9094636

[4-[(2-chlorophenyl)methyl]piperazin-1-yl]-(5-methylthiophen-3-yl)methanone

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_3055
Screen concentration 49.5 μM
Source Chembridge (Drug-like library)
PubChem CID 25236176
SMILES CC1=CC(=CS1)C(=O)N2CCN(CC2)CC3=CC=CC=C3Cl
Standardized SMILES Cc1cc(cs1)C(=O)N2CCN(Cc3ccccc3Cl)CC2
Molecular weight 334.8636
ALogP 3.26
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 15.85
% growth inhibition (Hom. pool) 5.7


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 25236176
Download HIP data (tab-delimited text)  (excel)
Gene:CCT4(YDL143W)|FD-Score:3.25|P-value:5.72E-4|Clearance:0.09||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CMD1(YBR109C)|FD-Score:4.14|P-value:1.70E-5|Clearance:0.09||SGD DESC:Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin Gene:GAB1(YLR459W)|FD-Score:4.32|P-value:7.77E-6|Clearance:0.18||SGD DESC:GPI transamidase subunit, involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or of the lipid portion of GPI Gene:GPN2(YOR262W)|FD-Score:3.3|P-value:4.79E-4|Clearance:0.05||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion Gene:HCA4(YJL033W)|FD-Score:-3.2|P-value:6.86E-4|Clearance:0||SGD DESC:DEAD box RNA helicase; high-copy number suppression of a U14 snoRNA processing mutant suggests an involvement in 18S rRNA synthesis Gene:HYP2(YEL034W)|FD-Score:3.96|P-value:3.75E-5|Clearance:0.27||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:IMP3(YHR148W)|FD-Score:3.16|P-value:7.83E-4|Clearance:0.1||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA Gene:NOP14(YDL148C)|FD-Score:-5.31|P-value:5.35E-8|Clearance:0||SGD DESC:Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:ORC2(YBR060C)|FD-Score:-3.79|P-value:7.48E-5|Clearance:0||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p Gene:ORC4(YPR162C)|FD-Score:4.05|P-value:2.53E-5|Clearance:0.01||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:PRP43(YGL120C)|FD-Score:4|P-value:3.14E-5|Clearance:0.04||SGD DESC:RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome Gene:PWP2(YCR057C)|FD-Score:-3.51|P-value:2.25E-4|Clearance:0||SGD DESC:Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis Gene:RPL10(YLR075W)|FD-Score:3.69|P-value:1.13E-4|Clearance:0.22||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPL15A(YLR029C)|FD-Score:-4.29|P-value:8.92E-6|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPL32(YBL092W)|FD-Score:4.05|P-value:2.61E-5|Clearance:0.04||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPN11(YFR004W)|FD-Score:3.17|P-value:7.71E-4|Clearance:0||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:RSC6(YCR052W)|FD-Score:3.37|P-value:3.78E-4|Clearance:0.07||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:UTP6(YDR449C)|FD-Score:5.2|P-value:9.82E-8|Clearance:0.26||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YDL163W(YDL163W_d)|FD-Score:-3.89|P-value:5.01E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase Gene:YGL069C(YGL069C_d)|FD-Score:4.95|P-value:3.76E-7|Clearance:0.63||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance Gene:YJL086C(YJL086C_d)|FD-Score:9.36|P-value:3.83E-21|Clearance:4.16||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YKL083W(YKL083W_d)|FD-Score:-4.86|P-value:5.98E-7|Clearance:0||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene RRP14 Gene:YLL037W(YLL037W_d)|FD-Score:3.47|P-value:2.59E-4|Clearance:0.1||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential PRP19 gene encoding an RNA splicing factor Gene:CCT4(YDL143W)|FD-Score:3.25|P-value:5.72E-4|Clearance:0.09||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CMD1(YBR109C)|FD-Score:4.14|P-value:1.70E-5|Clearance:0.09||SGD DESC:Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin Gene:GAB1(YLR459W)|FD-Score:4.32|P-value:7.77E-6|Clearance:0.18||SGD DESC:GPI transamidase subunit, involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or of the lipid portion of GPI Gene:GPN2(YOR262W)|FD-Score:3.3|P-value:4.79E-4|Clearance:0.05||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion Gene:HCA4(YJL033W)|FD-Score:-3.2|P-value:6.86E-4|Clearance:0||SGD DESC:DEAD box RNA helicase; high-copy number suppression of a U14 snoRNA processing mutant suggests an involvement in 18S rRNA synthesis Gene:HYP2(YEL034W)|FD-Score:3.96|P-value:3.75E-5|Clearance:0.27||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:IMP3(YHR148W)|FD-Score:3.16|P-value:7.83E-4|Clearance:0.1||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA Gene:NOP14(YDL148C)|FD-Score:-5.31|P-value:5.35E-8|Clearance:0||SGD DESC:Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:ORC2(YBR060C)|FD-Score:-3.79|P-value:7.48E-5|Clearance:0||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p Gene:ORC4(YPR162C)|FD-Score:4.05|P-value:2.53E-5|Clearance:0.01||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:PRP43(YGL120C)|FD-Score:4|P-value:3.14E-5|Clearance:0.04||SGD