9080866

N-[2-methoxy-5-(3-methyl-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazol-6-yl)phenyl]butanamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3016
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 17066299
SMILES CCCC(=O)NC1=C(C=CC(=C1)C2=NN3C(=NN=C3S2)C)OC
Standardized SMILES CCCC(=O)Nc1cc(ccc1OC)c2nn3c(C)nnc3s2
Molecular weight 331.3928
ALogP 1.94
H-bond donor count 1
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.65
% growth inhibition (Hom. pool) -1.27


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 17066299
Download HIP data (tab-delimited text)  (excel)
Gene:ARP9(YMR033W)|FD-Score:3.53|P-value:2.09E-4|Clearance:0.26||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BBP1(YPL255W)|FD-Score:-3.62|P-value:1.47E-4|Clearance:0||SGD DESC:Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periphery; forms a complex with a nuclear envelope protein Mps2p and SPB components Spc29p and Kar1p; required for mitotic functions of Cdc5p Gene:BET1(YIL004C)|FD-Score:3.49|P-value:2.40E-4|Clearance:0.26||SGD DESC:Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins Gene:CIA1(YDR267C)|FD-Score:3.62|P-value:1.45E-4|Clearance:0.26||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia2p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; contains WD40 repeats; ortholog of human Ciao1 protein Gene:GRS1(YBR121C)|FD-Score:3.99|P-value:3.37E-5|Clearance:0.26||SGD DESC:Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication Gene:KAP95(YLR347C)|FD-Score:3.38|P-value:3.57E-4|Clearance:0.26||SGD DESC:Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p Gene:MET4(YNL103W)|FD-Score:-3.44|P-value:2.87E-4|Clearance:0||SGD DESC:Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p Gene:PGS1(YCL004W)|FD-Score:4.23|P-value:1.15E-5|Clearance:0.26||SGD DESC:Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis Gene:PRP22(YER013W)|FD-Score:3.32|P-value:4.42E-4|Clearance:0.26||SGD DESC:DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes Gene:RPF1(YHR088W)|FD-Score:3.84|P-value:6.24E-5|Clearance:0.26||SGD DESC:Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA Gene:RPL17A(YKL180W)|FD-Score:3.35|P-value:4.01E-4|Clearance:0.26||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPP0(YLR340W)|FD-Score:4.08|P-value:2.26E-5|Clearance:0.26||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:RSC3(YDR303C)|FD-Score:-4.15|P-value:1.66E-5|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for maintenance of proper ploidy and regulation of ribosomal protein genes and the cell wall/stress response; highly similar to Rsc30p Gene:SCM3(YDL139C)|FD-Score:-6.14|P-value:4.14E-10|Clearance:0||SGD DESC:Nonhistone component of centromeric chromatin; binds stoichiometrically to CenH3-H4 histones, required for kinetochore assembly; required for G2/M progression and localization of Cse4p; may protect Cse4p from ubiquitylation; homolog of mammalian HJURP Gene:TUB2(YFL037W)|FD-Score:-3.86|P-value:5.57E-5|Clearance:0||SGD DESC:Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria Gene:ARP9(YMR033W)|FD-Score:3.53|P-value:2.09E-4|Clearance:0.26||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BBP1(YPL255W)|FD-Score:-3.62|P-value:1.47E-4|Clearance:0||SGD DESC:Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periphery; forms a complex with a nuclear envelope protein Mps2p and SPB components Spc29p and Kar1p; required for mitotic functions of Cdc5p Gene:BET1(YIL004C)|FD-Score:3.49|P-value:2.40E-4|Clearance:0.26||SGD DESC:Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins Gene:CIA1(YDR267C)|FD-Score:3.62|P-value:1.45E-4|Clearance:0.26||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia2p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; contains WD40 repeats; ortholog of human Ciao1 protein Gene:GRS1(YBR121C)|FD-Score:3.99|P-value:3.37E-5|Clearance:0.26||SGD DESC:Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication Gene:KAP95(YLR347C)|FD-Score:3.38|P-value:3.57E-4|Clearance:0.26||SGD DESC:Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p Gene:MET4(YNL103W)|FD-Score:-3.44|P-value:2.87E-4|Clearance:0||SGD DESC:Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p Gene:PGS1(YCL004W)|FD-Score:4.23|P-value:1.15E-5|Clearance:0.26||SGD DESC:Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis Gene:PRP22(YER013W)|FD-Score:3.32|P-value:4.42E-4|Clearance:0.26||SGD DESC:DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes Gene:RPF1(YHR088W)|FD-Score:3.84|P-value:6.24E-5|Clearance:0.26||SGD DESC:Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA Gene:RPL17A(YKL180W)|FD-Score:3.35|P-value:4.01E-4|Clearance:0.26||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPP0(YLR340W)|FD-Score:4.08|P-value:2.26E-5|Clearance:0.26||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:RSC3(YDR303C)|FD-Score:-4.15|P-value:1.66E-5|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for maintenance of proper ploidy and regulation of ribosomal protein genes and the cell wall/stress response; highly similar to Rsc30p Gene:SCM3(YDL139C)|FD-Score:-6.14|P-value:4.14E-10|Clearance:0||SGD DESC:Nonhistone component of centromeric chromatin; binds stoichiometrically to CenH3-H4 histones, required for kinetochore assembly; required for G2/M progression and localization of Cse4p; may protect Cse4p from ubiquitylation; homolog of mammalian HJURP Gene:TUB2(YFL037W)|FD-Score:-3.86|P-value:5.57E-5|Clearance:0||SGD DESC:Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 17066299
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:8.5|P-value:9.38E-18||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ACO1(YLR304C)|FD-Score:3.75|P-value:8.97E-5||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:AIM20(YIL158W)|FD-Score:5.55|P-value:1.39E-8||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:ARF2(YDL137W)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; ARF2 has a paralog, ARF1, that arose from the whole genome duplication Gene:ARO4(YBR249C)|FD-Score:-3.35|P-value:4.00E-4||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan; relative distribution to the nucleus increases upon DNA replication stress Gene:ATP17(YDR377W)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:ATX1(YNL259C)|FD-Score:-3.39|P-value:3.51E-4||SGD DESC:Cytosolic copper metallochaperone that transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake Gene:BAR1(YIL015W)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Aspartyl protease secreted into the periplasmic space of mating type a cells, helps cells find mating partners, cleaves and inactivates alpha factor allowing cells to recover from alpha-factor-induced cell cycle arrest Gene:BDF2(YDL070W)|FD-Score:4.04|P-value:2.62E-5||SGD DESC:Protein involved in transcription initiation; acts at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf1p; protein abundance increases in response to DNA replication stress Gene:CAR1(YPL111W)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:Arginase, responsible for arginine degradation, expression responds to both induction by arginine and nitrogen catabolite repression; disruption enhances freeze tolerance Gene:CDA2(YLR308W)|FD-Score:-3.16|P-value:7.92E-4||SGD DESC:Chitin deacetylase, together with Cda1p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall Gene:COA6(YMR244C-A_p)|FD-Score:5.03|P-value:2.47E-7||SGD DESC:Protein involved in cytochrome c oxidase assembly; also required for efficient formation of respiratory supercomplexes comprised of Complexes III and IV; localizes to the mitochondrial intermembrane space; has a human homolog; transcription is induced in response to the DNA-damaging agent MMS; protein abundance increases in response to DNA replication stress Gene:CYK3(YDL117W)|FD-Score:4.1|P-value:2.04E-5||SGD DESC:SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants Gene:EDE1(YBL047C)|FD-Score:3.12|P-value:8.99E-4||SGD DESC:Endocytic protein; involved in a network of interactions with other endocytic proteins, binds membranes in a ubiquitin-dependent manner, may also bind ubiquitinated membrane-associated proteins; interacts with Cmk2 and functions upstream of CMK2 in regulating non-apoptotic cell death Gene:ELC1(YPL046C)|FD-Score:3.89|P-value:5.06E-5||SGD DESC:Elongin C, conserved among eukaryotes; forms a complex with Cul3p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; plays a role in global genomic repair Gene:ENO1(YGR254W)|FD-Score:4.42|P-value:4.97E-6||SGD DESC:Enolase I, a phosphopyruvate hydratase; catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is repressed in response to glucose; protein abundance increases in response to DNA