9090421

methyl 5-propan-2-yl-2-(pyridine-3-carbonylamino)thiophene-3-carboxylate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3034
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 17173761
SMILES CC(C)C1=CC(=C(S1)NC(=O)C2=CN=CC=C2)C(=O)OC
Standardized SMILES COC(=O)c1cc(sc1NC(=O)c2cccnc2)C(C)C
Molecular weight 304.3641
ALogP 2.38
H-bond donor count 1
H-bond acceptor count 5
Response signature 60S ribosome export

Pool Growth Kinetics
% growth inhibition (Het. pool) 10.62
% growth inhibition (Hom. pool) 9.94


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 17173761
Download HIP data (tab-delimited text)  (excel)
Gene:CDC5(YMR001C)|FD-Score:6.24|P-value:2.16E-10|Clearance:2.47||SGD DESC:Polo-like kinase with multiple functions in mitosis and cytokinesis through substrate phosphorylation, also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate Gene:HSP10(YOR020C)|FD-Score:3.32|P-value:4.52E-4|Clearance:0.22||SGD DESC:Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES Gene:LSG1(YGL099W)|FD-Score:6.31|P-value:1.37E-10|Clearance:2.47||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MYO1(YHR023W)|FD-Score:3.77|P-value:8.20E-5|Clearance:0.03||SGD DESC:Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively Gene:NSA2(YER126C)|FD-Score:3.74|P-value:9.18E-5|Clearance:0.23||SGD DESC:Protein constituent of 66S pre-ribosomal particles, contributes to processing of the 27S pre-rRNA Gene:PGI1(YBR196C)|FD-Score:3.1|P-value:9.67E-4|Clearance:0.02||SGD DESC:Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation Gene:RPB10(YOR210W)|FD-Score:-3.73|P-value:9.55E-5|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:SEC6(YIL068C)|FD-Score:-3.22|P-value:6.44E-4|Clearance:0||SGD DESC:Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p Gene:YGR114C(YGR114C_d)|FD-Score:3.51|P-value:2.26E-4|Clearance:0.19||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:CDC5(YMR001C)|FD-Score:6.24|P-value:2.16E-10|Clearance:2.47||SGD DESC:Polo-like kinase with multiple functions in mitosis and cytokinesis through substrate phosphorylation, also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate Gene:HSP10(YOR020C)|FD-Score:3.32|P-value:4.52E-4|Clearance:0.22||SGD DESC:Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES Gene:LSG1(YGL099W)|FD-Score:6.31|P-value:1.37E-10|Clearance:2.47||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MYO1(YHR023W)|FD-Score:3.77|P-value:8.20E-5|Clearance:0.03||SGD DESC:Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively Gene:NSA2(YER126C)|FD-Score:3.74|P-value:9.18E-5|Clearance:0.23||SGD DESC:Protein constituent of 66S pre-ribosomal particles, contributes to processing of the 27S pre-rRNA Gene:PGI1(YBR196C)|FD-Score:3.1|P-value:9.67E-4|Clearance:0.02||SGD DESC:Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation Gene:RPB10(YOR210W)|FD-Score:-3.73|P-value:9.55E-5|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:SEC6(YIL068C)|FD-Score:-3.22|P-value:6.44E-4|Clearance:0||SGD DESC:Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p Gene:YGR114C(YGR114C_d)|FD-Score:3.51|P-value:2.26E-4|Clearance:0.19||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 17173761
Download HOP data (tab-delimited text)  (excel)
Gene:AIM46(YHR199C)|FD-Score:3.22|P-value:6.35E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ARC18(YLR370C)|FD-Score:4.89|P-value:4.94E-7||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARO3(YDR035W)|FD-Score:-3.15|P-value:8.25E-4||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:CHO2(YGR157W)|FD-Score:-3.69|P-value:1.10E-4||SGD DESC:Phosphatidylethanolamine methyltransferase (PEMT), catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis Gene:CWC21(YDR482C)|FD-Score:-3.25|P-value:5.87E-4||SGD DESC:Protein involved in RNA splicing by the spliceosome; component of a complex containing Cef1p; interacts genetically with ISY1 and BUD13; may bind RNA; has similarity to S. pombe Cwf21p Gene:DAL81(YIR023W)|FD-Score:6.89|P-value:2.80E-12||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:GAL83(YER027C)|FD-Score:3.18|P-value:7.41E-4||SGD DESC:One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain Gene:HIR3(YJR140C)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Subunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein Gene:IME4(YGL192W)|FD-Score:-3.47|P-value:2.61E-4||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:INM1(YHR046C)|FD-Score:4.7|P-value:1.28E-6||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate Gene:IRS4(YKR019C)|FD-Score:5.61|P-value:9.95E-9||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KCH1(YJR054W_p)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate Gene:LRP1(YHR081W)|FD-Score:4.1|P-value:2.06E-5||SGD DESC:Nuclear exosome-associated nucleic acid binding protein; involved in RNA processing, surveillance, degradation, tethering, and export; homolog of mammalian nuclear matrix protein C1D involved in regulation of DNA repair and recombination Gene:MAC1(YMR021C)|FD-Score:-4.29|P-value:8.86E-6||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MPP6(YNR024W)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes Gene:OPI9(YLR338W_d)|FD-Score:7.21|P-value:2.84E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:RIM9(YMR063W)|FD-Score:-3.89|P-value:5.02E-5||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RPL22A(YLR061W)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RTN2(YDL204W)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication Gene:SAC6(YDR129C)|FD-Score:4.29|P-value:8.77E-6||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SEC22(YLR268W)|FD-Score:-3.81|P-value:6.94E-5||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SLG1(YOR008C)|FD-Score:3.2|P-value:6.98E-4||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SMY2(YBR172C)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Protein of unknown function involved in COPII vesicle formation; interacts with the Sec23p/Sec24p subcomplex; overexpression suppresses the temperature sensitivity of a myo2 mutant; similar to S. pombe Mpd2; SMY2 has a paralog, SYH1, that arose from the whole genome duplication Gene:SNF12(YNR023W)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive Gene:SPC2(YML055W)|FD-Score:-3.28|P-value:5.19E-4||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC25 Gene:TMA23(YMR269W)|FD-Score:4.29|P-value:8.85E-6||SGD DESC:Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan Gene:TRP2(YER090W)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:VAC17(YCL063W)|FD-Score:-3.16|P-value:8.00E-4||SGD DESC:Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p Gene:VAM10(YOR068C)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering Gene:VID28(YIL017C)|FD-Score:-3.49|P-value:2.45E-4||SGD DESC:GID Complex subunit, serves as adaptor for regulatory subunit Vid24p; protein involved in proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase); localized to the nucleus and the cytoplasm Gene:VNX1(YNL321W)|FD-Score:3.84|P-value:6.25E-5||SGD DESC:Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate Gene:VTC2(YFL004W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion Gene:YAL066W(YAL066W_d)|FD-Score:-3.59|P-value:1.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL070C(YBL070C_d)|FD-Score:-3.45|P-value:2.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR027C(YBR027C_d)|FD-Score:-3.09|P-value:9.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR050C(YCR050C_p)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein Gene:YER046W-A(YER046W-A_d)|FD-Score:3.15|P-value:8.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER121W(YER121W_p)|FD-Score:-3.47|P-value:2.56E-4||SGD DESC:Putative protein of unknown function; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy Gene:YLL007C(YLL007C_p)|FD-Score:-3.19|P-value:7.00E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YMR320W(YMR320W_d)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL319W(YNL319W_d)|FD-Score:-3.99|P-value:3.25E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HXT14 Gene:YOR131C(YOR131C_p)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:Putative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress Gene:AIM46(YHR199C)|FD-Score:3.22|P-value:6.35E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ARC18(YLR370C)|FD-Score:4.89|P-value:4.94E-7||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARO3(YDR035W)|FD-Score:-3.15|P-value:8.25E-4||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:CHO2(YGR157W)|FD-Score:-3.69|P-value:1.10E-4||SGD DESC:Phosphatidylethanolamine methyltransferase (PEMT), catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis Gene:CWC21(YDR482C)|FD-Score:-3.25|P-value:5.87E-4||SGD DESC:Protein involved in RNA splicing by the spliceosome; component of a complex containing Cef1p; interacts genetically with ISY1 and BUD13; may bind RNA; has similarity to S. pombe Cwf21p Gene:DAL81(YIR023W)|FD-Score:6.89|P-value:2.80E-12||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:GAL83(YER027C)|FD-Score:3.18|P-value:7.41E-4||SGD DESC:One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain Gene:HIR3(YJR140C)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Subunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein Gene:IME4(YGL192W)|FD-Score:-3.47|P-value:2.61E-4||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:INM1(YHR046C)|FD-Score:4.7|P-value:1.28E-6||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate Gene:IRS4(YKR019C)|FD-Score:5.61|P-value:9.95E-9||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KCH1(YJR054W_p)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate Gene:LRP1(YHR081W)|FD-Score:4.1|P-value:2.06E-5||SGD DESC:Nuclear exosome-associated nucleic acid binding protein; involved in RNA processing, surveillance, degradation, tethering, and export; homolog of mammalian nuclear matrix protein C1D involved in regulation of DNA repair and recombination Gene:MAC1(YMR021C)|FD-Score:-4.29|P-value:8.86E-6||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MPP6(YNR024W)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes Gene:OPI9(YLR338W_d)|FD-Score:7.21|P-value:2.84E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:RIM9(YMR063W)|FD-Score:-3.89|P-value:5.02E-5||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RPL22A(YLR061W)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RTN2(YDL204W)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication Gene:SAC6(YDR129C)|FD-Score:4.29|P-value:8.77E-6||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SEC22(YLR268W)|FD-Score:-3.81|P-value:6.94E-5||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SLG1(YOR008C)|FD-Score:3.2|P-value:6.98E-4||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SMY2(YBR172C)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Protein of unknown function involved in COPII vesicle formation; interacts with the Sec23p/Sec24p subcomplex; overexpression suppresses the temperature sensitivity of a myo2 mutant; similar to S. pombe Mpd2; SMY2 has a paralog, SYH1, that arose from the whole genome duplication Gene:SNF12(YNR023W)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive Gene:SPC2(YML055W)|FD-Score:-3.28|P-value:5.19E-4||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC25 Gene:TMA23(YMR269W)|FD-Score:4.29|P-value:8.85E-6||SGD DESC:Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan Gene:TRP2(YER090W)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:VAC17(YCL063W)|FD-Score:-3.16|P-value:8.00E-4||SGD DESC:Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p Gene:VAM10(YOR068C)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering Gene:VID28(YIL017C)|FD-Score:-3.49|P-value:2.45E-4||SGD DESC:GID Complex subunit, serves as adaptor for regulatory subunit Vid24p; protein involved in proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase); localized to the nucleus and the cytoplasm Gene:VNX1(YNL321W)|FD-Score:3.84|P-value:6.25E-5||SGD DESC:Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate Gene:VTC2(YFL004W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion Gene:YAL066W(YAL066W_d)|FD-Score:-3.59|P-value:1.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL070C(YBL070C_d)|FD-Score:-3.45|P-value:2.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR027C(YBR027C_d)|FD-Score:-3.09|P-value:9.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR050C(YCR050C_p)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein Gene:YER046W-A(YER046W-A_d)|FD-Score:3.15|P-value:8.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER121W(YER121W_p)|FD-Score:-3.47|P-value:2.56E-4||SGD DESC:Putative protein of unknown function; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy Gene:YLL007C(YLL007C_p)|FD-Score:-3.19|P-value:7.00E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YMR320W(YMR320W_d)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL319W(YNL319W_d)|FD-Score:-3.99|P-value:3.25E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HXT14 Gene:YOR131C(YOR131C_p)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:Putative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL099W6.311.37E-102.47LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YMR001C6.242.16E-102.47CDC5Polo-like kinase with multiple functions in mitosis and cytokinesis through substrate phosphorylation, also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate
YHR023W3.778.20E-50.03MYO1Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively
YER126C3.749.18E-50.23NSA2Protein constituent of 66S pre-ribosomal particles, contributes to processing of the 27S pre-rRNA
YGR114C_d3.512.26E-40.19YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YOR020C3.324.52E-40.22HSP10Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES
YBR196C3.109.67E-40.02PGI1Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation
YJL167W3.080.001040.03ERG20Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis
YLR347C3.050.001160.09KAP95Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p
YLR359W2.950.001570.01ADE13Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency
YIR015W2.940.001640.06RPR2Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress
YBR049C2.880.001990.25REB1RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication
YNL161W2.630.004230.15CBK1Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress
YPL124W2.480.006520.06SPC29Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication
YDL195W2.430.007630.10SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR338W_d7.212.84E-13OPI9_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C
YIR023W6.892.80E-12DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YKR019C5.619.95E-9IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YLR370C4.894.94E-7ARC18Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YHR046C4.701.28E-6INM1Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate
YDR129C4.298.77E-6SAC6Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress
YMR269W4.298.85E-6TMA23Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan
YHR081W4.102.06E-5LRP1Nuclear exosome-associated nucleic acid binding protein; involved in RNA processing, surveillance, degradation, tethering, and export; homolog of mammalian nuclear matrix protein C1D involved in regulation of DNA repair and recombination
YNL321W3.846.25E-5VNX1Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate
YFL004W3.701.07E-4VTC2Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion
YDL204W3.542.02E-4RTN2Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication
YMR320W_d3.452.80E-4YMR320W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YJR054W_p3.442.89E-4KCH1_pPotassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
YOR068C3.383.66E-4VAM10Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering
YLR061W3.344.17E-4RPL22ARibosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication

GO enrichment analysis for SGTC_3034
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2078.85E-58SGTC_24605552655 174.2 μMMiscellaneous54019890.157143amide catabolism
0.1991.77E-53SGTC_9903985-0011 41.3 μMChemDiv (Drug-like library)53995650.106667amide catabolism
0.1702.89E-39SGTC_31689103419 49.5 μMChembridge (Drug-like library)166492560.20270360S ribosome export
0.1505.36E-31SGTC_10583448-5425 97.3 μMChemDiv (Drug-like library)72797050.1
0.1491.12E-30SGTC_1958st076513 41.9 μMTimTec (Natural product derivative library)7321220.0860S ribosome export
0.1491.84E-30SGTC_486niguldipine 82.0 μMMiscellaneous12360.14433amide catabolism
0.1475.76E-30SGTC_28459003024 43.0 μMChembridge (Drug-like library)29901730.2amide catabolism
0.1412.36E-27SGTC_3371315-0038 354.0 μMChemDiv (Drug-like library)16264370.091954amide catabolism
0.1375.26E-26SGTC_5694130-2812 160.0 μMChemDiv (Drug-like library)7435520.0869565amide catabolism
0.1322.86E-24SGTC_12670828-0272 9.5 μMChemDiv (Drug-like library)7453340.128571
0.1271.15E-22SGTC_32259130819 49.5 μMChembridge (Drug-like library)45471210.175676amide catabolism
0.1232.57E-21SGTC_32379133362 49.5 μMChembridge (Drug-like library)438451370.182927
0.1224.66E-21SGTC_2680butyl paraben 22.2 μMMicrosource (Natural product library)71840.15873amide catabolism
0.1211.66E-20SGTC_31749107204 49.5 μMChembridge (Drug-like library)39388020.108434ERG2
0.1203.11E-20SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.16901460S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3032908942749.47 μM0.78846217173707Chembridge (Drug-like library)318.390722.7291560S ribosome export
SGTC_2854902455971.43 μM0.4769233163529Chembridge (Drug-like library)341.361161.16916
SGTC_22857945142200 μM0.4642862970608Chembridge (Fragment library)247.273060.25224
SGTC_3025908789549.47 μM0.42424217173641Chembridge (Drug-like library)365.448762.88924PDR1
SGTC_3022908799349.47 μM0.42187517173644Chembridge (Drug-like library)351.422182.40224
SGTC_9861493-048384 μM0.41071440079ChemDiv (Drug-like library)256.256681.31914
SGTC_3151909982149.47 μM0.40298517174189Chembridge (Drug-like library)329.416662.03624
SGTC_22199098279200 μM0.388889902349Chembridge (Fragment library)213.253540.73714
SGTC_2967908953849.85 μM0.370968728216Chembridge (Drug-like library)300.663633.0715
SGTC_1759st04305970 μM0.366667669426TimTec (Natural product derivative library)285.29462.45314amide catabolism