9067767

1-(3-chlorophenyl)-3-(2-thiophen-2-ylethyl)urea

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2995
Screen concentration 71.4 μM
Source Chembridge (Drug-like library)
PubChem CID 11673567
SMILES C1=CC(=CC(=C1)Cl)NC(=O)NCCC2=CC=CS2
Standardized SMILES Clc1cccc(NC(=O)NCCc2cccs2)c1
Molecular weight 280.7731
ALogP 3.18
H-bond donor count 2
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.5
% growth inhibition (Hom. pool) 6.24


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 11673567
Download HIP data (tab-delimited text)  (excel)
Gene:CDC53(YDL132W)|FD-Score:-3.54|P-value:2.01E-4|Clearance:0||SGD DESC:Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation Gene:FCF2(YLR051C)|FD-Score:4.28|P-value:9.23E-6|Clearance:0.44||SGD DESC:Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon Gene:GCD2(YGR083C)|FD-Score:-4.41|P-value:5.14E-6|Clearance:0||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:IRR1(YIL026C)|FD-Score:4.12|P-value:1.90E-5|Clearance:0.44||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:NOC2(YOR206W)|FD-Score:3.68|P-value:1.16E-4|Clearance:0.28||SGD DESC:Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors Gene:NOP14(YDL148C)|FD-Score:3.2|P-value:6.87E-4|Clearance:0.02||SGD DESC:Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:NUP1(YOR098C)|FD-Score:3.4|P-value:3.32E-4|Clearance:0.17||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; possible karyopherin release factor that accelerates release of karyopherin-cargo complexes after transport across NPC; both NUP1 and NUP60 are homologous to human NUP153 Gene:PRP3(YDR473C)|FD-Score:-3.84|P-value:6.08E-5|Clearance:0||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RER2(YBR002C)|FD-Score:3.18|P-value:7.25E-4|Clearance:0.2||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RFA1(YAR007C)|FD-Score:-3.38|P-value:3.64E-4|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination Gene:RIB2(YOL066C)|FD-Score:-3.48|P-value:2.55E-4|Clearance:0||SGD DESC:Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs Gene:SEC31(YDL195W)|FD-Score:-3.22|P-value:6.34E-4|Clearance:0||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SNU56(YDR240C)|FD-Score:-3.9|P-value:4.83E-5|Clearance:0||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex Gene:SSY1(YDR160W)|FD-Score:3.23|P-value:6.20E-4|Clearance:0.03||SGD DESC:Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes Gene:YKL083W(YKL083W_d)|FD-Score:3.41|P-value:3.29E-4|Clearance:0||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene RRP14 Gene:YPR142C(YPR142C_d)|FD-Score:-3.1|P-value:9.66E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing Gene:CDC53(YDL132W)|FD-Score:-3.54|P-value:2.01E-4|Clearance:0||SGD DESC:Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation Gene:FCF2(YLR051C)|FD-Score:4.28|P-value:9.23E-6|Clearance:0.44||SGD DESC:Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon Gene:GCD2(YGR083C)|FD-Score:-4.41|P-value:5.14E-6|Clearance:0||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:IRR1(YIL026C)|FD-Score:4.12|P-value:1.90E-5|Clearance:0.44||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:NOC2(YOR206W)|FD-Score:3.68|P-value:1.16E-4|Clearance:0.28||SGD DESC:Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors Gene:NOP14(YDL148C)|FD-Score:3.2|P-value:6.87E-4|Clearance:0.02||SGD DESC:Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:NUP1(YOR098C)|FD-Score:3.4|P-value:3.32E-4|Clearance:0.17||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; possible karyopherin release factor that accelerates release of karyopherin-cargo complexes after transport across NPC; both NUP1 and NUP60 are homologous to human NUP153 Gene:PRP3(YDR473C)|FD-Score:-3.84|P-value:6.08E-5|Clearance:0||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RER2(YBR002C)|FD-Score:3.18|P-value:7.25E-4|Clearance:0.2||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RFA1(YAR007C)|FD-Score:-3.38|P-value:3.64E-4|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination Gene:RIB2(YOL066C)|FD-Score:-3.48|P-value:2.55E-4|Clearance:0||SGD DESC:Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs Gene:SEC31(YDL195W)|FD-Score:-3.22|P-value:6.34E-4|Clearance:0||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SNU56(YDR240C)|FD-Score:-3.9|P-value:4.83E-5|Clearance:0||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex Gene:SSY1(YDR160W)|FD-Score:3.23|P-value:6.20E-4|Clearance:0.03||SGD DESC:Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes Gene:YKL083W(YKL083W_d)|FD-Score:3.41|P-value:3.29E-4|Clearance:0||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene RRP14 Gene:YPR142C(YPR142C_d)|FD-Score:-3.1|P-value:9.66E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 11673567
