9092950

[4-[(3-chlorophenyl)methyl]piperazin-1-yl]-(2-methylphenyl)methanone hydrochloride

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3045
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 44120432
SMILES CC1=CC=CC=C1C(=O)N2CCN(CC2)CC3=CC(=CC=C3)Cl.Cl
Standardized SMILES Cc1ccccc1C(=O)N2CCN(Cc3cccc(Cl)c3)CC2
Molecular weight 365.2968
ALogP 4.32
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.84
% growth inhibition (Hom. pool) 3.45


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 44120432
Download HIP data (tab-delimited text)  (excel)
Gene:CDC24(YAL041W)|FD-Score:3.35|P-value:4.05E-4|Clearance:0.09||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:CDS1(YBR029C)|FD-Score:-3.11|P-value:9.37E-4|Clearance:0||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:DIM1(YPL266W)|FD-Score:-3.15|P-value:8.20E-4|Clearance:0||SGD DESC:Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing Gene:FRQ1(YDR373W)|FD-Score:8.2|P-value:1.16E-16|Clearance:3.95||SGD DESC:N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily Gene:HSH49(YOR319W)|FD-Score:3.99|P-value:3.28E-5|Clearance:0.07||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:ILV5(YLR355C)|FD-Score:-4.12|P-value:1.89E-5|Clearance:0||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:MGE1(YOR232W)|FD-Score:-3.42|P-value:3.08E-4|Clearance:0||SGD DESC:Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE Gene:OLE1(YGL055W)|FD-Score:3.38|P-value:3.57E-4|Clearance:0.03||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PWP2(YCR057C)|FD-Score:-3.3|P-value:4.89E-4|Clearance:0||SGD DESC:Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis Gene:RPC10(YHR143W-A)|FD-Score:3.19|P-value:7.21E-4|Clearance:0.25||SGD DESC:RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress Gene:SAS10(YDL153C)|FD-Score:3.26|P-value:5.67E-4|Clearance:0.07||SGD DESC:Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance Gene:SMC2(YFR031C)|FD-Score:3.92|P-value:4.47E-5|Clearance:0.53||SGD DESC:Subunit of the condensin complex; essential SMC chromosomal ATPase family member that forms a complex with Smc4p to form the active ATPase; Smc2p/Smc4p complex binds DNA; required for clustering of tRNA genes at the nucleolus Gene:TIF11(YMR260C)|FD-Score:4.26|P-value:1.04E-5|Clearance:0.26||SGD DESC:Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3 Gene:TRR1(YDR353W)|FD-Score:-3.42|P-value:3.13E-4|Clearance:0||SGD DESC:Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication Gene:CDC24(YAL041W)|FD-Score:3.35|P-value:4.05E-4|Clearance:0.09||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:CDS1(YBR029C)|FD-Score:-3.11|P-value:9.37E-4|Clearance:0||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:DIM1(YPL266W)|FD-Score:-3.15|P-value:8.20E-4|Clearance:0||SGD DESC:Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing Gene:FRQ1(YDR373W)|FD-Score:8.2|P-value:1.16E-16|Clearance:3.95||SGD DESC:N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily Gene:HSH49(YOR319W)|FD-Score:3.99|P-value:3.28E-5|Clearance:0.07||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:ILV5(YLR355C)|FD-Score:-4.12|P-value:1.89E-5|Clearance:0||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:MGE1(YOR232W)|FD-Score:-3.42|P-value:3.08E-4|Clearance:0||SGD DESC:Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE Gene:OLE1(YGL055W)|FD-Score:3.38|P-value:3.57E-4|Clearance:0.03||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PWP2(YCR057C)|FD-Score:-3.3|P-value:4.89E-4|Clearance:0||SGD DESC:Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis Gene:RPC10(YHR143W-A)|FD-Score:3.19|P-value:7.21E-4|Clearance:0.25||SGD DESC:RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress Gene:SAS10(YDL153C)|FD-Score:3.26|P-value:5.67E-4|Clearance:0.07||SGD DESC:Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance Gene:SMC2(YFR031C)|FD-Score:3.92|P-value:4.47E-5|Clearance:0.53||SGD DESC:Subunit of the condensin complex; essential SMC chromosomal ATPase family member that forms a complex with Smc4p to form the active ATPase; Smc2p/Smc4p complex binds DNA; required for clustering of tRNA genes at the nucleolus Gene:TIF11(YMR260C)|FD-Score:4.26|P-value:1.04E-5|Clearance:0.26||SGD DESC:Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3 Gene:TRR1(YDR353W)|FD-Score:-3.42|P-value:3.13E-4|Clearance:0||SGD DESC:Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 44120432
Download HOP data (tab-delimited text)  (excel)
