9022445

3-methoxy-N-(3-propan-2-yloxyphenyl)benzamide

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2986
Screen concentration 71.4 μM
Source Chembridge (Drug-like library)
PubChem CID 6459286
SMILES CC(C)OC1=CC=CC(=C1)NC(=O)C2=CC(=CC=C2)OC
Standardized SMILES COc1cccc(c1)C(=O)Nc2cccc(OC(C)C)c2
Molecular weight 285.3377
ALogP 3.31
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 15.35
% growth inhibition (Hom. pool) 12.17


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6459286
Download HIP data (tab-delimited text)  (excel)
Gene:ARC15(YIL062C)|FD-Score:5.85|P-value:2.41E-9|Clearance:1.43||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; has mRNA binding activity Gene:CCT6(YDR188W)|FD-Score:-3.19|P-value:7.20E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:CDC15(YAR019C)|FD-Score:4.43|P-value:4.75E-6|Clearance:0.07||SGD DESC:Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress Gene:ERG11(YHR007C)|FD-Score:3.31|P-value:4.72E-4|Clearance:0.02||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG27(YLR100W)|FD-Score:3.72|P-value:9.92E-5|Clearance:0.26||SGD DESC:3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs Gene:IPI3(YNL182C)|FD-Score:3.29|P-value:5.00E-4|Clearance:0.1||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; highly conserved protein which contains several WD40 motifs; IPI3 is an essential gene Gene:IRA1(YBR140C)|FD-Score:4.36|P-value:6.45E-6|Clearance:0.17||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:MAS1(YLR163C)|FD-Score:-3.41|P-value:3.26E-4|Clearance:0||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MCM2(YBL023C)|FD-Score:3.19|P-value:7.12E-4|Clearance:0.01||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress Gene:NOP56(YLR197W)|FD-Score:-6.36|P-value:1.01E-10|Clearance:0||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:RER2(YBR002C)|FD-Score:-4.23|P-value:1.18E-5|Clearance:0||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:SEC16(YPL085W)|FD-Score:3.85|P-value:5.97E-5|Clearance:0.13||SGD DESC:COPII vesicle coat protein required for ER transport vesicle budding; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p Gene:SPB4(YFL002C)|FD-Score:-4.21|P-value:1.29E-5|Clearance:0||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:SPN1(YPR133C)|FD-Score:-6.36|P-value:1.02E-10|Clearance:0||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:SPT15(YER148W)|FD-Score:6.19|P-value:2.99E-10|Clearance:1.43||SGD DESC:TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability Gene:SUI2(YJR007W)|FD-Score:-3.95|P-value:3.83E-5|Clearance:0||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TEL2(YGR099W)|FD-Score:-3.46|P-value:2.69E-4|Clearance:0||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1 Gene:TIM17(YJL143W)|FD-Score:3.18|P-value:7.26E-4|Clearance:0.14||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:YAH1(YPL252C)|FD-Score:-3.26|P-value:5.63E-4|Clearance:0||SGD DESC:Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin Gene:YDR526C(YDR526C_d)|FD-Score:4.19|P-value:1.40E-5|Clearance:0.34||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL111C(YKL111C_d)|FD-Score:3.46|P-value:2.70E-4|Clearance:0.15||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene ABF1 Gene:ARC15(YIL062C)|FD-Score:5.85|P-value:2.41E-9|Clearance:1.43||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; has mRNA binding activity Gene:CCT6(YDR188W)|FD-Score:-3.19|P-value:7.20E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:CDC15(YAR019C)|FD-Score:4.43|P-value:4.75E-6|Clearance:0.07||SGD DESC:Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress Gene:ERG11(YHR007C)|FD-Score:3.31|P-value:4.72E-4|Clearance:0.02||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG27(YLR100W)|FD-Score:3.72|P-value:9.92E-5|Clearance:0.26||SGD DESC:3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs Gene:IPI3(YNL182C)|FD-Score:3.29|P-value:5.00E-4|Clearance:0.1||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; highly conserved protein which contains several WD40 motifs; IPI3 is an essential gene Gene:IRA1(YBR140C)|FD-Score:4.36|P-value:6.45E-6|Clearance:0.17||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:MAS1(YLR163C)|FD-Score:-3.41|P-value:3.26E-4|Clearance:0||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MCM2(YBL023C)|FD-Score:3.19|P-value:7.12E-4|Clearance:0.01||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress Gene:NOP56(YLR197W)|FD-Score:-6.36|P-value:1.01E-10|Clearance:0||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:RER2(YBR002C)|FD-Score:-4.23|P-value:1.18E-5|Clearance:0||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:SEC16(YPL085W)|FD-Score:3.85|P-value:5.97E-5|Clearance:0.13||SGD DESC:COPII vesicle coat protein required for ER transport vesicle budding; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p Gene:SPB4(YFL002C)|FD-Score:-4.21|P-value:1.29E-5|Clearance:0||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:SPN1(YPR133C)|FD-Score:-6.36|P-value:1.02E-10|Clearance:0||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:SPT15(YER148W)|FD-Score:6.19|P-value:2.99E-10|Clearance:1.43||SGD DESC:TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability Gene:SUI2(YJR007W)|FD-Score:-3.95|P-value:3.83E-5|Clearance:0||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TEL2(YGR099W)|FD-Score:-3.46|P-value:2.69E-4|Clearance:0||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1 Gene:TIM17(YJL143W)|FD-Score:3.18|P-value:7.26E-4|Clearance:0.14||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:YAH1(YPL252C)|FD-Score:-3.26|P-value:5.63E-4|Clearance:0||SGD DESC:Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin Gene:YDR526C(YDR526C_d)|FD-Score:4.19|P-value:1.40E-5|Clearance:0.34||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL111C(YKL111C_d)|FD-Score:3.46|P-value:2.70E-4|Clearance:0.15||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene ABF1

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6459286
Download HOP data (tab-delimited text)  (excel)
Gene:AAD3(YCR107W)|FD-Score:3.75|P-value:9.00E-5||SGD DESC:Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role Gene:AAT2(YLR027C)|FD-Score:-3.65|P-value:1.34E-4||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:ARP6(YLR085C)|FD-Score:-3.7|P-value:1.10E-4||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:AST2(YER101C)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:Lipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication Gene:CBP1(YJL209W)|FD-Score:3.11|P-value:9.21E-4||SGD DESC:Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress Gene:CCR4(YAL021C)|FD-Score:4.09|P-value:2.19E-5||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:COY1(YKL179C)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function Gene:CWP2(YKL096W-A)|FD-Score:5.85|P-value:2.47E-9||SGD DESC:Covalently linked cell wall mannoprotein, major constituent of the cell wall; plays a role in stabilizing the cell wall; involved in low pH resistance; precursor is GPI-anchored Gene:DYN3(YMR299C)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:ENV10(YLR065C)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:ESBP6(YNL125C)|FD-Score:3.94|P-value:4.14E-5||SGD DESC:Protein with similarity to monocarboxylate permeases, appears not to be involved in transport of monocarboxylates such as lactate, pyruvate or acetate across the plasma membrane Gene:EST2(YLR318W)|FD-Score:-3.12|P-value:8.98E-4||SGD DESC:Reverse transcriptase subunit of the telomerase holoenzyme, essential for telomerase core catalytic activity, involved in other aspects of telomerase assembly and function; mutations in human homolog are associated with aplastic anemia Gene:FAR3(YMR052W)|FD-Score:4.14|P-value:1.76E-5||SGD DESC:Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:GCS1(YDL226C)|FD-Score:6.44|P-value:6.14E-11||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GIP1(YBR045C)|FD-Score:4.48|P-value:3.66E-6||SGD DESC:Meiosis-specific regulatory subunit of the Glc7p protein phosphatase, regulates spore wall formation and septin organization, required for expression of some late meiotic genes and for normal localization of Glc7p Gene:HAP4(YKL109W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex Gene:HCS1(YKL017C)|FD-Score:10.3|P-value:5.21E-25||SGD DESC:Hexameric DNA polymerase alpha-associated DNA helicase A involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities Gene:HES1(YOR237W)|FD-Score:-3.89|P-value:4.91E-5||SGD DESC:Protein implicated in the regulation of ergosterol