9009005

4-ethyl-N-(pyridin-3-ylmethylcarbamothioyl)benzamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2976
Screen concentration 19.5 μM
Source Chembridge (Drug-like library)
PubChem CID 2993148
SMILES CCC1=CC=C(C=C1)C(=O)NC(=S)NCC2=CN=CC=C2
Standardized SMILES CCc1ccc(cc1)C(=O)NC(=S)NCc2cccnc2
Molecular weight 299.3907
ALogP 3.29
H-bond donor count 2
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.56
% growth inhibition (Hom. pool) 7.33


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2993148
Download HIP data (tab-delimited text)  (excel)
Gene:CCT7(YJL111W)|FD-Score:4.27|P-value:9.92E-6|Clearance:0.65||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC12(YHR107C)|FD-Score:4.74|P-value:1.08E-6|Clearance:0.65||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC15(YAR019C)|FD-Score:-4.38|P-value:6.04E-6|Clearance:0||SGD DESC:Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress Gene:CDC25(YLR310C)|FD-Score:4.59|P-value:2.23E-6|Clearance:0.65||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:DAM1(YGR113W)|FD-Score:5.12|P-value:1.54E-7|Clearance:0.65||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments Gene:FCF2(YLR051C)|FD-Score:-3.3|P-value:4.85E-4|Clearance:0||SGD DESC:Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon Gene:KAR1(YNL188W)|FD-Score:-3.41|P-value:3.26E-4|Clearance:0||SGD DESC:Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p Gene:NBP35(YGL091C)|FD-Score:-4.37|P-value:6.30E-6|Clearance:0||SGD DESC:Essential iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Cfd1p that is involved in iron-sulfur protein assembly in the cytosol; similar to P-loop NTPases Gene:POP6(YGR030C)|FD-Score:4.51|P-value:3.20E-6|Clearance:0.65||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RFA1(YAR007C)|FD-Score:-3.54|P-value:1.97E-4|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination Gene:RHO3(YIL118W)|FD-Score:-3.71|P-value:1.02E-4|Clearance:0||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p Gene:RIA1(YNL163C)|FD-Score:3.33|P-value:4.40E-4|Clearance:0.1||SGD DESC:Cytoplasmic GTPase/eEF2-like factor involved in ribosomal biogenesis; with Sdo1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; required for quality control check of newly made large ribosomal subunits before they are released into the pool of translating ribosomes Gene:RPB2(YOR151C)|FD-Score:-3.43|P-value:3.01E-4|Clearance:0||SGD DESC:RNA polymerase II second largest subunit B150, part of central core; similar to bacterial beta subunit Gene:RPL18A(YOL120C)|FD-Score:3.11|P-value:9.29E-4|Clearance:0.04||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPP1(YHR062C)|FD-Score:4.78|P-value:8.72E-7|Clearance:0.65||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RSC58(YLR033W)|FD-Score:-3.35|P-value:4.10E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC8(YFR037C)|FD-Score:-4.42|P-value:5.00E-6|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SMC4(YLR086W)|FD-Score:-3.67|P-value:1.19E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate Gene:SPT15(YER148W)|FD-Score:3.22|P-value:6.38E-4|Clearance:0.11||SGD DESC:TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability Gene:TSR1(YDL060W)|FD-Score:3.54|P-value:1.98E-4|Clearance:0.22||SGD DESC:Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:YEF3(YLR249W)|FD-Score:4.75|P-value:1.04E-6|Clearance:0.65||SGD DESC:Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication Gene:YKL083W(YKL083W_d)|FD-Score:4.3|P-value:8.39E-6|Clearance:0.65||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene RRP14 Gene:YPP1(YGR198W)|FD-Score:3.62|P-value:1.48E-4|Clearance:0.08||SGD DESC:Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene Gene:CCT7(YJL111W)|FD-Score:4.27|P-value:9.92E-6|Clearance:0.65||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC12(YHR107C)|FD-Score:4.74|P-value:1.08E-6|Clearance:0.65||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC15(YAR019C)|FD-Score:-4.38|P-value:6.04E-6|Clearance:0||SGD DESC:Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress Gene:CDC25(YLR310C)|FD-Score:4.59|P-value:2.23E-6|Clearance:0.65||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:DAM1(YGR113W)|FD-Score:5.12|P-value:1.54E-7|Clearance:0.65||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments Gene:FCF2(YLR051C)|FD-Score:-3.3|P-value:4.85E-4|Clearance:0||SGD DESC:Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon Gene:KAR1(YNL188W)|FD-Score:-3.41|P-value:3.26E-4|Clearance:0||SGD DESC:Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p Gene:NBP35(YGL091C)|FD-Score:-4.37|P-value:6.30E-6|Clearance:0||SGD DESC:Essential iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Cfd1p that is involved in