Gene:AHT1(YHR093W_d)|FD-Score:3.14|P-value:8.36E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; multicopy suppressor of glucose transport defects, likely due to the presence of an HXT4 regulatory element in the region Gene:AIM37(YNL100W)|FD-Score:-3.24|P-value:5.93E-4||SGD DESC:Mitochondrial inner membrane protein; component of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:APN1(YKL114C)|FD-Score:-3.19|P-value:7.03E-4||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:AST1(YBL069W)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication Gene:BDF1(YLR399C)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:CAT8(YMR280C)|FD-Score:-3.86|P-value:5.64E-5||SGD DESC:Zinc cluster transcriptional activator; necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements; relative distribution to the nucleus increases upon DNA replication stress Gene:CHZ1(YER030W)|FD-Score:3.14|P-value:8.48E-4||SGD DESC:Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif; protein abundance increases in response to DNA replication stress Gene:COA2(YPL189C-A)|FD-Score:3.81|P-value:6.88E-5||SGD DESC:Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p Gene:COX12(YLR038C)|FD-Score:-3.74|P-value:9.29E-5||SGD DESC:Subunit VIb of cytochrome c oxidase; cytochrome c oxidase is also known as respiratory Complex IV and is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of cytochrome c oxidase but not required for activity after assembly; phosphorylated; easily released from the intermembrane space, suggesting a loose association with Complex IV Gene:DAL80(YKR034W)|FD-Score:-3.27|P-value:5.38E-4||SGD DESC:Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Gzf3p; DAL80 has a paralog, GZF3, that arose from the whole genome duplication Gene:DCC1(YCL016C)|FD-Score:3.11|P-value:9.47E-4||SGD DESC:Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance Gene:DCV1(YFR012W)|FD-Score:3.84|P-value:6.06E-5||SGD DESC:Protein of unknown function; deletion mutant shows strong genetic interaction with cdc28-as1 mutant in the presence of 1-NM-PP1; DCV1 has a paralog, YOL019W, that arose from the whole genome duplication Gene:DGR2(YKL121W_p)|FD-Score:-3.24|P-value:6.07E-4||SGD DESC:Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds Gene:DIA1(YMR316W)|FD-Score:3.18|P-value:7.31E-4||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DSE1(YER124C)|FD-Score:-3.67|P-value:1.22E-4||SGD DESC:Daughter cell-specific protein, may regulate cross-talk between the mating and filamentation pathways; deletion affects cell separation after division and sensitivity to alpha-factor and drugs affecting the cell wall; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:DSE2(YHR143W)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:EMP70(YLR083C)|FD-Score:-3.18|P-value:7.28E-4||SGD DESC:Protein with a role in cellular adhesion, filamentous growth, and endosome-to-vacuole sorting; similar to Tmn2p and Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments Gene:FMO1(YHR176W)|FD-Score:-3.53|P-value:2.05E-4||SGD DESC:Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins Gene:GFD2(YCL036W)|FD-Score:6.2|P-value:2.74E-10||SGD DESC:Protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; GFD2 has a paralog, YDR514C, that arose from the whole genome duplication Gene:GTT3(YEL017W)|FD-Score:-3.59|P-value:1.64E-4||SGD DESC:Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery Gene:KAR5(YMR065W)|FD-Score:6.57|P-value:2.57E-11||SGD DESC:Protein required for nuclear membrane fusion during karyogamy, localizes to the membrane with a soluble portion in the endoplasmic reticulum lumen, may form a complex with Jem1p and Kar2p; expression of the gene is regulated by pheromone Gene:KTR1(YOR099W)|FD-Score:4.99|P-value:2.96E-7||SGD DESC:Alpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; type II membrane protein; member of the KRE2/MNT1 mannosyltransferase family; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:MDH2(YOL126C)|FD-Score:5.59|P-value:1.14E-8||SGD DESC:Cytoplasmic malate dehydrogenase, one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1 Gene:MDM20(YOL076W)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MOS1(YCL057C-A)|FD-Score:-3.21|P-value:6.58E-4||SGD DESC:Mitochondrial protein essential for proper inner membrane organization; conserved component of the mitochondrial inner membrane organizing system (MICOS, MINOS, or MitOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture; ortholog