biosynthesis; one of a seven member gene family with a common essential function and non-essential unique functions; similar to human oxysterol binding protein (OSBP); HES1 has a paralog, KES1, that arose from the whole genome duplication Gene:INM1(YHR046C)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate Gene:MEK1(YOR351C)|FD-Score:3.26|P-value:5.65E-4||SGD DESC:Meiosis-specific serine/threonine protein kinase, functions in meiotic checkpoint, promotes recombination between homologous chromosomes by suppressing double strand break repair between sister chromatids Gene:MIC17(YMR002W)|FD-Score:-4.12|P-value:1.86E-5||SGD DESC:Mitochondrial intermembrane space protein; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MLH1(YMR167W)|FD-Score:3.88|P-value:5.17E-5||SGD DESC:Protein required for mismatch repair in mitosis and meiosis as well as crossing over during meiosis; forms a complex with Pms1p and Msh2p-Msh3p during mismatch repair; human homolog is associated with hereditary non-polyposis colon cancer Gene:MSD1(YPL104W)|FD-Score:-4.27|P-value:9.65E-6||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:MST1(YKL194C)|FD-Score:-4.7|P-value:1.29E-6||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:NEW1(YPL226W)|FD-Score:-3.49|P-value:2.42E-4||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:OTU1(YFL044C)|FD-Score:3.14|P-value:8.46E-4||SGD DESC:Deubiquitylation enzyme that binds to the chaperone-ATPase Cdc48p; may contribute to regulation of protein degradation by deubiquitylating substrates that have been ubiquitylated by Ufd2p; member of the Ovarian Tumor (OTU) family; protein abundance increases in response to DNA replication stress Gene:PET20(YPL159C)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome Gene:PHB2(YGR231C)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation Gene:PPG1(YNR032W)|FD-Score:-3.21|P-value:6.70E-4||SGD DESC:Putative serine/threonine protein phosphatase of the type 2A-like phosphatase family, required for glycogen accumulation; interacts with Tap42p, which binds to and regulates other protein phosphatases Gene:PPT2(YPL148C)|FD-Score:-4.16|P-value:1.58E-5||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PRO2(YOR323C)|FD-Score:4|P-value:3.16E-5||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PSP2(YML017W)|FD-Score:4.74|P-value:1.05E-6||SGD DESC:Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing Gene:PTH1(YHR189W)|FD-Score:3.18|P-value:7.31E-4||SGD DESC:One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium Gene:RAD55(YDR076W)|FD-Score:-3.27|P-value:5.34E-4||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:RAD7(YJR052W)|FD-Score:-3.3|P-value:4.77E-4||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad16p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RAM1(YDL090C)|FD-Score:-3.29|P-value:5.00E-4||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RIF1(YBR275C)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RPL27A(YHR010W)|FD-Score:-3.91|P-value:4.62E-5||SGD DESC:Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication Gene:SCS2(YER120W)|FD-Score:3.16|P-value:7.97E-4||SGD DESC:Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; interacts with FFAT motif of Opi1p; involved in telomeric silencing; null shows inositol auxotrophy above 34 deg C; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication Gene:SDC1(YDR469W)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 Gene:SKG1(YKR100C)|FD-Score:-3.15|P-value:8.12E-4||SGD DESC:Transmembrane protein with a role in cell wall polymer composition; localizes on the inner surface of the plasma membrane at the bud and in the daughter cell Gene:SPP1(YPL138C)|FD-Score:3.77|P-value:8.26E-5||SGD DESC:Subunit of COMPASS (Set1C), a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; interacts with Orc2p; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein Gene:STE20(YHL007C)|FD-Score:5.92|P-value:1.58E-9||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p Gene:SUS1(YBR111W-A)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:SWT1(YOR166C)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:RNA endoribonuclease involved in perinuclear mRNP quality control via the turnover of aberrant, unprocessed pre-mRNAs; interacts with subunits of