1348-1668

4-bromo-6-[(2-pyridin-2-ylhydrazinyl)methylidene]cyclohexa-2,4-dien-1-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_303
Screen concentration 34.2 μM
Source ChemDiv (Drug-like library)
PubChem CID 5380509
SMILES C1=CC=NC(=C1)NNC=C2C=C(C=CC2=O)Br
Standardized SMILES Oc1ccc(Br)cc1C=NNc2ccccn2
Molecular weight 292.1313
ALogP 1.61
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.76
% growth inhibition (Hom. pool) -1.21


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5380509
Download HIP data (tab-delimited text)  (excel)
Gene:ALA1(YOR335C)|FD-Score:3.27|P-value:5.33E-4|Clearance:0.05||SGD DESC:Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog Gene:FHL1(YPR104C)|FD-Score:3.58|P-value:1.72E-4|Clearance:0.31||SGD DESC:Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants Gene:NIC96(YFR002W)|FD-Score:3.18|P-value:7.47E-4|Clearance:0.04||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:POP4(YBR257W)|FD-Score:4.1|P-value:2.02E-5|Clearance:0.32||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:PRP40(YKL012W)|FD-Score:3.78|P-value:7.73E-5|Clearance:0.14||SGD DESC:U1 snRNP protein involved in splicing, interacts with the branchpoint-binding protein during the formation of the second commitment complex Gene:RNR2(YJL026W)|FD-Score:5.4|P-value:3.24E-8|Clearance:0.96||SGD DESC:Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits Gene:RPB2(YOR151C)|FD-Score:3.12|P-value:9.00E-4|Clearance:0.05||SGD DESC:RNA polymerase II second largest subunit B150, part of central core; similar to bacterial beta subunit Gene:SEC39(YLR440C)|FD-Score:3.64|P-value:1.37E-4|Clearance:0.01||SGD DESC:Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope Gene:SNM1(YDR478W)|FD-Score:3.14|P-value:8.55E-4|Clearance:0.01||SGD DESC:Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP Gene:SPB1(YCL054W)|FD-Score:3.13|P-value:8.86E-4|Clearance:0||SGD DESC:AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants Gene:STU1(YBL034C)|FD-Score:-3.12|P-value:9.00E-4|Clearance:0||SGD DESC:Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles Gene:TAF9(YMR236W)|FD-Score:3.22|P-value:6.31E-4|Clearance:0.05||SGD DESC:Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3 Gene:YGL074C(YGL074C_d)|FD-Score:3.63|P-value:1.40E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor Gene:YPT1(YFL038C)|FD-Score:4.44|P-value:4.44E-6|Clearance:0.34||SGD DESC:Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p) Gene:ALA1(YOR335C)|FD-Score:3.27|P-value:5.33E-4|Clearance:0.05||SGD DESC:Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog Gene:FHL1(YPR104C)|FD-Score:3.58|P-value:1.72E-4|Clearance:0.31||SGD DESC:Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants Gene:NIC96(YFR002W)|FD-Score:3.18|P-value:7.47E-4|Clearance:0.04||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:POP4(YBR257W)|FD-Score:4.1|P-value:2.02E-5|Clearance:0.32||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:PRP40(YKL012W)|FD-Score:3.78|P-value:7.73E-5|Clearance:0.14||SGD DESC:U1 snRNP protein involved in splicing, interacts with the branchpoint-binding protein during the formation of the second commitment complex Gene:RNR2(YJL026W)|FD-Score:5.4|P-value:3.24E-8|Clearance:0.96||SGD DESC:Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits Gene:RPB2(YOR151C)|FD-Score:3.12|P-value:9.00E-4|Clearance:0.05||SGD DESC:RNA polymerase II second largest subunit B150, part of central core; similar to bacterial beta subunit Gene:SEC39(YLR440C)|FD-Score:3.64|P-value:1.37E-4|Clearance:0.01||SGD DESC:Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope Gene:SNM1(YDR478W)|FD-Score:3.14|P-value:8.55E-4|Clearance:0.01||SGD DESC:Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP Gene:SPB1(YCL054W)|FD-Score:3.13|P-value:8.86E-4|Clearance:0||SGD DESC:AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants Gene:STU1(YBL034C)|FD-Score:-3.12|P-value:9.00E-4|Clearance:0||SGD DESC:Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles Gene:TAF9(YMR236W)|FD-Score:3.22|P-value:6.31E-4|Clearance:0.05||SGD DESC:Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3 Gene:YGL074C(YGL074C_d)|FD-Score:3.63|P-value:1.40E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor Gene:YPT1(YFL038C)|FD-Score:4.44|P-value:4.44E-6|Clearance:0.34||SGD DESC:Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p)