replication stress Gene:FAR3(YMR052W)|FD-Score:3.14|P-value:8.47E-4||SGD DESC:Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:FCY1(YPR062W)|FD-Score:4.81|P-value:7.66E-7||SGD DESC:Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) Gene:HHF1(YBR009C)|FD-Score:-3.21|P-value:6.60E-4||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HMO1(YDR174W)|FD-Score:3.88|P-value:5.32E-5||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:HMS1(YOR032C)|FD-Score:3.16|P-value:8.01E-4||SGD DESC:Basic helix-loop-helix (bHLH) protein with similarity to myc-family transcription factors; overexpression confers hyperfilamentous growth and suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant Gene:HUL5(YGL141W)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Multiubiquitin chain assembly factor (E4); proteasome processivity factor that elongates polyUb chains on substrates, opposing Ubp6p, a branched polyubiquitin protease; required for retrograde transport of misfolded proteins during ERAD Gene:IFA38(YBR159W)|FD-Score:-3.18|P-value:7.35E-4||SGD DESC:Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides Gene:LAS21(YJL062W)|FD-Score:3.88|P-value:5.17E-5||SGD DESC:Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity Gene:LDB7(YBL006C)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:LIA1(YJR070C)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress Gene:LRE1(YCL051W)|FD-Score:4.27|P-value:9.58E-6||SGD DESC:Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway; <br>LRE1 has a paralog, HLR1, that arose from the whole genome duplication Gene:MDM12(YOL009C)|FD-Score:3.1|P-value:9.55E-4||SGD DESC:Mitochondrial outer membrane protein, required for transmission of mitochondria to daughter cells; component of the ERMES complex that links the ER to mitochondria; may influence import and assembly of outer membrane beta-barrel proteins Gene:MKC7(YDR144C)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:GPI-anchored aspartyl protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; shares functions with Yap3p and Kex2p Gene:MND2(YIR025W)|FD-Score:4.88|P-value:5.41E-7||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MRPL23(YOR150W)|FD-Score:6.19|P-value:3.09E-10||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MTL1(YGR023W)|FD-Score:3.12|P-value:9.12E-4||SGD DESC:Putative plasma membrane sensor, involved in cell integrity signaling and stress response during glucose starvation and oxidative stress; has structural and functional similarity to Mid2p Gene:OAZ1(YPL052W)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Regulator of ornithine decarboxylase (Spe1p), antizyme that binds to Spe1p to stimulate ubiquitin-independent degradation by the proteasome; binding of polyamines to nascent Oaz1p during translation stimulates +1 ribosomal frameshifting, allowing translation of full-length Oaz1p Gene:OPY2(YPR075C)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress Gene:ORT1(YOR130C)|FD-Score:3.28|P-value:5.10E-4||SGD DESC:Ornithine transporter of the mitochondrial inner membrane, exports ornithine from mitochondria as part of arginine biosynthesis; human ortholog is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome Gene:PFK1(YGR240C)|FD-Score:5.2|P-value:1.01E-7||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PHO87(YCR037C)|FD-Score:3.91|P-value:4.67E-5||SGD DESC:Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication Gene:PMC1(YGL006W)|FD-Score:-3.47|P-value:2.59E-4||SGD DESC:Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activation of calcineurin in the presence of elevated concentrations of calcium; similar to mammalian PMCA1a Gene:PRM8(YGL053W)|FD-Score:5.56|P-value:1.38E-8||SGD DESC:Pheromone-regulated protein with 2 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; forms a complex with Prp9p in the ER; member of DUP240 gene family Gene:PUT1(YLR142W)|FD-Score:-3.5|P-value:2.34E-4||SGD DESC:Proline oxidase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; PUT1 transcription is induced by Put3p in the presence of proline and the absence of a preferred nitrogen source