9091290

(4-methyl-5-phenylthiophen-2-yl)-(2-methylpiperidin-1-yl)methanone

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3038
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 25236843
SMILES CC1CCCCN1C(=O)C2=CC(=C(S2)C3=CC=CC=C3)C
Standardized SMILES CC1CCCCN1C(=O)c2cc(C)c(s2)c3ccccc3
Molecular weight 299.4304
ALogP 4.79
H-bond donor count 0
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.33
% growth inhibition (Hom. pool) 8.86


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 25236843
Download HIP data (tab-delimited text)  (excel)
Gene:GCD1(YOR260W)|FD-Score:3.39|P-value:3.43E-4|Clearance:0.16||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:LSG1(YGL099W)|FD-Score:4.12|P-value:1.90E-5|Clearance:0.58||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MCM3(YEL032W)|FD-Score:-4.18|P-value:1.45E-5|Clearance:0||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex Gene:MPS2(YGL075C)|FD-Score:3.24|P-value:6.08E-4|Clearance:0.13||SGD DESC:Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p Gene:NFS1(YCL017C)|FD-Score:-3.64|P-value:1.36E-4|Clearance:0||SGD DESC:Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria Gene:NMD3(YHR170W)|FD-Score:3.54|P-value:2.00E-4|Clearance:0.14||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:PUP1(YOR157C)|FD-Score:-3.3|P-value:4.86E-4|Clearance:0||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:RPB8(YOR224C)|FD-Score:-3.35|P-value:4.10E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPN6(YDL097C)|FD-Score:-3.96|P-value:3.83E-5|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:RPS2(YGL123W)|FD-Score:-6.45|P-value:5.52E-11|Clearance:0||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:SPC3(YLR066W)|FD-Score:3.1|P-value:9.65E-4|Clearance:0.18||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23 Gene:SUP35(YDR172W)|FD-Score:-3.71|P-value:1.04E-4|Clearance:0||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:YCL041C(YCL041C_d)|FD-Score:4.35|P-value:6.84E-6|Clearance:0.58||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps both the verified gene PDI1/YCL043C and the uncharacterized gene YCL042W Gene:GCD1(YOR260W)|FD-Score:3.39|P-value:3.43E-4|Clearance:0.16||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:LSG1(YGL099W)|FD-Score:4.12|P-value:1.90E-5|Clearance:0.58||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MCM3(YEL032W)|FD-Score:-4.18|P-value:1.45E-5|Clearance:0||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex Gene:MPS2(YGL075C)|FD-Score:3.24|P-value:6.08E-4|Clearance:0.13||SGD DESC:Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p Gene:NFS1(YCL017C)|FD-Score:-3.64|P-value:1.36E-4|Clearance:0||SGD DESC:Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria Gene:NMD3(YHR170W)|FD-Score:3.54|P-value:2.00E-4|Clearance:0.14||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:PUP1(YOR157C)|FD-Score:-3.3|P-value:4.86E-4|Clearance:0||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:RPB8(YOR224C)|FD-Score:-3.35|P-value:4.10E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPN6(YDL097C)|FD-Score:-3.96|P-value:3.83E-5|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:RPS2(YGL123W)|FD-Score:-6.45|P-value:5.52E-11|Clearance:0||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:SPC3(YLR066W)|FD-Score:3.1|P-value:9.65E-4|Clearance:0.18||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23 Gene:SUP35(YDR172W)|FD-Score:-3.71|P-value:1.04E-4|Clearance:0||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:YCL041C(YCL041C_d)|FD-Score:4.35|P-value:6.84E-6|Clearance:0.58||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps both the verified gene PDI1/YCL043C and the uncharacterized gene YCL042W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 25236843
Download HOP data (tab-delimited text)  (excel)
Gene:AIM26(YKL037W)|FD-Score:3.37|P-value:3.78E-4||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:AKR1(YDR264C)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:ALD5(YER073W)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Mitochondrial aldehyde dehydrogenase, involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed Gene:APN1(YKL114C)|FD-Score:-4.41|P-value:5.09E-6||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ARE2(YNR019W)|FD-Score:3.95|P-value:3.94E-5||SGD DESC:Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the presence of oxygen; ARE2 has a paralog, ARE1, that arose from the whole genome duplication Gene:ARO1(YDR127W)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATG7(YHR171W)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation Gene:BNA3(YJL060W)|FD-Score:4.77|P-value:9.31E-7||SGD DESC:Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate Gene:CBP3(YPL215W)|FD-Score:3.4|P-value:3.31E-4||SGD DESC:Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex Gene:DFG16(YOR030W)|FD-Score:3.38|P-value:3.60E-4||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DPH5(YLR172C)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm Gene:EFT2(YDR385W)|FD-Score:-3.66|P-value:1.25E-4||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT2 has a paralog, EFT1, that arose from the whole genome duplication Gene:GLY1(YEL046C)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:HEL1(YKR017C_p)|FD-Score:4.45|P-value:4.20E-6||SGD DESC:RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU) Gene:HMS2(YJR147W)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Protein with similarity to heat shock transcription factors; overexpression suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant; HMS2 has a paralog, SKN7, that arose from the whole genome duplication Gene:IME4(YGL192W)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:IRC13(YOR235W_d)|FD-Score:-3.83|P-value:6.42E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:IST1(YNL265C)|FD-Score:4.42|P-value:5.02E-6||SGD DESC:Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p Gene:KAP104(YBR017C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Transportin or cytosolic karyopherin beta 2; functions in the rg-nuclear localization signal-mediated nuclear import/reimport of mRNA-binding proteins Nab2p and Hrp1p; regulates asymmetric protein synthesis in daughter cells during mitosis Gene:MDM34(YGL219C)|FD-Score:3.15|P-value:8.20E-4||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MIC14(YDR031W)|FD-Score:3.84|P-value:6.20E-5||SGD DESC:Mitochondrial intermembrane space protein of unknown function; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MRPS5(YBR251W)|FD-Score:-3.16|P-value:7.79E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NSG1(YHR133C)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins; forms foci at the nuclear periphery upon DNA replication stress Gene:NUP60(YAR002W)|FD-Score:-3.35|P-value:3.98E-4||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; both NUP1 and NUP60 are homologous to human NUP153 Gene:PEP8(YJL053W)|FD-Score:-3.55|P-value:1.94E-4||SGD DESC:Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress Gene:PHO80(YOL001W)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:PHO81(YGR233C)|FD-Score:3.23|P-value:6.29E-4||SGD DESC:Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress Gene:PHO88(YBR106W)|FD-Score:-4.04|P-value:2.68E-5||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PMT2(YAL023C)|FD-Score:-3.99|P-value:3.36E-5||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication Gene:PUS1(YPL212C)|FD-Score:-4.26|P-value:1.03E-5||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; nuclear protein that appears to be involved in tRNA export; also acts on U2 snRNA Gene:RCF2(YNR018W)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast Gene:RIC1(YLR039C)|FD-Score:4.89|P-value:5.09E-7||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPS18A(YDR450W)|FD-Score:3.82|P-value:6.66E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress Gene:RPS1A(YLR441C)|FD-Score:-3.44|P-value:2.91E-4||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication Gene:RRG1(YDR065W)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Protein of unknown function, required for vacuolar acidification and mitochondrial genome maintenance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RSM23(YGL129C)|FD-Score:-3.16|P-value:7.78E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to mammalian apoptosis mediator proteins; null mutation prevents induction of apoptosis by overproduction of metacaspase Mca1p Gene:SIA1(YOR137C)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Protein of unassigned function involved in activation of the Pma1p plasma membrane H+-ATPase by glucose Gene:SIC1(YLR079W)|FD-Score:-3.87|P-value:5.36E-5||SGD DESC:Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 Gene:SKY1(YMR216C)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SNF2(YOR290C)|FD-Score:5.22|P-value:8.80E-8||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SPT4(YGR063C)|FD-Score:3.95|P-value:3.96E-5||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SRV2(YNL138W)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cyclase and facilitates activation by RAS; C-terminus binds ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis Gene:SSD1(YDR293C)|FD-Score:5.36|P-value:4.09E-8||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SUR4(YLR372W)|FD-Score:3.73|P-value:9.60E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SYH1(YPL105C)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:3.25|P-value:5.86E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP4(YDR354W)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:7.39|P-value:7.33E-14||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBX2(YML013W)|FD-Score:6.3|P-value:1.52E-10||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:VAN1(YML115C)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant Gene:XBP1(YIL101C)|FD-Score:-3.32|P-value:4.51E-4||SGD