9053520

N-(2-chlorophenyl)-2-(3,4-dihydro-1H-isoquinolin-2-yl)acetamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2994
Screen concentration 71.4 μM
Source Chembridge (Drug-like library)
PubChem CID 4647384
SMILES C1CN(CC2=CC=CC=C21)CC(=O)NC3=CC=CC=C3Cl
Standardized SMILES Clc1ccccc1NC(=O)CN2CCc3ccccc3C2
Molecular weight 300.7827
ALogP 3.28
H-bond donor count 1
H-bond acceptor count 2
Response signature RPP1 & pyrimidine depletion

Pool Growth Kinetics
% growth inhibition (Het. pool) 10.37
% growth inhibition (Hom. pool) 8.24


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4647384
Download HIP data (tab-delimited text)  (excel)
Gene:ARB1(YER036C)|FD-Score:3.21|P-value:6.63E-4|Clearance:0.2||SGD DESC:ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p Gene:CCA1(YER168C)|FD-Score:6.34|P-value:1.14E-10|Clearance:1.35||SGD DESC:ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites Gene:CDC20(YGL116W)|FD-Score:3.74|P-value:9.17E-5|Clearance:0.16||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CLP1(YOR250C)|FD-Score:3.96|P-value:3.80E-5|Clearance:0.19||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:DED1(YOR204W)|FD-Score:4|P-value:3.23E-5|Clearance:0.04||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:ESP1(YGR098C)|FD-Score:4.77|P-value:9.18E-7|Clearance:0.32||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:ESS1(YJR017C)|FD-Score:3.32|P-value:4.44E-4|Clearance:0.11||SGD DESC:Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated serine and threonine residues N-terminal to proline; regulates phosphorylation of the RNAP II large subunit (Rpo21p) C-terminal domain (CTD); associates with phospho-Ser5 form of RNAP II in vivo; regulates phosphorylation of Ser7 within CTD; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription and trimethylation of histone H3 Gene:NIC96(YFR002W)|FD-Score:-3.6|P-value:1.60E-4|Clearance:0||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:NSA2(YER126C)|FD-Score:3.77|P-value:8.33E-5|Clearance:0.02||SGD DESC:Protein constituent of 66S pre-ribosomal particles, contributes to processing of the 27S pre-rRNA Gene:PDI1(YCL043C)|FD-Score:6.28|P-value:1.71E-10|Clearance:1.35||SGD DESC:Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication Gene:PWP2(YCR057C)|FD-Score:-6.13|P-value:4.33E-10|Clearance:0||SGD DESC:Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis Gene:RPP1(YHR062C)|FD-Score:4.92|P-value:4.24E-7|Clearance:0.15||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SAM35(YHR083W)|FD-Score:3.56|P-value:1.84E-4|Clearance:0.06||SGD DESC:Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane Gene:SPT5(YML010W)|FD-Score:4.2|P-value:1.35E-5|Clearance:0.2||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair Gene:STU1(YBL034C)|FD-Score:3.5|P-value:2.35E-4|Clearance:0.17||SGD DESC:Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles Gene:STU2(YLR045C)|FD-Score:4.45|P-value:4.36E-6|Clearance:0.25||SGD DESC:Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics during spindle orientation and metaphase chromosome alignment; interacts with spindle pole body component Spc72p Gene:YJL195C(YJL195C_d)|FD-Score:7.02|P-value:1.08E-12|Clearance:1.35||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YJR141W(YJR141W_p)|FD-Score:3.58|P-value:1.69E-4|Clearance:0.02||SGD DESC:Essential protein of unknown function Gene:ARB1(YER036C)|FD-Score:3.21|P-value:6.63E-4|Clearance:0.2||SGD DESC:ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p Gene:CCA1(YER168C)|FD-Score:6.34|P-value:1.14E-10|Clearance:1.35||SGD DESC:ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites Gene:CDC20(YGL116W)|FD-Score:3.74|P-value:9.17E-5|Clearance:0.16||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CLP1(YOR250C)|FD-Score:3.96|P-value:3.80E-5|Clearance:0.19||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:DED1(YOR204W)|FD-Score:4|P-value:3.23E-5|Clearance:0.04||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:ESP1(YGR098C)|FD-Score:4.77|P-value:9.18E-7|Clearance:0.32||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:ESS1(YJR017C)|FD-Score:3.32|P-value:4.44E-4|Clearance:0.11||SGD DESC:Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated serine and threonine residues N-terminal to proline; regulates phosphorylation of the RNAP II large subunit (Rpo21p) C-terminal domain (CTD); associates with phospho-Ser5 form of RNAP II in vivo; regulates phosphorylation of Ser7 within CTD; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription and trimethylation of histone H3 Gene:NIC96(YFR002W)|FD-Score:-3.6|P-value:1.60E-4|Clearance:0||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:NSA2(YER126C)|FD-Score:3.77|P-value:8.33E-5|Clearance:0.02||SGD