9080845

N-(pyridin-4-ylmethyl)-3-(4-pyrrol-1-ylphenyl)-1,2,4-oxadiazole-5-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3015
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 5296973
SMILES C1=CN(C=C1)C2=CC=C(C=C2)C3=NOC(=N3)C(=O)NCC4=CC=NC=C4
Standardized SMILES O=C(NCc1ccncc1)c2onc(n2)c3ccc(cc3)n4cccc4
Molecular weight 345.3547
ALogP 2.14
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.57
% growth inhibition (Hom. pool) 0


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5296973
Download HIP data (tab-delimited text)  (excel)
Gene:CDC13(YDL220C)|FD-Score:3.66|P-value:1.27E-4|Clearance:0.29||SGD DESC:Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation Gene:CDC42(YLR229C)|FD-Score:3.37|P-value:3.73E-4|Clearance:0.17||SGD DESC:Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins Gene:FCP1(YMR277W)|FD-Score:6.23|P-value:2.36E-10|Clearance:1.31||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:KEI1(YDR367W)|FD-Score:-3.92|P-value:4.39E-5|Clearance:0||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:PCF11(YDR228C)|FD-Score:-3.81|P-value:7.06E-5|Clearance:0||SGD DESC:mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping Gene:RPN11(YFR004W)|FD-Score:4.92|P-value:4.40E-7|Clearance:0.72||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:SEC7(YDR170C)|FD-Score:-3.11|P-value:9.25E-4|Clearance:0||SGD DESC:Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles Gene:SPC19(YDR201W)|FD-Score:3.2|P-value:6.81E-4|Clearance:0.22||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body Gene:STT4(YLR305C)|FD-Score:4.19|P-value:1.37E-5|Clearance:0.54||SGD DESC:Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization Gene:CDC13(YDL220C)|FD-Score:3.66|P-value:1.27E-4|Clearance:0.29||SGD DESC:Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation Gene:CDC42(YLR229C)|FD-Score:3.37|P-value:3.73E-4|Clearance:0.17||SGD DESC:Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins Gene:FCP1(YMR277W)|FD-Score:6.23|P-value:2.36E-10|Clearance:1.31||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:KEI1(YDR367W)|FD-Score:-3.92|P-value:4.39E-5|Clearance:0||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:PCF11(YDR228C)|FD-Score:-3.81|P-value:7.06E-5|Clearance:0||SGD DESC:mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping Gene:RPN11(YFR004W)|FD-Score:4.92|P-value:4.40E-7|Clearance:0.72||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:SEC7(YDR170C)|FD-Score:-3.11|P-value:9.25E-4|Clearance:0||SGD DESC:Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles Gene:SPC19(YDR201W)|FD-Score:3.2|P-value:6.81E-4|Clearance:0.22||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body Gene:STT4(YLR305C)|FD-Score:4.19|P-value:1.37E-5|Clearance:0.54||SGD DESC:Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5296973
Download HOP data (tab-delimited text)  (excel)
Gene:ALG8(YOR067C)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p Gene:AMS1(YGL156W)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Vacuolar alpha mannosidase, involved in free oligosaccharide (fOS) degradation; delivered to the vacuole in a novel pathway separate from the secretory pathway Gene:APM4(YOL062C)|FD-Score:3.4|P-value:3.31E-4||SGD DESC:Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport Gene:ASN1(YPR145W)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication Gene:ATP25(YMR098C)|FD-Score:-3.19|P-value:7.22E-4||SGD DESC:Mitochondrial protein required for the stability of Oli1p (Atp9p) mRNA and for the Oli1p ring formation; YMR098C is not an essential gene Gene:CAC2(YML102W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Subunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1p; chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:CGI121(YML036W)|FD-Score:3.73|P-value:9.71E-5||SGD DESC:Component of the EKC/KEOPS complex with Bud32p, Kae1p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription; Cgi121p is dispensable for tRNA modification Gene:COX10(YPL172C)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders Gene:ENT3(YJR125C)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p Gene:GLN3(YER040W)|FD-Score:-3.73|P-value:9.68E-5||SGD DESC:Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source Gene:KNH1(YDL049C)|FD-Score:3.29|P-value:4.98E-4||SGD DESC:Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance Gene:LDB7(YBL006C)|FD-Score:4.65|P-value:1.63E-6||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:LYS4(YDR234W)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway Gene:MLP2(YIL149C)|FD-Score:-3.12|P-value:8.96E-4||SGD DESC:Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length Gene:NAT4(YMR069W)|FD-Score:-3.2|P-value:6.75E-4||SGD DESC:N alpha-acetyl-transferase, involved in acetylation of the N-terminal residues of histones H4 and H2A Gene:NCE102(YPR149W)|FD-Score:-3.26|P-value:5.56E-4||SGD DESC:Protein of unknown function; contains transmembrane domains; involved in secretion of proteins that lack classical secretory signal sequences; component of the detergent-insoluble glycolipid-enriched complexes (DIGs) Gene:NIS1(YNL078W)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Protein localized in the bud neck at G2/M phase; physically interacts with septins; possibly involved in a mitotic signaling network Gene:NQM1(YGR043C)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Transaldolase of unknown function; transcription is repressed by Mot1p and induced by alpha-factor and during diauxic shift Gene:NVJ1(YHR195W)|FD-Score:-3.26|P-value:5.59E-4||SGD DESC:Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) Gene:PHO3(YBR092C)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Constitutively expressed acid phosphatase similar to Pho5p; brought to the cell surface by transport vesicles; hydrolyzes thiamin phosphates in the periplasmic space, increasing cellular thiamin uptake; expression is repressed by thiamin Gene:QCR8(YJL166W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p Gene:RPL26B(YGR034W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication Gene:RPL43A(YPR043W)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Ribosomal 60S subunit protein L43A; null mutation confers a dominant lethal phenotype; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43A has a paralog, RPL43B, that arose from the whole genome duplication Gene:SAM1(YLR180W)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM1 has a paralog, SAM2, that arose from the whole genome duplication Gene:SAP190(YKR028W)|FD-Score:3.13|P-value:8.65E-4||SGD DESC:Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p; SAP190 has a paralog, SAP185, that arose from the whole genome duplication Gene:SCS3(YGL126W)|FD-Score:5.27|P-value:6.70E-8||SGD DESC:Protein required for inositol prototrophy; required for normal ER membrane biosynthesis; ortholog of the FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; disputed role in the synthesis of inositol phospholipids from inositol Gene:SEC22(YLR268W)|FD-Score:3.94|P-value:4.05E-5||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SLX5(YDL013W)|FD-Score:-3.18|P-value:7.33E-4||SGD DESC:Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers Gene:VID22(YLR373C)|FD-Score:-3.77|P-value:8.29E-5||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:WHI3(YNL197C)|FD-Score:-3.37|P-value:3.73E-4||SGD DESC:RNA binding protein that sequesters CLN3 mRNA in cytoplasmic foci;; Tpk1 (PKA) mediated phosphorylation of Whi3 affects G1/S transition by modulating Cln3 transcription; cytoplasmic retention factor for Cdc28p and associated cyclins; regulates cell fate and dose-dependently regulates the critical cell size required for passage through Start; WHI3 has a paralog, WHI4, that arose from the whole genome duplication Gene:YBL012C(YBL012C_d)|FD-Score:3.2|P-value:6.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL086W(YDL086W_p)|FD-Score:3.13|P-value:8.75E-4||SGD DESC:Putative carboxymethylenebutenolidase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL086W is not an essential gene Gene:YKL023W(YKL023W_p)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:Putative protein of unknown function, predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YLR152C(YLR152C_p)|FD-Score:4.03|P-value:2.80E-5||SGD DESC:Putative protein of unknown function; YLR152C is not an essential gene Gene:YLR241W(YLR241W_p)|FD-Score:4.18|P-value:1.43E-5||SGD DESC:Putative protein of unknown function, may be involved in detoxification Gene:YMR082C(YMR082C_d)|FD-Score:4.29|P-value:8.81E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR090W(YMR090W_p)|FD-Score:-4.34|P-value:7.01E-6||SGD DESC:Putative protein of unknown function with similarity to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability Gene:YMR244W(YMR244W_p)|FD-Score:-3.11|P-value:9.48E-4||SGD DESC:Putative protein of unknown function