9077254

2-(4-ethyl-2-oxomorpholin-3-yl)-N-(4-methoxyphenyl)acetamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2958
Screen concentration 16.7 μM
Source Chembridge (Drug-like library)
PubChem CID 16457351
SMILES CCN1CCOC(=O)C1CC(=O)NC2=CC=C(C=C2)OC
Standardized SMILES CCN1CCOC(=O)C1CC(=O)Nc2ccc(OC)cc2
Molecular weight 292.3303
ALogP 1.2
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.32
% growth inhibition (Hom. pool) 6.9


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 16457351
Download HIP data (tab-delimited text)  (excel)
Gene:ARH1(YDR376W)|FD-Score:3.57|P-value:1.78E-4|Clearance:0.04||SGD DESC:Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability Gene:BUR6(YER159C)|FD-Score:-3.33|P-value:4.32E-4|Clearance:0||SGD DESC:Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha Gene:DAM1(YGR113W)|FD-Score:3.17|P-value:7.59E-4|Clearance:0.08||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments Gene:DBP2(YNL112W)|FD-Score:3.81|P-value:6.91E-5|Clearance:0.21||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:ERB1(YMR049C)|FD-Score:-3.69|P-value:1.10E-4|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1 Gene:ERG8(YMR220W)|FD-Score:5.03|P-value:2.47E-7|Clearance:0.55||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:HRR25(YPL204W)|FD-Score:-3.92|P-value:4.45E-5|Clearance:0||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:MTR2(YKL186C)|FD-Score:-3.79|P-value:7.66E-5|Clearance:0||SGD DESC:mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA Gene:NFS1(YCL017C)|FD-Score:4.24|P-value:1.12E-5|Clearance:0.17||SGD DESC:Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria Gene:NOG1(YPL093W)|FD-Score:3.53|P-value:2.05E-4|Clearance:0.03||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:PRP6(YBR055C)|FD-Score:-3.43|P-value:3.04E-4|Clearance:0||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RLP24(YLR009W)|FD-Score:3.35|P-value:4.01E-4|Clearance:0.14||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPB3(YIL021W)|FD-Score:3.1|P-value:9.81E-4|Clearance:0.09||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPL33A(YPL143W)|FD-Score:-3.96|P-value:3.80E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication Gene:RPO31(YOR116C)|FD-Score:-3.28|P-value:5.24E-4|Clearance:0||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:SEC39(YLR440C)|FD-Score:-3.12|P-value:9.08E-4|Clearance:0||SGD DESC:Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope Gene:SEN1(YLR430W)|FD-Score:-5.92|P-value:1.62E-9|Clearance:0||SGD DESC:Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS Gene:SPB4(YFL002C)|FD-Score:3.5|P-value:2.33E-4|Clearance:0.12||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:SPC110(YDR356W)|FD-Score:5.85|P-value:2.52E-9|Clearance:0.82||SGD DESC:Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner Gene:SSS1(YDR086C)|FD-Score:4.07|P-value:2.40E-5|Clearance:0.25||SGD DESC:Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p Gene:TFC6(YDR362C)|FD-Score:4.48|P-value:3.77E-6|Clearance:0.24||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:TOM22(YNL131W)|FD-Score:3.38|P-value:3.58E-4|Clearance:0.03||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:YDL196W(YDL196W_d)|FD-Score:3.21|P-value:6.53E-4|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YGR115C(YGR115C_d)|FD-Score:3.6|P-value:1.58E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YLR230W(YLR230W_d)|FD-Score:-4.19|P-value:1.40E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity Gene:ARH1(YDR376W)|FD-Score:3.57|P-value:1.78E-4|Clearance:0.04||SGD DESC:Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability Gene:BUR6(YER159C)|FD-Score:-3.33|P-value:4.32E-4|Clearance:0||SGD DESC:Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha Gene:DAM1(YGR113W)|FD-Score:3.17|P-value:7.59E-4|Clearance:0.08||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments Gene:DBP2(YNL112W)|FD-Score:3.81|P-value:6.91E-5|Clearance:0.21||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:ERB1(YMR049C)|FD-Score:-3.69|P-value:1.10E-4|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1 Gene:ERG8(YMR220W)|FD-Score:5.03|P-value:2.47E-7|Clearance:0.55||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:HRR25(YPL204W)|FD-Score:-3.92|P-value:4.45E-5|Clearance:0||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:MTR2(YKL186C)|FD-Score:-3.79|P-value:7.66E-5|Clearance:0||SGD DESC:mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA Gene:NFS1(YCL017C)|FD-Score:4.24|P-value:1.12E-5|Clearance:0.17||SGD DESC:Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria Gene:NOG1(YPL093W)|FD-Score:3.53|P-value:2.05E-4|Clearance:0.03||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:PRP6(YBR055C)|FD-Score:-3.43|P-value:3.04E-4|Clearance:0||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RLP24(YLR009W)|FD-Score:3.35|P-value:4.01E-4|Clearance:0.14||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPB3(YIL021W)|FD-Score:3.1|P-value:9.81E-4|Clearance:0.09||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPL33A(YPL143W)|FD-Score:-3.96|P-value:3.80E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication Gene:RPO31(YOR116C)|FD-Score:-3.28|P-value:5.24E-4|Clearance:0||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:SEC39(YLR440C)|FD-Score:-3.12|P-value:9.08E-4|Clearance:0||SGD DESC:Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope Gene:SEN1(YLR430W)|FD-Score:-5.92|P-value:1.62E-9|Clearance:0||SGD DESC:Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS Gene:SPB4(YFL002C)|FD-Score:3.5|P-value:2.33E-4|Clearance:0.12||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:SPC110(YDR356W)|FD-Score:5.85|P-value:2.52E-9|Clearance:0.82||SGD DESC:Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner Gene:SSS1(YDR086C)|FD-Score:4.07|P-value:2.40E-5|Clearance:0.25||SGD DESC:Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p Gene:TFC6(YDR362C)|FD-Score:4.48|P-value:3.77E-6|Clearance:0.24||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:TOM22(YNL131W)|FD-Score:3.38|P-value:3.58E-4|Clearance:0.03||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:YDL196W(YDL196W_d)|FD-Score:3.21|P-value:6.53E-4|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YGR115C(YGR115C_d)|FD-Score:3.6|P-value:1.58E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YLR230W(YLR230W_d)|FD-Score:-4.19|P-value:1.40E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 16457351
Download HOP data (tab-delimited text)  (excel)
Gene:AAP1(YHR047C)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication Gene:ADE5,7(YGL234W)|FD-Score:4.88|P-value:5.22E-7||SGD DESC:Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities Gene:AHA1(YDR214W)|FD-Score:6.24|P-value:2.20E-10||SGD DESC:Co-chaperone that binds to Hsp82p and activates its ATPase activity; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress Gene:ALK1(YGL021W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK1 has a paralog, ALK2, that arose from the whole genome duplication; similar to mammalian haspins Gene:APT2(YDR441C)|FD-Score:-3.34|P-value:4.14E-4||SGD DESC:Potential adenine phosphoribosyltransferase; encodes a protein with similarity to adenine phosphoribosyltransferase, but artificially expressed protein exhibits no enzymatic activity; APT2 has a paralog, APT1, that arose from the whole genome duplication Gene:BLI1(YKL061W_p)|FD-Score:-4.55|P-value:2.66E-6||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome Gene:BSD2(YBR290W)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Heavy metal ion homeostasis protein, facilitates trafficking of Smf1p and Smf2p metal transporters to the vacuole where they are degraded, controls metal ion transport, prevents metal hyperaccumulation, functions in copper detoxification Gene:CCC2(YDR270W)|FD-Score:4.23|P-value:1.18E-5||SGD DESC:Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism Gene:CCW12(YLR110C)|FD-Score:-3.59|P-value:1.67E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CCZ1(YBR131W)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway Gene:CHZ1(YER030W)|FD-Score:-3.31|P-value:4.63E-4||SGD DESC:Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif; protein abundance increases in response to DNA replication stress Gene:CLC1(YGR167W)|FD-Score:3.16|P-value:7.93E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:CLN2(YPL256C)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) Gene:COG8(YML071C)|FD-Score:-4.24|P-value:1.13E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CYS3(YAL012W)|FD-Score:-3.59|P-value:1.65E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:EAF7(YNL136W)|FD-Score:4.15|P-value:1.63E-5||SGD DESC:Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A Gene:EGT2(YNL327W)|FD-Score:4.57|P-value:2.45E-6||SGD DESC:Glycosylphosphatidylinositol (GPI)-anchored cell wall endoglucanase required for proper cell separation after cytokinesis, expression is activated by Swi5p and tightly regulated in a cell cycle-dependent manner Gene:EMI2(YDR516C)|FD-Score:6.81|P-value:4.73E-12||SGD DESC:Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:EMP24(YGL200C)|FD-Score:3.72|P-value:9.84E-5||SGD DESC:Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles Gene:ERP1(YAR002C-A)|FD-Score:6.25|P-value:2.05E-10||SGD DESC:Member of the p24 family involved in ER to Golgi transport; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication Gene:ERV14(YGL054C)|FD-Score:-3.51|P-value:2.26E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FHN1(YGR131W)|FD-Score:-3.33|P-value:4.39E-4||SGD DESC:Protein of unknown function; induced by ketoconazole; promoter region contains sterol regulatory element motif, which has been identified as a Upc2p-binding site; overexpression complements function of Nce102p in NCE102 deletion strain Gene:FKH1(YIL131C)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Forkhead family transcription factor; has a minor role in the expression of G2/M phase genes; negatively regulates transcriptional elongation; positive role