9093193

[4-[(2-chlorophenyl)methyl]piperazin-1-yl]-(5-methylthiophen-2-yl)methanone

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3044
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 25236277
SMILES CC1=CC=C(S1)C(=O)N2CCN(CC2)CC3=CC=CC=C3Cl
Standardized SMILES Cc1ccc(s1)C(=O)N2CCN(Cc3ccccc3Cl)CC2
Molecular weight 334.8636
ALogP 3.55
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.98
% growth inhibition (Hom. pool) 6.93


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 25236277
Download HIP data (tab-delimited text)  (excel)
Gene:ALG14(YBR070C)|FD-Score:-3.39|P-value:3.46E-4|Clearance:0||SGD DESC:Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases Gene:CDC28(YBR160W)|FD-Score:3.78|P-value:7.90E-5|Clearance:0.07||SGD DESC:Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates; involved in modulating membrane trafficking dynamics; protein abundance increases in response to DNA replication stress Gene:ERG12(YMR208W)|FD-Score:3.23|P-value:6.28E-4|Clearance:0.04||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:LSG1(YGL099W)|FD-Score:-3.56|P-value:1.84E-4|Clearance:0||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MTR3(YGR158C)|FD-Score:3.71|P-value:1.03E-4|Clearance:0.07||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NOG2(YNR053C)|FD-Score:4.48|P-value:3.65E-6|Clearance:0.46||SGD DESC:Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation Gene:PMI40(YER003C)|FD-Score:3.59|P-value:1.65E-4|Clearance:0.03||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:PRE7(YBL041W)|FD-Score:3.11|P-value:9.51E-4|Clearance:0.12||SGD DESC:Beta 6 subunit of the 20S proteasome Gene:PRP31(YGR091W)|FD-Score:3.64|P-value:1.35E-4|Clearance:0.05||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:PRP43(YGL120C)|FD-Score:3.39|P-value:3.50E-4|Clearance:0.16||SGD DESC:RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome Gene:RPP1(YHR062C)|FD-Score:3.17|P-value:7.67E-4|Clearance:0.02||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RSC58(YLR033W)|FD-Score:-3.23|P-value:6.11E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SMC1(YFL008W)|FD-Score:-3.55|P-value:1.95E-4|Clearance:0||SGD DESC:Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure Gene:SME1(YOR159C)|FD-Score:4.7|P-value:1.28E-6|Clearance:0.46||SGD DESC:Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E Gene:SPC42(YKL042W)|FD-Score:-3.43|P-value:3.01E-4|Clearance:0||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:SPT15(YER148W)|FD-Score:4.01|P-value:3.07E-5|Clearance:0.23||SGD DESC:TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability Gene:SUA7(YPR086W)|FD-Score:3.55|P-value:1.90E-4|Clearance:0.16||SGD DESC:Transcription factor TFIIB, a general transcription factor required for transcription initiation and start site selection by RNA polymerase II Gene:TFC6(YDR362C)|FD-Score:-3.17|P-value:7.72E-4|Clearance:0||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:TIF35(YDR429C)|FD-Score:4.03|P-value:2.81E-5|Clearance:0.02||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TIM10(YHR005C-A)|FD-Score:3.14|P-value:8.58E-4|Clearance:0.03||SGD DESC:Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:TSR1(YDL060W)|FD-Score:-3.91|P-value:4.56E-5|Clearance:0||SGD DESC:Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:UGP1(YKL035W)|FD-Score:3.18|P-value:7.40E-4|Clearance:0.01||SGD DESC:UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication Gene:UTP4(YDR324C)|FD-Score:3.14|P-value:8.35E-4|Clearance:0.01||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:YKL153W(YKL153W_d)|FD-Score:3.19|P-value:7.10E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant Gene:YPR136C(YPR136C_d)|FD-Score:3.56|P-value:1.82E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9 Gene:ALG14(YBR070C)|FD-Score:-3.39|P-value:3.46E-4|Clearance:0||SGD DESC:Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases Gene:CDC28(YBR160W)|FD-Score:3.78|P-value:7.90E-5|Clearance:0.07||SGD DESC:Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates; involved in modulating membrane trafficking dynamics; protein abundance increases in response to DNA replication stress Gene:ERG12(YMR208W)|FD-Score:3.23|P-value:6.28E-4|Clearance:0.04||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:LSG1(YGL099W)|FD-Score:-3.56|P-value:1.84E-4|Clearance:0||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MTR3(YGR158C)|FD-Score:3.71|P-value:1.03E-4|Clearance:0.07||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NOG2(YNR053C)|FD-Score:4.48|P-value:3.65E-6|Clearance:0.46||SGD DESC:Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation Gene:PMI40(YER003C)|FD-Score:3.59|P-value:1.65E-4|Clearance:0.03||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:PRE7(YBL041W)|FD-Score:3.11|P-value:9.51E-4|Clearance:0.12||SGD DESC:Beta 6 subunit of the 20S proteasome Gene:PRP31(YGR091W)|FD-Score:3.64|P-value:1.35E-4|Clearance:0.05||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:PRP43(YGL120C)|FD-Score:3.39|P-value:3.50E-4|Clearance:0.16||SGD