DESC:RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome Gene:PWP2(YCR057C)|FD-Score:-3.51|P-value:2.25E-4|Clearance:0||SGD DESC:Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis Gene:RPL10(YLR075W)|FD-Score:3.69|P-value:1.13E-4|Clearance:0.22||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPL15A(YLR029C)|FD-Score:-4.29|P-value:8.92E-6|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPL32(YBL092W)|FD-Score:4.05|P-value:2.61E-5|Clearance:0.04||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPN11(YFR004W)|FD-Score:3.17|P-value:7.71E-4|Clearance:0||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:RSC6(YCR052W)|FD-Score:3.37|P-value:3.78E-4|Clearance:0.07||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:UTP6(YDR449C)|FD-Score:5.2|P-value:9.82E-8|Clearance:0.26||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YDL163W(YDL163W_d)|FD-Score:-3.89|P-value:5.01E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase Gene:YGL069C(YGL069C_d)|FD-Score:4.95|P-value:3.76E-7|Clearance:0.63||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance Gene:YJL086C(YJL086C_d)|FD-Score:9.36|P-value:3.83E-21|Clearance:4.16||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YKL083W(YKL083W_d)|FD-Score:-4.86|P-value:5.98E-7|Clearance:0||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene RRP14 Gene:YLL037W(YLL037W_d)|FD-Score:3.47|P-value:2.59E-4|Clearance:0.1||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential PRP19 gene encoding an RNA splicing factor

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 25236176
Download HOP data (tab-delimited text)  (excel)
Gene:ABP140(YOR239W)|FD-Score:3.22|P-value:6.44E-4||SGD DESC:AdoMet-dependent tRNA methyltransferase and actin binding protein; C-terminal domain is responsible for 3-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA-Thr and tRNA-Ser and contains an S-adenosylmethionine (AdoMet) binding motif; N-terminal actin binding sequence interacts with actin filaments and localizes to actin patches and cables; N- and C-terminal domains are encoded in separate ORFs that are translated into one protein via a +1 frameshift Gene:AKR1(YDR264C)|FD-Score:3.1|P-value:9.83E-4||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:ARC1(YGL105W)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress Gene:ATG12(YBR217W)|FD-Score:-3.14|P-value:8.42E-4||SGD DESC:Conserved ubiquitin-like modifier involved in autophagy and the Cvt pathway; conjugated to Atg5p to form a complex involved in Atg8p lipidation; Atg12p-Atg5p also forms a complex with Atg16p that is required for autophagosome formation Gene:BEM1(YBR200W)|FD-Score:3.17|P-value:7.58E-4||SGD DESC:Protein containing SH3-domains, involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p Gene:BIO3(YNR058W)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:7,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:CSH1(YBR161W)|FD-Score:3.42|P-value:3.19E-4||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication Gene:CTR1(YPR124W)|FD-Score:3.89|P-value:5.00E-5||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:ERG4(YGL012W)|FD-Score:4.17|P-value:1.49E-5||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:GAS3(YMR215W)|FD-Score:-3.12|P-value:9.14E-4||SGD DESC:Low abundance, possibly inactive member of the GAS family of GPI-containing proteins; putative 1,3-beta-glucanosyltransferase with similarity to other GAS family members; localizes to the cell wall; mRNA induced during sporulation Gene:GSF2(YML048W)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression Gene:GUP1(YGL084C)|FD-Score:4.18|P-value:1.43E-5||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HFA1(YMR207C)|FD-Score:-3.56|P-value:1.82E-4||SGD DESC:Mitochondrial acetyl-coenzyme A carboxylase; catalyzes the production of malonyl-CoA in mitochondrial fatty acid biosynthesis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:HHO1(YPL127C)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Histone H1, linker histone with roles in meiosis and sporulation; decreasing levels early in sporulation may promote meiosis, and increasing levels during sporulation facilitate compaction of spore chromatin; binds to promoters and within genes in mature spores; may be recruited by Ume6p to promoter regions, contributing to transcriptional repression outside of meiosis; suppresses DNA repair involving homologous recombination Gene:HPF1(YOL155C)|FD-Score:3.85|P-value:5.84E-5||SGD DESC:Haze-protective mannoprotein that reduces the particle size of aggregated proteins in white wines Gene:IML2(YJL082W)|FD-Score:-3.27|P-value:5.38E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; IML2 has a paralog, YKR018C, that arose from the whole genome duplication Gene:LEE1(YPL054W)|FD-Score:-3.19|P-value:7.11E-4||SGD