5-(2-thienylidene)-Rhodanine

(5E)-2-sulfanylidene-5-(thiophen-2-ylmethylidene)-1,3-thiazolidin-4-one

A phosphatase inhibitor.

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_30
Screen concentration 28.7 μM
Source ChemDiv (Drug-like library)
PubChem CID 1241132
SMILES C1=CSC(=C1)C=C2C(=O)NC(=S)S2
Standardized SMILES O=C1NC(=S)SC1=Cc2cccs2
Molecular weight 227.3264
ALogP 2.79
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 19.56
% growth inhibition (Hom. pool) 10.86


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1241132
Download HIP data (tab-delimited text)  (excel)
Gene:APC1(YNL172W)|FD-Score:3.19|P-value:7.17E-4|Clearance:0.15||SGD DESC:Largest subunit of the Anaphase-Promoting Complex/Cyclosome; APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:LSM2(YBL026W)|FD-Score:-3.27|P-value:5.42E-4|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MOB2(YFL034C-B)|FD-Score:-3.39|P-value:3.54E-4|Clearance:0||SGD DESC:Activator of Cbk1p kinase; component of the RAM signaling network that regulates cellular polarity and morphogenesis; activation of Cbk1p facilitates the Ace2p-dependent daughter cell-specific transcription of genes involved in cell separation; similar to Mob1p Gene:MPE1(YKL059C)|FD-Score:4.38|P-value:5.92E-6|Clearance:0.68||SGD DESC:Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif Gene:NUS1(YDL193W)|FD-Score:4.28|P-value:9.56E-6|Clearance:0.68||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:ORC6(YHR118C)|FD-Score:3.59|P-value:1.65E-4|Clearance:0.4||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p Gene:RER2(YBR002C)|FD-Score:4.52|P-value:3.16E-6|Clearance:0.68||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RPL32(YBL092W)|FD-Score:-3.57|P-value:1.78E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:SRP54(YPR088C)|FD-Score:-5.91|P-value:1.67E-9|Clearance:0||SGD DESC:Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain Gene:YGR265W(YGR265W_d)|FD-Score:-3.15|P-value:8.13E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase Gene:APC1(YNL172W)|FD-Score:3.19|P-value:7.17E-4|Clearance:0.15||SGD DESC:Largest subunit of the Anaphase-Promoting Complex/Cyclosome; APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:LSM2(YBL026W)|FD-Score:-3.27|P-value:5.42E-4|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MOB2(YFL034C-B)|FD-Score:-3.39|P-value:3.54E-4|Clearance:0||SGD DESC:Activator of Cbk1p kinase; component of the RAM signaling network that regulates cellular polarity and morphogenesis; activation of Cbk1p facilitates the Ace2p-dependent daughter cell-specific transcription of genes involved in cell separation; similar to Mob1p Gene:MPE1(YKL059C)|FD-Score:4.38|P-value:5.92E-6|Clearance:0.68||SGD DESC:Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif Gene:NUS1(YDL193W)|FD-Score:4.28|P-value:9.56E-6|Clearance:0.68||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:ORC6(YHR118C)|FD-Score:3.59|P-value:1.65E-4|Clearance:0.4||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p Gene:RER2(YBR002C)|FD-Score:4.52|P-value:3.16E-6|Clearance:0.68||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RPL32(YBL092W)|FD-Score:-3.57|P-value:1.78E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:SRP54(YPR088C)|FD-Score:-5.91|P-value:1.67E-9|Clearance:0||SGD DESC:Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain Gene:YGR265W(YGR265W_d)|FD-Score:-3.15|P-value:8.13E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1241132
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ADE12(YNL220W)|FD-Score:-6.99|P-value:1.34E-12||SGD DESC:Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence Gene:FMP41(YNL168C_p)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GCN2(YDR283C)|FD-Score:3.85|P-value:5.80E-5||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:4.39|P-value:5.77E-6||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:4.73|P-value:1.13E-6||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:HMS1(YOR032C)|FD-Score:3.8|P-value:7.20E-5||SGD DESC:Basic helix-loop-helix (bHLH) protein with similarity to myc-family transcription factors; overexpression confers hyperfilamentous growth and suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant Gene:HST2(YPL015C)|FD-Score:3.2|P-value:6.96E-4||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:MGA2(YIR033W)|FD-Score:-3.18|P-value:7.43E-4||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:PHO4(YFR034C)|FD-Score:-3.1|P-value:9.53E-4||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:PLB2(YMR006C)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; displays transacylase activity in vitro; overproduction confers resistance to lysophosphatidylcholine Gene:SFB3(YHR098C)|FD-Score:3.73|P-value:9.56E-5||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SNF6(YHL025W)|FD-Score:-4.33|P-value:7.52E-6||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:TRP1(YDR007W)|FD-Score:12.2|P-value:1.48E-34||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP4(YDR354W)|FD-Score:11.9|P-value:8.67E-33||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:VMA13(YPR036W)|FD-Score:-3.4|P-value:3.32E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VMA7(YGR020C)|FD-Score:-3.14|P-value:8.46E-4||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS52(YDR484W)|FD-Score:-3.13|P-value:8.75E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:YDR008C(YDR008C_d)|FD-Score:5.24|P-value:8.11E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL152W(YIL152W_p)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Putative protein of unknown function Gene:YMR010W(YMR010W_p)|FD-Score:4.79|P-value:8.50E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR010W is not an essential gene; YMR010W mRNA is transcribed with ADI1 Gene:YMR158C-A(YMR158C-A_p)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Putative protein of unknown function, may contain a lipid attachment site; YMR158C-A is not an essential gene Gene:ACB1(YGR037C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ADE12(YNL220W)|FD-Score:-6.99|P-value:1.34E-12||SGD