9090181

(4,5-dimethylthiophen-3-yl)-[4-(2-methylphenyl)piperazin-1-yl]methanone

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3036
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 25236586
SMILES CC1=CC=CC=C1N2CCN(CC2)C(=O)C3=CSC(=C3C)C
Standardized SMILES Cc1ccccc1N2CCN(CC2)C(=O)c3csc(C)c3C
Molecular weight 314.4451
ALogP 3.65
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.77
% growth inhibition (Hom. pool) 7.78


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 25236586
Download HIP data (tab-delimited text)  (excel)
Gene:DBF4(YDR052C)|FD-Score:4.19|P-value:1.38E-5|Clearance:0.47||SGD DESC:Regulatory subunit of Cdc7p-Dbf4p kinase complex; required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; relative distribution to the nucleus increases upon DNA replication stress Gene:ERG12(YMR208W)|FD-Score:3.73|P-value:9.73E-5|Clearance:0.06||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:MCD4(YKL165C)|FD-Score:5.45|P-value:2.55E-8|Clearance:0.83||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:PDS5(YMR076C)|FD-Score:3.56|P-value:1.88E-4|Clearance:0.03||SGD DESC:Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis Gene:RIB5(YBR256C)|FD-Score:5.02|P-value:2.61E-7|Clearance:0.83||SGD DESC:Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway Gene:RLI1(YDR091C)|FD-Score:3.52|P-value:2.12E-4|Clearance:0.48||SGD DESC:Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species Gene:RPR2(YIR015W)|FD-Score:3.67|P-value:1.24E-4|Clearance:0.11||SGD DESC:Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress Gene:SLD3(YGL113W)|FD-Score:-3.68|P-value:1.19E-4|Clearance:0||SGD DESC:Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:DBF4(YDR052C)|FD-Score:4.19|P-value:1.38E-5|Clearance:0.47||SGD DESC:Regulatory subunit of Cdc7p-Dbf4p kinase complex; required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; relative distribution to the nucleus increases upon DNA replication stress Gene:ERG12(YMR208W)|FD-Score:3.73|P-value:9.73E-5|Clearance:0.06||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:MCD4(YKL165C)|FD-Score:5.45|P-value:2.55E-8|Clearance:0.83||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:PDS5(YMR076C)|FD-Score:3.56|P-value:1.88E-4|Clearance:0.03||SGD DESC:Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis Gene:RIB5(YBR256C)|FD-Score:5.02|P-value:2.61E-7|Clearance:0.83||SGD DESC:Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway Gene:RLI1(YDR091C)|FD-Score:3.52|P-value:2.12E-4|Clearance:0.48||SGD DESC:Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species Gene:RPR2(YIR015W)|FD-Score:3.67|P-value:1.24E-4|Clearance:0.11||SGD DESC:Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress Gene:SLD3(YGL113W)|FD-Score:-3.68|P-value:1.19E-4|Clearance:0||SGD DESC:Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 25236586
Download HOP data (tab-delimited text)  (excel)
Gene:ABP1(YCR088W)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Actin-binding protein of the cortical actin cytoskeleton, important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization; phosphorylation within its PRR (Proline-Rich Region), mediated by Cdc28p and Pho85p, protects Abp1p from proteolysis mediated by its own PEST sequences Gene:GAL83(YER027C)|FD-Score:-3.21|P-value:6.58E-4||SGD DESC:One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain Gene:ISA2(YPR067W)|FD-Score:4.58|P-value:2.33E-6||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations Gene:ISW1(YBR245C)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p (Isw1a), and a complex with Ioc2p and Ioc4p (Isw1b); Isw1a and Isw1b have partially overlapping and distinct roles, Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation; Isw1b recruited to open reading frames by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions Gene:MMT1(YMR177W)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt2p Gene:MRPL13(YKR006C)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MTO1(YGL236C)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Mitochondrial protein, forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants Gene:PAC2(YER007W)|FD-Score:4.45|P-value:4.32E-6||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PMR1(YGL167C)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease Gene:ROX3(YBL093C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme Gene:RPL33B(YOR234C)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Ribosomal 60S subunit protein L33B; rpl33b null mutant exhibits normal growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33B has a paralog, RPL33A, that arose from the whole genome duplication Gene:SHB17(YKR043C)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SPF1(YEL031W)|FD-Score:-3.22|P-value:6.44E-4||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPR1(YOR190W)|FD-Score:3.21|P-value:6.73E-4||SGD DESC:Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance Gene:SSD1(YDR293C)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:TEF4(YKL081W)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:Gamma subunit of translational elongation factor eEF1B, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex Gene:TEL1(YBL088C)|FD-Score:5.14|P-value:1.39E-7||SGD DESC:Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585) Gene:UBA3(YPR066W)|FD-Score:5.38|P-value:3.76E-8||SGD