9076496

N-(4-methoxyphenyl)-4-piperidin-1-ylbenzamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3005
Screen concentration 71.4 μM
Source Chembridge (Drug-like library)
PubChem CID 21368410
SMILES COC1=CC=C(C=C1)NC(=O)C2=CC=C(C=C2)N3CCCCC3
Standardized SMILES COc1ccc(NC(=O)c2ccc(cc2)N3CCCCC3)cc1
Molecular weight 310.3902
ALogP 3.68
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.27
% growth inhibition (Hom. pool) 5.32


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 21368410
Download HIP data (tab-delimited text)  (excel)
Gene:ABD1(YBR236C)|FD-Score:-3.51|P-value:2.28E-4|Clearance:0||SGD DESC:Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA Gene:ASA1(YPR085C)|FD-Score:3.49|P-value:2.43E-4|Clearance:0.36||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1 Gene:CET1(YPL228W)|FD-Score:3.45|P-value:2.76E-4|Clearance:0.36||SGD DESC:Beta (RNA 5'-triphosphatase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CEG1, a guanylyltransferase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide Gene:FIP1(YJR093C)|FD-Score:3.09|P-value:9.99E-4|Clearance:0.08||SGD DESC:Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p Gene:FMN1(YDR236C)|FD-Score:-3.7|P-value:1.08E-4|Clearance:0||SGD DESC:Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane Gene:GLE1(YDL207W)|FD-Score:-3.22|P-value:6.39E-4|Clearance:0||SGD DESC:Cytoplasmic nucleoporin required for polyadenylated RNA export; not essential for protein import; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export Gene:HEM15(YOR176W)|FD-Score:3.76|P-value:8.64E-5|Clearance:0.36||SGD DESC:Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway Gene:MET4(YNL103W)|FD-Score:-3.32|P-value:4.53E-4|Clearance:0||SGD DESC:Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p Gene:SEC3(YER008C)|FD-Score:-4.02|P-value:2.91E-5|Clearance:0||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress Gene:ABD1(YBR236C)|FD-Score:-3.51|P-value:2.28E-4|Clearance:0||SGD DESC:Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA Gene:ASA1(YPR085C)|FD-Score:3.49|P-value:2.43E-4|Clearance:0.36||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1 Gene:CET1(YPL228W)|FD-Score:3.45|P-value:2.76E-4|Clearance:0.36||SGD DESC:Beta (RNA 5'-triphosphatase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CEG1, a guanylyltransferase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide Gene:FIP1(YJR093C)|FD-Score:3.09|P-value:9.99E-4|Clearance:0.08||SGD DESC:Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p Gene:FMN1(YDR236C)|FD-Score:-3.7|P-value:1.08E-4|Clearance:0||SGD DESC:Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane Gene:GLE1(YDL207W)|FD-Score:-3.22|P-value:6.39E-4|Clearance:0||SGD DESC:Cytoplasmic nucleoporin required for polyadenylated RNA export; not essential for protein import; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export Gene:HEM15(YOR176W)|FD-Score:3.76|P-value:8.64E-5|Clearance:0.36||SGD DESC:Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway Gene:MET4(YNL103W)|FD-Score:-3.32|P-value:4.53E-4|Clearance:0||SGD DESC:Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p Gene:SEC3(YER008C)|FD-Score:-4.02|P-value:2.91E-5|Clearance:0||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 21368410
Download HOP data (tab-delimited text)  (excel)
Gene:ACF4(YJR083C)|FD-Score:-3.35|P-value:4.00E-4||SGD DESC:Protein of unknown function, computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate Gene:AGX1(YFL030W)|FD-Score:3.18|P-value:7.31E-4||SGD DESC:Alanine:glyoxylate aminotransferase (AGT), catalyzes the synthesis of glycine from glyoxylate, which is one of three pathways for glycine biosynthesis in yeast; has similarity to mammalian and plant alanine:glyoxylate aminotransferases Gene:AIM19(YIL087C)|FD-Score:-3.37|P-value:3.80E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth Gene:ATG9(YDL149W)|FD-Score:-3.32|P-value:4.46E-4||SGD DESC:Transmembrane protein involved in forming Cvt and autophagic vesicles; cycles between the phagophore assembly site (PAS) and other cytosolic punctate structures, not found in autophagosomes; may be involved in membrane delivery to the PAS Gene:BAP3(YDR046C)|FD-Score:-3.44|P-value:2.93E-4||SGD DESC:Amino acid permease; involved in uptake of cysteine, leucine, isoleucine and valine; BAP3 has a paralog, BAP2, that arose from the whole genome duplication