9054257

2-[4-[(4-chlorophenyl)methyl]piperazin-1-yl]-N-phenylacetamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2993
Screen concentration 71.4 μM
Source Chembridge (Drug-like library)
PubChem CID 6470217
SMILES C1CN(CCN1CC2=CC=C(C=C2)Cl)CC(=O)NC3=CC=CC=C3
Standardized SMILES Clc1ccc(CN2CCN(CC(=O)Nc3ccccc3)CC2)cc1
Molecular weight 343.8505
ALogP 3.16
H-bond donor count 1
H-bond acceptor count 3
Response signature fatty acid desaturase (OLE1)

Pool Growth Kinetics
% growth inhibition (Het. pool) 19.21
% growth inhibition (Hom. pool) 10.4


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6470217
Download HIP data (tab-delimited text)  (excel)
Gene:ATM1(YMR301C)|FD-Score:3.4|P-value:3.39E-4|Clearance:0.03||SGD DESC:Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol Gene:CCT4(YDL143W)|FD-Score:6.54|P-value:3.02E-11|Clearance:0.39||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT5(YJR064W)|FD-Score:3.63|P-value:1.43E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC20(YGL116W)|FD-Score:6.7|P-value:1.07E-11|Clearance:0.15||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:COP1(YDL145C)|FD-Score:4.48|P-value:3.67E-6|Clearance:0.12||SGD DESC:Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway Gene:FCF2(YLR051C)|FD-Score:4.74|P-value:1.05E-6|Clearance:0.08||SGD DESC:Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon Gene:GFA1(YKL104C)|FD-Score:3.42|P-value:3.18E-4|Clearance:0.02||SGD DESC:Glutamine-fructose-6-phosphate amidotransferase; catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis; GFA1 has a paralogous region, comprising ORFs YMR084W-YMR085W, that arose from the whole genome duplication Gene:GPI11(YDR302W)|FD-Score:3.88|P-value:5.20E-5|Clearance:0.09||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:HEM12(YDR047W)|FD-Score:4.99|P-value:3.09E-7|Clearance:0.05||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:IRA1(YBR140C)|FD-Score:3.54|P-value:2.04E-4|Clearance:0.02||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:KAP95(YLR347C)|FD-Score:3.24|P-value:6.07E-4|Clearance:0.06||SGD DESC:Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p Gene:LSG1(YGL099W)|FD-Score:5.97|P-value:1.18E-9|Clearance:0.07||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MCD4(YKL165C)|FD-Score:5.51|P-value:1.76E-8|Clearance:0.41||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:MES1(YGR264C)|FD-Score:-3.54|P-value:2.02E-4|Clearance:0||SGD DESC:Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs Gene:MPS3(YJL019W)|FD-Score:-4.28|P-value:9.15E-6|Clearance:0||SGD DESC:Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p Gene:NIP1(YMR309C)|FD-Score:3.5|P-value:2.36E-4|Clearance:0.05||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:NMD3(YHR170W)|FD-Score:4.36|P-value:6.42E-6|Clearance:0.12||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOC2(YOR206W)|FD-Score:6.1|P-value:5.24E-10|Clearance:0.01||SGD DESC:Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors Gene:NOG1(YPL093W)|FD-Score:3.79|P-value:7.48E-5|Clearance:0.03||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:NOP8(YOL144W)|FD-Score:12.8|P-value:7.07E-38|Clearance:2.24||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:OLE1(YGL055W)|FD-Score:19.6|P-value:7.02E-86|Clearance:6.79||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PDI1(YCL043C)|FD-Score:-4.62|P-value:1.89E-6|Clearance:0||SGD DESC:Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication Gene:PIS1(YPR113W)|FD-Score:7.26|P-value:1.95E-13|Clearance:0.09||SGD DESC:Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins Gene:PKC1(YBL105C)|FD-Score:6.16|P-value:3.70E-10|Clearance:0.05||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:POL30(YBR088C)|FD-Score:3.3|P-value:4.85E-4|Clearance:0.06||SGD DESC:Proliferating cell nuclear antigen (PCNA), functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair Gene:PRE5(YMR314W)|FD-Score:-4.43|P-value:4.78E-6|Clearance:0||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:RAT1(YOR048C)|FD-Score:-3.28|P-value:5.23E-4|Clearance:0||SGD DESC:Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination Gene:RER2(YBR002C)|FD-Score:-5.48|P-value:2.08E-8|Clearance:0||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RET1(YOR207C)|FD-Score:-4.39|P-value:5.79E-6|Clearance:0||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RLP24(YLR009W)|FD-Score:6.92|P-value:2.26E-12|Clearance:0.21||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPA190(YOR341W)|FD-Score:3.11|P-value:9.38E-4|Clearance:0.05||SGD DESC:RNA polymerase I largest subunit A190 Gene:RPL10(YLR075W)|FD-Score:3.55|P-value:1.91E-4|Clearance:0.02||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPL18A(YOL120C)|FD-Score:7.17|P-value:3.74E-13|Clearance:0.25||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPL28(YGL103W)|FD-Score:4.59|P-value:2.24E-6|Clearance:0.01||SGD DESC:Ribosomal 60S subunit protein L28; homologous to mammalian ribosomal protein L27A and bacterial L15; may have peptidyl transferase activity; can mutate to cycloheximide resistance Gene:RPL30(YGL030W)|FD-Score:3.44|P-value:2.87E-4|Clearance:0.03||SGD DESC:Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog Gene:RPL33A(YPL143W)|FD-Score:3.4|P-value:3.39E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication Gene:RPL5(YPL131W)|FD-Score:4.24|P-value:1.12E-5|Clearance:0.18||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RPS15(YOL040C)|FD-Score:3.62|P-value:1.45E-4|Clearance:0.06||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RRP43(YCR035C)|FD-Score:4.58|P-value:2.35E-6|Clearance:0.09||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress Gene:RSC4(YKR008W)|FD-Score:3.72|P-value:9.94E-5|Clearance:0.09||SGD DESC:Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p Gene:RSC6(YCR052W)|FD-Score:3.98|P-value:3.41E-5|Clearance:0.1||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC9(YML127W)|FD-Score:3.36|P-value:3.85E-4|Clearance:0.03||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SAR1(YPL218W)|FD-Score:-3.3|P-value:4.91E-4|Clearance:0||SGD DESC:GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport Gene:SEC13(YLR208W)|FD-Score:-4.97|P-value:3.39E-7|Clearance:0||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC21(YNL287W)|FD-Score:5.71|P-value:5.63E-9|Clearance:0.2||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SFT1(YKL006C-A)|FD-Score:-3.14|P-value:8.58E-4|Clearance:0||SGD DESC:Intra-Golgi v-SNARE, required for transport of proteins between an early and a later Golgi compartment Gene:SUA7(YPR086W)|FD-Score:3.33|P-value:4.28E-4|Clearance:0.04||SGD DESC:Transcription factor TFIIB, a general transcription factor required for transcription initiation and start site selection by RNA polymerase II Gene:SUI2(YJR007W)|FD-Score:6.1|P-value:5.45E-10|Clearance:0.07||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TCP1(YDR212W)|FD-Score:6.1|P-value:5.19E-10|Clearance:0||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:TIF6(YPR016C)|FD-Score:4.66|P-value:1.58E-6|Clearance:0.07||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Gene:TIM23(YNR017W)|FD-Score:4.06|P-value:2.49E-5|Clearance:0.07||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel Gene:TOM40(YMR203W)|FD-Score:6.03|P-value:8.39E-10|Clearance:0.06||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TSR1(YDL060W)|FD-Score:-4.21|P-value:1.30E-5|Clearance:0||SGD DESC:Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:TUB1(YML085C)|FD-Score:-3.73|P-value:9.40E-5|Clearance:0||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:YBR190W(YBR190W_d)|FD-Score:4.93|P-value:4.07E-7|Clearance:0.19||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YDL196W(YDL196W_d)|FD-Score:6.71|P-value:9.43E-12|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YDR187C(YDR187C_d)|FD-Score:3.18|P-value:7.38E-4|Clearance:0.07||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YDR526C(YDR526C_d)|FD-Score:3.56|P-value:1.84E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR115C(YGR115C_d)|FD-Score:-4.39|P-value:5.75E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YJL009W(YJL009W_d)|FD-Score:3.51|P-value:2.20E-4|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex Gene:YJL086C(YJL086C_d)|FD-Score:5.9|P-value:1.83E-9|Clearance:0.19||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YJL195C(YJL195C_d)|FD-Score:10.6|P-value:1.94E-26|Clearance:3.32||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YPL142C(YPL142C_d)|FD-Score:3.76|P-value:8.37E-5|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit Gene:YPL238C(YPL238C_d)|FD-Score:5.1|P-value:1.67E-7|Clearance:0.12||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:ATM1(YMR301C)|FD-Score:3.4|P-value:3.39E-4|Clearance:0.03||SGD DESC:Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol Gene:CCT4(YDL143W)|FD-Score:6.54|P-value:3.02E-11|Clearance:0.39||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT5(YJR064W)|FD-Score:3.63|P-value:1.43E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC20(YGL116W)|FD-Score:6.7|P-value:1.07E-11|Clearance:0.15||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:COP1(YDL145C)|FD-Score:4.48|P-value:3.67E-6|Clearance:0.12||SGD DESC:Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway Gene:FCF2(YLR051C)|FD-Score:4.74|P-value:1.05E-6|Clearance:0.08||SGD DESC:Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon Gene:GFA1(YKL104C)|FD-Score:3.42|P-value:3.18E-4|Clearance:0.02||SGD DESC:Glutamine-fructose-6-phosphate amidotransferase; catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis; GFA1 has a paralogous region, comprising ORFs YMR084W-YMR085W, that arose from the whole genome duplication Gene:GPI11(YDR302W)|FD-Score:3.88|P-value:5.20E-5|Clearance:0.09||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:HEM12(YDR047W)|FD-Score:4.99|P-value:3.09E-7|Clearance:0.05||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:IRA1(YBR140C)|FD-Score:3.54|P-value:2.04E-4|Clearance:0.02||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:KAP95(YLR347C)|FD-Score:3.24|P-value:6.07E-4|Clearance:0.06||SGD DESC:Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p Gene:LSG1(YGL099W)|FD-Score:5.97|P-value:1.18E-9|Clearance:0.07||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MCD4(YKL165C)|FD-Score:5.51|P-value:1.76E-8|Clearance:0.41||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:MES1(YGR264C)|FD-Score:-3.54|P-value:2.02E-4|Clearance:0||SGD DESC:Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs Gene:MPS3(YJL019W)|FD-Score:-4.28|P-value:9.15E-6|Clearance:0||SGD DESC:Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p Gene:NIP1(YMR309C)|FD-Score:3.5|P-value:2.36E-4|Clearance:0.05||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:NMD3(YHR170W)|FD-Score:4.36|P-value:6.42E-6|Clearance:0.12||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOC2(YOR206W)|FD-Score:6.1|P-value:5.24E-10|Clearance:0.01||SGD DESC:Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors Gene:NOG1(YPL093W)|FD-Score:3.79|P-value:7.48E-5|Clearance:0.03||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:NOP8(YOL144W)|FD-Score:12.8|P-value:7.07E-38|Clearance:2.24||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:OLE1(YGL055W)|FD-Score:19.6|P-value:7.02E-86|Clearance:6.79||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PDI1(YCL043C)|FD-Score:-4.62|P-value:1.89E-6|Clearance:0||SGD DESC:Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication Gene:PIS1(YPR113W)|FD-Score:7.26|P-value:1.95E-13|Clearance:0.09||SGD DESC:Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins Gene:PKC1(YBL105C)|FD-Score:6.16|P-value:3.70E-10|Clearance:0.05||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:POL30(YBR088C)|FD-Score:3.3|P-value:4.85E-4|Clearance:0.06||SGD DESC:Proliferating cell nuclear antigen (PCNA), functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair Gene:PRE5(YMR314W)|FD-Score:-4.43|P-value:4.78E-6|Clearance:0||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:RAT1(YOR048C)|FD-Score:-3.28|P-value:5.23E-4|Clearance:0||SGD DESC:Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination Gene:RER2(YBR002C)|FD-Score:-5.48|P-value:2.08E-8|Clearance:0||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RET1(YOR207C)|FD-Score:-4.39|P-value:5.79E-6|Clearance:0||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RLP24(YLR009W)|FD-Score:6.92|P-value:2.26E-12|Clearance:0.21||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPA190(YOR341W)|FD-Score:3.11|P-value:9.38E-4|Clearance:0.05||SGD DESC:RNA polymerase I largest subunit A190 Gene:RPL10(YLR075W)|FD-Score:3.55|P-value:1.91E-4|Clearance:0.02||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPL18A(YOL120C)|FD-Score:7.17|P-value:3.74E-13|Clearance:0.25||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPL28(YGL103W)|FD-Score:4.59|P-value:2.24E-6|Clearance:0.01||SGD DESC:Ribosomal 60S subunit protein L28; homologous to mammalian ribosomal protein L27A and bacterial L15; may have peptidyl transferase activity; can mutate to cycloheximide resistance Gene:RPL30(YGL030W)|FD-Score:3.44|P-value:2.87E-4|Clearance:0.03||SGD DESC:Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog Gene:RPL33A(YPL143W)|FD-Score:3.4|P-value:3.39E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication Gene:RPL5(YPL131W)|FD-Score:4.24|P-value:1.12E-5|Clearance:0.18||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RPS15(YOL040C)|FD-Score:3.62|P-value:1.45E-4|Clearance:0.06||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RRP43(YCR035C)|FD-Score:4.58|P-value:2.35E-6|Clearance:0.09||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress Gene:RSC4(YKR008W)|FD-Score:3.72|P-value:9.94E-5|Clearance:0.09||SGD DESC:Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p Gene:RSC6(YCR052W)|FD-Score:3.98|P-value:3.41E-5|Clearance:0.1||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC9(YML127W)|FD-Score:3.36|P-value:3.85E-4|Clearance:0.03||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SAR1(YPL218W)|FD-Score:-3.3|P-value:4.91E-4|Clearance:0||SGD DESC:GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport Gene:SEC13(YLR208W)|FD-Score:-4.97|P-value:3.39E-7|Clearance:0||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC21(YNL287W)|FD-Score:5.71|P-value:5.63E-9|Clearance:0.2||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SFT1(YKL006C-A)|FD-Score:-3.14|P-value:8.58E-4|Clearance:0||SGD DESC:Intra-Golgi v-SNARE, required for transport of proteins between an early and a later Golgi compartment Gene:SUA7(YPR086W)|FD-Score:3.33|P-value:4.28E-4|Clearance:0.04||SGD