Download HOP data (tab-delimited text)  (excel)
Gene:AFT1(YGL071W)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AGA2(YGL032C)|FD-Score:3.12|P-value:8.95E-4||SGD DESC:Adhesion subunit of a-agglutinin of a-cells, C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds Gene:AIM33(YML087C)|FD-Score:-3.11|P-value:9.51E-4||SGD DESC:Putative protein of unknown function, highly conserved across species; homolog of human CYB5R4; null mutant displays reduced frequency of mitochondrial genome loss; AIM33 has a paralog, PGA3, that arose from the whole genome duplication Gene:ALD6(YPL061W)|FD-Score:-3.37|P-value:3.77E-4||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:APL3(YBL037W)|FD-Score:3.2|P-value:6.87E-4||SGD DESC:Alpha-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport Gene:ARD1(YHR013C)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Subunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; acetylates many proteins and thus affects telomeric silencing, cell cycle, heat-shock resistance, mating, and sporulation; human Ard1p levels are elevated in cancer cells; protein abundance increases in response to DNA replication stress Gene:ARG82(YDR173C)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ATG10(YLL042C)|FD-Score:-3.23|P-value:6.29E-4||SGD DESC:Conserved E2-like conjugating enzyme that mediates formation of the Atg12p-Atg5p conjugate, which is a critical step in autophagy Gene:AVT4(YNL101W)|FD-Score:3.86|P-value:5.66E-5||SGD DESC:Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:AXL1(YPR122W)|FD-Score:3.22|P-value:6.30E-4||SGD DESC:Haploid specific endoprotease that performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells Gene:BSC4(YNL269W)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p Gene:BUB3(YOR026W)|FD-Score:5.12|P-value:1.53E-7||SGD DESC:Kinetochore checkpoint WD40 repeat protein that localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p Gene:CLN3(YAL040C)|FD-Score:3.96|P-value:3.74E-5||SGD DESC:G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis Gene:CSH1(YBR161W)|FD-Score:6.29|P-value:1.62E-10||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication Gene:DDI3(YNL335W_p)|FD-Score:-3.1|P-value:9.78E-4||SGD DESC:Protein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants Gene:DMA2(YNL116W)|FD-Score:-3.44|P-value:2.94E-4||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma1p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ortholog of human RNF8 protein, with sequence similarity to human Chfr; contains FHA and RING finger domains Gene:DUF1(YOL087C)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:EMP47(YFL048C)|FD-Score:-3.1|P-value:9.82E-4||SGD DESC:Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport Gene:FRE7(YOL152W)|FD-Score:-3.59|P-value:1.64E-4||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels Gene:GLK1(YCL040W)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication Gene:GLY1(YEL046C)|FD-Score:-3.19|P-value:7.04E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GMH1(YKR030W)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Golgi membrane protein of unknown function, interacts with Gea1p and Gea2p; required for localization of Gea2p; computational analysis suggests a possible role in either cell wall synthesis or protein-vacuolar targeting Gene:HPF1(YOL155C)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Haze-protective mannoprotein that reduces the particle size of aggregated proteins in white wines Gene:IRC14(YOR135C_d)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:IRC6(YFR043C)|FD-Score:-7.12|P-value:5.28E-13||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:LRP1(YHR081W)|FD-Score:-4.35|P-value:6.88E-6||SGD DESC:Nuclear exosome-associated nucleic acid binding protein; involved in RNA processing, surveillance, degradation, tethering, and export; homolog of mammalian nuclear matrix protein C1D involved in regulation of DNA repair and recombination Gene:MAM3(YOL060C)|FD-Score:5.19|P-value:1.03E-7||SGD