1222-0039

(E)-3-(4-butylphenyl)but-2-enoic acid

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_296
Screen concentration 74.9 μM
Source ChemDiv (Drug-like library)
PubChem CID 5984176
SMILES CCCCC1=CC=C(C=C1)C(=CC(=O)O)C
Standardized SMILES CCCCc1ccc(cc1)C(=CC(=O)O)C
Molecular weight 218.2915
ALogP 4.23
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.52
% growth inhibition (Hom. pool) 9.43


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5984176
Download HIP data (tab-delimited text)  (excel)
Gene:ILS1(YBL076C)|FD-Score:3.2|P-value:6.98E-4|Clearance:0.07||SGD DESC:Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A Gene:KEI1(YDR367W)|FD-Score:3.79|P-value:7.39E-5|Clearance:0.32||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:RPB10(YOR210W)|FD-Score:-3.91|P-value:4.67E-5|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:SEC39(YLR440C)|FD-Score:3.12|P-value:8.96E-4|Clearance:0.05||SGD DESC:Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope Gene:SKP1(YDR328C)|FD-Score:3.45|P-value:2.79E-4|Clearance:0.11||SGD DESC:Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress Gene:TRM112(YNR046W)|FD-Score:3.47|P-value:2.59E-4|Clearance:0.02||SGD DESC:Protein involved in methylation of tRNA, rRNA, and translation factors; subunit of tRNA methyltransferase (MTase) complexes in combination with Trm9p and Trm11p; interacts with and stabilizes 18S rRNA methyltransferase Bud23p; subunit of complex with Mtq2p that methylates Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; deletion confers resistance to zymocin; relative distribution to the nucleus increases upon DNA replication stress Gene:TUB2(YFL037W)|FD-Score:3.34|P-value:4.15E-4|Clearance:0.15||SGD DESC:Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria Gene:ILS1(YBL076C)|FD-Score:3.2|P-value:6.98E-4|Clearance:0.07||SGD DESC:Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A Gene:KEI1(YDR367W)|FD-Score:3.79|P-value:7.39E-5|Clearance:0.32||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:RPB10(YOR210W)|FD-Score:-3.91|P-value:4.67E-5|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:SEC39(YLR440C)|FD-Score:3.12|P-value:8.96E-4|Clearance:0.05||SGD DESC:Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope Gene:SKP1(YDR328C)|FD-Score:3.45|P-value:2.79E-4|Clearance:0.11||SGD DESC:Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress Gene:TRM112(YNR046W)|FD-Score:3.47|P-value:2.59E-4|Clearance:0.02||SGD DESC:Protein involved in methylation of tRNA, rRNA, and translation factors; subunit of tRNA methyltransferase (MTase) complexes in combination with Trm9p and Trm11p; interacts with and stabilizes 18S rRNA methyltransferase Bud23p; subunit of complex with Mtq2p that methylates Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; deletion confers resistance to zymocin; relative distribution to the nucleus increases upon DNA replication stress Gene:TUB2(YFL037W)|FD-Score:3.34|P-value:4.15E-4|Clearance:0.15||SGD DESC:Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5984176
Download HOP data (tab-delimited text)  (excel)
Gene:ADR1(YDR216W)|FD-Score:3.21|P-value:6.60E-4||SGD DESC:Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization Gene:BIO2(YGR286C)|FD-Score:4.18|P-value:1.43E-5||SGD DESC:Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant Gene:BRP1(YGL007W_d)|FD-Score:5.14|P-value:1.36E-7||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:BSC4(YNL269W)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p Gene:BTS1(YPL069C)|FD-Score:9.96|P-value:1.18E-23||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CWH43(YCR017C)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:FLC3(YGL139W)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Putative FAD transporter, similar to Flc1p and Flc2p; localized to the ER Gene:GCN2(YDR283C)|FD-Score:8.08|P-value:3.11E-16||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GRX6(YDL010W)|FD-Score:-3.29|P-value:4.98E-4||SGD DESC:Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication Gene:HAL1(YPR005C)|FD-Score:-4.33|P-value:7.50E-6||SGD DESC:Cytoplasmic protein involved in halotolerance; decreases intracellular Na+ (via Ena1p) and increases intracellular K+ by decreasing efflux; expression repressed by Ssn6p-Tup1p and Sko1p and induced by NaCl, KCl, and sorbitol through Gcn4p