DESC:Transcription factor TFIIB, a general transcription factor required for transcription initiation and start site selection by RNA polymerase II Gene:SUI2(YJR007W)|FD-Score:6.1|P-value:5.45E-10|Clearance:0.07||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TCP1(YDR212W)|FD-Score:6.1|P-value:5.19E-10|Clearance:0||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:TIF6(YPR016C)|FD-Score:4.66|P-value:1.58E-6|Clearance:0.07||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Gene:TIM23(YNR017W)|FD-Score:4.06|P-value:2.49E-5|Clearance:0.07||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel Gene:TOM40(YMR203W)|FD-Score:6.03|P-value:8.39E-10|Clearance:0.06||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TSR1(YDL060W)|FD-Score:-4.21|P-value:1.30E-5|Clearance:0||SGD DESC:Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:TUB1(YML085C)|FD-Score:-3.73|P-value:9.40E-5|Clearance:0||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:YBR190W(YBR190W_d)|FD-Score:4.93|P-value:4.07E-7|Clearance:0.19||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YDL196W(YDL196W_d)|FD-Score:6.71|P-value:9.43E-12|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YDR187C(YDR187C_d)|FD-Score:3.18|P-value:7.38E-4|Clearance:0.07||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YDR526C(YDR526C_d)|FD-Score:3.56|P-value:1.84E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR115C(YGR115C_d)|FD-Score:-4.39|P-value:5.75E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YJL009W(YJL009W_d)|FD-Score:3.51|P-value:2.20E-4|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex Gene:YJL086C(YJL086C_d)|FD-Score:5.9|P-value:1.83E-9|Clearance:0.19||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YJL195C(YJL195C_d)|FD-Score:10.6|P-value:1.94E-26|Clearance:3.32||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YPL142C(YPL142C_d)|FD-Score:3.76|P-value:8.37E-5|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit Gene:YPL238C(YPL238C_d)|FD-Score:5.1|P-value:1.67E-7|Clearance:0.12||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6470217
Download HOP data (tab-delimited text)  (excel)
Gene:AIM17(YHL021C)|FD-Score:-3.61|P-value:1.55E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:AKR1(YDR264C)|FD-Score:3.13|P-value:8.60E-4||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:ALE1(YOR175C)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Broad-specificity lysophospholipid acyltransferase, part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids Gene:APM1(YPL259C)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting Gene:APN1(YKL114C)|FD-Score:3.91|P-value:4.71E-5||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ARO1(YDR127W)|FD-Score:5.63|P-value:8.93E-9||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATG12(YBR217W)|FD-Score:5.81|P-value:3.15E-9||SGD DESC:Conserved ubiquitin-like modifier involved in autophagy and the Cvt pathway; conjugated to Atg5p to form a complex involved in Atg8p lipidation; Atg12p-Atg5p also forms a complex with Atg16p that is required for autophagosome formation Gene:ATP5(YDR298C)|FD-Score:-3.37|P-value:3.79E-4||SGD DESC:Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated Gene:BDH2(YAL061W_p)|FD-Score:-3.35|P-value:4.06E-4||SGD DESC:Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3 Gene:BNI4(YNL233W)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:BUB2(YMR055C)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Mitotic exit network regulator, forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage Gene:CBF1(YJR060W)|FD-Score:8.93|P-value:2.12E-19||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:CCM1(YGR150C)|FD-Score:-3.19|P-value:7.18E-4||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CDC50(YCR094W)|FD-Score:8.28|P-value:6.42E-17||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CHS5(YLR330W)|FD-Score:5.66|P-value:7.37E-9||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:CHS7(YHR142W)|FD-Score:4.65|P-value:1.65E-6||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:CLC1(YGR167W)|FD-Score:-6.96|P-value:1.68E-12||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COG7(YGL005C)|FD-Score:-6.13|P-value:4.36E-10||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ1(YBR003W)|FD-Score:3.11|P-value:9.46E-4||SGD DESC:Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis Gene:CPR7(YJR032W)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity Gene:CTF3(YLR381W)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Outer kinetochore protein that forms a complex with Mcm16p and Mcm22p; may bind the kinetochore to spindle microtubules; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-I and fission yeast mis6 Gene:CYS4(YGR155W)|FD-Score:7.41|P-value:6.48E-14||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DEG1(YFL001W)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability Gene:DFG16(YOR030W)|FD-Score:4.44|P-value:4.58E-6||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DUR1,2(YBR208C)|FD-Score:5.73|P-value:4.99E-9||SGD DESC:Urea amidolyase; contains both urea carboxylase and allophanate hydrolase activities, degrades urea to CO2 and NH3; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation; protein abundance increases in response to DNA replication stress Gene:ERG2(YMR202W)|FD-Score:-3.59|P-value:1.67E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERJ5(YFR041C)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:Type I membrane protein with a J domain is required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response Gene:FAA3(YIL009W)|FD-Score:3.74|P-value:9.21E-5||SGD DESC:Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:FCY1(YPR062W)|FD-Score:3.31|P-value:4.67E-4||SGD DESC:Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) Gene:FPR1(YNL135C)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function Gene:FRE6(YLL051C)|FD-Score:3.33|P-value:4.34E-4||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels Gene:GCG1(YER163C_p)|FD-Score:3.83|P-value:6.51E-5||SGD DESC:Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle Gene:GCN2(YDR283C)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCS1(YDL226C)|FD-Score:24.3|P-value:1.41E-130||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GYP1(YOR070C)|FD-Score:4.04|P-value:2.62E-5||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:IES6(YEL044W)|FD-Score:4.32|P-value:7.88E-6||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes Gene:IRS4(YKR019C)|FD-Score:-5.36|P-value:4.18E-8||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISW1(YBR245C)|FD-Score:4.49|P-value:3.49E-6||SGD DESC:ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p (Isw1a), and a complex with Ioc2p and Ioc4p (Isw1b); Isw1a and Isw1b have partially overlapping and distinct roles, Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation; Isw1b recruited to open reading frames by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions Gene:ISY1(YJR050W)|FD-Score:-3.5|P-value:2.34E-4||SGD DESC:Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles Gene:KCS1(YDR017C)|FD-Score:-3.49|P-value:2.44E-4||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response and telomere maintenance Gene:LCB4(YOR171C)|FD-Score:4.46|P-value:4.08E-6||SGD DESC:Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes Gene:MDY2(YOL111C)|FD-Score:11.7|P-value:5.73E-32||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MIG1(YGL035C)|FD-Score:3.33|P-value:4.34E-4||SGD DESC:Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion Gene:MKK2(YPL140C)|FD-Score:3.83|P-value:6.33E-5||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk1p; MKK2 has a paralog, MKK1, that arose from the whole genome duplication Gene:MMR1(YLR190W)|FD-Score:9.68|P-value:1.79E-22||SGD DESC:Phosphorylated protein of the mitochondrial outer membrane, localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p Gene:MNL1(YHR204W)|FD-Score:4.34|P-value:7.19E-6||SGD DESC:Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation Gene:MRI1(YPR118W)|FD-Score:3.19|P-value:7.14E-4||SGD DESC:5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway Gene:MSL1(YIR009W)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:U2B component of U2 snRNP, involved in splicing, binds the U2 snRNA stem-loop IV in vitro but requires association of Lea1p for in vivo binding; does not contain the conserved C-terminal RNA binding domain found in other family members Gene:MSN1(YOL116W)|FD-Score:4.4|P-value:5.39E-6||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:NOT3(YIL038C)|FD-Score:4.02|P-value:2.95E-5||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:NUT1(YGL151W)|FD-Score:-3.83|P-value:6.29E-5||SGD DESC:Component of the RNA polymerase II mediator complex, which is required for transcriptional activation and also has a role in basal transcription Gene:PAM1(YDR251W)|FD-Score:-3.26|P-value:5.51E-4||SGD DESC:Essential protein of unknown function; exhibits variable expression during colony morphogenesis; overexpression permits survival without protein phosphatase 2A, inhibits growth, and induces a filamentous phenotype Gene:PGC1(YPL206C)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Phosphatidyl Glycerol phospholipase C; regulates the phosphatidylglycerol (PG) content via a phospholipase C-type degradation mechanism; contains glycerophosphodiester phosphodiesterase motifs Gene:PIH1(YHR034C)|FD-Score:3.28|P-value:5.15E-4||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:PLB2(YMR006C)|FD-Score:-3.72|P-value:9.85E-5||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; displays transacylase activity in vitro; overproduction confers resistance to lysophosphatidylcholine Gene:PPT2(YPL148C)|FD-Score:-3.49|P-value:2.41E-4||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PUF3(YLL013C)|FD-Score:7.31|P-value:1.31E-13||SGD DESC:Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins Gene:RAD54(YGL163C)|FD-Score:3.23|P-value:6.13E-4||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:RAI1(YGL246C)|FD-Score:-5.03|P-value:2.45E-7||SGD DESC:Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z Gene:RCY1(YJL204C)|FD-Score:29.1|P-value:5.80E-187||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RGP1(YDR137W)|FD-Score:-8.46|P-value:1.33E-17||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIC1(YLR039C)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RIM8(YGL045W)|FD-Score:4.3|P-value:8.46E-6||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RIM9(YMR063W)|FD-Score:8.86|P-value:3.84E-19||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:ROY1(YMR258C)|FD-Score:4.31|P-value:8.14E-6||SGD DESC:GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p Gene:RPA34(YJL148W)|FD-Score:-3.22|P-value:6.37E-4||SGD DESC:RNA polymerase I subunit A34.5 Gene:RPL22A(YLR061W)|FD-Score:-5.75|P-value:4.36E-9||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPL35B(YDL136W)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RPS11A(YDR025W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication Gene:RPS27A(YKL156W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27A has a paralog, RPS27B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPS9B(YBR189W)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9B has a paralog, RPS9A, that arose from the whole genome duplication Gene:RRT6(YGL146C_p)|FD-Score:-4.06|P-value:2.48E-5||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; contains two putative transmembrane spans, but no significant homology to other known proteins Gene:SBA1(YKL117W)|FD-Score:-3.4|P-value:3.37E-4||SGD DESC:Co-chaperone that binds to and regulates Hsp90 family chaperones; important for pp60v-src activity in yeast; homologous to the mammalian p23 proteins, and like p23 can regulate telomerase activity; protein abundance increases in response to DNA replication stress Gene:SEC22(YLR268W)|FD-Score:3.2|P-value:6.94E-4||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SHE4(YOR035C)|FD-Score:4.36|P-value:6.37E-6||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SIA1(YOR137C)|FD-Score:4.62|P-value:1.95E-6||SGD DESC:Protein of unassigned function involved in activation of the Pma1p plasma membrane H+-ATPase by glucose Gene:SIN3(YOL004W)|FD-Score:4.76|P-value:9.62E-7||SGD DESC:Component of the Sin3p-Rpd3p histone deacetylase complex, involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity Gene:SLG1(YOR008C)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SNT309(YPR101W)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:STI1(YOR027W)|FD-Score:-3.09|P-value:9.85E-4||SGD DESC:Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop Gene:SVP26(YHR181W)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:Integral membrane protein of the early Golgi apparatus and endoplasmic reticulum, involved in COP II vesicle transport; may also function to promote retention of proteins in the early Golgi compartment Gene:SWA2(YDR320C)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:TDA3(YHR009C_p)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Putative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1 Gene:TOM1(YDR457W)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:TRM7(YBR061C)|FD-Score:-4.45|P-value:4.36E-6||SGD DESC:2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively Gene:TRP1(YDR007W)|FD-Score:3.94|P-value:4.08E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:7|P-value:1.25E-12||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:6.99|P-value:1.33E-12||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:21.4|P-value:9.22E-102||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TVP18(YMR071C)|FD-Score:6.15|P-value:3.83E-10||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:UBC12(YLR306W)|FD-Score:-3.41|P-value:3.21E-4||SGD DESC:Enzyme that mediates the conjugation of Rub1p, a ubiquitin-like protein, to other proteins; related to E2 ubiquitin-conjugating enzymes Gene:UBX6(YJL048C)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p, transcription is repressed when cells are grown in media containing inositol and choline; UBX6 has a paralog, UBX7, that arose from the whole genome duplication Gene:VPS20(YMR077C)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS61(YDR136C_d)|FD-Score:-6.17|P-value:3.33E-10||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:-4.54|P-value:2.76E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:VPS68(YOL129W)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria Gene:VPS71(YML041C)|FD-Score:-3.58|P-value:1.73E-4||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:VTA1(YLR181C)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Multivesicular body (MVB) protein involved in endosomal protein sorting; regulates Vps4p activity by promoting its oligomerization; has an N-terminal Vps60- and Did2- binding domain, a linker region, and a C-terminal Vps4p binding domain Gene:YCL001W-A(YCL001W-A_p)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YCR064C(YCR064C_d)|FD-Score:-5.38|P-value:3.68E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YDL011C(YDL011C_d)|FD-Score:-3.2|P-value:6.