in chromatin silencing at HML and HMR; binds to the recombination enhancer near HML, and regulates donor preference during mating-type switching by positioning HML near the MAT locus through FHA-domain mediated interactions with proteins near the DSB; FKH1 has a paralog, FKH2, that arose from the whole genome duplication Gene:GET3(YDL100C)|FD-Score:-3.26|P-value:5.50E-4||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:HFM1(YGL251C)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity Gene:HOM2(YDR158W)|FD-Score:6.24|P-value:2.21E-10||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:HPR1(YDR138W)|FD-Score:-3.55|P-value:1.96E-4||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:HRD1(YOL013C)|FD-Score:3.85|P-value:5.96E-5||SGD DESC:Ubiquitin-protein ligase; required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon Gene:ICS3(YJL077C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Protein of unknown function; may play a role in vacuolar sorting, null mutants are hypersensitive to sortin2 Gene:IDP1(YDL066W)|FD-Score:3.18|P-value:7.33E-4||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:IES1(YFL013C)|FD-Score:4.15|P-value:1.68E-5||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:IRS4(YKR019C)|FD-Score:-3.16|P-value:7.94E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:JIP4(YDR475C)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence Gene:KTI11(YBL071W-A)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs Gene:LDB16(YCL005W)|FD-Score:-3.42|P-value:3.13E-4||SGD DESC:Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria Gene:MGA1(YGR249W)|FD-Score:4.59|P-value:2.25E-6||SGD DESC:Protein similar to heat shock transcription factor; multicopy suppressor of pseudohyphal growth defects of ammonium permease mutants Gene:MKC7(YDR144C)|FD-Score:4.6|P-value:2.14E-6||SGD DESC:GPI-anchored aspartyl protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; shares functions with Yap3p and Kex2p Gene:MYO5(YMR109W)|FD-Score:4.42|P-value:4.92E-6||SGD DESC:One of two type I myosins; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization Gene:OMS1(YDR316W)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:OPI9(YLR338W_d)|FD-Score:-3.26|P-value:5.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PDE2(YOR360C)|FD-Score:4.54|P-value:2.85E-6||SGD DESC:High-affinity cyclic AMP phosphodiesterase, component of the cAMP-dependent protein kinase signaling system, protects the cell from extracellular cAMP, contains readthrough motif surrounding termination codon Gene:PET20(YPL159C)|FD-Score:-3.72|P-value:9.82E-5||SGD DESC:Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome Gene:PGM3(YMR278W)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential Gene:PRO2(YOR323C)|FD-Score:5.77|P-value:4.04E-9||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PSY3(YLR376C)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C Gene:PTC5(YOR090C)|FD-Score:3.91|P-value:4.67E-5||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) involved in regulation of pyruvate dehydrogenase activity by dephosphorylating the serine 133 of the Pda1p subunit; localizes to the intermembrane space and is imported via the presequence pathway and processed by the inner membrane protease (Imp1p-Imp2p); acts in concert with kinases Pkp1p and Pkp2p and phosphatase Ptc6p Gene:RAD52(YML032C)|FD-Score:-6.81|P-value:5.01E-12||SGD DESC:Protein that stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis Gene:RAD7(YJR052W)|FD-Score:-4.25|P-value:1.07E-5||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad16p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RAS2(YNL098C)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes Gene:RCK1(YGL158W)|FD-Score:4.34|P-value:7.04E-6||SGD DESC:Protein kinase involved in the response to oxidative stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; RCK1 has a paralog, RCK2, that arose from the whole genome duplication Gene:RPL24B(YGR148C)|FD-Score:-3.54|P-value:2.00E-4||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:RPL2A(YFR031C-A)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Ribosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication Gene:RRT1(YBL048W_d)|FD-Score:3.89|P-value:5.02E-5||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:RTC5(YOR118W)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; null mutation suppresses cdc13-1 temperature sensitivity Gene:RTR1(YER139C)|FD-Score:3.94|P-value:4.00E-5||SGD DESC:CTD phosphatase; dephosphorylates S5-P in the C-terminal domain of Rpo21p; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm; RTR1 has a paralog, RTR2, that arose from the whole genome duplication Gene:RTT101(YJL047C)|FD-Score:3.14|P-value:8.35E-4||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:SAC1(YKL212W)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAC6(YDR129C)|FD-Score:-3.32|P-value:4.49E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SCS22(YBL091C-A)|FD-Score:-3.97|P-value:3.61E-5||SGD DESC:Protein