DESC:Protein required for normal mitochondrial morphology, has similarity to hemolysins Gene:MCH4(YOL119C)|FD-Score:-4.06|P-value:2.46E-5||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MHR1(YDR296W)|FD-Score:7.06|P-value:8.57E-13||SGD DESC:Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress Gene:MMR1(YLR190W)|FD-Score:4.73|P-value:1.14E-6||SGD DESC:Phosphorylated protein of the mitochondrial outer membrane, localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p Gene:MRPL22(YNL177C)|FD-Score:3.84|P-value:6.08E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL51(YPR100W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSH3(YCR092C)|FD-Score:6.5|P-value:4.04E-11||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:MTC4(YBR255W)|FD-Score:3.11|P-value:9.20E-4||SGD DESC:Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 Gene:OXA1(YER154W)|FD-Score:3.72|P-value:9.94E-5||SGD DESC:Mitochondrial inner membrane insertase, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; conserved from bacteria to animals Gene:PEX13(YLR191W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Integral peroxisomal membrane protein required for translocation of peroxisomal matrix proteins, interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p, forms a complex with Pex14p and Pex17p Gene:PHO87(YCR037C)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication Gene:PPE1(YHR075C)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Protein with carboxyl methyl esterase activity that may have a role in demethylation of the phosphoprotein phosphatase catalytic subunit; also identified as a small subunit mitochondrial ribosomal protein Gene:PUS7(YOR243C)|FD-Score:5.03|P-value:2.45E-7||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:RDS1(YCR106W)|FD-Score:4.91|P-value:4.53E-7||SGD DESC:Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide Gene:REV7(YIL139C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RGD2(YFL047W)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RPL35A(YDL191W)|FD-Score:4.73|P-value:1.13E-6||SGD DESC:Ribosomal 60S subunit protein L35A; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35A has a paralog, RPL35B, that arose from the whole genome duplication Gene:SIF2(YBR103W)|FD-Score:4.64|P-value:1.75E-6||SGD DESC:WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus Gene:SIN4(YNL236W)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SKG3(YLR187W_p)|FD-Score:-3.97|P-value:3.67E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication Gene:SLH1(YGR271W)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SNF2(YOR290C)|FD-Score:4.75|P-value:1.03E-6||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SPS18(YNL204C)|FD-Score:-3.4|P-value:3.32E-4||SGD DESC:Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation Gene:SRP40(YKR092C)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Nucleolar, serine-rich protein with a role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140 Gene:TAE2(YPL009C)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Protein of unknown function, involved in protein translation; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:TRP4(YDR354W)|FD-Score:4.08|P-value:2.27E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:-4.24|P-value:1.13E-5||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TVP38(YKR088C)|FD-Score:5.8|P-value:3.27E-9||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:UBC11(YOR339C)|FD-Score:6.56|P-value:2.70E-11||SGD DESC:Ubiquitin-conjugating enzyme most similar in sequence to Xenopus ubiquitin-conjugating enzyme E2-C, but not a true functional homolog of this E2; unlike E2-C, not required for the degradation of mitotic cyclin Clb2 Gene:UBX2(YML013W)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:VMA5(YKL080W)|FD-Score:5.62|P-value:9.67E-9||SGD DESC:Subunit C of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:YAR028W(YAR028W_p)|FD-Score:4.07|P-value:2.37E-5||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YBL044W(YBL044W_p)|FD-Score:-3.79|P-value:7.49E-5||SGD DESC:Putative protein of unknown function; YBL044W is not an essential protein Gene:YBL071C-B(YBL071C-B_p)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCK3(YER123W)|FD-Score:-5.47|P-value:2.26E-8||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YDR124W(YDR124W_p)|FD-Score:-4.05|P-value:2.58E-5||SGD