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YDL010W Gene:YDR008C(YDR008C_d)|FD-Score:14.2|P-value:6.53E-46||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR179W-A(YDR179W-A_p)|FD-Score:4.32|P-value:7.93E-6||SGD DESC:Putative protein of unknown function Gene:YDR282C(YDR282C_p)|FD-Score:3.86|P-value:5.78E-5||SGD DESC:Putative protein of unknown function; localizes to the inner mitochondrial membrane with the C terminus facing the intermembrane space; ortholog of human RMND1, mutation in which is implicated in infantile encephaloneuromyopathy and defective mitochondrial translation Gene:YEL1(YBL060W)|FD-Score:3.87|P-value:5.53E-5||SGD DESC:Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip Gene:YER084W(YER084W_p)|FD-Score:5.7|P-value:6.13E-9||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YFL040W(YFL040W_p)|FD-Score:-3.1|P-value:9.52E-4||SGD DESC:Putative transporter, member of the sugar porter family; YFL040W is not an essential gene Gene:YGL082W(YGL082W_p)|FD-Score:-3.24|P-value:5.89E-4||SGD DESC:Putative protein of unknown function; predicted prenylation/proteolysis target of Afc1p and Rce1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YGL082W is not an essential gene Gene:YGL159W(YGL159W_p)|FD-Score:4.64|P-value:1.77E-6||SGD DESC:Putative protein of unknown function; deletion mutant has no detectable phenotype Gene:YGR122W(YGR122W)|FD-Score:6.09|P-value:5.69E-10||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YHL037C(YHL037C_d)|FD-Score:3.77|P-value:8.01E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL001W(YIL001W_p)|FD-Score:-3.48|P-value:2.55E-4||SGD DESC:Putative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm Gene:YJL119C(YJL119C_d)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL144W(YJL144W)|FD-Score:-3.44|P-value:2.87E-4||SGD DESC:Cytoplasmic hydrophilin essential in dessication-rehydration process; expression induced by osmotic stress, starvation and during stationary phase; protein abundance increases in response to DNA replication stress Gene:YJR018W(YJR018W_d)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR154W(YJR154W_p)|FD-Score:3.83|P-value:6.35E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YKE2(YLR200W)|FD-Score:-4.75|P-value:1.02E-6||SGD DESC:Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin Gene:YKL169C(YKL169C_d)|FD-Score:4.78|P-value:8.98E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YLR046C(YLR046C_p)|FD-Score:-3.18|P-value:7.44E-4||SGD DESC:Putative membrane protein; member of the fungal lipid-translocating exporter (LTE) family of proteins; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance Gene:YLR111W(YLR111W_d)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR184W(YLR184W_d)|FD-Score:3.13|P-value:8.88E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML007C-A(YML007C-A_p)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YML020W(YML020W_p)|FD-Score:3.41|P-value:3.26E-4||SGD DESC:Putative protein of unknown function Gene:YMR103C(YMR103C_d)|FD-Score:-3.42|P-value:3.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR193C-A(YMR193C-A_d)|FD-Score:4.16|P-value:1.62E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR206W(YMR206W_p)|FD-Score:4.41|P-value:5.16E-6||SGD DESC:Putative protein of unknown function; not an essential gene; YMR206W has a paralog, YNR014W, that arose from the whole genome duplication Gene:YNL144C(YNL144C_p)|FD-Score:3.73|P-value:9.46E-5||SGD DESC:Putative protein of unknown function; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; contains a PH domain and binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study Gene:YNR068C(YNR068C_p)|FD-Score:7|P-value:1.30E-12||SGD DESC:Putative protein of unknown function; exhibits homology to C-terminal end of Bul1p; expressed as a readthrough product of BSC5, the readthrough locus being termed BUL3; the BUL3 readthrough product is involved in ubiquitin-mediated sorting of plasma membrane proteins and interacts with WW domains of Rsp5p in vitro, but in a functionally different way than the non-readthrough form Gene:YOR072W(YOR072W_d)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR082C(YOR082C_d)|FD-Score:-3.14|P-value:8.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene YOR083W Gene:YOR1(YGR281W)|FD-Score:3.89|P-value:5.07E-5||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR) Gene:YPL108W(YPL108W_p)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:Cytoplasmic protein of unknown function; non-essential gene that is induced in a GDH1 deleted strain with altered redox metabolism; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YPR012W(YPR012W_d)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR012W is not an essential gene Gene:YPR196W(YPR196W_p)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Putative maltose-responsive transcription factor Gene:YPT31(YER031C)|FD-Score:20.3|P-value:4.92E-92||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:YPT6(YLR262C)|FD-Score:-5.84|P-value:2.62E-9||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:AIM17(YHL021C)|FD-Score:-3.61|P-value:1.55E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:AKR1(YDR264C)|FD-Score:3.13|P-value:8.60E-4||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:ALE1(YOR175C)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Broad-specificity lysophospholipid acyltransferase, part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids Gene:APM1(YPL259C)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting Gene:APN1(YKL114C)|FD-Score:3.91|P-value:4.71E-5||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ARO1(YDR127W)|FD-Score:5.63|P-value:8.93E-9||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATG12(YBR217W)|FD-Score:5.81|P-value:3.15E-9||SGD DESC:Conserved ubiquitin-like modifier involved in autophagy and the Cvt pathway; conjugated to Atg5p to form a complex involved in Atg8p lipidation; Atg12p-Atg5p also forms a complex with Atg16p that is required for autophagosome formation Gene:ATP5(YDR298C)|FD-Score:-3.37|P-value:3.79E-4||SGD DESC:Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated Gene:BDH2(YAL061W_p)|FD-Score:-3.35|P-value:4.06E-4||SGD DESC:Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3 Gene:BNI4(YNL233W)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:BUB2(YMR055C)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Mitotic exit network regulator, forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage Gene:CBF1(YJR060W)|FD-Score:8.93|P-value:2.12E-19||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:CCM1(YGR150C)|FD-Score:-3.19|P-value:7.18E-4||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CDC50(YCR094W)|FD-Score:8.28|P-value:6.42E-17||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CHS5(YLR330W)|FD-Score:5.66|P-value:7.37E-9||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:CHS7(YHR142W)|FD-Score:4.65|P-value:1.65E-6||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:CLC1(YGR167W)|FD-Score:-6.96|P-value:1.68E-12||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COG7(YGL005C)|FD-Score:-6.13|P-value:4.36E-10||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ1(YBR003W)|FD-Score:3.11|P-value:9.46E-4||SGD DESC:Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis Gene:CPR7(YJR032W)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity Gene:CTF3(YLR381W)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Outer kinetochore protein that forms a complex with Mcm16p and Mcm22p; may bind the kinetochore to spindle microtubules; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-I and fission yeast mis6 Gene:CYS4(YGR155W)|FD-Score:7.41|P-value:6.48E-14||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DEG1(YFL001W)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability Gene:DFG16(YOR030W)|FD-Score:4.44|P-value:4.58E-6||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DUR1,2(YBR208C)|FD-Score:5.73|P-value:4.99E-9||SGD DESC:Urea amidolyase; contains both urea carboxylase and allophanate hydrolase activities, degrades urea to CO2 and NH3; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation; protein abundance increases in response to DNA replication stress Gene:ERG2(YMR202W)|FD-Score:-3.59|P-value:1.67E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERJ5(YFR041C)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:Type I membrane protein with a J domain is required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response Gene:FAA3(YIL009W)|FD-Score:3.74|P-value:9.21E-5||SGD DESC:Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:FCY1(YPR062W)|FD-Score:3.31|P-value:4.67E-4||SGD DESC:Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) Gene:FPR1(YNL135C)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function Gene:FRE6(YLL051C)|FD-Score:3.33|P-value:4.34E-4||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels Gene:GCG1(YER163C_p)|FD-Score:3.83|P-value:6.51E-5||SGD DESC:Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle Gene:GCN2(YDR283C)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCS1(YDL226C)|FD-Score:24.3|P-value:1.41E-130||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GYP1(YOR070C)|FD-Score:4.04|P-value:2.62E-5||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:IES6(YEL044W)|FD-Score:4.32|P-value:7.88E-6||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes Gene:IRS4(YKR019C)|FD-Score:-5.36|P-value:4.18E-8||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISW1(YBR245C)|FD-Score:4.49|P-value:3.49E-6||SGD DESC:ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p (Isw1a), and a complex with Ioc2p and Ioc4p (Isw1b); Isw1a and Isw1b have partially overlapping and distinct roles, Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation; Isw1b recruited to open reading frames by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions Gene:ISY1(YJR050W)|FD-Score:-3.5|P-value:2.34E-4||SGD DESC:Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles Gene:KCS1(YDR017C)|FD-Score:-3.49|P-value:2.44E-4||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response and telomere maintenance Gene:LCB4(YOR171C)|FD-Score:4.46|P-value:4.08E-6||SGD DESC:Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes Gene:MDY2(YOL111C)|FD-Score:11.7|P-value:5.73E-32||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MIG1(YGL035C)|FD-Score:3.33|P-value:4.34E-4||SGD DESC:Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion Gene:MKK2(YPL140C)|FD-Score:3.83|P-value:6.33E-5||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk1p; MKK2 has a paralog, MKK1, that arose from the whole genome duplication Gene:MMR1(YLR190W)|FD-Score:9.68|P-value:1.79E-22||SGD DESC:Phosphorylated protein of the mitochondrial outer membrane, localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p Gene:MNL1(YHR204W)|FD-Score:4.34|P-value:7.19E-6||SGD DESC:Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation Gene:MRI1(YPR118W)|FD-Score:3.19|P-value:7.14E-4||SGD DESC:5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway Gene:MSL1(YIR009W)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:U2B component of U2 snRNP, involved in splicing, binds the U2 snRNA stem-loop IV in vitro but requires association of Lea1p for in vivo binding; does not contain the conserved C-terminal RNA binding domain found in other family members Gene:MSN1(YOL116W)|FD-Score:4.4|P-value:5.39E-6||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:NOT3(YIL038C)|FD-Score:4.02|P-value:2.95E-5||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:NUT1(YGL151W)|FD-Score:-3.83|P-value:6.29E-5||SGD DESC:Component of the RNA polymerase II mediator complex, which is required for transcriptional activation and also has a role in basal transcription Gene:PAM1(YDR251W)|FD-Score:-3.26|P-value:5.51E-4||SGD DESC:Essential protein of unknown function; exhibits variable expression during colony morphogenesis; overexpression permits survival without protein phosphatase 2A, inhibits growth, and induces a filamentous phenotype Gene:PGC1(YPL206C)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Phosphatidyl Glycerol phospholipase C; regulates the phosphatidylglycerol (PG) content via a phospholipase C-type degradation mechanism; contains glycerophosphodiester phosphodiesterase motifs Gene:PIH1(YHR034C)|FD-Score:3.28|P-value:5.15E-4||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:PLB2(YMR006C)|FD-Score:-3.72|P-value:9.85E-5||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; displays transacylase activity in vitro; overproduction confers resistance to lysophosphatidylcholine Gene:PPT2(YPL148C)|FD-Score:-3.49|P-value:2.41E-4||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PUF3(YLL013C)|FD-Score:7.31|P-value:1.31E-13||SGD DESC:Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins Gene:RAD54(YGL163C)|FD-Score:3.23|P-value:6.13E-4||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:RAI1(YGL246C)|FD-Score:-5.03|P-value:2.45E-7||SGD DESC:Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z Gene:RCY1(YJL204C)|FD-Score:29.1|P-value:5.80E-187||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RGP1(YDR137W)|FD-Score:-8.46|P-value:1.33E-17||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIC1(YLR039C)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RIM8(YGL045W)|FD-Score:4.3|P-value:8.46E-6||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RIM9(YMR063W)|FD-Score:8.86|P-value:3.84E-19||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:ROY1(YMR258C)|FD-Score:4.31|P-value:8.14E-6||SGD DESC:GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p Gene:RPA34(YJL148W)|FD-Score:-3.22|P-value:6.37E-4||SGD DESC:RNA polymerase I subunit A34.5 Gene:RPL22A(YLR061W)|FD-Score:-5.75|P-value:4.36E-9||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPL35B(YDL136W)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RPS11A(YDR025W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication Gene:RPS27A(YKL156W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27A has a