9088683

1-[4-(2-methylphenyl)piperazin-1-yl]-2-thiophen-2-ylethanone

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3029
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 25236615
SMILES CC1=CC=CC=C1N2CCN(CC2)C(=O)CC3=CC=CS3
Standardized SMILES Cc1ccccc1N2CCN(CC2)C(=O)Cc3cccs3
Molecular weight 300.4185
ALogP 3.35
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.8
% growth inhibition (Hom. pool) 8.86


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 25236615
Download HIP data (tab-delimited text)  (excel)
Gene:BET4(YJL031C)|FD-Score:3.45|P-value:2.80E-4|Clearance:0.01||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CDC5(YMR001C)|FD-Score:3.68|P-value:1.16E-4|Clearance:0.12||SGD DESC:Polo-like kinase with multiple functions in mitosis and cytokinesis through substrate phosphorylation, also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate Gene:DBP2(YNL112W)|FD-Score:4.12|P-value:1.87E-5|Clearance:0.44||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:DSL1(YNL258C)|FD-Score:3.44|P-value:2.90E-4|Clearance:0.21||SGD DESC:Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p and Tip20p that interacts with ER SNAREs Sec20p and Use1p; component of the ER target site that interacts with coatomer; interacts with Cin5p; similar to the fly and human ZW10 gene Gene:GNA1(YFL017C)|FD-Score:4.81|P-value:7.60E-7|Clearance:0.52||SGD DESC:Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA Gene:GUK1(YDR454C)|FD-Score:-3.26|P-value:5.64E-4|Clearance:0||SGD DESC:Guanylate kinase, converts GMP to GDP; required for growth and mannose outer chain elongation of cell wall N-linked glycoproteins Gene:HEM4(YOR278W)|FD-Score:-3.22|P-value:6.48E-4|Clearance:0||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:HRR25(YPL204W)|FD-Score:4.64|P-value:1.75E-6|Clearance:0.52||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:MAS1(YLR163C)|FD-Score:3.11|P-value:9.25E-4|Clearance:0.04||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:NAR1(YNL240C)|FD-Score:3.13|P-value:8.62E-4|Clearance:0.02||SGD DESC:Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf Gene:PDS5(YMR076C)|FD-Score:-5.36|P-value:4.12E-8|Clearance:0||SGD DESC:Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis Gene:PRP5(YBR237W)|FD-Score:3.57|P-value:1.81E-4|Clearance:0||SGD DESC:RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA Gene:RLP24(YLR009W)|FD-Score:3.19|P-value:7.06E-4|Clearance:0.06||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPP1(YHR062C)|FD-Score:-3.61|P-value:1.54E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SNU71(YGR013W)|FD-Score:-3.31|P-value:4.60E-4|Clearance:0||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart Gene:SPB1(YCL054W)|FD-Score:-3.58|P-value:1.70E-4|Clearance:0||SGD DESC:AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants Gene:SPC98(YNL126W)|FD-Score:3.23|P-value:6.10E-4|Clearance:0.04||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:TIM22(YDL217C)|FD-Score:-3.31|P-value:4.68E-4|Clearance:0||SGD DESC:Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported Gene:YGR114C(YGR114C_d)|FD-Score:3.56|P-value:1.84E-4|Clearance:0.11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YLL037W(YLL037W_d)|FD-Score:-3.27|P-value:5.33E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential PRP19 gene encoding an RNA splicing factor Gene:YPL238C(YPL238C_d)|FD-Score:-4.27|P-value:9.71E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:BET4(YJL031C)|FD-Score:3.45|P-value:2.80E-4|Clearance:0.01||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CDC5(YMR001C)|FD-Score:3.68|P-value:1.16E-4|Clearance:0.12||SGD DESC:Polo-like kinase with multiple functions in mitosis and cytokinesis through substrate phosphorylation, also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate Gene:DBP2(YNL112W)|FD-Score:4.12|P-value:1.87E-5|Clearance:0.44||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:DSL1(YNL258C)|FD-Score:3.44|P-value:2.90E-4|Clearance:0.21||SGD DESC:Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p and Tip20p that interacts with ER SNAREs Sec20p and Use1p; component of the ER target site that interacts with coatomer; interacts