DESC:Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:VMA4(YOR332W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress Gene:YAP3(YHL009C)|FD-Score:-4.18|P-value:1.49E-5||SGD DESC:Basic leucine zipper (bZIP) transcription factor Gene:YEL057C(YEL057C_p)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Protein of unknown function involved in telomere maintenance; target of UME6 regulation Gene:YIG1(YPL201C)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol Gene:YNR066C(YNR066C_p)|FD-Score:4.99|P-value:3.09E-7||SGD DESC:Putative membrane-localized protein of unknown function Gene:YOR131C(YOR131C_p)|FD-Score:-3.4|P-value:3.40E-4||SGD DESC:Putative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress Gene:ABP1(YCR088W)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Actin-binding protein of the cortical actin cytoskeleton, important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization; phosphorylation within its PRR (Proline-Rich Region), mediated by Cdc28p and Pho85p, protects Abp1p from proteolysis mediated by its own PEST sequences Gene:GAL83(YER027C)|FD-Score:-3.21|P-value:6.58E-4||SGD DESC:One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain Gene:ISA2(YPR067W)|FD-Score:4.58|P-value:2.33E-6||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations Gene:ISW1(YBR245C)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p (Isw1a), and a complex with Ioc2p and Ioc4p (Isw1b); Isw1a and Isw1b have partially overlapping and distinct roles, Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation; Isw1b recruited to open reading frames by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions Gene:MMT1(YMR177W)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt2p Gene:MRPL13(YKR006C)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MTO1(YGL236C)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Mitochondrial protein, forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants Gene:PAC2(YER007W)|FD-Score:4.45|P-value:4.32E-6||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PMR1(YGL167C)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease Gene:ROX3(YBL093C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme Gene:RPL33B(YOR234C)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Ribosomal 60S subunit protein L33B; rpl33b null mutant exhibits normal growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33B has a paralog, RPL33A, that arose from the whole genome duplication Gene:SHB17(YKR043C)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SPF1(YEL031W)|FD-Score:-3.22|P-value:6.44E-4||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPR1(YOR190W)|FD-Score:3.21|P-value:6.73E-4||SGD DESC:Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance Gene:SSD1(YDR293C)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:TEF4(YKL081W)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:Gamma subunit of translational elongation factor eEF1B, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex Gene:TEL1(YBL088C)|FD-Score:5.14|P-value:1.39E-7||SGD DESC:Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585) Gene:UBA3(YPR066W)|FD-Score:5.38|P-value:3.76E-8||SGD DESC:Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:VMA4(YOR332W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress Gene:YAP3(YHL009C)|FD-Score:-4.18|P-value:1.49E-5||SGD DESC:Basic leucine zipper (bZIP) transcription factor Gene:YEL057C(YEL057C_p)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Protein of unknown function involved in telomere maintenance; target of UME6 regulation Gene:YIG1(YPL201C)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol Gene:YNR066C(YNR066C_p)|FD-Score:4.99|P-value:3.09E-7||SGD DESC:Putative membrane-localized protein of unknown function Gene:YOR131C(YOR131C_p)|FD-Score:-3.4|P-value:3.40E-4||SGD DESC:Putative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YKL165C5.452.55E-80.83MCD4Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes
YBR256C5.022.61E-70.83RIB5Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway
YDR052C4.191.38E-50.47DBF4Regulatory subunit of Cdc7p-Dbf4p kinase complex; required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; relative distribution to the nucleus increases upon DNA replication stress
YMR208W3.739.73E-50.06ERG12Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YIR015W3.671.24E-40.11RPR2Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress
YMR076C3.561.88E-40.03PDS5Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis
YDR091C3.522.12E-40.48RLI1Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species
YDL220C3.050.001150.01CDC13Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation
YGR060W3.040.001180.06ERG25C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol
YDR478W2.980.001450.04SNM1Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP
YPR105C2.940.001660.02COG4Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YBR123C2.910.001780.22TFC1One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63
YGR251W2.690.003540.06NOP19Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes
YGL068W2.640.004170.03MNP1Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth
YJL202C_d2.610.004590.04YJL202C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 3' end of essential PRP21 gene encoding a subunit of the SF3a splicing factor complex