paralog, RPS27B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPS9B(YBR189W)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9B has a paralog, RPS9A, that arose from the whole genome duplication Gene:RRT6(YGL146C_p)|FD-Score:-4.06|P-value:2.48E-5||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; contains two putative transmembrane spans, but no significant homology to other known proteins Gene:SBA1(YKL117W)|FD-Score:-3.4|P-value:3.37E-4||SGD DESC:Co-chaperone that binds to and regulates Hsp90 family chaperones; important for pp60v-src activity in yeast; homologous to the mammalian p23 proteins, and like p23 can regulate telomerase activity; protein abundance increases in response to DNA replication stress Gene:SEC22(YLR268W)|FD-Score:3.2|P-value:6.94E-4||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SHE4(YOR035C)|FD-Score:4.36|P-value:6.37E-6||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SIA1(YOR137C)|FD-Score:4.62|P-value:1.95E-6||SGD DESC:Protein of unassigned function involved in activation of the Pma1p plasma membrane H+-ATPase by glucose Gene:SIN3(YOL004W)|FD-Score:4.76|P-value:9.62E-7||SGD DESC:Component of the Sin3p-Rpd3p histone deacetylase complex, involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity Gene:SLG1(YOR008C)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SNT309(YPR101W)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:STI1(YOR027W)|FD-Score:-3.09|P-value:9.85E-4||SGD DESC:Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop Gene:SVP26(YHR181W)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:Integral membrane protein of the early Golgi apparatus and endoplasmic reticulum, involved in COP II vesicle transport; may also function to promote retention of proteins in the early Golgi compartment Gene:SWA2(YDR320C)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:TDA3(YHR009C_p)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Putative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1 Gene:TOM1(YDR457W)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:TRM7(YBR061C)|FD-Score:-4.45|P-value:4.36E-6||SGD DESC:2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively Gene:TRP1(YDR007W)|FD-Score:3.94|P-value:4.08E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:7|P-value:1.25E-12||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:6.99|P-value:1.33E-12||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:21.4|P-value:9.22E-102||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TVP18(YMR071C)|FD-Score:6.15|P-value:3.83E-10||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:UBC12(YLR306W)|FD-Score:-3.41|P-value:3.21E-4||SGD DESC:Enzyme that mediates the conjugation of Rub1p, a ubiquitin-like protein, to other proteins; related to E2 ubiquitin-conjugating enzymes Gene:UBX6(YJL048C)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p, transcription is repressed when cells are grown in media containing inositol and choline; UBX6 has a paralog, UBX7, that arose from the whole genome duplication Gene:VPS20(YMR077C)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS61(YDR136C_d)|FD-Score:-6.17|P-value:3.33E-10||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:-4.54|P-value:2.76E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:VPS68(YOL129W)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria Gene:VPS71(YML041C)|FD-Score:-3.58|P-value:1.73E-4||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:VTA1(YLR181C)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Multivesicular body (MVB) protein involved in endosomal protein sorting; regulates Vps4p activity by promoting its oligomerization; has an N-terminal Vps60- and Did2- binding domain, a linker region, and a C-terminal Vps4p binding domain Gene:YCL001W-A(YCL001W-A_p)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YCR064C(YCR064C_d)|FD-Score:-5.38|P-value:3.68E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YDL011C(YDL011C_d)|FD-Score:-3.2|P-value:6.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YDL010W Gene:YDR008C(YDR008C_d)|FD-Score:14.2|P-value:6.53E-46||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR179W-A(YDR179W-A_p)|FD-Score:4.32|P-value:7.93E-6||SGD DESC:Putative protein of unknown function Gene:YDR282C(YDR282C_p)|FD-Score:3.86|P-value:5.78E-5||SGD DESC:Putative protein of unknown function; localizes to the inner mitochondrial membrane with the C terminus facing the intermembrane space; ortholog of human RMND1, mutation in which is implicated in infantile encephaloneuromyopathy and defective mitochondrial translation Gene:YEL1(YBL060W)|FD-Score:3.87|P-value:5.53E-5||SGD DESC:Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip Gene:YER084W(YER084W_p)|FD-Score:5.7|P-value:6.13E-9||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YFL040W(YFL040W_p)|FD-Score:-3.1|P-value:9.52E-4||SGD DESC:Putative transporter, member of the sugar porter family; YFL040W is not an essential gene Gene:YGL082W(YGL082W_p)|FD-Score:-3.24|P-value:5.89E-4||SGD DESC:Putative protein of unknown function; predicted prenylation/proteolysis target of Afc1p and Rce1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YGL082W is not an essential gene Gene:YGL159W(YGL159W_p)|FD-Score:4.64|P-value:1.77E-6||SGD DESC:Putative protein of unknown function; deletion mutant has no detectable phenotype Gene:YGR122W(YGR122W)|FD-Score:6.09|P-value:5.69E-10||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YHL037C(YHL037C_d)|FD-Score:3.77|P-value:8.01E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL001W(YIL001W_p)|FD-Score:-3.48|P-value:2.55E-4||SGD DESC:Putative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm Gene:YJL119C(YJL119C_d)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL144W(YJL144W)|FD-Score:-3.44|P-value:2.87E-4||SGD DESC:Cytoplasmic hydrophilin essential in dessication-rehydration process; expression induced by osmotic stress, starvation and during stationary phase; protein abundance increases in response to DNA replication stress Gene:YJR018W(YJR018W_d)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR154W(YJR154W_p)|FD-Score:3.83|P-value:6.35E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YKE2(YLR200W)|FD-Score:-4.75|P-value:1.02E-6||SGD DESC:Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin Gene:YKL169C(YKL169C_d)|FD-Score:4.78|P-value:8.98E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YLR046C(YLR046C_p)|FD-Score:-3.18|P-value:7.44E-4||SGD DESC:Putative membrane protein; member of the fungal lipid-translocating exporter (LTE) family of proteins; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance Gene:YLR111W(YLR111W_d)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR184W(YLR184W_d)|FD-Score:3.13|P-value:8.88E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML007C-A(YML007C-A_p)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YML020W(YML020W_p)|FD-Score:3.41|P-value:3.26E-4||SGD DESC:Putative protein of unknown function Gene:YMR103C(YMR103C_d)|FD-Score:-3.42|P-value:3.