1682-2595

4-methyl-N-(2-nitrophenyl)sulfanylaniline

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_305
Screen concentration 20.8 μM
Source ChemDiv (Drug-like library)
PubChem CID 226033
SMILES CC1=CC=C(C=C1)NSC2=CC=CC=C2[N+](=O)[O-]
Standardized SMILES Cc1ccc(NSc2ccccc2[N+](=O)[O-])cc1
Molecular weight 260.3116
ALogP 4.35
H-bond donor count 1
H-bond acceptor count 4
Response signature RNA processing & uracil transport

Pool Growth Kinetics
% growth inhibition (Het. pool) 23.76
% growth inhibition (Hom. pool) 15.26


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 226033
Download HIP data (tab-delimited text)  (excel)
Gene:ARP4(YJL081C)|FD-Score:3.14|P-value:8.32E-4|Clearance:0.07||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:CDC19(YAL038W)|FD-Score:5.31|P-value:5.52E-8|Clearance:0||SGD DESC:Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication Gene:CDC23(YHR166C)|FD-Score:3.69|P-value:1.13E-4|Clearance:0.11||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:GLC7(YER133W)|FD-Score:4.3|P-value:8.63E-6|Clearance:0.33||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:LSM2(YBL026W)|FD-Score:3.37|P-value:3.70E-4|Clearance:0.1||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:PCF11(YDR228C)|FD-Score:3.82|P-value:6.63E-5|Clearance:0.05||SGD DESC:mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping Gene:RFA1(YAR007C)|FD-Score:3.77|P-value:8.26E-5|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination Gene:RNA14(YMR061W)|FD-Score:3.27|P-value:5.30E-4|Clearance:0.13||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RRP15(YPR143W)|FD-Score:7.13|P-value:5.10E-13|Clearance:1.82||SGD DESC:Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs Gene:RRP45(YDR280W)|FD-Score:3.77|P-value:8.31E-5|Clearance:0.08||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:SPT6(YGR116W)|FD-Score:-3.23|P-value:6.12E-4|Clearance:0||SGD DESC:Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region Gene:TAF12(YDR145W)|FD-Score:4.75|P-value:1.03E-6|Clearance:0.45||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TOR2(YKL203C)|FD-Score:3.96|P-value:3.67E-5|Clearance:0.04||SGD DESC:PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication Gene:UGP1(YKL035W)|FD-Score:3.58|P-value:1.73E-4|Clearance:0.2||SGD DESC:UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication Gene:VRG4(YGL225W)|FD-Score:3.93|P-value:4.28E-5|Clearance:0.11||SGD DESC:Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi Gene:YNL114C(YNL114C_d)|FD-Score:-3.7|P-value:1.07E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Gene:YNL150W(YNL150W_d)|FD-Score:-3.25|P-value:5.85E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking Gene:YPR142C(YPR142C_d)|FD-Score:5.31|P-value:5.55E-8|Clearance:0.56||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing Gene:ARP4(YJL081C)|FD-Score:3.14|P-value:8.32E-4|Clearance:0.07||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:CDC19(YAL038W)|FD-Score:5.31|P-value:5.52E-8|Clearance:0||SGD DESC:Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication Gene:CDC23(YHR166C)|FD-Score:3.69|P-value:1.13E-4|Clearance:0.11||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:GLC7(YER133W)|FD-Score:4.3|P-value:8.63E-6|Clearance:0.33||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:LSM2(YBL026W)|FD-Score:3.37|P-value:3.70E-4|Clearance:0.1||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:PCF11(YDR228C)|FD-Score:3.82|P-value:6.63E-5|Clearance:0.05||SGD DESC:mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping Gene:RFA1(YAR007C)|FD-Score:3.77|P-value:8.26E-5|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination Gene:RNA14(YMR061W)|FD-Score:3.27|P-value:5.30E-4|Clearance:0.13||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RRP15(YPR143W)|FD-Score:7.13|P-value:5.10E-13|Clearance:1.82||SGD DESC:Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs Gene:RRP45(YDR280W)|FD-Score:3.77|P-value:8.31E-5|Clearance:0.08||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:SPT6(YGR116W)|FD-Score:-3.23|P-value:6.12E-4|Clearance:0||SGD DESC:Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region Gene:TAF12(YDR145W)|FD-Score:4.75|P-value:1.03E-6|Clearance:0.45||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TOR2(YKL203C)|FD-Score:3.96|P-value:3.67E-5|Clearance:0.04||SGD DESC:PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication Gene:UGP1(YKL035W)|FD-Score:3.58|P-value:1.73E-4|Clearance:0.2||SGD DESC:UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication Gene:VRG4(YGL225W)|FD-Score:3.93|P-value:4.28E-5|Clearance:0.11||SGD DESC:Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi Gene:YNL114C(YNL114C_d)|FD-Score:-3.7|P-value:1.07E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Gene:YNL150W(YNL150W_d)|FD-Score:-3.25|P-value:5.85E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking Gene:YPR142C(YPR142C_d)|FD-Score:5.31|P-value:5.55E-8|Clearance:0.56||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 226033