with Cin5p; similar to the fly and human ZW10 gene Gene:GNA1(YFL017C)|FD-Score:4.81|P-value:7.60E-7|Clearance:0.52||SGD DESC:Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA Gene:GUK1(YDR454C)|FD-Score:-3.26|P-value:5.64E-4|Clearance:0||SGD DESC:Guanylate kinase, converts GMP to GDP; required for growth and mannose outer chain elongation of cell wall N-linked glycoproteins Gene:HEM4(YOR278W)|FD-Score:-3.22|P-value:6.48E-4|Clearance:0||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:HRR25(YPL204W)|FD-Score:4.64|P-value:1.75E-6|Clearance:0.52||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:MAS1(YLR163C)|FD-Score:3.11|P-value:9.25E-4|Clearance:0.04||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:NAR1(YNL240C)|FD-Score:3.13|P-value:8.62E-4|Clearance:0.02||SGD DESC:Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf Gene:PDS5(YMR076C)|FD-Score:-5.36|P-value:4.12E-8|Clearance:0||SGD DESC:Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis Gene:PRP5(YBR237W)|FD-Score:3.57|P-value:1.81E-4|Clearance:0||SGD DESC:RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA Gene:RLP24(YLR009W)|FD-Score:3.19|P-value:7.06E-4|Clearance:0.06||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPP1(YHR062C)|FD-Score:-3.61|P-value:1.54E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SNU71(YGR013W)|FD-Score:-3.31|P-value:4.60E-4|Clearance:0||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart Gene:SPB1(YCL054W)|FD-Score:-3.58|P-value:1.70E-4|Clearance:0||SGD DESC:AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants Gene:SPC98(YNL126W)|FD-Score:3.23|P-value:6.10E-4|Clearance:0.04||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:TIM22(YDL217C)|FD-Score:-3.31|P-value:4.68E-4|Clearance:0||SGD DESC:Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported Gene:YGR114C(YGR114C_d)|FD-Score:3.56|P-value:1.84E-4|Clearance:0.11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YLL037W(YLL037W_d)|FD-Score:-3.27|P-value:5.33E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential PRP19 gene encoding an RNA splicing factor Gene:YPL238C(YPL238C_d)|FD-Score:-4.27|P-value:9.71E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 25236615
Download HOP data (tab-delimited text)  (excel)
Gene:ADE17(YMR120C)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE17 has a paralog, ADE16, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine Gene:APM3(YBR288C)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway Gene:ASM4(YDL088C)|FD-Score:3.83|P-value:6.35E-5||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication Gene:ATP11(YNL315C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Molecular chaperone, required for the assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase Gene:BNA1(YJR025C)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:3-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:BTS1(YPL069C)|FD-Score:-4.02|P-value:2.86E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CHA1(YCL064C)|FD-Score:4.56|P-value:2.52E-6||SGD DESC:Catabolic L-serine (L-threonine) deaminase, catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptionally induced by serine and threonine Gene:CHS7(YHR142W)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:CLN3(YAL040C)|FD-Score:3.12|P-value:9.01E-4||SGD DESC:G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis Gene:CRP1(YHR146W)|FD-Score:4.98|P-value:3.13E-7||SGD DESC:Protein that binds to cruciform DNA structures Gene:CYB5(YNL111C)|FD-Score:-3.11|P-value:9.43E-4||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:DIC1(YLR348C)|FD-Score:4.86|P-value:5.94E-7||SGD DESC:Mitochondrial dicarboxylate carrier, integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix Gene:DYN2(YDR424C)|FD-Score:3.1|P-value:9.65E-4||SGD DESC:Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex Gene:EDS1(YBR033W_p)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication Gene:EMI2(YDR516C)|FD-Score:3.15|P-value:8.09E-4||SGD DESC:Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:GTR1(YML121W)|FD-Score:-3.18|P-value:7.44E-4||SGD DESC:Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB Gene:HAL1(YPR005C)|FD-Score:3.11|P-value:9.35E-4||SGD DESC:Cytoplasmic protein involved in halotolerance; decreases intracellular Na+ (via Ena1p) and increases intracellular K+ by decreasing efflux; expression repressed by Ssn6p-Tup1p and Sko1p and induced by NaCl, KCl, and sorbitol through Gcn4p Gene:ICE2(YIL090W)|FD-Score:3.9|P-value:4.77E-5||SGD DESC:Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells Gene:IES2(YNL215W)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress Gene:IES5(YER092W)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions Gene:INM1(YHR046C)|FD-Score:5.68|P-value:6.85E-9||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate Gene:LCL1(YPL056C_p)|FD-Score:4.69|P-value:1.36E-6||SGD DESC:Putative protein of unknown function; deletion mutant is fluconazole resistant and has long chronological lifespan Gene:MFB1(YDR219C)|FD-Score:4.8|P-value:8.07E-7||SGD DESC:Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding Gene:MFM1(YPL060W)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p Gene:MGA2(YIR033W)|FD-Score:-6.1|P-value:5.37E-10||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:MMS22(YLR320W)|FD-Score:-3.18|P-value:7.48E-4||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation Gene:MNN10(YDR245W)|FD-Score:-4.81|P-value:7.48E-7||SGD DESC:Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family Gene:MPP6(YNR024W)|FD-Score:3.1|P-value:9.58E-4||SGD DESC:Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes Gene:MRI1(YPR118W)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway Gene:NDE2(YDL085W)|FD-Score:4.51|P-value:3.18E-6||SGD DESC:Mitochondrial external NADH dehydrogenase, catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p are involved in providing the cytosolic NADH to the mitochondrial respiratory chain Gene:OPY2(YPR075C)|FD-Score:3.09|P-value:9.86E-4||SGD DESC:Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress Gene:PET123(YOR158W)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator Gene:PEX32(YBR168W)|FD-Score:3.89|P-value:5.11E-5||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PTC1(YDL006W)|FD-Score:6.12|P-value:4.72E-10||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:PTH1(YHR189W)|FD-Score:5.46|P-value:2.42E-8||SGD DESC:One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium Gene:PXL1(YKR090W)|FD-Score:3.15|P-value:8.04E-4||SGD DESC:Protein that localizes to sites of polarized growth; required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; contains LIM domains and has similarity to metazoan paxillin; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:QRI7(YDL104C)|FD-Score:5.23|P-value:8.48E-8||SGD DESC:Highly conserved mitochondrial protein, essential for t6A modification of mitochondrial tRNAs that decode ANN codons; similar to Kae1p and E. coli YgjD, both of which are also required for tRNA t6A modification Gene:RGT1(YKL038W)|FD-Score:-3.38|P-value:3.68E-4||SGD DESC:Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; RGT1 has a paralog, EDS1, that arose from the whole genome duplication Gene:RHO4(YKR055W)|FD-Score:4.4|P-value:5.30E-6||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely to be involved in the establishment of cell polarity Gene:RNH201(YNL072W)|FD-Score:3.19|P-value:7.22E-4||SGD DESC:Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome Gene:RPS30A(YLR287C-A)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication Gene:RSA1(YPL193W)|FD-Score:4.38|P-value:5.92E-6||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:SAK1(YER129W)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome Gene:SGO1(YOR073W)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability Gene:SHB17(YKR043C)|FD-Score:4.32|P-value:7.78E-6||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SSA3(YBL075C)|FD-Score:5.86|P-value:2.30E-9||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:TDA6(YPR157W_p)|FD-Score:-3.11|P-value:9.33E-4||SGD DESC:Putative protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; null mutant is sensitive to expression of the top1-T722A allele Gene:THI6(YPL214C)|FD-Score:-3.15|P-value:8.30E-4||SGD DESC:Bifunctional enzyme with thiamine-phosphate pyrophosphorylase