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR193C-A(YMR193C-A_d)|FD-Score:4.16|P-value:1.62E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR206W(YMR206W_p)|FD-Score:4.41|P-value:5.16E-6||SGD DESC:Putative protein of unknown function; not an essential gene; YMR206W has a paralog, YNR014W, that arose from the whole genome duplication Gene:YNL144C(YNL144C_p)|FD-Score:3.73|P-value:9.46E-5||SGD DESC:Putative protein of unknown function; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; contains a PH domain and binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study Gene:YNR068C(YNR068C_p)|FD-Score:7|P-value:1.30E-12||SGD DESC:Putative protein of unknown function; exhibits homology to C-terminal end of Bul1p; expressed as a readthrough product of BSC5, the readthrough locus being termed BUL3; the BUL3 readthrough product is involved in ubiquitin-mediated sorting of plasma membrane proteins and interacts with WW domains of Rsp5p in vitro, but in a functionally different way than the non-readthrough form Gene:YOR072W(YOR072W_d)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR082C(YOR082C_d)|FD-Score:-3.14|P-value:8.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene YOR083W Gene:YOR1(YGR281W)|FD-Score:3.89|P-value:5.07E-5||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR) Gene:YPL108W(YPL108W_p)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:Cytoplasmic protein of unknown function; non-essential gene that is induced in a GDH1 deleted strain with altered redox metabolism; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YPR012W(YPR012W_d)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR012W is not an essential gene Gene:YPR196W(YPR196W_p)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Putative maltose-responsive transcription factor Gene:YPT31(YER031C)|FD-Score:20.3|P-value:4.92E-92||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:YPT6(YLR262C)|FD-Score:-5.84|P-value:2.62E-9||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL055W19.607.02E-866.79OLE1Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
YOL144W12.807.07E-382.24NOP8Nucleolar protein required for 60S ribosomal subunit biogenesis
YJL195C_d10.601.94E-263.32YJL195C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6
YPR113W7.261.95E-130.09PIS1Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins
YOL120C7.173.74E-130.25RPL18ARibosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication
YLR009W6.922.26E-120.21RLP24Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
YDL196W_d6.719.43E-120.02YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YGL116W6.701.07E-110.15CDC20Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
YDL143W6.543.02E-110.39CCT4Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YBL105C6.163.70E-100.05PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YDR212W6.105.19E-100.00TCP1Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein
YOR206W6.105.24E-100.01NOC2Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors
YJR007W6.105.45E-100.07SUI2Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress
YMR203W6.038.39E-100.06TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YGL099W5.971.18E-90.07LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJL204C29.105.80E-187RCY1F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
YDL226C24.301.41E-130GCS1ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p
YDR354W21.409.22E-102TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YER031C20.304.92E-92YPT31Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication
YDR008C_d14.206.53E-46YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YOL111C11.705.73E-32MDY2Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes
YLR190W9.681.79E-22MMR1Phosphorylated protein of the mitochondrial outer membrane, localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p
YJR060W8.932.12E-19CBF1Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress
YMR063W8.863.84E-19RIM9Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI;
YCR094W8.286.42E-17CDC50Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication
YGR155W7.416.48E-14CYS4Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria
YLL013C7.311.31E-13PUF3Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins
YER090W7.001.25E-12TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YNR068C_p7.001.30E-12YNR068C_pPutative protein of unknown function; exhibits homology to C-terminal end of Bul1p; expressed as a readthrough product of BSC5, the readthrough locus being termed BUL3; the BUL3 readthrough product is involved in ubiquitin-mediated sorting of plasma membrane proteins and interacts with WW domains of Rsp5p in vitro, but in a functionally different way than the non-readthrough form
YKL211C6.991.33E-12TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p

GO enrichment analysis for SGTC_2993
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.5120SGTC_30819117243 49.5 μMChembridge (Drug-like library)455955890.136364fatty acid desaturase (OLE1)
0.4980SGTC_2725haloperidol 53.2 μMMiscellaneous35590.246154plasma membrane duress
0.4630SGTC_31269127922 49.5 μMChembridge (Drug-like library)455956820.130435fatty acid desaturase (OLE1)
0.4620SGTC_20505263056 171.0 μMChembridge (Fragment library)7634680.375fatty acid desaturase (OLE1)
0.4573.08E-302SGTC_258haloperidol 50.8 μMMiscellaneous35590.246154fatty acid desaturase (OLE1)
0.4459.21E-285SGTC_2733amorolfine 100.0 μMMiscellaneous542600.134328plasma membrane duress
0.4344.31E-270SGTC_2784092-0821 23.9 μMChemDiv (Drug-like library)8779000.47619plasma membrane duress
0.4172.63E-247SGTC_28809042983 58.4 μMChembridge (Drug-like library)64647140.568627fatty acid desaturase (OLE1)
0.4163.94E-246SGTC_1999st074831 12.5 μMTimTec (Natural product derivative library)171125230.35plasma membrane duress
0.4108.57E-239SGTC_20384100038 31.0 μMChembridge (Fragment library)35968030.137931plasma membrane duress
0.4083.12E-235SGTC_2721ifenprodil 61.4 μMMiscellaneous36890.169231plasma membrane duress
0.4081.10E-235SGTC_10054092-0690 4.4 μMChemDiv (Drug-like library)15293430.222222plasma membrane duress
0.4081.47E-235SGTC_30719117417 49.5 μMChembridge (Drug-like library)455955900.142857fatty acid desaturase (OLE1)
0.4072.52E-234SGTC_28389002687 71.4 μMChembridge (Drug-like library)64570600.632653fatty acid desaturase (OLE1)
0.4032.70E-229SGTC_28879044784 58.4 μMChembridge (Drug-like library)64655460.761905fatty acid desaturase (OLE1)

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2887904478458.44 μM0.7619056465546Chembridge (Drug-like library)323.4322.98313fatty acid desaturase (OLE1)
SGTC_2895905444758.44 μM0.6444442697450Chembridge (Drug-like library)343.850483.16113
SGTC_2838900268771.43 μM0.6326536457060Chembridge (Drug-like library)357.877063.64713fatty acid desaturase (OLE1)
SGTC_2880904298358.44 μM0.5686276464714Chembridge (Drug-like library)341.4224633.18914fatty acid desaturase (OLE1)
SGTC_2835900658871.43 μM0.56252345538Chembridge (Drug-like library)300.782683.27912
SGTC_2893904848825.97 μM0.551026467338Chembridge (Drug-like library)337.458583.46913fatty acid desaturase (OLE1)
SGTC_3033909088749.47 μM0.54166719325384Chembridge (Drug-like library)342.862423.9402fatty acid desaturase (OLE1)
SGTC_2792753523581.82 μM0.5283021281891Chembridge (Drug-like library)376.900243.43314fatty acid desaturase (OLE1)
SGTC_2896905265671.43 μM0.5185196469436Chembridge (Drug-like library)369.887763.62913endomembrane recycling
SGTC_5154092-1064102 μM0.477273717353ChemDiv (Drug-like library)335.27084.77302plasma membrane duress