Gene:HPF1(YOL155C)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Haze-protective mannoprotein that reduces the particle size of aggregated proteins in white wines Gene:JEN1(YKL217W)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Monocarboxylate/proton symporter of the plasma membrane; transport activity is dependent on the pH gradient across the membrane; mediates high-affinity uptake of carbon sources lactate, pyuvate, and acetate, and also of the micronutrient selenite, whose structure mimics that of monocarboxylates; expression and localization are tightly regulated, with transcription repression, mRNA degradation, and protein endocytosis and degradation all occurring in the presence of glucose Gene:MNT3(YIL014W)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Alpha-1,3-mannosyltransferase, adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation Gene:MTC3(YGL226W)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1 Gene:MVP1(YMR004W)|FD-Score:-3.52|P-value:2.14E-4||SGD DESC:Protein required for sorting proteins to the vacuole; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions; protein abundance increases in response to DNA replication stress Gene:MYO4(YAL029C)|FD-Score:3.14|P-value:8.42E-4||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication Gene:PAC11(YDR488C)|FD-Score:3.73|P-value:9.57E-5||SGD DESC:Dynein intermediate chain, acts in the cytoplasmic dynein pathway, forms cortical cytoplasmic microtubule capture site with Num1p; null mutant is defective in nuclear migration, essential in the absence of CIN8 Gene:PHA2(YNL316C)|FD-Score:4.37|P-value:6.22E-6||SGD DESC:Prephenate dehydratase, catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway Gene:PNP1(YLR209C)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Purine nucleoside phosphorylase, specifically metabolizes inosine and guanosine nucleosides; involved in the nicotinamide riboside salvage pathway Gene:RAI1(YGL246C)|FD-Score:3.29|P-value:4.98E-4||SGD DESC:Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z Gene:RRT1(YBL048W_d)|FD-Score:3.73|P-value:9.60E-5||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:RTN2(YDL204W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication Gene:STE20(YHL007C)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p Gene:SUR4(YLR372W)|FD-Score:3.88|P-value:5.19E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TPO4(YOR273C)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TRP2(YER090W)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP5(YGL026C)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TVP38(YKR088C)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:UBP11(YKR098C)|FD-Score:3.94|P-value:3.99E-5||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin from ubiquitinated proteins Gene:UBX7(YBR273C)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p; UBX7 has a paralog, UBX6, that arose from the whole genome duplication Gene:VAM3(YOR106W)|FD-Score:-3.13|P-value:8.63E-4||SGD DESC:Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region Gene:XPT1(YJR133W)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Xanthine-guanine phosphoribosyl transferase, required for xanthine utilization and for optimal utilization of guanine Gene:YDR209C(YDR209C_d)|FD-Score:3.32|P-value:4.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YDR210W. Gene:YER134C(YER134C)|FD-Score:4.59|P-value:2.18E-6||SGD DESC:Magnesium-dependent acid phosphatase, member of the haloacid dehalogenase superfamily; non-essential gene Gene:YGR121W-A(YGR121W-A_p)|FD-Score:4.77|P-value:9.24E-7||SGD DESC:Putative protein of unknown function Gene:YIL046W-A(YIL046W-A_p)|FD-Score:3.85|P-value:5.97E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YKR045C(YKR045C_p)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YKR051W(YKR051W_p)|FD-Score:7.76|P-value:4.38E-15||SGD DESC:Putative protein of unknown function Gene:YLR419W(YLR419W_p)|FD-Score:5.51|P-value:1.85E-8||SGD DESC:Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene Gene:YML037C(YML037C_p)|FD-Score:-3.32|P-value:4.57E-4||SGD DESC:Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene Gene:YMR181C(YMR181C_p)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Protein of unknown function; mRNA transcribed as part of a bicistronic transcript with a predicted transcriptional repressor