Gene:RAD5(YLR032W)|FD-Score:4.56|P-value:2.51E-6||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RAD7(YJR052W)|FD-Score:-3.45|P-value:2.78E-4||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad16p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RSA1(YPL193W)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:RSC1(YGR056W)|FD-Score:5.15|P-value:1.31E-7||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:SCJ1(YMR214W)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins Gene:SCW11(YGL028C)|FD-Score:4.48|P-value:3.68E-6||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p Gene:SDH1(YKL148C)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Flavoprotein subunit of succinate dehydrogenase; couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; FAD binding to Sdh1p is required for the assembly of the succinate dehydrogenase subunits Gene:SDH2(YLL041C)|FD-Score:-3.17|P-value:7.74E-4||SGD DESC:Iron-sulfur protein subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SLG1(YOR008C)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SLT2(YHR030C)|FD-Score:-5.73|P-value:5.07E-9||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SOL2(YCR073W-A)|FD-Score:6.39|P-value:8.36E-11||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:TAT1(YBR069C)|FD-Score:-3.96|P-value:3.71E-5||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:THI12(YNL332W)|FD-Score:-3.3|P-value:4.79E-4||SGD DESC:Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP); member of a subtelomeric gene family including THI5, THI11, THI12, and THI13 Gene:THI22(YPR121W)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Protein with similarity to hydroxymethylpyrimidine phosphate kinases; member of a gene family with THI20 and THI21; not required for thiamine biosynthesis Gene:TMA10(YLR327C)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:Protein of unknown function that associates with ribosomes; putative homolog of the F1F0-ATPase synthase regulator Stf2p; protein abundance increases in response to DNA replication stress Gene:TRM10(YOL093W)|FD-Score:3.74|P-value:9.25E-5||SGD DESC:tRNA methyltransferase; methylates the N-1 position of guanosine in tRNAs; protein abundance increases in response to DNA replication stress Gene:VID22(YLR373C)|FD-Score:4.52|P-value:3.03E-6||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:VPS63(YLR261C_d)|FD-Score:-3.17|P-value:7.73E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YBL094C(YBL094C_d)|FD-Score:4.55|P-value:2.64E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YBL095W Gene:YBR056W-A(YBR056W-A_p)|FD-Score:3.25|P-value:5.74E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the dubious ORF YBR056C-B Gene:YDR250C(YDR250C_d)|FD-Score:4.21|P-value:1.25E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YFL032W(YFL032W_d)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene Gene:YFL041W-A(YFL041W-A_p)|FD-Score:4.41|P-value:5.16E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YFR020W(YFR020W_p)|FD-Score:-3.81|P-value:6.92E-5||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YGR064W(YGR064W_d)|FD-Score:4.36|P-value:6.42E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YHR045W(YHR045W_p)|FD-Score:3.99|P-value:3.24E-5||SGD DESC:Putative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum Gene:YLR460C(YLR460C_p)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine Gene:YMR31(YFR049W)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to human mitochondrial ribosomal protein MRP-S36 Gene:YNL155W(YNL155W_p)|FD-Score:3.78|P-value:8.00E-5||SGD DESC:Protein of unknown function; contains a DHHC domain and an AN1-type zinc finger; predicted to have thiol-disulfide oxidoreductase active site; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress Gene:YOR223W(YOR223W)|FD-Score:5.08|P-value:1.92E-7||SGD DESC:Subunit of the DSC ubiquitin ligase complex; protein of unknown function that localizes to the ER and vacuole lumen; overexpression affects endocytic protein trafficking; ortholog of fission yeast dsc3 Gene:YPL245W(YPL245W_p)|FD-Score:4.87|P-value:5.48E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm Gene:YPT6(YLR262C)|FD-Score:-3.91|P-value:4.60E-5||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YSC83(YHR017W)|FD-Score:5.28|P-value:6.36E-8||SGD DESC:Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83 Gene:ZAP1(YJL056C)|FD-Score:-3.12|P-value:8.93E-4||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains Gene:ACB1(YGR037C)|FD-Score:8.5|P-value:9.38E-18||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ACO1(YLR304C)|FD-Score:3.75|P-value:8.97E-5||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:AIM20(YIL158W)|FD-Score:5.55|P-value:1.39E-8||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:ARF2(YDL137W)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; ARF2 has a paralog, ARF1, that arose from the whole genome duplication Gene:ARO4(YBR249C)|FD-Score:-3.35|P-value:4.00E-4||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan; relative distribution to the nucleus increases upon DNA replication stress Gene:ATP17(YDR377W)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:ATX1(YNL259C)|FD-Score:-3.39|P-value:3.51E-4||SGD DESC:Cytosolic copper metallochaperone that transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake Gene:BAR1(YIL015W)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Aspartyl protease secreted into the periplasmic space of mating type a cells, helps cells find mating partners, cleaves and inactivates alpha factor allowing cells to recover from alpha-factor-induced cell cycle arrest Gene:BDF2(YDL070W)|FD-Score:4.04|P-value:2.62E-5||SGD DESC:Protein involved in transcription initiation; acts at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf1p; protein abundance increases in response to DNA replication stress Gene:CAR1(YPL111W)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:Arginase, responsible for arginine degradation, expression responds to both induction by arginine and nitrogen catabolite repression; disruption enhances freeze tolerance Gene:CDA2(YLR308W)|FD-Score:-3.16|P-value:7.92E-4||SGD DESC:Chitin deacetylase, together with Cda1p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall Gene:COA6(YMR244C-A_p)|FD-Score:5.03|P-value:2.47E-7||SGD DESC:Protein involved in cytochrome c oxidase assembly; also required for efficient formation of respiratory supercomplexes comprised of Complexes III and IV; localizes to the mitochondrial intermembrane space; has a human homolog; transcription is induced in response to the DNA-damaging agent MMS; protein abundance increases in response to DNA replication stress Gene:CYK3(YDL117W)|FD-Score:4.1|P-value:2.04E-5||SGD DESC:SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants Gene:EDE1(YBL047C)|FD-Score:3.12|P-value:8.99E-4||SGD DESC:Endocytic protein; involved in a network of interactions with other endocytic proteins, binds membranes in a ubiquitin-dependent manner, may also bind ubiquitinated membrane-associated proteins; interacts with Cmk2 and functions upstream of CMK2 in regulating non-apoptotic cell death Gene:ELC1(YPL046C)|FD-Score:3.89|P-value:5.06E-5||SGD DESC:Elongin C, conserved among eukaryotes; forms a complex with Cul3p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; plays a role in global genomic repair Gene:ENO1(YGR254W)|FD-Score:4.42|P-value:4.97E-6||SGD DESC:Enolase I, a phosphopyruvate hydratase; catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is repressed in response to glucose; protein abundance increases in response to DNA replication stress Gene:FAR3(YMR052W)|FD-Score:3.14|P-value:8.47E-4||SGD DESC:Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:FCY1(YPR062W)|FD-Score:4.81|P-value:7.66E-7||SGD DESC:Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) Gene:HHF1(YBR009C)|FD-Score:-3.21|P-value:6.60E-4||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HMO1(YDR174W)|FD-Score:3.88|P-value:5.32E-5||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:HMS1(YOR032C)|FD-Score:3.16|P-value:8.01E-4||SGD DESC:Basic helix-loop-helix (bHLH) protein with similarity to myc-family transcription factors; overexpression confers hyperfilamentous growth and suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant Gene:HUL5(YGL141W)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Multiubiquitin chain assembly factor (E4); proteasome processivity factor that elongates polyUb chains on substrates, opposing Ubp6p, a branched polyubiquitin protease; required for retrograde transport of misfolded proteins during ERAD Gene:IFA38(YBR159W)|FD-Score:-3.18|P-value:7.35E-4||SGD DESC:Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides Gene:LAS21(YJL062W)|FD-Score:3.88|P-value:5.17E-5||SGD DESC:Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity Gene:LDB7(YBL006C)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:LIA1(YJR070C)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress Gene:LRE1(YCL051W)|FD-Score:4.27|P-value:9.58E-6||SGD DESC:Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway; <br>LRE1 has a paralog, HLR1, that arose from the whole genome duplication Gene:MDM12(YOL009C)|FD-Score:3.1|P-value:9.55E-4||SGD DESC:Mitochondrial outer membrane protein, required for transmission of mitochondria to daughter cells; component of the ERMES complex that links the ER to mitochondria; may influence import and assembly of outer membrane beta-barrel proteins Gene:MKC7(YDR144C)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:GPI-anchored aspartyl protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; shares functions with Yap3p and Kex2p Gene:MND2(YIR025W)|FD-Score:4.88|P-value:5.41E-7||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MRPL23(YOR150W)|FD-Score:6.19|P-value:3.09E-10||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MTL1(YGR023W)|FD-Score:3.12|P-value:9.12E-4||SGD DESC:Putative plasma membrane sensor, involved in cell integrity signaling and stress response during glucose starvation and oxidative stress; has structural and functional similarity to Mid2p Gene:OAZ1(YPL052W)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Regulator of ornithine decarboxylase (Spe1p), antizyme that binds to Spe1p to stimulate ubiquitin-independent degradation by the proteasome; binding of polyamines to nascent Oaz1p during translation stimulates +1 ribosomal frameshifting, allowing translation of full-length Oaz1p Gene:OPY2(YPR075C)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress Gene:ORT1(YOR130C)|FD-Score:3.28|P-value:5.10E-4||SGD DESC:Ornithine transporter of the mitochondrial inner membrane, exports ornithine from mitochondria as part of arginine biosynthesis; human ortholog is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome Gene:PFK1(YGR240C)|FD-Score:5.2|P-value:1.01E-7||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PHO87(YCR037C)|FD-Score:3.91|P-value:4.67E-5||SGD DESC:Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication Gene:PMC1(YGL006W)|FD-Score:-3.47|P-value:2.59E-4||SGD DESC:Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activation of calcineurin in the presence of elevated concentrations of calcium; similar to mammalian PMCA1a Gene:PRM8(YGL053W)|FD-Score:5.56|P-value:1.38E-8||SGD DESC:Pheromone-regulated protein with 2 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; forms a complex with Prp9p in the ER; member of DUP240 gene family Gene:PUT1(YLR142W)|FD-Score:-3.5|P-value:2.34E-4||SGD DESC:Proline oxidase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; PUT1 transcription is induced by Put3p in the presence of proline and the absence of a preferred nitrogen source Gene:RAD5(YLR032W)|FD-Score:4.56|P-value:2.51E-6||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RAD7(YJR052W)|FD-Score:-3.45|P-value:2.78E-4||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad16p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RSA1(YPL193W)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:RSC1(YGR056W)|FD-Score:5.15|P-value:1.31E-7||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:SCJ1(YMR214W)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins Gene:SCW11(YGL028C)|FD-Score:4.48|P-value:3.68E-6||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p Gene:SDH1(YKL148C)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Flavoprotein subunit of succinate dehydrogenase; couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; FAD binding to Sdh1p is required for the assembly of the succinate dehydrogenase subunits Gene:SDH2(YLL041C)|FD-Score:-3.17|P-value:7.74E-4||SGD DESC:Iron-sulfur protein subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SLG1(YOR008C)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SLT2(YHR030C)|FD-Score:-5.73|P-value:5.07E-9||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SOL2(YCR073W-A)|FD-Score:6.39|P-value:8.36E-11||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:TAT1(YBR069C)|FD-Score:-3.96|P-value:3.71E-5||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:THI12(YNL332W)|FD-Score:-3.3|P-value:4.79E-4||SGD DESC:Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP); member of a subtelomeric