9082772

N-(3-methylphenyl)-2-(2-oxo-3,4-dihydro-1,4-benzoxazin-3-yl)acetamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2965
Screen concentration 38.6 μM
Source Chembridge (Drug-like library)
PubChem CID 16459634
SMILES CC1=CC(=CC=C1)NC(=O)CC2C(=O)OC3=CC=CC=C3N2
Standardized SMILES Cc1cccc(NC(=O)CC2Nc3ccccc3OC2=O)c1
Molecular weight 296.3205
ALogP 2.6
H-bond donor count 2
H-bond acceptor count 4
Response signature mitochondrial response to ROS

Pool Growth Kinetics
% growth inhibition (Het. pool) 63.72
% growth inhibition (Hom. pool) 8.67


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 16459634
Download HIP data (tab-delimited text)  (excel)
Gene:AFG2(YLR397C)|FD-Score:-4.56|P-value:2.60E-6|Clearance:0||SGD DESC:ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs Gene:ALA1(YOR335C)|FD-Score:-5.53|P-value:1.59E-8|Clearance:0||SGD DESC:Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog Gene:BET5(YML077W)|FD-Score:-6.49|P-value:4.27E-11|Clearance:0||SGD DESC:Component of the TRAPP (transport protein particle) complex, which plays an essential role in the vesicular transport from endoplasmic reticulum to Golgi Gene:CAB2(YIL083C)|FD-Score:-4.63|P-value:1.84E-6|Clearance:0||SGD DESC:Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity) Gene:CDC11(YJR076C)|FD-Score:-4.26|P-value:1.01E-5|Clearance:0||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC13(YDL220C)|FD-Score:-4.23|P-value:1.15E-5|Clearance:0||SGD DESC:Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation Gene:CDC20(YGL116W)|FD-Score:-4.84|P-value:6.44E-7|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC23(YHR166C)|FD-Score:-4.63|P-value:1.86E-6|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CDC25(YLR310C)|FD-Score:-4.47|P-value:3.87E-6|Clearance:0||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:CDC33(YOL139C)|FD-Score:-4.79|P-value:8.44E-7|Clearance:0||SGD DESC:mRNA cap binding protein and translation initiation factor eIF4E; the eIF4E-cap complex is responsible for mediating cap-dependent mRNA translation via interactions with translation initiation factor eIF4G (Tif4631p or Tif4632p); protein abundance increases in response to DNA replication stress; mutants are defective for adhesion and pseudohyphal growth Gene:CDC9(YDL164C)|FD-Score:-4.02|P-value:2.89E-5|Clearance:0||SGD DESC:DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination Gene:CNS1(YBR155W)|FD-Score:-4.69|P-value:1.34E-6|Clearance:0||SGD DESC:TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion Gene:CYR1(YJL005W)|FD-Score:-3.23|P-value:6.26E-4|Clearance:0||SGD DESC:Adenylate cyclase, required for cAMP production and cAMP-dependent protein kinase signaling; the cAMP pathway controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation Gene:DCP1(YOL149W)|FD-Score:-3.1|P-value:9.56E-4|Clearance:0||SGD DESC:Subunit of the Dcp1p-Dcp2p decapping enzyme complex; decapping complex removes the 5' cap structure from mRNAs prior to their degradation; enhances the activity of catalytic subunit Dcp2p; regulated by DEAD box protein Dhh1p; forms cytoplasmic foci upon DNA replication stress Gene:DED81(YHR019C)|FD-Score:-3.55|P-value:1.89E-4|Clearance:0||SGD DESC:Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA Gene:ERG20(YJL167W)|FD-Score:-6.54|P-value:2.98E-11|Clearance:0||SGD DESC:Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis Gene:FAL1(YDR021W)|FD-Score:-5.45|P-value:2.52E-8|Clearance:0||SGD DESC:Nucleolar protein required for maturation of 18S rRNA, member of the eIF4A subfamily of DEAD-box ATP-dependent RNA helicases Gene:FAS2(YPL231W)|FD-Score:-3.97|P-value:3.57E-5|Clearance:0||SGD DESC:Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities Gene:GLC7(YER133W)|FD-Score:-8.68|P-value:1.93E-18|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GRS1(YBR121C)|FD-Score:-3.25|P-value:5.80E-4|Clearance:0||SGD DESC:Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication Gene:HCA4(YJL033W)|FD-Score:-3.81|P-value:6.86E-5|Clearance:0||SGD DESC:DEAD box RNA helicase; high-copy number suppression of a U14 snoRNA processing mutant suggests an involvement in 18S rRNA synthesis Gene:HEM12(YDR047W)|FD-Score:3.83|P-value:6.35E-5|Clearance:0.68||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HRR25(YPL204W)|FD-Score:3.97|P-value:3.59E-5|Clearance:0.68||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:HSF1(YGL073W)|FD-Score:-3.5|P-value:2.37E-4|Clearance:0||SGD DESC:Trimeric heat shock transcription factor, activates multiple genes in response to stresses that include hyperthermia; recognizes variable heat shock elements (HSEs) consisting of inverted NGAAN repeats; posttranslationally regulated Gene:HSH49(YOR319W)|FD-Score:-3.7|P-value:1.06E-4|Clearance:0||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:IFH1(YLR223C)|FD-Score:-5.36|P-value:4.16E-8|Clearance:0||SGD DESC:Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing Gene:IQG1(YPL242C)|FD-Score:-7.97|P-value:8.23E-16|Clearance:0||SGD DESC:Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:JAC1(YGL018C)|FD-Score:-4.03|P-value:2.80E-5|Clearance:0||SGD DESC:Specialized J-protein that functions with Hsp70 in Fe-S cluster biogenesis in mitochondria, involved in iron metabolism; contains a J domain typical to J-type chaperones; localizes to the mitochondrial matrix Gene:LCD1(YDR499W)|FD-Score:-5.3|P-value:5.83E-8|Clearance:0||SGD DESC:Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP; forms nuclear foci upon DNA replication stress Gene:LSM2(YBL026W)|FD-Score:-3.24|P-value:6.05E-4|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:LSM3(YLR438C-A)|FD-Score:-12|P-value:1.54E-33|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:LSM4(YER112W)|FD-Score:-5.59|P-value:1.12E-8|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:MCD1(YDL003W)|FD-Score:-6.31|P-value:1.38E-10|Clearance:0||SGD DESC:Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase Gene:MCM1(YMR043W)|FD-Score:-4.07|P-value:2.33E-5|Clearance:0||SGD DESC:Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes Gene:MCM10(YIL150C)|FD-Score:-8.16|P-value:1.67E-16|Clearance:0||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:NCB2(YDR397C)|FD-Score:-5.42|P-value:3.03E-8|Clearance:0||SGD DESC:Subunit of a heterodimeric NC2 transcription regulator complex with Bur6p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2beta Gene:NFS1(YCL017C)|FD-Score:-7.4|P-value:6.76E-14|Clearance:0||SGD DESC:Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria Gene:NOB1(YOR056C)|FD-Score:-3.49|P-value:2.43E-4|Clearance:0||SGD DESC:Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:NOP58(YOR310C)|FD-Score:-3.48|P-value:2.52E-4|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:NOP8(YOL144W)|FD-Score:-3.32|P-value:4.57E-4|Clearance:0||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:NUF2(YOL069W)|FD-Score:-4.52|P-value:3.09E-6|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:NUG1(YER006W)|FD-Score:3.15|P-value:8.20E-4|Clearance:0.11||SGD DESC:GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus Gene:NUT2(YPR168W)|FD-Score:-4.78|P-value:8.96E-7|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress Gene:ORC2(YBR060C)|FD-Score:-3.22|P-value:6.33E-4|Clearance:0||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p Gene:PGA2(YNL149C)|FD-Score:-3.37|P-value:3.79E-4|Clearance:0||SGD DESC:Essential protein required for maturation of Gas1p and Pho8p; involved in protein trafficking; GFP-fusion protein localizes to the ER and YFP-fusion protein to the nuclear envelope-ER network; null mutants have a cell separation defect Gene:POB3(YML069W)|FD-Score:-4.15|P-value:1.68E-5|Clearance:0||SGD DESC:Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; protein abundance increases in response to DNA replication stress Gene:POL12(YBL035C)|FD-Score:-4.07|P-value:2.35E-5|Clearance:0||SGD DESC:B subunit of DNA polymerase alpha-primase complex, required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation Gene:POL3(YDL102W)|FD-Score:-8.65|P-value:2.57E-18|Clearance:0||SGD DESC:Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER) Gene:PRE1(YER012W)|FD-Score:-4.53|P-value:2.90E-6|Clearance:0||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:PRE10(YOR362C)|FD-Score:-3.42|P-value:3.12E-4|Clearance:0||SGD DESC:Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRE2(YPR103W)|FD-Score:-4.12|P-value:1.93E-5|Clearance:0||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:PRE4(YFR050C)|FD-Score:-7.56|P-value:2.00E-14|Clearance:0||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:PRE5(YMR314W)|FD-Score:-4.02|P-value:2.90E-5|Clearance:0||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRE7(YBL041W)|FD-Score:-10.4|P-value:1.39E-25|Clearance:0||SGD