9092186

3,6-dichloro-N-(1-methylpiperidin-4-yl)-1-benzothiophene-2-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2971
Screen concentration 9.9 μM
Source Chembridge (Drug-like library)
PubChem CID 25236400
SMILES CN1CCC(CC1)NC(=O)C2=C(C3=C(S2)C=C(C=C3)Cl)Cl
Standardized SMILES CN1CCC(CC1)NC(=O)c2sc3cc(Cl)ccc3c2Cl
Molecular weight 343.2713
ALogP 3.63
H-bond donor count 1
H-bond acceptor count 3
Response signature ergosterol biosynthesis

Pool Growth Kinetics
% growth inhibition (Het. pool) 11.59
% growth inhibition (Hom. pool) 5.33


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 25236400
Download HIP data (tab-delimited text)  (excel)
Gene:BCD1(YHR040W)|FD-Score:-3.22|P-value:6.43E-4|Clearance:0||SGD DESC:Essential protein required for the accumulation of box C/D snoRNA Gene:CCL1(YPR025C)|FD-Score:-4.31|P-value:8.19E-6|Clearance:0||SGD DESC:Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters Gene:CDC43(YGL155W)|FD-Score:-3.09|P-value:9.88E-4|Clearance:0||SGD DESC:Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis Gene:DAD1(YDR016C)|FD-Score:3.51|P-value:2.25E-4|Clearance:0.12||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:DAD2(YKR083C)|FD-Score:-5.42|P-value:2.98E-8|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:EFB1(YAL003W)|FD-Score:3.15|P-value:8.25E-4|Clearance:0.01||SGD DESC:Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site Gene:ERG11(YHR007C)|FD-Score:8.39|P-value:2.34E-17|Clearance:1.92||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG7(YHR072W)|FD-Score:9.97|P-value:9.84E-24|Clearance:1.92||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:GAB1(YLR459W)|FD-Score:-3.8|P-value:7.36E-5|Clearance:0||SGD DESC:GPI transamidase subunit, involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or of the lipid portion of GPI Gene:GCD10(YNL062C)|FD-Score:-4.36|P-value:6.59E-6|Clearance:0||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:GDI1(YER136W)|FD-Score:-3.21|P-value:6.61E-4|Clearance:0||SGD DESC:GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins Gene:HSH49(YOR319W)|FD-Score:4.45|P-value:4.20E-6|Clearance:0.24||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:IPL1(YPL209C)|FD-Score:4.91|P-value:4.60E-7|Clearance:0.03||SGD DESC:Aurora kinase subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; required for SPB cohesion and prevention of multipolar spindle formation; localizes to nuclear foci that become diffuse upon DNA replication stress; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon SAC arrest Gene:KIN28(YDL108W)|FD-Score:-3.11|P-value:9.33E-4|Clearance:0||SGD DESC:Serine/threonine protein kinase, subunit of the transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters Gene:LSM5(YER146W)|FD-Score:3.7|P-value:1.08E-4|Clearance:0.02||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MTR4(YJL050W)|FD-Score:3.68|P-value:1.15E-4|Clearance:0.01||SGD DESC:ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing Gene:NSP1(YJL041W)|FD-Score:-5.29|P-value:6.17E-8|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p); forms foci at the nuclear periphery upon DNA replication stress Gene:NUP159(YIL115C)|FD-Score:4.67|P-value:1.53E-6|Clearance:0.21||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p) Gene:PIS1(YPR113W)|FD-Score:3.38|P-value:3.56E-4|Clearance:0.21||SGD DESC:Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins Gene:POP6(YGR030C)|FD-Score:3.12|P-value:9.16E-4|Clearance:0.04||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PUP1(YOR157C)|FD-Score:4.88|P-value:5.40E-7|Clearance:0.21||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:PUP2(YGR253C)|FD-Score:-3.59|P-value:1.64E-4|Clearance:0||SGD DESC:Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta Gene:RRP17(YDR412W)|FD-Score:-3.38|P-value:3.64E-4|Clearance:0||SGD DESC:Component of the pre-60S pre-ribosomal particle; required for cell viability under standard (aerobic) conditions but not under anaerobic conditions; exonuclease required for 5′ end processing of pre-60S ribosomal RNA Gene:RSC58(YLR033W)|FD-Score:6.87|P-value:3.16E-12|Clearance:1.92||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC8(YFR037C)|FD-Score:3.68|P-value:1.18E-4|Clearance:0.17||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:4.21|P-value:1.25E-5|Clearance:0.52||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SAM50(YNL026W)|FD-Score:-3.29|P-value:4.95E-4|Clearance:0||SGD DESC:Essential component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85 Gene:SFH1(YLR321C)|FD-Score:4.95|P-value:3.72E-7|Clearance:0.04||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SFT1(YKL006C-A)|FD-Score:-3.93|P-value:4.19E-5|Clearance:0||SGD DESC:Intra-Golgi v-SNARE, required for transport of proteins between an early and a later Golgi compartment Gene:SUB2(YDL084W)|FD-Score:-4.44|P-value:4.42E-6|Clearance:0||SGD DESC:Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:TUB1(YML085C)|FD-Score:3.14|P-value:8.54E-4|Clearance:0.02||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:YHC1(YLR298C)|FD-Score:3.17|P-value:7.52E-4|Clearance:0.03||SGD DESC:Component of the U1 snRNP complex required for pre-mRNA splicing; putative ortholog of human U1C protein, which is involved in formation of a complex between U1 snRNP and the pre-mRNA 5' splice site Gene:YJL195C(YJL195C_d)|FD-Score:-6.88|P-value:2.93E-12|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:BCD1(YHR040W)|FD-Score:-3.22|P-value:6.43E-4|Clearance:0||SGD DESC:Essential protein required for the accumulation of box C/D snoRNA Gene:CCL1(YPR025C)|FD-Score:-4.31|P-value:8.19E-6|Clearance:0||SGD DESC:Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters Gene:CDC43(YGL155W)|FD-Score:-3.09|P-value:9.88E-4|Clearance:0||SGD DESC:Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis Gene:DAD1(YDR016C)|FD-Score:3.51|P-value:2.25E-4|Clearance:0.12||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:DAD2(YKR083C)|FD-Score:-5.42|P-value:2.98E-8|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:EFB1(YAL003W)|FD-Score:3.15|P-value:8.25E-4|Clearance:0.01||SGD DESC:Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site Gene:ERG11(YHR007C)|FD-Score:8.39|P-value:2.34E-17|Clearance:1.92||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG7(YHR072W)|FD-Score:9.97|P-value:9.84E-24|Clearance:1.92||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:GAB1(YLR459W)|FD-Score:-3.8|P-value:7.36E-5|Clearance:0||SGD DESC:GPI transamidase subunit, involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or of the lipid portion of GPI Gene:GCD10(YNL062C)|FD-Score:-4.36|P-value:6.59E-6|Clearance:0||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:GDI1(YER136W)|FD-Score:-3.21|P-value:6.61E-4|Clearance:0||SGD DESC:GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins Gene:HSH49(YOR319W)|FD-Score:4.45|P-value:4.20E-6|Clearance:0.24||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:IPL1(YPL209C)|FD-Score:4.91|P-value:4.60E-7|Clearance:0.03||SGD DESC:Aurora kinase subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; required for SPB cohesion and prevention of multipolar spindle formation; localizes to nuclear foci that become diffuse upon DNA replication stress; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon SAC arrest Gene:KIN28(YDL108W)|FD-Score:-3.11|P-value:9.33E-4|Clearance:0||SGD DESC:Serine/threonine protein kinase, subunit of the transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters Gene:LSM5(YER146W)|FD-Score:3.7|P-value:1.08E-4|Clearance:0.02||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MTR4(YJL050W)|FD-Score:3.68|P-value:1.15E-4|Clearance:0.01||SGD DESC:ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing Gene:NSP1(YJL041W)|FD-Score:-5.29|P-value:6.17E-8|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p); forms foci at the nuclear periphery upon DNA replication stress Gene:NUP159(YIL115C)|FD-Score:4.67|P-value:1.53E-6|Clearance:0.21||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p) Gene:PIS1(YPR113W)|FD-Score:3.38|P-value:3.56E-4|Clearance:0.21||SGD DESC:Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins Gene:POP6(YGR030C)|FD-Score:3.12|P-value:9.16E-4|Clearance:0.04||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PUP1(YOR157C)|FD-Score:4.88|P-value:5.40E-7|Clearance:0.21||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:PUP2(YGR253C)|FD-Score:-3.59|P-value:1.64E-4|Clearance:0||SGD DESC:Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta Gene:RRP17(YDR412W)|FD-Score:-3.38|P-value:3.64E-4|Clearance:0||SGD DESC:Component of the pre-60S pre-ribosomal particle; required for cell viability under standard (aerobic) conditions but not under anaerobic conditions; exonuclease required for 5′ end processing of pre-60S ribosomal RNA Gene:RSC58(YLR033W)|FD-Score:6.87|P-value:3.16E-12|Clearance:1.92||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC8(YFR037C)|FD-Score:3.68|P-value:1.18E-4|Clearance:0.17||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:4.21|P-value:1.25E-5|Clearance:0.52||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SAM50(YNL026W)|FD-Score:-3.29|P-value:4.95E-4|Clearance:0||SGD DESC:Essential component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85 Gene:SFH1(YLR321C)|FD-Score:4.95|P-value:3.72E-7|Clearance:0.04||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SFT1(YKL006C-A)|FD-Score:-3.93|P-value:4.19E-5|Clearance:0||SGD DESC:Intra-Golgi v-SNARE, required for transport of proteins between an early and a later Golgi compartment Gene:SUB2(YDL084W)|FD-Score:-4.44|P-value:4.42E-6|Clearance:0||SGD DESC:Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:TUB1(YML085C)|FD-Score:3.14|P-value:8.54E-4|Clearance:0.02||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:YHC1(YLR298C)|FD-Score:3.17|P-value:7.52E-4|Clearance:0.03||SGD DESC:Component of the U1 snRNP complex required for pre-mRNA splicing; putative ortholog of human U1C protein, which is involved in formation of a complex between U1 snRNP and the pre-mRNA 5' splice site Gene:YJL195C(YJL195C_d)|FD-Score:-6.88|P-value:2.93E-12|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 25236400
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:-3.82|P-value:6.67E-5||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ACS1(YAL054C)|FD-Score:-6.82|P-value:4.68E-12||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs2p, is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions Gene:AEP3(YPL005W)|FD-Score:-4|P-value:3.22E-5||SGD DESC:Protein that may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; localized to the matrix face of the mitochondrial inner membrane; stabilizes the bicistronic AAP1-ATP6 mRNA Gene:AFG3(YER017C)|FD-Score:3.23|P-value:6.11E-4||SGD DESC:Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging Gene:AIM44(YPL158C)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:AKR2(YOR034C)|FD-Score:-4.43|P-value:4.64E-6||SGD DESC:Ankyrin repeat-containing protein; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p; AKR2 has a paralog, AKR1, that arose from the whole genome duplication Gene:APJ1(YNL077W)|FD-Score:4.69|P-value:1.38E-6||SGD DESC:Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:ARO2(YGL148W)|FD-Score:3.78|P-value:7.95E-5||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ARP1(YHR129C)|FD-Score:3.13|P-value:8.88E-4||SGD