THO/TREX, TREX-2, and RNA polymerase II; contains a PIN (PilT N terminus) domain Gene:TOS1(YBR162C)|FD-Score:4.47|P-value:3.88E-6||SGD DESC:Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C Gene:TRM3(YDL112W)|FD-Score:-3.8|P-value:7.37E-5||SGD DESC:2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs Gene:VNX1(YNL321W)|FD-Score:3.95|P-value:3.85E-5||SGD DESC:Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate Gene:VPS30(YPL120W)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1 Gene:YAL004W(YAL004W_d)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C Gene:YBL083C(YBL083C_d)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Gene:YCR085W(YCR085W_d)|FD-Score:4.45|P-value:4.36E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR169C-A(YDR169C-A_p)|FD-Score:-3.89|P-value:5.02E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR445C(YDR445C_d)|FD-Score:-4.59|P-value:2.24E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER119C-A(YER119C-A_d)|FD-Score:3.97|P-value:3.59E-5||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YGR219W(YGR219W_d)|FD-Score:-3.43|P-value:2.97E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C Gene:YGR269W(YGR269W_d)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF HUA1/YGR268C Gene:YIL077C(YIL077C_p)|FD-Score:-5.88|P-value:2.08E-9||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YLR311C(YLR311C_d)|FD-Score:-4.58|P-value:2.29E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML003W(YML003W_p)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Putative protein of unknown function Gene:YMR124W(YMR124W_p)|FD-Score:3.81|P-value:7.05E-5||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization Gene:YNL034W(YNL034W_p)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Putative protein of unknown function; YNL034W is not an essential gene Gene:YNL277W-A(YNL277W-A_p)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Putative protein of unknown function Gene:YOR029W(YOR029W_d)|FD-Score:3.1|P-value:9.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR123C(YPR123C_d)|FD-Score:3.77|P-value:8.08E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPT6(YLR262C)|FD-Score:5.93|P-value:1.47E-9||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:ZWF1(YNL241C)|FD-Score:3.19|P-value:7.22E-4||SGD DESC:Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress Gene:AAD3(YCR107W)|FD-Score:3.75|P-value:9.00E-5||SGD DESC:Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role Gene:AAT2(YLR027C)|FD-Score:-3.65|P-value:1.34E-4||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:ARP6(YLR085C)|FD-Score:-3.7|P-value:1.10E-4||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:AST2(YER101C)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:Lipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication Gene:CBP1(YJL209W)|FD-Score:3.11|P-value:9.21E-4||SGD DESC:Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress Gene:CCR4(YAL021C)|FD-Score:4.09|P-value:2.19E-5||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:COY1(YKL179C)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function Gene:CWP2(YKL096W-A)|FD-Score:5.85|P-value:2.47E-9||SGD DESC:Covalently linked cell wall mannoprotein, major constituent of the cell wall; plays a role in stabilizing the cell wall; involved in low pH resistance; precursor is GPI-anchored Gene:DYN3(YMR299C)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:ENV10(YLR065C)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:ESBP6(YNL125C)|FD-Score:3.94|P-value:4.14E-5||SGD DESC:Protein with similarity to monocarboxylate permeases, appears not to be involved in transport of monocarboxylates such as lactate, pyruvate or acetate across the plasma membrane Gene:EST2(YLR318W)|FD-Score:-3.12|P-value:8.98E-4||SGD DESC:Reverse transcriptase subunit of the telomerase holoenzyme, essential for telomerase core catalytic activity, involved in other aspects of telomerase assembly and function; mutations in human homolog are associated with aplastic anemia Gene:FAR3(YMR052W)|FD-Score:4.14|P-value:1.76E-5||SGD DESC:Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:GCS1(YDL226C)|FD-Score:6.44|P-value:6.14E-11||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GIP1(YBR045C)|FD-Score:4.48|P-value:3.66E-6||SGD