0986-0032

2-isoquinolin-2-ium-2-yl-1-(4-phenylphenyl)propan-1-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_297
Screen concentration 24.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 2847425
SMILES CC(C(=O)C1=CC=C(C=C1)C2=CC=CC=C2)[N+]3=CC4=CC=CC=C4C=C3
Standardized SMILES CC(C(=O)c1ccc(cc1)c2ccccc2)[n+]3ccc4ccccc4c3
Molecular weight 338.4217
ALogP 5.6
H-bond donor count 0
H-bond acceptor count 1
Response signature 60S ribosome export

Pool Growth Kinetics
% growth inhibition (Het. pool) 20.76
% growth inhibition (Hom. pool) 17.72


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2847425
Download HIP data (tab-delimited text)  (excel)
Gene:ARP9(YMR033W)|FD-Score:-3.14|P-value:8.53E-4|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CAB1(YDR531W)|FD-Score:3.23|P-value:6.24E-4|Clearance:0.13||SGD DESC:Pantothenate kinase (ATP:D-pantothenate 4'-phosphotransferase, EC 2.7.1.33); catalyzes the first committed step in the universal biosynthetic pathway for synthesis of coenzyme A (CoA); transcriptionally regulated by Upc2p via a sterol response element Gene:ERG10(YPL028W)|FD-Score:3.39|P-value:3.44E-4|Clearance:0.05||SGD DESC:Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis Gene:HCA4(YJL033W)|FD-Score:3.76|P-value:8.34E-5|Clearance:0.11||SGD DESC:DEAD box RNA helicase; high-copy number suppression of a U14 snoRNA processing mutant suggests an involvement in 18S rRNA synthesis Gene:LSG1(YGL099W)|FD-Score:7.88|P-value:1.65E-15|Clearance:2.47||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:NMD3(YHR170W)|FD-Score:3.35|P-value:4.10E-4|Clearance:0.12||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOG2(YNR053C)|FD-Score:5.41|P-value:3.19E-8|Clearance:1.49||SGD DESC:Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation Gene:ORC5(YNL261W)|FD-Score:3.41|P-value:3.27E-4|Clearance:0.01||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:RPS3(YNL178W)|FD-Score:-3.4|P-value:3.41E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:SKP1(YDR328C)|FD-Score:3.92|P-value:4.48E-5|Clearance:0.15||SGD DESC:Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress Gene:SPB1(YCL054W)|FD-Score:-3.45|P-value:2.84E-4|Clearance:0||SGD DESC:AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants Gene:TAD3(YLR316C)|FD-Score:-3.17|P-value:7.67E-4|Clearance:0||SGD DESC:Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad2p that converts adenosine to inosine at the wobble position of several tRNAs Gene:TAF8(YML114C)|FD-Score:-3.16|P-value:7.87E-4|Clearance:0||SGD DESC:TFIID subunit (65 kDa), involved in RNA polymerase II transcription initiation Gene:TFB1(YDR311W)|FD-Score:3.1|P-value:9.76E-4|Clearance:0.07||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators Gene:TIF35(YDR429C)|FD-Score:-3.17|P-value:7.59E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:VRG4(YGL225W)|FD-Score:3.65|P-value:1.29E-4|Clearance:0.25||SGD DESC:Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi Gene:ARP9(YMR033W)|FD-Score:-3.14|P-value:8.53E-4|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CAB1(YDR531W)|FD-Score:3.23|P-value:6.24E-4|Clearance:0.13||SGD DESC:Pantothenate kinase (ATP:D-pantothenate 4'-phosphotransferase, EC 2.7.1.33); catalyzes the first committed step in the universal biosynthetic pathway for synthesis of coenzyme A (CoA); transcriptionally regulated by Upc2p via a sterol response element Gene:ERG10(YPL028W)|FD-Score:3.39|P-value:3.44E-4|Clearance:0.05||SGD DESC:Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis Gene:HCA4(YJL033W)|FD-Score:3.76|P-value:8.34E-5|Clearance:0.11||SGD DESC:DEAD box RNA helicase; high-copy number suppression of a U14 snoRNA processing mutant suggests an involvement in 18S rRNA synthesis Gene:LSG1(YGL099W)|FD-Score:7.88|P-value:1.65E-15|Clearance:2.47||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:NMD3(YHR170W)|FD-Score:3.35|P-value:4.10E-4|Clearance:0.12||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOG2(YNR053C)|FD-Score:5.41|P-value:3.19E-8|Clearance:1.49||SGD DESC:Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation Gene:ORC5(YNL261W)|FD-Score:3.41|P-value:3.27E-4|Clearance:0.01||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:RPS3(YNL178W)|FD-Score:-3.4|P-value:3.41E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:SKP1(YDR328C)|FD-Score:3.92|P-value:4.48E-5|Clearance:0.15||SGD DESC:Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress Gene:SPB1(YCL054W)|FD-Score:-3.45|P-value:2.84E-4|Clearance:0||SGD DESC:AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants Gene:TAD3(YLR316C)|FD-Score:-3.17|P-value:7.67E-4|Clearance:0||SGD DESC:Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad2p that converts adenosine to inosine at the wobble position of several tRNAs Gene:TAF8(YML114C)|FD-Score:-3.16|P-value:7.87E-4|Clearance:0||SGD DESC:TFIID subunit (65 kDa), involved in RNA polymerase II transcription initiation Gene:TFB1(YDR311W)|FD-Score:3.1|P-value:9.76E-4|Clearance:0.07||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators Gene:TIF35(YDR429C)|FD-Score:-3.17|P-value:7.59E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:VRG4(YGL225W)|FD-Score:3.65|P-value:1.29E-4|Clearance:0.25||SGD DESC:Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2847425