involved in regulation of phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; similar to D. melanogaster inturned protein; SCS22 has a paralog, SCS2, that arose from the whole genome duplication Gene:SED1(YDR077W)|FD-Score:3.25|P-value:5.84E-4||SGD DESC:Major stress-induced structural GPI-cell wall glycoprotein in stationary-phase cells, associates with translating ribosomes, possible role in mitochondrial genome maintenance; ORF contains two distinct variable minisatellites Gene:SIW14(YNL032W)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Tyrosine phosphatase that plays a role in actin filament organization and endocytosis; localized to the cytoplasm Gene:SKT5(YBL061C)|FD-Score:6.37|P-value:9.21E-11||SGD DESC:Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication Gene:SOP4(YJL192C)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER Gene:SRP40(YKR092C)|FD-Score:3.25|P-value:5.84E-4||SGD DESC:Nucleolar, serine-rich protein with a role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140 Gene:SWC3(YAL011W)|FD-Score:3.99|P-value:3.25E-5||SGD DESC:Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae Gene:THI20(YOL055C)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Trifunctional enzyme of thiamine biosynthesis, degradation and salvage; has hydroxymethylpyrimidine (HMP) kinase, HMP-phosphate (HMP-P) kinase and thiaminase activities; member of a gene family with THI21 and THI22; HMP and HMP-P kinase activity redundant with Thi21p Gene:TLG2(YOL018C)|FD-Score:-3.23|P-value:6.23E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TPN1(YGL186C)|FD-Score:3.84|P-value:6.21E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRK1(YJL129C)|FD-Score:4.2|P-value:1.36E-5||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:TRP5(YGL026C)|FD-Score:-3.56|P-value:1.82E-4||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:UBP5(YER144C)|FD-Score:-3.5|P-value:2.32E-4||SGD DESC:Putative ubiquitin-specific protease, closest paralog of Doa4p but has no functional overlap; concentrates at the bud neck Gene:UBR1(YGR184C)|FD-Score:-3.49|P-value:2.42E-4||SGD DESC:E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) Gene:UGA2(YBR006W)|FD-Score:3.18|P-value:7.49E-4||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:UTH1(YKR042W)|FD-Score:-3.26|P-value:5.64E-4||SGD DESC:Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication Gene:VAC8(YEL013W)|FD-Score:8.75|P-value:1.11E-18||SGD DESC:Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions Gene:XRS2(YDR369C)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling Gene:YCR025C(YCR025C_d)|FD-Score:-3.5|P-value:2.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene Gene:YFR035C(YFR035C_p)|FD-Score:3.75|P-value:8.85E-5||SGD DESC:Putative protein of unknown function, deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YGL138C(YGL138C_p)|FD-Score:-3.86|P-value:5.60E-5||SGD DESC:Putative protein of unknown function; has no significant sequence similarity to any known protein Gene:YIL025C(YIL025C_d)|FD-Score:4.57|P-value:2.43E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL092W(YIL092W_p)|FD-Score:-3.88|P-value:5.28E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YJR084W(YJR084W)|FD-Score:4.06|P-value:2.47E-5||SGD DESC:Protein that forms a complex with Thp3p; may have a role in transcription elongation and/or mRNA splicing; identified as a COP9 signalosome component but phenotype and interactions suggest it may not be involved with the signalosome Gene:YKL030W(YKL030W_d)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified gene MAE1 Gene:YKL115C(YKL115C_d)|FD-Score:5.38|P-value:3.65E-8||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1 Gene:YLL059C(YLL059C_d)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR111W(YLR111W_d)|FD-Score:4.7|P-value:1.30E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR125W(YLR125W_p)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene Gene:YLR281C(YLR281C_p)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR281C is not an essential gene Gene:YLR413W(YLR413W_p)|FD-Score:-4.35|P-value:6.67E-6||SGD DESC:Putative protein of unknown function; YLR413W is not an essential gene Gene:YML007C-A(YML007C-A_p)|FD-Score:4.43|P-value:4.77E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YML119W(YML119W_p)|FD-Score:4.55|P-value:2.66E-6||SGD DESC:Putative protein of unknown function; YML119W is not an essential gene; potential Cdc28p substrate Gene:YMR090W(YMR090W_p)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Putative protein of unknown function with similarity to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability Gene:YMR099C(YMR099C)|FD-Score:3.19|P-value:7.22E-4||SGD DESC:Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:YNL022C(YNL022C_p)|FD-Score:6.18|P-value:3.18E-10||SGD DESC:Putative cytosine 5-methyltransferase, contains seven beta-strand methyltransferase motif similar to NOP2/YNL061W; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; predicted to be