Gene:YPL150W(YPL150W_p)|FD-Score:4.41|P-value:5.20E-6||SGD DESC:Protein kinase of unknown cellular role; binds phosphatidylinositols and cardiolipin in a large-scale study Gene:YPR015C(YPR015C_p)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YPR078C(YPR078C_p)|FD-Score:4.86|P-value:5.78E-7||SGD DESC:Putative protein of unknown function; possible role in DNA metabolism and/or in genome stability; expression is heat-inducible Gene:YTA12(YMR089C)|FD-Score:-3.84|P-value:6.19E-5||SGD DESC:Component, with Afg3p, of the mitochondrial inner membrane m-AAA protease that mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes Gene:ALG8(YOR067C)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p Gene:AMS1(YGL156W)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Vacuolar alpha mannosidase, involved in free oligosaccharide (fOS) degradation; delivered to the vacuole in a novel pathway separate from the secretory pathway Gene:APM4(YOL062C)|FD-Score:3.4|P-value:3.31E-4||SGD DESC:Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport Gene:ASN1(YPR145W)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication Gene:ATP25(YMR098C)|FD-Score:-3.19|P-value:7.22E-4||SGD DESC:Mitochondrial protein required for the stability of Oli1p (Atp9p) mRNA and for the Oli1p ring formation; YMR098C is not an essential gene Gene:CAC2(YML102W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Subunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1p; chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:CGI121(YML036W)|FD-Score:3.73|P-value:9.71E-5||SGD DESC:Component of the EKC/KEOPS complex with Bud32p, Kae1p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription; Cgi121p is dispensable for tRNA modification Gene:COX10(YPL172C)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders Gene:ENT3(YJR125C)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p Gene:GLN3(YER040W)|FD-Score:-3.73|P-value:9.68E-5||SGD DESC:Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source Gene:KNH1(YDL049C)|FD-Score:3.29|P-value:4.98E-4||SGD DESC:Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance Gene:LDB7(YBL006C)|FD-Score:4.65|P-value:1.63E-6||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:LYS4(YDR234W)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway Gene:MLP2(YIL149C)|FD-Score:-3.12|P-value:8.96E-4||SGD DESC:Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length Gene:NAT4(YMR069W)|FD-Score:-3.2|P-value:6.75E-4||SGD DESC:N alpha-acetyl-transferase, involved in acetylation of the N-terminal residues of histones H4 and H2A Gene:NCE102(YPR149W)|FD-Score:-3.26|P-value:5.56E-4||SGD DESC:Protein of unknown function; contains transmembrane domains; involved in secretion of proteins that lack classical secretory signal sequences; component of the detergent-insoluble glycolipid-enriched complexes (DIGs) Gene:NIS1(YNL078W)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Protein localized in the bud neck at G2/M phase; physically interacts with septins; possibly involved in a mitotic signaling network Gene:NQM1(YGR043C)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Transaldolase of unknown function; transcription is repressed by Mot1p and induced by alpha-factor and during diauxic shift Gene:NVJ1(YHR195W)|FD-Score:-3.26|P-value:5.59E-4||SGD DESC:Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) Gene:PHO3(YBR092C)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Constitutively expressed acid phosphatase similar to Pho5p; brought to the cell surface by transport vesicles; hydrolyzes thiamin phosphates in the periplasmic space, increasing cellular thiamin uptake; expression is repressed by thiamin Gene:QCR8(YJL166W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p Gene:RPL26B(YGR034W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication Gene:RPL43A(YPR043W)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Ribosomal 60S subunit protein L43A; null mutation confers a dominant lethal phenotype; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43A has a paralog, RPL43B, that arose from the whole genome duplication Gene:SAM1(YLR180W)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM1 has a paralog, SAM2, that arose from the whole genome duplication Gene:SAP190(YKR028W)|FD-Score:3.13|P-value:8.65E-4||SGD DESC:Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p; SAP190 has a paralog, SAP185, that arose from the whole genome