DESC:Meiosis-specific regulatory subunit of the Glc7p protein phosphatase, regulates spore wall formation and septin organization, required for expression of some late meiotic genes and for normal localization of Glc7p Gene:HAP4(YKL109W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex Gene:HCS1(YKL017C)|FD-Score:10.3|P-value:5.21E-25||SGD DESC:Hexameric DNA polymerase alpha-associated DNA helicase A involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities Gene:HES1(YOR237W)|FD-Score:-3.89|P-value:4.91E-5||SGD DESC:Protein implicated in the regulation of ergosterol biosynthesis; one of a seven member gene family with a common essential function and non-essential unique functions; similar to human oxysterol binding protein (OSBP); HES1 has a paralog, KES1, that arose from the whole genome duplication Gene:INM1(YHR046C)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate Gene:MEK1(YOR351C)|FD-Score:3.26|P-value:5.65E-4||SGD DESC:Meiosis-specific serine/threonine protein kinase, functions in meiotic checkpoint, promotes recombination between homologous chromosomes by suppressing double strand break repair between sister chromatids Gene:MIC17(YMR002W)|FD-Score:-4.12|P-value:1.86E-5||SGD DESC:Mitochondrial intermembrane space protein; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MLH1(YMR167W)|FD-Score:3.88|P-value:5.17E-5||SGD DESC:Protein required for mismatch repair in mitosis and meiosis as well as crossing over during meiosis; forms a complex with Pms1p and Msh2p-Msh3p during mismatch repair; human homolog is associated with hereditary non-polyposis colon cancer Gene:MSD1(YPL104W)|FD-Score:-4.27|P-value:9.65E-6||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:MST1(YKL194C)|FD-Score:-4.7|P-value:1.29E-6||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:NEW1(YPL226W)|FD-Score:-3.49|P-value:2.42E-4||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:OTU1(YFL044C)|FD-Score:3.14|P-value:8.46E-4||SGD DESC:Deubiquitylation enzyme that binds to the chaperone-ATPase Cdc48p; may contribute to regulation of protein degradation by deubiquitylating substrates that have been ubiquitylated by Ufd2p; member of the Ovarian Tumor (OTU) family; protein abundance increases in response to DNA replication stress Gene:PET20(YPL159C)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome Gene:PHB2(YGR231C)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation Gene:PPG1(YNR032W)|FD-Score:-3.21|P-value:6.70E-4||SGD DESC:Putative serine/threonine protein phosphatase of the type 2A-like phosphatase family, required for glycogen accumulation; interacts with Tap42p, which binds to and regulates other protein phosphatases Gene:PPT2(YPL148C)|FD-Score:-4.16|P-value:1.58E-5||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PRO2(YOR323C)|FD-Score:4|P-value:3.16E-5||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PSP2(YML017W)|FD-Score:4.74|P-value:1.05E-6||SGD DESC:Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing Gene:PTH1(YHR189W)|FD-Score:3.18|P-value:7.31E-4||SGD DESC:One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium Gene:RAD55(YDR076W)|FD-Score:-3.27|P-value:5.34E-4||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:RAD7(YJR052W)|FD-Score:-3.3|P-value:4.77E-4||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad16p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RAM1(YDL090C)|FD-Score:-3.29|P-value:5.00E-4||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RIF1(YBR275C)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RPL27A(YHR010W)|FD-Score:-3.91|P-value:4.62E-5||SGD DESC:Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication Gene:SCS2(YER120W)|FD-Score:3.16|P-value:7.97E-4||SGD DESC:Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; interacts with FFAT motif of Opi1p; involved in telomeric silencing; null shows inositol auxotrophy above 34 deg C; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication Gene:SDC1(YDR469W)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 Gene:SKG1(YKR100C)|FD-Score:-3.15|P-value:8.12E-4||SGD DESC:Transmembrane protein with a role in cell wall polymer composition; localizes on the inner surface of the plasma membrane at the bud and in the daughter cell