Download HOP data (tab-delimited text)  (excel)
Gene:ALG6(YOR002W)|FD-Score:3.85|P-value:5.86E-5||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:ARG3(YJL088W)|FD-Score:4.19|P-value:1.38E-5||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ARO4(YBR249C)|FD-Score:4.5|P-value:3.46E-6||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan; relative distribution to the nucleus increases upon DNA replication stress Gene:ATF1(YOR377W)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Alcohol acetyltransferase; responsible for the major part of volatile acetate ester production during fermentation; main enzyme involved in terpenyl acetate synthesis; potential roles in lipid and sterol metabolism Gene:BIO4(YNR057C)|FD-Score:3.23|P-value:6.25E-4||SGD DESC:Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels Gene:BTS1(YPL069C)|FD-Score:5.85|P-value:2.44E-9||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:COG5(YNL051W)|FD-Score:-4.03|P-value:2.77E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CWH41(YGL027C)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress Gene:DAL7(YIR031C)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DLD3(YEL071W)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:D-lactate dehydrogenase, part of the retrograde regulon which consists of genes whose expression is stimulated by damage to mitochondria and reduced in cells grown with glutamate as the sole nitrogen source, located in the cytoplasm Gene:FUR4(YBR021W)|FD-Score:10.2|P-value:1.25E-24||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GCG1(YER163C_p)|FD-Score:4.35|P-value:6.70E-6||SGD DESC:Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle Gene:GDA1(YEL042W)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate Gene:GET4(YOR164C)|FD-Score:-3.23|P-value:6.17E-4||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Mdy2p; highly conserved across species and homologous to human gene C7orf20 Gene:GLN3(YER040W)|FD-Score:-3.66|P-value:1.25E-4||SGD DESC:Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source Gene:GSY1(YFR015C)|FD-Score:5.18|P-value:1.09E-7||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase; GSY1 has a paralog, GSY2, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:GTR1(YML121W)|FD-Score:3.94|P-value:4.06E-5||SGD DESC:Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB Gene:GTR2(YGR163W)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:IDP1(YDL066W)|FD-Score:3.87|P-value:5.53E-5||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:IML3(YBR107C)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Outer kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1 Gene:IPT1(YDR072C)|FD-Score:3.19|P-value:7.19E-4||SGD DESC:Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin Gene:IRS4(YKR019C)|FD-Score:-4.33|P-value:7.36E-6||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LDB19(YOR322C)|FD-Score:5.19|P-value:1.03E-7||SGD DESC:Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye Gene:LGE1(YPL055C)|FD-Score:6.11|P-value:4.88E-10||SGD DESC:Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division Gene:LTE1(YAL024C)|FD-Score:3.85|P-value:5.91E-5||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MEI4(YER044C-A)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores Gene:MRP8(YKL142W)|FD-Score:-3.28|P-value:5.22E-4||SGD DESC:Protein of unknown function; undergoes sumoylation; transcription induced under cell wall stress; protein levels are reduced under anaerobic conditions; protein abundance increases in response to DNA replication stress; originally thought to be a mitochondrial ribosomal protein based on sequence analysis Gene:MSN5(YDR335W)|FD-Score:-3.39|P-value:3.55E-4||SGD DESC:Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm Gene:NAB6(YML117W)|FD-Score:3.24|P-value:5.96E-4||SGD DESC:Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP Gene:NEW1(YPL226W)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:NTG1(YAL015C)|FD-Score:3.12|P-value:8.99E-4||SGD