DESC:Protein constituent of 66S pre-ribosomal particles, contributes to processing of the 27S pre-rRNA Gene:PDI1(YCL043C)|FD-Score:6.28|P-value:1.71E-10|Clearance:1.35||SGD DESC:Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication Gene:PWP2(YCR057C)|FD-Score:-6.13|P-value:4.33E-10|Clearance:0||SGD DESC:Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis Gene:RPP1(YHR062C)|FD-Score:4.92|P-value:4.24E-7|Clearance:0.15||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SAM35(YHR083W)|FD-Score:3.56|P-value:1.84E-4|Clearance:0.06||SGD DESC:Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane Gene:SPT5(YML010W)|FD-Score:4.2|P-value:1.35E-5|Clearance:0.2||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair Gene:STU1(YBL034C)|FD-Score:3.5|P-value:2.35E-4|Clearance:0.17||SGD DESC:Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles Gene:STU2(YLR045C)|FD-Score:4.45|P-value:4.36E-6|Clearance:0.25||SGD DESC:Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics during spindle orientation and metaphase chromosome alignment; interacts with spindle pole body component Spc72p Gene:YJL195C(YJL195C_d)|FD-Score:7.02|P-value:1.08E-12|Clearance:1.35||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YJR141W(YJR141W_p)|FD-Score:3.58|P-value:1.69E-4|Clearance:0.02||SGD DESC:Essential protein of unknown function

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4647384
Download HOP data (tab-delimited text)  (excel)
Gene:AGP1(YCL025C)|FD-Score:6.09|P-value:5.73E-10||SGD DESC:Low-affinity amino acid permease with broad substrate range; involved in uptake of asparagine, glutamine, and other amino acids; expression regulated by SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); AGP1 has a paralog, GNP1, that arose from the whole genome duplication Gene:AIM17(YHL021C)|FD-Score:-4.08|P-value:2.24E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:AIM26(YKL037W)|FD-Score:3.94|P-value:4.02E-5||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:AIM3(YBR108W)|FD-Score:-3.51|P-value:2.24E-4||SGD DESC:Protein interacting with Rvs167p; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ALG6(YOR002W)|FD-Score:4.71|P-value:1.23E-6||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:ANP1(YEL036C)|FD-Score:4.97|P-value:3.42E-7||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:ATG8(YBL078C)|FD-Score:4.25|P-value:1.09E-5||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:ATO3(YDR384C)|FD-Score:-3.17|P-value:7.69E-4||SGD DESC:Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters Gene:BTS1(YPL069C)|FD-Score:-4.43|P-value:4.80E-6||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CAC2(YML102W)|FD-Score:-3.35|P-value:4.05E-4||SGD DESC:Subunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1p; chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:CBS2(YDR197W)|FD-Score:-3.14|P-value:8.48E-4||SGD DESC:Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader Gene:CTK3(YML112W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:CWH41(YGL027C)|FD-Score:-3.14|P-value:8.40E-4||SGD DESC:Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress Gene:DDI3(YNL335W_p)|FD-Score:-5.55|P-value:1.44E-8||SGD DESC:Protein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants Gene:EMP70(YLR083C)|FD-Score:-3.69|P-value:1.12E-4||SGD DESC:Protein with a role in cellular adhesion, filamentous growth, and endosome-to-vacuole sorting; similar to Tmn2p and Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments Gene:ERP3(YDL018C)|FD-Score:-3.37|P-value:3.75E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:ERV25(YML012W)|FD-Score:4.02|P-value:2.93E-5||SGD DESC:Protein that forms a heterotrimeric complex with Erp1, Erp2p, and Emp24, member of the p24 family involved in endoplasmic reticulum to Golgi transport Gene:FCY1(YPR062W)|FD-Score:4.99|P-value:2.96E-7||SGD DESC:Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) Gene:FLO11(YIR019C)|FD-Score:6.63|P-value:1.65E-11||SGD DESC:GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p); required for the formation of fibrous interconnections between cells in a colony of a wild S. cerevisiae strain Gene:FRE5(YOR384W)|FD-Score:5.07|P-value:1.97E-7||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GCG1(YER163C_p)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle Gene:GFD1(YMR255W)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:Coiled-coiled protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; protein abundance increases in response to DNA replication stress Gene:GSF2(YML048W)|FD-Score:4.96|P-value:3.58E-7||SGD