Gene:SPP1(YPL138C)|FD-Score:3.77|P-value:8.26E-5||SGD DESC:Subunit of COMPASS (Set1C), a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; interacts with Orc2p; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein Gene:STE20(YHL007C)|FD-Score:5.92|P-value:1.58E-9||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p Gene:SUS1(YBR111W-A)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:SWT1(YOR166C)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:RNA endoribonuclease involved in perinuclear mRNP quality control via the turnover of aberrant, unprocessed pre-mRNAs; interacts with subunits of THO/TREX, TREX-2, and RNA polymerase II; contains a PIN (PilT N terminus) domain Gene:TOS1(YBR162C)|FD-Score:4.47|P-value:3.88E-6||SGD DESC:Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C Gene:TRM3(YDL112W)|FD-Score:-3.8|P-value:7.37E-5||SGD DESC:2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs Gene:VNX1(YNL321W)|FD-Score:3.95|P-value:3.85E-5||SGD DESC:Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate Gene:VPS30(YPL120W)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1 Gene:YAL004W(YAL004W_d)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C Gene:YBL083C(YBL083C_d)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Gene:YCR085W(YCR085W_d)|FD-Score:4.45|P-value:4.36E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR169C-A(YDR169C-A_p)|FD-Score:-3.89|P-value:5.02E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR445C(YDR445C_d)|FD-Score:-4.59|P-value:2.24E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER119C-A(YER119C-A_d)|FD-Score:3.97|P-value:3.59E-5||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YGR219W(YGR219W_d)|FD-Score:-3.43|P-value:2.97E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C Gene:YGR269W(YGR269W_d)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF HUA1/YGR268C Gene:YIL077C(YIL077C_p)|FD-Score:-5.88|P-value:2.08E-9||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YLR311C(YLR311C_d)|FD-Score:-4.58|P-value:2.29E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML003W(YML003W_p)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Putative protein of unknown function Gene:YMR124W(YMR124W_p)|FD-Score:3.81|P-value:7.05E-5||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization Gene:YNL034W(YNL034W_p)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Putative protein of unknown function; YNL034W is not an essential gene Gene:YNL277W-A(YNL277W-A_p)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Putative protein of unknown function Gene:YOR029W(YOR029W_d)|FD-Score:3.1|P-value:9.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR123C(YPR123C_d)|FD-Score:3.77|P-value:8.08E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPT6(YLR262C)|FD-Score:5.93|P-value:1.47E-9||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:ZWF1(YNL241C)|FD-Score:3.19|P-value:7.22E-4||SGD DESC:Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YER148W6.192.99E-101.43SPT15TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability
YIL062C5.852.41E-91.43ARC15Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; has mRNA binding activity
YAR019C4.434.75E-60.07CDC15Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress
YBR140C4.366.45E-60.17IRA1GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication
YDR526C_d4.191.40E-50.34YDR526C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YPL085W3.855.97E-50.13SEC16COPII vesicle coat protein required for ER transport vesicle budding; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p
YLR100W3.729.92E-50.26ERG273-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs
YKL111C_d3.462.70E-40.15YKL111C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene ABF1
YHR007C3.314.72E-40.02ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YNL182C3.295.00E-40.10IPI3Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; highly conserved protein which contains several WD40 motifs; IPI3 is an essential gene
YBL023C3.197.12E-40.01MCM2Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress
YJL143W3.187.26E-40.14TIM17Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex
YJR022W3.050.001150.07LSM8Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) that is part of spliceosomal U6 snRNP and is also implicated in processing of pre-tRNA, pre-snoRNA, and pre-rRNA