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5380509
Download HOP data (tab-delimited text)  (excel)
Gene:ADH6(YMR318C)|FD-Score:-3.49|P-value:2.45E-4||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress Gene:APM1(YPL259C)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting Gene:ATG17(YLR423C)|FD-Score:3.78|P-value:7.90E-5||SGD DESC:Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells Gene:ATG19(YOL082W)|FD-Score:-3.11|P-value:9.27E-4||SGD DESC:Receptor protein for the cytoplasm-to-vacuole targeting (Cvt) pathway; delivers cargo proteins aminopeptidase I (Ape1p) and alpha-mannosidase (Ams1p) to the phagophore assembly site for packaging into Cvt vesicles Gene:CPA1(YOR303W)|FD-Score:-3.29|P-value:5.05E-4||SGD DESC:Small subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor; translationally regulated by an attenuator peptide encoded by YOR302W within the CPA1 mRNA 5'-leader Gene:ENT1(YDL161W)|FD-Score:-3.2|P-value:6.95E-4||SGD DESC:Epsin-like protein involved in endocytosis and actin patch assembly; functionally redundant with Ent2p; binds clathrin via a clathrin-binding domain motif at C-terminus; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:HIR3(YJR140C)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Subunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein Gene:HOM6(YJR139C)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:IKI1(YHR187W)|FD-Score:3.11|P-value:9.24E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin Gene:MAM3(YOL060C)|FD-Score:3.13|P-value:8.61E-4||SGD DESC:Protein required for normal mitochondrial morphology, has similarity to hemolysins Gene:MUK1(YPL070W)|FD-Score:-4.44|P-value:4.49E-6||SGD DESC:Cytoplasmic protein of unknown function containing a Vps9 domain; computational analysis of large-scale protein-protein interaction data suggests a possible role in transcriptional regulation Gene:RPL41B(YDL133C-A)|FD-Score:3.13|P-value:8.86E-4||SGD DESC:Ribosomal 60S subunit protein L41B; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41B has a paralog, RPL41A, that arose from the whole genome duplication Gene:RPL6B(YLR448W)|FD-Score:4.43|P-value:4.62E-6||SGD DESC:Ribosomal 60S subunit protein L6B; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6B has a paralog, RPL6A, that arose from the whole genome duplication Gene:SWS2(YNL081C)|FD-Score:3.74|P-value:9.14E-5||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TAE1(YBR261C)|FD-Score:-3.2|P-value:6.80E-4||SGD DESC:AdoMet-dependent proline methyltransferase; catalyzes the dimethylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues; has a role in protein synthesis; fusion protein localizes to the cytoplasm Gene:TAT2(YOL020W)|FD-Score:3.2|P-value:6.86E-4||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:YBR071W(YBR071W)|FD-Score:-3.53|P-value:2.05E-4||SGD DESC:Protein of unknown function found in the cytoplasm and bud neck; mRNA expression may be regulated by the cell cycle and/or cell wall stress; overexpression of YBR071W affects endocytic protein trafficking Gene:YFR056C(YFR056C_d)|FD-Score:4.46|P-value:4.02E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YFR055W Gene:YGL108C(YGL108C_p)|FD-Score:-3.77|P-value:8.17E-5||SGD DESC:Protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress Gene:YJL152W(YJL152W_d)|FD-Score:-3.61|P-value:1.55E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLL053C(YLL053C_p)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:Putative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin Gene:YLL054C(YLL054C_p)|FD-Score:4.05|P-value:2.51E-5||SGD DESC:Putative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene Gene:YLR407W(YLR407W_p)|FD-Score:3.18|P-value:7.33E-4||SGD DESC:Putative protein of unknown function; null mutant displays elongated buds and a large fraction of budded cells have only one nucleus Gene:YNL193W(YNL193W_p)|FD-Score:-3.38|P-value:3.63E-4||SGD DESC:Putative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis Gene:YOL046C(YOL046C_d)|FD-Score:4.88|P-value:5.28E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data;almost completely overlaps the verified gene PSK2/YOL045W Gene:YOR223W(YOR223W)|FD-Score:4.54|P-value:2.83E-6||SGD DESC:Subunit of the DSC ubiquitin ligase complex; protein of unknown function that localizes to the ER and vacuole lumen; overexpression affects endocytic protein trafficking; ortholog of fission yeast dsc3 Gene:YOR228C(YOR228C)|FD-Score:-3.17|P-value:7.58E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YOR318C(YOR318C_d)|FD-Score:3.11|P-value:9.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo Gene:YPK1(YKL126W)|FD-Score:4.45|P-value:4.32E-6||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:ADH6(YMR318C)|FD-Score:-3.49|P-value:2.45E-4||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress Gene:APM1(YPL259C)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting Gene:ATG17(YLR423C)|FD-Score:3.78|P-value:7.90E-5||SGD DESC:Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells Gene:ATG19(YOL082W)|FD-Score:-3.11|P-value:9.27E-4||SGD DESC:Receptor protein for the cytoplasm-to-vacuole targeting (Cvt) pathway; delivers cargo proteins aminopeptidase I (Ape1p) and alpha-mannosidase (Ams1p) to the phagophore assembly site for packaging into Cvt vesicles Gene:CPA1(YOR303W)|FD-Score:-3.29|P-value:5.05E-4||SGD DESC:Small subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor; translationally regulated by an attenuator peptide encoded by YOR302W within the CPA1 mRNA 5'-leader Gene:ENT1(YDL161W)|FD-Score:-3.2|P-value:6.95E-4||SGD DESC:Epsin-like protein involved in endocytosis and actin patch assembly; functionally redundant with Ent2p; binds clathrin via a clathrin-binding domain motif at C-terminus; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:HIR3(YJR140C)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Subunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein Gene:HOM6(YJR139C)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:IKI1(YHR187W)|FD-Score:3.11|P-value:9.24E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin Gene:MAM3(YOL060C)|FD-Score:3.13|P-value:8.61E-4||SGD DESC:Protein required for normal mitochondrial morphology, has similarity to hemolysins Gene:MUK1(YPL070W)|FD-Score:-4.44|P-value:4.49E-6||SGD DESC:Cytoplasmic protein of unknown function containing a Vps9 domain; computational analysis of large-scale protein-protein interaction data suggests a possible role in transcriptional regulation Gene:RPL41B(YDL133C-A)|FD-Score:3.13|P-value:8.86E-4||SGD DESC:Ribosomal 60S subunit protein L41B; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41B has a paralog, RPL41A, that arose from the whole genome duplication Gene:RPL6B(YLR448W)|FD-Score:4.43|P-value:4.62E-6||SGD DESC:Ribosomal 60S subunit protein L6B; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6B has a paralog, RPL6A, that arose from the whole genome duplication Gene:SWS2(YNL081C)|FD-Score:3.74|P-value:9.14E-5||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TAE1(YBR261C)|FD-Score:-3.2|P-value:6.80E-4||SGD DESC:AdoMet-dependent proline methyltransferase; catalyzes the dimethylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues; has a role in protein synthesis; fusion protein localizes to the cytoplasm Gene:TAT2(YOL020W)|FD-Score:3.2|P-value:6.86E-4||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:YBR071W(YBR071W)|FD-Score:-3.53|P-value:2.05E-4||SGD DESC:Protein of unknown function found in the cytoplasm and bud neck; mRNA expression may be regulated by the cell cycle and/or cell wall stress; overexpression of YBR071W affects endocytic protein trafficking Gene:YFR056C(YFR056C_d)|FD-Score:4.46|P-value:4.02E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YFR055W Gene:YGL108C(YGL108C_p)|FD-Score:-3.77|P-value:8.17E-5||SGD DESC:Protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress Gene:YJL152W(YJL152W_d)|FD-Score:-3.61|P-value:1.55E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLL053C(YLL053C_p)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:Putative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin Gene:YLL054C(YLL054C_p)|FD-Score:4.05|P-value:2.51E-5||SGD DESC:Putative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene Gene:YLR407W(YLR407W_p)|FD-Score:3.18|P-value:7.33E-4||SGD DESC:Putative protein of unknown function; null mutant displays elongated buds and a large fraction of budded cells have only one nucleus Gene:YNL193W(YNL193W_p)|FD-Score:-3.38|P-value:3.63E-4||SGD DESC:Putative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis Gene:YOL046C(YOL046C_d)|FD-Score:4.88|P-value:5.28E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data;almost completely overlaps the verified gene PSK2/YOL045W Gene:YOR223W(YOR223W)|FD-Score:4.54|P-value:2.83E-6||SGD DESC:Subunit of the DSC ubiquitin ligase complex; protein of unknown function that localizes to the ER and vacuole lumen; overexpression affects endocytic protein trafficking; ortholog of fission yeast dsc3 Gene:YOR228C(YOR228C)|FD-Score:-3.17|P-value:7.58E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YOR318C(YOR318C_d)|FD-Score:3.11|P-value:9.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo Gene:YPK1(YKL126W)|FD-Score:4.45|P-value:4.32E-6||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJL026W5.403.24E-80.96RNR2Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits
YFL038C4.444.44E-60.34YPT1Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p)
YBR257W4.102.02E-50.32POP4Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
YKL012W3.787.73E-50.14PRP40U1 snRNP protein involved in splicing, interacts with the branchpoint-binding protein during the formation of the second commitment complex
YLR440C3.641.37E-40.01SEC39Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope
YGL074C_d3.631.40E-40.05YGL074C_dDubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor
YPR104C3.581.72E-40.31FHL1Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants
YOR335C3.275.33E-40.05ALA1Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog
YMR236W3.226.31E-40.05TAF9Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3
YFR002W3.187.47E-40.04NIC96Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p)
YDR478W3.148.55E-40.01SNM1Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP
YCL054W3.138.86E-40.00SPB1AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants
YOR151C3.129.00E-40.05RPB2RNA polymerase II second largest subunit B150, part of central core; similar to bacterial beta subunit
YHR107C3.080.001050.07CDC12Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YLR317W_d3.010.001300.18YLR317W_dDubious open reading frame; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOL046C_d4.885.28E-7YOL046C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data;almost completely overlaps the verified gene PSK2/YOL045W