RGM1/YMR182C; mRNA is destroyed by nonsense-mediated decay (NMD); not an essential gene; YMR181C has a paralog, YPL229W, that arose from the whole genome duplication Gene:YMR247W-A(YMR247W-A_p)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Putative protein of unknown function Gene:YOR225W(YOR225W_d)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR003C(YPR003C_p)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene Gene:YPT35(YHR105W)|FD-Score:3.98|P-value:3.51E-5||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport Gene:ADR1(YDR216W)|FD-Score:3.21|P-value:6.60E-4||SGD DESC:Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization Gene:BIO2(YGR286C)|FD-Score:4.18|P-value:1.43E-5||SGD DESC:Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant Gene:BRP1(YGL007W_d)|FD-Score:5.14|P-value:1.36E-7||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:BSC4(YNL269W)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p Gene:BTS1(YPL069C)|FD-Score:9.96|P-value:1.18E-23||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CWH43(YCR017C)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:FLC3(YGL139W)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Putative FAD transporter, similar to Flc1p and Flc2p; localized to the ER Gene:GCN2(YDR283C)|FD-Score:8.08|P-value:3.11E-16||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GRX6(YDL010W)|FD-Score:-3.29|P-value:4.98E-4||SGD DESC:Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication Gene:HAL1(YPR005C)|FD-Score:-4.33|P-value:7.50E-6||SGD DESC:Cytoplasmic protein involved in halotolerance; decreases intracellular Na+ (via Ena1p) and increases intracellular K+ by decreasing efflux; expression repressed by Ssn6p-Tup1p and Sko1p and induced by NaCl, KCl, and sorbitol through Gcn4p Gene:HPF1(YOL155C)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Haze-protective mannoprotein that reduces the particle size of aggregated proteins in white wines Gene:JEN1(YKL217W)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Monocarboxylate/proton symporter of the plasma membrane; transport activity is dependent on the pH gradient across the membrane; mediates high-affinity uptake of carbon sources lactate, pyuvate, and acetate, and also of the micronutrient selenite, whose structure mimics that of monocarboxylates; expression and localization are tightly regulated, with transcription repression, mRNA degradation, and protein endocytosis and degradation all occurring in the presence of glucose Gene:MNT3(YIL014W)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Alpha-1,3-mannosyltransferase, adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation Gene:MTC3(YGL226W)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1 Gene:MVP1(YMR004W)|FD-Score:-3.52|P-value:2.14E-4||SGD DESC:Protein required for sorting proteins to the vacuole; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions; protein abundance increases in response to DNA replication stress Gene:MYO4(YAL029C)|FD-Score:3.14|P-value:8.42E-4||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication Gene:PAC11(YDR488C)|FD-Score:3.73|P-value:9.57E-5||SGD DESC:Dynein intermediate chain, acts in the cytoplasmic dynein pathway, forms cortical cytoplasmic microtubule capture site with Num1p; null mutant is defective in nuclear migration, essential in the absence of CIN8 Gene:PHA2(YNL316C)|FD-Score:4.37|P-value:6.22E-6||SGD DESC:Prephenate dehydratase, catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway Gene:PNP1(YLR209C)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Purine nucleoside phosphorylase, specifically metabolizes inosine and guanosine nucleosides; involved in the nicotinamide riboside salvage pathway Gene:RAI1(YGL246C)|FD-Score:3.29|P-value:4.98E-4||SGD DESC:Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z Gene:RRT1(YBL048W_d)|FD-Score:3.73|P-value:9.60E-5||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:RTN2(YDL204W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication Gene:STE20(YHL007C)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p Gene:SUR4(YLR372W)|FD-Score:3.88|P-value:5.19E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TPO4(YOR273C)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TRP2(YER090W)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP5(YGL026C)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TVP38(YKR088C)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:UBP11(YKR098C)|FD-Score:3.94|P-value:3.99E-5||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin from ubiquitinated proteins Gene:UBX7(YBR273C)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p; UBX7 has a paralog, UBX6, that arose from the whole genome duplication Gene:VAM3(YOR106W)|FD-Score:-3.13|P-value:8.63E-4||SGD DESC:Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region Gene:XPT1(YJR133W)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Xanthine-guanine phosphoribosyl transferase, required for xanthine utilization and for optimal utilization of guanine Gene:YDR209C(YDR209C_d)|FD-Score:3.32|P-value:4.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YDR210W. Gene:YER134C(YER134C)|FD-Score:4.59|P-value:2.18E-6||SGD DESC:Magnesium-dependent acid phosphatase, member of the haloacid dehalogenase superfamily; non-essential gene Gene:YGR121W-A(YGR121W-A_p)|FD-Score:4.77|P-value:9.24E-7||SGD DESC:Putative protein of unknown function Gene:YIL046W-A(YIL046W-A_p)|FD-Score:3.85|P-value:5.97E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YKR045C(YKR045C_p)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YKR051W(YKR051W_p)|FD-Score:7.76|P-value:4.38E-15||SGD DESC:Putative protein of unknown function Gene:YLR419W(YLR419W_p)|FD-Score:5.51|P-value:1.85E-8||SGD DESC:Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene Gene:YML037C(YML037C_p)|FD-Score:-3.32|P-value:4.57E-4||SGD DESC:Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene Gene:YMR181C(YMR181C_p)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Protein of unknown function; mRNA transcribed as part of a bicistronic transcript with a predicted transcriptional repressor RGM1/YMR182C; mRNA is destroyed by nonsense-mediated decay (NMD); not an essential gene; YMR181C has a paralog, YPL229W, that arose from the whole genome duplication Gene:YMR247W-A(YMR247W-A_p)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Putative protein of unknown function Gene:YOR225W(YOR225W_d)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR003C(YPR003C_p)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene Gene:YPT35(YHR105W)|FD-Score:3.98|P-value:3.51E-5||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR367W3.797.39E-50.32KEI1Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
YNR046W3.472.59E-40.02TRM112Protein involved in methylation of tRNA, rRNA, and translation factors; subunit of tRNA methyltransferase (MTase) complexes in combination with Trm9p and Trm11p; interacts with and stabilizes 18S rRNA methyltransferase Bud23p; subunit of complex with Mtq2p that methylates Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; deletion confers resistance to zymocin; relative distribution to the nucleus increases upon DNA replication stress
YDR328C3.452.79E-40.11SKP1Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress
YFL037W3.344.15E-40.15TUB2Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria
YBL076C3.206.98E-40.07ILS1Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A
YLR440C3.128.96E-40.05SEC39Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope
YHR186C3.070.001060.04KOG1Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
YKR086W3.040.001200.10PRP16DEAH-box RNA helicase involved in second catalytic step of splicing; exhibits ATP-dependent RNA unwinding activity; mediates the release of Yju2p and Cwc25p in the second step; in the absence of ATP, stabilizes the binding of Cwc25p to the spliceosome in the first catalytic step
YOR075W2.940.001640.19UFE1t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER
YOR310C2.750.002990.02NOP58Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA
YKL013C2.730.003190.07ARC19Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YDL209C2.650.003980.00CWC2Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2
YGL128C2.650.003990.04CWC23Component of a complex containing Cef1p, putatively involved in pre-mRNA splicing; has similarity to E. coli DnaJ and other DnaJ-like proteins and to S. pombe Cwf23p
YGR277C2.610.004560.04CAB4Probable pantetheine-phosphate adenylyltransferase (PPAT), which catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; null mutant lethality is complemented by E. coli coaD (encoding PPAT); widely conserved
