Protein involved in localization of Cdc24p to the site of bud growth; may act as a membrane anchor; localizes to the bud neck and bud tip; potentially phosphorylated by Cdc28p Gene:TPC1(YGR096W)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Mitochondrial membrane transporter that mediates uptake of the essential cofactor thiamine pyrophosphate (ThPP) into mitochondria; expression appears to be regulated by carbon source; member of the mitochondrial carrier family Gene:TRP2(YER090W)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UBX6(YJL048C)|FD-Score:-3.52|P-value:2.16E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p, transcription is repressed when cells are grown in media containing inositol and choline; UBX6 has a paralog, UBX7, that arose from the whole genome duplication Gene:ULI1(YFR026C)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:Putative protein of unknown function involved in and induced by the endoplasmic reticulum unfolded protein response Gene:VBA3(YCL069W)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:YBL029W(YBL029W_p)|FD-Score:5.73|P-value:4.98E-9||SGD DESC:Non-essential protein of unknown function Gene:YBL044W(YBL044W_p)|FD-Score:3.83|P-value:6.40E-5||SGD DESC:Putative protein of unknown function; YBL044W is not an essential protein Gene:YBL100W-C(YBL100W-C_p)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR056W-A(YBR056W-A_p)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the dubious ORF YBR056C-B Gene:YBR224W(YBR224W_d)|FD-Score:3.79|P-value:7.63E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TDP1 Gene:YDL144C(YDL144C_p)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YDL162C(YDL162C_d)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps ENT1/YDL161W, a verified gene involved in endocytosis and actin cortical patch assembly Gene:YDR455C(YDR455C_d)|FD-Score:-4.18|P-value:1.44E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YDR491C(YDR491C_d)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL138C(YGL138C_p)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Putative protein of unknown function; has no significant sequence similarity to any known protein Gene:YGR035W-A(YGR035W-A_p)|FD-Score:-4.14|P-value:1.71E-5||SGD DESC:Putative protein of unknown function Gene:YHR050W-A(YHR050W-A_p)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Protein of unknown function; identified by expression profiling and mass spectrometry Gene:YHR125W(YHR125W_d)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL022W(YJL022W_d)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130 Gene:YJR120W(YJR120W)|FD-Score:8.72|P-value:1.44E-18||SGD DESC:Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p Gene:YKL107W(YKL107W_p)|FD-Score:-4.41|P-value:5.14E-6||SGD DESC:Putative short-chain dehydrogenase/reductase; proposed to be a palmitoylated membrane protein Gene:YLL032C(YLL032C)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene Gene:YMR099C(YMR099C)|FD-Score:5.23|P-value:8.61E-8||SGD DESC:Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:YNL170W(YNL170W_d)|FD-Score:3.94|P-value:4.04E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL303W(YNL303W_d)|FD-Score:-5.4|P-value:3.24E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPK2(YMR104C)|FD-Score:-3.2|P-value:6.81E-4||SGD DESC:Protein kinase with similarity to serine/threonine protein kinase Ypk1p; functionally redundant with YPK1 at the genetic level; participates in a signaling pathway required for optimal cell wall integrity; homolog of mammalian kinase SGK; YPK2 has a paralog, YPK1, that arose from the whole genome duplication Gene:YPS1(YLR120C)|FD-Score:4.86|P-value:5.75E-7||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:YSP1(YHR155W)|FD-Score:-4.54|P-value:2.87E-6||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication Gene:YUH1(YJR099W)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Ubiquitin C-terminal hydrolase that cleaves ubiquitin-protein fusions to generate monomeric ubiquitin; hydrolyzes the peptide bond at the C-terminus of ubiquitin; also the major processing enzyme for the ubiquitin-like protein Rub1p Gene:AFT1(YGL071W)|FD-Score:3.87|P-value:5.48E-5||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AIM41(YOR215C)|FD-Score:3.14|P-value:8.42E-4||SGD DESC:Putative protein of unknown function; the authentic protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:APM2(YHL019C)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport Gene:AQY2(YLL052C)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains Gene:ATG18(YFR021W)|FD-Score:-3.34|P-value:4.22E-4||SGD