DESC:Beta 6 subunit of the 20S proteasome Gene:PRE8(YML092C)|FD-Score:-3.28|P-value:5.17E-4|Clearance:0||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:PRP40(YKL012W)|FD-Score:-5.1|P-value:1.67E-7|Clearance:0||SGD DESC:U1 snRNP protein involved in splicing, interacts with the branchpoint-binding protein during the formation of the second commitment complex Gene:PSE1(YMR308C)|FD-Score:-9.74|P-value:1.01E-22|Clearance:0||SGD DESC:Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p Gene:PUP2(YGR253C)|FD-Score:-4.31|P-value:8.10E-6|Clearance:0||SGD DESC:Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta Gene:PUP3(YER094C)|FD-Score:-3.76|P-value:8.62E-5|Clearance:0||SGD DESC:Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10 Gene:RAM2(YKL019W)|FD-Score:-4.99|P-value:3.02E-7|Clearance:0||SGD DESC:Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor Gene:RDS3(YPR094W)|FD-Score:-5.12|P-value:1.51E-7|Clearance:0||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP, zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance Gene:RFA3(YJL173C)|FD-Score:-4.62|P-value:1.92E-6|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress Gene:RFT1(YBL020W)|FD-Score:-5.15|P-value:1.28E-7|Clearance:0||SGD DESC:Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein Gene:RGR1(YLR071C)|FD-Score:-4.77|P-value:9.11E-7|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for glucose repression, HO repression, RME1 repression and sporulation Gene:RIX1(YHR197W)|FD-Score:-3.27|P-value:5.41E-4|Clearance:0||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene Gene:RPL25(YOL127W)|FD-Score:-3.44|P-value:2.94E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPL32(YBL092W)|FD-Score:-3.2|P-value:6.87E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPN11(YFR004W)|FD-Score:-4.31|P-value:8.28E-6|Clearance:0||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:RPN6(YDL097C)|FD-Score:-5.12|P-value:1.56E-7|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:RPN9(YDR427W)|FD-Score:-3.53|P-value:2.11E-4|Clearance:0||SGD DESC:Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress Gene:RPS13(YDR064W)|FD-Score:-5.81|P-value:3.14E-9|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:RPS20(YHL015W)|FD-Score:-4.02|P-value:2.93E-5|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RPS3(YNL178W)|FD-Score:-3.17|P-value:7.72E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RPT2(YDL007W)|FD-Score:-6.03|P-value:8.08E-10|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle Gene:RPT5(YOR117W)|FD-Score:-3.78|P-value:7.73E-5|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:RPT6(YGL048C)|FD-Score:-4.08|P-value:2.29E-5|Clearance:0||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:RRB1(YMR131C)|FD-Score:-5.97|P-value:1.18E-9|Clearance:0||SGD DESC:Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p Gene:RRN9(YMR270C)|FD-Score:-4.55|P-value:2.66E-6|Clearance:0||SGD DESC:Protein involved in promoting high level transcription of rDNA, subunit of UAF (upstream activation factor) for RNA polymerase I Gene:RRP9(YPR137W)|FD-Score:-4.74|P-value:1.07E-6|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:SCL1(YGL011C)|FD-Score:-4.77|P-value:9.22E-7|Clearance:0||SGD DESC:Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria Gene:SCM3(YDL139C)|FD-Score:-3.1|P-value:9.65E-4|Clearance:0||SGD DESC:Nonhistone component of centromeric chromatin; binds stoichiometrically to CenH3-H4 histones, required for kinetochore assembly; required for G2/M progression and localization of Cse4p; may protect Cse4p from ubiquitylation; homolog of mammalian HJURP Gene:SEC27(YGL137W)|FD-Score:-10.6|P-value:1.23E-26|Clearance:0||SGD DESC:Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP Gene:SEC31(YDL195W)|FD-Score:-3.9|P-value:4.72E-5|Clearance:0||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SEC53(YFL045C)|FD-Score:-4.14|P-value:1.75E-5|Clearance:0||SGD DESC:Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen Gene:SEC7(YDR170C)|FD-Score:-5|P-value:2.90E-7|Clearance:0||SGD DESC:Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles Gene:SEN34(YAR008W)|FD-Score:-5.7|P-value:5.83E-9|Clearance:0||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease Gene:SES1(YDR023W)|FD-Score:-4.22|P-value:1.21E-5|Clearance:0||SGD DESC:Cytosolic seryl-tRNA synthetase, class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), displays tRNA-dependent amino acid recognition which enhances discrimination of the serine substrate, interacts with peroxin Pex21p Gene:SLN1(YIL147C)|FD-Score:-4.45|P-value:4.37E-6|Clearance:0||SGD DESC:Histidine kinase osmosensor that regulates a MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators Gene:SMD2(YLR275W)|FD-Score:-3.68|P-value:1.16E-4|Clearance:0||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:SNM1(YDR478W)|FD-Score:-11.8|P-value:1.68E-32|Clearance:0||SGD DESC:Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP Gene:SSL2(YIL143C)|FD-Score:-6.27|P-value:1.86E-10|Clearance:0||SGD DESC:Component of RNA polymerase transcription factor TFIIH holoenzyme; has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; interacts functionally with TFIIB and has roles in transcription start site selection and in gene looping to juxtapose initiation and termination regions; involved in DNA repair; homolog of human ERCC3 Gene:SSY5(YJL156C)|FD-Score:-5.71|P-value:5.51E-9|Clearance:0||SGD DESC:Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p Gene:STU1(YBL034C)|FD-Score:-4.03|P-value:2.75E-5|Clearance:0||SGD DESC:Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles Gene:SYF1(YDR416W)|FD-Score:-5.77|P-value:3.95E-9|Clearance:0||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; homologs in human and C. elegans Gene:TAF6(YGL112C)|FD-Score:-5.66|P-value:7.62E-9|Clearance:0||SGD DESC:Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4 Gene:TAM41(YGR046W)|FD-Score:-5.24|P-value:7.89E-8|Clearance:0||SGD DESC:Mitochondrial protein required for cardiolipin biosynthesis; viability of null mutant is strain-dependent; mRNA is targeted to the bud; mutant displays defect in mitochondrial protein import, likely due to altered membrane lipid composition Gene:TFB1(YDR311W)|FD-Score:-3.38|P-value:3.57E-4|Clearance:0||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators Gene:THS1(YIL078W)|FD-Score:-4.68|P-value:1.44E-6|Clearance:0||SGD DESC:Threonyl-tRNA synthetase, essential cytoplasmic protein Gene:TIM17(YJL143W)|FD-Score:-5.19|P-value:1.04E-7|Clearance:0||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:TIM23(YNR017W)|FD-Score:-5.23|P-value:8.58E-8|Clearance:0||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel Gene:TSR1(YDL060W)|FD-Score:-8.21|P-value:1.09E-16|Clearance:0||SGD DESC:Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:TUB1(YML085C)|FD-Score:-4.43|P-value:4.64E-6|Clearance:0||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:UTP13(YLR222C)|FD-Score:-3.6|P-value:1.58E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:UTP4(YDR324C)|FD-Score:-3.15|P-value:8.18E-4|Clearance:0||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:YBR089W(YBR089W_d)|FD-Score:-4.42|P-value:4.95E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene POL30 Gene:YCR013C(YCR013C_d)|FD-Score:-4.5|P-value:3.34E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YCR013C and the overlapping essential gene PGK1 is reduced in a gcr1 null mutant Gene:YGL074C(YGL074C_d)|FD-Score:3.93|P-value:4.29E-5|Clearance:0.68||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor Gene:YKL036C(YKL036C_d)|FD-Score:-4.4|P-value:5.51E-6|Clearance:0||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W Gene:YKT6(YKL196C)|FD-Score:-8.44|P-value:1.58E-17|Clearance:0||SGD DESC:Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus Gene:YOR218C(YOR218C_d)|FD-Score:-7.36|P-value:9.06E-14|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene RFC1/YOR217W Gene:YPD1(YDL235C)|FD-Score:-3.28|P-value:5.28E-4|Clearance:0||SGD DESC:Phosphorelay intermediate protein, phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus Gene:YRA1(YDR381W)|FD-Score:-5.06|P-value:2.13E-7|Clearance:0||SGD DESC:RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member Gene:AFG2(YLR397C)|FD-Score:-4.56|P-value:2.60E-6|Clearance:0||SGD DESC:ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs Gene:ALA1(YOR335C)|FD-Score:-5.53|P-value:1.59E-8|Clearance:0||SGD DESC:Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog Gene:BET5(YML077W)|FD-Score:-6.49|P-value:4.27E-11|Clearance:0||SGD DESC:Component of the TRAPP (transport protein particle) complex, which plays an essential role in the vesicular transport from endoplasmic reticulum to Golgi Gene:CAB2(YIL083C)|FD-Score:-4.63|P-value:1.84E-6|Clearance:0||SGD DESC:Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity) Gene:CDC11(YJR076C)|FD-Score:-4.26|P-value:1.01E-5|Clearance:0||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC13(YDL220C)|FD-Score:-4.23|P-value:1.15E-5|Clearance:0||SGD DESC:Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation Gene:CDC20(YGL116W)|FD-Score:-4.84|P-value:6.44E-7|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC23(YHR166C)|FD-Score:-4.63|P-value:1.86E-6|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CDC25(YLR310C)|FD-Score:-4.47|P-value:3.87E-6|Clearance:0||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:CDC33(YOL139C)|FD-Score:-4.79|P-value:8.44E-7|Clearance:0||SGD DESC:mRNA cap binding protein and translation initiation factor eIF4E; the eIF4E-cap complex is responsible for mediating cap-dependent mRNA translation via interactions with translation initiation factor eIF4G (Tif4631p or Tif4632p); protein abundance increases in response to DNA replication stress; mutants are defective for adhesion and pseudohyphal growth Gene:CDC9(YDL164C)|FD-Score:-4.02|P-value:2.89E-5|Clearance:0||SGD DESC:DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination Gene:CNS1(YBR155W)|FD-Score:-4.69|P-value:1.34E-6|Clearance:0||SGD DESC:TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion Gene:CYR1(YJL005W)|FD-Score:-3.23|P-value:6.26E-4|Clearance:0||SGD DESC:Adenylate cyclase, required for cAMP production and cAMP-dependent protein kinase signaling; the cAMP pathway controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation Gene:DCP1(YOL149W)|FD-Score:-3.1|P-value:9.56E-4|Clearance:0||SGD DESC:Subunit of the Dcp1p-Dcp2p decapping enzyme complex; decapping complex removes the 5' cap structure from mRNAs prior to their degradation; enhances the activity of catalytic subunit Dcp2p; regulated by DEAD box protein Dhh1p; forms cytoplasmic foci upon DNA replication stress Gene:DED81(YHR019C)|FD-Score:-3.55|P-value:1.89E-4|Clearance:0||SGD DESC:Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA Gene:ERG20(YJL167W)|FD-Score:-6.54|P-value:2.98E-11|Clearance:0||SGD DESC:Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis Gene:FAL1(YDR021W)|FD-Score:-5.45|P-value:2.52E-8|Clearance:0||SGD DESC:Nucleolar protein required for maturation of 18S rRNA, member of the eIF4A subfamily of DEAD-box ATP-dependent RNA helicases Gene:FAS2(YPL231W)|FD-Score:-3.97|P-value:3.57E-5|Clearance:0||SGD DESC:Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities Gene:GLC7(YER133W)|FD-Score:-8.68|P-value:1.93E-18|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GRS1(YBR121C)|FD-Score:-3.25|P-value:5.80E-4|Clearance:0||SGD DESC:Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication Gene:HCA4(YJL033W)|FD-Score:-3.81|P-value:6.86E-5|Clearance:0||SGD DESC:DEAD box RNA helicase; high-copy number suppression of a U14 snoRNA processing mutant suggests an involvement in 18S rRNA synthesis Gene:HEM12(YDR047W)|FD-Score:3.83|P-value:6.35E-5|Clearance:0.68||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HRR25(YPL204W)|FD-Score:3.97|P-value:3.59E-5|Clearance:0.68||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:HSF1(YGL073W)|FD-Score:-3.5|P-value:2.37E-4|Clearance:0||SGD DESC:Trimeric heat shock transcription factor, activates multiple genes in response to stresses that include hyperthermia; recognizes variable heat shock elements (HSEs) consisting of inverted NGAAN repeats; posttranslationally regulated Gene:HSH49(YOR319W)|FD-Score:-3.7|P-value:1.06E-4|Clearance:0||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:IFH1(YLR223C)|FD-Score:-5.36|P-value:4.16E-8|Clearance:0||SGD DESC:Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing Gene:IQG1(YPL242C)|FD-Score:-7.97|P-value:8.23E-16|Clearance:0||SGD DESC:Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:JAC1(YGL018C)|FD-Score:-4.03|P-value:2.80E-5|Clearance:0||SGD DESC:Specialized J-protein that functions with Hsp70 in Fe-S cluster biogenesis in mitochondria, involved in iron metabolism; contains a J domain typical to J-type chaperones; localizes to the mitochondrial matrix Gene:LCD1(YDR499W)|FD-Score:-5.3|P-value:5.83E-8|Clearance:0||SGD DESC:Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP; forms nuclear foci upon DNA replication stress Gene:LSM2(YBL026W)|FD-Score:-3.24|P-value:6.05E-4|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:LSM3(YLR438C-A)|FD-Score:-12|P-value:1.54E-33|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:LSM4(YER112W)|FD-Score:-5.59|P-value:1.12E-8|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:MCD1(YDL003W)|FD-Score:-6.31|P-value:1.38E-10|Clearance:0||SGD DESC:Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase Gene:MCM1(YMR043W)|FD-Score:-4.07|P-value:2.33E-5|Clearance:0||SGD DESC:Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes Gene:MCM10(YIL150C)|FD-Score:-8.16|P-value:1.67E-16|Clearance:0||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:NCB2(YDR397C)|FD-Score:-5.42|P-value:3.03E-8|Clearance:0||SGD DESC:Subunit of a heterodimeric NC2 transcription regulator complex with Bur6p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2beta Gene:NFS1(YCL017C)|FD-Score:-7.4|P-value:6.76E-14|Clearance:0||SGD DESC:Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria Gene:NOB1(YOR056C)|FD-Score:-3.49|P-value:2.43E-4|Clearance:0||SGD DESC:Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:NOP58(YOR310C)|FD-Score:-3.48|P-value:2.52E-4|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:NOP8(YOL144W)|FD-Score:-3.32|P-value:4.57E-4|Clearance:0||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:NUF2(YOL069W)|FD-Score:-4.52|P-value:3.09E-6|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:NUG1(YER006W)|FD-Score:3.15|P-value:8.20E-4|Clearance:0.11||SGD DESC:GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus Gene:NUT2(YPR168W)|FD-Score:-4.78|P-value:8.96E-7|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress Gene:ORC2(YBR060C)|FD-Score:-3.22|P-value:6.33E-4|Clearance:0||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p Gene:PGA2(YNL149C)|FD-Score:-3.37|P-value:3.79E-4|Clearance:0||SGD DESC:Essential protein required for maturation of Gas1p and Pho8p; involved in protein trafficking; GFP-fusion protein localizes to the ER and YFP-fusion protein to the nuclear envelope-ER network; null mutants have a cell separation defect Gene:POB3(YML069W)|FD-Score:-4.15|P-value:1.68E-5|Clearance:0||SGD DESC:Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; protein abundance increases in response to DNA replication stress Gene:POL12(YBL035C)|FD-Score:-4.07|P-value:2.35E-5|Clearance:0||SGD DESC:B subunit of DNA polymerase alpha-primase complex, required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation Gene:POL3(YDL102W)|FD-Score:-8.65|P-value:2.57E-18|Clearance:0||SGD DESC:Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER) Gene:PRE1(YER012W)|FD-Score:-4.53|P-value:2.90E-6|Clearance:0||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:PRE10(YOR362C)|FD-Score:-3.42|P-value:3.12E-4|Clearance:0||SGD DESC:Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRE2(YPR103W)|FD-Score:-4.12|P-value:1.93E-5|Clearance:0||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:PRE4(YFR050C)|FD-Score:-7.56|P-value:2.00E-14|Clearance:0||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:PRE5(YMR314W)|FD-Score:-4.02|P-value:2.90E-5|Clearance:0||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRE7(YBL041W)|FD-Score:-10.4|P-value:1.39E-25|Clearance:0||SGD DESC:Beta 6 subunit of the 20S proteasome Gene:PRE8(YML092C)|FD-Score:-3.28|P-value:5.17E-4|Clearance:0||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:PRP40(YKL012W)|FD-Score:-5.1|P-value:1.67E-7|Clearance:0||SGD DESC:U1 snRNP protein involved in splicing, interacts with the branchpoint-binding protein during the formation of the second commitment complex Gene:PSE1(YMR308C)|FD-Score:-9.74|P-value:1.01E-22|Clearance:0||SGD DESC:Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p Gene:PUP2(YGR253C)|FD-Score:-4.31|P-value:8.10E-6|Clearance:0||SGD DESC:Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta Gene:PUP3(YER094C)|FD-Score:-3.76|P-value:8.62E-5|Clearance:0||SGD DESC:Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10 