DESC:ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression Gene:HBT1(YDL223C)|FD-Score:3.72|P-value:9.83E-5||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:IDS2(YJL146W)|FD-Score:-4.83|P-value:6.73E-7||SGD DESC:Protein involved in modulation of Ime2p activity during meiosis, appears to act indirectly to promote Ime2p-mediated late meiotic functions; found in growing cells and degraded during sporulation Gene:ISC10(YER180C)|FD-Score:-3.44|P-value:2.87E-4||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:KAR5(YMR065W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Protein required for nuclear membrane fusion during karyogamy, localizes to the membrane with a soluble portion in the endoplasmic reticulum lumen, may form a complex with Jem1p and Kar2p; expression of the gene is regulated by pheromone Gene:KTR7(YIL085C)|FD-Score:4.79|P-value:8.49E-7||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication Gene:LCB4(YOR171C)|FD-Score:7.29|P-value:1.50E-13||SGD DESC:Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes Gene:LCL3(YGL085W_p)|FD-Score:3.23|P-value:6.29E-4||SGD DESC:Putative protein of unknown function; mutant has long chronological lifespan; has homology to Staphylococcus aureus nuclease; GFP-fusion protein localizes to mitochondria; is induced in response to the DNA-damaging agent MMS Gene:MAK10(YEL053C)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Non-catalytic subunit of N-terminal acetyltransferase of the NatC type, required for replication of dsRNA virus; expression is glucose-repressible Gene:MAL13(YGR288W)|FD-Score:3.09|P-value:9.93E-4||SGD DESC:MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C Gene:MAL31(YBR298C)|FD-Score:3.86|P-value:5.70E-5||SGD DESC:Maltose permease, high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL3 complex locus; member of the 12 transmembrane domain superfamily of sugar transporters; functional in genomic reference strain S288C Gene:MCT1(YOR221C)|FD-Score:4|P-value:3.20E-5||SGD DESC:Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling Gene:MLH1(YMR167W)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:Protein required for mismatch repair in mitosis and meiosis as well as crossing over during meiosis; forms a complex with Pms1p and Msh2p-Msh3p during mismatch repair; human homolog is associated with hereditary non-polyposis colon cancer Gene:MMR1(YLR190W)|FD-Score:4.15|P-value:1.64E-5||SGD DESC:Phosphorylated protein of the mitochondrial outer membrane, localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p Gene:MPM1(YJL066C)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Mitochondrial intermembrane space protein of unknown function Gene:MSW1(YDR268W)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:OPT2(YPR194C)|FD-Score:3.95|P-value:3.89E-5||SGD DESC:Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles Gene:PES4(YFR023W)|FD-Score:-3.78|P-value:7.76E-5||SGD DESC:Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication Gene:PPT2(YPL148C)|FD-Score:-4.13|P-value:1.84E-5||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PRY2(YKR013W)|FD-Score:-3.21|P-value:6.68E-4||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication Gene:PYK2(YOR347C)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication Gene:RAV1(YJR033C)|FD-Score:7.41|P-value:6.11E-14||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RPL29(YFR032C-A)|FD-Score:3.1|P-value:9.54E-4||SGD DESC:Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog Gene:RPL35B(YDL136W)|FD-Score:4.27|P-value:9.62E-6||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RPS21B(YJL136C)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication Gene:RPS22A(YJL190C)|FD-Score:3.76|P-value:8.54E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication Gene:RPS9B(YBR189W)|FD-Score:4.8|P-value:7.85E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9B has a paralog, RPS9A, that arose from the whole genome duplication Gene:SGA1(YIL099W)|FD-Score:-3.26|P-value:5.65E-4||SGD DESC:Intracellular sporulation-specific glucoamylase involved in glycogen degradation; induced during starvation of a/a diploids late in sporulation, but dispensable for sporulation Gene:SPG5(YMR191W)|FD-Score:-4.29|P-value:9.04E-6||SGD DESC:Protein required for proteasome assembly during quiescence; binds to base of the proteasome regulartory particle; required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources Gene:SPO11(YHL022C)|FD-Score:6.32|P-value:1.28E-10||SGD DESC:Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation Gene:SRN2(YLR119W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus Gene:SSH1(YBR283C)|FD-Score:-3.31|P-value:4.67E-4||SGD DESC:Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential Gene:SSO2(YMR183C)|FD-Score:-3.37|P-value:3.72E-4||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p Gene:TED1(YIL039W)|FD-Score:3.98|P-value:3.47E-5||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:TGS1(YPL157W)|FD-Score:-4.03|P-value:2.78E-5||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TPP1(YMR156C)|FD-Score:-3.16|P-value:7.91E-4||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:UBP12(YJL197W)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; present in the nucleus and cytoplasm Gene:VAN1(YML115C)|FD-Score:3.25|P-value:5.77E-4||SGD DESC:Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant Gene:VID24(YBR105C)|FD-Score:-3.22|P-value:6.52E-4||SGD DESC:GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles Gene:VPS63(YLR261C_d)|FD-Score:4.34|P-value:7.04E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YBR139W(YBR139W)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Putative serine type carboxypeptidase with a role in phytochelatin synthesis; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner Gene:YBR226C(YBR226C_d)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YCL012C(YCL012C)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene Gene:YHR049C-A(YHR049C-A_d)|FD-Score:4.59|P-value:2.20E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR038C(YJR038C_d)|FD-Score:5.15|P-value:1.27E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLL007C(YLL007C_p)|FD-Score:-3.74|P-value:9.27E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YLL059C(YLL059C_d)|FD-Score:4.88|P-value:5.30E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR040C(YLR040C_p)|FD-Score:-4.49|P-value:3.50E-6||SGD DESC:Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential Gene:YLR042C(YLR042C_p)|FD-Score:5.17|P-value:1.16E-7||SGD DESC:Protein of unknown function; localizes to the cytoplasm; YLL042C is not an essential gene Gene:YML018C(YML018C_p)|FD-Score:-3.33|P-value:4.32E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication Gene:YML122C(YML122C_d)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR262W(YMR262W_p)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene Gene:YPL041C(YPL041C_p)|FD-Score:-3.28|P-value:5.24E-4||SGD DESC:Protein of unknown function involved in maintenance of proper telomere length Gene:AGP1(YCL025C)|FD-Score:6.09|P-value:5.73E-10||SGD DESC:Low-affinity amino acid permease with broad substrate range; involved in uptake of asparagine, glutamine, and other amino acids; expression regulated by SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); AGP1 has a paralog, GNP1, that arose from the whole genome duplication Gene:AIM17(YHL021C)|FD-Score:-4.08|P-value:2.24E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:AIM26(YKL037W)|FD-Score:3.94|P-value:4.02E-5||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:AIM3(YBR108W)|FD-Score:-3.51|P-value:2.24E-4||SGD DESC:Protein interacting with Rvs167p; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ALG6(YOR002W)|FD-Score:4.71|P-value:1.23E-6||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:ANP1(YEL036C)|FD-Score:4.97|P-value:3.42E-7||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:ATG8(YBL078C)|FD-Score:4.25|P-value:1.09E-5||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:ATO3(YDR384C)|FD-Score:-3.17|P-value:7.69E-4||SGD DESC:Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters Gene:BTS1(YPL069C)|FD-Score:-4.43|P-value:4.80E-6||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CAC2(YML102W)|FD-Score:-3.35|P-value:4.05E-4||SGD DESC:Subunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1p; chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:CBS2(YDR197W)|FD-Score:-3.14|P-value:8.48E-4||SGD DESC:Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader Gene:CTK3(YML112W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:CWH41(YGL027C)|FD-Score:-3.14|P-value:8.40E-4||SGD DESC:Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress Gene:DDI3(YNL335W_p)|FD-Score:-5.55|P-value:1.44E-8||SGD DESC:Protein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants Gene:EMP70(YLR083C)|FD-Score:-3.69|P-value:1.12E-4||SGD DESC:Protein with a role in cellular adhesion, filamentous growth, and endosome-to-vacuole sorting; similar to Tmn2p and Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments Gene:ERP3(YDL018C)|FD-Score:-3.37|P-value:3.75E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:ERV25(YML012W)|FD-Score:4.02|P-value:2.93E-5||SGD DESC:Protein that forms a heterotrimeric complex with Erp1, Erp2p, and