YHR197W2.570.005090.08RIX1Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPL069C9.961.18E-23BTS1Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
YDR283C8.083.11E-16GCN2Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control
YKR051W_p7.764.38E-15YKR051W_pPutative protein of unknown function
YLR419W_p5.511.85E-8YLR419W_pPutative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene
YGL007W_d5.141.36E-7BRP1_dDubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1
YGR121W-A_p4.779.24E-7YGR121W-A_pPutative protein of unknown function
YER134C4.592.18E-6YER134CMagnesium-dependent acid phosphatase, member of the haloacid dehalogenase superfamily; non-essential gene
YNL316C4.376.22E-6PHA2Prephenate dehydratase, catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway
YKL217W4.221.20E-5JEN1Monocarboxylate/proton symporter of the plasma membrane; transport activity is dependent on the pH gradient across the membrane; mediates high-affinity uptake of carbon sources lactate, pyuvate, and acetate, and also of the micronutrient selenite, whose structure mimics that of monocarboxylates; expression and localization are tightly regulated, with transcription repression, mRNA degradation, and protein endocytosis and degradation all occurring in the presence of glucose
YGR286C4.181.43E-5BIO2Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant
YHR105W3.983.51E-5YPT35Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport
YKR098C3.943.99E-5UBP11Ubiquitin-specific protease that cleaves ubiquitin from ubiquitinated proteins
YLR372W3.885.19E-5SUR4Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis
YIL046W-A_p3.855.97E-5YIL046W-A_pPutative protein of unknown function; identified by expression profiling and mass spectrometry
YDR488C3.739.57E-5PAC11Dynein intermediate chain, acts in the cytoplasmic dynein pathway, forms cortical cytoplasmic microtubule capture site with Num1p; null mutant is defective in nuclear migration, essential in the absence of CIN8

GO enrichment analysis for SGTC_296
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1701.76E-39SGTC_1764st044502 93.5 μMTimTec (Natural product derivative library)84070.0769231
0.1553.32E-33SGTC_6770929-0063 136.0 μMChemDiv (Drug-like library)15395830.101695
0.1512.54E-31SGTC_33089128332 17.6 μMChembridge (Drug-like library)173328050.0724638plasma membrane duress
0.1498.28E-31SGTC_24725377652 49.4 μMMiscellaneous16109030.137931TRP & mitochondrial translation
0.1426.92E-28SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.0819672
0.1381.21E-26SGTC_18815652484 16.0 μMMiscellaneous22551290.0958904TRP & mitochondrial translation
0.1351.83E-25SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.0615385
0.1301.03E-23SGTC_1524469-0003 551.2 μMChemDiv (Drug-like library)6972800.0677966
0.1248.41E-22SGTC_9591222-0044 107.0 μMChemDiv (Drug-like library)60836120.909091
0.1232.35E-21SGTC_20384100038 31.0 μMChembridge (Fragment library)35968030.0526316plasma membrane duress
0.1232.74E-21SGTC_7273032-0951 202.0 μMChemDiv (Drug-like library)36528360.0923077
0.1219.21E-21SGTC_8191013-0258 27.7 μMChemDiv (Drug-like library)57531980.0985916
0.1193.79E-20SGTC_12191469-0211 33.1 μMChemDiv (Drug-like library)68222820.166667
0.1186.78E-20SGTC_18835-benzylidenerhodanine 20.0 μMMiscellaneous12732100.0925926TRP & mitochondrial translation
0.1189.30E-20SGTC_24835729050 27.2 μMMiscellaneous28651800.15942

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_9591222-0044107 μM0.9090916083612ChemDiv (Drug-like library)232.31814.68512
SGTC_9581222-000733 μM0.8823537312461ChemDiv (Drug-like library)246.344685.14112
SGTC_790052-000794.09 μM0.52801472ChemDiv (Drug-like library)276.370664.63813
SGTC_810411-00469.97 μM0.46341512925265ChemDiv (Drug-like library)297.230645.4701
SGTC_820054-0107293.76 μM0.4565223104697ChemDiv (Drug-like library)340.412866.11514
SGTC_1121fusaric acid630.51 nM0.4523813442TimTec (Natural product library)179.215722.59213
SGTC_5331222-02226.71 μM0.4468093982874ChemDiv (Drug-like library)324.37045.58204PDR1
SGTC_3027908954149.47 μM0.4347839943819Chembridge (Drug-like library)288.427723.92802fatty acid desaturase (OLE1)
SGTC_3035909130349.47 μM0.42553225237002Chembridge (Drug-like library)275.38593.54102
SGTC_2969909140828.7 μM0.4081634286311Chembridge (Drug-like library)254.326923.31812