Gene:RAM2(YKL019W)|FD-Score:-4.99|P-value:3.02E-7|Clearance:0||SGD DESC:Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor Gene:RDS3(YPR094W)|FD-Score:-5.12|P-value:1.51E-7|Clearance:0||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP, zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance Gene:RFA3(YJL173C)|FD-Score:-4.62|P-value:1.92E-6|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress Gene:RFT1(YBL020W)|FD-Score:-5.15|P-value:1.28E-7|Clearance:0||SGD DESC:Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein Gene:RGR1(YLR071C)|FD-Score:-4.77|P-value:9.11E-7|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for glucose repression, HO repression, RME1 repression and sporulation Gene:RIX1(YHR197W)|FD-Score:-3.27|P-value:5.41E-4|Clearance:0||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene Gene:RPL25(YOL127W)|FD-Score:-3.44|P-value:2.94E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPL32(YBL092W)|FD-Score:-3.2|P-value:6.87E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPN11(YFR004W)|FD-Score:-4.31|P-value:8.28E-6|Clearance:0||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:RPN6(YDL097C)|FD-Score:-5.12|P-value:1.56E-7|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:RPN9(YDR427W)|FD-Score:-3.53|P-value:2.11E-4|Clearance:0||SGD DESC:Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress Gene:RPS13(YDR064W)|FD-Score:-5.81|P-value:3.14E-9|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:RPS20(YHL015W)|FD-Score:-4.02|P-value:2.93E-5|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RPS3(YNL178W)|FD-Score:-3.17|P-value:7.72E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RPT2(YDL007W)|FD-Score:-6.03|P-value:8.08E-10|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle Gene:RPT5(YOR117W)|FD-Score:-3.78|P-value:7.73E-5|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:RPT6(YGL048C)|FD-Score:-4.08|P-value:2.29E-5|Clearance:0||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:RRB1(YMR131C)|FD-Score:-5.97|P-value:1.18E-9|Clearance:0||SGD DESC:Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p Gene:RRN9(YMR270C)|FD-Score:-4.55|P-value:2.66E-6|Clearance:0||SGD DESC:Protein involved in promoting high level transcription of rDNA, subunit of UAF (upstream activation factor) for RNA polymerase I Gene:RRP9(YPR137W)|FD-Score:-4.74|P-value:1.07E-6|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:SCL1(YGL011C)|FD-Score:-4.77|P-value:9.22E-7|Clearance:0||SGD DESC:Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria Gene:SCM3(YDL139C)|FD-Score:-3.1|P-value:9.65E-4|Clearance:0||SGD DESC:Nonhistone component of centromeric chromatin; binds stoichiometrically to CenH3-H4 histones, required for kinetochore assembly; required for G2/M progression and localization of Cse4p; may protect Cse4p from ubiquitylation; homolog of mammalian HJURP Gene:SEC27(YGL137W)|FD-Score:-10.6|P-value:1.23E-26|Clearance:0||SGD DESC:Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP Gene:SEC31(YDL195W)|FD-Score:-3.9|P-value:4.72E-5|Clearance:0||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SEC53(YFL045C)|FD-Score:-4.14|P-value:1.75E-5|Clearance:0||SGD DESC:Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen Gene:SEC7(YDR170C)|FD-Score:-5|P-value:2.90E-7|Clearance:0||SGD DESC:Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles Gene:SEN34(YAR008W)|FD-Score:-5.7|P-value:5.83E-9|Clearance:0||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease Gene:SES1(YDR023W)|FD-Score:-4.22|P-value:1.21E-5|Clearance:0||SGD DESC:Cytosolic seryl-tRNA synthetase, class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), displays tRNA-dependent amino acid recognition which enhances discrimination of the serine substrate, interacts with peroxin Pex21p Gene:SLN1(YIL147C)|FD-Score:-4.45|P-value:4.37E-6|Clearance:0||SGD DESC:Histidine kinase osmosensor that regulates a MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators Gene:SMD2(YLR275W)|FD-Score:-3.68|P-value:1.16E-4|Clearance:0||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:SNM1(YDR478W)|FD-Score:-11.8|P-value:1.68E-32|Clearance:0||SGD DESC:Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP Gene:SSL2(YIL143C)|FD-Score:-6.27|P-value:1.86E-10|Clearance:0||SGD DESC:Component of RNA polymerase transcription factor TFIIH holoenzyme; has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; interacts functionally with TFIIB and has roles in transcription start site selection and in gene looping to juxtapose initiation and termination regions; involved in DNA repair; homolog of human ERCC3 Gene:SSY5(YJL156C)|FD-Score:-5.71|P-value:5.51E-9|Clearance:0||SGD DESC:Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p Gene:STU1(YBL034C)|FD-Score:-4.03|P-value:2.75E-5|Clearance:0||SGD DESC:Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles Gene:SYF1(YDR416W)|FD-Score:-5.77|P-value:3.95E-9|Clearance:0||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; homologs in human and C. elegans Gene:TAF6(YGL112C)|FD-Score:-5.66|P-value:7.62E-9|Clearance:0||SGD DESC:Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4 Gene:TAM41(YGR046W)|FD-Score:-5.24|P-value:7.89E-8|Clearance:0||SGD DESC:Mitochondrial protein required for cardiolipin biosynthesis; viability of null mutant is strain-dependent; mRNA is targeted to the bud; mutant displays defect in mitochondrial protein import, likely due to altered membrane lipid composition Gene:TFB1(YDR311W)|FD-Score:-3.38|P-value:3.57E-4|Clearance:0||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators Gene:THS1(YIL078W)|FD-Score:-4.68|P-value:1.44E-6|Clearance:0||SGD DESC:Threonyl-tRNA synthetase, essential cytoplasmic protein Gene:TIM17(YJL143W)|FD-Score:-5.19|P-value:1.04E-7|Clearance:0||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:TIM23(YNR017W)|FD-Score:-5.23|P-value:8.58E-8|Clearance:0||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel Gene:TSR1(YDL060W)|FD-Score:-8.21|P-value:1.09E-16|Clearance:0||SGD DESC:Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:TUB1(YML085C)|FD-Score:-4.43|P-value:4.64E-6|Clearance:0||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:UTP13(YLR222C)|FD-Score:-3.6|P-value:1.58E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:UTP4(YDR324C)|FD-Score:-3.15|P-value:8.18E-4|Clearance:0||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:YBR089W(YBR089W_d)|FD-Score:-4.42|P-value:4.95E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene POL30 Gene:YCR013C(YCR013C_d)|FD-Score:-4.5|P-value:3.34E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YCR013C and the overlapping essential gene PGK1 is reduced in a gcr1 null mutant Gene:YGL074C(YGL074C_d)|FD-Score:3.93|P-value:4.29E-5|Clearance:0.68||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor Gene:YKL036C(YKL036C_d)|FD-Score:-4.4|P-value:5.51E-6|Clearance:0||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W Gene:YKT6(YKL196C)|FD-Score:-8.44|P-value:1.58E-17|Clearance:0||SGD DESC:Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus Gene:YOR218C(YOR218C_d)|FD-Score:-7.36|P-value:9.06E-14|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene RFC1/YOR217W Gene:YPD1(YDL235C)|FD-Score:-3.28|P-value:5.28E-4|Clearance:0||SGD DESC:Phosphorelay intermediate protein, phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus Gene:YRA1(YDR381W)|FD-Score:-5.06|P-value:2.13E-7|Clearance:0||SGD DESC:RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 16459634
Download HOP data (tab-delimited text)  (excel)