DESC:Zinc-finger protein of unknown function Gene:MCH4(YOL119C)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MDM20(YOL076W)|FD-Score:-4.46|P-value:4.05E-6||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MSO1(YNR049C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Probable component of the secretory vesicle docking complex; acts at a late step in secretion; shows genetic and physical interactions with Sec1p; required for prospore membrane formation during sporulation; relocalizes from bud neck to nucleus upon DNA replication stress Gene:MTC2(YKL098W)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Protein of unknown function; mtc2 is synthetically sick with cdc13-1 Gene:MUD1(YBR119W)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:U1 snRNP A protein, homolog of human U1-A; involved in nuclear mRNA splicing Gene:PAT1(YCR077C)|FD-Score:3.25|P-value:5.87E-4||SGD DESC:Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress Gene:PET100(YDR079W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Chaperone that specifically facilitates the assembly of cytochrome c oxidase, integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme Gene:PFK2(YMR205C)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:RAD34(YDR314C)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Protein involved in nucleotide excision repair (NER); homologous to RAD4 Gene:ROM1(YGR070W)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP Gene:RPL37B(YDR500C)|FD-Score:-3.61|P-value:1.56E-4||SGD DESC:Ribosomal 60S subunit protein L37B; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication Gene:RPS22B(YLR367W)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication Gene:RPS4B(YHR203C)|FD-Score:-3.38|P-value:3.60E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication Gene:RRG9(YNL213C)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RTC3(YHR087W)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress Gene:RTS1(YOR014W)|FD-Score:3.91|P-value:4.64E-5||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:RXT2(YBR095C)|FD-Score:-3.34|P-value:4.16E-4||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SCT1(YBL011W)|FD-Score:-3.13|P-value:8.72E-4||SGD DESC:Glycerol 3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase of the glycerolipid biosynthesis pathway, prefers 16-carbon fatty acids, similar to Gpt2p, gene is constitutively transcribed Gene:SDS3(YIL084C)|FD-Score:4.81|P-value:7.54E-7||SGD DESC:Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes Gene:SEG2(YKL105C_p)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:Eisosome component and paralog of Seg1p; likely plays only minor role in eisosome assembly; shown to interact with Seg1p; similar to A. gossypii SEG gene which is important for stabilizing eisosomes Gene:SLT2(YHR030C)|FD-Score:-5.28|P-value:6.44E-8||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SPT21(YMR179W)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SPT4(YGR063C)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SRB8(YCR081W)|FD-Score:-3.98|P-value:3.39E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:TCB2(YNL087W)|FD-Score:-3.3|P-value:4.83E-4||SGD DESC:ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains 3 calcium and lipid binding domains; mRNA is targeted to the bud Gene:TDA5(YLR426W_p)|FD-Score:4.16|P-value:1.57E-5||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TLG2(YOL018C)|FD-Score:5.22|P-value:8.96E-8||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TRE1(YPL176C)|FD-Score:3.15|P-value:8.09E-4||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:TRS85(YDR108W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:WTM2(YOR229W)|FD-Score:3.78|P-value:7.81E-5||SGD DESC:Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; involved in response to replication stress; contains WD repeats Gene:YAK1(YJL141C)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:Serine-threonine protein kinase; component of a glucose-sensing system that inhibits growth in response to glucose availability; upon nutrient deprivation Yak1p phosphorylates Pop2p to regulate mRNA deadenylation, the co-repressor Crf1p to inhibit transcription of ribosomal genes, and the stress-responsive transcription factors Hsf1p and Msn2p; nuclear localization negatively regulated by the Ras/PKA signaling pathway in the presence of glucose Gene:YAL056C-A(YAL056C-A_d)|FD-Score:3.15|P-value:8.