DESC:RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome Gene:RPP1(YHR062C)|FD-Score:3.17|P-value:7.67E-4|Clearance:0.02||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RSC58(YLR033W)|FD-Score:-3.23|P-value:6.11E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SMC1(YFL008W)|FD-Score:-3.55|P-value:1.95E-4|Clearance:0||SGD DESC:Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure Gene:SME1(YOR159C)|FD-Score:4.7|P-value:1.28E-6|Clearance:0.46||SGD DESC:Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E Gene:SPC42(YKL042W)|FD-Score:-3.43|P-value:3.01E-4|Clearance:0||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:SPT15(YER148W)|FD-Score:4.01|P-value:3.07E-5|Clearance:0.23||SGD DESC:TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability Gene:SUA7(YPR086W)|FD-Score:3.55|P-value:1.90E-4|Clearance:0.16||SGD DESC:Transcription factor TFIIB, a general transcription factor required for transcription initiation and start site selection by RNA polymerase II Gene:TFC6(YDR362C)|FD-Score:-3.17|P-value:7.72E-4|Clearance:0||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:TIF35(YDR429C)|FD-Score:4.03|P-value:2.81E-5|Clearance:0.02||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TIM10(YHR005C-A)|FD-Score:3.14|P-value:8.58E-4|Clearance:0.03||SGD DESC:Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:TSR1(YDL060W)|FD-Score:-3.91|P-value:4.56E-5|Clearance:0||SGD DESC:Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:UGP1(YKL035W)|FD-Score:3.18|P-value:7.40E-4|Clearance:0.01||SGD DESC:UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication Gene:UTP4(YDR324C)|FD-Score:3.14|P-value:8.35E-4|Clearance:0.01||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:YKL153W(YKL153W_d)|FD-Score:3.19|P-value:7.10E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant Gene:YPR136C(YPR136C_d)|FD-Score:3.56|P-value:1.82E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 25236277
Download HOP data (tab-delimited text)  (excel)
Gene:ADE3(YGR204W)|FD-Score:3.74|P-value:9.31E-5||SGD DESC:Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine Gene:ADK1(YDR226W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress Gene:AFT1(YGL071W)|FD-Score:4.27|P-value:9.74E-6||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARR3(YPR201W)|FD-Score:4.38|P-value:5.99E-6||SGD DESC:Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite Gene:ASC1(YMR116C)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation Gene:BIO3(YNR058W)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:7,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:BUL1(YMR275C)|FD-Score:3.93|P-value:4.23E-5||SGD DESC:Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex; disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases; BUL1 has a paralog, BUL2, that arose from the whole genome duplication Gene:CAM1(YPL048W)|FD-Score:3.21|P-value:6.57E-4||SGD DESC:Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma Gene:CCE1(YKL011C)|FD-Score:4.91|P-value:4.60E-7||SGD DESC:Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA Gene:CDC50(YCR094W)|FD-Score:-6.79|P-value:5.52E-12||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CEX1(YOR112W)|FD-Score:3.74|P-value:9.12E-5||SGD DESC:Cytoplasmic component of the nuclear aminoacylation-dependent tRNA export pathway; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a and the RAN GTPase Gsp1p Gene:CHS3(YBR023C)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan Gene:CIT2(YCR005C)|FD-Score:-5.21|P-value:9.32E-8||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate, peroxisomal isozyme involved in glyoxylate cycle; expression is controlled by Rtg1p and Rtg2p transcription factors; CIT2 has a paralog, CIT1, that arose from the whole genome duplication Gene:DEF1(YKL054C)|FD-Score:5.2|P-value:1.01E-7||SGD DESC:RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII present in an elongation complex; mutant is deficient in Zip1p loading onto chromosomes during meiosis Gene:DLD2(YDL178W)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:D-lactate dehydrogenase, located in the mitochondrial matrix Gene:END3(YNL084C)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:EXO1(YOR033C)|FD-Score:-3.23|P-value:6.24E-4||SGD DESC:5'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair and DNA mismatch repair; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress Gene:FBP1(YLR377C)|FD-Score:-3.34|P-value:4.24E-4||SGD DESC:Fructose-1,6-bisphosphatase, key regulatory enzyme in the gluconeogenesis pathway, required for glucose metabolism; undergoes either proteasome-mediated or autophagy-mediated degradation depending on growth conditions; interacts with Vid30p Gene:FET4(YMR319C)|FD-Score:4.93|P-value:4.14E-7||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FMT1(YBL013W)|FD-Score:-3.35|P-value:4.00E-4||SGD DESC:Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate Gene:FRA1(YLL029W)|FD-Score:3.9|P-value:4.75E-5||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation Gene:FUI1(YBL042C)|FD-Score:-3.28|P-value:5.22E-4||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GID8(YMR135C)|FD-Score:5.19|P-value:1.06E-7||SGD DESC:Subunit of GID Complex, binds strongly to central component Vid30p; GID Complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; recruits Rmd5p, Fyv10 and Vid28p to GID Complex; contains LisH, CTLH, and CRA domains that mediate binding to Vid30p (LisH) and Rmd5p and Vid28p (CTLH and CRA); dosage-dependent regulator of START Gene:GLK1(YCL040W)|FD-Score:4.77|P-value:8.99E-7||SGD DESC:Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication Gene:GPT2(YKR067W)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:GYP1(YOR070C)|FD-Score:5.48|P-value:2.12E-8||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HAP2(YGL237C)|FD-Score:-3.14|P-value:8.55E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding Gene:HBS1(YKR084C)|FD-Score:3.24|P-value:5.93E-4||SGD DESC:GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled; genetically implicated in mRNA no-go decay Gene:HER2(YMR293C)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase Gene:HIS4(YCL030C)|FD-Score:-3.71|P-value:1.04E-4||SGD DESC:Multifunctional enzyme containing phosphoribosyl-ATP pyrophosphatase, phosphoribosyl-AMP cyclohydrolase, and histidinol dehydrogenase activities; catalyzes the second, third, ninth and tenth steps in histidine biosynthesis Gene:HLJ1(YMR161W)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ Gene:HMO1(YDR174W)|FD-Score:5.85|P-value:2.47E-9||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:IDH2(YOR136W)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:IRC4(YDR540C)|FD-Score:-4.92|P-value:4.35E-7||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:JEM1(YJL073W)|FD-Score:-3.16|P-value:7.91E-4||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:LEO1(YOR123C)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:MGR3(YMR115W)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr1p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA Gene:MMS22(YLR320W)|FD-Score:-4.16|P-value:1.56E-5||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation Gene:MON1(YGL124C)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate Gene:MRPL38(YKL170W)|FD-Score:4.74|P-value:1.09E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:MSA1(YOR066W)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Activator of G1-specific transcription factors, MBF and SBF; involved in regulation of the timing of G1-specific gene transcription and cell cycle initiation; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:MSB1(YOR188W)|FD-Score:4.59|P-value:2.25E-6||SGD DESC:Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:PET10(YKR046C)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Protein of unknown function that co-purifies with lipid particles; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange Gene:PET100(YDR079W)|FD-Score:5.04|P-value:2.36E-7||SGD DESC:Chaperone that specifically facilitates the assembly of cytochrome c oxidase, integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme Gene:PEX6(YNL329C)|FD-Score:3.77|P-value:8.28E-5||SGD DESC:AAA-peroxin that heterodimerizes with AAA-peroxin Pex1p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol Gene:PIN2(YOR104W)|FD-Score:3.19|P-value:7.03E-4||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced; predicted to be palmitoylated Gene:PTC1(YDL006W)|FD-Score:-3.79|P-value:7.49E-5||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:PUF3(YLL013C)|FD-Score:-3.42|P-value:3.14E-4||SGD DESC:Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins Gene:RAD54(YGL163C)|FD-Score:4.65|P-value:1.68E-6||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:RCN1(YKL159C)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Protein involved in calcineurin regulation during calcium signaling; has similarity to H. sapiens DSCR1 which is found in the Down Syndrome candidate region Gene:RGC1(YPR115W)|FD-Score:4.06|P-value:2.50E-5||SGD DESC:Pleckstrin homology domain containing protein; proposed to function as a glycerol channel activator; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; RGC1 has a paralog, ASK10, that arose from the whole genome duplication Gene:RIC1(YLR039C)|FD-Score:3.82|P-value:6.58E-5||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RIT1(YMR283C)|FD-Score:-4.43|P-value:4.76E-6||SGD DESC:2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA Gene:ROM1(YGR070W)|FD-Score:-3.15|P-value:8.30E-4||SGD DESC:GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP Gene:RPA12(YJR063W)|FD-Score:4.57|P-value:2.42E-6||SGD DESC:RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex Gene:RPL13B(YMR142C)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication Gene:RPL21B(YPL079W)|FD-Score:3.19|P-value:7.05E-4||SGD DESC:Ribosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication Gene:RPS25A(YGR027C)|FD-Score:-3.18|P-value:7.36E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication Gene:RPS26B(YER131W)|FD-Score:-3.25|P-value:5.76E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S26, no