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPR066W5.383.76E-8UBA3Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern
YBL088C5.141.39E-7TEL1Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585)
YNR066C_p4.993.09E-7YNR066C_pPutative membrane-localized protein of unknown function
YPR067W4.582.33E-6ISA2Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations
YER007W4.454.32E-6PAC2Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl
YPL201C4.102.03E-5YIG1Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol
YOR332W3.631.43E-4VMA4Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress
YEL057C_p3.631.44E-4YEL057C_pProtein of unknown function involved in telomere maintenance; target of UME6 regulation
YOR234C3.581.69E-4RPL33BRibosomal 60S subunit protein L33B; rpl33b null mutant exhibits normal growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33B has a paralog, RPL33A, that arose from the whole genome duplication
YKL081W3.532.04E-4TEF4Gamma subunit of translational elongation factor eEF1B, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex
YCR088W3.522.19E-4ABP1Actin-binding protein of the cortical actin cytoskeleton, important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization; phosphorylation within its PRR (Proline-Rich Region), mediated by Cdc28p and Pho85p, protects Abp1p from proteolysis mediated by its own PEST sequences
YBL093C3.512.21E-4ROX3Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme
YGL167C3.363.88E-4PMR1High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease
YKR043C3.304.76E-4SHB17Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus
YBR245C3.265.56E-4ISW1ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p (Isw1a), and a complex with Ioc2p and Ioc4p (Isw1b); Isw1a and Isw1b have partially overlapping and distinct roles, Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation; Isw1b recruited to open reading frames by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions

GO enrichment analysis for SGTC_3036
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1142.05E-18SGTC_229benomyl 22.9 μMMiscellaneous287800.123288tubulin folding & SWR complex
0.0971.02E-13SGTC_286k060-0067 22.9 μMChemDiv (Drug-like library)59850510.0921053tubulin folding & SWR complex
0.0937.12E-13SGTC_21255246583 200.0 μMChembridge (Fragment library)8352670.142857tubulin folding & SWR complex
0.0912.81E-12SGTC_30209089140 49.5 μMChembridge (Drug-like library)172178590.220588
0.0871.93E-11SGTC_13521496-0991 99.0 μMChemDiv (Drug-like library)30000390.133333tubulin folding & SWR complex
0.0831.96E-10SGTC_2123807-4682 38.8 μMChemDiv (Drug-like library)31255010.0933333tubulin folding & SWR complex
0.0824.48E-10SGTC_1460k062-0059 9.2 μMChemDiv (Drug-like library)13730850.203125Golgi
0.0822.65E-10SGTC_20985376439 882.5 nMChembridge (Fragment library)28441160.121212
0.0815.64E-10SGTC_23196033012 152.6 μMChembridge (Fragment library)8472460.183333tubulin folding & SWR complex
0.0741.71E-8SGTC_2103551-0445 56.8 μMChemDiv (Drug-like library)28651800.16
0.0731.59E-8SGTC_30239089636 49.5 μMChembridge (Drug-like library)172492410.235294
0.0732.26E-8SGTC_1459k035-0031 35.7 μMChemDiv (Drug-like library)38771280.0491803DNA intercalators
0.0723.80E-8SGTC_1493970-0822 46.1 μMChemDiv (Drug-like library)31142500.0779221tubulin folding & SWR complex
0.0705.98E-8SGTC_30749116018 49.5 μMChembridge (Drug-like library)170273030.253731RNA processing & uracil transport
0.0706.04E-8SGTC_21745790901 200.0 μMChembridge (Fragment library)7860200.196721tubulin folding & SWR complex

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3138909721649.47 μM0.5825237486Chembridge (Drug-like library)328.471663.39903
SGTC_3143908665849.47 μM0.57446825236626Chembridge (Drug-like library)300.41853.45803
SGTC_3149909785549.47 μM0.5510225236625Chembridge (Drug-like library)365.287983.88103Golgi
SGTC_3029908868349.47 μM0.50980425236615Chembridge (Drug-like library)300.41853.34703
SGTC_22016605497169.86 μM0.478261670741Chembridge (Fragment library)232.32142.18102ergosterol biosynthesis
SGTC_3046909221249.47 μM0.43636425236637Chembridge (Drug-like library)328.3806433.404
SGTC_3156909993149.47 μM0.43636418111053Chembridge (Drug-like library)344.835243.85903
SGTC_3056909490049.47 μM0.42857125236784Chembridge (Drug-like library)342.4072233.88404
SGTC_3043909230349.47 μM0.42307725236084Chembridge (Drug-like library)299.430443.97302
SGTC_22267298446200 μM0.3928573283010Chembridge (Fragment library)287.379982.70104