Gene:ADH5(YBR145W)|FD-Score:3.85|P-value:5.84E-5||SGD DESC:Alcohol dehydrogenase isoenzyme V; involved in ethanol production; ADH5 has a paralog, ADH1, that arose from the whole genome duplication Gene:AFG1(YEL052W)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Conserved protein that may act as a chaperone in the degradation of misfolded or unassembled cytochrome c oxidase subunits; localized to matrix face of the mitochondrial inner membrane; member of the AAA family but lacks a protease domain Gene:AFT1(YGL071W)|FD-Score:-5.76|P-value:4.15E-9||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARF1(YDL192W)|FD-Score:3.2|P-value:6.86E-4||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication Gene:ARO1(YDR127W)|FD-Score:-3.23|P-value:6.15E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:-3.65|P-value:1.33E-4||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ASM4(YDL088C)|FD-Score:-6.8|P-value:5.33E-12||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication Gene:BEM2(YER155C)|FD-Score:-3.26|P-value:5.58E-4||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence Gene:BRE5(YNR051C)|FD-Score:-3.26|P-value:5.58E-4||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:CAN1(YEL063C)|FD-Score:3.19|P-value:7.01E-4||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:CAX4(YGR036C)|FD-Score:-5.59|P-value:1.14E-8||SGD DESC:Dolichyl pyrophosphate (Dol-P-P) phosphatase with a luminally oriented active site in the ER, cleaves the anhydride linkage in Dol-P-P, required for Dol-P-P-linked oligosaccharide intermediate synthesis and protein N-glycosylation Gene:CDC50(YCR094W)|FD-Score:12.5|P-value:5.77E-36||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CEM1(YER061C)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:Mitochondrial beta-keto-acyl synthase with possible role in fatty acid synthesis; required for mitochondrial respiration Gene:CTK1(YKL139W)|FD-Score:7.7|P-value:6.83E-15||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:DAP1(YPL170W)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:DRS2(YAL026C)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:ECM33(YBR078W)|FD-Score:8.36|P-value:3.21E-17||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:ERP1(YAR002C-A)|FD-Score:4.74|P-value:1.04E-6||SGD DESC:Member of the p24 family involved in ER to Golgi transport; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication Gene:FCY21(YER060W)|FD-Score:5.08|P-value:1.88E-7||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:FRE5(YOR384W)|FD-Score:3.72|P-value:9.98E-5||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRT2(YAL028W)|FD-Score:4.31|P-value:8.20E-6||SGD DESC:Tail-anchored ER membrane protein of unknown function; interacts with homolog Frt1p; promotes growth in conditions of high Na+, alkaline pH, or cell wall stress, possibly via a role in posttranslational translocation; potential Cdc28p substrate; FRT2 has a paralog, FRT1, that arose from the whole genome duplication Gene:FZO1(YBR179C)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system Gene:GCV3(YAL044C)|FD-Score:4.64|P-value:1.77E-6||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:GIT1(YCR098C)|FD-Score:3.12|P-value:8.91E-4||SGD DESC:Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability Gene:HMF1(YER057C)|FD-Score:5.05|P-value:2.18E-7||SGD DESC:Member of the p14.5 protein family with similarity to Mmf1p, functionally complements Mmf1p function when targeted to mitochondria; heat shock inducible; high-dosage growth inhibitor; forms a homotrimer in vitro Gene:HTD2(YHR067W)|FD-Score:4.89|P-value:5.03E-7||SGD DESC:Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology Gene:HUR1(YGL168W)|FD-Score:-3.21|P-value:6.73E-4||SGD DESC:Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene Gene:HVG1(YER039C)|FD-Score:4.63|P-value:1.81E-6||SGD DESC:Protein of unknown function, has homology to Vrg4p Gene:ICT1(YLR099C)|FD-Score:4.45|P-value:4.26E-6||SGD DESC:Lysophosphatidic acid acyltransferase; responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress; ICT1 has a paralog, ECM18, that arose from the whole genome duplication Gene:IMG1(YCR046C)|FD-Score:6.89|P-value:2.80E-12||SGD DESC:Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome Gene:IOC4(YMR044W)|FD-Score:4.11|P-value:2.00E-5||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing Gene:IPT1(YDR072C)|FD-Score:4.39|P-value:5.62E-6||SGD DESC:Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin Gene:JEM1(YJL073W)|FD-Score:4.82|P-value:7.25E-7||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:KCH1(YJR054W_p)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate Gene:MAC1(YMR021C)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MAL33(YBR297W)|FD-Score:3.18|P-value:7.32E-4||SGD DESC:MAL-activator protein, part of complex locus MAL3; nonfunctional in genomic reference strain S288C Gene:MCX1(YBR227C)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Mitochondrial matrix protein; putative ATP-binding chaperone with non-proteolytic function; similar to bacterial ClpX proteins Gene:MDJ1(YFL016C)|FD-Score:5.09|P-value:1.76E-7||SGD DESC:Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones Gene:MDL1(YLR188W)|FD-Score:4.89|P-value:4.94E-7||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, mediates export of peptides generated upon proteolysis of mitochondrial proteins, plays a role in the regulation of cellular resistance to oxidative stress Gene:MDY2(YOL111C)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MEF2(YJL102W)|FD-Score:3.82|P-value:6.56E-5||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MGM1(YOR211C)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Mitochondrial GTPase, present in complex with Ugo1p and Fzo1p; required for mt morphology and genome maintenance; exists as long and short form with different distributions; homolog of human OPA1 involved in autosomal dominant optic atrophy Gene:MRPL37(YBR268W)|FD-Score:-3.72|P-value:9.85E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL39(YML009C)|FD-Score:5.04|P-value:2.30E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MTM1(YGR257C)|FD-Score:5.11|P-value:1.64E-7||SGD DESC:Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor Gene:NDT80(YHR124W)|FD-Score:4.55|P-value:2.72E-6||SGD DESC:Meiosis-specific transcription factor required for exit from pachytene and for full meiotic recombination; activates middle sporulation genes; competes with Sum1p for binding to promoters containing middle sporulation elements (MSE) Gene:NGG1(YDR176W)|FD-Score:-3.47|P-value:2.65E-4||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:NKP2(YLR315W)|FD-Score:-4.92|P-value:4.34E-7||SGD DESC:Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2 Gene:NPL4(YBR170C)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; assists Cdc48p in the dislocation of misfolded, polyubiquitinated ERAD substrates that are subsequently delivered to the proteasome for degradation; also involved in the regulated destruction of resident ER membrane proteins, such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing (RUP) Gene:NPT1(YOR209C)|FD-Score:6.87|P-value:3.12E-12||SGD DESC:Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus Gene:NUP60(YAR002W)|FD-Score:-3.22|P-value:6.35E-4||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; both NUP1 and NUP60 are homologous to human NUP153 Gene:OXA1(YER154W)|FD-Score:3.95|P-value:3.85E-5||SGD DESC:Mitochondrial inner membrane insertase, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; conserved from bacteria to animals Gene:PEX2(YJL210W)|FD-Score:4.12|P-value:1.88E-5||SGD DESC:RING-finger peroxin and E3 ubiquitin ligase, peroxisomal membrane protein with a C-terminal zinc-binding RING domain, forms translocation subcomplex with Pex10p and Pex12p which functions in peroxisomal matrix protein import Gene:PEX32(YBR168W)|FD-Score:-3.57|P-value:1.77E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:RAS1(YOR101W)|FD-Score:3.97|P-value:3.55E-5||SGD DESC:GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress Gene:RCY1(YJL204C)|FD-Score:8.69|P-value:1.88E-18||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RIM101(YHL027W)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RIM8(YGL045W)|FD-Score:6.58|P-value:2.42E-11||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RMD5(YDR255C)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:Component of GID Complex that confers ubiquitin ligase (U3) activity; necessary for polyubiquitination and degradation of the gluconeogenic enzyme fructose-1,6-bisphosphatase; forms dimer with Fyv10p that is then recruited to GID Complex by Gid8p; also required for sporulation; conserved protein that has a degenerate RING finger domain Gene:RNR4(YGR180C)|FD-Score:-4.83|P-value:6.83E-7||SGD DESC:Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:ROG1(YGL144C)|FD-Score:-3.17|P-value:7.67E-4||SGD DESC:Protein with putative serine active lipase domain; ROG1 has a paralog, YDL109C, that arose from the whole genome duplication Gene:ROM2(YLR371W)|FD-Score:-4.81|P-value:7.74E-7||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL19B(YBL027W)|FD-Score:3.2|P-value:6.90E-4||SGD DESC:Ribosomal 60S subunit protein L19B; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19B has a paralog, RPL19A, that arose from the whole genome duplication Gene:RPO41(YFL036W)|FD-Score:10.4|P-value:1.13E-25||SGD DESC:Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:RPS11B(YBR048W)|FD-Score:3.29|P-value:5.07E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11B has a paralog, RPS11A, that arose from the whole genome duplication