DESC:Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood Gene:ATP23(YNR020C)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Putative metalloprotease of the mitochondrial inner membrane, required for processing of Atp6p; has an additional role in assembly of the F0 sector of the F1F0 ATP synthase complex; substrate of the Mia40p-Erv1p disulfide relay system, and folding is assisted by Mia40p Gene:CCW14(YLR390W-A)|FD-Score:-3.11|P-value:9.42E-4||SGD DESC:Covalently linked cell wall glycoprotein, present in the inner layer of the cell wall Gene:COG6(YNL041C)|FD-Score:4.08|P-value:2.24E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSN9(YDR179C)|FD-Score:7.65|P-value:1.03E-14||SGD DESC:Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling Gene:CYS3(YAL012W)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DBR1(YKL149C)|FD-Score:3.97|P-value:3.54E-5||SGD DESC:RNA lariat debranching enzyme, involved in intron turnover; required for efficient Ty1 transposition Gene:DPL1(YDR294C)|FD-Score:-3.23|P-value:6.08E-4||SGD DESC:Dihydrosphingosine phosphate lyase, regulates intracellular levels of sphingolipid long-chain base phosphates (LCBPs), degrades phosphorylated long chain bases, prefers C16 dihydrosphingosine-l-phosphate as a substrate Gene:EGT2(YNL327W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Glycosylphosphatidylinositol (GPI)-anchored cell wall endoglucanase required for proper cell separation after cytokinesis, expression is activated by Swi5p and tightly regulated in a cell cycle-dependent manner Gene:EMC6(YLL014W)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm F33D4.7/EMC-6, fly CG11781, human TMEM93 Gene:GEF1(YJR040W)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism Gene:GLT1(YDL171C)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source Gene:GPG1(YGL121C)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Proposed gamma subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p; involved in regulation of pseudohyphal growth; requires Gpb1p or Gpb2p to interact with Gpa2p; overproduction causes prion curing Gene:HEL2(YDR266C)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; computational analysis suggests a role as a transcription factor Gene:HIR1(YBL008W)|FD-Score:3.39|P-value:3.53E-4||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores Gene:HIR3(YJR140C)|FD-Score:-6.12|P-value:4.76E-10||SGD DESC:Subunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein Gene:HRD1(YOL013C)|FD-Score:-3.24|P-value:6.04E-4||SGD DESC:Ubiquitin-protein ligase; required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon Gene:IMG1(YCR046C)|FD-Score:-4.58|P-value:2.37E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome Gene:IMP2'(YIL154C)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:INM2(YDR287W)|FD-Score:4.06|P-value:2.48E-5||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol; enzymatic activity requires magnesium ions and is inhibited by lithium and sodium ions; inm1 inm2 double mutant lacks inositol auxotrophy Gene:IPK1(YDR315C)|FD-Score:4.68|P-value:1.46E-6||SGD DESC:Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable Gene:IPT1(YDR072C)|FD-Score:-3.96|P-value:3.72E-5||SGD DESC:Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin Gene:KDX1(YKL161C)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p Gene:LCB3(YJL134W)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Long-chain base-1-phosphate phosphatase; specific for dihydrosphingosine-1-phosphate, regulates ceramide and long-chain base phosphates levels, involved in incorporation of exogenous long chain bases in sphingolipids; LCB3 has a paralog, YSR3, that arose from the whole genome duplication Gene:LRO1(YNR008W)|FD-Score:3.81|P-value:6.82E-5||SGD DESC:Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase Gene:MAL33(YBR297W)|FD-Score:-3.18|P-value:7.27E-4||SGD DESC:MAL-activator protein, part of complex locus MAL3; nonfunctional in genomic reference strain S288C Gene:MCR1(YKL150W)|FD-Score:4.36|P-value:6.61E-6||SGD DESC:Mitochondrial NADH-cytochrome b5 reductase, involved in ergosterol biosynthesis Gene:MDG1(YNL173C)|FD-Score:3.19|P-value:7.07E-4||SGD DESC:Plasma membrane protein involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations Gene:MSB1(YOR188W)|FD-Score:3.89|P-value:5.06E-5||SGD DESC:Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:OSH2(YDL019C)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:OST6(YML019W)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p Gene:OSW2(YLR054C)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Protein