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER130C(YER130C_p)|FD-Score:-3.18|P-value:7.33E-4||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YER181C(YER181C_d)|FD-Score:8.68|P-value:1.98E-18||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative data; extensively overlaps a Ty1 LTR; protein product is detected in highly purified mitochondria in high-throughput studies Gene:YKR041W(YKR041W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Protein of unknown function; localizes to the mitotic spindle; overexpression of YKR041W affects endocytic protein trafficking Gene:YMR196W(YMR196W_p)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YOR139C(YOR139C_d)|FD-Score:-3.43|P-value:2.97E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W Gene:YPL080C(YPL080C_d)|FD-Score:6.48|P-value:4.45E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL247C(YPL247C_p)|FD-Score:-3.2|P-value:6.81E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest Gene:ABP140(YOR239W)|FD-Score:3.22|P-value:6.44E-4||SGD DESC:AdoMet-dependent tRNA methyltransferase and actin binding protein; C-terminal domain is responsible for 3-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA-Thr and tRNA-Ser and contains an S-adenosylmethionine (AdoMet) binding motif; N-terminal actin binding sequence interacts with actin filaments and localizes to actin patches and cables; N- and C-terminal domains are encoded in separate ORFs that are translated into one protein via a +1 frameshift Gene:AKR1(YDR264C)|FD-Score:3.1|P-value:9.83E-4||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:ARC1(YGL105W)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress Gene:ATG12(YBR217W)|FD-Score:-3.14|P-value:8.42E-4||SGD DESC:Conserved ubiquitin-like modifier involved in autophagy and the Cvt pathway; conjugated to Atg5p to form a complex involved in Atg8p lipidation; Atg12p-Atg5p also forms a complex with Atg16p that is required for autophagosome formation Gene:BEM1(YBR200W)|FD-Score:3.17|P-value:7.58E-4||SGD DESC:Protein containing SH3-domains, involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p Gene:BIO3(YNR058W)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:7,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:CSH1(YBR161W)|FD-Score:3.42|P-value:3.19E-4||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication Gene:CTR1(YPR124W)|FD-Score:3.89|P-value:5.00E-5||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:ERG4(YGL012W)|FD-Score:4.17|P-value:1.49E-5||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:GAS3(YMR215W)|FD-Score:-3.12|P-value:9.14E-4||SGD DESC:Low abundance, possibly inactive member of the GAS family of GPI-containing proteins; putative 1,3-beta-glucanosyltransferase with similarity to other GAS family members; localizes to the cell wall; mRNA induced during sporulation Gene:GSF2(YML048W)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression Gene:GUP1(YGL084C)|FD-Score:4.18|P-value:1.43E-5||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HFA1(YMR207C)|FD-Score:-3.56|P-value:1.82E-4||SGD DESC:Mitochondrial acetyl-coenzyme A carboxylase; catalyzes the production of malonyl-CoA in mitochondrial fatty acid biosynthesis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:HHO1(YPL127C)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Histone H1, linker histone with roles in meiosis and sporulation; decreasing levels early in sporulation may promote meiosis, and increasing levels during sporulation facilitate compaction of spore chromatin; binds to promoters and within genes in mature spores; may be recruited by Ume6p to promoter regions, contributing to transcriptional repression outside of meiosis; suppresses DNA repair involving homologous recombination Gene:HPF1(YOL155C)|FD-Score:3.85|P-value:5.84E-5||SGD DESC:Haze-protective mannoprotein that reduces the particle size of aggregated proteins in white wines Gene:IML2(YJL082W)|FD-Score:-3.27|P-value:5.38E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; IML2 has a paralog, YKR018C, that arose from the whole genome duplication Gene:LEE1(YPL054W)|FD-Score:-3.19|P-value:7.11E-4||SGD DESC:Zinc-finger protein of unknown function Gene:MCH4(YOL119C)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MDM20(YOL076W)|FD-Score:-4.46|P-value:4.05E-6||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MSO1(YNR049C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Probable component of the secretory vesicle docking complex; acts at a late step in secretion; shows genetic and physical interactions with Sec1p; required for prospore membrane formation during sporulation; relocalizes from bud neck to nucleus upon DNA replication stress Gene:MTC2(YKL098W)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Protein of unknown function; mtc2 is synthetically sick with cdc13-1 Gene:MUD1(YBR119W)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:U1 snRNP A protein, homolog of human U1-A; involved in nuclear mRNA splicing Gene:PAT1(YCR077C)|FD-Score:3.25|P-value:5.87E-4||SGD DESC:Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress Gene:PET100(YDR079W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Chaperone that specifically facilitates the assembly of cytochrome c