DESC:Putative protein of unknown function; non-essential gene; expression is strongly induced by alpha factor Gene:YGL159W(YGL159W_p)|FD-Score:-5.31|P-value:5.40E-8||SGD DESC:Putative protein of unknown function; deletion mutant has no detectable phenotype Gene:YHI9(YHR029C)|FD-Score:-3.22|P-value:6.45E-4||SGD DESC:Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production Gene:YLL007C(YLL007C_p)|FD-Score:-4.63|P-value:1.80E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YLL059C(YLL059C_d)|FD-Score:5.14|P-value:1.36E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR149C(YLR149C_p)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YMR245W(YMR245W_d)|FD-Score:3.56|P-value:1.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR029C(YNR029C_p)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Putative protein of unknown function, deletion confers reduced fitness in saline Gene:YOX1(YML027W)|FD-Score:-4.29|P-value:8.81E-6||SGD DESC:Homeobox transcriptional repressor; binds to Mcm1p and to early cell cycle boxes (ECBs) in the promoters of cell cycle-regulated genes expressed in M/G1 phase; expression is cell cycle-regulated; potential Cdc28p substrate; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOX1 has a paralog, YHP1, that arose from the whole genome duplication Gene:YPR098C(YPR098C)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YPT10(YBR264C)|FD-Score:4.38|P-value:5.88E-6||SGD DESC:Rab family GTP-binding protein that contains the PEST signal sequence specific for proteolytic enzymes; may be involved in vesicular transport; overexpression leads to accumulation of Golgi-like cisternae with budding vesicles Gene:YPT35(YHR105W)|FD-Score:3.76|P-value:8.58E-5||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport Gene:AFT1(YGL071W)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AGA2(YGL032C)|FD-Score:3.12|P-value:8.95E-4||SGD DESC:Adhesion subunit of a-agglutinin of a-cells, C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds Gene:AIM33(YML087C)|FD-Score:-3.11|P-value:9.51E-4||SGD DESC:Putative protein of unknown function, highly conserved across species; homolog of human CYB5R4; null mutant displays reduced frequency of mitochondrial genome loss; AIM33 has a paralog, PGA3, that arose from the whole genome duplication Gene:ALD6(YPL061W)|FD-Score:-3.37|P-value:3.77E-4||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:APL3(YBL037W)|FD-Score:3.2|P-value:6.87E-4||SGD DESC:Alpha-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport Gene:ARD1(YHR013C)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Subunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; acetylates many proteins and thus affects telomeric silencing, cell cycle, heat-shock resistance, mating, and sporulation; human Ard1p levels are elevated in cancer cells; protein abundance increases in response to DNA replication stress Gene:ARG82(YDR173C)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ATG10(YLL042C)|FD-Score:-3.23|P-value:6.29E-4||SGD DESC:Conserved E2-like conjugating enzyme that mediates formation of the Atg12p-Atg5p conjugate, which is a critical step in autophagy Gene:AVT4(YNL101W)|FD-Score:3.86|P-value:5.66E-5||SGD DESC:Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:AXL1(YPR122W)|FD-Score:3.22|P-value:6.30E-4||SGD DESC:Haploid specific endoprotease that performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells Gene:BSC4(YNL269W)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p Gene:BUB3(YOR026W)|FD-Score:5.12|P-value:1.53E-7||SGD DESC:Kinetochore checkpoint WD40 repeat protein that localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p Gene:CLN3(YAL040C)|FD-Score:3.96|P-value:3.74E-5||SGD DESC:G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis Gene:CSH1(YBR161W)|FD-Score:6.29|P-value:1.62E-10||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication Gene:DDI3(YNL335W_p)|FD-Score:-3.1|P-value:9.78E-4||SGD DESC:Protein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants Gene:DMA2(YNL116W)|FD-Score:-3.44|P-value:2.94E-4||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma1p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ortholog of human RNF8 protein, with sequence similarity to human Chfr; contains FHA and RING finger domains Gene:DUF1(YOL087C)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:EMP47(YFL048C)|FD-Score:-3.1|P-value:9.82E-4||SGD