Gene:SBH1(YER087C-B)|FD-Score:-4.57|P-value:2.46E-6||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SET4(YJL105W)|FD-Score:4.19|P-value:1.41E-5||SGD DESC:Protein of unknown function, contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication Gene:SHC1(YER096W)|FD-Score:3.92|P-value:4.36E-5||SGD DESC:Sporulation-specific activator of Chs3p (chitin synthase III); required for the synthesis of the chitosan layer of ascospores; transcriptionally induced at alkaline pH; SHC1 has a paralog, SKT5, that arose from the whole genome duplication Gene:SLM5(YCR024C)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Mitochondrial asparaginyl-tRNA synthetase Gene:SLZ1(YNL196C_p)|FD-Score:-3.25|P-value:5.71E-4||SGD DESC:Sporulation-specific protein with a leucine zipper motif Gene:SNF2(YOR290C)|FD-Score:-3.79|P-value:7.59E-5||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SNF8(YPL002C)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SPR6(YER115C)|FD-Score:5.91|P-value:1.71E-9||SGD DESC:Protein of unknown function, expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation Gene:SRL1(YOR247W)|FD-Score:-4.67|P-value:1.49E-6||SGD DESC:Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication Gene:SSQ1(YLR369W)|FD-Score:3.93|P-value:4.24E-5||SGD DESC:Mitochondrial hsp70-type molecular chaperone, required for assembly of iron/sulfur clusters into proteins at a step after cluster synthesis, and for maturation of Yfh1p, which is a homolog of human frataxin implicated in Friedreich's ataxia Gene:STD1(YOR047C)|FD-Score:3.17|P-value:7.55E-4||SGD DESC:Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+] Gene:STP22(YCL008C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:TDA8(YAL064C-A_p)|FD-Score:3.13|P-value:8.88E-4||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene Gene:URA2(YJL130C)|FD-Score:3.87|P-value:5.52E-5||SGD DESC:Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP Gene:URM1(YIL008W)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VID22(YLR373C)|FD-Score:4|P-value:3.12E-5||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:VID30(YGL227W)|FD-Score:5.22|P-value:8.77E-8||SGD DESC:Central component of GID Complex, involved in FBPase degradation; interacts strongly with Gid8p to serve as a scaffold for other GID Complex subunits; contains SPRY domain and 3 domains that are also found in Gid8p - LisH, CTLH, and CRA; required for association of Vid vesicles and actin patches in vacuole import and degradation pathway; shifts the balance of nitrogen metabolism toward glutamate production; localizes to the nucleus and the cytoplasm Gene:VPS25(YJR102C)|FD-Score:4.2|P-value:1.34E-5||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS28(YPL065W)|FD-Score:5.73|P-value:4.93E-9||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS5(YOR069W)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes Gene:VPS65(YLR322W_d)|FD-Score:-4.29|P-value:8.86E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth Gene:YAF9(YNL107W)|FD-Score:-4.48|P-value:3.69E-6||SGD DESC:Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain Gene:YBL059W(YBL059W_p)|FD-Score:5.58|P-value:1.18E-8||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication Gene:YBL095W(YBL095W_p)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YBR184W(YBR184W_p)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Putative protein of unknown function; YBR184W is not an essential gene Gene:YBR238C(YBR238C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YDL118W(YDL118W_p)|FD-Score:7|P-value:1.30E-12||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDR115W(YDR115W_p)|FD-Score:-3.52|P-value:2.13E-4||SGD DESC:Putative mitochondrial ribosomal protein of the large subunit; similar to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins; protein increases in abundance and relocalizes to the plasma membrane upon DNA replication stress Gene:YGL010W(YGL010W_p)|FD-Score:5.42|P-value:3.02E-8||SGD DESC:Putative protein of unknown function; YGL010W is not an essential gene Gene:YHR045W(YHR045W_p)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Putative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum Gene:YJL215C(YJL215C_d)|FD-Score:3.13|P-value:8.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJR015W(YJR015W_p)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Putative protein of unknown function; localizes to the endoplasmic reticulum and cytoplasm; predicted to encode a membrane transporter based on phylogenetic analysis; YJR015W is a non-essential gene Gene:YLL047W(YLL047W_d)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RNP1 Gene:YML012C-A(YML012C-A_d)|FD-Score:5.73|P-value:5.03E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YNL134C(YNL134C_p)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Protein of unknown function; similar to dehydrogenases from other model organisms; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress Gene:YOR072W(YOR072W_d)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPK1(YKL126W)|FD-Score:-3.3|P-value:4.80E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:ADH5(YBR145W)|FD-Score:3.85|P-value:5.84E-5||SGD DESC:Alcohol dehydrogenase isoenzyme V; involved in ethanol production; ADH5 has a paralog, ADH1, that arose from the whole genome duplication Gene:AFG1(YEL052W)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Conserved protein that may act as a chaperone in the degradation of misfolded or unassembled cytochrome c oxidase subunits; localized to matrix face of the mitochondrial inner membrane; member of the AAA family but lacks a protease domain Gene:AFT1(YGL071W)|FD-Score:-5.76|P-value:4.15E-9||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARF1(YDL192W)|FD-Score:3.2|P-value:6.86E-4||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication Gene:ARO1(YDR127W)|FD-Score:-3.23|P-value:6.15E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:-3.65|P-value:1.33E-4||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ASM4(YDL088C)|FD-Score:-6.8|P-value:5.33E-12||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication Gene:BEM2(YER155C)|FD-Score:-3.26|P-value:5.58E-4||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence Gene:BRE5(YNR051C)|FD-Score:-3.26|P-value:5.58E-4||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:CAN1(YEL063C)|FD-Score:3.19|P-value:7.01E-4||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:CAX4(YGR036C)|FD-Score:-5.59|P-value:1.14E-8||SGD DESC:Dolichyl pyrophosphate (Dol-P-P) phosphatase with a luminally oriented active site in the ER, cleaves the anhydride linkage in Dol-P-P, required for Dol-P-P-linked oligosaccharide intermediate synthesis and protein N-glycosylation Gene:CDC50(YCR094W)|FD-Score:12.5|P-value:5.77E-36||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CEM1(YER061C)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:Mitochondrial beta-keto-acyl synthase with possible role in fatty acid synthesis; required for mitochondrial respiration Gene:CTK1(YKL139W)|FD-Score:7.7|P-value:6.83E-15||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:DAP1(YPL170W)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:DRS2(YAL026C)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:ECM33(YBR078W)|FD-Score:8.36|P-value:3.21E-17||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:ERP1(YAR002C-A)|FD-Score:4.74|P-value:1.04E-6||SGD DESC:Member of the p24 family involved in ER to Golgi transport; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication Gene:FCY21(YER060W)|FD-Score:5.08|P-value:1.88E-7||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:FRE5(YOR384W)|FD-Score:3.72|P-value:9.98E-5||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRT2(YAL028W)|FD-Score:4.31|P-value:8.20E-6||SGD DESC:Tail-anchored ER membrane protein of unknown function; interacts with homolog Frt1p; promotes growth in conditions of high Na+, alkaline pH, or cell wall stress, possibly via a role in posttranslational translocation; potential Cdc28p substrate; FRT2 has a paralog, FRT1, that arose from the whole genome duplication Gene:FZO1(YBR179C)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system Gene:GCV3(YAL044C)|FD-Score:4.64|P-value:1.77E-6||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:GIT1(YCR098C)|FD-Score:3.12|P-value:8.91E-4||SGD DESC:Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability Gene:HMF1(YER057C)|FD-Score:5.05|P-value:2.18E-7||SGD DESC:Member of the p14.5 protein family with similarity to Mmf1p, functionally complements Mmf1p function when targeted to mitochondria; heat shock inducible; high-dosage growth inhibitor; forms a homotrimer in