and 4-methyl-5-beta-hydroxyethylthiazole kinase activities, required for thiamine biosynthesis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:TMA19(YKL056C)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress Gene:UFO1(YML088W)|FD-Score:6.43|P-value:6.22E-11||SGD DESC:F-box receptor protein, subunit of the Skp1-Cdc53-F-box receptor (SCF) E3 ubiquitin ligase complex; binds to phosphorylated Ho endonuclease, allowing its ubiquitylation by SCF and subsequent degradation Gene:VMS1(YDR049W)|FD-Score:-3.33|P-value:4.38E-4||SGD DESC:Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans Gene:VPS1(YKR001C)|FD-Score:-7.67|P-value:8.76E-15||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:YBL039W-B(YBL039W-B_p)|FD-Score:-3.86|P-value:5.56E-5||SGD DESC:Putative protein of unknown function Gene:YBL059W(YBL059W_p)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication Gene:YCR085W(YCR085W_d)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER158C(YER158C_p)|FD-Score:4.78|P-value:8.80E-7||SGD DESC:Protein of unknown function; potentially phosphorylated by Cdc28p; YER158C has a paralog, AFR1, that arose from the whole genome duplication Gene:YFR012W-A(YFR012W-A_p)|FD-Score:3.17|P-value:7.69E-4||SGD DESC:Putative protein of unknown function; identified by homology Gene:YGL042C(YGL042C_d)|FD-Score:3.14|P-value:8.34E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene DST1 Gene:YIR018C-A(YIR018C-A_p)|FD-Score:4.12|P-value:1.85E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YLR001C(YLR001C_p)|FD-Score:4.38|P-value:6.00E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated Gene:YLR271W(YLR271W_p)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YLR455W(YLR455W_p)|FD-Score:6.24|P-value:2.17E-10||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress Gene:YMR084W(YMR084W_p)|FD-Score:6.88|P-value:3.09E-12||SGD DESC:Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YPR196W(YPR196W_p)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Putative maltose-responsive transcription factor Gene:YSC83(YHR017W)|FD-Score:6.78|P-value:5.83E-12||SGD DESC:Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83 Gene:YVH1(YIR026C)|FD-Score:3.79|P-value:7.43E-5||SGD DESC:Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases Gene:ADE17(YMR120C)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE17 has a paralog, ADE16, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine Gene:APM3(YBR288C)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway Gene:ASM4(YDL088C)|FD-Score:3.83|P-value:6.35E-5||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication Gene:ATP11(YNL315C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Molecular chaperone, required for the assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase Gene:BNA1(YJR025C)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:3-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:BTS1(YPL069C)|FD-Score:-4.02|P-value:2.86E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CHA1(YCL064C)|FD-Score:4.56|P-value:2.52E-6||SGD DESC:Catabolic L-serine (L-threonine) deaminase, catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptionally induced by serine and threonine Gene:CHS7(YHR142W)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:CLN3(YAL040C)|FD-Score:3.12|P-value:9.01E-4||SGD DESC:G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis Gene:CRP1(YHR146W)|FD-Score:4.98|P-value:3.13E-7||SGD DESC:Protein that binds to cruciform DNA structures Gene:CYB5(YNL111C)|FD-Score:-3.11|P-value:9.43E-4||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:DIC1(YLR348C)|FD-Score:4.86|P-value:5.94E-7||SGD DESC:Mitochondrial dicarboxylate carrier, integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix Gene:DYN2(YDR424C)|FD-Score:3.1|P-value:9.65E-4||SGD DESC:Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex Gene:EDS1(YBR033W_p)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication Gene:EMI2(YDR516C)|FD-Score:3.15|P-value:8.09E-4||SGD DESC:Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:GTR1(YML121W)|FD-Score:-3.18|P-value:7.44E-4||SGD DESC:Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB Gene:HAL1(YPR005C)|FD-Score:3.11|P-value:9.35E-4||SGD DESC:Cytoplasmic protein involved in halotolerance; decreases intracellular Na+ (via Ena1p) and increases intracellular K+ by decreasing efflux; expression repressed by Ssn6p-Tup1p and Sko1p and induced by NaCl, KCl, and sorbitol through Gcn4p Gene:ICE2(YIL090W)|FD-Score:3.9|P-value:4.77E-5||SGD DESC:Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells Gene:IES2(YNL215W)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress Gene:IES5(YER092W)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions Gene:INM1(YHR046C)|FD-Score:5.68|P-value:6.85E-9||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate Gene:LCL1(YPL056C_p)|FD-Score:4.69|P-value:1.36E-6||SGD DESC:Putative protein of unknown function; deletion mutant is fluconazole resistant and has long chronological lifespan Gene:MFB1(YDR219C)|FD-Score:4.8|P-value:8.07E-7||SGD DESC:Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding Gene:MFM1(YPL060W)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p Gene:MGA2(YIR033W)|FD-Score:-6.1|P-value:5.37E-10||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:MMS22(YLR320W)|FD-Score:-3.18|P-value:7.48E-4||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation Gene:MNN10(YDR245W)|FD-Score:-4.81|P-value:7.48E-7||SGD DESC:Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family Gene:MPP6(YNR024W)|FD-Score:3.1|P-value:9.58E-4||SGD DESC:Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes Gene:MRI1(YPR118W)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway Gene:NDE2(YDL085W)|FD-Score:4.51|P-value:3.18E-6||SGD DESC:Mitochondrial external NADH dehydrogenase, catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p are involved in providing the cytosolic NADH to the mitochondrial respiratory chain Gene:OPY2(YPR075C)|FD-Score:3.09|P-value:9.86E-4||SGD DESC:Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress Gene:PET123(YOR158W)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator Gene:PEX32(YBR168W)|FD-Score:3.89|P-value:5.11E-5||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PTC1(YDL006W)|FD-Score:6.12|P-value:4.72E-10||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:PTH1(YHR189W)|FD-Score:5.46|P-value:2.42E-8||SGD DESC:One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium Gene:PXL1(YKR090W)|FD-Score:3.15|P-value:8.04E-4||SGD DESC:Protein that localizes to sites of polarized growth; required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; contains LIM domains and has similarity to metazoan paxillin; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:QRI7(YDL104C)|FD-Score:5.23|P-value:8.48E-8||SGD DESC:Highly conserved mitochondrial protein, essential for t6A modification of mitochondrial tRNAs that decode ANN codons; similar to Kae1p and E. coli YgjD, both of which are also required for tRNA t6A modification Gene:RGT1(YKL038W)|FD-Score:-3.38|P-value:3.68E-4||SGD DESC:Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; RGT1 has a paralog, EDS1, that arose from the whole genome duplication Gene:RHO4(YKR055W)|FD-Score:4.4|P-value:5.30E-6||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely to be involved in the establishment of cell polarity Gene:RNH201(YNL072W)|FD-Score:3.19|P-value:7.22E-4||SGD DESC:Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome Gene:RPS30A(YLR287C-A)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication Gene:RSA1(YPL193W)|FD-Score:4.38|P-value:5.92E-6||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:SAK1(YER129W)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome Gene:SGO1(YOR073W)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability Gene:SHB17(YKR043C)|FD-Score:4.32|P-value:7.78E-6||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SSA3(YBL075C)|FD-Score:5.86|P-value:2.30E-9||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:TDA6(YPR157W_p)|FD-Score:-3.11|P-value:9.33E-4||SGD DESC:Putative protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; null mutant is sensitive to expression of the top1-T722A allele Gene:THI6(YPL214C)|FD-Score:-3.15|P-value:8.30E-4||SGD DESC:Bifunctional enzyme with thiamine-phosphate