gene family including THI5, THI11, THI12, and THI13 Gene:THI22(YPR121W)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Protein with similarity to hydroxymethylpyrimidine phosphate kinases; member of a gene family with THI20 and THI21; not required for thiamine biosynthesis Gene:TMA10(YLR327C)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:Protein of unknown function that associates with ribosomes; putative homolog of the F1F0-ATPase synthase regulator Stf2p; protein abundance increases in response to DNA replication stress Gene:TRM10(YOL093W)|FD-Score:3.74|P-value:9.25E-5||SGD DESC:tRNA methyltransferase; methylates the N-1 position of guanosine in tRNAs; protein abundance increases in response to DNA replication stress Gene:VID22(YLR373C)|FD-Score:4.52|P-value:3.03E-6||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:VPS63(YLR261C_d)|FD-Score:-3.17|P-value:7.73E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YBL094C(YBL094C_d)|FD-Score:4.55|P-value:2.64E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YBL095W Gene:YBR056W-A(YBR056W-A_p)|FD-Score:3.25|P-value:5.74E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the dubious ORF YBR056C-B Gene:YDR250C(YDR250C_d)|FD-Score:4.21|P-value:1.25E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YFL032W(YFL032W_d)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene Gene:YFL041W-A(YFL041W-A_p)|FD-Score:4.41|P-value:5.16E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YFR020W(YFR020W_p)|FD-Score:-3.81|P-value:6.92E-5||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YGR064W(YGR064W_d)|FD-Score:4.36|P-value:6.42E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YHR045W(YHR045W_p)|FD-Score:3.99|P-value:3.24E-5||SGD DESC:Putative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum Gene:YLR460C(YLR460C_p)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine Gene:YMR31(YFR049W)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to human mitochondrial ribosomal protein MRP-S36 Gene:YNL155W(YNL155W_p)|FD-Score:3.78|P-value:8.00E-5||SGD DESC:Protein of unknown function; contains a DHHC domain and an AN1-type zinc finger; predicted to have thiol-disulfide oxidoreductase active site; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress Gene:YOR223W(YOR223W)|FD-Score:5.08|P-value:1.92E-7||SGD DESC:Subunit of the DSC ubiquitin ligase complex; protein of unknown function that localizes to the ER and vacuole lumen; overexpression affects endocytic protein trafficking; ortholog of fission yeast dsc3 Gene:YPL245W(YPL245W_p)|FD-Score:4.87|P-value:5.48E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm Gene:YPT6(YLR262C)|FD-Score:-3.91|P-value:4.60E-5||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YSC83(YHR017W)|FD-Score:5.28|P-value:6.36E-8||SGD DESC:Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83 Gene:ZAP1(YJL056C)|FD-Score:-3.12|P-value:8.93E-4||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YCL004W4.231.15E-50.26PGS1Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis
YLR340W4.082.26E-50.26RPP0Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10
YBR121C3.993.37E-50.26GRS1Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication
YHR088W3.846.24E-50.26RPF1Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA
YDR267C3.621.45E-40.26CIA1Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia2p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; contains WD40 repeats; ortholog of human Ciao1 protein
YMR033W3.532.09E-40.26ARP9Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YIL004C3.492.40E-40.26BET1Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins
YLR347C3.383.57E-40.26KAP95Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p
YKL180W3.354.01E-40.26RPL17ARibosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
YER013W3.324.42E-40.26PRP22DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes
YKL082C3.060.001090.17RRP14Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family
YER104W2.890.001920.03RTT105Protein with a role in regulation of Ty1 transposition
YLR075W2.860.002140.00RPL10Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects
YOL040C2.850.002170.01RPS15Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19
YAR019C2.850.002210.03CDC15Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGR037C8.509.38E-18ACB1Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress
YCR073W-A6.398.36E-11SOL2Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication
YOR150W6.193.09E-10MRPL23Mitochondrial ribosomal protein of the large subunit
YGL053W5.561.38E-8PRM8Pheromone-regulated protein with 2 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; forms a complex with Prp9p in the ER; member of DUP240 gene family
YIL158W5.551.39E-8AIM20Putative protein of unknown function; overexpression causes a cell cycle delay or arrest; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from nucleus to cytoplasm upon DNA replication stress
YHR017W5.286.36E-8YSC83Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83
YGR240C5.201.01E-7PFK1Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes
YGR056W5.151.31E-7RSC1Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook
YOR223W5.081.92E-7YOR223WSubunit of the DSC ubiquitin ligase complex; protein of unknown function that localizes to the ER and vacuole lumen; overexpression affects endocytic protein trafficking; ortholog of fission yeast dsc3
YMR244C-A_p5.032.47E-7COA6_pProtein involved in cytochrome c oxidase assembly; also required for efficient formation of respiratory supercomplexes comprised of Complexes III and IV; localizes to the mitochondrial intermembrane space; has a human homolog; transcription is induced in response to the DNA-damaging agent MMS; protein abundance increases in response to DNA replication stress
YIR025W4.885.41E-7MND2Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase
YPL245W_p4.875.48E-7YPL245W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm
YPR062W4.817.66E-7FCY1Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU)
YLR032W4.562.51E-6RAD5DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress
YBL094C_d4.552.64E-6YBL094C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YBL095W

GO enrichment analysis for SGTC_3016
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0863.06E-11SGTC_750159-0059 199.0 μMChemDiv (Drug-like library)53772700.142857fatty acid desaturase (OLE1)
0.0791.31E-9SGTC_10064099-6547 255.0 μMChemDiv (Drug-like library)31468330.0864198
0.0781.77E-9SGTC_21925915882 198.5 μMChembridge (Fragment library)28761490.0779221RNA processing & uracil transport
0.0782.27E-9SGTC_2416tofa 880.0 nMMiscellaneous1151750.150685sphingolipid biosynthesis & PDR1
0.0741.55E-8SGTC_22157188065 200.0 μMChembridge (Fragment library)9402170.101449
0.0731.89E-8SGTC_9511171-0579 82.7 μMChemDiv (Drug-like library)16160500.109756cell wall
0.0723.89E-8SGTC_22297354935 190.0 μMChembridge (Fragment library)3181120.0933333RSC complex & mRNA processing
0.0672.59E-7SGTC_662k832-1615 104.0 μMChemDiv (Drug-like library)58784780.108434
0.0648.49E-7SGTC_33229139058 2.1 μMChembridge (Drug-like library)49089260.147727cell wall
0.0631.23E-6SGTC_2248tofa 1.1 μMMiscellaneous1151750.150685sphingolipid biosynthesis & PDR1
0.0612.63E-6SGTC_28379008476 45.5 μMChembridge (Drug-like library)29928050.141176
0.0612.67E-6SGTC_32859112818 20.9 μMChembridge (Drug-like library)170179470.204819cell wall
0.0613.25E-6SGTC_2745rifampicin 24.3 μMMiscellaneous251414280.0656934
0.0596.37E-6SGTC_30749116018 49.5 μMChembridge (Drug-like library)170273030.141176RNA processing & uracil transport
0.0571.20E-5SGTC_7670929-0023 86.0 μMChemDiv (Drug-like library)12045630.113924TRP & mitochondrial translation

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3277913872449.47 μM0.43859615943418Chembridge (Drug-like library)295.158382.46204Golgi
SGTC_3101912053149.47 μM0.39705924824904Chembridge (Drug-like library)288.368043.27705
SGTC_3123912616249.47 μM0.37525239884Chembridge (Drug-like library)367.424883.32107
SGTC_3300912461550.48 μM0.36363624824903Chembridge (Drug-like library)288.368043.07205
SGTC_2831799903619.48 μM0.3478262988270Chembridge (Drug-like library)323.309654.03115
SGTC_3073911497249.47 μM0.33823541905075Chembridge (Drug-like library)272.368643.5750460S ribosome export
SGTC_3107912180849.47 μM0.333333662681Chembridge (Drug-like library)309.345742.6406
SGTC_3132912670649.47 μM0.333333850858Chembridge (Drug-like library)326.3481433.56706
SGTC_3305912702753.83 μM0.31944441905295Chembridge (Drug-like library)316.378142.87506
SGTC_3299912198243.13 μM0.31884117594604Chembridge (Drug-like library)273.35672.22105