duplication Gene:SCS3(YGL126W)|FD-Score:5.27|P-value:6.70E-8||SGD DESC:Protein required for inositol prototrophy; required for normal ER membrane biosynthesis; ortholog of the FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; disputed role in the synthesis of inositol phospholipids from inositol Gene:SEC22(YLR268W)|FD-Score:3.94|P-value:4.05E-5||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SLX5(YDL013W)|FD-Score:-3.18|P-value:7.33E-4||SGD DESC:Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers Gene:VID22(YLR373C)|FD-Score:-3.77|P-value:8.29E-5||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:WHI3(YNL197C)|FD-Score:-3.37|P-value:3.73E-4||SGD DESC:RNA binding protein that sequesters CLN3 mRNA in cytoplasmic foci;; Tpk1 (PKA) mediated phosphorylation of Whi3 affects G1/S transition by modulating Cln3 transcription; cytoplasmic retention factor for Cdc28p and associated cyclins; regulates cell fate and dose-dependently regulates the critical cell size required for passage through Start; WHI3 has a paralog, WHI4, that arose from the whole genome duplication Gene:YBL012C(YBL012C_d)|FD-Score:3.2|P-value:6.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL086W(YDL086W_p)|FD-Score:3.13|P-value:8.75E-4||SGD DESC:Putative carboxymethylenebutenolidase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL086W is not an essential gene Gene:YKL023W(YKL023W_p)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:Putative protein of unknown function, predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YLR152C(YLR152C_p)|FD-Score:4.03|P-value:2.80E-5||SGD DESC:Putative protein of unknown function; YLR152C is not an essential gene Gene:YLR241W(YLR241W_p)|FD-Score:4.18|P-value:1.43E-5||SGD DESC:Putative protein of unknown function, may be involved in detoxification Gene:YMR082C(YMR082C_d)|FD-Score:4.29|P-value:8.81E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR090W(YMR090W_p)|FD-Score:-4.34|P-value:7.01E-6||SGD DESC:Putative protein of unknown function with similarity to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability Gene:YMR244W(YMR244W_p)|FD-Score:-3.11|P-value:9.48E-4||SGD DESC:Putative protein of unknown function Gene:YPL150W(YPL150W_p)|FD-Score:4.41|P-value:5.20E-6||SGD DESC:Protein kinase of unknown cellular role; binds phosphatidylinositols and cardiolipin in a large-scale study Gene:YPR015C(YPR015C_p)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YPR078C(YPR078C_p)|FD-Score:4.86|P-value:5.78E-7||SGD DESC:Putative protein of unknown function; possible role in DNA metabolism and/or in genome stability; expression is heat-inducible Gene:YTA12(YMR089C)|FD-Score:-3.84|P-value:6.19E-5||SGD DESC:Component, with Afg3p, of the mitochondrial inner membrane m-AAA protease that mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YMR277W6.232.36E-101.31FCP1Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p)
YFR004W4.924.40E-70.72RPN11Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress
YLR305C4.191.37E-50.54STT4Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization
YDL220C3.661.27E-40.28CDC13Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation
YLR229C3.373.73E-40.17CDC42Small rho-like GTPase, essential for establishment and maintenance of cell polarity; mutants have defects in the organization of actin and septins
YDR201W3.206.81E-40.22SPC19Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body
YBR193C2.980.001440.10MED8Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YMR033W2.870.002020.04ARP9Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YOL135C2.830.002310.02MED7Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YGL120C2.810.002470.01PRP43RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome
YDR412W2.800.002580.02RRP17Component of the pre-60S pre-ribosomal particle; required for cell viability under standard (aerobic) conditions but not under anaerobic conditions; exonuclease required for 5′ end processing of pre-60S ribosomal RNA
YKR025W2.780.002730.04RPC37RNA polymerase III subunit C37
YNL103W2.730.003120.09MET4Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p
YBR089W_d2.640.004120.02YBR089W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene POL30
YDR361C2.620.004330.03BCP1Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL126W5.276.70E-8SCS3Protein required for inositol prototrophy; required for normal ER membrane biosynthesis; ortholog of the FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; disputed role in the synthesis of inositol phospholipids from inositol