Emp24, member of the p24 family involved in endoplasmic reticulum to Golgi transport Gene:FCY1(YPR062W)|FD-Score:4.99|P-value:2.96E-7||SGD DESC:Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) Gene:FLO11(YIR019C)|FD-Score:6.63|P-value:1.65E-11||SGD DESC:GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p); required for the formation of fibrous interconnections between cells in a colony of a wild S. cerevisiae strain Gene:FRE5(YOR384W)|FD-Score:5.07|P-value:1.97E-7||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GCG1(YER163C_p)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle Gene:GFD1(YMR255W)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:Coiled-coiled protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; protein abundance increases in response to DNA replication stress Gene:GSF2(YML048W)|FD-Score:4.96|P-value:3.58E-7||SGD DESC:ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression Gene:HBT1(YDL223C)|FD-Score:3.72|P-value:9.83E-5||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:IDS2(YJL146W)|FD-Score:-4.83|P-value:6.73E-7||SGD DESC:Protein involved in modulation of Ime2p activity during meiosis, appears to act indirectly to promote Ime2p-mediated late meiotic functions; found in growing cells and degraded during sporulation Gene:ISC10(YER180C)|FD-Score:-3.44|P-value:2.87E-4||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:KAR5(YMR065W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Protein required for nuclear membrane fusion during karyogamy, localizes to the membrane with a soluble portion in the endoplasmic reticulum lumen, may form a complex with Jem1p and Kar2p; expression of the gene is regulated by pheromone Gene:KTR7(YIL085C)|FD-Score:4.79|P-value:8.49E-7||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication Gene:LCB4(YOR171C)|FD-Score:7.29|P-value:1.50E-13||SGD DESC:Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes Gene:LCL3(YGL085W_p)|FD-Score:3.23|P-value:6.29E-4||SGD DESC:Putative protein of unknown function; mutant has long chronological lifespan; has homology to Staphylococcus aureus nuclease; GFP-fusion protein localizes to mitochondria; is induced in response to the DNA-damaging agent MMS Gene:MAK10(YEL053C)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Non-catalytic subunit of N-terminal acetyltransferase of the NatC type, required for replication of dsRNA virus; expression is glucose-repressible Gene:MAL13(YGR288W)|FD-Score:3.09|P-value:9.93E-4||SGD DESC:MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C Gene:MAL31(YBR298C)|FD-Score:3.86|P-value:5.70E-5||SGD DESC:Maltose permease, high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL3 complex locus; member of the 12 transmembrane domain superfamily of sugar transporters; functional in genomic reference strain S288C Gene:MCT1(YOR221C)|FD-Score:4|P-value:3.20E-5||SGD DESC:Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling Gene:MLH1(YMR167W)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:Protein required for mismatch repair in mitosis and meiosis as well as crossing over during meiosis; forms a complex with Pms1p and Msh2p-Msh3p during mismatch repair; human homolog is associated with hereditary non-polyposis colon cancer Gene:MMR1(YLR190W)|FD-Score:4.15|P-value:1.64E-5||SGD DESC:Phosphorylated protein of the mitochondrial outer membrane, localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p Gene:MPM1(YJL066C)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Mitochondrial intermembrane space protein of unknown function Gene:MSW1(YDR268W)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:OPT2(YPR194C)|FD-Score:3.95|P-value:3.89E-5||SGD DESC:Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles Gene:PES4(YFR023W)|FD-Score:-3.78|P-value:7.76E-5||SGD DESC:Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication Gene:PPT2(YPL148C)|FD-Score:-4.13|P-value:1.84E-5||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PRY2(YKR013W)|FD-Score:-3.21|P-value:6.68E-4||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication Gene:PYK2(YOR347C)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication Gene:RAV1(YJR033C)|FD-Score:7.41|P-value:6.11E-14||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RPL29(YFR032C-A)|FD-Score:3.1|P-value:9.54E-4||SGD DESC:Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog Gene:RPL35B(YDL136W)|FD-Score:4.27|P-value:9.62E-6||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RPS21B(YJL136C)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication Gene:RPS22A(YJL190C)|FD-Score:3.76|P-value:8.54E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication Gene:RPS9B(YBR189W)|FD-Score:4.8|P-value:7.85E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9B has a paralog, RPS9A, that arose from the whole genome duplication Gene:SGA1(YIL099W)|FD-Score:-3.26|P-value:5.65E-4||SGD DESC:Intracellular sporulation-specific glucoamylase involved in glycogen degradation; induced during starvation of a/a diploids late in sporulation, but dispensable for sporulation Gene:SPG5(YMR191W)|FD-Score:-4.29|P-value:9.04E-6||SGD DESC:Protein required for proteasome assembly during quiescence; binds to base of the proteasome regulartory particle; required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources Gene:SPO11(YHL022C)|FD-Score:6.32|P-value:1.28E-10||SGD DESC:Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation Gene:SRN2(YLR119W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus Gene:SSH1(YBR283C)|FD-Score:-3.31|P-value:4.67E-4||SGD DESC:Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential Gene:SSO2(YMR183C)|FD-Score:-3.37|P-value:3.72E-4||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p Gene:TED1(YIL039W)|FD-Score:3.98|P-value:3.47E-5||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:TGS1(YPL157W)|FD-Score:-4.03|P-value:2.78E-5||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TPP1(YMR156C)|FD-Score:-3.16|P-value:7.91E-4||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:UBP12(YJL197W)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; present in the nucleus and cytoplasm Gene:VAN1(YML115C)|FD-Score:3.25|P-value:5.77E-4||SGD DESC:Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant Gene:VID24(YBR105C)|FD-Score:-3.22|P-value:6.52E-4||SGD DESC:GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles Gene:VPS63(YLR261C_d)|FD-Score:4.34|P-value:7.04E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YBR139W(YBR139W)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Putative serine type carboxypeptidase with a role in phytochelatin synthesis; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner Gene:YBR226C(YBR226C_d)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YCL012C(YCL012C)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene Gene:YHR049C-A(YHR049C-A_d)|FD-Score:4.59|P-value:2.20E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR038C(YJR038C_d)|FD-Score:5.15|P-value:1.27E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLL007C(YLL007C_p)|FD-Score:-3.74|P-value:9.27E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YLL059C(YLL059C_d)|FD-Score:4.88|P-value:5.30E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR040C(YLR040C_p)|FD-Score:-4.49|P-value:3.50E-6||SGD DESC:Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential Gene:YLR042C(YLR042C_p)|FD-Score:5.17|P-value:1.16E-7||SGD DESC:Protein of unknown function; localizes to the cytoplasm; YLL042C is not an essential gene Gene:YML018C(YML018C_p)|FD-Score:-3.33|P-value:4.32E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication Gene:YML122C(YML122C_d)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR262W(YMR262W_p)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene Gene:YPL041C(YPL041C_p)|FD-Score:-3.28|P-value:5.24E-4||SGD DESC:Protein of unknown function involved in maintenance of proper telomere length

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJL195C_d7.021.08E-121.35YJL195C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6
YER168C6.341.14E-101.35CCA1ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites
YCL043C6.281.71E-101.35PDI1Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication
YHR062C4.924.24E-70.15RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YGR098C4.779.18E-70.32ESP1Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress
YLR045C4.454.36E-60.25STU2Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics during spindle orientation and metaphase chromosome alignment; interacts with spindle pole body component Spc72p
YML010W4.201.35E-50.20SPT5Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair
YOR204W4.003.23E-50.04DED1ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility
YOR250C3.963.80E-50.19CLP1Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation
YER126C3.778.33E-50.02NSA2Protein constituent of 66S pre-ribosomal particles, contributes to processing of the 27S pre-rRNA
YGL116W3.749.17E-50.16CDC20Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
YJR141W_p3.581.69E-40.02YJR141W_pEssential protein of unknown function
YHR083W3.561.84E-40.06SAM35Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane
YBL034C3.502.35E-40.17STU1Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles
YJR017C3.324.44E-40.11ESS1Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated serine and threonine residues N-terminal to proline; regulates phosphorylation of the RNAP II large subunit (Rpo21p) C-terminal domain (CTD); associates with phospho-Ser5 form of RNAP II in vivo; regulates phosphorylation of Ser7 within CTD; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription and trimethylation of histone H3

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJR033C7.416.11E-14RAV1Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate
YOR171C7.291.50E-13LCB4Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes
YIR019C6.631.65E-11FLO11GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p); required for the formation of fibrous interconnections between cells in a colony of a wild S. cerevisiae strain
YHL022C6.321.28E-10SPO11Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation
YCL025C6.095.73E-10AGP1Low-affinity amino acid permease with broad substrate range; involved in uptake of asparagine, glutamine, and other amino acids; expression regulated by SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); AGP1 has a paralog, GNP1, that arose from the whole genome duplication
YLR042C_p5.171.16E-7YLR042C_pProtein of unknown function; localizes to the cytoplasm; YLL042C is not an essential gene
YJR038C_d5.151.27E-7YJR038C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YOR384W5.071.97E-7FRE5Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YPR062W4.992.96E-7FCY1Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU)
YEL036C4.973.42E-7ANP1Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol
YML048W4.963.58E-7GSF2ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression
YLL059C_d4.885.30E-7YLL059C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YBR189W4.807.85E-7RPS9BProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9B has a paralog, RPS9A, that arose from the whole genome duplication
YIL085C4.798.49E-7KTR7Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication
YOR002W4.711.23E-6ALG6Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease

GO enrichment analysis for SGTC_2994
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0895.81E-12SGTC_265mycophenolic acid 32.9 μMMiscellaneous4465410.075RPP1 & pyrimidine depletion
0.0766.24E-9SGTC_256clotrimazole 625.0 nMMiscellaneous28120.147541ergosterol depletion effects on membrane
0.0731.93E-8SGTC_480382-0018 55.0 μMChemDiv (Drug-like library)7625960.0793651
0.0655.38E-7SGTC_29989066762 71.4 μMChembridge (Drug-like library)49451960.19697RPP1 & pyrimidine depletion
0.0648.11E-7SGTC_227fluconazole 33.5 μMMiscellaneous33650.057971azole & statin
0.0631.18E-6SGTC_1067clotrimazole 1.4 μMNIH Clinical Collection28120.147541azole & statin
0.0603.83E-6SGTC_29979067075 71.4 μMChembridge (Drug-like library)272098190.147059unfolded protein response
0.0604.13E-6SGTC_2685mycophenolic acid 18.9 μMMiscellaneous4465410.075RNA pol III & RNase P/MRP
0.0595.04E-6SGTC_21375261020 140.0 μMChembridge (Fragment library)7636550.104478
0.0595.40E-6SGTC_30459092950 49.5 μMChembridge (Drug-like library)441204320.234375
0.0595.43E-6SGTC_1980st071811 45.7 μMTimTec (Natural product derivative library)49748500.119048RPP1 & pyrimidine depletion
0.0596.61E-6SGTC_29157971052 15.2 μMChembridge (Drug-like library)57415580.0493827
0.0586.94E-6SGTC_32619137475 49.5 μMChembridge (Drug-like library)170276900.0641026RPP1 & pyrimidine depletion
0.0589.75E-6SGTC_1835st008666 49.2 μMTimTec (Natural product derivative library)6904550.396226
0.0561.53E-5SGTC_13271436-0082 2.2 μMChemDiv (Drug-like library)159922050.125

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_2835900658871.43 μM0.6888892345538Chembridge (Drug-like library)300.782683.27912
SGTC_3113912349849.47 μM0.6808518897275Chembridge (Drug-like library)308.417363.80812
SGTC_3177910750649.47 μM0.648897194Chembridge (Drug-like library)314.809263.76512ERG2
SGTC_3178910750649.47 μM0.648897194Chembridge (Drug-like library)314.809263.76512
SGTC_3179910780149.47 μM0.644818259Chembridge (Drug-like library)318.7731433.48413
SGTC_2895905444758.44 μM0.5957452697450Chembridge (Drug-like library)343.850483.16113
SGTC_1300136-0219337.66 μM0.42234244ChemDiv (Drug-like library)296.148643.77812
SGTC_74000s-0487160.28 μM0.403846677548ChemDiv (Drug-like library)277.702982.87223
SGTC_2893904848825.97 μM0.46467338Chembridge (Drug-like library)337.458583.46913fatty acid desaturase (OLE1)
SGTC_2838900268771.43 μM0.3965526457060Chembridge (Drug-like library)357.877063.64713fatty acid desaturase (OLE1)
SGTC_1835st00866649.2 μM0.396226690455TimTec (Natural product derivative library)306.186524.2301