iron-sulfur protein assembly in the cytosol; similar to P-loop NTPases Gene:POP6(YGR030C)|FD-Score:4.51|P-value:3.20E-6|Clearance:0.65||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RFA1(YAR007C)|FD-Score:-3.54|P-value:1.97E-4|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination Gene:RHO3(YIL118W)|FD-Score:-3.71|P-value:1.02E-4|Clearance:0||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p Gene:RIA1(YNL163C)|FD-Score:3.33|P-value:4.40E-4|Clearance:0.1||SGD DESC:Cytoplasmic GTPase/eEF2-like factor involved in ribosomal biogenesis; with Sdo1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; required for quality control check of newly made large ribosomal subunits before they are released into the pool of translating ribosomes Gene:RPB2(YOR151C)|FD-Score:-3.43|P-value:3.01E-4|Clearance:0||SGD DESC:RNA polymerase II second largest subunit B150, part of central core; similar to bacterial beta subunit Gene:RPL18A(YOL120C)|FD-Score:3.11|P-value:9.29E-4|Clearance:0.04||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPP1(YHR062C)|FD-Score:4.78|P-value:8.72E-7|Clearance:0.65||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RSC58(YLR033W)|FD-Score:-3.35|P-value:4.10E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC8(YFR037C)|FD-Score:-4.42|P-value:5.00E-6|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SMC4(YLR086W)|FD-Score:-3.67|P-value:1.19E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate Gene:SPT15(YER148W)|FD-Score:3.22|P-value:6.38E-4|Clearance:0.11||SGD DESC:TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability Gene:TSR1(YDL060W)|FD-Score:3.54|P-value:1.98E-4|Clearance:0.22||SGD DESC:Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:YEF3(YLR249W)|FD-Score:4.75|P-value:1.04E-6|Clearance:0.65||SGD DESC:Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication Gene:YKL083W(YKL083W_d)|FD-Score:4.3|P-value:8.39E-6|Clearance:0.65||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene RRP14 Gene:YPP1(YGR198W)|FD-Score:3.62|P-value:1.48E-4|Clearance:0.08||SGD DESC:Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2993148
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:4.05|P-value:2.55E-5||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:AEP3(YPL005W)|FD-Score:-3.35|P-value:4.06E-4||SGD DESC:Protein that may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; localized to the matrix face of the mitochondrial inner membrane; stabilizes the bicistronic AAP1-ATP6 mRNA Gene:AIM4(YBR194W)|FD-Score:-3.11|P-value:9.45E-4||SGD DESC:Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress Gene:AKL1(YBR059C)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Ser-Thr protein kinase, member (with Ark1p and Prk1p) of the Ark kinase family; involved in endocytosis and actin cytoskeleton organization Gene:APL5(YPL195W)|FD-Score:-3.12|P-value:9.17E-4||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:ARO1(YDR127W)|FD-Score:25|P-value:1.96E-138||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:9.13|P-value:3.37E-20||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ARO7(YPR060C)|FD-Score:12.7|P-value:4.61E-37||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ARP6(YLR085C)|FD-Score:-3.16|P-value:7.83E-4||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:AYR1(YIL124W)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase found in lipid particles, ER, and mitochondrial outer membrane; involved in phosphatidic acid biosynthesis; required for spore germination; capable of metabolizing steroid hormones Gene:BAP2(YBR068C)|FD-Score:19.1|P-value:5.05E-82||SGD DESC:High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication Gene:BNI4(YNL233W)|FD-Score:-4.93|P-value:4.18E-7||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:BSC4(YNL269W)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p Gene:CCE1(YKL011C)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA Gene:CCW12(YLR110C)|FD-Score:4.48|P-value:3.65E-6||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:COG6(YNL041C)|FD-Score:-6.08|P-value:5.89E-10||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CUL3(YGR003W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Ubiquitin-protein ligase, forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3 Gene:CWP2(YKL096W-A)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Covalently linked cell wall mannoprotein, major constituent of the cell wall; plays a role in stabilizing the cell wall; involved in low pH resistance; precursor is GPI-anchored Gene:DAL81(YIR023W)|FD-Score:14.8|P-value:9.55E-50||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DMA1(YHR115C)|FD-Score:-3.8|P-value:7.11E-5||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger Gene:DSS1(YMR287C)|FD-Score:-3.44|P-value:2.90E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:ELP4(YPL101W)|FD-Score:-3.32|P-value:4.54E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:GLY1(YEL046C)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GOR1(YNL274C)|FD-Score:6.99|P-value:1.38E-12||SGD DESC:Glyoxylate reductase; null mutation results in increased biomass after diauxic shift; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:GYP1(YOR070C)|FD-Score:5.5|P-value:1.87E-8||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HNM1(YGL077C)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:Choline/ethanolamine transporter; involved in the uptake of nitrogen mustard and the uptake of glycine betaine during hypersaline stress; co-regulated with phospholipid biosynthetic genes and negatively regulated by choline and myo-inositol Gene:IOC3(YFR013W)|FD-Score:4.01|P-value:2.99E-5||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:JLP2(YMR132C_p)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Protein of unknown function, contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein) Gene:KEL1(YHR158C)|FD-Score:-3.25|P-value:5.69E-4||SGD DESC:Protein required for proper cell fusion and cell morphology; functions in a complex with Kel2p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:LEU3(YLR451W)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Zinc-knuckle transcription factor, repressor and activator; regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; acts as a repressor in leucine-replete conditions and as an activator in the presence of alpha-isopropylmalate, an intermediate in leucine biosynthesis that accumulates during leucine starvation Gene:LHS1(YKL073W)|FD-Score:4.97|P-value:3.42E-7||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:MAL32(YBR299W)|FD-Score:3.36|P-value:3.87E-4||SGD DESC:Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose Gene:MDM32(YOR147W)|FD-Score:3.98|P-value:3.48E-5||SGD DESC:Mitochondrial inner membrane protein with similarity to Mdm31p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34 Gene:MIC17(YMR002W)|FD-Score:-5.99|P-value:1.06E-9||SGD DESC:Mitochondrial intermembrane space protein; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MOT3(YMR070W)|FD-Score:3.91|P-value:4.60E-5||SGD DESC:Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion Gene:MRK1(YDL079C)|FD-Score:5.1|P-value:1.73E-7||SGD DESC:Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation Gene:NRG2(YBR066C)|FD-Score:3.27|P-value:5.32E-4||SGD DESC:Transcriptional repressor; mediates glucose repression and negatively regulates filamentous growth; NRG2 has a paralog, NRG1, that arose from the whole genome duplication Gene:OST5(YGL226C-A)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Zeta subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:PCL5(YHR071W)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Cyclin, interacts with and phosphorylated by Pho85p cyclin-dependent kinase (Cdk), induced by Gcn4p at level of transcription, specifically required for Gcn4p degradation, may be sensor of cellular protein biosynthetic capacity Gene:PEP3(YLR148W)|FD-Score:-3.32|P-value:4.57E-4||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PEP7(YDR323C)|FD-Score:-3.57|P-value:1.76E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PEX14(YGL153W)|FD-Score:4.82|P-value:7.28E-7||SGD DESC:Peroxisomal membrane peroxin that is a central component of the peroxisomal protein import machinery; interacts with both PTS1 (Pex5p) and PTS2 (Pex7p), peroxisomal matrix protein signal recognition factors and membrane receptor Pex13p Gene:PPT2(YPL148C)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PRS3(YHL011C)|FD-Score:5.74|P-value:4.62E-9||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:RAD52(YML032C)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Protein that stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis Gene:RBG2(YGR173W)|FD-Score:-4.42|P-value:5.04E-6||SGD DESC:Protein with a role in translation; forms a complex with Gir2p; has similarity to mammalian developmentally regulated GTP-binding protein Gene:RGP1(YDR137W)|FD-Score:-3.35|P-value:3.97E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RPL20B(YOR312C)|FD-Score:-3.22|P-value:6.31E-4||SGD DESC:Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication Gene:RPL34B(YIL052C)|FD-Score:5.19|P-value:1.05E-7||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:-4.14|P-value:1.76E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS7A(YOR096W)|FD-Score:-3.95|P-value:3.88E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7A has a paralog, RPS7B, that arose from