of human MINOS1 Gene:MRPL10(YNL284C)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels Gene:MRPL51(YPR100W)|FD-Score:3.21|P-value:6.53E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSN1(YOL116W)|FD-Score:-3.83|P-value:6.39E-5||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:MSS116(YDR194C)|FD-Score:4.01|P-value:3.06E-5||SGD DESC:DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing Gene:NVJ1(YHR195W)|FD-Score:3.2|P-value:6.88E-4||SGD DESC:Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) Gene:PAP2(YOL115W)|FD-Score:3.81|P-value:6.82E-5||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Trf5p Gene:PCL1(YNL289W)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; is ubiquitinated by Dma1p; phosphorylation by Pho85p targets it for degradation; localizes to sites of polarized cell growth Gene:PHO90(YJL198W)|FD-Score:3.13|P-value:8.82E-4||SGD DESC:Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication Gene:POR1(YNL055C)|FD-Score:3.21|P-value:6.55E-4||SGD DESC:Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication Gene:PRM1(YNL279W)|FD-Score:3.22|P-value:6.41E-4||SGD DESC:Pheromone-regulated multispanning membrane protein involved in membrane fusion during mating; predicted to have 5 transmembrane segments and a coiled coil domain; localizes to the shmoo tip; regulated by Ste12p Gene:PUB1(YNL016W)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; protein abundance increases in response to DNA replication stress Gene:QRI5(YLR204W)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RGP1(YDR137W)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RPL14A(YKL006W)|FD-Score:5.76|P-value:4.18E-9||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RRT2(YBR246W)|FD-Score:-3.92|P-value:4.38E-5||SGD DESC:Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription Gene:RSM22(YKL155C)|FD-Score:5.02|P-value:2.62E-7||SGD DESC:Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase Gene:SAC1(YKL212W)|FD-Score:4.25|P-value:1.09E-5||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SIN3(YOL004W)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:Component of the Sin3p-Rpd3p histone deacetylase complex, involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity Gene:SIP18(YMR175W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Phospholipid-binding hydrophilin; essential to overcome desiccation-rehydration process; expression is induced by osmotic stress Gene:SKT5(YBL061C)|FD-Score:-3.54|P-value:1.98E-4||SGD DESC:Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication Gene:STV1(YMR054W)|FD-Score:3.15|P-value:8.10E-4||SGD DESC:Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole Gene:SYO1(YDL063C)|FD-Score:-3.22|P-value:6.31E-4||SGD DESC:Transport adaptor or symportin; facilitates synchronized nuclear coimport of the two 5S-rRNA binding proteins Rpl5p and Rpl11p; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:UFO1(YML088W)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:F-box receptor protein, subunit of the Skp1-Cdc53-F-box receptor (SCF) E3 ubiquitin ligase complex; binds to phosphorylated Ho endonuclease, allowing its ubiquitylation by SCF and subsequent degradation Gene:VFA1(YER128W)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Protein that interacts with Vps4p and has a role in vacuolar sorting; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology Gene:VPS21(YOR089C)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS63(YLR261C_d)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:XBP1(YIL101C)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:YBL062W(YBL062W_d)|FD-Score:5.16|P-value:1.26E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR013C(YBR013C_p)|FD-Score:-3.29|P-value:5.08E-4||SGD DESC:Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YBR056W-A(YBR056W-A_p)|FD-Score:4.44|P-value:4.52E-6||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the dubious ORF YBR056C-B Gene:YBT1(YLL048C)|FD-Score:3.9|P-value:4.73E-5||SGD DESC:Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters Gene:YCR064C(YCR064C_d)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YDR149C(YDR149C_d)|FD-Score:-4.12|P-value:1.88E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth Gene:YDR161W(YDR161W_p)|FD-Score:5.09|P-value:1.77E-7||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YER134C(YER134C)|FD-Score:3.78|P-value:7.95E-5||SGD DESC:Magnesium-dependent acid phosphatase, member of the haloacid dehalogenase superfamily; non-essential gene Gene:YFL034W(YFL034W_p)|FD-Score:5.05|P-value:2.26E-7||SGD DESC:Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk Gene:YIR043C(YIR043C)|FD-Score:4.11|P-value:1.94E-5||SGD DESC:Possible pseudogene in strain S288C; YIR043C and the adjacent ORF, YIR044C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family Gene:YKL169C(YKL169C_d)|FD-Score:3.15|P-value:8.