bacterial homolog; RPS26B has a paralog, RPS26A, that arose from the whole genome duplication Gene:RTS1(YOR014W)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:RXT2(YBR095C)|FD-Score:-4.07|P-value:2.32E-5||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SPO22(YIL073C)|FD-Score:-3.14|P-value:8.45E-4||SGD DESC:Meiosis-specific protein essential for chromosome synapsis, involved in completion of nuclear divisions during meiosis; induced early in meiosis Gene:SPS100(YHR139C)|FD-Score:-5.15|P-value:1.33E-7||SGD DESC:Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin Gene:SRB8(YCR081W)|FD-Score:-3.16|P-value:7.85E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:SSK1(YLR006C)|FD-Score:-3.35|P-value:4.06E-4||SGD DESC:Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate Gene:SWI4(YER111C)|FD-Score:-3.82|P-value:6.61E-5||SGD DESC:DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p Gene:TAE2(YPL009C)|FD-Score:-3.3|P-value:4.81E-4||SGD DESC:Protein of unknown function, involved in protein translation; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:TAT1(YBR069C)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TPM2(YIL138C)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Minor isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; appears to have distinct and also overlapping functions with Tpm1p; TPM2 has a paralog, TPM1, that arose from the whole genome duplication Gene:TUB3(YML124C)|FD-Score:3.8|P-value:7.37E-5||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:TYW1(YPL207W)|FD-Score:3.92|P-value:4.49E-5||SGD DESC:Iron-sulfer protein required for synthesis of Wybutosine modified tRNA; Wybutosine is a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron Gene:UBX7(YBR273C)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p; UBX7 has a paralog, UBX6, that arose from the whole genome duplication Gene:URE2(YNL229C)|FD-Score:-3.15|P-value:8.18E-4||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VBA2(YBR293W)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VMS1(YDR049W)|FD-Score:3.19|P-value:7.02E-4||SGD DESC:Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans Gene:YAL004W(YAL004W_d)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C Gene:YAR030C(YAR030C_d)|FD-Score:-3.53|P-value:2.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YAR029W and the verified gene PRM9 Gene:YCP4(YCR004C)|FD-Score:-3.19|P-value:7.19E-4||SGD DESC:Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YDL177C(YDL177C_p)|FD-Score:-3.13|P-value:8.67E-4||SGD DESC:Putative protein of unknown function; similar to the mouse IMPACT gene; YDL177C is not an essential gene Gene:YEA6(YEL006W)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog Gene:YFL034W(YFL034W_p)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk Gene:YGL218W(YGL218W_d)|FD-Score:5.03|P-value:2.46E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance Gene:YGR121W-A(YGR121W-A_p)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Putative protein of unknown function Gene:YHL045W(YHL045W_d)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Putative protein of unknown function; not an essential gene Gene:YHR112C(YHR112C)|FD-Score:5.93|P-value:1.55E-9||SGD DESC:Protein of unknown function; localizes to the cytoplasm and nucleus; overexpression affects protein trafficking through the endocytic pathway Gene:YIL012W(YIL012W_d)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL165C(YIL165C_p)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:Putative protein of unknown function; mutant exhibits mitophagy defects; in closely related species and other S. cerevisiae strain backgrounds YIL165C and adjacent ORF, YIL164C, likely constitute a single ORF encoding a nitrilase gene Gene:YJL175W(YJL175W_d)|FD-Score:-3.88|P-value:5.32E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion confers resistance to cisplatin, hypersensitivity to 5-fluorouracil, and growth defect at high pH with high calcium; overlaps gene for SWI3 transcription factor Gene:YJR038C(YJR038C_d)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR033C(YKR033C_d)|FD-Score:-5.48|P-value:2.08E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene DAL80 Gene:YKR104W(YKR104W)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; contains a stop codon in S288C; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds Gene:YLR072W(YLR072W_p)|FD-Score:3.85|P-value:5.91E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YLR072W is not an esssential gene Gene:YLR446W(YLR446W_p)|FD-Score:6.73|P-value:8.26E-12||SGD DESC:Putative hexokinase; transcript is upregulated during sporulation and the unfolded protein response; YLR446W is not an essential gene Gene:YML012C-A(YML012C-A_d)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YMR001C-A(YMR001C-A_p)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:Putative protein of unknown function Gene:YMR013W-A(YMR013W-A_d)|FD-Score:4.96|P-value:3.56E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the characterized snoRNA gene snR73 Gene:YMR027W(YMR027W_p)|FD-Score:3.95|P-value:3.98E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR027W is not an essential gene Gene:YNL203C(YNL203C_d)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL150C(YOL150C_d)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR376W-A(YOR376W-A_p)|FD-Score:-3.11|P-value:9.44E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPL014W(YPL014W_p)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YPR126C(YPR126C_d)|FD-Score:-3.29|P-value:4.