Download HOP data (tab-delimited text)  (excel)
Gene:AFT1(YGL071W)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AUS1(YOR011W)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen Gene:BSC6(YOL137W)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:Protein of unknown function containing 8 putative transmembrane seqments; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression Gene:BUB2(YMR055C)|FD-Score:3.1|P-value:9.68E-4||SGD DESC:Mitotic exit network regulator, forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage Gene:COX10(YPL172C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders Gene:CTR1(YPR124W)|FD-Score:-4.05|P-value:2.51E-5||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:DCN1(YLR128W)|FD-Score:3.17|P-value:7.70E-4||SGD DESC:Scaffold-type E3 ligase required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation Gene:DNF2(YDR093W)|FD-Score:-3.4|P-value:3.31E-4||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:EXO1(YOR033C)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:5'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair and DNA mismatch repair; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress Gene:FAA2(YER015W)|FD-Score:-3.63|P-value:1.44E-4||SGD DESC:Medium chain fatty acyl-CoA synthetase, activates imported fatty acids; accepts a wide range of fatty acid chain lengths with a preference for medium chains, C9:0-C13:0; localized to the peroxisome Gene:GAL10(YBR019C)|FD-Score:3.92|P-value:4.40E-5||SGD DESC:UDP-glucose-4-epimerase, catalyzes the interconversion of UDP-galactose and UDP-D-glucose in galactose metabolism; also catalyzes the conversion of alpha-D-glucose or alpha-D-galactose to their beta-anomers Gene:GSY2(YLR258W)|FD-Score:3.26|P-value:5.53E-4||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:HHF1(YBR009C)|FD-Score:5.02|P-value:2.59E-7||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HXK2(YGL253W)|FD-Score:-3.52|P-value:2.19E-4||SGD DESC:Hexokinase isoenzyme 2; catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene; phosphorylation/dephosphorylation at serine-14 by protein kinase Snf1p and protein phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; HXK2 has a paralog, HXK1, that arose from the whole genome duplication Gene:KAP120(YPL125W)|FD-Score:3.25|P-value:5.86E-4||SGD DESC:Karyopherin responsible for the nuclear import of ribosome maturation factor Rfp1p Gene:KIP2(YPL155C)|FD-Score:3.22|P-value:6.30E-4||SGD DESC:Kinesin-related motor protein involved in mitotic spindle positioning, stabilizes microtubules by targeting Bik1p to the plus end; Kip2p levels are controlled during the cell cycle Gene:MRT4(YKL009W)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Protein involved in mRNA turnover and ribosome assembly, localizes to the nucleolus Gene:MTF2(YDL044C)|FD-Score:3.22|P-value:6.41E-4||SGD DESC:Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription Gene:NBP2(YDR162C)|FD-Score:-3.72|P-value:9.81E-5||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:OPI3(YJR073C)|FD-Score:-3.82|P-value:6.64E-5||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:ORT1(YOR130C)|FD-Score:-3.12|P-value:8.99E-4||SGD DESC:Ornithine transporter of the mitochondrial inner membrane, exports ornithine from mitochondria as part of arginine biosynthesis; human ortholog is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome Gene:PGD1(YGL025C)|FD-Score:-4|P-value:3.17E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PUF2(YPR042C)|FD-Score:3.87|P-value:5.34E-5||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins Gene:REC104(YHR157W)|FD-Score:3.2|P-value:6.75E-4||SGD DESC:Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination Gene:REG1(YDR028C)|FD-Score:-4.09|P-value:2.17E-5||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RPN10(YHR200W)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein Gene:RRP8(YDR083W)|FD-Score:3.97|P-value:3.53E-5||SGD DESC:Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1 Gene:SGF29(YCL010C)|FD-Score:-3.53|P-value:2.05E-4||SGD DESC:Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation Gene:SSD1(YDR293C)|FD-Score:-4.3|P-value:8.35E-6||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SWS2(YNL081C)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TMA23(YMR269W)|FD-Score:4.23|P-value:1.17E-5||SGD DESC:Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan Gene:UME6(YDR207C)|FD-Score:-3.81|P-value:7.00E-5||SGD DESC:Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p Gene:VPS36(YLR417W)|FD-Score:-3.35|P-value:4.05E-4||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:YAP1802(YGR241C)|FD-Score:3.31|P-value:4.68E-4||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication Gene:YBR056W-A(YBR056W-A_p)|FD-Score:3.1|P-value:9.81E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the dubious ORF YBR056C-B Gene:YBR141C(YBR141C_p)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YBR221W-A(YBR221W-A_p)|FD-Score:4.65|P-value:1.63E-6||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YDR444W(YDR444W_p)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Putative hydrolase acting on ester bonds Gene:YDR455C(YDR455C_d)|FD-Score:-3.61|P-value:1.54E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YDR541C(YDR541C_p)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Putative dihydrokaempferol 4-reductase Gene:YER053C-A(YER053C-A_p)|FD-Score:3.92|P-value:4.45E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:YGL082W(YGL082W_p)|FD-Score:3.84|P-value:6.26E-5||SGD DESC:Putative protein of unknown function; predicted prenylation/proteolysis target of Afc1p and Rce1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YGL082W is not an essential gene Gene:YOR062C(YOR062C_p)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Protein of unknown function; similar to YKR075Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YPK1(YKL126W)|FD-Score:-5.16|P-value:1.23E-7||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR053C(YPR053C_d)|FD-Score:3.25|P-value:5.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene NHP6A/YPR052C Gene:AFT1(YGL071W)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AUS1(YOR011W)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen Gene:BSC6(YOL137W)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:Protein of unknown function containing 8 putative transmembrane seqments; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression Gene:BUB2(YMR055C)|FD-Score:3.1|P-value:9.68E-4||SGD DESC:Mitotic exit network regulator, forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage Gene:COX10(YPL172C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders Gene:CTR1(YPR124W)|FD-Score:-4.05|P-value:2.51E-5||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:DCN1(YLR128W)|FD-Score:3.17|P-value:7.70E-4||SGD DESC:Scaffold-type E3 ligase required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation Gene:DNF2(YDR093W)|FD-Score:-3.4|P-value:3.31E-4||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:EXO1(YOR033C)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:5'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair and DNA mismatch repair; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress Gene:FAA2(YER015W)|FD-Score:-3.63|P-value:1.44E-4||SGD DESC:Medium chain fatty acyl-CoA synthetase, activates imported fatty acids; accepts a wide range of fatty acid chain lengths with a preference for medium chains, C9:0-C13:0; localized to the peroxisome Gene:GAL10(YBR019C)|FD-Score:3.92|P-value:4.40E-5||SGD DESC:UDP-glucose-4-epimerase, catalyzes the interconversion of UDP-galactose and UDP-D-glucose in galactose metabolism; also catalyzes the conversion of alpha-D-glucose or alpha-D-galactose to their beta-anomers Gene:GSY2(YLR258W)|FD-Score:3.26|P-value:5.53E-4||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:HHF1(YBR009C)|FD-Score:5.02|P-value:2.59E-7||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HXK2(YGL253W)|FD-Score:-3.52|P-value:2.19E-4||SGD DESC:Hexokinase isoenzyme 2; catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene; phosphorylation/dephosphorylation at serine-14 by protein kinase Snf1p and protein phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; HXK2 has a paralog, HXK1, that arose from the whole genome duplication Gene:KAP120(YPL125W)|FD-Score:3.25|P-value:5.86E-4||SGD DESC:Karyopherin responsible for the nuclear import of ribosome maturation factor Rfp1p Gene:KIP2(YPL155C)|FD-Score:3.22|P-value:6.30E-4||SGD DESC:Kinesin-related motor protein involved in mitotic spindle positioning, stabilizes microtubules by targeting Bik1p to the plus end; Kip2p levels are controlled during the cell cycle Gene:MRT4(YKL009W)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Protein involved in mRNA