oxidase, integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme Gene:PFK2(YMR205C)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:RAD34(YDR314C)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Protein involved in nucleotide excision repair (NER); homologous to RAD4 Gene:ROM1(YGR070W)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP Gene:RPL37B(YDR500C)|FD-Score:-3.61|P-value:1.56E-4||SGD DESC:Ribosomal 60S subunit protein L37B; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication Gene:RPS22B(YLR367W)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication Gene:RPS4B(YHR203C)|FD-Score:-3.38|P-value:3.60E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication Gene:RRG9(YNL213C)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RTC3(YHR087W)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress Gene:RTS1(YOR014W)|FD-Score:3.91|P-value:4.64E-5||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:RXT2(YBR095C)|FD-Score:-3.34|P-value:4.16E-4||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SCT1(YBL011W)|FD-Score:-3.13|P-value:8.72E-4||SGD DESC:Glycerol 3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase of the glycerolipid biosynthesis pathway, prefers 16-carbon fatty acids, similar to Gpt2p, gene is constitutively transcribed Gene:SDS3(YIL084C)|FD-Score:4.81|P-value:7.54E-7||SGD DESC:Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes Gene:SEG2(YKL105C_p)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:Eisosome component and paralog of Seg1p; likely plays only minor role in eisosome assembly; shown to interact with Seg1p; similar to A. gossypii SEG gene which is important for stabilizing eisosomes Gene:SLT2(YHR030C)|FD-Score:-5.28|P-value:6.44E-8||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SPT21(YMR179W)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SPT4(YGR063C)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SRB8(YCR081W)|FD-Score:-3.98|P-value:3.39E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:TCB2(YNL087W)|FD-Score:-3.3|P-value:4.83E-4||SGD DESC:ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains 3 calcium and lipid binding domains; mRNA is targeted to the bud Gene:TDA5(YLR426W_p)|FD-Score:4.16|P-value:1.57E-5||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TLG2(YOL018C)|FD-Score:5.22|P-value:8.96E-8||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TRE1(YPL176C)|FD-Score:3.15|P-value:8.09E-4||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:TRS85(YDR108W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:WTM2(YOR229W)|FD-Score:3.78|P-value:7.81E-5||SGD DESC:Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; involved in response to replication stress; contains WD repeats Gene:YAK1(YJL141C)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:Serine-threonine protein kinase; component of a glucose-sensing system that inhibits growth in response to glucose availability; upon nutrient deprivation Yak1p phosphorylates Pop2p to regulate mRNA deadenylation, the co-repressor Crf1p to inhibit transcription of ribosomal genes, and the stress-responsive transcription factors Hsf1p and Msn2p; nuclear localization negatively regulated by the Ras/PKA signaling pathway in the presence of glucose Gene:YAL056C-A(YAL056C-A_d)|FD-Score:3.15|P-value:8.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER130C(YER130C_p)|FD-Score:-3.18|P-value:7.33E-4||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YER181C(YER181C_d)|FD-Score:8.68|P-value:1.98E-18||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative data; extensively overlaps a Ty1 LTR; protein product is detected in highly purified mitochondria in high-throughput studies Gene:YKR041W(YKR041W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Protein of unknown function; localizes to the mitotic spindle; overexpression of YKR041W affects endocytic protein trafficking Gene:YMR196W(YMR196W_p)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YOR139C(YOR139C_d)|FD-Score:-3.43|P-value:2.97E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W Gene:YPL080C(YPL080C_d)|FD-Score:6.48|P-value:4.45E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL247C(YPL247C_p)|FD-Score:-3.2|P-value:6.81E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJL086C_d9.363.83E-214.16YJL086C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1
YDR449C5.209.82E-80.26UTP6Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YGL069C_d4.953.76E-70.63YGL069C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance
YLR459W4.327.77E-60.18GAB1GPI transamidase subunit, involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or of the lipid portion of GPI
YBR109C4.141.70E-50.09CMD1Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin
YPR162C4.052.53E-50.01ORC4Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing
YBL092W4.052.61E-50.04RPL32Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog
YGL120C4.003.14E-50.04PRP43RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome
YEL034W3.963.75E-50.27HYP2Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication
YLR075W3.691.13E-40.22RPL10Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects
YLL037W_d3.472.59E-40.10YLL037W_dDubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential PRP19 gene encoding an RNA splicing factor
YCR052W3.373.78E-40.07RSC6Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p
YOR262W3.304.79E-40.05GPN2Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion
YDL143W3.255.72E-40.09CCT4Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YFR004W3.177.71E-40.00RPN11Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YER181C_d8.681.98E-18YER181C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative data; extensively overlaps a Ty1 LTR; protein product is detected in highly purified mitochondria in high-throughput studies
YPL080C_d6.484.45E-11YPL080C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YOL018C5.228.96E-8TLG2Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16)
YIL084C4.817.54E-7SDS3Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes
YDR314C4.261.03E-5RAD34Protein involved in nucleotide excision repair (NER); homologous to RAD4
YGL084C4.181.43E-5GUP1Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport
YGL012W4.171.49E-5ERG4C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol
YLR426W_p4.161.57E-5TDA5_pPutative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele
YOR014W3.914.64E-5RTS1B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A
YPR124W3.895.00E-5CTR1High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress
YOL155C3.855.84E-5HPF1Haze-protective mannoprotein that reduces the particle size of aggregated proteins in white wines
YOR229W3.787.81E-5WTM2Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; involved in response to replication stress; contains WD repeats
YDR079W3.701.07E-4PET100Chaperone that specifically facilitates the assembly of cytochrome c oxidase, integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme
YBR119W3.681.15E-4MUD1U1 snRNP A protein, homolog of human U1-A; involved in nuclear mRNA splicing
YDR108W3.671.22E-4TRS85Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role

GO enrichment analysis for SGTC_3055
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1255.99E-22SGTC_8510394-0702 453.0 μMChemDiv (Drug-like library)7626460.0857143mitochondrial processes
0.1048.06E-16SGTC_14813346-2004 36.0 μMChemDiv (Drug-like library)27705220.142857
0.0872.14E-11SGTC_1098clofazimine 15.1 μMNIH Clinical Collection27940.107143heme biosynthesis & mitochondrial translocase
0.0831.52E-10SGTC_120rhodamine 6G 52.3 μMChemDiv (Drug-like library)652110.130435mitochondrial processes
0.0822.35E-10SGTC_14780453-0656 35.7 μMChemDiv (Drug-like library)52584960.15942
0.0781.64E-9SGTC_21836420625 130.5 μMChembridge (Fragment library)28976190.0933333mitochondrial processes
0.0758.86E-9SGTC_22127123476 200.0 μMChembridge (Fragment library)10857150.0923077
0.0723.19E-8SGTC_29349036370 36.9 μMChembridge (Drug-like library)64628000.213333
0.0714.83E-8SGTC_2129girgensonine 121.1 μMChembridge (Fragment library)4426380.0923077heme biosynthesis & mitochondrial translocase
0.0715.59E-8SGTC_8080711-0032 135.0 μMChemDiv (Drug-like library)42525550.140845
0.0705.94E-8SGTC_1843st055809 45.5 μMTimTec (Natural product derivative library)164138350.115385
0.0699.72E-8SGTC_24405628896 157.8 μMMiscellaneous55220480.0821918
0.0691.26E-7SGTC_30039075612 71.4 μMChembridge (Drug-like library)65019930.0864198
0.0663.66E-7SGTC_7450469-0705 296.0 μMChemDiv (Drug-like library)34867360.125mitochondrial processes
0.0663.84E-7SGTC_2575plumbagin 510.0 nMTimTec (Pure natural product library)102050.111111superoxide

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3044909319349.47 μM0.64705925236277Chembridge (Drug-like library)334.863563.54803
SGTC_3031908876249.47 μM0.604167205253Chembridge (Drug-like library)349.254324.11302
SGTC_3139909502049.47 μM0.58490625236292Chembridge (Drug-like library)318.797962.98903
SGTC_22867945697125 μM0.5272731245733Chembridge (Fragment library)287.379982.06904
SGTC_3040909189449.47 μM0.50877225236966Chembridge (Drug-like library)328.471663.71503DNA intercalators
SGTC_3049909222349.47 μM0.4482768623917Chembridge (Drug-like library)328.835843.93502
SGTC_3137909735249.47 μM0.43333344120435Chembridge (Drug-like library)371.32453.9313
SGTC_3045909295049.47 μM0.42372944120432Chembridge (Drug-like library)365.296784.31712
SGTC_14354092-078956.7 μM0.4107142248285ChemDiv (Drug-like library)344.87834.44103plasma membrane duress
SGTC_3054909439749.47 μM0.4107142598354Chembridge (Drug-like library)330.422883.69302fatty acid desaturase (OLE1)