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR130C(YPR130C_d)|FD-Score:5.71|P-value:5.78E-9||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ADE3(YGR204W)|FD-Score:3.74|P-value:9.31E-5||SGD DESC:Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine Gene:ADK1(YDR226W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress Gene:AFT1(YGL071W)|FD-Score:4.27|P-value:9.74E-6||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARR3(YPR201W)|FD-Score:4.38|P-value:5.99E-6||SGD DESC:Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite Gene:ASC1(YMR116C)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation Gene:BIO3(YNR058W)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:7,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:BUL1(YMR275C)|FD-Score:3.93|P-value:4.23E-5||SGD DESC:Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex; disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases; BUL1 has a paralog, BUL2, that arose from the whole genome duplication Gene:CAM1(YPL048W)|FD-Score:3.21|P-value:6.57E-4||SGD DESC:Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma Gene:CCE1(YKL011C)|FD-Score:4.91|P-value:4.60E-7||SGD DESC:Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA Gene:CDC50(YCR094W)|FD-Score:-6.79|P-value:5.52E-12||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CEX1(YOR112W)|FD-Score:3.74|P-value:9.12E-5||SGD DESC:Cytoplasmic component of the nuclear aminoacylation-dependent tRNA export pathway; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a and the RAN GTPase Gsp1p Gene:CHS3(YBR023C)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan Gene:CIT2(YCR005C)|FD-Score:-5.21|P-value:9.32E-8||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate, peroxisomal isozyme involved in glyoxylate cycle; expression is controlled by Rtg1p and Rtg2p transcription factors; CIT2 has a paralog, CIT1, that arose from the whole genome duplication Gene:DEF1(YKL054C)|FD-Score:5.2|P-value:1.01E-7||SGD DESC:RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII present in an elongation complex; mutant is deficient in Zip1p loading onto chromosomes during meiosis Gene:DLD2(YDL178W)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:D-lactate dehydrogenase, located in the mitochondrial matrix Gene:END3(YNL084C)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:EXO1(YOR033C)|FD-Score:-3.23|P-value:6.24E-4||SGD DESC:5'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair and DNA mismatch repair; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress Gene:FBP1(YLR377C)|FD-Score:-3.34|P-value:4.24E-4||SGD DESC:Fructose-1,6-bisphosphatase, key regulatory enzyme in the gluconeogenesis pathway, required for glucose metabolism; undergoes either proteasome-mediated or autophagy-mediated degradation depending on growth conditions; interacts with Vid30p Gene:FET4(YMR319C)|FD-Score:4.93|P-value:4.14E-7||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FMT1(YBL013W)|FD-Score:-3.35|P-value:4.00E-4||SGD DESC:Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate Gene:FRA1(YLL029W)|FD-Score:3.9|P-value:4.75E-5||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation Gene:FUI1(YBL042C)|FD-Score:-3.28|P-value:5.22E-4||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GID8(YMR135C)|FD-Score:5.19|P-value:1.06E-7||SGD DESC:Subunit of GID Complex, binds strongly to central component Vid30p; GID Complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; recruits Rmd5p, Fyv10 and Vid28p to GID Complex; contains LisH, CTLH, and CRA domains that mediate binding to Vid30p (LisH) and Rmd5p and Vid28p (CTLH and CRA); dosage-dependent regulator of START Gene:GLK1(YCL040W)|FD-Score:4.77|P-value:8.99E-7||SGD DESC:Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication Gene:GPT2(YKR067W)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:GYP1(YOR070C)|FD-Score:5.48|P-value:2.12E-8||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HAP2(YGL237C)|FD-Score:-3.14|P-value:8.55E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding Gene:HBS1(YKR084C)|FD-Score:3.24|P-value:5.93E-4||SGD DESC:GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled; genetically implicated in mRNA no-go decay Gene:HER2(YMR293C)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase Gene:HIS4(YCL030C)|FD-Score:-3.71|P-value:1.04E-4||SGD DESC:Multifunctional enzyme containing phosphoribosyl-ATP pyrophosphatase, phosphoribosyl-AMP cyclohydrolase, and histidinol dehydrogenase activities; catalyzes the second, third, ninth and tenth steps in histidine