the whole genome duplication Gene:RRI2(YOL117W)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:RTG1(YOL067C)|FD-Score:-7.24|P-value:2.21E-13||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:SCD6(YPR129W)|FD-Score:-3.09|P-value:9.95E-4||SGD DESC:Repressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1; forms cytoplasmic foci upon DNA replication stress Gene:SGE1(YPR198W)|FD-Score:3.1|P-value:9.51E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SGT2(YOR007C)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; has similarity to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress Gene:SNF2(YOR290C)|FD-Score:4.65|P-value:1.64E-6||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SNX4(YJL036W)|FD-Score:4.43|P-value:4.64E-6||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SPO7(YAL009W)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:SPT3(YDR392W)|FD-Score:17.3|P-value:1.74E-67||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SPT4(YGR063C)|FD-Score:9.67|P-value:1.99E-22||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:STR2(YJR130C)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Cystathionine gamma-synthase, converts cysteine into cystathionine Gene:THP2(YHR167W)|FD-Score:3.73|P-value:9.60E-5||SGD DESC:Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance Gene:TPN1(YGL186C)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TPP1(YMR156C)|FD-Score:-3.27|P-value:5.44E-4||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:UFD2(YDL190C)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3 Gene:UPS2(YLR168C)|FD-Score:5.19|P-value:1.06E-7||SGD DESC:Mitochondrial protein involved in phospholipid metabolism; localizes to the intermembrane space; has a role in regulation of phospholipid metabolism by inhibiting conversion of phosphatidylethanolamine to phosphatidylcholine; null mutant has defects in mitochondrial morphology; similar to Ups1p, Ups3p and to human PRELI Gene:VPS35(YJL154C)|FD-Score:3.39|P-value:3.56E-4||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:VPS45(YGL095C)|FD-Score:-4.27|P-value:9.82E-6||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YBR238C(YBR238C)|FD-Score:-3.38|P-value:3.68E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YCK3(YER123W)|FD-Score:-3.27|P-value:5.41E-4||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YCL021W-A(YCL021W-A_p)|FD-Score:3.11|P-value:9.51E-4||SGD DESC:Putative protein of unknown function Gene:YDL027C(YDL027C_p)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL027C is not an essential gene Gene:YDR340W(YDR340W_d)|FD-Score:7.92|P-value:1.23E-15||SGD DESC:Putative protein of unknown function Gene:YFL013W-A(YFL013W-A_d)|FD-Score:-3.5|P-value:2.31E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps IES1/YFL013C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching Gene:YFL054C(YFL054C_p)|FD-Score:4.06|P-value:2.50E-5||SGD DESC:Putative channel-like protein; similar to Fps1p; mediates passive diffusion of glycerol in the presence of ethanol Gene:YGR064W(YGR064W_d)|FD-Score:4.05|P-value:2.57E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YHR095W(YHR095W_d)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL163C(YIL163C_p)|FD-Score:-6.51|P-value:3.80E-11||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YJR020W(YJR020W_d)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR115W(YJR115W_p)|FD-Score:6.23|P-value:2.39E-10||SGD DESC:Putative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication Gene:YMR084W(YMR084W_p)|FD-Score:5.65|P-value:7.92E-9||SGD DESC:Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YMR166C(YMR166C_p)|FD-Score:6|P-value:1.02E-9||SGD DESC:Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene Gene:YMR226C(YMR226C)|FD-Score:3.31|P-value:4.67E-4||SGD DESC:NADP(+)-dependent dehydrogenase; acts on serine, L-allo-threonine, and other 3-hydroxy acids; green fluorescent protein fusion protein localizes to the cytoplasm and nucleus; may interact with ribosomes, based on co-purification experiments Gene:YMR245W(YMR245W_d)|FD-Score:5.94|P-value:1.42E-9||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR252C(YMR252C_p)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YMR252C is not an essential gene Gene:YND1(YER005W)|FD-Score:-3.1|P-value:9.53E-4||SGD DESC:Apyrase with wide substrate specificity, helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity Gene:YNL089C(YNL089C_d)|FD-Score:3.15|P-value:8.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; almost completely overlaps YNL090W/RHO2 which encodes a small GTPase of the Rho/Rac subfamily of Ras-like proteins Gene:YNR005C(YNR005C_d)|FD-Score:-3.31|P-value:4.