turnover and ribosome assembly, localizes to the nucleolus Gene:MTF2(YDL044C)|FD-Score:3.22|P-value:6.41E-4||SGD DESC:Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription Gene:NBP2(YDR162C)|FD-Score:-3.72|P-value:9.81E-5||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:OPI3(YJR073C)|FD-Score:-3.82|P-value:6.64E-5||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:ORT1(YOR130C)|FD-Score:-3.12|P-value:8.99E-4||SGD DESC:Ornithine transporter of the mitochondrial inner membrane, exports ornithine from mitochondria as part of arginine biosynthesis; human ortholog is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome Gene:PGD1(YGL025C)|FD-Score:-4|P-value:3.17E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PUF2(YPR042C)|FD-Score:3.87|P-value:5.34E-5||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins Gene:REC104(YHR157W)|FD-Score:3.2|P-value:6.75E-4||SGD DESC:Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination Gene:REG1(YDR028C)|FD-Score:-4.09|P-value:2.17E-5||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RPN10(YHR200W)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein Gene:RRP8(YDR083W)|FD-Score:3.97|P-value:3.53E-5||SGD DESC:Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1 Gene:SGF29(YCL010C)|FD-Score:-3.53|P-value:2.05E-4||SGD DESC:Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation Gene:SSD1(YDR293C)|FD-Score:-4.3|P-value:8.35E-6||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SWS2(YNL081C)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TMA23(YMR269W)|FD-Score:4.23|P-value:1.17E-5||SGD DESC:Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan Gene:UME6(YDR207C)|FD-Score:-3.81|P-value:7.00E-5||SGD DESC:Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p Gene:VPS36(YLR417W)|FD-Score:-3.35|P-value:4.05E-4||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:YAP1802(YGR241C)|FD-Score:3.31|P-value:4.68E-4||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication Gene:YBR056W-A(YBR056W-A_p)|FD-Score:3.1|P-value:9.81E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the dubious ORF YBR056C-B Gene:YBR141C(YBR141C_p)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YBR221W-A(YBR221W-A_p)|FD-Score:4.65|P-value:1.63E-6||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YDR444W(YDR444W_p)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Putative hydrolase acting on ester bonds Gene:YDR455C(YDR455C_d)|FD-Score:-3.61|P-value:1.54E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YDR541C(YDR541C_p)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Putative dihydrokaempferol 4-reductase Gene:YER053C-A(YER053C-A_p)|FD-Score:3.92|P-value:4.45E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:YGL082W(YGL082W_p)|FD-Score:3.84|P-value:6.26E-5||SGD DESC:Putative protein of unknown function; predicted prenylation/proteolysis target of Afc1p and Rce1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YGL082W is not an essential gene Gene:YOR062C(YOR062C_p)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Protein of unknown function; similar to YKR075Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YPK1(YKL126W)|FD-Score:-5.16|P-value:1.23E-7||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR053C(YPR053C_d)|FD-Score:3.25|P-value:5.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene NHP6A/YPR052C

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL099W7.881.65E-152.47LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YNR053C5.413.19E-81.49NOG2Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation
YDR328C3.924.48E-50.15SKP1Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress
YJL033W3.768.34E-50.11HCA4DEAD box RNA helicase; high-copy number suppression of a U14 snoRNA processing mutant suggests an involvement in 18S rRNA synthesis
YGL225W3.651.29E-40.24VRG4Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi
YNL261W3.413.27E-40.01ORC5Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing
YPL028W3.393.44E-40.05ERG10Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis
YHR170W3.354.10E-40.12NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YDR531W3.236.24E-40.13CAB1Pantothenate kinase (ATP:D-pantothenate 4'-phosphotransferase, EC 2.7.1.33); catalyzes the first committed step in the universal biosynthetic pathway for synthesis of coenzyme A (CoA); transcriptionally regulated by Upc2p via a sterol response element
YDR311W3.109.76E-40.07TFB1Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators
YOR116C3.030.001230.07RPO31RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
YKL173W2.950.001570.04SNU114GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2