DESC:Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin Gene:BIO3(YNR058W)|FD-Score:-3.34|P-value:4.20E-4||SGD DESC:7,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:CBC2(YPL178W)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:Small subunit of the heterodimeric cap binding complex that also contains Sto1p, component of the spliceosomal commitment complex; interacts with Npl3p, possibly to package mRNA for export from the nucleus; contains an RNA-binding motif Gene:CBT1(YKL208W)|FD-Score:4.6|P-value:2.15E-6||SGD DESC:Protein involved in 5' end processing of mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p Gene:CCZ1(YBR131W)|FD-Score:6.71|P-value:9.63E-12||SGD DESC:Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway Gene:CLB6(YGR109C)|FD-Score:-3.16|P-value:7.85E-4||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 Gene:CMC1(YKL137W)|FD-Score:-3.84|P-value:6.16E-5||SGD DESC:Evolutionarily conserved copper-binding protein of the mitochondrial intermembrane space, may be involved in delivering copper from the matrix to the cytochrome c oxidase complex; contains a twin CX9C motif Gene:COG5(YNL051W)|FD-Score:-3.27|P-value:5.45E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX12(YLR038C)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Subunit VIb of cytochrome c oxidase; cytochrome c oxidase is also known as respiratory Complex IV and is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of cytochrome c oxidase but not required for activity after assembly; phosphorylated; easily released from the intermembrane space, suggesting a loose association with Complex IV Gene:COX14(YML129C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes Gene:DAL1(YIR027C)|FD-Score:6.09|P-value:5.58E-10||SGD DESC:Allantoinase, converts allantoin to allantoate in the first step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DAL7(YIR031C)|FD-Score:3.13|P-value:8.69E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DOA1(YKL213C)|FD-Score:-3.36|P-value:3.90E-4||SGD DESC:WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress Gene:DYN3(YMR299C)|FD-Score:5.14|P-value:1.34E-7||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:ECM19(YLR390W)|FD-Score:5.71|P-value:5.69E-9||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:ELO1(YJL196C)|FD-Score:-4.98|P-value:3.15E-7||SGD DESC:Elongase I, medium-chain acyl elongase; catalyzes carboxy-terminal elongation of unsaturated C12-C16 fatty acyl-CoAs to C16-C18 fatty acids; ELO1 has a paralog, FEN1, that arose from the whole genome duplication Gene:EMI2(YDR516C)|FD-Score:-3.94|P-value:4.03E-5||SGD DESC:Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:EPS1(YIL005W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family Gene:ERG28(YER044C)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:Endoplasmic reticulum membrane protein, may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p Gene:EXO1(YOR033C)|FD-Score:4.02|P-value:2.91E-5||SGD DESC:5'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair and DNA mismatch repair; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress Gene:FDC1(YDR539W)|FD-Score:-5.54|P-value:1.55E-8||SGD DESC:Putative phenylacrylic acid decarboxylase; essential for the decarboxylation of aromatic carboxylic acids to the corresponding vinyl derivatives; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm Gene:FPR3(YML074C)|FD-Score:-4.74|P-value:1.09E-6||SGD DESC:Nucleolar peptidyl-prolyl cis-trans isomerase (PPIase); FK506 binding protein; phosphorylated by casein kinase II (Cka1p-Cka2p-Ckb1p-Ckb2p) and dephosphorylated by Ptp1p Gene:GEM1(YAL048C)|FD-Score:-3.19|P-value:7.11E-4||SGD DESC:Outer mitochondrial membrane GTPase, subunit of the ERMES complex; potential regulatory subunit of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; cells lacking Gem1p contain collapsed, globular, or grape-like mitochondria; ortholog of metazoan Miro GTPases Gene:GET4(YOR164C)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Mdy2p; highly conserved across species and homologous to human gene C7orf20 Gene:GPM3(YOL056W)|FD-Score:3.19|P-value:7.03E-4||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:GSY2(YLR258W)|FD-Score:9.12|P-value:3.78E-20||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:GTF1(YGR102C)|FD-Score:4.05|P-value:2.58E-5||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; transposon insertion mutant is salt sensitive and null mutant has growth defects; non-tagged protein is detected in purified mitochondria Gene:HMG1(YML075C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress Gene:HNT3(YOR258W)|FD-Score:-4.62|P-value:1.93E-6||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:HSM3(YBR272C)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation Gene:HST2(YPL015C)|FD-Score:-3.48|P-value:2.48E-4||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:HXT17(YNR072W)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Hexose transporter, up-regulated in media containing raffinose and galactose at pH 7.7 versus pH 4.7, repressed by high levels of glucose Gene:IGO1(YNL157W)|FD-Score:-3.17|P-value:7.60E-4||SGD DESC:Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo2p Gene:INP2(YMR163C)|FD-Score:-3.39|P-value:3.46E-4||SGD DESC:Peroxisome-specific receptor important for peroxisome inheritance; co-fractionates with peroxisome membranes and co-localizes with peroxisomes in vivo; physically interacts with the myosin V motor Myo2p; INP2 is not an essential gene Gene:ISY1(YJR050W)|FD-Score:4.46|P-value:4.13E-6||SGD DESC:Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles Gene:KDX1(YKL161C)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p Gene:LYS14(YDR034C)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Transcriptional activator involved in regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer Gene:MEU1(YLR017W)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Methylthioadenosine phosphorylase (MTAP), catalyzes the initial step in the methionine salvage pathway; affects polyamine biosynthesis through regulation of ornithine decarboxylase (Spe1p) activity; regulates ADH2 gene expression Gene:MND2(YIR025W)|FD-Score:4.15|P-value:1.68E-5||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MNN11(YJL183W)|FD-Score:-3.8|P-value:7.15E-5||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MRPL11(YDL202W)|FD-Score:3.77|P-value:8.12E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NIS1(YNL078W)|FD-Score:-3.16|P-value:7.85E-4||SGD DESC:Protein localized in the bud neck at G2/M phase; physically interacts with septins; possibly involved in a mitotic signaling network Gene:NVJ1(YHR195W)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) Gene:PDR8(YLR266C)|FD-Score:4.63|P-value:1.84E-6||SGD DESC:Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication Gene:PFD1(YJL179W)|FD-Score:4.12|P-value:1.88E-5||SGD DESC:Subunit of heterohexameric prefoldin, which binds cytosolic chaperonin and transfers target proteins to it; involved in the biogenesis of actin and of alpha- and gamma-tubulin Gene:PMS1(YNL082W)|FD-Score:-3.14|P-value:8.56E-4||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:PRM4(YPL156C)|FD-Score:-3.18|P-value:7.31E-4||SGD DESC:Pheromone-regulated protein proposed to be involved in mating; predicted to have 1 transmembrane segment; transcriptionally regulated by Ste12p during mating and by Cat8p during the diauxic shift Gene:RAD2(YGR258C)|FD-Score:3.16|P-value:7.77E-4||SGD DESC:Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein Gene:RAD59(YDL059C)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p Gene:RIM13(YMR154C)|FD-Score:8.64|P-value:2.86E-18||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RLM1(YPL089C)|FD-Score:-4.73|P-value:1.13E-6||SGD DESC:MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication Gene:ROX1(YPR065W)|FD-Score:-3.22|P-value:6.41E-4||SGD DESC:Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance Gene:SAC3(YDR159W)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SIP3(YNL257C)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication Gene:SLX8(YER116C)|FD-Score:-4.11|P-value:1.95E-5||SGD DESC:Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress Gene:SNF5(YBR289W)|FD-Score:-4.3|P-value:8.70E-6||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions Gene:SRC1(YML034W)|FD-Score:5.74|P-value:4.80E-9||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:STI1(YOR027W)|FD-Score:-4.85|P-value:6.19E-7||SGD DESC:Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop Gene:TCB3(YML072C)|FD-Score:4.17|P-value:1.49E-5||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:TDA7(YNL176C_p)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Cell cycle-regulated gene of unknown function, promoter bound by Fkh2p; null mutant is sensitive to expression of the top1-T722A allele Gene:TRF5(YNL299W)|FD-Score:-4.78|P-value:8.65E-7||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p Gene:TRP3(YKL211C)|FD-Score:6.46|P-value:5.11E-11||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TVP18(YMR071C)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:UFD4(YKL010C)|FD-Score:-4.71|P-value:1.24E-6||SGD DESC:Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress Gene:URA2(YJL130C)|FD-Score:4.75|P-value:1.02E-6||SGD DESC:Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP Gene:UTR1(YJR049C)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VPS45(YGL095C)|FD-Score:-5.53|P-value:1.56E-8||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS63(YLR261C_d)|FD-Score:-3.26|P-value:5.49E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:XDJ1(YLR090W)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Chaperone with a role in facilitating mitochondrial protein import; ascomycete-specific member of the DnaJ-like family, closely related to Ydj1p; predicted to be C-terminally prenylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YBL012C(YBL012C_d)|FD-Score:4.94|P-value:3.94E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCL075W(YCL075W)|FD-Score:4.01|P-value:3.06E-5||SGD DESC:Pseudogene: encodes fragment of Ty Pol protein Gene:YDJ1(YNL064C)|FD-Score:-4.54|P-value:2.88E-6||SGD DESC:Type I HSP40 co-chaperone; involved in regulation of HSP90 and HSP70 functions; critical for determining cell size at Start as a function of growth rate; involved in protein translocation across membranes; member of the DnaJ family Gene:YDR444W(YDR444W_p)|FD-Score:-4.15|P-value:1.63E-5||SGD DESC:Putative hydrolase acting on ester bonds Gene:YEL067C(YEL067C_p)|FD-Score:-3.44|P-value:2.90E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YET3(YDL072C)|FD-Score:7.68|P-value:8.06E-15||SGD DESC:Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress Gene:YGL041C-B(YGL041C-B_p)|FD-Score:-4.5|P-value:3.41E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YHL008C(YHL008C_p)|FD-Score:6.02|P-value:8.52E-10||SGD DESC:Putative protein of unknown function, may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YHL015W-A(YHL015W-A_p)|FD-Score:4.37|P-value:6.29E-6||SGD DESC:Putative protein of unknown function Gene:YHR140W(YHR140W_p)|FD-Score:3.95|P-value:3.93E-5||SGD DESC:Putative integral membrane protein of unknown function