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR042W(YNR042W_d)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2 Gene:YOL038C-A(YOL038C-A_p)|FD-Score:-3.32|P-value:4.56E-4||SGD DESC:Putative protein of unknown function; identified by SAGE analysis Gene:YOR186W(YOR186W_p)|FD-Score:-3.17|P-value:7.66E-4||SGD DESC:Putative protein of unknown function; proper regulation of expression during heat stress is sphingolipid-dependent; YOR186W has a paralog, YLR297W, that arose from the whole genome duplication Gene:YPT31(YER031C)|FD-Score:-4.3|P-value:8.50E-6||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:YUR1(YJL139C)|FD-Score:-3.39|P-value:3.51E-4||SGD DESC:Mannosyltransferase involved in protein N-glycosylation; member of the KTR1 family; located in the Golgi apparatus; YUR1 has a paralog, KTR2, that arose from the whole genome duplication Gene:ACB1(YGR037C)|FD-Score:4.05|P-value:2.55E-5||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:AEP3(YPL005W)|FD-Score:-3.35|P-value:4.06E-4||SGD DESC:Protein that may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; localized to the matrix face of the mitochondrial inner membrane; stabilizes the bicistronic AAP1-ATP6 mRNA Gene:AIM4(YBR194W)|FD-Score:-3.11|P-value:9.45E-4||SGD DESC:Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress Gene:AKL1(YBR059C)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Ser-Thr protein kinase, member (with Ark1p and Prk1p) of the Ark kinase family; involved in endocytosis and actin cytoskeleton organization Gene:APL5(YPL195W)|FD-Score:-3.12|P-value:9.17E-4||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:ARO1(YDR127W)|FD-Score:25|P-value:1.96E-138||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:9.13|P-value:3.37E-20||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ARO7(YPR060C)|FD-Score:12.7|P-value:4.61E-37||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ARP6(YLR085C)|FD-Score:-3.16|P-value:7.83E-4||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:AYR1(YIL124W)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase found in lipid particles, ER, and mitochondrial outer membrane; involved in phosphatidic acid biosynthesis; required for spore germination; capable of metabolizing steroid hormones Gene:BAP2(YBR068C)|FD-Score:19.1|P-value:5.05E-82||SGD DESC:High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication Gene:BNI4(YNL233W)|FD-Score:-4.93|P-value:4.18E-7||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:BSC4(YNL269W)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p Gene:CCE1(YKL011C)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA Gene:CCW12(YLR110C)|FD-Score:4.48|P-value:3.65E-6||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:COG6(YNL041C)|FD-Score:-6.08|P-value:5.89E-10||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CUL3(YGR003W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Ubiquitin-protein ligase, forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3 Gene:CWP2(YKL096W-A)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Covalently linked cell wall mannoprotein, major constituent of the cell wall; plays a role in stabilizing the cell wall; involved in low pH resistance; precursor is GPI-anchored Gene:DAL81(YIR023W)|FD-Score:14.8|P-value:9.55E-50||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DMA1(YHR115C)|FD-Score:-3.8|P-value:7.11E-5||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger Gene:DSS1(YMR287C)|FD-Score:-3.44|P-value:2.90E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:ELP4(YPL101W)|FD-Score:-3.32|P-value:4.54E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:GLY1(YEL046C)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GOR1(YNL274C)|FD-Score:6.99|P-value:1.38E-12||SGD DESC:Glyoxylate reductase; null mutation results in increased biomass after diauxic shift; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:GYP1(YOR070C)|FD-Score:5.5|P-value:1.87E-8||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HNM1(YGL077C)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:Choline/ethanolamine transporter; involved in the uptake of nitrogen mustard and the uptake of glycine betaine during hypersaline stress; co-regulated with phospholipid biosynthetic genes and negatively regulated by choline and myo-inositol Gene:IOC3(YFR013W)|FD-Score:4.01|P-value:2.99E-5||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:JLP2(YMR132C_p)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Protein of unknown function, contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein) Gene:KEL1(YHR158C)|FD-Score:-3.25|P-value:5.69E-4||SGD DESC:Protein required for proper cell fusion and cell morphology; functions in a complex with