involved in ribosome biogenesis Gene:YNL089C(YNL089C_d)|FD-Score:3.84|P-value:6.11E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; almost completely overlaps YNL090W/RHO2 which encodes a small GTPase of the Rho/Rac subfamily of Ras-like proteins Gene:YNL235C(YNL235C_d)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YOL118C(YOL118C_d)|FD-Score:4.5|P-value:3.38E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YSC84(YHR016C)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication Gene:AAP1(YHR047C)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication Gene:ADE5,7(YGL234W)|FD-Score:4.88|P-value:5.22E-7||SGD DESC:Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities Gene:AHA1(YDR214W)|FD-Score:6.24|P-value:2.20E-10||SGD DESC:Co-chaperone that binds to Hsp82p and activates its ATPase activity; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress Gene:ALK1(YGL021W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK1 has a paralog, ALK2, that arose from the whole genome duplication; similar to mammalian haspins Gene:APT2(YDR441C)|FD-Score:-3.34|P-value:4.14E-4||SGD DESC:Potential adenine phosphoribosyltransferase; encodes a protein with similarity to adenine phosphoribosyltransferase, but artificially expressed protein exhibits no enzymatic activity; APT2 has a paralog, APT1, that arose from the whole genome duplication Gene:BLI1(YKL061W_p)|FD-Score:-4.55|P-value:2.66E-6||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome Gene:BSD2(YBR290W)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Heavy metal ion homeostasis protein, facilitates trafficking of Smf1p and Smf2p metal transporters to the vacuole where they are degraded, controls metal ion transport, prevents metal hyperaccumulation, functions in copper detoxification Gene:CCC2(YDR270W)|FD-Score:4.23|P-value:1.18E-5||SGD DESC:Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism Gene:CCW12(YLR110C)|FD-Score:-3.59|P-value:1.67E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CCZ1(YBR131W)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway Gene:CHZ1(YER030W)|FD-Score:-3.31|P-value:4.63E-4||SGD DESC:Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif; protein abundance increases in response to DNA replication stress Gene:CLC1(YGR167W)|FD-Score:3.16|P-value:7.93E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:CLN2(YPL256C)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) Gene:COG8(YML071C)|FD-Score:-4.24|P-value:1.13E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CYS3(YAL012W)|FD-Score:-3.59|P-value:1.65E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:EAF7(YNL136W)|FD-Score:4.15|P-value:1.63E-5||SGD DESC:Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A Gene:EGT2(YNL327W)|FD-Score:4.57|P-value:2.45E-6||SGD DESC:Glycosylphosphatidylinositol (GPI)-anchored cell wall endoglucanase required for proper cell separation after cytokinesis, expression is activated by Swi5p and tightly regulated in a cell cycle-dependent manner Gene:EMI2(YDR516C)|FD-Score:6.81|P-value:4.73E-12||SGD DESC:Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:EMP24(YGL200C)|FD-Score:3.72|P-value:9.84E-5||SGD DESC:Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles Gene:ERP1(YAR002C-A)|FD-Score:6.25|P-value:2.05E-10||SGD DESC:Member of the p24 family involved in ER to Golgi transport; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication Gene:ERV14(YGL054C)|FD-Score:-3.51|P-value:2.26E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FHN1(YGR131W)|FD-Score:-3.33|P-value:4.39E-4||SGD DESC:Protein of unknown function; induced by ketoconazole; promoter region contains sterol regulatory element motif, which has been identified as a Upc2p-binding site; overexpression complements function of Nce102p in NCE102 deletion strain Gene:FKH1(YIL131C)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Forkhead family transcription factor; has a minor role in the expression of G2/M phase genes; negatively regulates transcriptional elongation; positive role in chromatin silencing at HML and HMR; binds to the recombination enhancer near HML, and regulates donor preference during mating-type switching by positioning HML near the MAT locus through FHA-domain mediated interactions with proteins near the DSB; FKH1 has a paralog, FKH2, that arose from the whole genome duplication Gene:GET3(YDL100C)|FD-Score:-3.26|P-value:5.50E-4||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:HFM1(YGL251C)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity Gene:HOM2(YDR158W)|FD-Score:6.24|P-value:2.21E-10||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:HPR1(YDR138W)|FD-Score:-3.55|P-value:1.96E-4||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:HRD1(YOL013C)|FD-Score:3.85|P-value:5.96E-5||SGD DESC:Ubiquitin-protein ligase; required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon Gene:ICS3(YJL077C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Protein of unknown function; may play a role in vacuolar