Gene:CUE4(YML101C)|FD-Score:-3.18|P-value:7.41E-4||SGD DESC:Protein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; CUE4 has a paralog, CUE1, that arose from the whole genome duplication Gene:FBP1(YLR377C)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Fructose-1,6-bisphosphatase, key regulatory enzyme in the gluconeogenesis pathway, required for glucose metabolism; undergoes either proteasome-mediated or autophagy-mediated degradation depending on growth conditions; interacts with Vid30p Gene:FMP25(YLR077W)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Mitochondrial inner membrane protein required for an early step in assembly of respiratory complex III (cytochrome bc1 complex); mRNA is targeted to mitochondria Gene:FYV6(YNL133C)|FD-Score:-3.39|P-value:3.53E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:GCN2(YDR283C)|FD-Score:-3.86|P-value:5.61E-5||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GLT1(YDL171C)|FD-Score:-3.53|P-value:2.10E-4||SGD DESC:NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source Gene:HNT2(YDR305C)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Dinucleoside triphosphate hydrolase; has similarity to the tumor suppressor FHIT and belongs to the histidine triad (HIT) superfamily of nucleotide-binding proteins Gene:HRQ1(YDR291W_p)|FD-Score:3.21|P-value:6.72E-4||SGD DESC:3'-5'DNA helicase that has DNA strand annealing activity; helicase activity is stimulated by fork structure and 3'-tail length of substrates; belongs to the widely conserved RecQ family of proteins which are involved in maintaining genomic integrity; similar to the human RecQ4p implicated in Rothmund-Thomson syndrome(RTS) Gene:ICL1(YER065C)|FD-Score:-3.54|P-value:2.00E-4||SGD DESC:Isocitrate lyase, catalyzes the formation of succinate and glyoxylate from isocitrate, a key reaction of the glyoxylate cycle; expression of ICL1 is induced by growth on ethanol and repressed by growth on glucose Gene:ISY1(YJR050W)|FD-Score:4.19|P-value:1.39E-5||SGD DESC:Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles Gene:MCM16(YPR046W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3 Gene:MDL2(YPL270W)|FD-Score:6.42|P-value:6.61E-11||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, required for respiratory growth at high temperature; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis Gene:MDR1(YGR100W)|FD-Score:3.09|P-value:9.96E-4||SGD DESC:Cytoplasmic GTPase-activating protein for Ypt/Rab transport GTPases Ypt6p, Ypt31p and Sec4p; involved in recycling of internalized proteins and regulation of Golgi secretory function Gene:MET12(YPL023C)|FD-Score:3.1|P-value:9.78E-4||SGD DESC:Protein with methylenetetrahydrofolate reductase (MTHFR) activity in vitro; null mutant has no phenotype and is prototrophic for methionine; MET13 encodes major isozyme of MTHFR Gene:MRP7(YNL005C)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NAM7(YMR080C)|FD-Score:3.19|P-value:7.18E-4||SGD DESC:ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress Gene:OPI8(YKR035C_d)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A Gene:PDR3(YBL005W)|FD-Score:4.61|P-value:2.02E-6||SGD DESC:Transcriptional activator of the pleiotropic drug resistance network; regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting sites known as PDREs (PDR responsive elements); post-translationally up-regulated in cells lacking a functional mitochondrial genome; PDR3 has a paralog, PDR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:PFK26(YIL107C)|FD-Score:-3.16|P-value:7.95E-4||SGD DESC:6-phosphofructo-2-kinase, inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate; has negligible fructose-2,6-bisphosphatase activity; transcriptional regulation involves protein kinase A Gene:PKH3(YDR466W)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PUN1(YLR414C)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:Plasma membrane protein with a role in cell wall integrity; co-localizes with Sur7p in punctate membrane patches; null mutant displays decreased thermotolerance; transcription induced upon cell wall damage and metal ion stress Gene:RPL38(YLR325C)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Ribosomal 60S subunit protein L38; homologous to mammalian ribosomal protein L38, no bacterial homolog Gene:RTK1(YDL025C)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress Gene:SCS3(YGL126W)|FD-Score:3.8|P-value:7.31E-5||SGD DESC:Protein required for inositol prototrophy; required for normal ER membrane biosynthesis; ortholog of the FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; disputed role in the synthesis of inositol phospholipids from inositol Gene:SFA1(YDL168W)|FD-Score:5.21|P-value:9.20E-8||SGD DESC:Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress Gene:SSA1(YAL005C)|FD-Score:4|P-value:3.14E-5||SGD DESC:ATPase involved in protein folding and NLS-directed nuclear transport; member of HSP70 family; forms chaperone complex with Ydj1p; localized to nucleus, cytoplasm, and cell wall; 98% identical with paralog Ssa2p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes; general targeting factor of Hsp104p to prion fibrils Gene:SWT21(YNL187W)|FD-Score:-3.26|P-value:5.56E-4||SGD DESC:Protein involved in mRNA splicing; contains a consensus nuclear export signal (NES) sequence similar to the consensus sequence recognized by Crm1p; interacts genetically with Prp40p and Tgs1p; contains WD40 repeats Gene:TAX4(YJL083W)|FD-Score:-3.54|P-value:1.98E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; TAX4 has a paralog, IRS4, that arose from the whole genome duplication Gene:TMS1(YDR105C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Vacuolar membrane protein of unknown function that is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance Gene:UBC7(YMR022W)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly Gene:YGL039W(YGL039W)|FD-Score:-3.22|P-value:6.35E-4||SGD DESC:Oxidoreductase shown to reduce carbonyl compounds to chiral alcohols Gene:YGR149W(YGR149W_p)|FD-Score:-3.32|P-value:4.51E-4||SGD DESC:Putative protein of unknown function; predicted to be an integal membrane protein Gene:YMR074C(YMR074C)|FD-Score:-3.17|P-value:7.50E-4||SGD DESC:Protein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YMR122C(YMR122C_d)|FD-Score:3.79|P-value:7.56E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR141C(YMR141C_d)|FD-Score:3.25|P-value:5.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL057W(YOL057W)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Dipeptidyl-peptidase III; cleaves dipeptides from the amino terminus of target proteins; highly active on synthetic substrate Arg-Arg-2-naphthylamide; mammalian ortholog may be a biomarker for some cancers Gene:YOR034C-A(YOR034C-A_p)|FD-Score:-4.01|P-value:3.00E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YPK2(YMR104C)|FD-Score:-3.24|P-value:5.90E-4||SGD DESC:Protein kinase with similarity to serine/threonine protein kinase Ypk1p; functionally redundant with YPK1 at the genetic level; participates in a signaling pathway required for optimal cell wall integrity; homolog of mammalian kinase SGK; YPK2 has a paralog, YPK1, that arose from the whole genome duplication Gene:YPR050C(YPR050C_d)|FD-Score:4.15|P-value:1.63E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W Gene:ACF4(YJR083C)|FD-Score:-3.35|P-value:4.00E-4||SGD DESC:Protein of unknown function, computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate Gene:AGX1(YFL030W)|FD-Score:3.18|P-value:7.31E-4||SGD DESC:Alanine:glyoxylate aminotransferase (AGT), catalyzes the synthesis of glycine from glyoxylate, which is one of three pathways for glycine biosynthesis in yeast; has similarity to mammalian and plant alanine:glyoxylate aminotransferases Gene:AIM19(YIL087C)|FD-Score:-3.37|P-value:3.80E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth Gene:ATG9(YDL149W)|FD-Score:-3.32|P-value:4.46E-4||SGD DESC:Transmembrane protein involved in forming Cvt and autophagic vesicles; cycles between the phagophore assembly site (PAS) and other cytosolic punctate structures, not found in autophagosomes; may be involved in membrane delivery to the PAS Gene:BAP3(YDR046C)|FD-Score:-3.44|P-value:2.93E-4||SGD DESC:Amino acid permease; involved in uptake of cysteine, leucine, isoleucine and valine; BAP3 has a paralog, BAP2, that arose from the whole genome duplication Gene:CUE4(YML101C)|FD-Score:-3.18|P-value:7.41E-4||SGD DESC:Protein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; CUE4 has a paralog, CUE1, that arose from the whole genome duplication Gene:FBP1(YLR377C)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Fructose-1,6-bisphosphatase, key regulatory enzyme in the gluconeogenesis pathway, required for glucose