Gene:YIL102C(YIL102C_p)|FD-Score:-3.75|P-value:8.81E-5||SGD DESC:Putative protein of unknown function Gene:YIL161W(YIL161W_p)|FD-Score:-4.2|P-value:1.31E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; mRNA is enriched in Scp160p-associated mRNPs; YIL161W is a non-essential gene Gene:YKL077W(YKL077W_p)|FD-Score:4.84|P-value:6.49E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YKL097C(YKL097C_d)|FD-Score:-3.43|P-value:3.04E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species Gene:YKR070W(YKR070W_p)|FD-Score:3.9|P-value:4.82E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLR202C(YLR202C_d)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron Gene:YLR279W(YLR279W_d)|FD-Score:4.23|P-value:1.17E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR349W(YLR349W_d)|FD-Score:-3.21|P-value:6.54E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified ORF DIC1/YLR348C Gene:YLR419W(YLR419W_p)|FD-Score:-4.59|P-value:2.18E-6||SGD DESC:Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene Gene:YML057C-A(YML057C-A_d)|FD-Score:-4.59|P-value:2.22E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene CMP2/YML057W Gene:YMR182W-A(YMR182W-A_p)|FD-Score:-4.43|P-value:4.61E-6||SGD DESC:Putative protein of unknown function Gene:YMR279C(YMR279C)|FD-Score:4.85|P-value:6.29E-7||SGD DESC:Putative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; YMR279C has a paralog, ATR1, that arose from the whole genome duplication Gene:YNL050C(YNL050C_p)|FD-Score:4.02|P-value:2.92E-5||SGD DESC:Putative protein of unknown function; YNL050c is not an essential gene Gene:YOR314W(YOR314W_d)|FD-Score:4.07|P-value:2.34E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL062W(YPL062W_d)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation Gene:YPL162C(YPL162C_p)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology Gene:YPL199C(YPL199C_p)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Putative protein of unknown function, predicted to be palmitoylated Gene:YPQ1(YOL092W_p)|FD-Score:3.16|P-value:7.93E-4||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:YPR014C(YPR014C_d)|FD-Score:5.72|P-value:5.45E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene Gene:YPR077C(YPR077C_d)|FD-Score:3.84|P-value:6.19E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1 Gene:ACB1(YGR037C)|FD-Score:-3.82|P-value:6.67E-5||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ACS1(YAL054C)|FD-Score:-6.82|P-value:4.68E-12||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs2p, is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions Gene:AEP3(YPL005W)|FD-Score:-4|P-value:3.22E-5||SGD DESC:Protein that may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; localized to the matrix face of the mitochondrial inner membrane; stabilizes the bicistronic AAP1-ATP6 mRNA Gene:AFG3(YER017C)|FD-Score:3.23|P-value:6.11E-4||SGD DESC:Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging Gene:AIM44(YPL158C)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:AKR2(YOR034C)|FD-Score:-4.43|P-value:4.64E-6||SGD DESC:Ankyrin repeat-containing protein; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p; AKR2 has a paralog, AKR1, that arose from the whole genome duplication Gene:APJ1(YNL077W)|FD-Score:4.69|P-value:1.38E-6||SGD DESC:Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:ARO2(YGL148W)|FD-Score:3.78|P-value:7.95E-5||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ARP1(YHR129C)|FD-Score:3.13|P-value:8.88E-4||SGD DESC:Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin Gene:BIO3(YNR058W)|FD-Score:-3.34|P-value:4.20E-4||SGD DESC:7,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:CBC2(YPL178W)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:Small subunit of the heterodimeric cap binding complex that also contains Sto1p, component of the spliceosomal commitment complex; interacts with Npl3p, possibly to package mRNA for export from the nucleus; contains an RNA-binding motif Gene:CBT1(YKL208W)|FD-Score:4.6|P-value:2.15E-6||SGD DESC:Protein involved in 5' end processing of mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p Gene:CCZ1(YBR131W)|FD-Score:6.71|P-value:9.63E-12||SGD DESC:Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway Gene:CLB6(YGR109C)|FD-Score:-3.16|P-value:7.85E-4||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 Gene:CMC1(YKL137W)|FD-Score:-3.84|P-value:6.16E-5||SGD DESC:Evolutionarily conserved copper-binding protein of the mitochondrial intermembrane space, may be involved in delivering copper from the matrix to the cytochrome c oxidase complex; contains a twin CX9C motif Gene:COG5(YNL051W)|FD-Score:-3.27|P-value:5.45E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX12(YLR038C)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Subunit VIb of cytochrome c oxidase; cytochrome c oxidase is also known as respiratory Complex IV and is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of cytochrome c oxidase but not required for activity after assembly; phosphorylated; easily released from the intermembrane space, suggesting a loose association with Complex IV Gene:COX14(YML129C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes Gene:DAL1(YIR027C)|FD-Score:6.09|P-value:5.58E-10||SGD DESC:Allantoinase, converts allantoin to allantoate in the first step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DAL7(YIR031C)|FD-Score:3.13|P-value:8.69E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DOA1(YKL213C)|FD-Score:-3.36|P-value:3.90E-4||SGD DESC:WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress Gene:DYN3(YMR299C)|FD-Score:5.14|P-value:1.34E-7||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:ECM19(YLR390W)|FD-Score:5.71|P-value:5.69E-9||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:ELO1(YJL196C)|FD-Score:-4.98|P-value:3.15E-7||SGD DESC:Elongase I, medium-chain acyl elongase; catalyzes carboxy-terminal elongation of unsaturated C12-C16 fatty acyl-CoAs to C16-C18 fatty acids; ELO1 has a paralog, FEN1, that arose from the whole genome duplication Gene:EMI2(YDR516C)|FD-Score:-3.94|P-value:4.03E-5||SGD DESC:Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:EPS1(YIL005W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family Gene:ERG28(YER044C)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:Endoplasmic reticulum membrane protein, may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p Gene:EXO1(YOR033C)|FD-Score:4.02|P-value:2.91E-5||SGD DESC:5'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair and DNA mismatch repair; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress Gene:FDC1(YDR539W)|FD-Score:-5.54|P-value:1.55E-8||SGD DESC:Putative phenylacrylic acid decarboxylase; essential for the decarboxylation of aromatic carboxylic acids to the corresponding vinyl derivatives; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm Gene:FPR3(YML074C)|FD-Score:-4.74|P-value:1.09E-6||SGD DESC:Nucleolar peptidyl-prolyl cis-trans isomerase (PPIase); FK506 binding protein; phosphorylated by casein kinase II (Cka1p-Cka2p-Ckb1p-Ckb2p) and dephosphorylated by Ptp1p Gene:GEM1(YAL048C)|FD-Score:-3.19|P-value:7.11E-4||SGD DESC:Outer mitochondrial membrane GTPase, subunit of the ERMES complex; potential regulatory subunit of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; cells lacking Gem1p contain collapsed, globular, or grape-like mitochondria; ortholog of metazoan Miro GTPases Gene:GET4(YOR164C)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Mdy2p; highly conserved across species and homologous to human gene C7orf20 Gene:GPM3(YOL056W)|FD-Score:3.19|P-value:7.03E-4||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:GSY2(YLR258W)|FD-Score:9.12|P-value:3.78E-20||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:GTF1(YGR102C)|FD-Score:4.05|P-value:2.58E-5||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; transposon insertion mutant is salt sensitive and null mutant has growth defects; non-tagged protein is detected in purified mitochondria Gene:HMG1(YML075C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress Gene:HNT3(YOR258W)|FD-Score:-4.62|P-value:1.93E-6||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:HSM3(YBR272C)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation Gene:HST2(YPL015C)|FD-Score:-3.48|P-value:2.48E-4||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:HXT17(YNR072W)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Hexose transporter, up-regulated in media containing raffinose and galactose at pH 7.7 versus pH 4.7, repressed by high levels of glucose Gene:IGO1(YNL157W)|FD-Score:-3.17|P-value:7.60E-4||SGD DESC:Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo2p Gene:INP2(YMR163C)|FD-Score:-3.39|P-value:3.46E-4||SGD DESC:Peroxisome-specific receptor important for peroxisome inheritance; co-fractionates with peroxisome membranes and co-localizes with peroxisomes in vivo; physically interacts with the myosin V motor Myo2p; INP2 is not an essential gene Gene:ISY1(YJR050W)|FD-Score:4.46|P-value:4.13E-6||SGD DESC:Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles Gene:KDX1(YKL161C)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p Gene:LYS14(YDR034C)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Transcriptional activator involved in regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer Gene:MEU1(YLR017W)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Methylthioadenosine phosphorylase (MTAP), catalyzes the initial step in the methionine salvage pathway; affects polyamine biosynthesis through regulation of ornithine decarboxylase (Spe1p) activity; regulates ADH2 gene expression Gene:MND2(YIR025W)|FD-Score:4.15|P-value:1.68E-5||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MNN11(YJL183W)|FD-Score:-3.8|P-value:7.15E-5||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MRPL11(YDL202W)|FD-Score:3.77|P-value:8.12E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NIS1(YNL078W)|FD-Score:-3.16|P-value:7.85E-4||SGD DESC:Protein localized in the bud neck at G2/M phase; physically interacts with septins; possibly involved in a mitotic signaling network Gene:NVJ1(YHR195W)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) Gene:PDR8(YLR266C)|FD-Score:4.63|P-value:1.84E-6||SGD DESC:Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication Gene:PFD1(YJL179W)|FD-Score:4.12|P-value:1.88E-5||SGD DESC:Subunit of heterohexameric prefoldin, which binds cytosolic chaperonin and transfers target proteins to it; involved in the biogenesis of actin and of alpha- and gamma-tubulin Gene:PMS1(YNL082W)|FD-Score:-3.14|P-value:8.56E-4||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:PRM4(YPL156C)|FD-Score:-3.18|P-value:7.31E-4||SGD DESC:Pheromone-regulated protein proposed to be involved in mating; predicted to have 1 transmembrane segment; transcriptionally regulated by Ste12p during mating and by Cat8p during the diauxic shift Gene:RAD2(YGR258C)|FD-Score:3.16|P-value:7.77E-4||SGD DESC:Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein Gene:RAD59(YDL059C)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p Gene:RIM13(YMR154C)|FD-Score:8.64|P-value:2.86E-18||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RLM1(YPL089C)|FD-Score:-4.73|P-value:1.13E-6||SGD DESC:MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication Gene:ROX1(YPR065W)|FD-Score:-3.22|P-value:6.41E-4||SGD DESC:Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance Gene:SAC3(YDR159W)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SIP3(YNL257C)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication Gene:SLX8(YER116C)|FD-Score:-4.11|P-value:1.95E-5||SGD DESC:Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress Gene:SNF5(YBR289W)|FD-Score:-4.3|P-value:8.70E-6||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions Gene:SRC1(YML034W)|FD-Score:5.74|P-value:4.80E-9||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:STI1(YOR027W)|FD-Score:-4.85|P-value:6.19E-7||SGD DESC:Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop Gene:TCB3(YML072C)|FD-Score:4.17|P-value:1.49E-5||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:TDA7(YNL176C_p)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Cell cycle-regulated gene of unknown function, promoter bound by Fkh2p; null mutant is sensitive to expression of the top1-T722A allele Gene:TRF5(YNL299W)|FD-Score:-4.78|P-value:8.65E-7||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p Gene:TRP3(YKL211C)|FD-Score:6.46|P-value:5.11E-11||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TVP18(YMR071C)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:UFD4(YKL010C)|FD-Score:-4.71|P-value:1.24E-6||SGD DESC:Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress Gene:URA2(YJL130C)|FD-Score:4.75|P-value:1.02E-6||SGD DESC:Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP Gene:UTR1(YJR049C)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VPS45(YGL095C)|FD-Score:-5.53|P-value:1.56E-8||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS63(YLR261C_d)|FD-Score:-3.26|P-value:5.49E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:XDJ1(YLR090W)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Chaperone with a role in facilitating mitochondrial protein import; ascomycete-specific member of the DnaJ-like family, closely related to Ydj1p; predicted to be C-terminally prenylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YBL012C(YBL012C_d)|FD-Score:4.94|P-value:3.94E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCL075W(YCL075W)|FD-Score:4.01|P-value:3.06E-5||SGD DESC:Pseudogene: encodes fragment of Ty Pol protein Gene:YDJ1(YNL064C)|FD-Score:-4.54|P-value:2.88E-6||SGD DESC:Type I HSP40 co-chaperone; involved in regulation of HSP90 and HSP70 functions; critical for determining cell size at Start as a function of growth rate; involved in protein translocation across membranes; member of the DnaJ family Gene:YDR444W(YDR444W_p)|FD-Score:-4.15|P-value:1.63E-5||SGD DESC:Putative hydrolase acting on ester bonds Gene:YEL067C(YEL067C_p)|FD-Score:-3.44|P-value:2.90E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YET3(YDL072C)|FD-Score:7.68|P-value:8.06E-15||SGD DESC:Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress Gene:YGL041C-B(YGL041C-B_p)|FD-Score:-4.5|P-value:3.41E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YHL008C(YHL008C_p)|FD-Score:6.02|P-value:8.52E-10||SGD DESC:Putative protein of unknown function, may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YHL015W-A(YHL015W-A_p)|FD-Score:4.37|P-value:6.29E-6||SGD DESC:Putative protein of unknown function Gene:YHR140W(YHR140W_p)|FD-Score:3.95|P-value:3.93E-5||SGD DESC:Putative integral membrane protein of unknown function Gene:YIL102C(YIL102C_p)|FD-Score:-3.75|P-value:8.81E-5||SGD DESC:Putative protein of unknown function Gene:YIL161W(YIL161W_p)|FD-Score:-4.2|P-value:1.31E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; mRNA is enriched in Scp160p-associated mRNPs; YIL161W is a non-essential gene Gene:YKL077W(YKL077W_p)|FD-Score:4.84|P-value:6.49E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YKL097C(YKL097C_d)|FD-Score:-3.43|P-value:3.04E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species Gene:YKR070W(YKR070W_p)|FD-Score:3.9|P-value:4.82E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLR202C(YLR202C_d)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron Gene:YLR279W(YLR279W_d)|FD-Score:4.23|P-value:1.17E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR349W(YLR349W_d)|FD-Score:-3.21|P-value:6.54E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified ORF DIC1/YLR348C Gene:YLR419W(YLR419W_p)|FD-Score:-4.59|P-value:2.18E-6||SGD DESC:Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene Gene:YML057C-A(YML057C-A_d)|FD-Score:-4.59|P-value:2.22E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene CMP2/YML057W Gene:YMR182W-A(YMR182W-A_p)|FD-Score:-4.43|P-value:4.61E-6||SGD DESC:Putative protein of unknown function Gene:YMR279C(YMR279C)|FD-Score:4.85|P-value:6.29E-7||SGD DESC:Putative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; YMR279C has a paralog, ATR1, that arose from the whole genome duplication Gene:YNL050C(YNL050C_p)|FD-Score:4.02|P-value:2.92E-5||SGD DESC:Putative protein of unknown function; YNL050c is not an essential gene Gene:YOR314W(YOR314W_d)|FD-Score:4.07|P-value:2.34E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL062W(YPL062W_d)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation Gene:YPL162C(YPL162C_p)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology Gene:YPL199C(YPL199C_p)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Putative protein of unknown function, predicted to be palmitoylated Gene:YPQ1(YOL092W_p)|FD-Score:3.16|P-value:7.93E-4||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:YPR014C(YPR014C_d)|FD-Score:5.72|P-value:5.45E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene Gene:YPR077C(YPR077C_d)|FD-Score:3.84|P-value:6.19E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR072W9.979.84E-241.92ERG7Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis
YHR007C8.392.34E-171.92ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YLR033W6.873.16E-121.92RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YLR321C4.953.72E-70.04SFH1Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog
YPL209C4.914.60E-70.03IPL1Aurora kinase subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; required for SPB cohesion and prevention of multipolar spindle formation; localizes to nuclear foci that become diffuse upon DNA replication stress; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon SAC arrest
YOR157C4.885.40E-70.21PUP1Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z
YIL115C4.671.53E-60.21NUP159FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p)
YOR319W4.454.20E-60.24HSH49U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
YML127W4.211.25E-50.52RSC9Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
YER146W3.701.08E-40.02LSM5Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA
YJL050W3.681.15E-40.01MTR4ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing
YFR037C3.681.18E-40.17RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YDR016C3.512.25E-40.12DAD1Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
YPR113W3.383.56E-40.21PIS1Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins
YLR298C3.177.52E-40.03YHC1Component of the U1 snRNP complex required for pre-mRNA splicing; putative ortholog of human U1C protein, which is involved in formation of a complex between U1 snRNP and the pre-mRNA 5' splice site

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR258W9.123.78E-20GSY2Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress
YMR154C8.642.86E-18RIM13Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB
YDL072C7.688.06E-15YET3Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress
YBR131W6.719.63E-12CCZ1Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway
YKL211C6.465.11E-11TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YIR027C6.095.58E-10DAL1Allantoinase, converts allantoin to allantoate in the first step of allantoin degradation; expression sensitive to nitrogen catabolite repression
YHL008C_p6.028.52E-10YHL008C_pPutative protein of unknown function, may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
YML034W5.744.80E-9SRC1Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance
YPR014C_d5.725.45E-9YPR014C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene
YLR390W5.715.69E-9ECM19Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YMR299C5.141.34E-7DYN3Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration
YBL012C_d4.943.94E-7YBL012C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YMR279C4.856.29E-7YMR279CPutative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; YMR279C has a paralog, ATR1, that arose from the whole genome duplication
YKL077W_p4.846.49E-7YKL077W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
YJL130C4.751.02E-6URA2Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP

GO enrichment analysis for SGTC_2971
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1782.45E-43SGTC_29749094440 9.7 μMChembridge (Drug-like library)252361340.795918ergosterol biosynthesis
0.1603.71E-35SGTC_643k048-0106 28.4 μMChemDiv (Drug-like library)67639200.0921053RSC & ERG11
0.1547.80E-33SGTC_13171273-0028 910.0 nMChemDiv (Drug-like library)59178030.057971azole & statin
0.1512.13E-31SGTC_2648atorvastatin 78.7 μMMiscellaneous608230.0957447azole & statin
0.1426.69E-28SGTC_3241315-0088 19.6 μMChemDiv (Drug-like library)67874210.0886076RSC & ERG11
0.1382.04E-26SGTC_5554296-0685 37.0 μMChemDiv (Drug-like library)29085400.097561ergosterol biosynthesis
0.1369.95E-26SGTC_917harmaline 728.1 nMTimTec (Natural product library)52809510.112676ergosterol biosynthesis
0.1323.19E-24SGTC_23779073351 62.6 μMChembridge (Fragment library)73379460.253968ergosterol biosynthesis
0.1271.05E-22SGTC_7830848-0141 92.7 μMChemDiv (Drug-like library)67550760.0769231RSC & ERG11
0.1219.14E-21SGTC_21856113692 200.0 μMChembridge (Fragment library)8741500.153846ergosterol biosynthesis
0.1172.22E-19SGTC_9701315-0089 19.5 μMChemDiv (Drug-like library)68110830.120482RSC & ERG11
0.1155.95E-19SGTC_3271273-0057 4.0 μMChemDiv (Drug-like library)31081380.0714286azole & statin
0.1141.64E-18SGTC_1931273-0060 1.6 μMChemDiv (Drug-like library)28473930.056338ergosterol biosynthesis
0.1132.57E-18SGTC_12370330-0133 46.1 μMChemDiv (Drug-like library)X12370.0857143ergosterol biosynthesis
0.1111.56E-17SGTC_329k048-0121 21.3 μMChemDiv (Drug-like library)68329020.0547945RSC & ERG11

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3152909925349.47 μM0.812525236131Chembridge (Drug-like library)322.852863.45213NEO1-PIK1
SGTC_297490944409.66 μM0.79591825236134Chembridge (Drug-like library)387.722343.71413ergosterol biosynthesis
SGTC_2973909466517.96 μM0.76470625237061Chembridge (Drug-like library)336.879443.90813NEO1
SGTC_3037909102649.47 μM0.625236398Chembridge (Drug-like library)387.722343.71413NEO1-PIK1
SGTC_3057909594649.47 μM0.44067824540043Chembridge (Drug-like library)311.8021034.43603Golgi
SGTC_3154910011349.47 μM0.43548425237525Chembridge (Drug-like library)336.879444.39713
SGTC_2910794840525.97 μM0.388062971328Chembridge (Drug-like library)417.748323.49214plasma membrane duress
SGTC_3155909926549.47 μM0.38095225236499Chembridge (Drug-like library)330.83184.15213
SGTC_3141909460949.47 μM0.36507925236309Chembridge (Drug-like library)381.67474.03713iron homeostasis
SGTC_3024909063149.47 μM0.30769225236383Chembridge (Drug-like library)311.8021034.43603