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YKR104W(YKR104W)|FD-Score:-3.64|P-value:1.37E-4||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; contains a stop codon in S288C; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds Gene:YLL059C(YLL059C_d)|FD-Score:3.84|P-value:6.22E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR236C(YLR236C_d)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR312C(YLR312C_p)|FD-Score:-3.42|P-value:3.17E-4||SGD DESC:Putative protein of unknown function Gene:YOL013W-A(YOL013W-A_p)|FD-Score:-4.18|P-value:1.45E-5||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YOR385W(YOR385W_p)|FD-Score:4.85|P-value:6.25E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:YPL205C(YPL205C_d)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Hypothetical protein; deletion of locus affects telomere length Gene:YPL247C(YPL247C_p)|FD-Score:-3.14|P-value:8.47E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest Gene:YPR039W(YPR039W_d)|FD-Score:3.78|P-value:7.91E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W Gene:AHT1(YHR093W_d)|FD-Score:3.14|P-value:8.36E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; multicopy suppressor of glucose transport defects, likely due to the presence of an HXT4 regulatory element in the region Gene:AIM37(YNL100W)|FD-Score:-3.24|P-value:5.93E-4||SGD DESC:Mitochondrial inner membrane protein; component of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:APN1(YKL114C)|FD-Score:-3.19|P-value:7.03E-4||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:AST1(YBL069W)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication Gene:BDF1(YLR399C)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:CAT8(YMR280C)|FD-Score:-3.86|P-value:5.64E-5||SGD DESC:Zinc cluster transcriptional activator; necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements; relative distribution to the nucleus increases upon DNA replication stress Gene:CHZ1(YER030W)|FD-Score:3.14|P-value:8.48E-4||SGD DESC:Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif; protein abundance increases in response to DNA replication stress Gene:COA2(YPL189C-A)|FD-Score:3.81|P-value:6.88E-5||SGD DESC:Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p Gene:COX12(YLR038C)|FD-Score:-3.74|P-value:9.29E-5||SGD DESC:Subunit VIb of cytochrome c oxidase; cytochrome c oxidase is also known as respiratory Complex IV and is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of cytochrome c oxidase but not required for activity after assembly; phosphorylated; easily released from the intermembrane space, suggesting a loose association with Complex IV Gene:DAL80(YKR034W)|FD-Score:-3.27|P-value:5.38E-4||SGD DESC:Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Gzf3p; DAL80 has a paralog, GZF3, that arose from the whole genome duplication Gene:DCC1(YCL016C)|FD-Score:3.11|P-value:9.47E-4||SGD DESC:Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance Gene:DCV1(YFR012W)|FD-Score:3.84|P-value:6.06E-5||SGD DESC:Protein of unknown function; deletion mutant shows strong genetic interaction with cdc28-as1 mutant in the presence of 1-NM-PP1; DCV1 has a paralog, YOL019W, that arose from the whole genome duplication Gene:DGR2(YKL121W_p)|FD-Score:-3.24|P-value:6.07E-4||SGD DESC:Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds Gene:DIA1(YMR316W)|FD-Score:3.18|P-value:7.31E-4||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DSE1(YER124C)|FD-Score:-3.67|P-value:1.22E-4||SGD DESC:Daughter cell-specific protein, may regulate cross-talk between the mating and filamentation pathways; deletion affects cell separation after division and sensitivity to alpha-factor and drugs affecting the cell wall; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:DSE2(YHR143W)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:EMP70(YLR083C)|FD-Score:-3.18|P-value:7.28E-4||SGD DESC:Protein with a role in cellular adhesion, filamentous growth, and endosome-to-vacuole sorting; similar to Tmn2p and Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments Gene:FMO1(YHR176W)|FD-Score:-3.53|P-value:2.05E-4||SGD DESC:Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins Gene:GFD2(YCL036W)|FD-Score:6.2|P-value:2.74E-10||SGD DESC:Protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; GFD2 has a paralog, YDR514C, that arose from the whole genome duplication Gene:GTT3(YEL017W)|FD-Score:-3.59|P-value:1.64E-4||SGD DESC:Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery Gene:KAR5(YMR065W)|FD-Score:6.57|P-value:2.57E-11||SGD DESC:Protein required for nuclear membrane fusion during karyogamy, localizes to the membrane with a soluble portion in the endoplasmic reticulum lumen, may form a complex with Jem1p and Kar2p; expression of the gene is regulated by pheromone Gene:KTR1(YOR099W)|FD-Score:4.99|P-value:2.96E-7||SGD DESC:Alpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; type II membrane protein; member of the KRE2/MNT1 mannosyltransferase family; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:MDH2(YOL126C)|FD-Score:5.59|P-value:1.14E-8||SGD DESC:Cytoplasmic malate dehydrogenase, one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1 Gene:MDM20(YOL076W)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MOS1(YCL057C-A)|FD-Score:-3.21|P-value:6.58E-4||SGD DESC:Mitochondrial protein essential for proper inner membrane organization; conserved component of the mitochondrial inner membrane organizing system (MICOS, MINOS, or MitOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture; ortholog of human MINOS1 Gene:MRPL10(YNL284C)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels Gene:MRPL51(YPR100W)|FD-Score:3.21|P-value:6.53E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSN1(YOL116W)|FD-Score:-3.83|P-value:6.39E-5||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:MSS116(YDR194C)|FD-Score:4.01|P-value:3.06E-5||SGD DESC:DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing Gene:NVJ1(YHR195W)|FD-Score:3.2|P-value:6.88E-4||SGD DESC:Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) Gene:PAP2(YOL115W)|FD-Score:3.81|P-value:6.82E-5||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Trf5p Gene:PCL1(YNL289W)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; is ubiquitinated by Dma1p; phosphorylation by Pho85p targets it for degradation; localizes to sites of polarized cell growth Gene:PHO90(YJL198W)|FD-Score:3.13|P-value:8.82E-4||SGD DESC:Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication Gene:POR1(YNL055C)|FD-Score:3.21|P-value:6.55E-4||SGD DESC:Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication Gene:PRM1(YNL279W)|FD-Score:3.22|P-value:6.41E-4||SGD DESC:Pheromone-regulated multispanning membrane protein involved in membrane fusion during mating; predicted to have 5 transmembrane segments and a coiled coil domain; localizes to the shmoo tip; regulated by Ste12p Gene:PUB1(YNL016W)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; protein abundance increases in response to DNA replication stress Gene:QRI5(YLR204W)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RGP1(YDR137W)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RPL14A(YKL006W)|FD-Score:5.76|P-value:4.18E-9||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RRT2(YBR246W)|FD-Score:-3.92|P-value:4.38E-5||SGD DESC:Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription Gene:RSM22(YKL155C)|FD-Score:5.02|P-value:2.62E-7||SGD DESC:Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase Gene:SAC1(YKL212W)|FD-Score:4.25|P-value:1.09E-5||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SIN3(YOL004W)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:Component of the Sin3p-Rpd3p histone deacetylase complex, involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity Gene:SIP18(YMR175W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Phospholipid-binding hydrophilin; essential to overcome desiccation-rehydration process; expression is induced by osmotic stress Gene:SKT5(YBL061C)|FD-Score:-3.54|P-value:1.98E-4||SGD DESC:Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication Gene:STV1(YMR054W)|FD-Score:3.15|P-value:8.10E-4||SGD DESC:Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole Gene:SYO1(YDL063C)|FD-Score:-3.22|P-value:6.31E-4||SGD DESC:Transport adaptor or symportin; facilitates synchronized nuclear coimport of the two 5S-rRNA binding proteins Rpl5p and