DESC:Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:YAL065C(YAL065C_p)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Putative protein of unknown function; has homology to FLO1; possible pseudogene Gene:YBL039W-B(YBL039W-B_p)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Putative protein of unknown function Gene:YBL059W(YBL059W_p)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication Gene:YBR277C(YBR277C_d)|FD-Score:4.33|P-value:7.49E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YCR050C(YCR050C_p)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein Gene:YDL118W(YDL118W_p)|FD-Score:-3.89|P-value:4.96E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDR203W(YDR203W_d)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER076C(YER076C_p)|FD-Score:3.15|P-value:8.25E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization Gene:YER130C(YER130C_p)|FD-Score:3.94|P-value:4.05E-5||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YGL177W(YGL177W_d)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR307C-A(YLR307C-A_p)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Putative protein of unknown function Gene:YML012C-A(YML012C-A_d)|FD-Score:-4.05|P-value:2.52E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YMR279C(YMR279C)|FD-Score:3.75|P-value:8.96E-5||SGD DESC:Putative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; YMR279C has a paralog, ATR1, that arose from the whole genome duplication Gene:YNR025C(YNR025C_d)|FD-Score:3.97|P-value:3.55E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase Gene:YSP1(YHR155W)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication Gene:ZRC1(YMR243C)|FD-Score:6.96|P-value:1.68E-12||SGD DESC:Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication Gene:AIM26(YKL037W)|FD-Score:3.37|P-value:3.78E-4||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:AKR1(YDR264C)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:ALD5(YER073W)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Mitochondrial aldehyde dehydrogenase, involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed Gene:APN1(YKL114C)|FD-Score:-4.41|P-value:5.09E-6||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ARE2(YNR019W)|FD-Score:3.95|P-value:3.94E-5||SGD DESC:Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the presence of oxygen; ARE2 has a paralog, ARE1, that arose from the whole genome duplication Gene:ARO1(YDR127W)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATG7(YHR171W)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation Gene:BNA3(YJL060W)|FD-Score:4.77|P-value:9.31E-7||SGD DESC:Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate Gene:CBP3(YPL215W)|FD-Score:3.4|P-value:3.31E-4||SGD DESC:Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex Gene:DFG16(YOR030W)|FD-Score:3.38|P-value:3.60E-4||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DPH5(YLR172C)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm Gene:EFT2(YDR385W)|FD-Score:-3.66|P-value:1.25E-4||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT2 has a paralog, EFT1, that arose from the whole genome duplication Gene:GLY1(YEL046C)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:HEL1(YKR017C_p)|FD-Score:4.45|P-value:4.20E-6||SGD DESC:RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU) Gene:HMS2(YJR147W)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Protein with similarity to heat shock transcription factors; overexpression suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant; HMS2 has a paralog, SKN7, that arose from the whole genome duplication Gene:IME4(YGL192W)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:IRC13(YOR235W_d)|FD-Score:-3.83|P-value:6.42E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:IST1(YNL265C)|FD-Score:4.42|P-value:5.02E-6||SGD DESC:Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p Gene:KAP104(YBR017C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Transportin or cytosolic karyopherin beta 2; functions in the rg-nuclear localization signal-mediated nuclear import/reimport of mRNA-binding proteins Nab2p and Hrp1p; regulates asymmetric protein synthesis in daughter cells during mitosis Gene:MDM34(YGL219C)|FD-Score:3.15|P-value:8.20E-4||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MIC14(YDR031W)|FD-Score:3.84|P-value:6.20E-5||SGD DESC:Mitochondrial intermembrane space protein of unknown function; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MRPS5(YBR251W)|FD-Score:-3.16|P-value:7.79E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NSG1(YHR133C)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins; forms foci at the nuclear periphery upon DNA replication stress Gene:NUP60(YAR002W)|FD-Score:-3.35|P-value:3.98E-4||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; both NUP1 and NUP60 are homologous to human NUP153 Gene:PEP8(YJL053W)|FD-Score:-3.55|P-value:1.94E-4||SGD