YPR078C_p4.865.78E-7YPR078C_pPutative protein of unknown function; possible role in DNA metabolism and/or in genome stability; expression is heat-inducible
YBL006C4.651.63E-6LDB7Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions
YPL150W_p4.415.20E-6YPL150W_pProtein kinase of unknown cellular role; binds phosphatidylinositols and cardiolipin in a large-scale study
YMR082C_d4.298.81E-6YMR082C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YLR241W_p4.181.43E-5YLR241W_pPutative protein of unknown function, may be involved in detoxification
YLR152C_p4.032.80E-5YLR152C_pPutative protein of unknown function; YLR152C is not an essential gene
YLR268W3.944.05E-5SEC22R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog
YML036W3.739.71E-5CGI121Component of the EKC/KEOPS complex with Bud32p, Kae1p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription; Cgi121p is dispensable for tRNA modification
YPR043W3.711.02E-4RPL43ARibosomal 60S subunit protein L43A; null mutation confers a dominant lethal phenotype; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43A has a paralog, RPL43B, that arose from the whole genome duplication
YJL166W3.701.09E-4QCR8Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
YGR043C3.591.66E-4NQM1Transaldolase of unknown function; transcription is repressed by Mot1p and induced by alpha-factor and during diauxic shift
YML102W3.561.85E-4CAC2Subunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1p; chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription
YGL156W3.561.88E-4AMS1Vacuolar alpha mannosidase, involved in free oligosaccharide (fOS) degradation; delivered to the vacuole in a novel pathway separate from the secretory pathway
YGR034W3.532.05E-4RPL26BRibosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication

GO enrichment analysis for SGTC_3015
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0791.21E-9SGTC_14394092-1168 46.0 μMChemDiv (Drug-like library)28862400.0740741
0.0706.27E-8SGTC_14123909-8563 178.0 μMChemDiv (Drug-like library)237675300.113402
0.0663.45E-7SGTC_8630466-0191 129.0 μMChemDiv (Drug-like library)7651600.0657895cell wall
0.0579.97E-6SGTC_33569151811 4.5 μMChembridge (Drug-like library)18592540.166667cell wall
0.0542.88E-5SGTC_25422-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene 100.0 μMMicrosource (Natural product library)50247450.0135135
0.0535.07E-5SGTC_1753st043925 64.0 μMTimTec (Natural product derivative library)6701810.0853659
0.0526.27E-5SGTC_23489022543 33.4 μMChembridge (Fragment library)31626780.08mitochondrial processes
0.0526.95E-5SGTC_1851st056236 18.9 μMTimTec (Natural product derivative library)53931710.0641026
0.0518.30E-5SGTC_31419094609 49.5 μMChembridge (Drug-like library)252363090.308824iron homeostasis
0.0501.05E-4SGTC_31349127454 49.5 μMChembridge (Drug-like library)242628200.0958904
0.0501.21E-4SGTC_2416tofa 880.0 nMMiscellaneous1151750.112676sphingolipid biosynthesis & PDR1
0.0501.29E-4SGTC_31659101544 49.5 μMChembridge (Drug-like library)50021840.121622
0.0491.55E-4SGTC_30199081971 49.5 μMChembridge (Drug-like library)164518030.109756
0.0482.01E-4SGTC_8490386-0493 241.0 μMChemDiv (Drug-like library)7261190.09375
0.0482.55E-4SGTC_20375169046 200.0 μMChembridge (Fragment library)57180220.097222260S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2997906707571.43 μM0.48275927209819Chembridge (Drug-like library)297.2837832.62315unfolded protein response
SGTC_21535569715200 μM0.3620692054701Chembridge (Fragment library)281.13732.79912iron homeostasis
SGTC_23899091542195.52 μM0.3620693626624Chembridge (Fragment library)252.719961.88313iron homeostasis
SGTC_22917917025104.35 μM0.3384622964612Chembridge (Fragment library)280.321143.03813iron homeostasis
SGTC_3161910038849.47 μM0.32835825236368Chembridge (Drug-like library)381.67474.03713iron homeostasis
SGTC_23649055074200 μM0.31256470630Chembridge (Fragment library)275.733462.52922
SGTC_3141909460949.47 μM0.30882425236309Chembridge (Drug-like library)381.67474.03713iron homeostasis
SGTC_1916st06760649.2 μM0.29761919590279TimTec (Natural product derivative library)406.391422.78427
SGTC_3142908649849.47 μM0.29166725236367Chembridge (Drug-like library)300.41853.51513iron homeostasis
SGTC_22406690706185.95 μM0.275362805876Chembridge (Fragment library)266.294562.55213