biosynthesis Gene:HLJ1(YMR161W)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ Gene:HMO1(YDR174W)|FD-Score:5.85|P-value:2.47E-9||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:IDH2(YOR136W)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:IRC4(YDR540C)|FD-Score:-4.92|P-value:4.35E-7||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:JEM1(YJL073W)|FD-Score:-3.16|P-value:7.91E-4||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:LEO1(YOR123C)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:MGR3(YMR115W)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr1p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA Gene:MMS22(YLR320W)|FD-Score:-4.16|P-value:1.56E-5||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation Gene:MON1(YGL124C)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate Gene:MRPL38(YKL170W)|FD-Score:4.74|P-value:1.09E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:MSA1(YOR066W)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Activator of G1-specific transcription factors, MBF and SBF; involved in regulation of the timing of G1-specific gene transcription and cell cycle initiation; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:MSB1(YOR188W)|FD-Score:4.59|P-value:2.25E-6||SGD DESC:Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:PET10(YKR046C)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Protein of unknown function that co-purifies with lipid particles; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange Gene:PET100(YDR079W)|FD-Score:5.04|P-value:2.36E-7||SGD DESC:Chaperone that specifically facilitates the assembly of cytochrome c oxidase, integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme Gene:PEX6(YNL329C)|FD-Score:3.77|P-value:8.28E-5||SGD DESC:AAA-peroxin that heterodimerizes with AAA-peroxin Pex1p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol Gene:PIN2(YOR104W)|FD-Score:3.19|P-value:7.03E-4||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced; predicted to be palmitoylated Gene:PTC1(YDL006W)|FD-Score:-3.79|P-value:7.49E-5||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:PUF3(YLL013C)|FD-Score:-3.42|P-value:3.14E-4||SGD DESC:Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins Gene:RAD54(YGL163C)|FD-Score:4.65|P-value:1.68E-6||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:RCN1(YKL159C)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Protein involved in calcineurin regulation during calcium signaling; has similarity to H. sapiens DSCR1 which is found in the Down Syndrome candidate region Gene:RGC1(YPR115W)|FD-Score:4.06|P-value:2.50E-5||SGD DESC:Pleckstrin homology domain containing protein; proposed to function as a glycerol channel activator; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; RGC1 has a paralog, ASK10, that arose from the whole genome duplication Gene:RIC1(YLR039C)|FD-Score:3.82|P-value:6.58E-5||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RIT1(YMR283C)|FD-Score:-4.43|P-value:4.76E-6||SGD DESC:2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA Gene:ROM1(YGR070W)|FD-Score:-3.15|P-value:8.30E-4||SGD DESC:GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP Gene:RPA12(YJR063W)|FD-Score:4.57|P-value:2.42E-6||SGD DESC:RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex Gene:RPL13B(YMR142C)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication Gene:RPL21B(YPL079W)|FD-Score:3.19|P-value:7.05E-4||SGD DESC:Ribosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication Gene:RPS25A(YGR027C)|FD-Score:-3.18|P-value:7.36E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication Gene:RPS26B(YER131W)|FD-Score:-3.25|P-value:5.76E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S26, no bacterial homolog; RPS26B has a paralog, RPS26A, that arose from the whole genome duplication Gene:RTS1(YOR014W)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:RXT2(YBR095C)|FD-Score:-4.07|P-value:2.32E-5||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SPO22(YIL073C)|FD-Score:-3.14|P-value:8.45E-4||SGD DESC:Meiosis-specific protein essential for chromosome synapsis, involved in completion of nuclear divisions during meiosis; induced early in meiosis Gene:SPS100(YHR139C)|FD-Score:-5.15|P-value:1.33E-7||SGD DESC:Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin Gene:SRB8(YCR081W)|FD-Score:-3.16|P-value:7.85E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:SSK1(YLR006C)|FD-Score:-3.35|P-value:4.06E-4||SGD DESC:Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate Gene:SWI4(YER111C)|FD-Score:-3.82|P-value:6.61E-5||SGD DESC:DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p Gene:TAE2(YPL009C)|FD-Score:-3.3|P-value:4.81E-4||SGD DESC:Protein of unknown function, involved in protein translation; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:TAT1(YBR069C)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TPM2(YIL138C)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Minor isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; appears to have distinct and