pyrophosphorylase and 4-methyl-5-beta-hydroxyethylthiazole kinase activities, required for thiamine biosynthesis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:TMA19(YKL056C)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress Gene:UFO1(YML088W)|FD-Score:6.43|P-value:6.22E-11||SGD DESC:F-box receptor protein, subunit of the Skp1-Cdc53-F-box receptor (SCF) E3 ubiquitin ligase complex; binds to phosphorylated Ho endonuclease, allowing its ubiquitylation by SCF and subsequent degradation Gene:VMS1(YDR049W)|FD-Score:-3.33|P-value:4.38E-4||SGD DESC:Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans Gene:VPS1(YKR001C)|FD-Score:-7.67|P-value:8.76E-15||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:YBL039W-B(YBL039W-B_p)|FD-Score:-3.86|P-value:5.56E-5||SGD DESC:Putative protein of unknown function Gene:YBL059W(YBL059W_p)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication Gene:YCR085W(YCR085W_d)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER158C(YER158C_p)|FD-Score:4.78|P-value:8.80E-7||SGD DESC:Protein of unknown function; potentially phosphorylated by Cdc28p; YER158C has a paralog, AFR1, that arose from the whole genome duplication Gene:YFR012W-A(YFR012W-A_p)|FD-Score:3.17|P-value:7.69E-4||SGD DESC:Putative protein of unknown function; identified by homology Gene:YGL042C(YGL042C_d)|FD-Score:3.14|P-value:8.34E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene DST1 Gene:YIR018C-A(YIR018C-A_p)|FD-Score:4.12|P-value:1.85E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YLR001C(YLR001C_p)|FD-Score:4.38|P-value:6.00E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated Gene:YLR271W(YLR271W_p)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YLR455W(YLR455W_p)|FD-Score:6.24|P-value:2.17E-10||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress Gene:YMR084W(YMR084W_p)|FD-Score:6.88|P-value:3.09E-12||SGD DESC:Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YPR196W(YPR196W_p)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Putative maltose-responsive transcription factor Gene:YSC83(YHR017W)|FD-Score:6.78|P-value:5.83E-12||SGD DESC:Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83 Gene:YVH1(YIR026C)|FD-Score:3.79|P-value:7.43E-5||SGD DESC:Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YFL017C4.817.60E-70.52GNA1Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA
YPL204W4.641.75E-60.52HRR25Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta)
YNL112W4.121.87E-50.44DBP2ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites
YMR001C3.681.16E-40.12CDC5Polo-like kinase with multiple functions in mitosis and cytokinesis through substrate phosphorylation, also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate
YBR237W3.571.81E-40.00PRP5RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA
YGR114C_d3.561.84E-40.11YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YJL031C3.452.80E-40.01BET4Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p
YNL258C3.442.90E-40.21DSL1Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p and Tip20p that interacts with ER SNAREs Sec20p and Use1p; component of the ER target site that interacts with coatomer; interacts with Cin5p; similar to the fly and human ZW10 gene
YNL126W3.236.10E-40.04SPC98Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
YLR009W3.197.06E-40.06RLP24Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
YNL240C3.138.62E-40.02NAR1Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf
YLR163C3.119.25E-40.04MAS1Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
YOR244W3.080.001050.01ESA1Catalytic subunit of the histone acetyltransferase complex (NuA4); acetylates four conserved internal lysines of histone H4 N-terminal tail and can acetylate histone H2A; required for cell cycle progression and transcriptional silencing at the rDNA locus and regulation of autophagy
YNR053C3.070.001080.13NOG2Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation
YDL150W2.940.001640.04RPC53RNA polymerase III subunit C53

Top 15 HOM fitness defect scores (HOP)

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Deletion Strain FD score P-value Gene Gene Description