DESC:Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence Gene:FMP41(YNL168C_p)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GCN2(YDR283C)|FD-Score:3.85|P-value:5.80E-5||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:4.39|P-value:5.77E-6||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:4.73|P-value:1.13E-6||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:HMS1(YOR032C)|FD-Score:3.8|P-value:7.20E-5||SGD DESC:Basic helix-loop-helix (bHLH) protein with similarity to myc-family transcription factors; overexpression confers hyperfilamentous growth and suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant Gene:HST2(YPL015C)|FD-Score:3.2|P-value:6.96E-4||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:MGA2(YIR033W)|FD-Score:-3.18|P-value:7.43E-4||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:PHO4(YFR034C)|FD-Score:-3.1|P-value:9.53E-4||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:PLB2(YMR006C)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; displays transacylase activity in vitro; overproduction confers resistance to lysophosphatidylcholine Gene:SFB3(YHR098C)|FD-Score:3.73|P-value:9.56E-5||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SNF6(YHL025W)|FD-Score:-4.33|P-value:7.52E-6||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:TRP1(YDR007W)|FD-Score:12.2|P-value:1.48E-34||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP4(YDR354W)|FD-Score:11.9|P-value:8.67E-33||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:VMA13(YPR036W)|FD-Score:-3.4|P-value:3.32E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VMA7(YGR020C)|FD-Score:-3.14|P-value:8.46E-4||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS52(YDR484W)|FD-Score:-3.13|P-value:8.75E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:YDR008C(YDR008C_d)|FD-Score:5.24|P-value:8.11E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL152W(YIL152W_p)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Putative protein of unknown function Gene:YMR010W(YMR010W_p)|FD-Score:4.79|P-value:8.50E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR010W is not an essential gene; YMR010W mRNA is transcribed with ADI1 Gene:YMR158C-A(YMR158C-A_p)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Putative protein of unknown function, may contain a lipid attachment site; YMR158C-A is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR002C4.523.16E-60.69RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YKL059C4.385.92E-60.69MPE1Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif
YDL193W4.289.56E-60.69NUS1Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY
YHR118C3.591.65E-40.40ORC6Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p
YNL172W3.197.17E-40.15APC1Largest subunit of the Anaphase-Promoting Complex/Cyclosome; APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; localizes to nuclear foci that become diffuse upon DNA replication stress
YNL007C3.030.001210.06SIS1Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; not functionally redundant with Ydj1p due to due to substrate specificity; has similarity to bacterial DnaJ proteins; protein abundance increases in response to DNA replication stress
YER093C2.970.001480.07TSC11Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p), a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; involved in sphingolipid metabolism; contains a RasGEFN domain
YKL060C2.900.001870.22FBA1Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress
YOR294W2.680.003640.10RRS1Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice
YNL260C_p2.590.004850.08LTO1_pEssential protein that forms a complex with Rli1p and Yae1p; ortholog of human ORAOV1, which is overexpressed in solid tumors; inviability of null mutant under standard conditions is complemented by overexpression of ORAOV1; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species
YOR168W2.510.006090.02GLN4Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions
YJL087C2.490.006400.01TRL1tRNA ligase, required for tRNA splicing and for both splicing and translation of HAC1 mRNA in the UPR; has phosphodiesterase, polynucleotide kinase, and ligase activities; localized at the inner nuclear envelope and partially to polysomes
YDR454C2.470.006670.12GUK1Guanylate kinase, converts GMP to GDP; required for growth and mannose outer chain elongation of cell wall N-linked glycoproteins
YFL039C2.360.009140.01ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YLL011W2.350.009340.00SOF1Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR007W12.201.48E-34TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YDR354W11.908.67E-33TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YDR008C_d5.248.11E-8YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YMR010W_p4.798.50E-7YMR010W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR010W is not an essential gene; YMR010W mRNA is transcribed with ADI1
YKR026C4.731.13E-6GCN3Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression
YFR009W4.395.77E-6GCN20Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA
YDR283C3.855.80E-5GCN2Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control
YOR032C3.807.20E-5HMS1Basic helix-loop-helix (bHLH) protein with similarity to myc-family transcription factors; overexpression confers hyperfilamentous growth and suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant
YHR098C3.739.56E-5SFB3Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p
YGR037C3.611.54E-4ACB1Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress
YIL152W_p3.512.22E-4YIL152W_pPutative protein of unknown function
YNL168C_p3.383.61E-4FMP41_pPutative protein of unknown function; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YEL009C3.344.12E-4GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
YMR158C-A_p3.324.43E-4YMR158C-A_pPutative protein of unknown function, may contain a lipid attachment site; YMR158C-A is not an essential gene
YGL026C3.245.88E-4TRP5Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis

GO enrichment analysis for SGTC_30
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.4533.24E-297SGTC_511013-0126 23.4 μMChemDiv (Drug-like library)16278500.177419
0.4412.15E-279SGTC_24885268135 45.7 μMMiscellaneous12703560.454545
0.4392.90E-276SGTC_190279-0214 38.4 μMChemDiv (Drug-like library)35662740.0754717TRP & mitochondrial translation
0.4381.85E-275SGTC_23849036245 200.0 μMChembridge (Fragment library)64627660.0655738
0.4362.45E-272SGTC_18825615643 20.0 μMMiscellaneous22530750.171429TRP & mitochondrial translation
0.4319.56E-266SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.0606061
0.4051.80E-231SGTC_1801486-1293 102.8 μMChemDiv (Drug-like library)41405360.0666667
0.4038.10E-230SGTC_18835-benzylidenerhodanine 20.0 μMMiscellaneous12732100.578947TRP & mitochondrial translation
0.3859.76E-208SGTC_6770929-0063 136.0 μMChemDiv (Drug-like library)15395830.294118
0.3841.21E-206SGTC_24785763493 32.3 μMMiscellaneous13655760.38
0.3832.03E-205SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.288462
0.3822.49E-204SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.0606061
0.3813.26E-203SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.0512821
0.3753.67E-196SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0240964
0.3752.92E-196SGTC_18815652484 16.0 μMMiscellaneous22551290.157143TRP & mitochondrial translation

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_18835-benzylidenerhodanine20 μM0.5789471273210Miscellaneous221.298682.84113TRP & mitochondrial translation
SGTC_24545-benzylidenerhodanine10.36 μM0.5789471273210Miscellaneous221.298682.84113TRP & mitochondrial translation
SGTC_2065522159634.26 μM0.564103788581Chembridge (Fragment library)238.286141.71724
SGTC_21365328288199.3 μM0.512821262395Chembridge (Fragment library)222.220540.81724
SGTC_5231934-022213.7 μM0.4680851361595ChemDiv (Drug-like library)289.296653.78316
SGTC_2032518511225.99 μM0.4565225718189Chembridge (Fragment library)241.286781.54415
SGTC_2448518511227.17 μM0.4565225718189Chembridge (Fragment library)241.286781.54415
SGTC_2488526813545.7 μM0.4545451270356Miscellaneous247.335963.30913
SGTC_8900929-000647.2 μM0.448981378169ChemDiv (Drug-like library)267.324062.58325
SGTC_7670929-002386 μM0.43751204563ChemDiv (Drug-like library)279.334762.69615TRP & mitochondrial translation
SGTC_2472537765249.4 μM0.4255321610903Miscellaneous261.362543.75513TRP & mitochondrial translation
SGTC_20565235370200 μM0.4166675511916Chembridge (Fragment library)237.301381.57125