also overlapping functions with Tpm1p; TPM2 has a paralog, TPM1, that arose from the whole genome duplication Gene:TUB3(YML124C)|FD-Score:3.8|P-value:7.37E-5||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:TYW1(YPL207W)|FD-Score:3.92|P-value:4.49E-5||SGD DESC:Iron-sulfer protein required for synthesis of Wybutosine modified tRNA; Wybutosine is a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron Gene:UBX7(YBR273C)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p; UBX7 has a paralog, UBX6, that arose from the whole genome duplication Gene:URE2(YNL229C)|FD-Score:-3.15|P-value:8.18E-4||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VBA2(YBR293W)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VMS1(YDR049W)|FD-Score:3.19|P-value:7.02E-4||SGD DESC:Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans Gene:YAL004W(YAL004W_d)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C Gene:YAR030C(YAR030C_d)|FD-Score:-3.53|P-value:2.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YAR029W and the verified gene PRM9 Gene:YCP4(YCR004C)|FD-Score:-3.19|P-value:7.19E-4||SGD DESC:Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YDL177C(YDL177C_p)|FD-Score:-3.13|P-value:8.67E-4||SGD DESC:Putative protein of unknown function; similar to the mouse IMPACT gene; YDL177C is not an essential gene Gene:YEA6(YEL006W)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog Gene:YFL034W(YFL034W_p)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk Gene:YGL218W(YGL218W_d)|FD-Score:5.03|P-value:2.46E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance Gene:YGR121W-A(YGR121W-A_p)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Putative protein of unknown function Gene:YHL045W(YHL045W_d)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Putative protein of unknown function; not an essential gene Gene:YHR112C(YHR112C)|FD-Score:5.93|P-value:1.55E-9||SGD DESC:Protein of unknown function; localizes to the cytoplasm and nucleus; overexpression affects protein trafficking through the endocytic pathway Gene:YIL012W(YIL012W_d)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL165C(YIL165C_p)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:Putative protein of unknown function; mutant exhibits mitophagy defects; in closely related species and other S. cerevisiae strain backgrounds YIL165C and adjacent ORF, YIL164C, likely constitute a single ORF encoding a nitrilase gene Gene:YJL175W(YJL175W_d)|FD-Score:-3.88|P-value:5.32E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion confers resistance to cisplatin, hypersensitivity to 5-fluorouracil, and growth defect at high pH with high calcium; overlaps gene for SWI3 transcription factor Gene:YJR038C(YJR038C_d)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR033C(YKR033C_d)|FD-Score:-5.48|P-value:2.08E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene DAL80 Gene:YKR104W(YKR104W)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; contains a stop codon in S288C; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds Gene:YLR072W(YLR072W_p)|FD-Score:3.85|P-value:5.91E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YLR072W is not an esssential gene Gene:YLR446W(YLR446W_p)|FD-Score:6.73|P-value:8.26E-12||SGD DESC:Putative hexokinase; transcript is upregulated during sporulation and the unfolded protein response; YLR446W is not an essential gene Gene:YML012C-A(YML012C-A_d)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YMR001C-A(YMR001C-A_p)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:Putative protein of unknown function Gene:YMR013W-A(YMR013W-A_d)|FD-Score:4.96|P-value:3.56E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the characterized snoRNA gene snR73 Gene:YMR027W(YMR027W_p)|FD-Score:3.95|P-value:3.98E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR027W is not an essential gene Gene:YNL203C(YNL203C_d)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL150C(YOL150C_d)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR376W-A(YOR376W-A_p)|FD-Score:-3.11|P-value:9.44E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPL014W(YPL014W_p)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YPR126C(YPR126C_d)|FD-Score:-3.29|P-value:4.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR130C(YPR130C_d)|FD-Score:5.71|P-value:5.78E-9||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR159C4.701.28E-60.46SME1Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
YNR053C4.483.65E-60.46NOG2Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation
YDR429C4.032.81E-50.02TIF35eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation
YER148W4.013.07E-50.23SPT15TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability
YBR160W3.787.90E-50.07CDC28Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates; involved in modulating membrane trafficking dynamics; protein abundance increases in response to DNA replication stress
YGR158C3.711.03E-40.07MTR3Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
YGR091W3.641.35E-40.05PRP31Splicing factor, component of the U4/U6-U5 snRNP complex