metabolism; undergoes either proteasome-mediated or autophagy-mediated degradation depending on growth conditions; interacts with Vid30p Gene:FMP25(YLR077W)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Mitochondrial inner membrane protein required for an early step in assembly of respiratory complex III (cytochrome bc1 complex); mRNA is targeted to mitochondria Gene:FYV6(YNL133C)|FD-Score:-3.39|P-value:3.53E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:GCN2(YDR283C)|FD-Score:-3.86|P-value:5.61E-5||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GLT1(YDL171C)|FD-Score:-3.53|P-value:2.10E-4||SGD DESC:NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source Gene:HNT2(YDR305C)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Dinucleoside triphosphate hydrolase; has similarity to the tumor suppressor FHIT and belongs to the histidine triad (HIT) superfamily of nucleotide-binding proteins Gene:HRQ1(YDR291W_p)|FD-Score:3.21|P-value:6.72E-4||SGD DESC:3'-5'DNA helicase that has DNA strand annealing activity; helicase activity is stimulated by fork structure and 3'-tail length of substrates; belongs to the widely conserved RecQ family of proteins which are involved in maintaining genomic integrity; similar to the human RecQ4p implicated in Rothmund-Thomson syndrome(RTS) Gene:ICL1(YER065C)|FD-Score:-3.54|P-value:2.00E-4||SGD DESC:Isocitrate lyase, catalyzes the formation of succinate and glyoxylate from isocitrate, a key reaction of the glyoxylate cycle; expression of ICL1 is induced by growth on ethanol and repressed by growth on glucose Gene:ISY1(YJR050W)|FD-Score:4.19|P-value:1.39E-5||SGD DESC:Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles Gene:MCM16(YPR046W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3 Gene:MDL2(YPL270W)|FD-Score:6.42|P-value:6.61E-11||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, required for respiratory growth at high temperature; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis Gene:MDR1(YGR100W)|FD-Score:3.09|P-value:9.96E-4||SGD DESC:Cytoplasmic GTPase-activating protein for Ypt/Rab transport GTPases Ypt6p, Ypt31p and Sec4p; involved in recycling of internalized proteins and regulation of Golgi secretory function Gene:MET12(YPL023C)|FD-Score:3.1|P-value:9.78E-4||SGD DESC:Protein with methylenetetrahydrofolate reductase (MTHFR) activity in vitro; null mutant has no phenotype and is prototrophic for methionine; MET13 encodes major isozyme of MTHFR Gene:MRP7(YNL005C)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NAM7(YMR080C)|FD-Score:3.19|P-value:7.18E-4||SGD DESC:ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress Gene:OPI8(YKR035C_d)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A Gene:PDR3(YBL005W)|FD-Score:4.61|P-value:2.02E-6||SGD DESC:Transcriptional activator of the pleiotropic drug resistance network; regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting sites known as PDREs (PDR responsive elements); post-translationally up-regulated in cells lacking a functional mitochondrial genome; PDR3 has a paralog, PDR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:PFK26(YIL107C)|FD-Score:-3.16|P-value:7.95E-4||SGD DESC:6-phosphofructo-2-kinase, inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate; has negligible fructose-2,6-bisphosphatase activity; transcriptional regulation involves protein kinase A Gene:PKH3(YDR466W)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PUN1(YLR414C)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:Plasma membrane protein with a role in cell wall integrity; co-localizes with Sur7p in punctate membrane patches; null mutant displays decreased thermotolerance; transcription induced upon cell wall damage and metal ion stress Gene:RPL38(YLR325C)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Ribosomal 60S subunit protein L38; homologous to mammalian ribosomal protein L38, no bacterial homolog Gene:RTK1(YDL025C)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress Gene:SCS3(YGL126W)|FD-Score:3.8|P-value:7.31E-5||SGD DESC:Protein required for inositol prototrophy; required for normal ER membrane biosynthesis; ortholog of the FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; disputed role in the synthesis of inositol phospholipids from inositol Gene:SFA1(YDL168W)|FD-Score:5.21|P-value:9.20E-8||SGD DESC:Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress Gene:SSA1(YAL005C)|FD-Score:4|P-value:3.14E-5||SGD DESC:ATPase involved in protein folding and NLS-directed nuclear transport; member of HSP70 family; forms chaperone complex with Ydj1p; localized to nucleus, cytoplasm, and cell wall; 98% identical with paralog Ssa2p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes; general targeting factor of Hsp104p to prion fibrils Gene:SWT21(YNL187W)|FD-Score:-3.26|P-value:5.56E-4||SGD DESC:Protein involved in mRNA splicing; contains a consensus nuclear export signal (NES) sequence similar to the consensus sequence recognized by Crm1p; interacts genetically with Prp40p and Tgs1p; contains WD40 repeats Gene:TAX4(YJL083W)|FD-Score:-3.54|P-value:1.98E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; TAX4 has a paralog, IRS4, that arose from the whole genome duplication Gene:TMS1(YDR105C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Vacuolar membrane protein of unknown function that is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance Gene:UBC7(YMR022W)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly Gene:YGL039W(YGL039W)|FD-Score:-3.22|P-value:6.35E-4||SGD DESC:Oxidoreductase shown to reduce carbonyl compounds to chiral alcohols Gene:YGR149W(YGR149W_p)|FD-Score:-3.32|P-value:4.51E-4||SGD DESC:Putative protein of unknown function; predicted to be an integal membrane protein Gene:YMR074C(YMR074C)|FD-Score:-3.17|P-value:7.50E-4||SGD DESC:Protein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YMR122C(YMR122C_d)|FD-Score:3.79|P-value:7.56E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR141C(YMR141C_d)|FD-Score:3.25|P-value:5.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL057W(YOL057W)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Dipeptidyl-peptidase III; cleaves dipeptides from the amino terminus of target proteins; highly active on synthetic substrate Arg-Arg-2-naphthylamide; mammalian ortholog may be a biomarker for some cancers Gene:YOR034C-A(YOR034C-A_p)|FD-Score:-4.01|P-value:3.00E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YPK2(YMR104C)|FD-Score:-3.24|P-value:5.90E-4||SGD DESC:Protein kinase with similarity to serine/threonine protein kinase Ypk1p; functionally redundant with YPK1 at the genetic level; participates in a signaling pathway required for optimal cell wall integrity; homolog of mammalian kinase SGK; YPK2 has a paralog, YPK1, that arose from the whole genome duplication Gene:YPR050C(YPR050C_d)|FD-Score:4.15|P-value:1.63E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR176W3.768.64E-50.36HEM15Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway
YPR085C3.492.43E-40.36ASA1Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1
YPL228W3.452.76E-40.36CET1Beta (RNA 5'-triphosphatase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CEG1, a guanylyltransferase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide
YJR093C3.099.99E-40.08FIP1Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p
YKL172W3.010.001290.02EBP2Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering
YLR033W2.990.001380.04RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YDL043C2.950.001590.05PRP11Subunit of the SF3a splicing factor complex, required for spliceosome assembly
YIL031W2.900.001840.04ULP2Peptidase that deconjugates Smt3/SUMO-1 peptides from proteins, plays a role in chromosome cohesion at centromeric regions and recovery from checkpoint arrest induced by DNA damage or DNA replication defects; potential Cdc28p substrate
YDR189W2.860.002090.03SLY1Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex
YFR037C2.840.002280.06RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YKL045W2.780.002740.02PRI2Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
YLR076C_d2.760.002920.11YLR076C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10
YLR153C2.640.004100.05ACS2Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions
YLR175W2.600.004720.00CBF5Pseudouridine synthase catalytic subunit of box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs), acts on both large and small rRNAs and on snRNA U2; mutations in human ortholog dyskerin cause the disorder dyskeratosis congenita
YNL062C2.590.004740.03GCD10Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPL270W6.426.61E-11MDL2Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, required for respiratory growth at high temperature; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis
YDL168W5.219.20E-8SFA1Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress
YBL005W4.612.02E-6PDR3Transcriptional activator of the pleiotropic drug resistance network; regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting sites known as PDREs (PDR responsive elements); post-translationally up-regulated in cells lacking a functional mitochondrial genome; PDR3 has a paralog, PDR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YJR050W4.191.39E-5ISY1Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles
YPR050C_d4.151.63E-5YPR050C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W
YAL005C4.003.14E-5SSA1ATPase involved in protein folding and NLS-directed nuclear transport; member of HSP70 family; forms chaperone complex with Ydj1p; localized to nucleus, cytoplasm, and cell wall; 98% identical with paralog Ssa2p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes; general targeting factor of Hsp104p to prion fibrils
YGL126W3.807.31E-5SCS3Protein required for inositol prototrophy; required for normal ER membrane biosynthesis; ortholog of the FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; disputed role in the synthesis of inositol phospholipids from inositol
YMR122C_d3.797.56E-5YMR122C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YNL005C3.681.19E-4MRP7Mitochondrial ribosomal protein of the large subunit
YLR077W3.621.46E-4FMP25Mitochondrial inner membrane protein required for an early step in assembly of respiratory complex III (cytochrome bc1 complex); mRNA is targeted to mitochondria
YDR105C3.611.51E-4TMS1Vacuolar membrane protein of unknown function that is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance
YPR046W3.522.14E-4MCM16Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3
YDL025C3.512.22E-4RTK1Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress
YKR035C_d3.512.23E-4OPI8_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A
YDR466W3.432.99E-4PKH3Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant

GO enrichment analysis for SGTC_3005
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1211.44E-20SGTC_29929074591 71.4 μMChembridge (Drug-like library)177413210.15493
0.1171.76E-19SGTC_29909074105 71.4 μMChembridge (Drug-like library)65020010.0657895
0.1172.48E-19SGTC_23649055074 200.0 μMChembridge (Fragment library)64706300.136364
0.1102.17E-17SGTC_29619083290 32.5 μMChembridge (Drug-like library)177414900.107692
0.0898.50E-12SGTC_20315180455 200.0 μMChembridge (Fragment library)299490.0425532
0.0864.28E-11SGTC_29569076196 2.5 μMChembridge (Drug-like library)164238680.176471
0.0854.75E-11SGTC_30089078912 71.4 μMChembridge (Drug-like library)166487310.288136
0.0832.00E-10SGTC_484loperamide 105.0 μMMiscellaneous39550.133333
0.0723.06E-8SGTC_2039Aminomethylbenzodioxan 200.0 μMChembridge (Fragment library)150530.0689655
0.0691.26E-7SGTC_6811642-0006 26.6 μMChemDiv (Drug-like library)44465300.208955
0.0691.27E-7SGTC_2630antimycin a 100.0 μMMicrosource (Natural product library)57021990.105263mitochondrial processes
0.0681.52E-7SGTC_1905st060207 84.5 μMTimTec (Natural product derivative library)19485470.121212
0.0672.61E-7SGTC_10462001-0062 216.0 μMChemDiv (Drug-like library)7397780.105263
0.0672.84E-7SGTC_60844-0013 19.8 μMChemDiv (Drug-like library)15849000.0428571
0.0655.63E-7SGTC_479n-linoleoylglycine 10.0 μMICCB bioactive library64333460.0757576

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_3009908081971.43 μM0.47169817027216Chembridge (Drug-like library)342.388982.43115
SGTC_2877904072471.43 μM0.450986463895Chembridge (Drug-like library)285.337663.30813
SGTC_3077911731949.47 μM0.4509817154678Chembridge (Drug-like library)285.337663.30813
SGTC_3219913111249.47 μM0.43137317612988Chembridge (Drug-like library)298.3546633.4513Golgi
SGTC_2999906976571.43 μM0.4230772097066Chembridge (Drug-like library)285.337663.30813amide catabolism
SGTC_3103912082249.47 μM0.40983617141819Chembridge (Drug-like library)327.374342.9414
SGTC_9383126-1755342 μM0.4038461777771ChemDiv (Drug-like library)390.388723.01235
SGTC_3067911743149.47 μM0.40384617150885Chembridge (Drug-like library)285.337663.30813
SGTC_29087930301100 μM0.42223178Chembridge (Drug-like library)387.431143.00533
SGTC_3013908013371.43 μM0.417050193Chembridge (Drug-like library)354.442743.64214