DESC:Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress Gene:PHO80(YOL001W)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:PHO81(YGR233C)|FD-Score:3.23|P-value:6.29E-4||SGD DESC:Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress Gene:PHO88(YBR106W)|FD-Score:-4.04|P-value:2.68E-5||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PMT2(YAL023C)|FD-Score:-3.99|P-value:3.36E-5||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication Gene:PUS1(YPL212C)|FD-Score:-4.26|P-value:1.03E-5||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; nuclear protein that appears to be involved in tRNA export; also acts on U2 snRNA Gene:RCF2(YNR018W)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast Gene:RIC1(YLR039C)|FD-Score:4.89|P-value:5.09E-7||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPS18A(YDR450W)|FD-Score:3.82|P-value:6.66E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress Gene:RPS1A(YLR441C)|FD-Score:-3.44|P-value:2.91E-4||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication Gene:RRG1(YDR065W)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Protein of unknown function, required for vacuolar acidification and mitochondrial genome maintenance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RSM23(YGL129C)|FD-Score:-3.16|P-value:7.78E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to mammalian apoptosis mediator proteins; null mutation prevents induction of apoptosis by overproduction of metacaspase Mca1p Gene:SIA1(YOR137C)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Protein of unassigned function involved in activation of the Pma1p plasma membrane H+-ATPase by glucose Gene:SIC1(YLR079W)|FD-Score:-3.87|P-value:5.36E-5||SGD DESC:Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 Gene:SKY1(YMR216C)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SNF2(YOR290C)|FD-Score:5.22|P-value:8.80E-8||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SPT4(YGR063C)|FD-Score:3.95|P-value:3.96E-5||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SRV2(YNL138W)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cyclase and facilitates activation by RAS; C-terminus binds ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis Gene:SSD1(YDR293C)|FD-Score:5.36|P-value:4.09E-8||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SUR4(YLR372W)|FD-Score:3.73|P-value:9.60E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SYH1(YPL105C)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:3.25|P-value:5.86E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP4(YDR354W)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:7.39|P-value:7.33E-14||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBX2(YML013W)|FD-Score:6.3|P-value:1.52E-10||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:VAN1(YML115C)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant Gene:XBP1(YIL101C)|FD-Score:-3.32|P-value:4.51E-4||SGD DESC:Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:YAL065C(YAL065C_p)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Putative protein of unknown function; has homology to FLO1; possible pseudogene Gene:YBL039W-B(YBL039W-B_p)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Putative protein of unknown function Gene:YBL059W(YBL059W_p)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication Gene:YBR277C(YBR277C_d)|FD-Score:4.33|P-value:7.49E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YCR050C(YCR050C_p)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein Gene:YDL118W(YDL118W_p)|FD-Score:-3.89|P-value:4.96E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDR203W(YDR203W_d)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER076C(YER076C_p)|FD-Score:3.15|P-value:8.25E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization Gene:YER130C(YER130C_p)|FD-Score:3.94|P-value:4.05E-5||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YGL177W(YGL177W_d)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR307C-A(YLR307C-A_p)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Putative protein of unknown function Gene:YML012C-A(YML012C-A_d)|FD-Score:-4.05|P-value:2.52E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YMR279C(YMR279C)|FD-Score:3.75|P-value:8.96E-5||SGD DESC:Putative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; YMR279C has a paralog, ATR1, that arose from the whole genome duplication Gene:YNR025C(YNR025C_d)|FD-Score:3.97|P-value:3.55E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase Gene:YSP1(YHR155W)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication Gene:ZRC1(YMR243C)|FD-Score:6.96|P-value:1.68E-12||SGD DESC:Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YCL041C_d4.356.84E-60.58YCL041C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps both the verified gene PDI1/YCL043C and the uncharacterized gene YCL042W