DESC:Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport Gene:FRE7(YOL152W)|FD-Score:-3.59|P-value:1.64E-4||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels Gene:GLK1(YCL040W)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication Gene:GLY1(YEL046C)|FD-Score:-3.19|P-value:7.04E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GMH1(YKR030W)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Golgi membrane protein of unknown function, interacts with Gea1p and Gea2p; required for localization of Gea2p; computational analysis suggests a possible role in either cell wall synthesis or protein-vacuolar targeting Gene:HPF1(YOL155C)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Haze-protective mannoprotein that reduces the particle size of aggregated proteins in white wines Gene:IRC14(YOR135C_d)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:IRC6(YFR043C)|FD-Score:-7.12|P-value:5.28E-13||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:LRP1(YHR081W)|FD-Score:-4.35|P-value:6.88E-6||SGD DESC:Nuclear exosome-associated nucleic acid binding protein; involved in RNA processing, surveillance, degradation, tethering, and export; homolog of mammalian nuclear matrix protein C1D involved in regulation of DNA repair and recombination Gene:MAM3(YOL060C)|FD-Score:5.19|P-value:1.03E-7||SGD DESC:Protein required for normal mitochondrial morphology, has similarity to hemolysins Gene:MCH4(YOL119C)|FD-Score:-4.06|P-value:2.46E-5||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MHR1(YDR296W)|FD-Score:7.06|P-value:8.57E-13||SGD DESC:Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress Gene:MMR1(YLR190W)|FD-Score:4.73|P-value:1.14E-6||SGD DESC:Phosphorylated protein of the mitochondrial outer membrane, localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p Gene:MRPL22(YNL177C)|FD-Score:3.84|P-value:6.08E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL51(YPR100W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSH3(YCR092C)|FD-Score:6.5|P-value:4.04E-11||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:MTC4(YBR255W)|FD-Score:3.11|P-value:9.20E-4||SGD DESC:Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 Gene:OXA1(YER154W)|FD-Score:3.72|P-value:9.94E-5||SGD DESC:Mitochondrial inner membrane insertase, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; conserved from bacteria to animals Gene:PEX13(YLR191W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Integral peroxisomal membrane protein required for translocation of peroxisomal matrix proteins, interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p, forms a complex with Pex14p and Pex17p Gene:PHO87(YCR037C)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication Gene:PPE1(YHR075C)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Protein with carboxyl methyl esterase activity that may have a role in demethylation of the phosphoprotein phosphatase catalytic subunit; also identified as a small subunit mitochondrial ribosomal protein Gene:PUS7(YOR243C)|FD-Score:5.03|P-value:2.45E-7||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:RDS1(YCR106W)|FD-Score:4.91|P-value:4.53E-7||SGD DESC:Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide Gene:REV7(YIL139C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RGD2(YFL047W)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RPL35A(YDL191W)|FD-Score:4.73|P-value:1.13E-6||SGD DESC:Ribosomal 60S subunit protein L35A; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35A has a paralog, RPL35B, that arose from the whole genome duplication Gene:SIF2(YBR103W)|FD-Score:4.64|P-value:1.75E-6||SGD DESC:WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus Gene:SIN4(YNL236W)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SKG3(YLR187W_p)|FD-Score:-3.97|P-value:3.67E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication Gene:SLH1(YGR271W)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SNF2(YOR290C)|FD-Score:4.75|P-value:1.03E-6||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SPS18(YNL204C)|FD-Score:-3.4|P-value:3.32E-4||SGD DESC:Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation Gene:SRP40(YKR092C)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Nucleolar, serine-rich protein with a role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140 