Rpl11p; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:UFO1(YML088W)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:F-box receptor protein, subunit of the Skp1-Cdc53-F-box receptor (SCF) E3 ubiquitin ligase complex; binds to phosphorylated Ho endonuclease, allowing its ubiquitylation by SCF and subsequent degradation Gene:VFA1(YER128W)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Protein that interacts with Vps4p and has a role in vacuolar sorting; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology Gene:VPS21(YOR089C)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS63(YLR261C_d)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:XBP1(YIL101C)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:YBL062W(YBL062W_d)|FD-Score:5.16|P-value:1.26E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR013C(YBR013C_p)|FD-Score:-3.29|P-value:5.08E-4||SGD DESC:Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YBR056W-A(YBR056W-A_p)|FD-Score:4.44|P-value:4.52E-6||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the dubious ORF YBR056C-B Gene:YBT1(YLL048C)|FD-Score:3.9|P-value:4.73E-5||SGD DESC:Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters Gene:YCR064C(YCR064C_d)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YDR149C(YDR149C_d)|FD-Score:-4.12|P-value:1.88E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth Gene:YDR161W(YDR161W_p)|FD-Score:5.09|P-value:1.77E-7||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YER134C(YER134C)|FD-Score:3.78|P-value:7.95E-5||SGD DESC:Magnesium-dependent acid phosphatase, member of the haloacid dehalogenase superfamily; non-essential gene Gene:YFL034W(YFL034W_p)|FD-Score:5.05|P-value:2.26E-7||SGD DESC:Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk Gene:YIR043C(YIR043C)|FD-Score:4.11|P-value:1.94E-5||SGD DESC:Possible pseudogene in strain S288C; YIR043C and the adjacent ORF, YIR044C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family Gene:YKL169C(YKL169C_d)|FD-Score:3.15|P-value:8.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YKR104W(YKR104W)|FD-Score:-3.64|P-value:1.37E-4||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; contains a stop codon in S288C; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds Gene:YLL059C(YLL059C_d)|FD-Score:3.84|P-value:6.22E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR236C(YLR236C_d)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR312C(YLR312C_p)|FD-Score:-3.42|P-value:3.17E-4||SGD DESC:Putative protein of unknown function Gene:YOL013W-A(YOL013W-A_p)|FD-Score:-4.18|P-value:1.45E-5||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YOR385W(YOR385W_p)|FD-Score:4.85|P-value:6.25E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:YPL205C(YPL205C_d)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Hypothetical protein; deletion of locus affects telomere length Gene:YPL247C(YPL247C_p)|FD-Score:-3.14|P-value:8.47E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest Gene:YPR039W(YPR039W_d)|FD-Score:3.78|P-value:7.91E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR373W8.201.16E-163.95FRQ1N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily
YMR260C4.261.04E-50.26TIF11Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3
YOR319W3.993.28E-50.07HSH49U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
YFR031C3.924.47E-50.53SMC2Subunit of the condensin complex; essential SMC chromosomal ATPase family member that forms a complex with Smc4p to form the active ATPase; Smc2p/Smc4p complex binds DNA; required for clustering of tRNA genes at the nucleolus
YGL055W3.383.57E-40.03OLE1Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
YAL041W3.354.05E-40.09CDC24Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress
YDL153C3.265.67E-40.07SAS10Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance
YHR143W-A3.197.21E-40.25RPC10RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress
YPR181C2.930.001690.08SEC23GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p
YDR324C2.850.002160.14UTP4Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript
YNL247W2.720.003290.01YNL247WCysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments
YDR141C2.710.003410.05DOP1Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies
YBR247C2.650.003990.06ENP1Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus
YHR036W2.590.004740.09BRL1Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export