YOR223W4.542.83E-6YOR223WSubunit of the DSC ubiquitin ligase complex; protein of unknown function that localizes to the ER and vacuole lumen; overexpression affects endocytic protein trafficking; ortholog of fission yeast dsc3
YFR056C_d4.464.02E-6YFR056C_dDubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YFR055W
YKL126W4.454.32E-6YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YLR448W4.434.62E-6RPL6BRibosomal 60S subunit protein L6B; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6B has a paralog, RPL6A, that arose from the whole genome duplication
YLL053C_p4.251.06E-5YLL053C_pPutative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin
YLL054C_p4.052.51E-5YLL054C_pPutative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene
YLR423C3.787.90E-5ATG17Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells
YNL081C3.749.14E-5SWS2Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency
YJR139C3.661.25E-4HOM6Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions
YJR140C3.571.77E-4HIR3Subunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein
YPL259C3.413.27E-4APM1Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting
YOL020W3.206.86E-4TAT2High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance
YLR407W_p3.187.33E-4YLR407W_pPutative protein of unknown function; null mutant displays elongated buds and a large fraction of budded cells have only one nucleus
YOL060C3.138.61E-4MAM3Protein required for normal mitochondrial morphology, has similarity to hemolysins

GO enrichment analysis for SGTC_303
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1071.27E-16SGTC_14113909-8223 150.0 μMChemDiv (Drug-like library)X14110.0843373
0.0993.28E-14SGTC_2413inermin 26.1 μMTimTec (Natural product derivative library)915100.0895522
0.0903.69E-12SGTC_2230bromochlorosalicylanilide 954.5 nMMiscellaneous772540.25
0.0849.58E-11SGTC_7243910-0327 137.0 μMChemDiv (Drug-like library)28854830.0972222ERG2
0.0841.31E-10SGTC_21565649887 115.0 μMChembridge (Fragment library)54680440NARPP1 & pyrimidine depletion
0.0813.63E-10SGTC_2732terfenadine 20.0 μMMiscellaneous54050.0789474ERG2
0.0765.33E-9SGTC_23399000433 200.0 μMChembridge (Fragment library)29889540.0793651
0.0741.30E-8SGTC_1748st037455 53.6 μMTimTec (Natural product derivative library)5806900.0821918
0.0714.95E-8SGTC_492tmb-8 126.0 μMICCB bioactive library54940.0416667
0.0699.63E-8SGTC_29879018338 71.4 μMChembridge (Drug-like library)50556820.0945946RPP1 & pyrimidine depletion
0.0691.01E-7SGTC_14504112-4260 37.6 μMChemDiv (Drug-like library)16331750.121622calcium & mitochondrial duress
0.0663.25E-7SGTC_400oxethazaine 37.5 μMMiscellaneous46210.0895522calcium & mitochondrial duress
0.0664.15E-7SGTC_1464k208-0027 110.0 μMChemDiv (Drug-like library)X14640.148148
0.0664.63E-7SGTC_20575236571 38.3 μMChembridge (Fragment library)54684039NARPP1 & pyrimidine depletion
0.0655.72E-7SGTC_810411-0046 10.0 μMChemDiv (Drug-like library)129252650.0645161

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3191348-159645.5 μM0.75380505ChemDiv (Drug-like library)213.235241.1624RSC & ERG11
SGTC_1571348-169565.82 μM0.6363645380510ChemDiv (Drug-like library)229.234640.62335iron homeostasis
SGTC_4260988-003714.6 μM0.3962265383008ChemDiv (Drug-like library)257.244741.51425ERAD & cell cycle
SGTC_12500485-034940.2 μM0.3921576842794ChemDiv (Drug-like library)334.25082.95412
SGTC_1320417-171525.81 μM0.389836769098ChemDiv (Drug-like library)409.299024.56114RPP1 & pyrimidine depletion
SGTC_3301488-031980.1 μM0.389836846614ChemDiv (Drug-like library)392.248663.99223RPP1 & pyrimidine depletion
SGTC_9611252-1571372 μM0.3846156813498ChemDiv (Drug-like library)288.16421.05124
SGTC_910417-175158.38 μM0.3770496795624ChemDiv (Drug-like library)409.299024.56114Golgi
SGTC_13221348-107012 μM0.3709686758089ChemDiv (Drug-like library)402.308143.54224
SGTC_185k048-003725.49 μM0.3684216827589ChemDiv (Drug-like library)414.04882.30735RSC & ERG11