of unknown function proposed to be involved in the assembly of the spore wall Gene:PAN3(YKL025C)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PDR3(YBL005W)|FD-Score:3.56|P-value:1.82E-4||SGD DESC:Transcriptional activator of the pleiotropic drug resistance network; regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting sites known as PDREs (PDR responsive elements); post-translationally up-regulated in cells lacking a functional mitochondrial genome; PDR3 has a paralog, PDR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:PEX27(YOR193W)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Peripheral peroxisomal membrane protein involved in controlling peroxisome size and number, interacts with homologous protein Pex25p Gene:PML1(YLR016C)|FD-Score:-4.11|P-value:1.94E-5||SGD DESC:Subunit of the RES complex, which is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p Gene:PRM5(YIL117C)|FD-Score:-3.32|P-value:4.56E-4||SGD DESC:Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling Gene:PRM7(YDL039C)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Pheromone-regulated protein, predicted to have one transmembrane segment; promoter contains Gcn4p binding elements Gene:RBS1(YDL189W)|FD-Score:-3.36|P-value:3.83E-4||SGD DESC:Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain Gene:RMD9(YGL107C)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes Gene:RTK1(YDL025C)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress Gene:SCS3(YGL126W)|FD-Score:5.02|P-value:2.64E-7||SGD DESC:Protein required for inositol prototrophy; required for normal ER membrane biosynthesis; ortholog of the FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; disputed role in the synthesis of inositol phospholipids from inositol Gene:SFA1(YDL168W)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress Gene:SLM3(YDL033C)|FD-Score:3.33|P-value:4.38E-4||SGD DESC:tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) Gene:SML1(YML058W)|FD-Score:-3.97|P-value:3.55E-5||SGD DESC:Ribonucleotide reductase inhibitor involved in regulating dNTP production; regulated by Mec1p and Rad53p during DNA damage and S phase Gene:SNT1(YCR033W)|FD-Score:3.9|P-value:4.86E-5||SGD DESC:Subunit of the Set3C deacetylase complex that interacts directly with the Set3C subunit, Sif2p; putative DNA-binding protein; mutant has increased aneuploidy tolerance Gene:SPE1(YKL184W)|FD-Score:4.83|P-value:6.80E-7||SGD DESC:Ornithine decarboxylase, catalyzes the first step in polyamine biosynthesis; degraded in a proteasome-dependent manner in the presence of excess polyamines; deletion decreases lifespan, and increases necrotic cell death and ROS generation Gene:SPT4(YGR063C)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:TCB2(YNL087W)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains 3 calcium and lipid binding domains; mRNA is targeted to the bud Gene:TDA6(YPR157W_p)|FD-Score:3.21|P-value:6.58E-4||SGD DESC:Putative protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; null mutant is sensitive to expression of the top1-T722A allele Gene:THP3(YPR045C)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Protein that forms a complex with Csn12p that is recruited to transcribed genes and may have a role in transcription elongation; possibly involved in splicing based on pre-mRNA accumulation defect for many intron-containing genes Gene:TKL1(YPR074C)|FD-Score:3.84|P-value:6.22E-5||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:TLG2(YOL018C)|FD-Score:4.34|P-value:7.07E-6||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TOS2(YGR221C)|FD-Score:3.99|P-value:3.37E-5||SGD DESC:Protein involved in localization of Cdc24p to the site of bud growth; may act as a membrane anchor; localizes to the bud neck and bud tip; potentially phosphorylated by Cdc28p Gene:TPC1(YGR096W)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Mitochondrial membrane transporter that mediates uptake of the essential cofactor thiamine pyrophosphate (ThPP) into mitochondria; expression appears to be regulated by carbon source; member of the mitochondrial carrier family Gene:TRP2(YER090W)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UBX6(YJL048C)|FD-Score:-3.52|P-value:2.16E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p, transcription is repressed when cells are grown in media containing inositol and choline; UBX6 has a paralog, UBX7, that arose from the whole genome duplication Gene:ULI1(YFR026C)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:Putative protein of unknown function involved in and induced by the endoplasmic reticulum