YMR084W_p6.883.09E-12YMR084W_pPutative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1
YHR017W6.785.83E-12YSC83Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83
YML088W6.436.22E-11UFO1F-box receptor protein, subunit of the Skp1-Cdc53-F-box receptor (SCF) E3 ubiquitin ligase complex; binds to phosphorylated Ho endonuclease, allowing its ubiquitylation by SCF and subsequent degradation
YLR455W_p6.242.17E-10YLR455W_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress
YDL006W6.124.72E-10PTC1Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation
YBL075C5.862.30E-9SSA3ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication
YHR046C5.686.85E-9INM1Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate
YHR189W5.462.42E-8PTH1One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium
YDL104C5.238.48E-8QRI7Highly conserved mitochondrial protein, essential for t6A modification of mitochondrial tRNAs that decode ANN codons; similar to Kae1p and E. coli YgjD, both of which are also required for tRNA t6A modification
YHR146W4.983.13E-7CRP1Protein that binds to cruciform DNA structures
YLR348C4.865.94E-7DIC1Mitochondrial dicarboxylate carrier, integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix
YDR219C4.808.07E-7MFB1Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding
YER158C_p4.788.80E-7YER158C_pProtein of unknown function; potentially phosphorylated by Cdc28p; YER158C has a paralog, AFR1, that arose from the whole genome duplication
YPL056C_p4.691.36E-6LCL1_pPutative protein of unknown function; deletion mutant is fluconazole resistant and has long chronological lifespan
YCL064C4.562.52E-6CHA1Catabolic L-serine (L-threonine) deaminase, catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptionally induced by serine and threonine

GO enrichment analysis for SGTC_3029
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0723.57E-8SGTC_2170morpholine 200.0 μMChembridge (Fragment library)1211710.196721
0.0715.15E-8SGTC_1073triclabendazole 3.1 μMNIH Clinical Collection502480.0675676
0.0663.73E-7SGTC_30349090421 49.5 μMChembridge (Drug-like library)171737610.1260S ribosome export
0.0648.73E-7SGTC_9891501-0895 10.3 μMChemDiv (Drug-like library)28301120.0810811
0.0621.87E-6SGTC_29309013230 14.9 μMChembridge (Drug-like library)29958570.1heme biosynthesis & mitochondrial translocase
0.0604.28E-6SGTC_950471-0001 561.1 μMChemDiv (Drug-like library)40154050.121212
0.0594.79E-6SGTC_33339141820 12.1 μMChembridge (Drug-like library)19394330.166667
0.0595.42E-6SGTC_30309087619 49.5 μMChembridge (Drug-like library)252369730.177419
0.0587.34E-6SGTC_2551apiole 73.9 μMMicrosource (Natural product library)106590.0746269
0.0571.33E-5SGTC_30399090270 49.5 μMChembridge (Drug-like library)171737500.117647RPP1 & pyrimidine depletion
0.0561.43E-5SGTC_1606st000094 49.2 μMTimTec (Natural product derivative library)12422100.18918960S ribosome export
0.0561.57E-5SGTC_14384092-1045 4.0 μMChemDiv (Drug-like library)13513420.0655738
0.0552.49E-5SGTC_20965468907 200.0 μMChembridge (Fragment library)7597850.121212
0.0525.61E-5SGTC_11780988-0033 191.0 μMChemDiv (Drug-like library)53922070.0945946
0.0525.89E-5SGTC_1749st037932 54.2 μMTimTec (Natural product derivative library)5944970.0897436

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3036909018149.47 μM0.50980425236586Chembridge (Drug-like library)314.445083.65303
SGTC_3046909221249.47 μM0.50943425236637Chembridge (Drug-like library)328.3806433.404
SGTC_3156909993149.47 μM0.50943418111053Chembridge (Drug-like library)344.835243.85903
SGTC_3143908665849.47 μM0.525236626Chembridge (Drug-like library)300.41853.45803
SGTC_3149909785549.47 μM0.48076925236625Chembridge (Drug-like library)365.287983.88103Golgi
SGTC_22016605497169.86 μM0.4375670741Chembridge (Fragment library)232.32142.18102ergosterol biosynthesis
SGTC_3056909490049.47 μM0.42105325236784Chembridge (Drug-like library)342.4072233.88404
SGTC_22267298446200 μM0.3620693283010Chembridge (Fragment library)287.379982.70104
SGTC_6164092-0450108 μM0.345455787165ChemDiv (Drug-like library)322.443943.76202
SGTC_22677931562200 μM0.339286934880Chembridge (Fragment library)267.754521.79313