YGL099W4.121.90E-50.58LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YHR170W3.542.00E-40.14NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YOR260W3.393.43E-40.16GCD1Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YGL075C3.246.08E-40.13MPS2Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p
YLR066W3.109.65E-40.18SPC3Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23
YJL002C2.920.001760.03OST1Alpha subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins
YNL282W2.890.001910.04POP3Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YDL045C2.850.002190.01FAD1Flavin adenine dinucleotide (FAD) synthetase, performs the second step in synthesis of FAD from riboflavin
YBL084C2.840.002290.10CDC27Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YGR075C2.730.003160.15PRP38Unique component of the U4/U6.U5 tri-snRNP particle, required for conformational changes which result in the catalytic activation of the spliceosome; dispensible for spliceosome assembly
YBL020W2.580.004990.05RFT1Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein
YDL193W2.520.005790.00NUS1Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY
YER133W2.520.005850.04GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YER013W2.480.006480.01PRP22DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A7.397.33E-14TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YMR243C6.961.68E-12ZRC1Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication
YML013W6.301.52E-10UBX2Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis
YDR293C5.364.09E-8SSD1Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function
YOR290C5.228.80E-8SNF2Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p
YLR039C4.895.09E-7RIC1Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes
YJL060W4.779.31E-7BNA3Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate
YKR017C_p4.454.20E-6HEL1_pRING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU)
YNL265C4.425.02E-6IST1Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p
YBR277C_d4.337.49E-6YBR277C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W
YMR216C4.261.01E-5SKY1SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1
YDR127W4.171.54E-5ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YNR025C_d3.973.55E-5YNR025C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase
YNR019W3.953.94E-5ARE2Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the presence of oxygen; ARE2 has a paralog, ARE1, that arose from the whole genome duplication
YGR063C3.953.96E-5SPT4Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions

GO enrichment analysis for SGTC_3038
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2227.58E-67SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.119403TSC3-RPN4
0.2004.24E-54SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.102941TSC3-RPN4
0.1884.63E-48SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.134328TSC3-RPN4
0.1797.06E-44SGTC_9262268-1401 59.4 μMChemDiv (Drug-like library)29315780.118421TSC3-RPN4
0.1701.58E-39SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.0657895TSC3-RPN4
0.1693.57E-39SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.0985916TSC3-RPN4
0.1661.69E-37SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0779221TSC3-RPN4
0.1652.81E-37SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0735294TSC3-RPN4
0.1582.47E-34SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.184211TSC3-RPN4
0.1541.54E-32SGTC_2627acetyl isogambogic acid 100.0 μMMicrosource (Natural product library)68577890.0689655TSC3-RPN4
0.1533.39E-32SGTC_2740felodipine 17.1 μMMiscellaneous33330.133333
0.1498.03E-31SGTC_4283448-4153 16.5 μMChemDiv (Drug-like library)38348740.116279TSC3-RPN4
0.1452.93E-29SGTC_1726st035556 14.6 μMTimTec (Natural product derivative library)59194390.123077
0.1454.41E-29SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.121212TSC3-RPN4
0.1441.08E-28SGTC_1662st014054 52.0 μMTimTec (Natural product derivative library)28387770.0941176TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3147909702749.47 μM0.65306125236841Chembridge (Drug-like library)299.430444.66202
SGTC_3026909063349.47 μM0.63461525236842Chembridge (Drug-like library)299.430444.72902
SGTC_3153910025849.47 μM0.57692325236848Chembridge (Drug-like library)315.429843.93603
SGTC_2807794523851.95 μM0.4754117148797Chembridge (Drug-like library)366.476584.21604
SGTC_1656st01292111 μM0.36732821TimTec (Natural product derivative library)375.14928-0.315
SGTC_3143908665849.47 μM0.325236626Chembridge (Drug-like library)300.41853.45803
SGTC_1639Lupinine o-methylbenzoicacid ester hydrochloride61.8 μM0.29230817037758TimTec (Natural product derivative library)323.857544.19413ergosterol biosynthesis
SGTC_1660st01296028.1 μM0.29230824207266TimTec (Natural product derivative library)580.4471550.052815iron homeostasis
SGTC_22957971645200 μM0.2909092978226Chembridge (Fragment library)204.268242.09911
SGTC_3149909785549.47 μM0.29032325236625Chembridge (Drug-like library)365.287983.88103Golgi