Gene:TAE2(YPL009C)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Protein of unknown function, involved in protein translation; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:TRP4(YDR354W)|FD-Score:4.08|P-value:2.27E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:-4.24|P-value:1.13E-5||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TVP38(YKR088C)|FD-Score:5.8|P-value:3.27E-9||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:UBC11(YOR339C)|FD-Score:6.56|P-value:2.70E-11||SGD DESC:Ubiquitin-conjugating enzyme most similar in sequence to Xenopus ubiquitin-conjugating enzyme E2-C, but not a true functional homolog of this E2; unlike E2-C, not required for the degradation of mitotic cyclin Clb2 Gene:UBX2(YML013W)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:VMA5(YKL080W)|FD-Score:5.62|P-value:9.67E-9||SGD DESC:Subunit C of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:YAR028W(YAR028W_p)|FD-Score:4.07|P-value:2.37E-5||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YBL044W(YBL044W_p)|FD-Score:-3.79|P-value:7.49E-5||SGD DESC:Putative protein of unknown function; YBL044W is not an essential protein Gene:YBL071C-B(YBL071C-B_p)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCK3(YER123W)|FD-Score:-5.47|P-value:2.26E-8||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YDR124W(YDR124W_p)|FD-Score:-4.05|P-value:2.58E-5||SGD DESC:Putative protein of unknown function; non-essential gene; expression is strongly induced by alpha factor Gene:YGL159W(YGL159W_p)|FD-Score:-5.31|P-value:5.40E-8||SGD DESC:Putative protein of unknown function; deletion mutant has no detectable phenotype Gene:YHI9(YHR029C)|FD-Score:-3.22|P-value:6.45E-4||SGD DESC:Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production Gene:YLL007C(YLL007C_p)|FD-Score:-4.63|P-value:1.80E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YLL059C(YLL059C_d)|FD-Score:5.14|P-value:1.36E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR149C(YLR149C_p)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YMR245W(YMR245W_d)|FD-Score:3.56|P-value:1.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR029C(YNR029C_p)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Putative protein of unknown function, deletion confers reduced fitness in saline Gene:YOX1(YML027W)|FD-Score:-4.29|P-value:8.81E-6||SGD DESC:Homeobox transcriptional repressor; binds to Mcm1p and to early cell cycle boxes (ECBs) in the promoters of cell cycle-regulated genes expressed in M/G1 phase; expression is cell cycle-regulated; potential Cdc28p substrate; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOX1 has a paralog, YHP1, that arose from the whole genome duplication Gene:YPR098C(YPR098C)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YPT10(YBR264C)|FD-Score:4.38|P-value:5.88E-6||SGD DESC:Rab family GTP-binding protein that contains the PEST signal sequence specific for proteolytic enzymes; may be involved in vesicular transport; overexpression leads to accumulation of Golgi-like cisternae with budding vesicles Gene:YPT35(YHR105W)|FD-Score:3.76|P-value:8.58E-5||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR051C4.289.23E-60.44FCF2Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon
YIL026C4.121.90E-50.44IRR1Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability
YOR206W3.681.16E-40.28NOC2Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors
YKL083W_d3.413.29E-40.00YKL083W_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14
YOR098C3.403.32E-40.17NUP1FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; possible karyopherin release factor that accelerates release of karyopherin-cargo complexes after transport across NPC; both NUP1 and NUP60 are homologous to human NUP153
YDR160W3.236.20E-40.03SSY1Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes
YDL148C3.206.87E-40.02NOP14Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
YBR002C3.187.25E-40.20RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YFL017W-A2.990.001410.06SMX2Core Sm protein Sm G; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx3p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm G
YPL082C2.930.001710.00MOT1Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA
YLR195C2.920.001730.00NMT1N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction
YDR356W2.920.001750.02SPC110Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner
YGR098C2.900.001850.01ESP1Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress
YGR082W2.890.001910.16TOM20Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins
YDL108W2.730.003130.02KIN28Serine/threonine protein kinase, subunit of the transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR296W7.068.57E-13MHR1Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress
YOR339C6.562.70E-11UBC11Ubiquitin-conjugating enzyme most similar in sequence to Xenopus ubiquitin-conjugating enzyme E2-C, but not a true functional homolog of this E2; unlike E2-C, not required for the degradation of mitotic cyclin Clb2
YCR092C6.504.04E-11MSH3Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability
YBR161W6.291.62E-10CSH1Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication
YKR088C5.803.27E-9TVP38Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern
YKL080W5.629.67E-9VMA5Subunit C of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane
YOL060C5.191.03E-7MAM3Protein required for normal mitochondrial morphology, has similarity to hemolysins
YLL059C_d5.141.36E-7YLL059C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YOR026W5.121.53E-7BUB3Kinetochore checkpoint WD40 repeat protein that localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p
YOR243C5.032.45E-7PUS7Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria
YCR106W4.914.53E-7RDS1Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide
YOR290C4.751.03E-6SNF2Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p
YDL191W4.731.13E-6RPL35ARibosomal 60S subunit protein L35A; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35A has a paralog, RPL35B, that arose from the whole genome duplication
YLR190W4.731.14E-6MMR1Phosphorylated protein of the mitochondrial outer membrane, localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p
YBR103W4.641.75E-6SIF2WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus

GO enrichment analysis for SGTC_2995
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0863.86E-11SGTC_100279-0212 42.6 μMChemDiv (Drug-like library)398510.103448TRP & mitochondrial translation
0.0814.00E-10SGTC_190279-0214 38.4 μMChemDiv (Drug-like library)35662740.103448TRP & mitochondrial translation
0.0781.79E-9SGTC_2741miltefosine 3.0 μMMiscellaneous35990.0307692
0.0714.18E-8SGTC_30919116372 49.5 μMChembridge (Drug-like library)171697930.238095
0.0707.86E-8SGTC_2669eugenol 100.0 μMMicrosource (Natural product library)33140.0806452
0.0672.93E-7SGTC_1116jasmonic acid  1.6 μMTimTec (Natural product library)52811660.0735294
0.0663.55E-7SGTC_31839108214 49.5 μMChembridge (Drug-like library)49748310.0588235RPP1 & pyrimidine depletion
0.0648.93E-7SGTC_33489149464 3.5 μMChembridge (Drug-like library)19514420.0722892
0.0603.69E-6SGTC_9332914-0560 114.0 μMChemDiv (Drug-like library)4638870.15
0.0604.31E-6SGTC_5961155-0152 116.0 μMChemDiv (Drug-like library)2803130.0806452TRP & mitochondrial translation
0.0588.86E-6SGTC_22887946289 167.3 μMChembridge (Fragment library)12460520.0985916
0.0571.27E-5SGTC_305-(2-thienylidene)-Rhodanine 28.7 μMChemDiv (Drug-like library)12411320.163934
0.0543.20E-5SGTC_7670929-0023 86.0 μMChemDiv (Drug-like library)12045630.0857143TRP & mitochondrial translation
0.0543.29E-5SGTC_13802291-0730 164.0 μMChemDiv (Drug-like library)29991910.0512821
0.0525.54E-5SGTC_22637948595 193.3 μMChembridge (Fragment library)29713710.193548

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_12550566-0045221 μM0.4230772788469ChemDiv (Drug-like library)304.728323.04223excess fatty acid
SGTC_2942905277762.25 μM0.3773586469503Chembridge (Drug-like library)284.1131234.14912
SGTC_3092911724849.47 μM0.37096817141857Chembridge (Drug-like library)346.808063.52923cell wall signaling
SGTC_2392N-(3-chlorophenyl)-6-oxo-1H-pyridine-3-carboxamide200 μM0.363636803109Chembridge (Fragment library)248.665061.08522
SGTC_2787528454071.43 μM0.363636736958Chembridge (Drug-like library)291.729563.24613
SGTC_1190121-004324.82 μM0.3571431092378ChemDiv (Drug-like library)405.469124.53322mitochondrial stress
SGTC_2890904672958.44 μM0.357143903329Chembridge (Drug-like library)270.114662.88412Golgi
SGTC_7161683-5115658 μM0.350877723597ChemDiv (Drug-like library)303.740263.48313
SGTC_8820843-0131351 μM0.3508775425421ChemDiv (Drug-like library)278.757243.33613
SGTC_2847900566171.43 μM0.3508772991449Chembridge (Drug-like library)303.740263.42913