sorting, null mutants are hypersensitive to sortin2 Gene:IDP1(YDL066W)|FD-Score:3.18|P-value:7.33E-4||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:IES1(YFL013C)|FD-Score:4.15|P-value:1.68E-5||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:IRS4(YKR019C)|FD-Score:-3.16|P-value:7.94E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:JIP4(YDR475C)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence Gene:KTI11(YBL071W-A)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs Gene:LDB16(YCL005W)|FD-Score:-3.42|P-value:3.13E-4||SGD DESC:Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria Gene:MGA1(YGR249W)|FD-Score:4.59|P-value:2.25E-6||SGD DESC:Protein similar to heat shock transcription factor; multicopy suppressor of pseudohyphal growth defects of ammonium permease mutants Gene:MKC7(YDR144C)|FD-Score:4.6|P-value:2.14E-6||SGD DESC:GPI-anchored aspartyl protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; shares functions with Yap3p and Kex2p Gene:MYO5(YMR109W)|FD-Score:4.42|P-value:4.92E-6||SGD DESC:One of two type I myosins; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization Gene:OMS1(YDR316W)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:OPI9(YLR338W_d)|FD-Score:-3.26|P-value:5.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PDE2(YOR360C)|FD-Score:4.54|P-value:2.85E-6||SGD DESC:High-affinity cyclic AMP phosphodiesterase, component of the cAMP-dependent protein kinase signaling system, protects the cell from extracellular cAMP, contains readthrough motif surrounding termination codon Gene:PET20(YPL159C)|FD-Score:-3.72|P-value:9.82E-5||SGD DESC:Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome Gene:PGM3(YMR278W)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential Gene:PRO2(YOR323C)|FD-Score:5.77|P-value:4.04E-9||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PSY3(YLR376C)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C Gene:PTC5(YOR090C)|FD-Score:3.91|P-value:4.67E-5||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) involved in regulation of pyruvate dehydrogenase activity by dephosphorylating the serine 133 of the Pda1p subunit; localizes to the intermembrane space and is imported via the presequence pathway and processed by the inner membrane protease (Imp1p-Imp2p); acts in concert with kinases Pkp1p and Pkp2p and phosphatase Ptc6p Gene:RAD52(YML032C)|FD-Score:-6.81|P-value:5.01E-12||SGD DESC:Protein that stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis Gene:RAD7(YJR052W)|FD-Score:-4.25|P-value:1.07E-5||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad16p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RAS2(YNL098C)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes Gene:RCK1(YGL158W)|FD-Score:4.34|P-value:7.04E-6||SGD DESC:Protein kinase involved in the response to oxidative stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; RCK1 has a paralog, RCK2, that arose from the whole genome duplication Gene:RPL24B(YGR148C)|FD-Score:-3.54|P-value:2.00E-4||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:RPL2A(YFR031C-A)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Ribosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication Gene:RRT1(YBL048W_d)|FD-Score:3.89|P-value:5.02E-5||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:RTC5(YOR118W)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; null mutation suppresses cdc13-1 temperature sensitivity Gene:RTR1(YER139C)|FD-Score:3.94|P-value:4.00E-5||SGD DESC:CTD phosphatase; dephosphorylates S5-P in the C-terminal domain of Rpo21p; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm; RTR1 has a paralog, RTR2, that arose from the whole genome duplication Gene:RTT101(YJL047C)|FD-Score:3.14|P-value:8.35E-4||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:SAC1(YKL212W)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAC6(YDR129C)|FD-Score:-3.32|P-value:4.49E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SCS22(YBL091C-A)|FD-Score:-3.97|P-value:3.61E-5||SGD DESC:Protein involved in regulation of phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; similar to D. melanogaster inturned protein; SCS22 has a paralog, SCS2, that arose from the whole genome duplication Gene:SED1(YDR077W)|FD-Score:3.25|P-value:5.84E-4||SGD DESC:Major stress-induced structural GPI-cell wall glycoprotein in stationary-phase cells, associates with translating ribosomes, possible role in mitochondrial genome maintenance; ORF contains two distinct variable minisatellites Gene:SIW14(YNL032W)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Tyrosine phosphatase that plays a role in actin filament organization and endocytosis; localized to the cytoplasm Gene:SKT5(YBL061C)|FD-Score:6.37|P-value:9.21E-11||SGD DESC:Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication Gene:SOP4(YJL192C)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER Gene:SRP40(YKR092C)|FD-Score:3.25|P-value:5.84E-4||SGD DESC:Nucleolar, serine-rich protein with a