YBR254C2.980.001430.00TRS20One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder
YGR156W2.980.001450.06PTI1Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL017C10.305.21E-25HCS1Hexameric DNA polymerase alpha-associated DNA helicase A involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities
YDL226C6.446.14E-11GCS1ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p
YLR262C5.931.47E-9YPT6Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6
YHL007C5.921.58E-9STE20Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p
YKL096W-A5.852.47E-9CWP2Covalently linked cell wall mannoprotein, major constituent of the cell wall; plays a role in stabilizing the cell wall; involved in low pH resistance; precursor is GPI-anchored
YML017W4.741.05E-6PSP2Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing
YBR045C4.483.66E-6GIP1Meiosis-specific regulatory subunit of the Glc7p protein phosphatase, regulates spore wall formation and septin organization, required for expression of some late meiotic genes and for normal localization of Glc7p
YBR162C4.473.88E-6TOS1Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C
YCR085W_d4.454.36E-6YCR085W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YER101C4.151.66E-5AST2Lipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication
YMR052W4.141.76E-5FAR3Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
YAL021C4.092.19E-5CCR4Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening
YOR323C4.003.16E-5PRO2Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis
YER119C-A_d3.973.59E-5YER119C-A_dDubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2
YNL321W3.953.85E-5VNX1Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate

GO enrichment analysis for SGTC_2986
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1155.62E-19SGTC_8750709-0210 25.7 μMChemDiv (Drug-like library)62513640.106061fatty acid desaturase (OLE1)
0.1132.87E-18SGTC_14224031-0024 15.5 μMChemDiv (Drug-like library)7824270.229508iron homeostasis
0.0992.02E-14SGTC_31619100388 49.5 μMChembridge (Drug-like library)252363680.142857iron homeostasis
0.0983.52E-14SGTC_29919074669 71.4 μMChembridge (Drug-like library)173331090.08azole & statin
0.0881.06E-11SGTC_2721ifenprodil 61.4 μMMiscellaneous36890.101449plasma membrane duress
0.0881.56E-11SGTC_218alverine citrate 93.8 μMMiscellaneous217180.0909091fatty acid desaturase (OLE1)
0.0863.60E-11SGTC_20505263056 171.0 μMChembridge (Fragment library)7634680.0645161fatty acid desaturase (OLE1)
0.0864.54E-11SGTC_384k007-0144 5.2 μMChemDiv (Drug-like library)68248860.212121ERAD & cell cycle
0.0822.83E-10SGTC_29779011197 71.4 μMChembridge (Drug-like library)27047220.245614
0.0823.42E-10SGTC_31889110789 49.5 μMChembridge (Drug-like library)8492170.5iron homeostasis
0.0815.41E-10SGTC_29839022671 13.0 μMChembridge (Drug-like library)64051610.13580260S ribosome export
0.0799.95E-10SGTC_1731st035964 63.0 μMTimTec (Natural product derivative library)67395750.147059
0.0781.94E-9SGTC_8680527-0199 213.0 μMChemDiv (Drug-like library)28494500.144928fatty acid desaturase (OLE1)
0.0772.62E-9SGTC_1855st056195 39.6 μMTimTec (Natural product derivative library)40283790.094594660S ribosome export
0.0772.63E-9SGTC_13131220-0047 237.0 μMChemDiv (Drug-like library)35287060.137255

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2877904072471.43 μM0.8048786463895Chembridge (Drug-like library)285.337663.30813
SGTC_2999906976571.43 μM0.8048782097066Chembridge (Drug-like library)285.337663.30813amide catabolism
SGTC_3077911731949.47 μM0.80487817154678Chembridge (Drug-like library)285.337663.30813
SGTC_3070911700549.47 μM0.6818188779016Chembridge (Drug-like library)273.3021433.5313
SGTC_5544483-2211187 μM0.674419747107ChemDiv (Drug-like library)285.29462.45314
SGTC_2900532852885.29 μM0.674419795359Chembridge (Drug-like library)261.703583.26212Golgi
SGTC_2827799384771.43 μM0.6252985692Chembridge (Drug-like library)283.321783.19813
SGTC_3205911348349.47 μM0.60869617018033Chembridge (Drug-like library)271.311083.06813
SGTC_2851901592271.43 μM0.6041672996893Chembridge (Drug-like library)299.364243.31513
SGTC_2865903031351.95 μM0.5490242562754Chembridge (Drug-like library)295.332483.91913