YER003C3.591.65E-40.03PMI40Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation
YPR136C_d3.561.82E-40.01YPR136C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9
YPR086W3.551.90E-40.17SUA7Transcription factor TFIIB, a general transcription factor required for transcription initiation and start site selection by RNA polymerase II
YGL120C3.393.50E-40.16PRP43RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome
YMR208W3.236.28E-40.04ERG12Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YKL153W_d3.197.10E-40.01YKL153W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant
YKL035W3.187.40E-40.01UGP1UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication
YHR062C3.177.67E-40.02RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR446W_p6.738.26E-12YLR446W_pPutative hexokinase; transcript is upregulated during sporulation and the unfolded protein response; YLR446W is not an essential gene
YHR112C5.931.55E-9YHR112CProtein of unknown function; localizes to the cytoplasm and nucleus; overexpression affects protein trafficking through the endocytic pathway
YDR174W5.852.47E-9HMO1Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase
YPR130C_d5.715.78E-9YPR130C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YOR070C5.482.12E-8GYP1Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion
YKL054C5.201.01E-7DEF1RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII present in an elongation complex; mutant is deficient in Zip1p loading onto chromosomes during meiosis
YMR135C5.191.06E-7GID8Subunit of GID Complex, binds strongly to central component Vid30p; GID Complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; recruits Rmd5p, Fyv10 and Vid28p to GID Complex; contains LisH, CTLH, and CRA domains that mediate binding to Vid30p (LisH) and Rmd5p and Vid28p (CTLH and CRA); dosage-dependent regulator of START
YDR079W5.042.36E-7PET100Chaperone that specifically facilitates the assembly of cytochrome c oxidase, integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme
YGL218W_d5.032.46E-7YGL218W_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance
YMR013W-A_d4.963.56E-7YMR013W-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the characterized snoRNA gene snR73
YMR319C4.934.14E-7FET4Low-affinity Fe(II) transporter of the plasma membrane
YKL011C4.914.60E-7CCE1Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA
YCL040W4.778.99E-7GLK1Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication
YKL170W4.741.09E-6MRPL38Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress
YGL163C4.651.68E-6RAD54DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress

GO enrichment analysis for SGTC_3044
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0672.59E-7SGTC_10134112-3890 241.0 μMChemDiv (Drug-like library)7051710.142857TSC3-RPN4
0.0604.11E-6SGTC_1923st058458 19.1 μMTimTec (Natural product derivative library)35349820.101449
0.0594.84E-6SGTC_27875284540 71.4 μMChembridge (Drug-like library)7369580.149254
0.0561.64E-5SGTC_7560508-1999 28.3 μMChemDiv (Drug-like library)67538530.131579RPP1 & pyrimidine depletion
0.0561.97E-5SGTC_2727riluzole 51.8 μMMiscellaneous50700.0746269mitochondrial processes
0.0552.31E-5SGTC_15998-chlro-1-tetrahydronorharmanone 90.6 μMTimTec (Pure natural product library)41748400.153846
0.0543.03E-5SGTC_30049076122 71.4 μMChembridge (Drug-like library)447825510.136986
0.0543.44E-5SGTC_469w7 172.0 μMICCB bioactive library451155700.174603ERG2
0.0533.95E-5SGTC_254nsc-64875 3.8 μMMiscellaneous569930.0882353ergosterol depletion effects on membrane
0.0534.28E-5SGTC_14264048-4480 71.7 μMChemDiv (Drug-like library)142243430.14492860S ribosome export
0.0534.34E-5SGTC_1230083-0120 66.7 μMChemDiv (Drug-like library)160017010.108434
0.0526.82E-5SGTC_31809109287 49.5 μMChembridge (Drug-like library)188912710.130435
0.0501.38E-4SGTC_32509135583 49.5 μMChembridge (Drug-like library)76993530.149254
0.0491.47E-4SGTC_5611550-0330 72.9 μMChemDiv (Drug-like library)68129340.0769231
0.0491.55E-4SGTC_13973532-0190 20.1 μMChemDiv (Drug-like library)53222800.106061

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_3055909463649.47 μM0.64705925236176Chembridge (Drug-like library)334.863563.25603
SGTC_3137909735249.47 μM0.64705944120435Chembridge (Drug-like library)371.32453.9313
SGTC_3031908876249.47 μM0.630435205253Chembridge (Drug-like library)349.254324.11302
SGTC_3139909502049.47 μM0.60784325236292Chembridge (Drug-like library)318.797962.98903
SGTC_3143908665849.47 μM0.5425236626Chembridge (Drug-like library)300.41853.45803
SGTC_3041909192949.47 μM0.47272725236887Chembridge (Drug-like library)328.471664.03603redox potentiating
SGTC_14354092-078956.7 μM0.4259262248285ChemDiv (Drug-like library)344.87834.44103plasma membrane duress
SGTC_3045909295049.47 μM0.41379344120432Chembridge (Drug-like library)365.296784.31712
SGTC_2807794523851.95 μM0.41269817148797Chembridge (Drug-like library)366.476584.21604
SGTC_2300797669771.57 μM0.4117652236109Chembridge (Fragment library)238.756362.28912ERG2