YPR048W2.920.001780.06TAH18Conserved NAPDH-dependent diflavin reductase; component of an early step in the cytosolic Fe-S protein assembly (CIA) machinery; transfers electrons from NADPH to the Fe-S cluster of Dre2p; plays a pro-death role under oxidative stress; Tah18p-dependent nitric oxide synthesis confers high-temperature stress tolerance
YML010W2.860.002130.06SPT5Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair
YMR197C2.800.002560.05VTI1Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR009C5.022.59E-7HHF1Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity
YBR221W-A_p4.651.63E-6YBR221W-A_pPutative protein of unknown function; identified by expression profiling and mass spectrometry
YMR269W4.231.17E-5TMA23Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan
YDR083W3.973.53E-5RRP8Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1
YBR019C3.924.40E-5GAL10UDP-glucose-4-epimerase, catalyzes the interconversion of UDP-galactose and UDP-D-glucose in galactose metabolism; also catalyzes the conversion of alpha-D-glucose or alpha-D-galactose to their beta-anomers
YER053C-A_p3.924.45E-5YER053C-A_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
YPR042C3.875.34E-5PUF2Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins
YGL082W_p3.846.26E-5YGL082W_pPutative protein of unknown function; predicted prenylation/proteolysis target of Afc1p and Rce1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YGL082W is not an essential gene
YPL172C3.671.23E-4COX10Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders
YDR444W_p3.571.77E-4YDR444W_pPutative hydrolase acting on ester bonds
YOR062C_p3.561.88E-4YOR062C_pProtein of unknown function; similar to YKR075Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS
YBR141C_p3.551.91E-4YBR141C_pPutative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene
YDR541C_p3.551.91E-4YDR541C_pPutative dihydrokaempferol 4-reductase
YKL009W3.542.02E-4MRT4Protein involved in mRNA turnover and ribosome assembly, localizes to the nucleolus
YOR011W3.492.45E-4AUS1Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen

GO enrichment analysis for SGTC_297
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2228.38E-67SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.089552260S ribosome export
0.2201.29E-65SGTC_8103825-7150 143.0 μMChemDiv (Drug-like library)223304110.094594660S ribosome export
0.1971.73E-52SGTC_1958st076513 41.9 μMTimTec (Natural product derivative library)7321220.092307760S ribosome export
0.1887.14E-48SGTC_1775st081588 35.2 μMTimTec (Natural product derivative library)14264430.089743660S ribosome export
0.1843.29E-46SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.13414660S ribosome export
0.1692.91E-39SGTC_1829berberine 14.4 μMTimTec (Natural product derivative library)23530.094594660S ribosome export
0.1673.19E-38SGTC_970535-0701 19.1 μMChemDiv (Drug-like library)28320130.08450760S ribosome export
0.1666.42E-38SGTC_490717-0965 32.1 μMChemDiv (Drug-like library)58893490.13235360S ribosome export
0.1575.10E-34SGTC_1350933-0004 26.3 μMChemDiv (Drug-like library)212306170.088235360S ribosome export
0.1511.36E-31SGTC_1827berberine 7.2 μMTimTec (Natural product derivative library)6294130.094594660S ribosome export
0.1497.88E-31SGTC_1070indatraline 8.8 μMNIH Clinical Collection103144720.096774260S ribosome export
0.1411.03E-27SGTC_1664st014217 9.0 μMTimTec (Natural product derivative library)39827820.07560S ribosome export
0.1321.63E-24SGTC_28097964133 13.0 μMChembridge (Drug-like library)12536650.1260S ribosome export
0.1317.02E-24SGTC_22067254556 147.0 μMChembridge (Fragment library)43989050.12698460S ribosome export
0.1315.98E-24SGTC_31709103054 49.5 μMChembridge (Drug-like library)164329700.13043560S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_6090986-002686.3 μM0.6666672848610ChemDiv (Drug-like library)302.38965.17901calcium & mitochondrial duress
SGTC_6010986-002962.9 μM0.65116324747981ChemDiv (Drug-like library)367.259085.44101calcium & mitochondrial duress
SGTC_330986-003335.82 μM0.5833332848082ChemDiv (Drug-like library)407.483725.47712calcium & mitochondrial duress
SGTC_400327-010319.75 μM0.511111409036ChemDiv (Drug-like library)324.395125.11701
SGTC_680986-028482.83 μM0.387755423278ChemDiv (Drug-like library)324.395125.40801
SGTC_15091326-113180 μM0.355556768313ChemDiv (Drug-like library)239.312283.09402
SGTC_390986-028358.35 μM0.3214293110600ChemDiv (Drug-like library)393.457144.99312calcium & mitochondrial duress
SGTC_2830987-007911.7 μM0.307692411556ChemDiv (Drug-like library)298.357844.50701
SGTC_230MPP+141.67 μM0.339484Miscellaneous170.230382.95300
SGTC_9272425-1185123 μM0.294118730375ChemDiv (Drug-like library)316.353243.13522