vitro Gene:HTD2(YHR067W)|FD-Score:4.89|P-value:5.03E-7||SGD DESC:Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology Gene:HUR1(YGL168W)|FD-Score:-3.21|P-value:6.73E-4||SGD DESC:Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene Gene:HVG1(YER039C)|FD-Score:4.63|P-value:1.81E-6||SGD DESC:Protein of unknown function, has homology to Vrg4p Gene:ICT1(YLR099C)|FD-Score:4.45|P-value:4.26E-6||SGD DESC:Lysophosphatidic acid acyltransferase; responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress; ICT1 has a paralog, ECM18, that arose from the whole genome duplication Gene:IMG1(YCR046C)|FD-Score:6.89|P-value:2.80E-12||SGD DESC:Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome Gene:IOC4(YMR044W)|FD-Score:4.11|P-value:2.00E-5||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing Gene:IPT1(YDR072C)|FD-Score:4.39|P-value:5.62E-6||SGD DESC:Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin Gene:JEM1(YJL073W)|FD-Score:4.82|P-value:7.25E-7||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:KCH1(YJR054W_p)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate Gene:MAC1(YMR021C)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MAL33(YBR297W)|FD-Score:3.18|P-value:7.32E-4||SGD DESC:MAL-activator protein, part of complex locus MAL3; nonfunctional in genomic reference strain S288C Gene:MCX1(YBR227C)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Mitochondrial matrix protein; putative ATP-binding chaperone with non-proteolytic function; similar to bacterial ClpX proteins Gene:MDJ1(YFL016C)|FD-Score:5.09|P-value:1.76E-7||SGD DESC:Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones Gene:MDL1(YLR188W)|FD-Score:4.89|P-value:4.94E-7||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, mediates export of peptides generated upon proteolysis of mitochondrial proteins, plays a role in the regulation of cellular resistance to oxidative stress Gene:MDY2(YOL111C)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MEF2(YJL102W)|FD-Score:3.82|P-value:6.56E-5||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MGM1(YOR211C)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Mitochondrial GTPase, present in complex with Ugo1p and Fzo1p; required for mt morphology and genome maintenance; exists as long and short form with different distributions; homolog of human OPA1 involved in autosomal dominant optic atrophy Gene:MRPL37(YBR268W)|FD-Score:-3.72|P-value:9.85E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL39(YML009C)|FD-Score:5.04|P-value:2.30E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MTM1(YGR257C)|FD-Score:5.11|P-value:1.64E-7||SGD DESC:Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor Gene:NDT80(YHR124W)|FD-Score:4.55|P-value:2.72E-6||SGD DESC:Meiosis-specific transcription factor required for exit from pachytene and for full meiotic recombination; activates middle sporulation genes; competes with Sum1p for binding to promoters containing middle sporulation elements (MSE) Gene:NGG1(YDR176W)|FD-Score:-3.47|P-value:2.65E-4||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:NKP2(YLR315W)|FD-Score:-4.92|P-value:4.34E-7||SGD DESC:Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2 Gene:NPL4(YBR170C)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; assists Cdc48p in the dislocation of misfolded, polyubiquitinated ERAD substrates that are subsequently delivered to the proteasome for degradation; also involved in the regulated destruction of resident ER membrane proteins, such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing (RUP) Gene:NPT1(YOR209C)|FD-Score:6.87|P-value:3.12E-12||SGD DESC:Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus Gene:NUP60(YAR002W)|FD-Score:-3.22|P-value:6.35E-4||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; both NUP1 and NUP60 are homologous to human NUP153 Gene:OXA1(YER154W)|FD-Score:3.95|P-value:3.85E-5||SGD DESC:Mitochondrial inner membrane insertase, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; conserved from bacteria to animals Gene:PEX2(YJL210W)|FD-Score:4.12|P-value:1.88E-5||SGD DESC:RING-finger peroxin and E3 ubiquitin ligase, peroxisomal membrane protein with a C-terminal zinc-binding RING domain, forms translocation subcomplex with Pex10p and Pex12p which functions in peroxisomal matrix protein import Gene:PEX32(YBR168W)|FD-Score:-3.57|P-value:1.77E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:RAS1(YOR101W)|FD-Score:3.97|P-value:3.55E-5||SGD DESC:GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress Gene:RCY1(YJL204C)|FD-Score:8.69|P-value:1.88E-18||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RIM101(YHL027W)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RIM8(YGL045W)|FD-Score:6.58|P-value:2.42E-11||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RMD5(YDR255C)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:Component of GID Complex that confers ubiquitin ligase (U3) activity; necessary for polyubiquitination and degradation of the gluconeogenic enzyme fructose-1,6-bisphosphatase; forms dimer with Fyv10p that is then recruited to GID Complex by Gid8p; also required for sporulation; conserved protein that has a degenerate RING finger domain Gene:RNR4(YGR180C)|FD-Score:-4.83|P-value:6.83E-7||SGD DESC:Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:ROG1(YGL144C)|FD-Score:-3.17|P-value:7.67E-4||SGD DESC:Protein with putative serine active lipase domain; ROG1 has a paralog, YDL109C, that arose from the whole genome duplication Gene:ROM2(YLR371W)|FD-Score:-4.81|P-value:7.74E-7||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL19B(YBL027W)|FD-Score:3.2|P-value:6.90E-4||SGD DESC:Ribosomal 60S subunit protein L19B; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19B has a paralog, RPL19A, that arose from the whole genome duplication Gene:RPO41(YFL036W)|FD-Score:10.4|P-value:1.13E-25||SGD DESC:Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:RPS11B(YBR048W)|FD-Score:3.29|P-value:5.07E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11B has a paralog, RPS11A, that arose from the whole genome duplication Gene:SBH1(YER087C-B)|FD-Score:-4.57|P-value:2.46E-6||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SET4(YJL105W)|FD-Score:4.19|P-value:1.41E-5||SGD DESC:Protein of unknown function, contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication Gene:SHC1(YER096W)|FD-Score:3.92|P-value:4.36E-5||SGD DESC:Sporulation-specific activator of Chs3p (chitin synthase III); required for the synthesis of the chitosan layer of ascospores; transcriptionally induced at alkaline pH; SHC1 has a paralog, SKT5, that arose from the whole genome duplication Gene:SLM5(YCR024C)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Mitochondrial asparaginyl-tRNA synthetase Gene:SLZ1(YNL196C_p)|FD-Score:-3.25|P-value:5.71E-4||SGD DESC:Sporulation-specific protein with a leucine zipper motif Gene:SNF2(YOR290C)|FD-Score:-3.79|P-value:7.59E-5||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SNF8(YPL002C)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SPR6(YER115C)|FD-Score:5.91|P-value:1.71E-9||SGD DESC:Protein of unknown function, expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation Gene:SRL1(YOR247W)|FD-Score:-4.67|P-value:1.49E-6||SGD DESC:Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication Gene:SSQ1(YLR369W)|FD-Score:3.93|P-value:4.24E-5||SGD DESC:Mitochondrial hsp70-type molecular chaperone, required for assembly of iron/sulfur clusters into proteins at a step after cluster synthesis, and for maturation of Yfh1p, which is a homolog of human frataxin implicated in Friedreich's ataxia Gene:STD1(YOR047C)|FD-Score:3.17|P-value:7.55E-4||SGD DESC:Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+] Gene:STP22(YCL008C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:TDA8(YAL064C-A_p)|FD-Score:3.13|P-value:8.88E-4||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene Gene:URA2(YJL130C)|FD-Score:3.87|P-value:5.52E-5||SGD DESC:Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP Gene:URM1(YIL008W)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VID22(YLR373C)|FD-Score:4|P-value:3.12E-5||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:VID30(YGL227W)|FD-Score:5.22|P-value:8.77E-8||SGD DESC:Central component of GID Complex, involved in FBPase degradation; interacts strongly with Gid8p to serve as a scaffold for other GID Complex subunits; contains SPRY domain and 3 domains that are also found in Gid8p - LisH, CTLH, and CRA; required for association of Vid vesicles and actin patches in vacuole import and degradation pathway; shifts the balance