Kel2p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:LEU3(YLR451W)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Zinc-knuckle transcription factor, repressor and activator; regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; acts as a repressor in leucine-replete conditions and as an activator in the presence of alpha-isopropylmalate, an intermediate in leucine biosynthesis that accumulates during leucine starvation Gene:LHS1(YKL073W)|FD-Score:4.97|P-value:3.42E-7||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:MAL32(YBR299W)|FD-Score:3.36|P-value:3.87E-4||SGD DESC:Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose Gene:MDM32(YOR147W)|FD-Score:3.98|P-value:3.48E-5||SGD DESC:Mitochondrial inner membrane protein with similarity to Mdm31p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34 Gene:MIC17(YMR002W)|FD-Score:-5.99|P-value:1.06E-9||SGD DESC:Mitochondrial intermembrane space protein; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MOT3(YMR070W)|FD-Score:3.91|P-value:4.60E-5||SGD DESC:Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion Gene:MRK1(YDL079C)|FD-Score:5.1|P-value:1.73E-7||SGD DESC:Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation Gene:NRG2(YBR066C)|FD-Score:3.27|P-value:5.32E-4||SGD DESC:Transcriptional repressor; mediates glucose repression and negatively regulates filamentous growth; NRG2 has a paralog, NRG1, that arose from the whole genome duplication Gene:OST5(YGL226C-A)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Zeta subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:PCL5(YHR071W)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Cyclin, interacts with and phosphorylated by Pho85p cyclin-dependent kinase (Cdk), induced by Gcn4p at level of transcription, specifically required for Gcn4p degradation, may be sensor of cellular protein biosynthetic capacity Gene:PEP3(YLR148W)|FD-Score:-3.32|P-value:4.57E-4||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PEP7(YDR323C)|FD-Score:-3.57|P-value:1.76E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PEX14(YGL153W)|FD-Score:4.82|P-value:7.28E-7||SGD DESC:Peroxisomal membrane peroxin that is a central component of the peroxisomal protein import machinery; interacts with both PTS1 (Pex5p) and PTS2 (Pex7p), peroxisomal matrix protein signal recognition factors and membrane receptor Pex13p Gene:PPT2(YPL148C)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PRS3(YHL011C)|FD-Score:5.74|P-value:4.62E-9||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:RAD52(YML032C)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Protein that stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis Gene:RBG2(YGR173W)|FD-Score:-4.42|P-value:5.04E-6||SGD DESC:Protein with a role in translation; forms a complex with Gir2p; has similarity to mammalian developmentally regulated GTP-binding protein Gene:RGP1(YDR137W)|FD-Score:-3.35|P-value:3.97E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RPL20B(YOR312C)|FD-Score:-3.22|P-value:6.31E-4||SGD DESC:Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication Gene:RPL34B(YIL052C)|FD-Score:5.19|P-value:1.05E-7||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:-4.14|P-value:1.76E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS7A(YOR096W)|FD-Score:-3.95|P-value:3.88E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7A has a paralog, RPS7B, that arose from the whole genome duplication Gene:RRI2(YOL117W)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:RTG1(YOL067C)|FD-Score:-7.24|P-value:2.21E-13||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:SCD6(YPR129W)|FD-Score:-3.09|P-value:9.95E-4||SGD DESC:Repressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1; forms cytoplasmic foci upon DNA replication stress Gene:SGE1(YPR198W)|FD-Score:3.1|P-value:9.51E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SGT2(YOR007C)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; has similarity to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress Gene:SNF2(YOR290C)|FD-Score:4.65|P-value:1.64E-6||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SNX4(YJL036W)|FD-Score:4.43|P-value:4.64E-6||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SPO7(YAL009W)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:SPT3(YDR392W)|FD-Score:17.3|P-value:1.74E-67||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SPT4(YGR063C)|FD-Score:9.67|P-value:1.99E-22||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:STR2(YJR130C)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Cystathionine gamma-synthase, converts cysteine into cystathionine Gene:THP2(YHR167W)|FD-Score:3.73|P-value:9.60E-5||SGD DESC:Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance Gene:TPN1(YGL186C)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TPP1(YMR156C)|FD-Score:-3.27|P-value:5.44E-4||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:UFD2(YDL190C)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3 Gene:UPS2(YLR168C)|FD-Score:5.19|P-value:1.06E-7||SGD DESC:Mitochondrial protein involved in phospholipid metabolism; localizes to the intermembrane space; has a role in regulation of phospholipid metabolism by inhibiting conversion of phosphatidylethanolamine to phosphatidylcholine; null mutant has defects in mitochondrial morphology; similar to Ups1p, Ups3p and to human PRELI Gene:VPS35(YJL154C)|FD-Score:3.39|P-value:3.56E-4||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:VPS45(YGL095C)|FD-Score:-4.27|P-value:9.82E-6||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YBR238C(YBR238C)|FD-Score:-3.38|P-value:3.68E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YCK3(YER123W)|FD-Score:-3.27|P-value:5.41E-4||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YCL021W-A(YCL021W-A_p)|FD-Score:3.11|P-value:9.51E-4||SGD DESC:Putative protein of unknown function Gene:YDL027C(YDL027C_p)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL027C is not an essential gene Gene:YDR340W(YDR340W_d)|FD-Score:7.92|P-value:1.23E-15||SGD DESC:Putative protein of unknown function Gene:YFL013W-A(YFL013W-A_d)|FD-Score:-3.5|P-value:2.31E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps IES1/YFL013C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching Gene:YFL054C(YFL054C_p)|FD-Score:4.06|P-value:2.50E-5||SGD DESC:Putative channel-like protein; similar to Fps1p; mediates passive diffusion of glycerol in the presence of ethanol Gene:YGR064W(YGR064W_d)|FD-Score:4.05|P-value:2.57E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YHR095W(YHR095W_d)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL163C(YIL163C_p)|FD-Score:-6.51|P-value:3.80E-11||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YJR020W(YJR020W_d)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR115W(YJR115W_p)|FD-Score:6.23|P-value:2.39E-10||SGD DESC:Putative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication Gene:YMR084W(YMR084W_p)|FD-Score:5.65|P-value:7.92E-9||SGD DESC:Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YMR166C(YMR166C_p)|FD-Score:6|P-value:1.02E-9||SGD DESC:Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene Gene:YMR226C(YMR226C)|FD-Score:3.31|P-value:4.67E-4||SGD DESC:NADP(+)-dependent dehydrogenase; acts on serine, L-allo-threonine, and other 3-hydroxy acids; green fluorescent protein fusion protein localizes to the cytoplasm and nucleus; may interact with ribosomes, based on co-purification experiments Gene:YMR245W(YMR245W_d)|FD-Score:5.94|P-value:1.42E-9||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR252C(YMR252C_p)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YMR252C is not an essential gene Gene:YND1(YER005W)|FD-Score:-3.1|P-value:9.53E-4||SGD DESC:Apyrase with wide substrate specificity, helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity Gene:YNL089C(YNL089C_d)|FD-Score:3.15|P-value:8.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; almost completely overlaps YNL090W/RHO2 which encodes a small GTPase of the Rho/Rac subfamily of Ras-like proteins Gene:YNR005C(YNR005C_d)|FD-Score:-3.31|P-value:4.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR042W(YNR042W_d)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2 Gene:YOL038C-A(YOL038C-A_p)|FD-Score:-3.32|P-value:4.56E-4||SGD DESC:Putative protein of unknown function; identified by SAGE analysis Gene:YOR186W(YOR186W_p)|FD-Score:-3.17|P-value:7.66E-4||SGD DESC:Putative protein of unknown function; proper regulation of expression during heat stress is sphingolipid-dependent; YOR186W has a paralog, YLR297W, that arose from the whole genome duplication Gene:YPT31(YER031C)|FD-Score:-4.3|P-value:8.50E-6||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:YUR1(YJL139C)|FD-Score:-3.39|P-value:3.51E-4||SGD DESC:Mannosyltransferase involved in protein N-glycosylation; member of the KTR1 family; located in the Golgi apparatus; YUR1 has a paralog, KTR2, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGR113W5.121.54E-70.65DAM1Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments
YHR062C4.788.72E-70.65RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YLR249W4.751.04E-60.65YEF3Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication
YHR107C4.741.08E-60.65CDC12Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YLR310C4.592.23E-60.65CDC25Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1