role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140 Gene:SWC3(YAL011W)|FD-Score:3.99|P-value:3.25E-5||SGD DESC:Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae Gene:THI20(YOL055C)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Trifunctional enzyme of thiamine biosynthesis, degradation and salvage; has hydroxymethylpyrimidine (HMP) kinase, HMP-phosphate (HMP-P) kinase and thiaminase activities; member of a gene family with THI21 and THI22; HMP and HMP-P kinase activity redundant with Thi21p Gene:TLG2(YOL018C)|FD-Score:-3.23|P-value:6.23E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TPN1(YGL186C)|FD-Score:3.84|P-value:6.21E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRK1(YJL129C)|FD-Score:4.2|P-value:1.36E-5||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:TRP5(YGL026C)|FD-Score:-3.56|P-value:1.82E-4||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:UBP5(YER144C)|FD-Score:-3.5|P-value:2.32E-4||SGD DESC:Putative ubiquitin-specific protease, closest paralog of Doa4p but has no functional overlap; concentrates at the bud neck Gene:UBR1(YGR184C)|FD-Score:-3.49|P-value:2.42E-4||SGD DESC:E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) Gene:UGA2(YBR006W)|FD-Score:3.18|P-value:7.49E-4||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:UTH1(YKR042W)|FD-Score:-3.26|P-value:5.64E-4||SGD DESC:Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication Gene:VAC8(YEL013W)|FD-Score:8.75|P-value:1.11E-18||SGD DESC:Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions Gene:XRS2(YDR369C)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling Gene:YCR025C(YCR025C_d)|FD-Score:-3.5|P-value:2.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene Gene:YFR035C(YFR035C_p)|FD-Score:3.75|P-value:8.85E-5||SGD DESC:Putative protein of unknown function, deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YGL138C(YGL138C_p)|FD-Score:-3.86|P-value:5.60E-5||SGD DESC:Putative protein of unknown function; has no significant sequence similarity to any known protein Gene:YIL025C(YIL025C_d)|FD-Score:4.57|P-value:2.43E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL092W(YIL092W_p)|FD-Score:-3.88|P-value:5.28E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YJR084W(YJR084W)|FD-Score:4.06|P-value:2.47E-5||SGD DESC:Protein that forms a complex with Thp3p; may have a role in transcription elongation and/or mRNA splicing; identified as a COP9 signalosome component but phenotype and interactions suggest it may not be involved with the signalosome Gene:YKL030W(YKL030W_d)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified gene MAE1 Gene:YKL115C(YKL115C_d)|FD-Score:5.38|P-value:3.65E-8||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1 Gene:YLL059C(YLL059C_d)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR111W(YLR111W_d)|FD-Score:4.7|P-value:1.30E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR125W(YLR125W_p)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene Gene:YLR281C(YLR281C_p)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR281C is not an essential gene Gene:YLR413W(YLR413W_p)|FD-Score:-4.35|P-value:6.67E-6||SGD DESC:Putative protein of unknown function; YLR413W is not an essential gene Gene:YML007C-A(YML007C-A_p)|FD-Score:4.43|P-value:4.77E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YML119W(YML119W_p)|FD-Score:4.55|P-value:2.66E-6||SGD DESC:Putative protein of unknown function; YML119W is not an essential gene; potential Cdc28p substrate Gene:YMR090W(YMR090W_p)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Putative protein of unknown function with similarity to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability Gene:YMR099C(YMR099C)|FD-Score:3.19|P-value:7.22E-4||SGD DESC:Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:YNL022C(YNL022C_p)|FD-Score:6.18|P-value:3.18E-10||SGD DESC:Putative cytosine 5-methyltransferase, contains seven beta-strand methyltransferase motif similar to NOP2/YNL061W; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; predicted to be involved in ribosome biogenesis Gene:YNL089C(YNL089C_d)|FD-Score:3.84|P-value:6.11E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; almost completely overlaps YNL090W/RHO2 which encodes a small GTPase of the Rho/Rac subfamily of Ras-like proteins Gene:YNL235C(YNL235C_d)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YOL118C(YOL118C_d)|FD-Score:4.5|P-value:3.38E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YSC84(YHR016C)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR356W5.852.52E-90.82SPC110Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner
YMR220W5.032.47E-70.55ERG8Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YDR362C4.483.77E-60.24TFC6One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110
YCL017C4.241.12E-50.17NFS1Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria
YDR086C4.072.40E-50.25SSS1Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p
YNL112W3.816.91E-50.21DBP2ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites
YGR115C_d3.601.58E-40.03YGR115C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C
YDR376W3.571.78E-40.04ARH1Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability
YPL093W3.532.05E-40.03NOG1Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins
YFL002C3.502.33E-40.12SPB4Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients
YNL131W3.383.58E-40.03TOM22Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes
YLR009W3.354.01E-40.14RLP24Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
YDL196W_d3.216.53E-40.04YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YGR113W3.177.59E-40.08DAM1Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments
YIL021W3.109.81E-40.09RPB3RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YEL013W8.751.11E-18VAC8Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions
YDR516C6.814.73E-12EMI2Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
YBL061C6.379.21E-11SKT5Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication
YAR002C-A6.252.05E-10ERP1Member of the p24 family involved in ER to Golgi transport; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication
YDR214W6.242.20E-10AHA1Co-chaperone that binds to Hsp82p and activates its ATPase activity; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress
YDR158W6.242.21E-10HOM2Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
YNL022C_p6.183.18E-10YNL022C_pPutative cytosine 5-methyltransferase, contains seven beta-strand methyltransferase motif similar to NOP2/YNL061W; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; predicted to be involved in ribosome biogenesis
YOR323C5.774.04E-9PRO2Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis
YKL115C_d5.383.65E-8YKL115C_dDubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1
YGL234W4.885.22E-7ADE5,7Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities
YLR111W_d4.701.30E-6YLR111W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YDR144C4.602.14E-6MKC7GPI-anchored aspartyl protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; shares functions with Yap3p and Kex2p
YGR249W4.592.25E-6MGA1Protein similar to heat shock transcription factor; multicopy suppressor of pseudohyphal growth defects of ammonium permease mutants
YIL025C_d4.572.43E-6YIL025C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YNL327W4.572.45E-6EGT2Glycosylphosphatidylinositol (GPI)-anchored cell wall endoglucanase required for proper cell separation after cytokinesis, expression is activated by Swi5p and tightly regulated in a cell cycle-dependent manner

GO enrichment analysis for SGTC_2958
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0872.10E-11SGTC_12750867-0008 21.1 μMChemDiv (Drug-like library)34228360.150685
0.0791.52E-9SGTC_31619100388 49.5 μMChembridge (Drug-like library)252363680.1iron homeostasis
0.0731.84E-8SGTC_31759106517 49.5 μMChembridge (Drug-like library)414680730.183099iron homeostasis
0.0699.25E-8SGTC_1812st052057 4.3 μMTimTec (Natural product derivative library)36454180.0879121calcium & mitochondrial duress
0.0681.60E-7SGTC_31059121040 49.5 μMChembridge (Drug-like library)414489070.183099
0.0649.57E-7SGTC_30549094397 49.5 μMChembridge (Drug-like library)25983540.140845fatty acid desaturase (OLE1)
0.0649.74E-7SGTC_1776st049249 45.9 μMTimTec (Natural product derivative library)18223140.0823529
0.0631.36E-6SGTC_5820987-0078 156.0 μMChemDiv (Drug-like library)16257650.128205calcium & mitochondrial duress
0.0622.11E-6SGTC_1130099-0221 2.5 μMChemDiv (Drug-like library)892190.0609756
0.0612.85E-6SGTC_8093998-0021 90.0 μMChemDiv (Drug-like library)7154850.135135RNA processing & uracil transport
0.0604.10E-6SGTC_3710195-0006 7.4 μMChemDiv (Drug-like library)39285930.0769231
0.0603.45E-6SGTC_23749071832 200.0 μMChembridge (Fragment library)173816980.0909091endomembrane recycling
0.0596.19E-6SGTC_24575731572 11.7 μMMiscellaneous28653570.1375
0.0571.07E-5SGTC_228caffeine 993.1 μMMiscellaneous25190.0909091
0.0571.23E-5SGTC_21445628481 200.0 μMChembridge (Fragment library)28183990.149254ERG2

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_296390825079 μM0.8216459611Chembridge (Drug-like library)306.356881.54715
SGTC_294990725422.23 μM0.78846216457352Chembridge (Drug-like library)354.399682.77415TSC3-RPN4
SGTC_2952907373113.78 μM0.7616457353Chembridge (Drug-like library)296.749381.87814tubulin folding & SWR complex
SGTC_2962908231113.18 μM0.7616459453Chembridge (Drug-like library)290.357482.15714
SGTC_2959907915511 μM0.72222216189457Chembridge (Drug-like library)334.366981.41816
SGTC_2957907680011.52 μM0.69811316459615Chembridge (Drug-like library)290.357482.18614
SGTC_331791384958.75 μM0.68518517020139Chembridge (Drug-like library)298.2852461.62516
SGTC_296090793895.97 μM0.66071416457355Chembridge (Drug-like library)310.775962.36514
SGTC_295690761962.52 μM0.66037716423868Chembridge (Drug-like library)331.194442.54314
SGTC_294790726397.68 μM0.64912316488146Chembridge (Drug-like library)314.7398432.08415tubulin folding & SWR complex