unfolded protein response Gene:VBA3(YCL069W)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:YBL029W(YBL029W_p)|FD-Score:5.73|P-value:4.98E-9||SGD DESC:Non-essential protein of unknown function Gene:YBL044W(YBL044W_p)|FD-Score:3.83|P-value:6.40E-5||SGD DESC:Putative protein of unknown function; YBL044W is not an essential protein Gene:YBL100W-C(YBL100W-C_p)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR056W-A(YBR056W-A_p)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the dubious ORF YBR056C-B Gene:YBR224W(YBR224W_d)|FD-Score:3.79|P-value:7.63E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TDP1 Gene:YDL144C(YDL144C_p)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YDL162C(YDL162C_d)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps ENT1/YDL161W, a verified gene involved in endocytosis and actin cortical patch assembly Gene:YDR455C(YDR455C_d)|FD-Score:-4.18|P-value:1.44E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YDR491C(YDR491C_d)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL138C(YGL138C_p)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Putative protein of unknown function; has no significant sequence similarity to any known protein Gene:YGR035W-A(YGR035W-A_p)|FD-Score:-4.14|P-value:1.71E-5||SGD DESC:Putative protein of unknown function Gene:YHR050W-A(YHR050W-A_p)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Protein of unknown function; identified by expression profiling and mass spectrometry Gene:YHR125W(YHR125W_d)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL022W(YJL022W_d)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130 Gene:YJR120W(YJR120W)|FD-Score:8.72|P-value:1.44E-18||SGD DESC:Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p Gene:YKL107W(YKL107W_p)|FD-Score:-4.41|P-value:5.14E-6||SGD DESC:Putative short-chain dehydrogenase/reductase; proposed to be a palmitoylated membrane protein Gene:YLL032C(YLL032C)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene Gene:YMR099C(YMR099C)|FD-Score:5.23|P-value:8.61E-8||SGD DESC:Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:YNL170W(YNL170W_d)|FD-Score:3.94|P-value:4.04E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL303W(YNL303W_d)|FD-Score:-5.4|P-value:3.24E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPK2(YMR104C)|FD-Score:-3.2|P-value:6.81E-4||SGD DESC:Protein kinase with similarity to serine/threonine protein kinase Ypk1p; functionally redundant with YPK1 at the genetic level; participates in a signaling pathway required for optimal cell wall integrity; homolog of mammalian kinase SGK; YPK2 has a paralog, YPK1, that arose from the whole genome duplication Gene:YPS1(YLR120C)|FD-Score:4.86|P-value:5.75E-7||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:YSP1(YHR155W)|FD-Score:-4.54|P-value:2.87E-6||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication Gene:YUH1(YJR099W)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Ubiquitin C-terminal hydrolase that cleaves ubiquitin-protein fusions to generate monomeric ubiquitin; hydrolyzes the peptide bond at the C-terminus of ubiquitin; also the major processing enzyme for the ubiquitin-like protein Rub1p

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL112C5.373.98E-80.83TAF6Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4
YBL004W5.062.10E-70.83UTP20Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA
YBL041W4.671.54E-60.83PRE7Beta 6 subunit of the 20S proteasome
YOR257W3.846.20E-50.34CDC31Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation
YBR265W3.502.31E-40.14TSC103-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family
YKR081C3.363.86E-40.04RPF2Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles
YDL003W3.324.42E-40.09MCD1Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase
YKR022C3.246.07E-40.02NTR2Essential protein that forms a dimer with Ntr1p; also forms a trimer, with Ntr2p and the DExD/H-box RNA helicase Prp43p, that is involved in spliceosome disassembly
YPL210C3.226.52E-40.05SRP72Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane
YBR142W3.167.78E-40.07MAK5Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits
YNL152W3.099.98E-40.01INN1Essential protein that associates with contractile actomyosin ring; required for ingression of the plasma membrane into the bud neck during cytokinesis; C2 domain, a membrane targeting module, is required for function; activates chitin synthase activity of Chs2p during cytokinesis
YDR376W3.080.001040.09ARH1Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability
YGL102C_d2.980.001420.01YGL102C_dDubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential RPL28 gene encoding a large subunit ribosomal protein
YKL195W2.980.001450.06MIA40Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p
YOR103C2.910.001790.08OST2Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJR120W8.721.44E-18YJR120WProtein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
YDR179C7.651.03E-14CSN9Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling
YBL029W_p5.734.98E-9YBL029W_pNon-essential protein of unknown function
YMR099C5.238.61E-8YMR099CGlucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS
YGL126W5.022.64E-7SCS3Protein required for inositol prototrophy; required for normal ER membrane biosynthesis; ortholog of the FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; disputed role in the synthesis of inositol phospholipids from inositol
YLR120C4.865.75E-7YPS1Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor
YKL184W4.836.80E-7SPE1Ornithine decarboxylase, catalyzes the first step in polyamine biosynthesis; degraded in a proteasome-dependent manner in the presence of excess polyamines; deletion decreases lifespan, and increases necrotic cell death and ROS generation
YDR315C4.681.46E-6IPK1Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable
YKL150W4.366.61E-6MCR1Mitochondrial NADH-cytochrome b5 reductase, involved in ergosterol biosynthesis
YOL018C4.347.07E-6TLG2Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16)
YGL138C_p4.191.40E-5YGL138C_pPutative protein of unknown function; has no significant sequence similarity to any known protein
YNL041C4.082.24E-5COG6Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YDR287W4.062.48E-5INM2Inositol monophosphatase, involved in biosynthesis of inositol; enzymatic activity requires magnesium ions and is inhibited by lithium and sodium ions; inm1 inm2 double mutant lacks inositol auxotrophy
YGR221C3.993.37E-5TOS2Protein involved in localization of Cdc24p to the site of bud growth; may act as a membrane anchor; localizes to the bud neck and bud tip; potentially phosphorylated by Cdc28p
YKL149C3.973.54E-5DBR1RNA lariat debranching enzyme, involved in intron turnover; required for efficient Ty1 transposition

GO enrichment analysis for SGTC_2956
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3231.03E-143SGTC_29619083290 32.5 μMChembridge (Drug-like library)177414900.181818
0.1132.40E-18SGTC_21005377183 190.0 μMChembridge (Fragment library)28442020.16417960S ribosome export
0.0961.81E-13SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.101695
0.0921.44E-12SGTC_15110906-3109 28.5 μMChemDiv (Drug-like library)28821800.0649351excess fatty acid
0.0896.82E-12SGTC_11594092-0538 61.2 μMChemDiv (Drug-like library)7173010.179104
0.0881.05E-11SGTC_29629082311 13.2 μMChembridge (Drug-like library)164594530.686275
0.0864.28E-11SGTC_30059076496 71.4 μMChembridge (Drug-like library)213684100.176471
0.0864.50E-11SGTC_1953st076800 12.1 μMTimTec (Natural product derivative library)2975910.0441176
0.0855.00E-11SGTC_15666,7-dimethoxy-4-ethylcoumarin 85.4 μMTimTec (Pure natural product library)6887610.138462
0.0807.74E-10SGTC_8230417-1665 178.0 μMChemDiv (Drug-like library)34298560.0795455
0.0791.05E-9SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.08
0.0791.24E-9SGTC_450kpi-0036 14.9 μMChemDiv (Drug-like library)27950210.0923077excess fatty acid
0.0782.01E-9SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.114286
0.0759.89E-9SGTC_21685727979 200.0 μMChembridge (Fragment library)6952100.0909091
0.0741.01E-8SGTC_25772',4'-dihydroxy-4-methoxychalcone 15.3 μMMicrosource (Natural product library)57112230.0972222

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2952907373113.78 μM0.79166716457353Chembridge (Drug-like library)296.749381.87814tubulin folding & SWR complex
SGTC_296090793895.97 μM0.71698116457355Chembridge (Drug-like library)310.775962.36514
SGTC_294790726397.68 μM0.70370416488146Chembridge (Drug-like library)314.7398432.08415tubulin folding & SWR complex
SGTC_2957907680011.52 μM0.69230816459615Chembridge (Drug-like library)290.357482.18614
SGTC_2962908231113.18 μM0.68627516459453Chembridge (Drug-like library)290.357482.15714
SGTC_331791384958.75 μM0.67924517020139Chembridge (Drug-like library)298.2852461.62516
SGTC_3282910849815.46 μM0.66666716459645Chembridge (Drug-like library)280.2947831.4215
SGTC_2958907725416.72 μM0.66037716457351Chembridge (Drug-like library)292.33031.19815
SGTC_296390825079 μM0.64814816459611Chembridge (Drug-like library)306.356881.54715
SGTC_294990725422.23 μM0.62516457352Chembridge (Drug-like library)354.399682.77415TSC3-RPN4