of nitrogen metabolism toward glutamate production; localizes to the nucleus and the cytoplasm Gene:VPS25(YJR102C)|FD-Score:4.2|P-value:1.34E-5||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS28(YPL065W)|FD-Score:5.73|P-value:4.93E-9||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS5(YOR069W)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes Gene:VPS65(YLR322W_d)|FD-Score:-4.29|P-value:8.86E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth Gene:YAF9(YNL107W)|FD-Score:-4.48|P-value:3.69E-6||SGD DESC:Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain Gene:YBL059W(YBL059W_p)|FD-Score:5.58|P-value:1.18E-8||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication Gene:YBL095W(YBL095W_p)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YBR184W(YBR184W_p)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Putative protein of unknown function; YBR184W is not an essential gene Gene:YBR238C(YBR238C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YDL118W(YDL118W_p)|FD-Score:7|P-value:1.30E-12||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDR115W(YDR115W_p)|FD-Score:-3.52|P-value:2.13E-4||SGD DESC:Putative mitochondrial ribosomal protein of the large subunit; similar to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins; protein increases in abundance and relocalizes to the plasma membrane upon DNA replication stress Gene:YGL010W(YGL010W_p)|FD-Score:5.42|P-value:3.02E-8||SGD DESC:Putative protein of unknown function; YGL010W is not an essential gene Gene:YHR045W(YHR045W_p)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Putative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum Gene:YJL215C(YJL215C_d)|FD-Score:3.13|P-value:8.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJR015W(YJR015W_p)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Putative protein of unknown function; localizes to the endoplasmic reticulum and cytoplasm; predicted to encode a membrane transporter based on phylogenetic analysis; YJR015W is a non-essential gene Gene:YLL047W(YLL047W_d)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RNP1 Gene:YML012C-A(YML012C-A_d)|FD-Score:5.73|P-value:5.03E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YNL134C(YNL134C_p)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Protein of unknown function; similar to dehydrogenases from other model organisms; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress Gene:YOR072W(YOR072W_d)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPK1(YKL126W)|FD-Score:-3.3|P-value:4.80E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YPL204W3.973.59E-50.68HRR25Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta)
YGL074C_d3.934.29E-50.68YGL074C_dDubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor
YDR047W3.836.35E-50.68HEM12Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
YER006W3.158.20E-40.11NUG1GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus
YPR034W3.040.001190.14ARP7Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YCL043C2.900.001870.10PDI1Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication
YGR216C2.790.002600.06GPI1Membrane protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1p are functional homologs
YKR068C2.730.003120.02BET3Hydrophilic protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles; component of the TRAPP (transport protein particle) complex
YHR188C2.720.003310.05GPI16Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-Tp homolog
YLR033W2.660.003870.03RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YNL267W2.630.004220.05PIK1Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
YJL050W2.580.004920.09MTR4ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing
YLR336C2.490.006400.02SGD1Essential nuclear protein, required for biogenesis of the small ribosomal subunit; has a possible role in the osmoregulatory glycerol response; putative homolog of human NOM1 which is implicated in acute myeloid leukemia
YPL251W_d2.470.006710.02YPL251W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C
YDR301W2.450.007060.02CFT1RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF

Top 15 HOM fitness defect scores (HOP)

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Deletion Strain FD score P-value Gene Gene Description
YCR094W12.505.77E-36CDC50Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication
YFL036W10.401.13E-25RPO41Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation
YJL204C8.691.88E-18RCY1F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
YBR078W8.363.21E-17ECM33GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p
YKL139W7.706.83E-15CTK1Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13
YDL118W_p7.001.30E-12YDL118W_pDubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein
YCR046C6.892.80E-12IMG1Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome
YOR209C6.873.12E-12NPT1Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus
YGL045W6.582.42E-11RIM8Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family
YER115C5.911.71E-9SPR6Protein of unknown function, expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation
YPL065W5.734.93E-9VPS28Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p
YML012C-A_d5.735.03E-9YML012C-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1
YBL059W_p5.581.18E-8YBL059W_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication
YGL010W_p5.423.02E-8YGL010W_pPutative protein of unknown function; YGL010W is not an essential gene
YGL227W5.228.77E-8VID30Central component of GID Complex, involved in FBPase degradation; interacts strongly with Gid8p to serve as a scaffold for other GID Complex subunits; contains SPRY domain and 3 domains that are also found in Gid8p - LisH, CTLH, and CRA; required for association of Vid vesicles and actin patches in vacuole import and degradation pathway; shifts the balance of nitrogen metabolism toward glutamate production; localizes to the nucleus and the cytoplasm

GO enrichment analysis for SGTC_2965
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2813.35E-107SGTC_30629077494 49.5 μMChembridge (Drug-like library)164596330.673077mitochondrial response to ROS
0.2445.52E-81SGTC_13531498-1206 8.1 μMChemDiv (Drug-like library)67568080.084507mitochondrial response to ROS
0.2392.84E-77SGTC_1410443-0274 32.3 μMChemDiv (Drug-like library)16272410.08mitochondrial response to ROS
0.2383.67E-77SGTC_5471120-0019 610.0 nMChemDiv (Drug-like library)36769390.0851064azole & statin
0.2275.39E-70SGTC_3832324-0395 7.4 μMChemDiv (Drug-like library)31464210.0823529azole & statin
0.2195.76E-65SGTC_1036k216-0215 33.5 μMChemDiv (Drug-like library)6223300.119403mitochondrial response to ROS
0.2151.26E-62SGTC_7783456-2434 196.0 μMChemDiv (Drug-like library)55390630.19403mitochondrial response to ROS
0.2144.14E-62SGTC_1160155-0182 31.8 μMChemDiv (Drug-like library)7714860.0869565mitochondrial response to ROS
0.2049.11E-57SGTC_29259010536 32.5 μMChembridge (Drug-like library)29941770.0810811azole & statin
0.1882.57E-48SGTC_13471493-0251 600.0 nMChemDiv (Drug-like library)39863780.0793651mitochondrial response to ROS
0.1825.53E-45SGTC_20545233173 129.0 μMChembridge (Fragment library)68890.131148mitochondrial response to ROS
0.1599.39E-35SGTC_12400356-0016 42.1 μMChemDiv (Drug-like library)43056130.131579mitochondrial response to ROS
0.1561.43E-33SGTC_9021120-0002 2.1 μMChemDiv (Drug-like library)32804750.0851064azole & statin
0.1521.76E-31SGTC_3720155-0182 15.9 μMChemDiv (Drug-like library)7714860.0869565mitochondrial response to ROS
0.1339.16E-25SGTC_29107948405 26.0 μMChembridge (Drug-like library)29713280.117647plasma membrane duress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3062907749449.47 μM0.67307716459633Chembridge (Drug-like library)361.190022.8624mitochondrial response to ROS
SGTC_3004907612271.43 μM0.35937544782551Chembridge (Drug-like library)267.322383.53612
SGTC_2897904859812.99 μM0.3548396467404Chembridge (Drug-like library)283.321782.99113
SGTC_2880904298358.44 μM0.3538466464714Chembridge (Drug-like library)341.4224633.18914fatty acid desaturase (OLE1)
SGTC_3282910849815.46 μM0.35294116459645Chembridge (Drug-like library)280.2947831.4215
SGTC_21505556613200 μM0.333333743190Chembridge (Fragment library)213.235241.22813
SGTC_13401486-1359257 μM0.3278694309068ChemDiv (Drug-like library)285.31950.99324
SGTC_23236148992148.28 μM0.3278692888220Chembridge (Fragment library)264.300281.55124
SGTC_20134024628200 μM0.3275865230760Chembridge (Fragment library)226.273762.35422
SGTC_2957907680011.52 μM0.32352916459615Chembridge (Drug-like library)290.357482.18614