DESC:DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication Gene:OM14(YBR230C)|FD-Score:3.89|P-value:4.98E-5||SGD DESC:Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron Gene:ORM2(YLR350W)|FD-Score:-4|P-value:3.12E-5||SGD DESC:Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress Gene:PHO89(YBR296C)|FD-Score:-4.21|P-value:1.27E-5||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:PIN4(YBL051C)|FD-Score:-3.76|P-value:8.50E-5||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:RGL1(YPL066W)|FD-Score:3.87|P-value:5.41E-5||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RGP1(YDR137W)|FD-Score:-3.51|P-value:2.27E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RPL8A(YHL033C)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication Gene:RPS22B(YLR367W)|FD-Score:3.15|P-value:8.09E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication Gene:RRG9(YNL213C)|FD-Score:4.68|P-value:1.41E-6||SGD DESC:Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RSM22(YKL155C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase Gene:RTF1(YGL244W)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:SBE2(YDR351W)|FD-Score:4.58|P-value:2.37E-6||SGD DESC:Protein involved in the transport of cell wall components from the Golgi to the cell surface; required for bud growth Gene:SCW10(YMR305C)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on mutant phenotype and its regulation by Ste12p Gene:SEE1(YIL064W)|FD-Score:-3.82|P-value:6.75E-5||SGD DESC:Probable lysine methyltransferase involved in the dimethylation of eEF1A (Tef1p/Tef2p); sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport Gene:SGF29(YCL010C)|FD-Score:9.58|P-value:4.71E-22||SGD DESC:Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation Gene:SLO1(YER180C-A)|FD-Score:-3.39|P-value:3.52E-4||SGD DESC:Protein interacting with Arl3p, which is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO Gene:SNF6(YHL025W)|FD-Score:-3.13|P-value:8.89E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:SNZ2(YNL333W)|FD-Score:4.82|P-value:7.01E-7||SGD DESC:Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p Gene:SPF1(YEL031W)|FD-Score:4.89|P-value:5.13E-7||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SRC1(YML034W)|FD-Score:9.35|P-value:4.59E-21||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SSD1(YDR293C)|FD-Score:3.76|P-value:8.43E-5||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSN2(YDR443C)|FD-Score:-3.09|P-value:9.87E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:SWD3(YBR175W)|FD-Score:4.8|P-value:7.88E-7||SGD DESC:Essential subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5 Gene:TDH3(YGR192C)|FD-Score:-4.18|P-value:1.48E-5||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; TDH3 has a paralog, TDH2, that arose from the whole genome duplication Gene:TIR4(YOR009W)|FD-Score:4.05|P-value:2.59E-5||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth; transcription is also induced by cold shock Gene:TPK1(YJL164C)|FD-Score:-3.38|P-value:3.69E-4||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication Gene:TPO4(YOR273C)|FD-Score:3.22|P-value:6.44E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TSC3(YBR058C-A)|FD-Score:5.33|P-value:4.87E-8||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBP11(YKR098C)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin from ubiquitinated proteins Gene:VPS25(YJR102C)|FD-Score:-3.31|P-value:4.63E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS36(YLR417W)|FD-Score:-3.31|P-value:4.61E-4||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:YBL008W-A(YBL008W-A_p)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR239C(YDR239C)|FD-Score:5.82|P-value:2.91E-9||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YHL037C(YHL037C_d)|FD-Score:4.43|P-value:4.63E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR262W(YMR262W_p)|FD-Score:3.11|P-value:9.36E-4||SGD DESC:Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene Gene:YPR097W(YPR097W)|FD-Score:4.08|P-value:2.24E-5||SGD DESC:Protein that contains a Phox homology (PX) domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPR123C(YPR123C_d)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YSP1(YHR155W)|FD-Score:3.76|P-value:8.48E-5||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication Gene:ALG6(YOR002W)|FD-Score:3.85|P-value:5.86E-5||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:ARG3(YJL088W)|FD-Score:4.19|P-value:1.38E-5||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ARO4(YBR249C)|FD-Score:4.5|P-value:3.46E-6||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan; relative