9090631

(3-chloro-4-fluoro-1-benzothiophen-2-yl)-(4-methylpiperidin-1-yl)methanone

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3024
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 25236383
SMILES CC1CCN(CC1)C(=O)C2=C(C3=C(C=CC=C3S2)F)Cl
Standardized SMILES CC1CCN(CC1)C(=O)c2sc3cccc(F)c3c2Cl
Molecular weight 311.8021
ALogP 4.44
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.47
% growth inhibition (Hom. pool) 8.72


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 25236383
Download HIP data (tab-delimited text)  (excel)
Gene:CDC33(YOL139C)|FD-Score:4.74|P-value:1.04E-6|Clearance:0.56||SGD DESC:mRNA cap binding protein and translation initiation factor eIF4E; the eIF4E-cap complex is responsible for mediating cap-dependent mRNA translation via interactions with translation initiation factor eIF4G (Tif4631p or Tif4632p); protein abundance increases in response to DNA replication stress; mutants are defective for adhesion and pseudohyphal growth Gene:IDI1(YPL117C)|FD-Score:3.54|P-value:2.01E-4|Clearance:0.56||SGD DESC:Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability Gene:LSM4(YER112W)|FD-Score:3.74|P-value:9.23E-5|Clearance:0.56||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:MEC1(YBR136W)|FD-Score:3.93|P-value:4.33E-5|Clearance:0.56||SGD DESC:Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres Gene:NCP1(YHR042W)|FD-Score:4.5|P-value:3.44E-6|Clearance:0.56||SGD DESC:NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p Gene:QNS1(YHR074W)|FD-Score:4.04|P-value:2.68E-5|Clearance:0.56||SGD DESC:Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide Gene:SUA5(YGL169W)|FD-Score:3.68|P-value:1.16E-4|Clearance:0.56||SGD DESC:Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family Gene:TOM22(YNL131W)|FD-Score:-3.62|P-value:1.49E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:CDC33(YOL139C)|FD-Score:4.74|P-value:1.04E-6|Clearance:0.56||SGD DESC:mRNA cap binding protein and translation initiation factor eIF4E; the eIF4E-cap complex is responsible for mediating cap-dependent mRNA translation via interactions with translation initiation factor eIF4G (Tif4631p or Tif4632p); protein abundance increases in response to DNA replication stress; mutants are defective for adhesion and pseudohyphal growth Gene:IDI1(YPL117C)|FD-Score:3.54|P-value:2.01E-4|Clearance:0.56||SGD DESC:Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability Gene:LSM4(YER112W)|FD-Score:3.74|P-value:9.23E-5|Clearance:0.56||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:MEC1(YBR136W)|FD-Score:3.93|P-value:4.33E-5|Clearance:0.56||SGD DESC:Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres Gene:NCP1(YHR042W)|FD-Score:4.5|P-value:3.44E-6|Clearance:0.56||SGD DESC:NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p Gene:QNS1(YHR074W)|FD-Score:4.04|P-value:2.68E-5|Clearance:0.56||SGD DESC:Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide Gene:SUA5(YGL169W)|FD-Score:3.68|P-value:1.16E-4|Clearance:0.56||SGD DESC:Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family Gene:TOM22(YNL131W)|FD-Score:-3.62|P-value:1.49E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 25236383
Download HOP data (tab-delimited text)  (excel)
Gene:CBF1(YJR060W)|FD-Score:-3.12|P-value:9.18E-4||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:CIN1(YOR349W)|FD-Score:4.65|P-value:1.63E-6||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:CMR1(YDL156W)|FD-Score:3.14|P-value:8.50E-4||SGD DESC:DNA-binding protein with preference for UV-damaged DNA; protein sequence contains three WD domains (WD-40 repeat); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; potential regulatory target of Mbp1p, which binds to the promoter region; co-localizes with Hos2p in nuclear foci in response to DNA damage by MMS Gene:CPA2(YJR109C)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Large subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor Gene:CST26(YBR042C)|FD-Score:6.92|P-value:2.20E-12||SGD DESC:Putative transferase involved in phospholipid biosynthesis; required for incorporation of stearic acid into phosphatidylinositol; affects chromosome stability when overexpressed; CST26 has a paralog, YDR018C, that arose from the whole genome duplication Gene:ERD1(YDR414C)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) Gene:FAU1(YER183C)|FD-Score:3.1|P-value:9.64E-4||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:GCG1(YER163C_p)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle Gene:HSE1(YHL002W)|FD-Score:-3.36|P-value:3.94E-4||SGD DESC:Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes Gene:LOC1(YFR001W)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:MAP1(YLR244C)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p Gene:MMS22(YLR320W)|FD-Score:4.71|P-value:1.24E-6||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation Gene:NSR1(YGR159C)|FD-Score:-3.99|P-value:3.35E-5||SGD DESC:Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis Gene:PAC2(YER007W)|FD-Score:4.08|P-value:2.25E-5||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PPT2(YPL148C)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:RSA1(YPL193W)|FD-Score:-3.71|P-value:1.06E-4||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:SEC66(YBR171W)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SNF7(YLR025W)|FD-Score:-3.98|P-value:3.45E-5||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SOL2(YCR073W-A)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:TLG2(YOL018C)|FD-Score:-3.15|P-value:8.06E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TRP4(YDR354W)|FD-Score:5.88|P-value:2.04E-9||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:VPS69(YPR087W_d)|FD-Score:-3.1|P-value:9.62E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect Gene:YAL066W(YAL066W_d)|FD-Score:-3.38|P-value:3.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YAP1801(YHR161C)|FD-Score:3.75|P-value:8.85E-5||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication Gene:YBR241C(YBR241C_p)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:Putative transporter, member of the sugar porter family; green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane; YBR241C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:4.28|P-value:9.53E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR056C(YDR056C_p)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein Gene:YEL028W(YEL028W_d)|FD-Score:4.96|P-value:3.56E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YET1(YKL065C)|FD-Score:3.97|P-value:3.57E-5||SGD DESC:Endoplasmic reticulum transmembrane protein; may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein Gene:YHR112C(YHR112C)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Protein of unknown function; localizes to the cytoplasm and nucleus; overexpression affects protein trafficking through the endocytic pathway Gene:YLR407W(YLR407W_p)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Putative protein of unknown function; null mutant displays elongated buds and a large fraction of budded cells have only one nucleus Gene:YLR455W(YLR455W_p)|FD-Score:4.68|P-value:1.44E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress Gene:CBF1(YJR060W)|FD-Score:-3.12|P-value:9.18E-4||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:CIN1(YOR349W)|FD-Score:4.65|P-value:1.63E-6||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:CMR1(YDL156W)|FD-Score:3.14|P-value:8.50E-4||SGD DESC:DNA-binding protein with preference for UV-damaged DNA; protein sequence contains three WD domains (WD-40 repeat); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; potential regulatory target of Mbp1p, which binds to the promoter region; co-localizes with Hos2p in nuclear foci in response to DNA damage by MMS Gene:CPA2(YJR109C)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Large subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor Gene:CST26(YBR042C)|FD-Score:6.92|P-value:2.20E-12||SGD DESC:Putative transferase involved in phospholipid biosynthesis; required for incorporation of stearic acid into phosphatidylinositol; affects chromosome stability when overexpressed; CST26 has a paralog, YDR018C, that arose from the whole genome duplication Gene:ERD1(YDR414C)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) Gene:FAU1(YER183C)|FD-Score:3.1|P-value:9.64E-4||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:GCG1(YER163C_p)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle Gene:HSE1(YHL002W)|FD-Score:-3.36|P-value:3.94E-4||SGD DESC:Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes Gene:LOC1(YFR001W)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:MAP1(YLR244C)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p Gene:MMS22(YLR320W)|FD-Score:4.71|P-value:1.24E-6||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation Gene:NSR1(YGR159C)|FD-Score:-3.99|P-value:3.35E-5||SGD DESC:Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis Gene:PAC2(YER007W)|FD-Score:4.08|P-value:2.25E-5||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PPT2(YPL148C)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:RSA1(YPL193W)|FD-Score:-3.71|P-value:1.06E-4||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:SEC66(YBR171W)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SNF7(YLR025W)|FD-Score:-3.98|P-value:3.45E-5||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SOL2(YCR073W-A)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:TLG2(YOL018C)|FD-Score:-3.15|P-value:8.06E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TRP4(YDR354W)|FD-Score:5.88|P-value:2.04E-9||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:VPS69(YPR087W_d)|FD-Score:-3.1|P-value:9.62E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect Gene:YAL066W(YAL066W_d)|FD-Score:-3.38|P-value:3.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YAP1801(YHR161C)|FD-Score:3.75|P-value:8.85E-5||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication Gene:YBR241C(YBR241C_p)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:Putative transporter, member of the sugar porter family; green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane; YBR241C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:4.28|P-value:9.53E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR056C(YDR056C_p)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein Gene:YEL028W(YEL028W_d)|FD-Score:4.96|P-value:3.56E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YET1(YKL065C)|FD-Score:3.97|P-value:3.57E-5||SGD DESC:Endoplasmic reticulum transmembrane protein; may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein Gene:YHR112C(YHR112C)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Protein of unknown function; localizes to the cytoplasm and nucleus; overexpression affects protein trafficking through the endocytic pathway Gene:YLR407W(YLR407W_p)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Putative protein of unknown function; null mutant displays elongated buds and a large fraction of budded cells have only one nucleus Gene:YLR455W(YLR455W_p)|FD-Score:4.68|P-value:1.44E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOL139C4.741.04E-60.56CDC33mRNA cap binding protein and translation initiation factor eIF4E; the eIF4E-cap complex is responsible for mediating cap-dependent mRNA translation via interactions with translation initiation factor eIF4G (Tif4631p or Tif4632p); protein abundance increases in response to DNA replication stress; mutants are defective for adhesion and pseudohyphal growth
YHR042W4.503.44E-60.56NCP1NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p
YHR074W4.042.68E-50.56QNS1Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide
YBR136W3.934.33E-50.56MEC1Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres
YER112W3.749.23E-50.56LSM4Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress
YGL169W3.681.16E-40.56SUA5Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family
YPL117C3.542.01E-40.56IDI1Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability
YGR116W2.980.001440.07SPT6Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region
YLR259C2.910.001820.02HSP60Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; phosphorylated
YPR180W2.890.001920.05AOS1Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
YDL060W2.850.002210.07TSR1Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress
YLR010C2.780.002710.07TEN1Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p
YLR127C2.720.003310.06APC2Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p
YOR250C2.650.004010.14CLP1Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation
YOR057W2.510.006050.04SGT1Cochaperone protein; regulates activity of adenylyl cyclase Cyr1p; involved in kinetochore complex assembly; associates with the SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase complex; acts as a linker between Skp1p and HSP90 complexes; protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR042C6.922.20E-12CST26Putative transferase involved in phospholipid biosynthesis; required for incorporation of stearic acid into phosphatidylinositol; affects chromosome stability when overexpressed; CST26 has a paralog, YDR018C, that arose from the whole genome duplication
YDR354W5.882.04E-9TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YEL028W_d4.963.56E-7YEL028W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YLR320W4.711.24E-6MMS22Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation
YLR455W_p4.681.44E-6YLR455W_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress
YOR349W4.651.63E-6CIN1Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl
YDR008C_d4.289.53E-6YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YER007W4.082.25E-5PAC2Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl
YKL065C3.973.57E-5YET1Endoplasmic reticulum transmembrane protein; may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein
YHR161C3.758.85E-5YAP1801Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication
YHR112C3.711.04E-4YHR112CProtein of unknown function; localizes to the cytoplasm and nucleus; overexpression affects protein trafficking through the endocytic pathway
YLR407W_p3.571.77E-4YLR407W_pPutative protein of unknown function; null mutant displays elongated buds and a large fraction of budded cells have only one nucleus
YJR109C3.561.86E-4CPA2Large subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor
YCR073W-A3.462.75E-4SOL2Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication
YBR171W3.423.09E-4SEC66Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER

GO enrichment analysis for SGTC_3024
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1575.52E-34SGTC_229benomyl 22.9 μMMiscellaneous287800.102564tubulin folding & SWR complex
0.1411.09E-27SGTC_2741miltefosine 3.0 μMMiscellaneous35990.0294118
0.1374.23E-26SGTC_511013-0126 23.4 μMChemDiv (Drug-like library)16278500.0921053
0.1331.07E-24SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0555556
0.1301.20E-23SGTC_305-(2-thienylidene)-Rhodanine 28.7 μMChemDiv (Drug-like library)12411320.0882353
0.1291.74E-23SGTC_2733amorolfine 100.0 μMMiscellaneous542600.109589plasma membrane duress
0.1293.53E-23SGTC_1778st046819 58.9 μMTimTec (Natural product derivative library)6717150.0843373
0.1241.20E-21SGTC_21755807670 165.3 μMChembridge (Fragment library)2411860.0735294
0.1217.19E-21SGTC_2618avocadyne 43.4 μMMicrosource (Natural product library)3015189NA
0.1202.43E-20SGTC_13521496-0991 99.0 μMChemDiv (Drug-like library)30000390.107692tubulin folding & SWR complex
0.1193.86E-20SGTC_24865283202 22.4 μMMiscellaneous13777510.0746269
0.1195.27E-20SGTC_2669eugenol 100.0 μMMicrosource (Natural product library)33140.0606061
0.1181.09E-19SGTC_2524avocatin b 10.6 μMMicrosource (Natural product library)6710748NA
0.1172.70E-19SGTC_24885268135 45.7 μMMiscellaneous12703560.0724638
0.1156.88E-19SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.0958904

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3057909594649.47 μM0.61538524540043Chembridge (Drug-like library)311.8021034.43603Golgi
SGTC_3037909102649.47 μM0.4262325236398Chembridge (Drug-like library)387.722343.71413NEO1-PIK1
SGTC_3147909702749.47 μM0.39655225236841Chembridge (Drug-like library)299.430444.66202
SGTC_3021908765849.47 μM0.33898325237042Chembridge (Drug-like library)275.38593.58802
SGTC_2985902237571.43 μM0.3281254939335Chembridge (Drug-like library)350.454043.68812RPP1 & pyrimidine depletion
SGTC_297190921869.93 μM0.30769225236400Chembridge (Drug-like library)343.271343.6313ergosterol biosynthesis
SGTC_3161910038849.47 μM0.30769225236368Chembridge (Drug-like library)381.67474.03713iron homeostasis
SGTC_3124092-06536.84 μM0.3064521148278ChemDiv (Drug-like library)366.4716834.54903ergosterol depletion effects on membrane
SGTC_3152909925349.47 μM0.3030325236131Chembridge (Drug-like library)322.852863.45213NEO1-PIK1
SGTC_2795778001054.55 μM0.301587953673Chembridge (Drug-like library)312.3812434.15113