YGR013W2.510.006070.02SNU71Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR065W6.572.57E-11KAR5Protein required for nuclear membrane fusion during karyogamy, localizes to the membrane with a soluble portion in the endoplasmic reticulum lumen, may form a complex with Jem1p and Kar2p; expression of the gene is regulated by pheromone
YCL036W6.202.74E-10GFD2Protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; GFD2 has a paralog, YDR514C, that arose from the whole genome duplication
YKL006W5.764.18E-9RPL14ARibosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication
YOL126C5.591.14E-8MDH2Cytoplasmic malate dehydrogenase, one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1
YBL062W_d5.161.26E-7YBL062W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YDR161W_p5.091.77E-7YDR161W_pPutative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD)
YFL034W_p5.052.26E-7YFL034W_pPutative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk
YKL155C5.022.62E-7RSM22Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase
YOR099W4.992.96E-7KTR1Alpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; type II membrane protein; member of the KRE2/MNT1 mannosyltransferase family; relocalizes from vacuole to cytoplasm upon DNA replication stress
YOR385W_p4.856.25E-7YOR385W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene
YBR056W-A_p4.444.52E-6YBR056W-A_pProtein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the dubious ORF YBR056C-B
YKL212W4.251.09E-5SAC1Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism
YNL289W4.161.61E-5PCL1Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; is ubiquitinated by Dma1p; phosphorylation by Pho85p targets it for degradation; localizes to sites of polarized cell growth
YIR043C4.111.94E-5YIR043CPossible pseudogene in strain S288C; YIR043C and the adjacent ORF, YIR044C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family
YDR194C4.013.06E-5MSS116DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing

GO enrichment analysis for SGTC_3045
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0732.20E-8SGTC_5991935-0052 86.5 μMChemDiv (Drug-like library)95573110.135802
0.0612.91E-6SGTC_11513909-8316 15.3 μMChemDiv (Drug-like library)8004310.191176
0.0595.40E-6SGTC_29949053520 71.4 μMChembridge (Drug-like library)46473840.234375RPP1 & pyrimidine depletion
0.0588.41E-6SGTC_2074185-0010 4.5 μMChemDiv (Drug-like library)37617530.0526316endomembrane recycling
0.0561.98E-5SGTC_1097itavastatin 7.2 μMNIH Clinical Collection52824510.107527azole & statin
0.0543.09E-5SGTC_33099129340 25.8 μMChembridge (Drug-like library)171589870.162162
0.0526.23E-5SGTC_254nsc-64875 3.8 μMMiscellaneous569930.0882353ergosterol depletion effects on membrane
0.0527.47E-5SGTC_2513baicalein 25.6 μMMicrosource (Natural product library)52816050.104478calcium & mitochondrial duress
0.0501.15E-4SGTC_3061glucosamine 200.0 μMMiscellaneous914310.0333333
0.0501.12E-4SGTC_1941486-1323 56.9 μMChemDiv (Drug-like library)51787540.175676
0.0501.19E-4SGTC_1505k297-0037 20.6 μMChemDiv (Drug-like library)40893960.147059
0.0491.56E-4SGTC_1767st044992 70.3 μMTimTec (Natural product derivative library)96006930.0759494
0.0491.63E-4SGTC_15024534-4282 27.7 μMChemDiv (Drug-like library)32789490.169014
0.0491.64E-4SGTC_1992st073643 23.3 μMTimTec (Natural product derivative library)31612920.135135mitochondrial processes
0.0491.94E-4SGTC_218alverine citrate 93.8 μMMiscellaneous217180.163636fatty acid desaturase (OLE1)

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3049909222349.47 μM0.6734698623917Chembridge (Drug-like library)328.835843.93502
SGTC_3137909735249.47 μM0.64705944120435Chembridge (Drug-like library)371.32453.9313
SGTC_5154092-1064102 μM0.533333717353ChemDiv (Drug-like library)335.27084.77302plasma membrane duress
SGTC_1784092-080687.51 μM0.5319152169044ChemDiv (Drug-like library)328.87895.05102ergosterol depletion effects on membrane
SGTC_3031908876249.47 μM0.5205253Chembridge (Drug-like library)349.254324.11302
SGTC_11574092-039427.6 μM0.4827593659885ChemDiv (Drug-like library)358.861823.7703
SGTC_2838900268771.43 μM0.4736846457060Chembridge (Drug-like library)357.877063.64713fatty acid desaturase (OLE1)
SGTC_13752910-0797186 μM0.472727742508ChemDiv (Drug-like library)340.416162.75204
SGTC_1818st05213656.3 μM0.454545674169TimTec (Natural product derivative library)339.861784.40212NEO1-PIK1
SGTC_3054909439749.47 μM0.452832598354Chembridge (Drug-like library)330.422883.69302fatty acid desaturase (OLE1)