distribution to the nucleus increases upon DNA replication stress Gene:ATF1(YOR377W)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Alcohol acetyltransferase; responsible for the major part of volatile acetate ester production during fermentation; main enzyme involved in terpenyl acetate synthesis; potential roles in lipid and sterol metabolism Gene:BIO4(YNR057C)|FD-Score:3.23|P-value:6.25E-4||SGD DESC:Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels Gene:BTS1(YPL069C)|FD-Score:5.85|P-value:2.44E-9||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:COG5(YNL051W)|FD-Score:-4.03|P-value:2.77E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CWH41(YGL027C)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress Gene:DAL7(YIR031C)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DLD3(YEL071W)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:D-lactate dehydrogenase, part of the retrograde regulon which consists of genes whose expression is stimulated by damage to mitochondria and reduced in cells grown with glutamate as the sole nitrogen source, located in the cytoplasm Gene:FUR4(YBR021W)|FD-Score:10.2|P-value:1.25E-24||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GCG1(YER163C_p)|FD-Score:4.35|P-value:6.70E-6||SGD DESC:Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle Gene:GDA1(YEL042W)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate Gene:GET4(YOR164C)|FD-Score:-3.23|P-value:6.17E-4||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Mdy2p; highly conserved across species and homologous to human gene C7orf20 Gene:GLN3(YER040W)|FD-Score:-3.66|P-value:1.25E-4||SGD DESC:Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source Gene:GSY1(YFR015C)|FD-Score:5.18|P-value:1.09E-7||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase; GSY1 has a paralog, GSY2, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:GTR1(YML121W)|FD-Score:3.94|P-value:4.06E-5||SGD DESC:Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB Gene:GTR2(YGR163W)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:IDP1(YDL066W)|FD-Score:3.87|P-value:5.53E-5||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:IML3(YBR107C)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Outer kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1 Gene:IPT1(YDR072C)|FD-Score:3.19|P-value:7.19E-4||SGD DESC:Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin Gene:IRS4(YKR019C)|FD-Score:-4.33|P-value:7.36E-6||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LDB19(YOR322C)|FD-Score:5.19|P-value:1.03E-7||SGD DESC:Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye Gene:LGE1(YPL055C)|FD-Score:6.11|P-value:4.88E-10||SGD DESC:Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division Gene:LTE1(YAL024C)|FD-Score:3.85|P-value:5.91E-5||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MEI4(YER044C-A)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores Gene:MRP8(YKL142W)|FD-Score:-3.28|P-value:5.22E-4||SGD DESC:Protein of unknown function; undergoes sumoylation; transcription induced under cell wall stress; protein levels are reduced under anaerobic conditions; protein abundance increases in response to DNA replication stress; originally thought to be a mitochondrial ribosomal protein based on sequence analysis Gene:MSN5(YDR335W)|FD-Score:-3.39|P-value:3.55E-4||SGD DESC:Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm Gene:NAB6(YML117W)|FD-Score:3.24|P-value:5.96E-4||SGD DESC:Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP Gene:NEW1(YPL226W)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:NTG1(YAL015C)|FD-Score:3.12|P-value:8.99E-4||SGD DESC:DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication Gene:OM14(YBR230C)|FD-Score:3.89|P-value:4.98E-5||SGD DESC:Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron Gene:ORM2(YLR350W)|FD-Score:-4|P-value:3.12E-5||SGD DESC:Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress Gene:PHO89(YBR296C)|FD-Score:-4.21|P-value:1.27E-5||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:PIN4(YBL051C)|FD-Score:-3.76|P-value:8.50E-5||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:RGL1(YPL066W)|FD-Score:3.87|P-value:5.41E-5||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RGP1(YDR137W)|FD-Score:-3.51|P-value:2.27E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RPL8A(YHL033C)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication Gene:RPS22B(YLR367W)|FD-Score:3.15|P-value:8.09E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication Gene:RRG9(YNL213C)|FD-Score:4.68|P-value:1.41E-6||SGD DESC:Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RSM22(YKL155C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase Gene:RTF1(YGL244W)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:SBE2(YDR351W)|FD-Score:4.58|P-value:2.37E-6||SGD DESC:Protein involved in the transport of cell wall components from the Golgi to the cell surface; required for bud growth Gene:SCW10(YMR305C)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on mutant phenotype and its regulation by Ste12p Gene:SEE1(YIL064W)|FD-Score:-3.82|P-value:6.75E-5||SGD DESC:Probable lysine methyltransferase involved in the dimethylation of eEF1A (Tef1p/Tef2p); sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport Gene:SGF29(YCL010C)|FD-Score:9.58|P-value:4.71E-22||SGD DESC:Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation Gene:SLO1(YER180C-A)|FD-Score:-3.39|P-value:3.52E-4||SGD DESC:Protein interacting with Arl3p, which is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO Gene:SNF6(YHL025W)|FD-Score:-3.13|P-value:8.89E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:SNZ2(YNL333W)|FD-Score:4.82|P-value:7.01E-7||SGD DESC:Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p Gene:SPF1(YEL031W)|FD-Score:4.89|P-value:5.13E-7||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SRC1(YML034W)|FD-Score:9.35|P-value:4.59E-21||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SSD1(YDR293C)|FD-Score:3.76|P-value:8.43E-5||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSN2(YDR443C)|FD-Score:-3.09|P-value:9.87E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:SWD3(YBR175W)|FD-Score:4.8|P-value:7.88E-7||SGD DESC:Essential subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5 Gene:TDH3(YGR192C)|FD-Score:-4.18|P-value:1.48E-5||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; TDH3 has a paralog, TDH2, that arose from the whole genome duplication Gene:TIR4(YOR009W)|FD-Score:4.05|P-value:2.59E-5||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth; transcription is also induced by cold shock Gene:TPK1(YJL164C)|FD-Score:-3.38|P-value:3.69E-4||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication Gene:TPO4(YOR273C)|FD-Score:3.22|P-value:6.44E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TSC3(YBR058C-A)|FD-Score:5.33|P-value:4.87E-8||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBP11(YKR098C)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin from ubiquitinated proteins Gene:VPS25(YJR102C)|FD-Score:-3.31|P-value:4.63E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS36(YLR417W)|FD-Score:-3.31|P-value:4.61E-4||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:YBL008W-A(YBL008W-A_p)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR239C(YDR239C)|FD-Score:5.82|P-value:2.91E-9||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YHL037C(YHL037C_d)|FD-Score:4.43|P-value:4.63E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR262W(YMR262W_p)|FD-Score:3.11|P-value:9.36E-4||SGD DESC:Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene Gene:YPR097W(YPR097W)|FD-Score:4.08|P-value:2.24E-5||SGD DESC:Protein that contains a Phox homology (PX) domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPR123C(YPR123C_d)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YSP1(YHR155W)|FD-Score:3.76|P-value:8.48E-5||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YPR143W7.135.10E-131.82RRP15Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs
YAL038W5.315.52E-80.00CDC19Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication
YPR142C_d5.315.55E-80.56YPR142C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing
YDR145W4.751.03E-60.45TAF12Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A
YER133W4.308.63E-60.33GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YKL203C3.963.67E-50.04TOR2PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
YGL225W3.934.28E-50.11VRG4Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi
YDR228C3.826.63E-50.05PCF11mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping
YAR007C3.778.26E-50.00RFA1Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination
YDR280W3.778.31E-50.08RRP45Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
YHR166C3.691.13E-40.11CDC23Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YKL035W3.581.73E-40.20UGP1UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication
YBL026W3.373.70E-40.10LSM2Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YMR061W3.275.30E-40.13RNA14Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
YJL081C3.148.32E-40.07ARP4Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR021W10.201.25E-24FUR4Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation
YCL010C9.584.71E-22SGF29Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation
YML034W9.354.59E-21SRC1Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance
YPL055C6.114.88E-10LGE1Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division
YPL069C5.852.44E-9BTS1Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
YDR239C5.822.91E-9YDR239CProtein of unknown function that may interact with ribosomes, based on co-purification experiments
YBR058C-A5.334.87E-8TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YOR322C5.191.03E-7LDB19Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye
YFR015C5.181.09E-7GSY1Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase; GSY1 has a paralog, GSY2, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YEL031W4.895.13E-7SPF1P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1
YNL333W4.827.01E-7SNZ2Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p
YBR175W4.807.88E-7SWD3Essential subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5
YNL213C4.681.41E-6RRG9Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YDR351W4.582.37E-6SBE2Protein involved in the transport of cell wall components from the Golgi to the cell surface; required for bud growth
YBR249C4.503.46E-6ARO43-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan; relative distribution to the nucleus increases upon DNA replication stress

GO enrichment analysis for SGTC_305
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2002.90E-54SGTC_23519026858 72.3 μMChembridge (Fragment library)14093000.103448RNA processing & uracil transport
0.1782.39E-43SGTC_1471k673-0312 37.0 μMChemDiv (Drug-like library)240998840.169231RNA processing & uracil transport
0.1562.00E-33SGTC_9851493-0307 8.7 μMChemDiv (Drug-like library)32782740.0677966TSC3-RPN4
0.1533.41E-32SGTC_20825219693 150.0 μMChembridge (Fragment library)28346490.140351RNA processing & uracil transport
0.1511.24E-31SGTC_397arbidol 50.0 μMMiscellaneous1314100.0987654cell wall
0.1483.96E-30SGTC_23939031399 185.9 μMChembridge (Fragment library)15266230.0666667RNA processing & uracil transport
0.1461.24E-29SGTC_1817st052116 64.4 μMTimTec (Natural product derivative library)43613980.0909091RNA processing & uracil transport
0.1381.82E-26SGTC_9231900-2434 73.3 μMChemDiv (Drug-like library)59105700.0833333RNA processing & uracil transport
0.1369.45E-26SGTC_9841492-0255 2.9 μMChemDiv (Drug-like library)15851980.118644SWF1 & branched chain AA biosynthesis
0.1331.49E-24SGTC_12021988-0068 29.6 μMChemDiv (Drug-like library)28504800.123288RNA processing & uracil transport
0.1315.38E-24SGTC_6390833-0042 114.0 μMChemDiv (Drug-like library)59789110.0967742
0.1315.95E-24SGTC_8093998-0021 90.0 μMChemDiv (Drug-like library)7154850.0923077RNA processing & uracil transport
0.1301.02E-23SGTC_1167k781-0610 148.0 μMChemDiv (Drug-like library)45251690.145161RNA processing & uracil transport
0.1301.07E-23SGTC_1814st052110 31.5 μMTimTec (Natural product derivative library)6741630.0615385RNA processing & uracil transport
0.1301.07E-23SGTC_398bronopol 33.3 μMMiscellaneous24500.113636RNA processing & uracil transport

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1696st02559134.79 μM0.4117652787147TimTec (Natural product derivative library)285.276440.49337SWF1 & branched chain AA biosynthesis
SGTC_1764st04450293.5 μM0.3902448407TimTec (Natural product derivative library)214.223.27413
SGTC_230213-001140 μM0.333333627608ChemDiv (Drug-like library)304.215123.06317copper-dependent oxidative stress
SGTC_2843900089445.46 μM0.3333332242070Chembridge (Drug-like library)289.2617833.10924
SGTC_5983937-023620.2 μM0.3207551379313ChemDiv (Drug-like library)354.80864.33515ERG2
SGTC_8470342-02745.54 μM0.31818218097ChemDiv (Drug-like library)218.165641.28204
SGTC_340469-068381.75 μM0.3076923690923ChemDiv (Drug-like library)337.307942.59217SWF1 & branched chain AA biosynthesis
SGTC_13121218-2183165 μM0.2931036744073ChemDiv (Drug-like library)299.281420.96225
SGTC_15141189-1591158 μM0.283019767698ChemDiv (Drug-like library)320.363543.6715
SGTC_2674-Nitroquinoline-1-oxide (4-NQO)44.4 nM0.2826095955Miscellaneous190.155541.06603DNA damage response