YGR030C4.513.20E-60.65POP6Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YKL083W_d4.308.39E-60.65YKL083W_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14
YJL111W4.279.92E-60.65CCT7Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance
YGR198W3.621.48E-40.08YPP1Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene
YDL060W3.541.98E-40.22TSR1Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress
YNL163C3.334.40E-40.10RIA1Cytoplasmic GTPase/eEF2-like factor involved in ribosomal biogenesis; with Sdo1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; required for quality control check of newly made large ribosomal subunits before they are released into the pool of translating ribosomes
YER148W3.226.38E-40.11SPT15TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability
YOL120C3.119.29E-40.04RPL18ARibosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication
YLR088W3.070.001070.39GAA1Subunit of the GPI (glycosylphosphatidylinositol):protein transamidase complex, removes the GPI-anchoring signal and attaches GPI to proteins in the ER
YJL014W2.680.003700.12CCT3Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR127W25.001.96E-138ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YBR068C19.105.05E-82BAP2High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication
YDR392W17.301.74E-67SPT3Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters
YIR023W14.809.55E-50DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YPR060C12.704.61E-37ARO7Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis
YGR063C9.671.99E-22SPT4Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions
YGL148W9.133.37E-20ARO2Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress
YDR340W_d7.921.23E-15YDR340W_dPutative protein of unknown function
YNL274C6.991.38E-12GOR1Glyoxylate reductase; null mutation results in increased biomass after diauxic shift; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress
YJR115W_p6.232.39E-10YJR115W_pPutative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication
YMR166C_p6.001.02E-9YMR166C_pPredicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene
YMR245W_d5.941.42E-9YMR245W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YHL011C5.744.62E-9PRS35-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes
YMR084W_p5.657.92E-9YMR084W_pPutative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1
YOR070C5.501.87E-8GYP1Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion

GO enrichment analysis for SGTC_2976
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.4191.36E-249SGTC_29329016711 44.5 μMChembridge (Drug-like library)71173280.169014
0.3616.88E-181SGTC_1656st012921 11.0 μMTimTec (Natural product derivative library)67328210.241935
0.3582.34E-178SGTC_12263909-7979 35.4 μMChemDiv (Drug-like library)66155100.128205
0.3411.69E-160SGTC_21325317594 122.7 μMChembridge (Fragment library)7945770.0694444SWF1 & branched chain AA biosynthesis
0.3065.21E-128SGTC_486niguldipine 82.0 μMMiscellaneous12360.0918367amide catabolism
0.2934.53E-117SGTC_2607solasodine 47.4 μMMicrosource (Natural product library)67106420.0315789
0.2901.80E-114SGTC_13041165-0502 251.0 μMChemDiv (Drug-like library)7674250.104478
0.2554.02E-88SGTC_5810958-0271 211.0 μMChemDiv (Drug-like library)45911450.115385
0.2481.72E-83SGTC_505gw-5074 63.9 μMICCB bioactive library59242080.0533333
0.2373.35E-76SGTC_6390833-0042 114.0 μMChemDiv (Drug-like library)59789110.283333
0.2176.47E-64SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.101449
0.2091.59E-59SGTC_3371315-0038 354.0 μMChemDiv (Drug-like library)16264370.0581395amide catabolism
0.2037.72E-56SGTC_10583448-5425 97.3 μMChemDiv (Drug-like library)72797050.106061
0.2023.94E-55SGTC_13131220-0047 237.0 μMChemDiv (Drug-like library)35287060.148148
0.1922.44E-50SGTC_253dyclonine 31.2 μMMiscellaneous31800.15942amide catabolism

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2969909140828.7 μM0.4363644286311Chembridge (Drug-like library)254.326923.31812
SGTC_22406690706185.95 μM0.431034805876Chembridge (Fragment library)266.294562.55213
SGTC_20275150920200 μM0.410714786715Chembridge (Fragment library)273.310342.37334
SGTC_2972909361959.92 μM0.39393925236256Chembridge (Drug-like library)300.41853.51513
SGTC_3291912117771.43 μM0.37313417015612Chembridge (Drug-like library)341.794842.95414
SGTC_20975376186200 μM0.372549742755Chembridge (Fragment library)198.263662.55712
SGTC_23246149169200 μM0.3620692888231Chembridge (Fragment library)247.273062.0124
SGTC_7491000-0129128 μM0.351852708470ChemDiv (Drug-like library)257.310943.15423amide catabolism
SGTC_21195528445120.64 μM0.351852791935Chembridge (Fragment library)212.290243.04412
SGTC_3082911700849.47 μM0.32758641448529Chembridge (Drug-like library)240.300342.44112